CN104719283A - Closed freezing carrier and use method thereof - Google Patents
Closed freezing carrier and use method thereof Download PDFInfo
- Publication number
- CN104719283A CN104719283A CN201510130076.0A CN201510130076A CN104719283A CN 104719283 A CN104719283 A CN 104719283A CN 201510130076 A CN201510130076 A CN 201510130076A CN 104719283 A CN104719283 A CN 104719283A
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- freezing
- closed
- sleeve pipe
- liquid nitrogen
- liquid
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Abstract
The invention discloses a closed freezing carrier and a use method thereof. The closed freezing carrier is characterized in that a connecting part is sleeved with at least one liquid seal ring; a casing is in a one-end opened and one-end closed pipe structure; the connecting part and a freezing storage part enter the casing through an opening of the casing and are arranged in the casing in a sleeving manner; the liquid seal ring is clamped at an opening end of the casing; the liquid seal ring, the connecting part, the freezing storage part and a bottom space of the casing pie form a closed freezing space; and liquid adhered to the liquid seal ring fills into all gaps of a joint between the liquid seal ring and the casing, and is frozen and then solidified, so that the closed freezing space is sealed. The closed freezing carrier allows that a biological sample does not contact with ordinary liquid nitrogen with potential organism hazards during frozen storage; various operations such as freezing, loading, marking, storage, resolution, taking out and thawing are simple, convenient and quick; in addition, the space occupied by a carrier device can be reduced safer and more remarkably; and the storage quantity of each liquid nitrogen container is increased by about 3-8 times.
Description
Technical field
The present invention relates to a kind of carrier frozen for biomaterial, in particular a kind of closed freezing carrier and using method thereof.
Background technology
At present, in the vitrified frozen vector used when supplementary reproduction medical domain carries out the glass freezing of human embryos or ovum, known embryo vitrification freezing carrier has many kinds, wherein open frozen, namely embryo directly contacts the frozen of liquid nitrogen, becomes the optimised form of embryo vitrifying freeze because cooling is fast.Its carrier usually adopted is the freezing rod type that front end has frozen carrier sheet (being called for short frozen), and it is simple to operate fast and efficient.
But there is following problem in current embryo vitrifying freeze bar and corresponding Load System thereof:
First, this freezing be open freezing, between biomaterial with liquid nitrogen exist contact.This just cannot be avoided microorganism in common liquid nitrogen to be infected with the potential of frozen material, and chemical composition also may to be caused in liquid nitrogen the harm of biomaterial;
Secondly, storage area application efficiency is low.This reason is wherein that conventional carriers system bulk is large, and is that multiple years bars are put into one together again and escorted cylinder (as conventional embryo vitrifying freeze carry bar tub), which increases the space of escorting shared by cylinder; Simultaneously to escort in cylinder before little freezing unit has not successively all thawed at one, can not add new freezing unit at any time it in, otherwise can cause and store confusion, Gu Qisuo takes up space can not with to thaw and release new space; And multiple escort that cylinder is removed thaw in a large number time, surplus have the cylinder that escorts of little freezing unit to be returned to each well-bucket position of each liquid nitrogen container of original place.This return process easily occurs that position is returned and makes mistakes, for searching of thawing next time brings difficulty.
Summary of the invention
The object of the invention is to overcome the deficiencies in the prior art, provide a kind of closed freezing carrier and using method thereof, adopt tight closure, that the design of microminiaturization and single pole realizes operating is safe and reliable.
The present invention is achieved by the following technical solutions, the present invention includes and carry bar and sleeve pipe, within described year, bar comprises the bar handle from top to bottom connected successively, connecting portion and frozen portion, at least one fluid-tight circle sheathed on described connecting portion, sleeve pipe is the tubular structure that one end open one end is closed, described connecting portion and frozen portion are entered by the opening of sleeve pipe and are set in sleeve pipe, fluid-tight circle is fastened on the openend of sleeve pipe, fluid-tight circle, connecting portion, the reefer space that between frozen portion and the inwall of sleeve pipe, formation one is closed, stick to all gaps of liquid filling fluid-tight circle on fluid-tight circle and sleeve pipe joint, liquid is through freezing the sealing of after coagulation realization to described closed reefer space.
As one of preferred embodiment of the present invention, on described connecting portion sheathed at least one except liquid circle, to be describedly positioned under fluid-tight circle except liquid circle.
As one of preferred embodiment of the present invention, the described liquid absorbent material be containing pore or lyophily structure except liquid circle and fluid-tight circle is made, and except liquid circle is near frozen portion, fluid-tight circle is near bar handle.
Outer sleeve is the tubular structure that one end open one end is closed, and the sidewall of outer sleeve offers connected entrance, and described sleeve pipe is entered by the opening of outer sleeve and is set in outer sleeve.
As one of preferred embodiment of the present invention, the outer, top of described sleeve pipe is provided with Coupling, and described Coupling is fastened on the opening of outer sleeve.
As one of preferred embodiment of the present invention, the bottom of described outer sleeve is provided with metal pouring weight.
As one of preferred embodiment of the present invention, the top of described bar handle is provided with index plane, and the external diameter of bar handle is more than or equal to 2.3mm.
As one of preferred embodiment of the present invention, described frozen portion is thin slice; Described thin slice is the groove structure of both sides projection medial recess.
A using method for closed freezing carrier, comprise refrigeration operation and operation of thawing, described refrigeration operation comprises the following steps:
(1) clean for ultralow temperature freezing put into the box body that common liquid nitrogen is housed and avoid common liquid nitrogen to enter clean freezing of ultralow temperature;
(2) in bar handle and the enterprising row labels of index plane;
(3) fluid-tight circle is allowed to adsorb upper liquid and make it be in non-frozen state, the biomaterial that will preserve is placed in frozen portion, frozen portion is inserted in clean freezing of ultralow temperature freezing, under sleeve pipe being placed in condition of ultralow temperature with disinfecting forceps, but the openend of retaining sleeve is in outside liquid nitrogen always, the opening of rapid for frozen portion alignment sleeve is inserted, and the fluid-tight circle on connecting portion and sleeve pipe are locked, form the reefer space closed, then in liquid nitrogen, sleeve pipe is inserted into outer sleeve internal fixtion, or in advance sleeve pipe is inserted in outer sleeve, finally the closed freezing carrier assembled is transferred to the liquid nitrogen container that common liquid nitrogen is housed frozen,
Described operating procedure of thawing is as follows:
(4) common liquid nitrogen is provided to be loaded in cylinder or box type container, by the freezing position of the closed freezing carrier of record search, after cross-check information, rapidly its middle and lower part is immersed in the liquid nitrogen of container, the opening of sleeve pipe is positioned at outside liquid nitrogen surface, and in sleeve pipe, present position, frozen portion is under liquid nitrogen surface;
(5) one holding rod handles, the opening portion of exposing of outer sleeve or sleeve pipe clamped by another hand tweezers, extracts rapidly and carry bar outside liquid nitrogen surface, and then rapid being inserted in frozen portion is thawed in operation liquid, starts to thaw by thaw routine.
Described freezing of ultralow temperature cleaning is the aseptic filtration liquid nitrogen of sterile chamber and splendid attire thereof, or is in the sterilizing firmware inner space of liquid nitrogen encirclement, or the steam state space of liquid nitrogen volatilization.
The present invention has the following advantages compared to existing technology: the present invention makes biological specimen when frozen, not contact the common liquid nitrogen having potential harms of microbe, guarantee freezing loading, mark, storage, resolution and taking-up and to thaw etc. each simple and convenient while, can also be safer, and the space of reducing more significantly shared by carrier arrangement, make each liquid nitrogen container amount of storage increase about 3-8 doubly.
Accompanying drawing explanation
Fig. 1 is the structural representation that bar is carried in the present invention;
Fig. 2 is the structural representation of sleeve pipe;
Fig. 3 is the structural representation of outer sleeve;
Fig. 4 is the structural representation after the present invention assembles.
Embodiment
Elaborate to embodiments of the invention below, the present embodiment is implemented under premised on technical solution of the present invention, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.
As shown in figures 1-4, the present embodiment comprises a year bar 1, sleeve pipe 3 and outer sleeve 5, within described year, bar 1 comprises the bar handle 11 from top to bottom connected successively, connecting portion 12 and frozen portion 13, a sheathed fluid-tight circle 2 on described connecting portion 12, the tubular structure that sleeve pipe 3 is closed for one end open one end, described connecting portion 12 and frozen portion 13 are entered by the opening of sleeve pipe 3 and are set in sleeve pipe 3, fluid-tight circle 2 is fastened on the openend of sleeve pipe 3, fluid-tight circle 2, connecting portion 12, the reefer space that between frozen portion 13 and the inwall of sleeve pipe 3, formation one is closed, the tubular structure that outer sleeve 5 is closed for one end open one end, the sidewall of outer sleeve 5 is offered connected entrance 51, described sleeve pipe 3 is entered by the opening of outer sleeve 5 and is set in outer sleeve 5, on connecting portion 12, sheathed one is removed liquid circle 4, the described liquid circle 4 that removes is positioned under fluid-tight circle 2.
When closing frozen, fluid-tight circle 2 surrounding is loaded liquid, then frozen portion 13, connecting portion 12 and fluid-tight circle 2 are inserted in sleeve pipe 3, stick to all gaps of liquid filling fluid-tight circle on fluid-tight circle 22 and sleeve pipe 3 joint, the sealing that liquid realizes described closed reefer space through freezing after coagulation.
Except liquid circle 4 keeps dry in frozen process, when thawing, in the process that sleeve pipe 3 is drawn out, except liquid circle 4 can will be attached to the residual liquid Adsorption of sleeve pipe 3 tube wall through fluid-tight circle 2 original position, ensure that the frozen portion 13 of its underpart is not subject to residual liquid and is infected with being drawn out in process.
The liquid absorbent material that liquid circle and fluid-tight circle 2 are containing pore or lyophily structure is made, except liquid circle 4 is near frozen portion 13, fluid-tight circle 2 near bar handle 11, the present embodiment adopt lyophilic material such as cotton, sponge etc. to make except liquid circle 4 and fluid-tight circle 2.
The outer, top of sleeve pipe 3 is provided with Coupling 31, and described Coupling 31 is fastened on the opening of outer sleeve 5.The materials such as Coupling 31 is rubber are made, or are the expand structure of sleeve pipe 3 at outer, top place, can guarantee that sleeve pipe 3 is fixed on outer sleeve 5.
The bottom of outer sleeve 5 is provided with metal pouring weight 52, can increase overall weight.
For the ease of marker recognition, the top of bar handle 11 is provided with index plane 14, and the external diameter of bar handle 11 is more than or equal to 2.3mm.
Frozen portion 13 is thin slice, and thin slice is the groove structure of both sides projection medial recess, for preserving the placement position of biomaterial in groove structure.
A using method for closed freezing carrier, comprise refrigeration operation and operation of thawing, described refrigeration operation comprises the following steps:
(1) clean for ultralow temperature freezing put into the box body that common liquid nitrogen is housed and avoid common liquid nitrogen to enter clean freezing of ultralow temperature; Freezing of ultralow temperature cleaning is the aseptic filtration liquid nitrogen of sterile chamber and splendid attire thereof;
(2) in bar handle 11 and the enterprising row labels of index plane 14;
(3) fluid-tight circle 2 is allowed to adsorb upper liquid and make it be in non-frozen state, the biomaterial that will preserve is placed in frozen portion 13, frozen portion 13 is inserted in clean freezing of ultralow temperature freezing, under sleeve pipe 3 being placed in condition of ultralow temperature with disinfecting forceps, but the openend of retaining sleeve 3 is in outside liquid nitrogen always, the opening of rapid for frozen portion 13 alignment sleeve 3 is inserted, and the fluid-tight circle 2 on connecting portion 12 is locked with sleeve pipe 3, form the reefer space closed, then in liquid nitrogen, sleeve pipe 3 is inserted into outer sleeve 5 internal fixtion, or in advance sleeve pipe 3 is inserted in outer sleeve 5, finally the closed freezing carrier assembled is transferred to the liquid nitrogen container that common liquid nitrogen is housed frozen,
Described operating procedure of thawing is as follows:
(4) common liquid nitrogen is provided to be loaded in cylinder or box type container, by the freezing position of the closed freezing carrier of record search, after cross-check information, rapidly its middle and lower part is immersed in the liquid nitrogen of container, the opening of sleeve pipe 3 is positioned at outside liquid nitrogen surface, and in sleeve pipe 3, present position, frozen portion 13 is under liquid nitrogen surface;
(5) one holding rod handles 11, the opening portion of exposing of outer sleeve 5 or sleeve pipe 3 clamped by another hand tweezers, extracts rapidly and carry bar 1 outside liquid nitrogen surface, and then rapid being inserted in frozen portion 13 is thawed in operation liquid, starts to thaw by thaw routine.
Claims (10)
1. a closed freezing carrier, it is characterized in that, comprise and carry bar (1) and sleeve pipe (3), described year bar (1) comprise the bar handle (11) from top to bottom connected successively, connecting portion (12) and frozen portion (13), upper at least one the fluid-tight circle (2) sheathed of described connecting portion (12), the tubular structure that sleeve pipe (3) is closed for one end open one end, described connecting portion (12) and frozen portion (13) are entered by the opening of sleeve pipe (3) and are set in sleeve pipe (3), fluid-tight circle (2) is fastened on the openend of sleeve pipe (3), fluid-tight circle (2), connecting portion (12), the reefer space that between the inwall of frozen portion (13) and sleeve pipe (3), formation one is closed, stick to all gaps of liquid filling fluid-tight circle (2) on fluid-tight circle (2) and sleeve pipe (3) joint, liquid is through freezing the sealing of after coagulation realization to described closed reefer space.
2. the closed freezing carrier of one according to claim 1, is characterized in that, described connecting portion (12) upper sheathed at least one except liquid circle (4), to be describedly positioned under fluid-tight circle (2) except liquid circle (4).
3. the closed freezing carrier of one according to claim 2, it is characterized in that, the described liquid absorbent material be containing pore or lyophily structure except liquid circle (4) and fluid-tight circle (2) is made, except liquid circle (4) is near frozen portion (13), fluid-tight circle (2) is near bar handle (11).
4. the closed freezing carrier of one according to claim 1, it is characterized in that, described carrier also comprises outer sleeve (5), the tubular structure that outer sleeve (5) is closed for one end open one end, the sidewall of outer sleeve (5) is offered connected entrance (51), described sleeve pipe (3) is entered by the opening of outer sleeve (5) and is set in outer sleeve (5).
5. the closed freezing carrier of one according to claim 4, it is characterized in that, the outer, top of described sleeve pipe (3) is provided with Coupling (31), and described Coupling (31) is fastened on the opening of outer sleeve (5).
6. the closed freezing carrier of one according to claim 4, is characterized in that, the bottom of described outer sleeve (5) is provided with metal pouring weight (52).
7. the closed freezing carrier of one according to claim 1, is characterized in that, the top of described bar handle (11) is provided with index plane (14), and the external diameter of bar handle (11) is more than or equal to 2.3mm.
8. the closed freezing carrier of one according to claim 1, is characterized in that, described frozen portion (13) is thin slice; Described thin slice is the groove structure of both sides projection medial recess.
9. a using method for the closed freezing carrier as described in any one of claim 1 ~ 8, is characterized in that, comprise refrigeration operation and operation of thawing, described refrigeration operation comprises the following steps:
(1) clean for ultralow temperature freezing put into the box body that common liquid nitrogen is housed and avoid common liquid nitrogen to enter clean freezing of ultralow temperature;
(2) in bar handle (11) and index plane (14) enterprising row labels;
(3) liquid in fluid-tight circle (2) absorption make it be in non-frozen state is allowed, the biomaterial that will preserve is placed in frozen portion (13), frozen portion (13) are inserted in clean freezing of ultralow temperature freezing, under sleeve pipe (3) being placed in condition of ultralow temperature with disinfecting forceps, but the openend of retaining sleeve (3) is in outside liquid nitrogen always, the opening of rapid to frozen portion (13) alignment sleeve (3) is inserted, and the fluid-tight circle (2) on connecting portion (12) and sleeve pipe (3) are locked, form the reefer space closed, then in liquid nitrogen, sleeve pipe (3) is inserted into outer sleeve (5) internal fixtion, or in advance sleeve pipe (3) is inserted in outer sleeve (5), finally the closed freezing carrier assembled is transferred to the liquid nitrogen container that common liquid nitrogen is housed frozen,
Described operating procedure of thawing is as follows:
(4) common liquid nitrogen is provided to be loaded in cylinder or box type container, by the freezing position of the closed freezing carrier of record search, after cross-check information, rapidly its middle and lower part is immersed in the liquid nitrogen of container, the opening of sleeve pipe (3) is positioned at outside liquid nitrogen surface, and in sleeve pipe (3), frozen portion (13) present position is under liquid nitrogen surface;
(5) one holding rod handles (11), the opening portion of exposing of outer sleeve (5) or sleeve pipe (3) clamped by another hand tweezers, extract outside liquid nitrogen surface rapidly and carry bar (1), then rapid frozen portion (13) being inserted into is thawed in operation liquid, starts to thaw by thaw routine.
10. the using method of a kind of closed freezing carrier as claimed in claim 9, it is characterized in that, described freezing of ultralow temperature cleaning is the aseptic filtration liquid nitrogen of sterile chamber and splendid attire thereof, or is in the sterilizing firmware inner space of liquid nitrogen encirclement, or the steam state space of liquid nitrogen volatilization.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510130076.0A CN104719283A (en) | 2015-03-24 | 2015-03-24 | Closed freezing carrier and use method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510130076.0A CN104719283A (en) | 2015-03-24 | 2015-03-24 | Closed freezing carrier and use method thereof |
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| Publication Number | Publication Date |
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| CN104719283A true CN104719283A (en) | 2015-06-24 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510130076.0A Pending CN104719283A (en) | 2015-03-24 | 2015-03-24 | Closed freezing carrier and use method thereof |
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| CN (1) | CN104719283A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489914A (en) * | 2016-10-20 | 2017-03-15 | 北京中仪康卫医疗器械有限公司 | A kind of freezing carries bar |
| CN107372470A (en) * | 2017-09-11 | 2017-11-24 | 马博 | A kind of ovum embryo vitrifying freeze save set |
| CN108617641A (en) * | 2018-07-03 | 2018-10-09 | 浙江星博生物科技股份有限公司 | A kind of biological sample glass frozen cryopreservation device |
| CN110637809A (en) * | 2019-10-10 | 2020-01-03 | 广州辅康生物科技有限公司 | Loading rod sleeve |
| WO2020078159A1 (en) * | 2018-10-17 | 2020-04-23 | 广州品知医疗器械有限公司 | Sealed vitrified refrigerating carrier |
| WO2020147604A1 (en) * | 2019-01-18 | 2020-07-23 | 上海廪典实业有限公司 | Ultralow-temperature freezing and sealing type preservation method |
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| CN102204527A (en) * | 2011-04-15 | 2011-10-05 | 山东威高新生医疗器械有限公司 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
| CN103444699A (en) * | 2013-08-01 | 2013-12-18 | 吕祁峰 | Miniature carrier system for cryopreservation of biological materials, bucket and cryopreservation method |
| CN203555094U (en) * | 2013-11-21 | 2014-04-23 | 上海交通大学医学院附属第九人民医院 | Loading rod system for sealing-type cryopreservation of biological materials |
| CN203618631U (en) * | 2013-11-21 | 2014-06-04 | 上海交通大学医学院附属第九人民医院 | Vitrification carrier freezing system |
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2015
- 2015-03-24 CN CN201510130076.0A patent/CN104719283A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102204527A (en) * | 2011-04-15 | 2011-10-05 | 山东威高新生医疗器械有限公司 | Carrying tool for use in vitrification cryo-preservation of biomaterial |
| CN103444699A (en) * | 2013-08-01 | 2013-12-18 | 吕祁峰 | Miniature carrier system for cryopreservation of biological materials, bucket and cryopreservation method |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106489914A (en) * | 2016-10-20 | 2017-03-15 | 北京中仪康卫医疗器械有限公司 | A kind of freezing carries bar |
| CN107372470A (en) * | 2017-09-11 | 2017-11-24 | 马博 | A kind of ovum embryo vitrifying freeze save set |
| CN107372470B (en) * | 2017-09-11 | 2021-05-28 | 马博 | Ovum embryo vitrification cryopreservation device |
| CN108617641A (en) * | 2018-07-03 | 2018-10-09 | 浙江星博生物科技股份有限公司 | A kind of biological sample glass frozen cryopreservation device |
| WO2020078159A1 (en) * | 2018-10-17 | 2020-04-23 | 广州品知医疗器械有限公司 | Sealed vitrified refrigerating carrier |
| WO2020147604A1 (en) * | 2019-01-18 | 2020-07-23 | 上海廪典实业有限公司 | Ultralow-temperature freezing and sealing type preservation method |
| CN110637809A (en) * | 2019-10-10 | 2020-01-03 | 广州辅康生物科技有限公司 | Loading rod sleeve |
| CN110637809B (en) * | 2019-10-10 | 2024-02-23 | 广州辅康生物科技有限公司 | Support rod sleeve |
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Application publication date: 20150624 |
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