CN104642298A - Miniature loading system for cryopreservation of biological materials - Google Patents
Miniature loading system for cryopreservation of biological materials Download PDFInfo
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- CN104642298A CN104642298A CN201310594977.6A CN201310594977A CN104642298A CN 104642298 A CN104642298 A CN 104642298A CN 201310594977 A CN201310594977 A CN 201310594977A CN 104642298 A CN104642298 A CN 104642298A
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- cryovial
- extension rod
- loading system
- frozen
- miniature
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Abstract
The invention provides a miniature loading system for cryopreservation of biological materials. The miniature loading system comprises a refrigeration unit which contains a refrigeration pipe and a socketed extension rod. The refrigeration pipe is hollow and the bottom of the refrigeration unit is provided with an elastic sealing member which makes the bottom of the refrigeration pipe to be sealed. The top of the refrigeration pipe is opened. The bottom of the socketed extension rod is provided with a bottom pin which is used for sealing by being inserted into the refrigeration pipe from the opening at the top of the refrigeration pipe. By the adoption of the miniature loading system for cryopreservation of biological materials, it is guaranteed that operations such as refrigeration load, labeling, storage, distinguishing, fetching, unfreezing and the like, are simpler and more convenient, and occupied space of a carrier device can be minimized more remarkably. In addition, cryopreservation is safer.
Description
Technical field
The present invention relates to a kind of miniature loading system frozen for biomaterial, particularly relate to the miniature loading system of a kind of liquid sample such as biological cell or human ancillary reproductive seminal fluid.
Background technology
At present, known biomaterial freezing carrier system has many kinds, and wherein for the sample having liquid more than 1ml, the short cylindrical type cryopreservation tube usually adopted, its one end is cecum, and the other end is closed with screw thread or click on fashion by block.This container is usually comparatively large, and volume generally more than 1 milliliter, and is stored on the layer-stepping carrier of liquid nitrogen container for box-packed year by grid mostly, also has the form of putting 3-4 pipe by the every bar cramp of aluminum clamping bar to deposit.The sample that the frozen and seminal fluid of current most cells is frozen etc. is mixed in liquid nutrient medium adopts this form.
But the frozen device storage area of current above-mentioned various sample takies larger.Freezing for semen sample in Issues of Human Assisted Reproductive Technologies, no matter it is grid box-like formula or aluminum clamping bar form, and the largest tube number that 50 liters of liquid nitrogen container can store is about 800 pipes.Simultaneously, no matter be grid box-like formula or aluminum clamping bar form, when thawing at every turn, well-bucket or aluminum clamping bar all to be proposed, this make to be in well-bucket upper strata but the sample do not thawed all will to stand liquid nitrogen outer of short duration but still have the nearly thawed state of certain influence, affect frozen effect.
Summary of the invention
In order to overcome the deficiency of existing biomaterial freezing carrier system in storage area utilization ratio and in frozen effect, the invention provides a kind of biomaterial freezing carrier system, with guarantee freezing loading, mark, storage, resolution and taking-up and each operation such as to thaw more simple and convenient while, the space shared by carrier arrangement can also be reduced more significantly, and frozen safer.
First aspect of the present invention is to provide a kind of miniature loading system frozen for biomaterial, comprises freezing unit, and described freezing unit comprises cryovial and socket extension rod;
Described cryovial is that the bottom of hollow is provided with elastic sealing element, makes the bottom end seal of cryovial; The top end opening of described cryovial;
The bottom of described socket extension rod is provided with bottom plug, and described bottom plug is used for inserting in cryovial from the top end opening of cryovial forming sealing.
Described cryovial is slim-lined construction, and its external diameter is preferably≤and 8.5mm is more preferably 4-8.5mm, is more preferably 5-7mm.
Preferably, described socket extension rod is the tubular structure of all or part of hollow, closed at both ends.
Further preferably, the external diameter of described socket extension rod is identical with the external diameter of cryovial.
Further preferably, on the top of described socket extension rod and/or top sidewall and/or bottom sidewall be provided with liquid-through hole, described liquid-through hole is communicated with hollow structure and the external environment condition of socket extension rod.
Preferably, described elastic sealing element is made up of rubber.
Preferably, described miniature loading system also comprises liquid sucking device, described liquid sucking device comprises takes out aerating device for what be tightly connected with the top end opening of cryovial, and for running through elastic sealing element thus the aspirating needle be communicated with external environment condition by the hollow structure of cryovial.
Preferably, institute's liquid sucking device is syringe, and wherein, injector cartridge is formed takes out aerating device, and the syringe needle of syringe forms aspirating needle.
Further preferably, the syringe needle of described syringe is 2.
Preferably, the aerating device of taking out of described syringe is injector cartridge, directly forms socket extension rod again simultaneously.
Further preferably, the bobbin of the socket extension rod that described injector cartridge is formed is outer straight, and its inner core push rod end is connected with the block large with bobbin etc.
Further preferably, described inner core push rod end is blocked a shot and is provided with between barrel tip and can embed mutually and rotate the retaining ring fastened.
Second aspect of the present invention is to provide a kind of loading cryopreservation methods of biomaterial, adopts any one the miniature loading system described in the present invention first aspect, comprises the following steps:
Step 1, is connected taking out aerating device with the top end opening of cryovial, aspirating needle is run through elastic sealing element, is communicated with hollow structure and the external environment condition of cryovial;
Step 2, immerses the part being arranged in cryovial outside of aspirating needle and treats frozen liquid sample, use and take out aerating device, sucked by liquid sample in cryovial;
Step 3, first removes aspirating needle, then removes and takes out aerating device, is then inserted in cryovial by the top end opening of the bottom plug of socket extension rod from cryovial, obtains frozen unit; Or the aerating device of taking out of described syringe is injector cartridge, simultaneously directly to form again socket extension rod, dismounting aspirating needle, obtains frozen unit;
Step 4, by frozen for frozen unit, at least puts upside down once frozen unit before freezing, makes the gap be socketed between the bottom plug of extension rod and the top end opening of cryovial have liquid filling.
3rd aspect of the present invention is to provide thawing of a kind of biomaterial and unloads quadrat method, for adopt the loading cryopreservation methods of the present invention second aspect carry out frozen after thawing of frozen unit unload sample.
The thawing of described biomaterial unload that quadrat method adopts described in the present invention first aspect any one described in miniature loading system, comprise the following steps:
Step 1, thaws frozen unit;
Step 2, removes socket extension rod from cryovial; If the aerating device of taking out of described syringe is injector cartridge, simultaneously directly forms again and be socketed extension rod, then need not carry out this step;
Step 3, is connected taking out aerating device with the top end opening of cryovial, aspirating needle is run through elastic sealing element, is communicated with hollow structure and the external environment condition of cryovial;
Step 4, use is taken out aerating device and is extruded through aspirating needle by the liquid sample of cryovial.
The miniature loading system frozen for biomaterial provided by the invention guarantee freezing loading, mark, storage, resolution and taking-up and each operation such as to thaw more simple and convenient while, the space shared by carrier arrangement can also be reduced more significantly, and frozen safer.
Accompanying drawing explanation
Fig. 1 is the structural representation of the existing Load System for the biomaterial containing liquid;
Fig. 2 is the miniature loading system frozen for biomaterial provided by the invention and loads schematic flow sheet.
Embodiment
Referring to specific embodiment, the present invention is further illustrated, to understand the present invention better.
Embodiment 1
With reference to Fig. 1, the existing Load System for the biomaterial containing liquid comprises short cylindrical type cryopreservation tube 1 ', its bottom end closure, and top is passed through screw thread or click on fashion and block 2 ' and formed closed.This container is usually comparatively large, and volume generally more than 1 milliliter, and is stored on the layer-stepping carrier of liquid nitrogen container for box-packed year by grid mostly, also has the form of putting 3-4 pipe by the every bar cramp of aluminum clamping bar 3 ' to deposit.No matter it is grid box-like formula or aluminum clamping bar form, and the largest tube number that 50 liters of liquid nitrogen container can store is about 800 pipes.And the invention provides a kind of miniature loading system frozen for biomaterial, adopt cryovial 1 loading biological material of slim-lined construction, reduce the space shared by carrier arrangement significantly, and adopt socket to extend, 2 pairs of cryovials 1 carry out sealing simultaneously, also can be used for identifying, make freezing loading, mark, storage, resolution and taking-up and thawing etc. respectively operates more simple and convenient.
With reference to Fig. 2, the miniature loading system frozen for biomaterial provided by the invention comprises cryovial 1 and socket extension rod 2.
Cryovial 1 is hollow, and the bottom of cryovial is provided with the elastic sealing element 11 that rubber is made, and makes the bottom end seal of cryovial; The top end opening 12 of described cryovial.
Socket extension rod 2 is hollow tube, closed at both ends, and the bottom of socket extension rod 2 is provided with bottom plug 21.
The external diameter of socket extension rod 2 is identical with the external diameter of cryovial 1, and external diameter≤8.5mm.
The sidewall of the bottom of socket extension rod 2 is provided with liquid-through hole 22.
The socket top of extension rod 2 and/or the sidewall at top are provided with liquid-through hole 23.
Also comprise liquid sucking device, described liquid sucking device is syringe, and syringe comprises injector cartridge 3(and takes out aerating device) and syringe needle 4(aspirating needle).
When operating freezing, get a syringe, its syringe needle 4 is extracted elastic sealing element 11 to the syringe needle inserted bottom cryovial 1 and is exposed by sterile working, the plug of injector cartridge 3 is inserted the top end opening 12 of cryovial 1, syringe needle 4 other end is immersed and needs freezing liquid sample, twitch injector cartridge 3 and sample is drawn into height required in cryovial 1, extract syringe needle 4, then injector cartridge 3 is extracted, put socket extension rod 2 and fastening formation freezing unit, freezing unit is reversed, cryovial 1 is made to be stained with liquid with the junction of socket extension rod 2, then position proceeds frozen cooling operation routinely.When thawing, the freezing unit taken out from liquid nitrogen container is gone to the water bath containers of pre-temperature, hand-held socket extension rod 2 allows cryovial 1 have the part of freezing liquid to stir in warm water, and prevent water to be stained with cryovial 1 and the socket part being socketed extension rod 2, sample liquefaction to be frozen, clean periphery to wet water, unload socket extension rod 2, separately get a syringe, the plug of injector cartridge 3 is inserted long tube cryovial 1 upper end open by sterile working, then syringe needle 4 is inserted elastic sealing element 11 to the syringe needle of sterilizing cryovial 1 bottom to expose, the piston of pushing syringe cylindrical shell 3, Specimen liquid in cryovial is injected in sterile chamber through syringe needle 1.
Embodiment 2
The miniature loading system frozen for biomaterial that the present embodiment provides and the difference of embodiment 1 are, injector cartridge 3 directly forms socket extension rod 2.
In one preferred embodiment, the bobbin of the socket extension rod 2 that injector cartridge is formed is outer straight, and its inner core push rod end is connected with the block large with bobbin etc.
In further preferred embodiment, be provided with between described inner core push rod end block and barrel tip and can embed mutually and rotate the retaining ring fastened.
When operating freezing, get a syringe, its syringe needle 4 is extracted elastic sealing element 11 to the syringe needle inserted bottom cryovial 1 and is exposed by sterile working, the plug of injector cartridge 3 is inserted the top end opening 12 of cryovial 1, syringe needle 4 other end is immersed and needs freezing liquid sample, twitch injector cartridge 3 and sample is drawn into height required in cryovial 1, extract syringe needle 4, freezing unit, freezing unit is reversed, make cryovial 1 be stained with liquid with the junction of socket extension rod 2, then position proceeds frozen cooling operation routinely.When thawing, the freezing unit taken out from liquid nitrogen container is gone to the water bath containers of pre-temperature, hand syringes cylindrical shell 3 allows cryovial 1 have the part of freezing liquid to stir in warm water, and prevent water to be stained with cryovial 1 and the socket part being socketed extension rod 2, sample liquefaction to be frozen, clean periphery to wet water, then syringe needle 4 is inserted elastic sealing element 11 to the syringe needle of sterilizing cryovial 1 bottom to expose, the piston of pushing syringe cylindrical shell 3, is injected into the Specimen liquid in cryovial in sterile chamber through syringe needle 1.
Be described in detail specific embodiments of the invention above, but it is as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (12)
1. for the miniature loading system that biomaterial is frozen, it is characterized in that, comprise freezing unit, described freezing unit comprises cryovial and socket extension rod;
Described cryovial is that the bottom of hollow is provided with elastic sealing element, makes the bottom end seal of cryovial; The top end opening of described cryovial;
The bottom of described socket extension rod is provided with bottom plug, and described bottom plug is used for inserting in cryovial from the top end opening of cryovial forming sealing.
2. miniature loading system according to claim 1, is characterized in that, described socket extension rod is the tubular structure of all or part of hollow, closed at both ends.
3. miniature loading system according to claim 1, is characterized in that, the external diameter of described socket extension rod is identical with the external diameter of cryovial.
4. miniature loading system according to claim 2, is characterized in that, on the top of described socket extension rod and/or top sidewall and/or bottom sidewall be provided with liquid-through hole.
5. miniature loading system according to claim 1, is characterized in that, described elastic sealing element is made up of rubber.
6. miniature loading system according to claim 1, it is characterized in that, also comprise liquid sucking device, described liquid sucking device comprises takes out aerating device for what be tightly connected with the top end opening of cryovial, and for running through elastic sealing element thus the aspirating needle be communicated with external environment condition by the hollow structure of cryovial.
7. miniature loading system according to claim 6, is characterized in that, described liquid sucking device is syringe.
8. miniature loading system according to claim 7, is characterized in that, the aerating device of taking out of described syringe is injector cartridge, directly forms socket extension rod again simultaneously.
9. miniature loading system according to claim 8, is characterized in that, the bobbin of the socket extension rod that described injector cartridge is formed is outer straight, and its inner core push rod end is connected with the block large with bobbin etc.
10. miniature loading system according to claim 9, is characterized in that, is provided with and can embeds mutually and rotate the retaining ring fastened between described inner core push rod end block and barrel tip.
The loading cryopreservation methods of 11. 1 kinds of biomaterials, is characterized in that, adopts the miniature loading system described in any one in claim 1-10, comprises the following steps:
Step 1, is connected taking out aerating device with the top end opening of cryovial, aspirating needle is run through elastic sealing element, is communicated with hollow structure and the external environment condition of cryovial;
Step 2, immerses the part being arranged in cryovial outside of aspirating needle and treats frozen liquid sample, use and take out aerating device, sucked by liquid sample in cryovial;
Step 3, first removes aspirating needle, then removes and takes out aerating device, is then inserted in cryovial by the top end opening of the bottom plug of socket extension rod from cryovial, obtains frozen unit; Or the aerating device of taking out of described syringe is injector cartridge, simultaneously directly to form again socket extension rod, dismounting aspirating needle, obtains frozen unit;
Step 4, by frozen for frozen unit, at least puts upside down once frozen unit before freezing, makes the gap be socketed between the bottom plug of extension rod and the top end opening of cryovial have liquid filling.
Quadrat method is unloaded in thawing of 12. 1 kinds of biomaterials, it is characterized in that, to adopt loading cryopreservation methods according to claim 11 carry out frozen after frozen unit thaw and unload sample, comprise the following steps:
Step 1, thaws frozen unit;
Step 2, removes socket extension rod from cryovial; If the aerating device of taking out of described syringe is injector cartridge, simultaneously directly forms again and be socketed extension rod, then need not carry out this step;
Step 3, is connected taking out aerating device with the top end opening of cryovial, aspirating needle is run through elastic sealing element, is communicated with hollow structure and the external environment condition of cryovial;
Step 4, use is taken out aerating device and is extruded through aspirating needle by the liquid sample of cryovial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310594977.6A CN104642298A (en) | 2013-11-21 | 2013-11-21 | Miniature loading system for cryopreservation of biological materials |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310594977.6A CN104642298A (en) | 2013-11-21 | 2013-11-21 | Miniature loading system for cryopreservation of biological materials |
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| CN104642298A true CN104642298A (en) | 2015-05-27 |
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| CN201310594977.6A Pending CN104642298A (en) | 2013-11-21 | 2013-11-21 | Miniature loading system for cryopreservation of biological materials |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105923530A (en) * | 2016-07-11 | 2016-09-07 | 阳艳群 | Novel multilayer liquid nitrogen tank short bucket |
| CN112471136A (en) * | 2020-12-04 | 2021-03-12 | 深圳先进技术研究院 | Cell vitrification freezing treatment system and method |
| CN114403126A (en) * | 2021-12-06 | 2022-04-29 | 起源细胞技术(滁州)有限公司 | Closed embryo freezing tube and using method thereof |
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| CN102669088A (en) * | 2012-05-30 | 2012-09-19 | 河南省农业科学院 | Mammal embryo and oocyte vitrification freezing carrier |
| CN102742568A (en) * | 2011-04-18 | 2012-10-24 | 中国科学院过程工程研究所 | Apparatus for cryopreservation of biological sample |
| CN103238588A (en) * | 2013-05-21 | 2013-08-14 | 中国人民解放军第三军医大学第一附属医院 | Trace seminal fluid collecting, storing and freezing device |
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Patent Citations (6)
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| JP2001252293A (en) * | 2000-03-14 | 2001-09-18 | Hyogo Prefecture | Tool and method for vitrifying ovum or embryo |
| CN102405901A (en) * | 2010-09-21 | 2012-04-11 | 北京微创介入医疗装备有限公司 | Suction type glass vitrification freezing carrier device |
| CN102379278A (en) * | 2010-12-22 | 2012-03-21 | 车笠 | Cryogenic vial |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105923530A (en) * | 2016-07-11 | 2016-09-07 | 阳艳群 | Novel multilayer liquid nitrogen tank short bucket |
| CN112471136A (en) * | 2020-12-04 | 2021-03-12 | 深圳先进技术研究院 | Cell vitrification freezing treatment system and method |
| CN114403126A (en) * | 2021-12-06 | 2022-04-29 | 起源细胞技术(滁州)有限公司 | Closed embryo freezing tube and using method thereof |
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Application publication date: 20150527 |
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