CN203913154U - Carrying tool for use in vitrification cryo-preservation of biomaterial - Google Patents
Carrying tool for use in vitrification cryo-preservation of biomaterial Download PDFInfo
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- CN203913154U CN203913154U CN201420355515.9U CN201420355515U CN203913154U CN 203913154 U CN203913154 U CN 203913154U CN 201420355515 U CN201420355515 U CN 201420355515U CN 203913154 U CN203913154 U CN 203913154U
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- Prior art keywords
- hole
- vitrification
- preservation
- cryo
- counterweight
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- 239000012620 biological material Substances 0.000 title claims abstract description 38
- 238000004017 vitrification Methods 0.000 title claims abstract description 23
- 238000005138 cryopreservation Methods 0.000 title claims abstract description 20
- 238000007710 freezing Methods 0.000 claims abstract description 40
- 230000008014 freezing Effects 0.000 claims abstract description 40
- 238000007789 sealing Methods 0.000 claims abstract description 11
- 229920005644 polyethylene terephthalate glycol copolymer Polymers 0.000 claims abstract description 6
- 239000003086 colorant Substances 0.000 claims description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 abstract description 22
- 239000007788 liquid Substances 0.000 abstract description 13
- 229910052757 nitrogen Inorganic materials 0.000 abstract description 11
- 238000007667 floating Methods 0.000 abstract description 5
- 239000012472 biological sample Substances 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000005484 gravity Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 abstract description 2
- 241000700605 Viruses Species 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000010902 straw Substances 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 210000001161 mammalian embryo Anatomy 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 1
- 229920001634 Copolyester Polymers 0.000 description 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 1
- 239000005977 Ethylene Substances 0.000 description 1
- 102000029749 Microtubule Human genes 0.000 description 1
- 108091022875 Microtubule Proteins 0.000 description 1
- YIMQCDZDWXUDCA-UHFFFAOYSA-N [4-(hydroxymethyl)cyclohexyl]methanol Chemical compound OCC1CCC(CO)CC1 YIMQCDZDWXUDCA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 239000012520 frozen sample Substances 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 210000004688 microtubule Anatomy 0.000 description 1
- 210000000287 oocyte Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000000451 tissue damage Effects 0.000 description 1
- 231100000827 tissue damage Toxicity 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The utility model discloses a kind of carrying tool for use in vitrification cryo-preservation of biomaterial, it comprises inserted link main body, sheath and counterweight; Inserted link main body comprises and is provided with grafting body; Sheath is the PETG body of an end opening one end sealing, and it comprises cannula portion and be arranged on the freezing carrier of the sealing of the first end of cannula portion, and cannula portion is provided with through hole, and freezing carrier is provided with the cavity of containment of biological materials, and through hole is communicated with cavity; Counterweight is contained in through hole, and counterweight cover is pressed in the junction of through hole and cavity, grafting body and through hole are tightly connected, can make biomaterial be sealed in reliably in freezing carrier, complete and liquid nitrogen is isolated, avoid biological sample by the pollution of the virus and bacteria in liquid nitrogen and other etiologies, can make again biomaterial be difficult for losing.And under the effect of the gravity of counterweight, make the weight of inserted link main body and sheath increase, and there will not be floating phenomenon.In addition, counterweight also further seals freezing carrier, has improved sealing.
Description
Technical field
The utility model relates to biomedical sector, particularly a kind of carrying tool for use in vitrification cryo-preservation of biomaterial.
Background technology
Vitrification is the freezing carrying tool that utilizes volume small; extremely fast cooling (2000 DEG C/min of >) process in; the frozen solution that makes high concentration is changed into dense similar hyaloid solid-state by liquid state; avoid the formation of ice crystal in cell or tissue; reduce cell or tissue damage; reach cryoprotection cell or tissue, organize viability after freezing and the object of developmental potency thereby significantly improve cell or tissue.Thereby the application of vitrification in human oocyte and embryo's preservation is in recent years more and more extensive, is in vitro fertilization, microinjection, the important component part of transgenosis and clone embryos biotechnology.In addition, embryo vitrifying freeze technology can also be protected endangered animal, can make kind that value is high avoid suffering the potential threat of natural calamity, genetic drift, infectious disease.Vitrification does not need expensive programmed freezing instrument, only needs glass freezing to preserve carrying tool loss biomaterial easy and simple to handle and difficult.
Current existing vitrified frozen vector, as China Patent Publication No., CN10386814A discloses a kind of biological material vitrified frozen vector and preparation method thereof, and it comprises injection needle and oatmeal; Described injection needle is made up of syringe needle and needle holder; Described oatmeal is opening drawing-down straw, and its one end is sheathed in the inner chamber of described syringe needle; The straw wall disappearance of another free-end, the 1/5-1/3 that the cross section of remaining straw wall is circumference.Its weak point is to have potential biological pollution and carry the floating problem of bar.
Utility model content
In order to solve the problem of prior art, the utility model embodiment provides one not floating, and good airproof performance, free of contamination carrying tool for use in vitrification cryo-preservation of biomaterial.Described technical scheme is as follows:
In order to solve the problem of prior art, the utility model proposes following technical scheme:
A kind of carrying tool for use in vitrification cryo-preservation of biomaterial, it comprises inserted link main body, sheath and counterweight; Described inserted link main body comprises and is provided with grafting body; Described sheath is the PETG body of end opening one end sealing, it comprises cannula portion and is arranged on the freezing carrier of the sealing of the first end of described cannula portion, described cannula portion is provided with through hole, and described freezing carrier is provided with the cavity of containment of biological materials, and described through hole is communicated with described cavity; Described counterweight is contained in described through hole, and described counterweight cover is pressed in the junction of described through hole and described cavity; Described grafting body and described through hole are tightly connected.
Preferably, described through hole and described grafting body are connected by enjoyable.
Preferably, described grafting body is sphenoid, and described through hole is the bellmouth being connected with described grafting body.
Preferably, described grafting body is provided with external screw thread, and described through hole is internal thread hole, and described grafting body is connected with described through hole whorl.
Preferably, described inserted link main body also comprises handle, and described grafting body is extended and formed by described handle, and described handle is provided with skid-proof regions.
Preferably, the intersection of described handle and described grafting body is provided with step surface, described step surface can backstop in second end away from described freezing carrier of described cannula portion.
Preferably, the yi word pattern body of rod that described inserted link main body is multiple different colours.
Preferably, the inwall of described through hole is being provided with stop surface with described freezing carrier junction, and described stop surface backstop is in described counterweight
The beneficial effect that the technical scheme that the utility model provides is brought is:
Carrying tool for use in vitrification cryo-preservation of biomaterial of the present utility model is by close proximity between inserted link main body and sheath, can make biomaterial be sealed in reliably in freezing carrier, complete and liquid nitrogen is isolated, avoid biological sample by the pollution of the virus and bacteria in liquid nitrogen and other etiologies, can make biomaterial realize rapid glassization freezing, can make again biomaterial be difficult for losing.And under the effect of the gravity of counterweight, make the weight of inserted link main body and sheath increase, and there will not be floating phenomenon.In addition, counterweight also further seals freezing carrier, has improved sealing; And counterweight can make sheath in operating case in upright plumbness, the body of being convenient to peg graft inserts in cannula portion, simple in structure, easy to use.
Brief description of the drawings
Fig. 1 is the structural representation of facing of a kind of carrying tool for use in vitrification cryo-preservation of biomaterial of the utility model;
Fig. 2 is the inserted link agent structure schematic diagram of a kind of carrying tool for use in vitrification cryo-preservation of biomaterial of the utility model;
Wherein, Reference numeral:
1-inserted link main body, 2-sheath, 3-counterweight, the flat sheet freezing carrier of 4-, 11-handle, 12-grafting body.
Embodiment
For making the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing, the utility model embodiment is described in further detail.
Please refer to Fig. 1, the utility model embodiment provides a kind of carrying tool for use in vitrification cryo-preservation of biomaterial, and it comprises inserted link main body 1, is socketed on the sheath 2 of inserted link main body 1 and is placed in the counterweight 3 in sheath 2.
Incorporated by reference to reference to Fig. 2, the integrated straight-bar structure of inserted link main body 1, it comprises handle 11 and by the vertically extending grafting body 12 of handle 11.Wherein, the transverse width of handle 11 is greater than the transverse width of grafting body 12, thereby is formed with step surface 111 in handle 11 and the junction of grafting body 12.Step surface 111 can be for backstop sheath 2, prevents that inserted link main body 1 from all inserting in sheaths 2 and cannot extract.Preferably, handle 11 is provided with the skid-proof regions such as salient point, ripple to increase frictional force, convenient operation, and feel is good, and outward appearance is generous.
Please refer to Fig. 1, sheath 2 is made up of the macromolecular material of a kind of splendid thermal conductivity and surface tension characteristics, such as: the body that sheath 2 seals for end opening one end that PETG makes.The English name full name Poly (ethylene terephthalateco-1,4-cylclohexylenedimethyleneterephthalate) of PETG is a kind of transparent, non-crystalline type copolyesters; The comonomer that PETG is conventional is 1,4-CHDM (CHDM, Cylclohexylenedimethylene), and full name is PETG-1,4-CHDM ester.Sheath 2 comprises cannula portion 21 and is arranged on the freezing carrier 4 of the flat sheet of the self-enclosed structure of the first end of cannula portion 21.Cannula portion 21 comprises second end relative with first end, and cannula portion 21 is axially provided with the through hole (not label) that runs through its first end and the second end, thereby the second end of cannula portion 21 is openend.Grafting body 12 is plugged in the through hole of cannula portion 21 from the second end of cannula portion 21 by gap or enjoyable cooperation, thereby sheath 2 is closed.Grafting body 12 is plugged in the process of through hole, if while overexerting, the end face backstop of the second end of cannula portion 21 is in step surface 111, prevents that inserted link main body 1 from all inserting in sheaths 2 and cannot extract.
Please refer to Fig. 1, freezing carrier 4 is tapering point 41 towards the first end of cannula portion 21, and the vertex of a cone place of tapering point 41 is connected with the first end of cannula portion 21.In freezing carrier 4, be provided with the cavity (not label) for containment of biological materials.This cavity is communicated with the through hole of cannula portion 21, and the junction of the vertex of a cone of the inwall of the through hole of cannula portion 21 and tapering point 41 is formed with stop surface 411.This stop surface 411 is for backstop counterweight 3, pollutes biomaterial to avoid counterweight 3 to slip in the cavity of freezing carrier 4; And now counterweight 3 lids are pressed in the junction of described through hole and described cavity, and the cavity of freezing carrier 4 is further sealed.Preferably, in other embodiments, freezing carrier 4 can arrange tapering point 41, but is directly connected with cannula portion 21; Now, stop surface 411 is just arranged on the inwall of through hole and the junction of freezing carrier 4 of cannula portion 21.
While using the utility model carrying tool for use in vitrification cryo-preservation of biomaterial to carry out the freezing preservation of biomaterial (comprising cell or tissue), patient information and mark stick on the handle 11 of inserted link main body 1.Before glass freezing starts, sheath 2 is placed in to refrigeration operation box liquid nitrogen more than at least three ten seconds, and is fixed on the fixed mount of operating case.Microtubule is the second end from cannula portion 21 by the biomaterial such as ovum or blastular, and after the through hole of sheath 2, injects freezing carrier 4.Then fast counterweight 3 is placed in the through hole of sheath 2 from the second end of cannula portion 21, and grafting body 12 is coordinated to grafting with the through hole of sheath 2.Now, carry out fast glass freezing preservation, its biomaterial is contained in freezing carrier 4 and is not directly exposed in liquid nitrogen.Then take off the inserted link main body 1 and the sheath 2 that are fixed on fixed mount, and they are transferred in existing cryovial in liquid nitrogen, to preserve for a long time in liquid nitrogen container.In this process, by the socket connection between inserted link main body 1 and sheath 2, the freezing carrier 4 of carrying biomaterial is sealed reliably, completely next door liquid nitrogen, avoided biomaterial in freezing carrier 4, to reveal and contact with liquid nitrogen and pollution to biomaterial.And under the effect of the gravity of counterweight 3, make inserted link main body 1 and the weight of sheath 2 increase, and there will not be floating phenomenon.In addition, counterweight 3 also further seals freezing carrier 4, has improved sealing; And counterweight 3 can make sheath 2 in operating case in upright plumbness, the body 12 of being convenient to peg graft inserts in cannula portion 21.
Particularly, in the present embodiment, for convenient, handle 11 is provided with pastes district, is specifically designed to binding mark numbering and patient information.More preferably, the yi word pattern body of rod that inserted link main body 1 can adopt the material of different colours to make, is convenient to distinguish.
Particularly, in the present embodiment, for the body 12 that prevents from pegging graft departs from from cannula portion 21, grafting body 12 is provided with external screw thread, and the through-hole wall at the first end place of cannula portion 21 is provided with the internal thread with body 12 corresponding matching of pegging graft; Like this, grafting body 12 is threaded with cannula portion 21.More preferably, grafting body 12 is sphenoid, and the through hole at the first end place of cannula portion 21 is the bellmouth coordinating with grafting body 12, and like this, grafting body 12 is connected for shrinking taper type with cannula portion 21.
Particularly, in the present embodiment, the self-enclosed structure of segment that freezing carrier 4 is formed through extrusion processing by the second end of the cannula portion 21 of sheath 2.
The utility model carrying tool for use in vitrification cryo-preservation of biomaterial is rational in infrastructure, combine easy, sealing is reliable, processing technology is superior, possesses the hommization of height, can isolate liquid nitrogen completely, avoid potential biological pollution danger, realize biological sample rapid glassization freezing, it is easy for operation, sample storeroom is through carrier and the design of liquid surface hydrodynamics accurately, have or not use experience, will not lose and be frozen sample, new hand can obtain the same freezing and defrost result with skilled worker, frozen efficiency is high, result of use is good, biological sample recovery survival rate is high, and can directly the jerrycan of sealing be made in sheath one end or middle part simultaneously, form multiple flat sheet freezing carriers of placing ovum or blastular, be equal to the simultaneously freezing effect of multitube, therefore cost performance is high.
The foregoing is only preferred embodiment of the present utility model, not in order to limit the utility model, all within spirit of the present utility model and principle, any amendment of doing, be equal to replacement, improvement etc., within all should being included in protection domain of the present utility model.
Claims (8)
1. a carrying tool for use in vitrification cryo-preservation of biomaterial, is characterized in that, it comprises inserted link main body, sheath and counterweight;
Described inserted link main body comprises and is provided with grafting body;
Described sheath is the PETG body of end opening one end sealing, it comprises cannula portion and is arranged on the freezing carrier of the sealing of the first end of described cannula portion, described cannula portion is provided with through hole, and described freezing carrier is provided with the cavity of containment of biological materials, and described through hole is communicated with described cavity;
Described counterweight is contained in described through hole, and described counterweight cover is pressed in the junction of described through hole and described cavity; Described grafting body and described through hole are tightly connected.
2. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 1, is characterized in that, described through hole and described grafting body are connected by enjoyable.
3. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 1, is characterized in that, described grafting body is sphenoid, and described through hole is the bellmouth being connected with described grafting body.
4. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 1, is characterized in that, described grafting body is provided with external screw thread, and described through hole is internal thread hole, and described grafting body is connected with described through hole whorl.
5. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 1, is characterized in that, described inserted link main body also comprises handle, and described grafting body is extended and formed by described handle, and described handle is provided with skid-proof regions.
6. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 5, is characterized in that, the intersection of described handle and described grafting body is provided with step surface, described step surface can backstop in second end away from described freezing carrier of described cannula portion.
7. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 1, is characterized in that, the yi word pattern body of rod that described inserted link main body is multiple different colours.
8. carrying tool for use in vitrification cryo-preservation of biomaterial according to claim 1, is characterized in that, the inwall of described through hole is being provided with stop surface with described freezing carrier junction, and described stop surface backstop is in described counterweight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420355515.9U CN203913154U (en) | 2014-06-27 | 2014-06-27 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420355515.9U CN203913154U (en) | 2014-06-27 | 2014-06-27 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
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| Publication Number | Publication Date |
|---|---|
| CN203913154U true CN203913154U (en) | 2014-11-05 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420355515.9U Expired - Fee Related CN203913154U (en) | 2014-06-27 | 2014-06-27 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
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| CN (1) | CN203913154U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660605A (en) * | 2016-03-09 | 2016-06-15 | 柳州市妇幼保健院 | Efficient gamete/embryo vitrification thawing method |
| CN107836446A (en) * | 2017-10-31 | 2018-03-27 | 江西易通医疗器械有限公司 | Freezing tube |
| CN108541702A (en) * | 2018-05-30 | 2018-09-18 | 湖北华美阳光生物科技有限公司 | A kind of vitreous with coding information chip freezes bar |
| CN110637809A (en) * | 2019-10-10 | 2020-01-03 | 广州辅康生物科技有限公司 | Carrier Sleeve |
| WO2021042587A1 (en) * | 2019-09-06 | 2021-03-11 | 苏州贝康医疗器械有限公司 | Vitrification freezing carrier |
-
2014
- 2014-06-27 CN CN201420355515.9U patent/CN203913154U/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105660605A (en) * | 2016-03-09 | 2016-06-15 | 柳州市妇幼保健院 | Efficient gamete/embryo vitrification thawing method |
| CN105660605B (en) * | 2016-03-09 | 2018-07-31 | 柳州市妇幼保健院 | A kind of efficient gamete/freeze-thaw method for vitrification of embryo |
| CN107836446A (en) * | 2017-10-31 | 2018-03-27 | 江西易通医疗器械有限公司 | Freezing tube |
| CN108541702A (en) * | 2018-05-30 | 2018-09-18 | 湖北华美阳光生物科技有限公司 | A kind of vitreous with coding information chip freezes bar |
| WO2021042587A1 (en) * | 2019-09-06 | 2021-03-11 | 苏州贝康医疗器械有限公司 | Vitrification freezing carrier |
| EP4026426A4 (en) * | 2019-09-06 | 2023-10-25 | Suzhou Basecare Medical Device Co., Ltd. | GLAZING FREEZING CARRIER |
| CN110637809A (en) * | 2019-10-10 | 2020-01-03 | 广州辅康生物科技有限公司 | Carrier Sleeve |
| CN110637809B (en) * | 2019-10-10 | 2024-02-23 | 广州辅康生物科技有限公司 | Support rod sleeve |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141105 Termination date: 20160627 |