CN108617641A - A kind of biological sample glass frozen cryopreservation device - Google Patents
A kind of biological sample glass frozen cryopreservation device Download PDFInfo
- Publication number
- CN108617641A CN108617641A CN201810722563.XA CN201810722563A CN108617641A CN 108617641 A CN108617641 A CN 108617641A CN 201810722563 A CN201810722563 A CN 201810722563A CN 108617641 A CN108617641 A CN 108617641A
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- Prior art keywords
- biological sample
- sample
- cryopreservation
- bar
- protective case
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- 238000005138 cryopreservation Methods 0.000 title claims abstract description 82
- 239000012472 biological sample Substances 0.000 title claims abstract description 49
- 239000011521 glass Substances 0.000 title claims abstract description 30
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 80
- 239000000523 sample Substances 0.000 claims abstract description 68
- 239000007788 liquid Substances 0.000 claims abstract description 44
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 40
- 230000007246 mechanism Effects 0.000 claims abstract description 35
- 230000001681 protective effect Effects 0.000 claims description 42
- 238000007710 freezing Methods 0.000 claims description 33
- 230000008014 freezing Effects 0.000 claims description 33
- 241000446313 Lamella Species 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims description 5
- 230000004044 response Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 4
- MTAZNLWOLGHBHU-UHFFFAOYSA-N butadiene-styrene rubber Chemical compound C=CC=C.C=CC1=CC=CC=C1 MTAZNLWOLGHBHU-UHFFFAOYSA-N 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 239000004743 Polypropylene Substances 0.000 claims description 2
- -1 polypropylene Polymers 0.000 claims description 2
- 229920001155 polypropylene Polymers 0.000 claims description 2
- 210000001161 mammalian embryo Anatomy 0.000 abstract description 8
- 239000011505 plaster Substances 0.000 abstract description 3
- 238000010586 diagram Methods 0.000 description 4
- 239000010902 straw Substances 0.000 description 3
- 238000012790 confirmation Methods 0.000 description 2
- 238000012864 cross contamination Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 235000015110 jellies Nutrition 0.000 description 2
- 239000008274 jelly Substances 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 238000010257 thawing Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 150000001336 alkenes Chemical class 0.000 description 1
- 208000002925 dental caries Diseases 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000021267 infertility disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- QQONPFPTGQHPMA-UHFFFAOYSA-N propylene Natural products CC=C QQONPFPTGQHPMA-UHFFFAOYSA-N 0.000 description 1
- 125000004805 propylene group Chemical group [H]C([H])([H])C([H])([*:1])C([H])([H])[*:2] 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
- A01N1/0268—Carriers for immersion in cryogenic fluid, both for slow-freezing and vitrification, e.g. open or closed "straws" for embryos, oocytes or semen
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Mechanical Engineering (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention relates to a kind of biological sample glass frozen cryopreservation devices, including sample loader mechanism and holding structure, the sample loader mechanism is for loading biological sample, for storing the more pieces of sample loader mechanisms, multiple holding structures overlay in liquid nitrogen container to increase the amount of storage of biological sample the holding structure.Biological sample save set of the present invention solves existing cryopreservation device due to being all slender type sticking plaster, can only store one layer of sample, thus a large amount of the problem of occupying liquid nitrogen container parking space, and it is easy to operate, sample can be positioned rapidly, prevents embryo from obscuring, and reduce pollution risk.
Description
Technical field
The present invention relates to a kind of biological sample save set, especially a kind of biological sample glass frozen cryopreservation device.
Background technology
Be used for preserving frozen embryo or egg mother cell currently, in supplementary reproduction field, in laboratory predominantly freezes load
Bar and freeze straw.As described in Chinese patent literature CN 106489914A, freezing carries the modeling that bar is non-isodiametric elongated shape
Charge bar, taper end are used for sample loading, tubbiness is handheld terminal close to lamella tip.Straw is the hollow plastic stick of strip.One end
Connection loads the load bar of sample, and the other end is the veil containing cotton thread of filtration, and prevents the steel loop floated in liquid nitrogen.It presses
Such mode, one layer of sample can only be stored since the two is all slender type sticking plaster by carrying bar cooperation freezing straw using freezing,
Occupy larger space in liquid nitrogen container.With the increase of infertile crowd in recent years, receive the patient of embryo transfer also increasingly
It is more, it is annual newly-increased to freeze embryo's quantity and also increase.Many reproductive centers increase more than ten or even tens liquid nitrogen containers newly every year, with full
The growing embryo freezing of foot needs.
In addition, the existing load bar that freezes is in semi-closed state, there are the risks of sample cross contamination.Meanwhile defrosting sample
When product, due to the influence of steam above liquid nitrogen, bar rack surface frosting is carried, the label above holder is covered, can not be carried from one
Bucket quickly finds sample, extends the artificial time for finding sample.
Invention content
The present invention devises a kind of biological sample glass frozen cryopreservation device, and the technical issues of solving is existing to freeze dress
It sets due to being all slender type sticking plaster, one layer of sample, thus a large amount of parking spaces for occupying liquid nitrogen container can only be stored.
In order to solve above-mentioned technical problem, present invention employs following scheme：
A kind of biological sample glass frozen cryopreservation device, including sample loader mechanism and holding structure, the sample loading machine
Structure is for loading biological sample, and the holding structure is for storing the more pieces of sample loader mechanisms, multiple holding structures
It overlays in the liquid nitrogen container to increase the amount of storage of biological sample.
Further, the holding structure includes freezing storing box (7) and box cover (8), the box cover (8) and the freezing storing box (7)
With the form matches of paper box, the freezing storing box (7) is provided with multiple hole slots (9) for fixed sample loader mechanism, described
The size of hole slot (9) matches with the sample loader mechanism, and the side of the sample loader mechanism is provided with multiple protrusions
(11), the side of the hole slot (9) is provided with multiple fixture blocks (91) to match with raised (11), for blocking the sample
This loader mechanism prevents its rotation.
Further, the depth of the hole slot (9) is the sample loader mechanism height of 1/2-2/3, it is ensured that sample loads
Biological sample within the organization is immersed in liquid nitrogen liquid level or less always.
Further, safety cock (71) and/or the freezing storing box for blocking hand basket are provided on the freezing storing box (7)
(7) identification piece person easy to operation is provided on can be quickly found out biological sample.
Further, the sample loader mechanism includes cryopreservation tube (1), freezes load bar (2) and pipe lid (3), described to freeze load
One end of bar (2) and the bottom of the pipe lid (3) are fitting to connection, and described freeze carries the other end of bar (2) as the thin of sample loading
Piece tip (21), the cryopreservation tube (1) are spirally connected with the pipe lid (3) so that the load bar (2) that freezes is fixed on the cryopreservation tube
(1) in, entrance or sky of the through-hole (5) being provided through on cryopreservation tube (1) side or the pipe lid (3) for liquid nitrogen
The discharge of gas.
Further, further include the load bar protective case (41,42) that biological sample can be made to be in enclosure space, the load bar is protected
Sheath (41,42) is one end open, the closed hollow pipe sleeve of the other end；The load bar (2) that freezes is located at the load bar protective case
In (41,42), the load bar protective case (41,42) is located in the cryopreservation tube (1).
Further, the bar protective case that carries is the small funnel type protective case (41) of the big blind end in open end, the funnel type
The open end of protective case (41) is connect with the open top of the cryopreservation tube (1) so that funnel type protective case (41) setting
In the cryopreservation tube (1), side of through-hole (5) setting in the cryopreservation tube (1)；
Alternatively, the bar protective case that carries is the tubular protective sheath (42) for waiting gauge structures, tubular protective sheath (42) are opened
Mouthful end with it is described freeze carry bar (2) top connect so that it is described to freeze load bar (2) setting interior in the tubular protective sheath (42),
The through-hole (5) is arranged on the pipe lid (3).
Further, it is described freeze carry bar (2) its length should be close to the cryopreservation tube (1) pipe shaft height, while not touching again
The tube bottom of the cryopreservation tube (1)；Or the thickness of lamella tip (21) is 0.1-0.3mm, uses poly- the third of highly transparent
Alkene or butadiene-styrene material, and contribute to the operation under mirror and observation under the microscope to be transparent.
Further, cryopreservation tube (1) bottom is designed with the impregnable Quick Response Code under liquid nitrogen environment.
Further, further include holding rod (6), the bottom of the holding rod (6) is provided with multiple apertures (61), described hand-held
Pressing button (62) is provided at the top of bar (6), the pressing button (62) makes institute by the effect of spring and position limiting structure
The shrapnel for stating holding rod (6) bottom is stretched out or is retracted by the aperture (61)；It is described when pressing pressing button (62)
Shrapnel pop-up from the aperture (61), when pressing pressing button (62) again, the shrapnel is retracted into the aperture
(61) in；A three-dimensional cavity (31) is provided at the top of the pipe lid (3), the lower part of the holding rod (6), which can match, is inserted into institute
Three-dimensional cavity (31) is stated, card slot (32) is provided in the solid cavity (31), when the shrapnel is popped up from the aperture (61)
It can be with the fixed latching of the card slot (32).
The biological sample glass frozen cryopreservation device has the advantages that：
(1) biological sample glass frozen cryopreservation device of the present invention, which solves existing cryopreservation device, can only store one layer of sample,
A large amount of the problem of occupying liquid nitrogen container parking space, holding structure of the invention can be superimposed the amount of storage placed and increase sample.
(2) the sample loader mechanism that the depth of the hole slot of biological sample glass frozen cryopreservation device of the present invention is 1/2-2/3 is high
Degree, it is ensured that sample is immersed in liquid nitrogen liquid level hereinafter, when taking out a certain pipe sample always in cryopreservation tube, and liquid is transferred to from liquid nitrogen container
During nitrogen slot, the sample in other cryopreservation tubes also infiltrates in liquid nitrogen always, to reduce sample exposure, ensures its clinic
It uses.
(3) biological sample glass frozen cryopreservation device of the present invention increases identification piece on holding structure and sample loader mechanism
Or Quick Response Code identification piece, in the presence of liquid nitrogen vapor also can rapid localizing sample, reduce the artificial time for finding sample, and two
Dimension code identification piece will not be influenced by liquid nitrogen environment, and after capable of preventing bar code paper from falling off in liquid nitrogen, can not trace to the source its embryo
Information plays the role of dual confirmation to embryo's information.
(4) biological sample glass frozen cryopreservation device of the present invention, which is provided with, carries bar protective case, and sample is made to be in an opposite envelope
The space closed, while not influencing heat transfer efficiency, reduce in liquid nitrogen storage system by the potential cross contamination of liquid nitrogen can
Energy.
(5) biological sample glass frozen cryopreservation device of the present invention is provided with safety cock on freezing storing box, after loading sample, is put into
The freezing storing box of liquid nitrogen hand basket can block liquid nitrogen container hand basket, prevent freezing storing box from sliding.
(6) present invention in cryopreservation tube side or pipe due to covering the through-hole being provided through so that sample is put into liquid nitrogen
When, liquid nitrogen can enter cryopreservation tube quickly through through-hole and be freezed to sample, and not influence air discharge；Sample takes out liquid
When nitrogen, the heated volatilization of liquid nitrogen can quickly be discharged from through-hole, prevent from freezing overpressure increased dramatically and burst.
(7) the pipe lid connection of holding rod and cryopreservation tube of the present invention and separate mode are simple, and hand-held load can be used when fixing
Bar operated under microscope mirror, and holding rod when rotating can be used for that pipe lid is unscrewed or tightened with cryopreservation tube.
Description of the drawings
Fig. 1：The schematic diagram of the funnel type protective case sample loader mechanism of biological sample glass frozen cryopreservation device of the present invention；
Fig. 2：The schematic diagram of the tubular protective sheath sample loader mechanism of biological sample glass frozen cryopreservation device of the present invention；
Fig. 3：The pipe lid stereogram of biological sample glass frozen cryopreservation device of the present invention；
Fig. 4：The pipe lid vertical view of biological sample glass frozen cryopreservation device of the present invention；
Fig. 5：The holding rod schematic diagram of biological sample glass frozen cryopreservation device of the present invention；
Fig. 6：The holding structure schematic diagram of biological sample glass frozen cryopreservation device of the present invention；
Fig. 7：The hole slot vertical view of biological sample glass frozen cryopreservation device of the present invention.
Reference sign：
1- cryopreservation tubes；11- protrusions；2- freezes load bar；21- lamella tips；3- pipe lids；31- solid cavitys；32- card slots；
41- funnel type protective cases；42- tubular protective sheaths；5- through-holes；6- holding rods；61- apertures；62- presses button；7- freezing storing box；71
One safety cock；8- box covers；81- identification pieces；9- hole slots；91- fixture blocks.
Specific implementation mode
With reference to Fig. 1 to Fig. 7, the present invention will be further described：
Biological sample glass frozen cryopreservation device of the present invention, including sample loader mechanism and holding structure, the sample load
Mechanism is for loading biological sample, and the holding structure is for storing the more pieces of sample loader mechanisms, multiple storage machines
Structure overlays in the liquid nitrogen container to increase the amount of storage of biological sample.
Specifically, as depicted in figs. 1 and 2, sample loader mechanism includes cryopreservation tube 1, freezes load bar 2 and pipe lid 3；It freezes
The one end for carrying bar 2 and the bottom of pipe lid 3 are fitting to connection, and are frozen the lamella tip 21 for carrying the other end of bar 2 as sample loading, are frozen
Pipe 1 is spirally connected with pipe lid 3 so that freezes load bar 2 and is fixed in cryopreservation tube 1；The length for freezing load bar 2 should be close to 1 pipe shaft of cryopreservation tube
Highly, the tube bottom of cryopreservation tube 1 while is not touched again, the thickness of lamella tip 21 is 0.1-0.3mm, uses the poly- of highly transparent
Propylene or butadiene-styrene material, and contribute to the operation under mirror and observation under the microscope to be transparent；Cryopreservation tube 1
The through-hole 5 being provided through on side or pipe lid 3 is for the entrance of liquid nitrogen or the discharge of air.
Cryopreservation tube 1 is molded using nontoxic, tasteless, freeze proof, radiation-resistant high-transparency polypropylene material injection；Cryopreservation tube 1
The bar code paper of biological specimen information is posted in side, the bottom of cryopreservation tube 1 be printed on it is associated with biological specimen information on bar code paper
Impregnable Quick Response Code under liquid nitrogen environment；There are four protrusions 11 for the bottom sides setting of cryopreservation tube 1.
Sample loader mechanism is additionally provided with the load bar protective case that biological sample can be made to be in enclosure space, carries bar protective case
For one end open, the closed hollow plastic tube set of the other end, internal diameter is more slightly larger than the diameter of lamella tip 21, about 3mm, freezes load
Bar 2 is located in the load bar protective case, carries bar protective case and is located in cryopreservation tube；
As shown in Figure 1, it is the small funnel type protective case 41 of the big blind end in open end, funnel type protective case 41 to carry bar protective case
Open end connect with the open top of cryopreservation tube 1 so that funnel type protective case 41 is set in cryopreservation tube 1, the side of cryopreservation tube 1
There are two through-holes 5 for face setting.
As shown in Fig. 2, it is the tubular protective sheath 42 for waiting gauge structures, the open end of tubular protective sheath 42 and jelly to carry bar protective case
Deposit the top connection for carrying bar 2 so that freeze and carry the setting of bar 2 in tubular protective sheath 42, there are two through-holes 5 for setting on pipe lid 3.
As shown in figure 5, sample loader mechanism further includes holding rod 6, holding rod 6 is a hollow pressing pen for carrying spring
Bar, length 12-15cm, outer diameter 10-12mm；The bottom of holding rod 6 is provided with multiple apertures 61, and the top of holding rod 6 is set
Be equipped with pressing button 62, pressing button 62 by the effect of spring and position limiting structure so that the shrapnel of 6 bottom of holding rod pass through it is small
Hole 61 is stretched out or retracts；When pressing the pressing button 62, shrapnel is popped up from aperture 61, when pressing pressing button 6 again,
Shrapnel is retracted into aperture 61.
As shown in Figure 3 and Figure 4, the top of pipe lid 3 is provided with a regular polygon cavity, and the lower part of holding rod 6 can match slotting
Enter the regular polygon cavity, card slot 32 is provided in the regular polygon cavity, it can when the shrapnel of holding rod 6 is popped up from aperture
It fixes and locks with card slot 32.
As shown in fig. 6, holding structure includes freezing storing box 7 and box cover 8, box cover 8 and freezing storing box 7 are in the form of paper box
Match, multiple hole slots 9 for fixed sample loader mechanism, the size of hole slot 9 and the sample loading machine are provided on freezing storing box 7
Structure matches, and the depth of hole slot 9 is the sample loader mechanism height of 1/2-2/3, it is ensured that the biological sample in sample loader mechanism
Always it is immersed in liquid nitrogen liquid level or less；The safety cock 71 for blocking hand basket is provided on freezing storing box 7；7 two adjacent surfaces of freezing storing box
On be provided with color apparent letter and number combination mark, box cover 8 is provided with identification piece 81 on one side in face of operator's.
As shown in fig. 7, the bottom sides setting of cryopreservation tube 1 is there are four protrusion 11, the side of hole slot 9 be provided with it is multiple with it is convex
11 fixture blocks 91 to match are played, its rotation is prevented for blocking sample loader mechanism.
It can be seen that the regular polygon cavity at 3 top of lower part matching insertion tube lid of holding rod 6, presses pressing button 62,
Shrapnel is popped up from aperture 61, after being lockked with the card slot 32 in 3 top regular polygon cavity of pipe lid, can form holding rod-pipe lid-
The connection for carrying bar is frozen, rotation holding rod 6 drives pipe lid 3 to be screwed with cryopreservation tube 1, meanwhile, 9 bottom sides of hole slot on freezing storing box 7
The protrusions 11 of fixture block 91 and 1 bottom sides of cryopreservation tube cooperate, can fix jelly when holding rod 6 drives 3 rotation of pipe lid
Deposit pipe 1.
The operation principle of biological sample save set of the present invention is as follows：
Freezing：
1, freezing storing box 7 is positioned in liquid nitrogen tank, liquid nitrogen is made to be saturated with whole hole slots 9 in pedestal.
2, cryopreservation tube 1 is put into the hole slot 9 of freezing storing box 7.
3, by pipe lid 3 and after freezing the load fitting connection of bar 2, by vitrified embryo or egg mother cell in microscope
Under be loaded in freeze carry bar 2 lamella tip 21 on.
4, load bar 2 will be frozen to be put into the cryopreservation tube 1 for carrying bar protective case 41 with funnel type, or will freeze and carry 2 sets of bar
After entering in tubulose load bar protective case 42, together it is put into cryopreservation tube 1.
5, by the regular polygon cavity of 6 lower part insertion tube lid 3 of holding rod, pressing button 62 is pressed, shrapnel is from small hole at bottom part
It popping up in 61, is lockked with the card slot 32 in 3 regular polygon cavity of pipe lid, rotation holding rod 6 drives pipe lid 3 to be screwed with cryopreservation tube 1,
Pressing button 62 is pressed again, and shrapnel is retracted into aperture 61, and holding rod 6 is detached with pipe lid 3.
6, box cover 8 is covered, is put into liquid nitrogen container hand basket, safety cock 71 is buckled.
It thaws：
1, by identification piece 81, the freezing storing box 7 of storage counter sample is found in liquid nitrogen container.
2, safety cock 71 is opened, freezing storing box 7 is taken out, is placed in liquid nitrogen tank, removes box cover 8, then pass through 7 side of freezing storing box
Alphanumeric navigates to target sample.
3, it is checked with the Quick Response Code of 1 bottom of the bar code paper of 1 side of cryopreservation tube and cryopreservation tube, after confirmation is errorless, with holding rod 6
Unscrew pipe lid 3.
4, after taking-up sample subsequent defrosting is carried out according to laboratory operation program, then to sample.
Illustrative description is carried out to the present invention above in conjunction with attached drawing, it is clear that realization of the invention is not by aforesaid way
Limitation, as long as use the inventive concept and technical scheme of the present invention progress various improvement, or it is not improved will be of the invention
Design and technical solution directly apply to other occasions, be within the scope of the invention.
Claims (10)
1. a kind of biological sample glass frozen cryopreservation device, including sample loader mechanism and holding structure, the sample loader mechanism
For loading biological sample, it is characterised in that：The holding structure is multiple described for storing the more pieces of sample loader mechanisms
Holding structure overlays in the liquid nitrogen container to increase the amount of storage of biological sample.
2. biological sample glass frozen cryopreservation device according to claim 1, it is characterised in that：The holding structure includes freezing
Box (7) and box cover (8), the box cover (8) is with the freezing storing box (7) with the form matches of paper box, freezing storing box (7) setting
There are multiple hole slots (9) for fixed sample loader mechanism, size and the sample loader mechanism phase of the hole slot (9)
Match, the side of the sample loader mechanism is provided with multiple raised (11), the side of the hole slot (9) be provided with it is multiple with it is described
The fixture block (91) that raised (11) match, its rotation is prevented for blocking the sample loader mechanism.
3. biological sample glass frozen cryopreservation device according to claim 2, it is characterised in that：The depth of the hole slot (9) is
The sample loader mechanism height, it is ensured that the biological sample in sample loader mechanism is immersed in liquid nitrogen liquid level always
Below.
4. biological sample glass frozen cryopreservation device according to claim 3, it is characterised in that：It is arranged on the freezing storing box (7)
Being useful for being provided with identification piece person easy to operation on the safety cock (71) and/or the freezing storing box (7) that block hand basket can be quick
Find biological sample.
5. according to any one of the claim 1-4 biological sample glass frozen cryopreservation devices, it is characterised in that：The sample dress
Mounted mechanism includes cryopreservation tube (1), freezes load bar (2) and pipe lid (3), described to freeze the one end for carrying bar (2) and the pipe lid (3)
Bottom be fitting to connection, it is described freeze carry bar (2) the other end be sample loading lamella tip (21), the cryopreservation tube (1) with
The pipe lid (3) is spirally connected so that described freeze carries bar (2) and is fixed in the cryopreservation tube (1), cryopreservation tube (1) side or
The through-hole (5) being provided through on pipe lid (3) described in person is for the entrance of liquid nitrogen or the discharge of air.
6. biological sample glass frozen cryopreservation device according to claim 5, it is characterised in that：Further include that can make biological sample
Load bar protective case (41,42) in enclosure space, the load bar protective case (41,42) are one end open, and the other end is closed
Hollow pipe sleeve；The load bar (2) that freezes is located in the load bar protective case (41,42), and the load bar protective case (41,42) is located at
In the cryopreservation tube (1).
7. biological sample glass frozen cryopreservation device according to claim 6, it is characterised in that：
The bar protective case that carries is the small funnel type protective case (41) of the big blind end in open end, the funnel type protective case (41)
Open end is connect with the open top of the cryopreservation tube (1) so that the funnel type protective case (41) is set to the cryopreservation tube
(1) in, side of through-hole (5) setting in the cryopreservation tube (1)；
Alternatively, the bar protective case that carries is the tubular protective sheath (42) for waiting gauge structures, the open end of the tubular protective sheath (42)
It carries the top of bar (2) with described freeze and connect so that described freeze that carry bar (2) setting interior in the tubular protective sheath (42), it is described
Through-hole (5) is arranged on the pipe lid (3).
8. according to any one of the claim 5-7 biological sample glass frozen cryopreservation devices, it is characterised in that：It is described to freeze
Carrying bar (2) its length should be close to the cryopreservation tube (1) pipe shaft height, while not touching the tube bottom of the cryopreservation tube (1) again；Or
Person/and, the thickness of lamella tip (21) is 0.1-0.3mm, uses the polypropylene or butadiene-styrene material of highly transparent
Matter, and contribute to the operation under mirror and observation under the microscope to be transparent.
9. according to any one of the claim 5-8 biological sample glass frozen cryopreservation devices, it is characterised in that：It is described to freeze
Pipe (1) bottom is designed with the impregnable Quick Response Code under liquid nitrogen environment.
10. according to the biological sample glass frozen cryopreservation device of claim 6,7,8 or 9, it is characterised in that：It further include holding rod
(6), the bottom of the holding rod (6) is provided with multiple apertures (61), and pressing button is provided at the top of the holding rod (6)
(62), the pressing button (62) makes the shrapnel of the holding rod (6) bottom pass through by the effect of spring and position limiting structure
The aperture (61) stretches out or retracts；When pressing pressing button (62), shrapnel pop-up from the aperture (61),
When pressing pressing button (62) again, the shrapnel is retracted into the aperture (61)；It is provided at the top of the pipe lid (3)
One three-dimensional cavity (31), the lower part of the holding rod (6), which can match, is inserted into the three-dimensional cavity (31), the solid cavity
(31) it is provided with card slot (32) in, it can be with the fixed latching of the card slot (32) when the shrapnel is popped up from the aperture (61).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810722563.XA CN108617641A (en) | 2018-07-03 | 2018-07-03 | A kind of biological sample glass frozen cryopreservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810722563.XA CN108617641A (en) | 2018-07-03 | 2018-07-03 | A kind of biological sample glass frozen cryopreservation device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108617641A true CN108617641A (en) | 2018-10-09 |
Family
ID=63689597
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810722563.XA Pending CN108617641A (en) | 2018-07-03 | 2018-07-03 | A kind of biological sample glass frozen cryopreservation device |
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| Country | Link |
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| CN (1) | CN108617641A (en) |
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| CN114384098A (en) * | 2021-11-30 | 2022-04-22 | 核工业北京地质研究院 | Sample box for single crystal X-ray diffraction experiment sample and sample preservation method |
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Application publication date: 20181009 |