CN117141903B

CN117141903B - Liquid nitrogen preservation box for stem cell transfer

Info

Publication number: CN117141903B
Application number: CN202311432522.4A
Authority: CN
Inventors: 王雲月
Original assignee: Jilin University
Current assignee: Jilin University
Priority date: 2023-11-01
Filing date: 2023-11-01
Publication date: 2024-01-26
Anticipated expiration: 2043-11-01
Also published as: CN117141903A

Abstract

The invention discloses a liquid nitrogen preservation box for stem cell transfer, which relates to the technical field of liquid nitrogen preservation boxes and comprises a preservation barrel and a cover plate, wherein the cover plate is hinged to the upper part of the preservation barrel, the bottom of the preservation barrel is fixedly connected with a sealing sleeve, the inner wall of the sealing sleeve is rotationally connected with a rotary drum, a plurality of wood sticks are placed in the inner cavity of the rotary drum, and a sealing cover is connected on the cover plate in a threaded manner.

Description

Liquid nitrogen preservation box for stem cell transfer

Technical Field

The invention relates to the technical field of liquid nitrogen preservation boxes, in particular to a liquid nitrogen preservation box for stem cell transfer.

Background

The liquid nitrogen preservation box is used for storing and preserving cells, tissues and biological samples so as to keep the activity and functions of the cells, tissues and biological samples. For stem cell transfer, the effect of the liquid nitrogen preservation box is mainly low-temperature preservation, the liquid nitrogen preservation box can provide extremely low temperature, and the temperature is usually-196 ℃, so that the metabolism of cells can be effectively slowed down or stopped, the survival time of the cells is prolonged, and the stem cells can keep the characteristics and functions of the cells for a longer time at low temperature. The liquid nitrogen in the liquid nitrogen storage box can quickly freeze cells or tissues, so that the cell damage possibly caused in the conventional freezing process is avoided, and the cells and the tissues can be stored in the liquid nitrogen storage box for years or even decades without obvious quality loss due to the ultralow temperature of the liquid nitrogen;

however, for sensitive samples such as stem cells and the like, a low-temperature environment is critical, if the content of liquid nitrogen is insufficient, the temperature in a liquid nitrogen preservation box can rise, the samples can lose cryoprotection and damage or lose effectiveness, and the samples can be damaged or lose vitality, so that the liquid nitrogen content can be timely found and supplemented by periodically measuring the liquid nitrogen content to ensure that a constant low-temperature environment is maintained, and the liquid nitrogen detection is usually carried out by opening the preservation box by a worker to extend a wood stick into the preservation box for detection, and the liquid nitrogen content can be judged by observing the frosting position of the surface of the wood stick, however, when the worker uses the wood stick for liquid nitrogen detection, if the wood stick is placed too deeply in a container, the liquid nitrogen can splash out, so that the freezing injury of the parts such as skin or eyes can be caused, the damage such as pain, blisters, tissue necrosis and the like, and even the risks such as ulcers and infection can be caused;

and the number of times of detecting the liquid nitrogen content is more frequent, frequent uncaps, and touching liquid nitrogen preservation case, if collision or damage liquid nitrogen container carelessly probably can lead to liquid nitrogen leakage, and liquid nitrogen leakage can form a large amount of gases when uncapping to form flammable and explosive environment, thereby caused operational environment's security risk.

In view of this, the present invention thus provides a liquid nitrogen containment vessel for stem cell transfer.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides a liquid nitrogen preservation box for stem cell transfer, which solves the problems in the background art.

In order to achieve the above purpose, the invention is realized by the following technical scheme: the utility model provides a liquid nitrogen preservation case for stem cell shifts, includes and keeps bucket, apron, the apron articulates in the upper portion of keeping the bucket, the bottom fixedly connected with sealed sleeve of keeping the bucket, sealed sleeve inner wall rotates and is connected with the rotary drum, the even fixedly connected with in inner chamber bottom of keeping the bucket is no less than three and places the section of thick bamboo, a plurality of stick has been placed to the rotary drum inner chamber, threaded connection has sealed lid on the apron, be provided with the liquid nitrogen content that is used for short-term test to preserve the bucket inside on the bucket to pop out the quick measurement that the stick reduces the liquid nitrogen loss and pop out the subassembly, be provided with the self-limiting stable subassembly that is used for stabilizing stem cell storage container on the bucket;

the quick test pops out the subassembly including fixed connection in the spring telescopic link of preserving the bucket outer wall, spring telescopic link's upper end fixedly connected with footboard, rotary drum inner wall fixedly connected with cross baffle, rotary drum inner chamber wall evenly runs through the rotation and is connected with multiunit first pop out wheel and second pop out the wheel, and every group first pop out wheel and the outer wall that the second pops out the wheel pass through belt transmission and be connected, the first one end fixedly connected with magnetism runner that pops out the wheel and run through rotary drum outer wall, the outer wall cover of first pop out the wheel is equipped with the torsional spring, the inner wall fixedly connected with magnetism board of preserving the bucket, be provided with the baffle between magnetism board and the magnetism runner, baffle lower part fixedly connected with linking rod, the vertical sliding connection in spout department of preserving the bucket outer wall has sealed longboard, the one end and the sealed longboard fixed connection that the baffle was kept away from to linking rod, the lower extreme and the footboard fixed connection of sealed longboard.

Preferably, the sealing long plate is connected with a sliding shaft in a penetrating and sliding manner, and the outer wall of the rotary drum is fixedly connected with a wavy groove ring.

Preferably, the sliding shaft is positioned in the wavy groove ring, one end of the sliding shaft penetrating through the outer part of the sealing long plate is fixedly connected with a first magnetic block, the outer part of the storage barrel is fixedly connected with a second magnetic block, the first magnetic block and the second magnetic block are opposite magnetic poles, and a limiting spring is sleeved on the outer wall of the sliding shaft.

Preferably, one end of the limiting spring is fixedly connected with the inner wall of the sealing long plate, the other end of the limiting spring is fixedly connected with the outer wall of the sliding shaft, the inner wall of the sealing sleeve is fixedly connected with a leak hole bottom plate, and a notch is formed in the outer surface of the sealing sleeve.

Preferably, the self-limiting stabilizing assembly comprises a connecting rod rotatably connected to the cover plate, a sliding column is rotatably connected to the lower end of the connecting rod, and a sliding sleeve is fixedly connected to the inner wall of the storage barrel.

Preferably, the sliding column penetrates through the sliding sleeve and is connected in the sliding sleeve in a sliding mode, and the lower end of the sliding column is fixedly connected with a bidirectional chute plate.

Preferably, the inner wall fixedly connected with draw runner plate of saving the bucket, symmetrical sliding connection has the slip post in the draw runner plate, slip post runs through sliding connection in two-way inclined groove plate.

Preferably, the sliding column penetrates through one end of the bidirectional chute plate and is fixedly connected with a clamping pad, and the lower part of the bidirectional chute plate is fixedly connected with an L-shaped column.

Preferably, the outer wall of the placing cylinder is provided with a through groove, the L-shaped column penetrates through the groove in a sliding connection mode, and the outer wall of the L-shaped column is uniformly and fixedly connected with a lifting plate which is in sliding connection with the placing cylinder.

The liquid nitrogen preservation box for stem cell transfer provided by the invention has the following beneficial effects:

the two sliding columns are pressed again and the clamping pads are moved close again, so that the clamping pads clamp and position the outer wall of the stem cell storage container on the placing cylinder, the effect of limiting and fixing can be achieved, the condition that the stem cell storage container is tilted and broken and damaged when being transferred and transported in the liquid nitrogen storage barrel in the follow-up process is avoided, and the stability and the integrity of the stem cell storage container during storage are ensured;

when the bidirectional chute plate moves upwards, the L-shaped column and the lifting plate are pulled to move upwards synchronously, so that the stem cell storage container in the placing cylinder can be jacked upwards, a user can observe the label on the stem cell storage container to quickly find out the corresponding stem cell, the user can take the stem cell storage container, the hand of the user is prevented from extending into the liquid nitrogen storage barrel to take the stem cell storage container to cause frostbite, and the purpose of quickly taking the stem cell storage container is achieved;

the liquid nitrogen enters the corresponding wood bar rotary drum from the leak hole at the bottom of the leak hole bottom plate, so that frosting occurs when the surface of the wood bar contacts the liquid nitrogen, the frosting length is the height of the liquid nitrogen liquid level, the aim of measuring the liquid nitrogen content of the wood bar is fulfilled at the moment, then the pedal can be loosened, the spring telescopic rod is reset and rebounded, the first magnetic block at one end of the sliding shaft is not adsorbed with the second magnetic block along with the fact that the sliding shaft is not positioned in the wavy slot ring any more, the slot wall position of the wavy slot ring changes after the sliding shaft rotates, the sliding shaft can not extrude the wavy slot ring and the rotary drum to rotate when the sliding shaft is reset upwards along with the sealing long plate, the aim of replacing the wood bar once after the pedal is stepped on each time can be fulfilled, the aim of replacing the wood bar for detection once is fulfilled, the cover plate is not needed to be manually opened for detection when the wood bar is put into the wood bar, the operation steps required for detection are reduced, and the situation that workers put the wood bar into the liquid nitrogen storage box too deeply to cause freezing damage to the positions such as skin or eyes due to liquid nitrogen splashing is avoided;

when the torsion spring is reset and rebounded, the magnetic rotating wheel, the first popup wheel and the second popup wheel are driven to rotate anticlockwise synchronously, the corresponding outer surface of the wood rod can be rubbed again, the wood rod is enabled to be upwards brought out from the hole position corresponding to the sealing cover, at the moment, a user can take the popup wood rod to observe frosting height, the height of liquid nitrogen in the storage barrel can be timely known, compared with the traditional operation that the liquid nitrogen barrel cover needs to be opened and the wood rod stretches into the operation that the liquid nitrogen content is detected, the situation that the cover plate is opened to cause liquid nitrogen loss can be effectively reduced by the bringing-out mode, further waste of liquid nitrogen is avoided, the liquid nitrogen content in the storage barrel can be ensured to be fully detected by regular detection, and the activity of stem cell sample storage is ensured.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a schematic view of another view angle structure of the present invention;

FIG. 3 is a schematic view of a partial cut-away structure of a holding tank according to the present invention;

FIG. 4 is an enlarged schematic view of the area A in FIG. 3 according to the present invention;

FIG. 5 is a schematic view of the mounting structure of the seal sleeve and the rotary drum of the present invention;

FIG. 6 is an enlarged view of area B of FIG. 5 according to the present invention;

FIG. 7 is an enlarged view of area C of FIG. 5 according to the present invention;

FIG. 8 is a schematic view of the mounting structure of the bi-directional chute plate and L-shaped column of the present invention;

FIG. 9 is a schematic view of the mounting structure of the drum and cross partition of the present invention;

fig. 10 is an enlarged view of the area D of fig. 9 according to the present invention.

In the figure: 1. a storage barrel; 2. a cover plate; 3. a rotating drum; 4. placing a cylinder; 5. a wood stick; 6. sealing cover; 71. a spring telescoping rod; 72. a pedal; 73. a cross partition; 74. a first ejector wheel; 75. a second ejector wheel; 76. a belt; 77. a magnetic wheel; 78. a torsion spring; 79. a magnetic plate; 710. a baffle; 711. a connecting rod; 712. sealing the long plate; 713. a slide shaft; 714. a wave-shaped groove ring; 715. a first magnetic block; 716. a second magnetic block; 717. a leak hole bottom plate; 718. a limit spring;

81. a connecting rod; 82. a spool; 83. a sliding sleeve; 84. a bidirectional chute plate; 85. a chute plate; 86. a sliding column; 87. a clamping pad; 88. an L-shaped column; 89. a lifting plate; 9. and (3) sealing the sleeve.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

Examples

Referring to fig. 1 to 2, a liquid nitrogen preservation box for stem cell transfer comprises a preservation barrel 1 and a cover plate 2, wherein the cover plate 2 is hinged to the upper part of the preservation barrel 1, the bottom of the preservation barrel 1 is fixedly connected with a sealing sleeve 9, the inner wall of the sealing sleeve 9 is rotationally connected with a rotary drum 3, the bottom of an inner cavity of the preservation barrel 1 is uniformly and fixedly connected with at least three placement barrels 4, a plurality of wood sticks 5 are placed in the inner cavity of the rotary drum 3, a sealing cover 6 is connected to the cover plate 2 in a threaded manner, a rapid test ejection assembly for rapidly detecting the liquid nitrogen content in the preservation barrel 1 and ejecting the wood sticks 5 to reduce liquid nitrogen loss is arranged on the preservation barrel 1, and a self-limiting stabilizing assembly for stabilizing a stem cell storage container is arranged on the preservation barrel 1;

referring to fig. 1 to 7 and 9, the fast test ejection assembly includes a spring expansion rod 71 fixedly connected to an outer wall of a storage barrel 1, a pedal 72 is fixedly connected to an upper end of the spring expansion rod 71, a cross partition 73 is fixedly connected to an inner wall of the drum 3, a plurality of groups of first ejection wheels 74 and second ejection wheels 75 are uniformly and rotatably connected to an inner cavity wall of the drum 3 in a penetrating manner, the outer wall of each group of first ejection wheels 74 and the outer wall of the second ejection wheels 75 are in transmission connection through a belt 76, one end of each first ejection wheel 74 penetrating through the outer wall of the drum 3 is fixedly connected with a magnetic rotating wheel 77, a torsion spring 78 is sleeved on the outer wall of each first ejection wheel 74, a magnetic plate 79 is fixedly connected to the inner wall of the storage barrel 1, a baffle 710 is arranged between the magnetic plate 79 and the magnetic rotating wheel 77, a connecting rod 711 is fixedly connected to a lower portion of the baffle 710, a sliding groove of the outer wall of the storage barrel 1 is vertically and slidably connected with a sealing long plate 712, one end of the connecting rod 711 is far away from the sealing long plate 712, the lower end of the sealing long plate 712 is fixedly connected with the pedal 72, and the length of the sealing long plate 712 is greater than the length of the sliding groove 712 of the outer surface of the storage barrel 1, and the sealing long plate can achieve the same magnetic pole effect as the magnetic pole 79 when the magnetic pole is used for sliding the magnetic pole and magnetic pole plate 79 is not lost.

Referring to fig. 1 to 6, a sliding shaft 713 is connected to the seal long plate 712 in a penetrating and sliding manner, a wave-shaped groove ring 714 is fixedly connected to the outer wall of the drum 3, the sliding shaft 713 is located in the wave-shaped groove ring 714, one end of the sliding shaft 713 penetrating the outer portion of the seal long plate 712 is fixedly connected with a first magnetic block 715, a second magnetic block 716 is fixedly connected to the outer portion of the storage barrel 1, the first magnetic block 715 and the second magnetic block 716 are opposite in magnetic pole, a limit spring 718 is sleeved on the outer wall of the sliding shaft 713, one end of the limit spring 718 is fixedly connected with the inner wall of the seal long plate 712, the other end of the limit spring 718 is fixedly connected with the outer wall of the sliding shaft 713, a leak hole bottom plate 717 is fixedly connected to the inner wall of the seal sleeve 9, a notch is formed in the outer surface of the seal sleeve 9, and a leak hole rod is arranged at the leak hole of the leak hole bottom plate 717 and used for blocking the wood rod 5 above the leak hole bottom plate 717 from falling.

Referring to fig. 1 and 8 to 10, the self-limiting stabilizing assembly includes a connecting rod 81 rotatably connected to the cover plate 2, a sliding column 82 rotatably connected to the lower end of the connecting rod 81, a sliding sleeve 83 fixedly connected to the inner wall of the storage barrel 1, and a length of the connecting rod 81 limiting the cover plate 2 to be opened by about 100 degrees, so as to avoid the situation that the cover plate 2 is completely opened and is unfavorable for a subsequent user to rapidly close the cover plate 2.

The sliding columns 82 penetrate through the sliding sleeves 83 and are connected in a sliding manner, the lower ends of the sliding columns 82 are fixedly connected with two-way chute plates 84, and the chute inside the two-way chute plates 84 is arranged in an inclined shape with a wide upper part and a narrow lower part and is used for extruding the two sliding columns 86 to be far away from or close to each other.

Referring to fig. 8 to 10, the inner wall of the storage tub 1 is fixedly connected with a chute plate 85, sliding columns 86 are symmetrically and slidably connected in the chute plate 85, the sliding columns 86 penetrate through and are slidably connected in the bidirectional chute plate 84, one end of the sliding columns 86 penetrating through the bidirectional chute plate 84 is fixedly connected with a clamping pad 87, and the lower portion of the bidirectional chute plate 84 is fixedly connected with an L-shaped column 88. The inner wall of the clamping pad 87 is provided with a rubber pad, so that the elastic clamping effect can be achieved, and the damage of the clamping force to the stem cell storage container is reduced.

The outer wall of the placing barrel 4 is provided with a through groove, the L-shaped column 88 penetrates through the groove and is connected with the groove in a sliding manner, the outer wall of the L-shaped column 88 is uniformly and fixedly connected with the lifting plate 89, the lifting plate 89 is connected with the placing barrel 4 in a sliding manner, and the liquid nitrogen inside the preserving barrel 1 can smoothly enter the placing barrel 4 to effectively refrigerate a stem cell storage container which is placed subsequently through the through groove formed in the outer wall of the placing barrel 4.

The following are all working processes and working principles of the above embodiments:

initial state: the spring telescopic rod 71 is not compressed, the baffle 710 is positioned between the magnetic rotating wheel 77 and the magnetic plate 79, when the storage barrel 1 is used for the first time, the wood rod 5 for measuring the liquid nitrogen content in the storage barrel 1 is firstly placed in the rotary drum 3, the wood rod 5 is uniformly blocked in the cross partition plate 73, then the sealing cover 6 is screwed in a rotating mode, and the cover plate 2 and the storage barrel 1 are closed;

when the device works, firstly, the cover plate 2 is held upwards, at the moment, the originally closed cover plate 2 is rotated upwards to be opened, the connecting rod 81 is pulled to rotate when the cover plate 2 is rotated upwards to further pull the sliding column 82 to slide upwards in the sliding sleeve 83, the sliding column 82 pulls the two sliding columns 84 to synchronously move upwards, the chute on the two sliding columns 84 extrudes the two sliding columns 86 to transversely move away along the sliding chute plate 85, the two fixedly connected clamping pads 87 are pulled to synchronously move away, at the moment, the stem cell storage container is placed in the adaptive placing cylinder 4, specifically, the device can refer to the state of fig. 8 and 9 after the movement, then the cover plate 2 can be closed downwards again, the connecting rod 81 is rotated downwards again, the sliding column 82 is pushed to synchronously move downwards by pressing the two sliding columns 86 and the clamping pads 87 to move close again, the outer walls of the stem cell storage container on the placing cylinder 4 are clamped and positioned by the clamping pads 87, the effect of limiting and fixing can be achieved, the condition that the stem cell storage container is broken in the storage cylinder 1 is stored in a liquid nitrogen transferring and transporting the stem cell storage container is prevented from being damaged, and the storage stability of the stem cell storage container is guaranteed from being broken when the stem cell storage container is in a storage state;

further, when the stem cell storage container needs to be taken out for use, only the cover plate 2 needs to be re-opened, at this time, the connecting rod 81 on the cover plate 2 can pull the sliding column 82 and the bidirectional inclined groove plate 84 upwards, because the bottom of the bidirectional inclined groove plate 84 is fixedly connected with the L-shaped column 88, and the L-shaped column 88 penetrates through the sliding connection to be arranged in the storage barrel 4, and is uniformly and fixedly connected with the lifting plate 89, and the lifting plate 89 is positioned on the inner wall of the storage barrel 4, the bidirectional inclined groove plate 84 can pull the L-shaped column 88 and the lifting plate 89 to synchronously upwards move when moving upwards, so that the stem cell storage container in the storage barrel 4 can be lifted upwards, a user can conveniently observe the label on the stem cell storage container to quickly find out corresponding stem cells, the user can conveniently take the stem cell storage container, the hand of the user can be prevented from extending into the liquid nitrogen storage barrel 1 to take the stem cell storage container, and frostbite can be avoided, and the purpose of quickly taking the stem cell storage container can be achieved;

furthermore, because of the importance of the stem cell storage container stored in the liquid nitrogen storage barrel 1, the liquid nitrogen content in the storage barrel 1 needs to be checked periodically, at this time, only the pedal 72 needs to be depressed downwards to compress the spring telescoping rod 71 downwards, the pedal 72 will drive the sealing long plate 712 fixedly connected with the pedal 72 and the sliding shaft 713 to move downwards, so that the sliding shaft 713 extrudes the wavy groove in the wavy groove ring 714, the wavy groove ring 714 drives the rotary drum 3 to rotate in the sealing sleeve 9, when the sliding shaft 713 moves downwards to be at the same height as the magnetic block two 716, the magnetic block one 715 adsorbs with the magnetic block two 716, and then the sliding shaft 713 adsorbs and slides on the sealing long plate 712 to be attached to the magnetic block two 716, so that the sliding shaft 713 is separated from the wavy groove ring 714 and compresses the limiting spring 718, when the wavy groove ring 714 rotates, the rotary drum 3 drives the wooden stick 5 in the rotary drum to rotate simultaneously, the old wooden stick 5 is replaced, the new wooden stick 5 is positioned above the perforated bottom plate 717, liquid nitrogen enters the corresponding wooden stick 5 rotary drum 3 from the perforated bottom plate 717, frosting is caused by contact of the surface of the wooden stick 5 with the liquid nitrogen, the frosting length is the height of the liquid nitrogen liquid level, the purpose of measuring the liquid nitrogen content of the wooden stick 5 is achieved, the pedal 72 can be loosened, at the moment, the spring telescopic rod 71 is reset and rebounded, the first magnetic block 715 at one end of the sliding shaft 713 is not adsorbed with the second magnetic block 716 any more, and the groove wall position of the sliding shaft 713 is changed after the sliding shaft 713 rotates in the wavy groove ring 714, so that the sliding shaft 713 does not extrude the wavy groove ring 714 and the rotary drum 3 to rotate when the sliding shaft 713 is reset upwards along with the sealing long plate 712, the wooden stick 5 can be prevented from being replaced again, the purpose that the wooden stick 5 can be replaced for detection once by stepping the pedal 72 once every time is achieved, the wooden stick 5 is not required to be put into the cover plate 2 for detection by manual operation, the operation steps required by detection are reduced, and the situation that the wooden stick 5 is put into a liquid nitrogen storage box too deeply by workers, and the skin or eyes and other parts are damaged by freezing caused by liquid nitrogen splashing is avoided;

it should be noted that, because one end of the connecting rod 711 is fixedly connected with the sealing long plate 712, when the sealing long plate 712 moves downward along with the pedal 72, the baffle 710 at the upper end is pulled by the connecting rod 711 to synchronously descend, so that the baffle 710 is no longer located between the magnetic rotating wheel 77 and the magnetic plate 79, because the magnetic rotating wheel 77 and the magnetic plate 79 are the same magnetic poles, the magnetic rotating wheel 77 rotates clockwise and drives the torsion spring 78 to shrink, and the magnetic rotating wheel 77 rotates to synchronously rotate the first ejecting wheel 74, because the first ejecting wheel 74 is in transmission connection with the second ejecting wheel 75 through the belt 76, the first ejecting wheel 74 rotates clockwise with the second ejecting wheel 75 and rubs the outer wall of the wood bar 5, after the pedal 72 stays to give the wood bar 5 a certain detection time, the sealing cover 6 on the cover plate 2 is firstly rotated and removed, the loosening pedal 72 drives the sealing long plate 712 to reset upwards, the connecting rod 711 and the baffle 710 are synchronously driven to reset and block the space between the magnetic rotating wheel 77 and the magnetic plate 79, at the moment, the magnetic rotating wheel 77 is not adsorbed by the magnetic plate 79 any more, so that the torsion spring 78 can drive the magnetic rotating wheel 77, the first ejecting wheel 74 and the second ejecting wheel 75 to rotate anticlockwise synchronously, the outer surface of the corresponding wood rod 5 can be rubbed again, the wood rod 5 is brought up from the position of the corresponding hole of the sealing cover 6, at the moment, a user can take the ejected wood rod 5 to observe frosting height, the height of liquid nitrogen in the storage barrel 1 can be known in time, compared with the traditional operation that the liquid nitrogen barrel cover needs to be opened to extend into the wood rod 5 to detect the content of liquid nitrogen, the bringing-out mode can effectively reduce the situation that the liquid nitrogen is lost when the cover plate 2 is opened, further avoiding the waste of liquid nitrogen, and the periodic detection can ensure that the liquid nitrogen content in the preservation barrel 1 is sufficient, and the preservation activity of the stem cell sample is ensured.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A liquid nitrogen preservation case for stem cell shifts, includes and preserves bucket (1), apron (2), its characterized in that: the cover plate (2) is hinged to the upper portion of the preservation barrel (1), a sealing sleeve (9) is fixedly connected to the bottom of the preservation barrel (1), a rotary drum (3) is rotatably connected to the inner wall of the sealing sleeve (9), at least three placement barrels (4) are uniformly and fixedly connected to the bottom of an inner cavity of the preservation barrel (1), a plurality of wood sticks (5) are placed in the inner cavity of the rotary drum (3), a sealing cover (6) is connected to the cover plate (2) in a threaded mode, a rapid test ejection assembly for rapidly detecting the liquid nitrogen content in the preservation barrel (1) and ejecting the wood sticks (5) to reduce liquid nitrogen loss is arranged on the preservation barrel (1), and a self-limiting stabilizing assembly for stabilizing a dry cell storage container is arranged on the preservation barrel (1);

the quick test popup assembly comprises a spring telescopic rod (71) fixedly connected to the outer wall of a preservation barrel (1), a pedal (72) is fixedly connected to the upper end of the spring telescopic rod (71), a cross partition plate (73) is fixedly connected to the inner wall of the rotary barrel (3), a plurality of groups of first popup wheels (74) and second popup wheels (75) are uniformly and rotatably connected to the inner cavity wall of the rotary barrel (3) in a penetrating mode, the outer wall of each group of first popup wheels (74) and the outer wall of each second popup wheel (75) are in transmission connection through a belt (76), one end of each first popup wheel (74) penetrates through one end of the outer wall of the rotary barrel (3) and is fixedly connected with a magnetic rotating wheel (77), a torsion spring (78) is sleeved on the outer wall of each first popup wheel (74), a magnetic plate (79) is fixedly connected with one end of each magnetic rotating wheel (77), a baffle plate (710) is arranged between each magnetic plate (79) and each magnetic rotating wheel (77), a connecting rod (711) is fixedly connected to the lower portion of each baffle plate (710), a sealing long plate (712) is vertically and slidably connected to a chute of the outer wall of the preservation barrel (1), and one end of each sealing plate (712) is far away from the fixed connection end of the long plate (712).

A sliding shaft (713) is connected to the sealing long plate (712) in a penetrating and sliding manner, and a wavy groove ring (714) is fixedly connected to the outer wall of the rotary drum (3);

the sliding shaft (713) is positioned in the wavy groove ring (714), one end of the sliding shaft (713) penetrating through the outside of the sealing long plate (712) is fixedly connected with a first magnetic block (715), the outside of the storage barrel (1) is fixedly connected with a second magnetic block (716), the first magnetic block (715) and the second magnetic block (716) are opposite in magnetic pole, and a limiting spring (718) is sleeved on the outer wall of the sliding shaft (713);

one end of the limiting spring (718) is fixedly connected with the inner wall of the sealing long plate (712), the other end of the limiting spring (718) is fixedly connected with the outer wall of the sliding shaft (713), the inner wall of the sealing sleeve (9) is fixedly connected with a leak hole bottom plate (717), and a notch is formed in the outer surface of the sealing sleeve (9);

the self-limiting stabilizing assembly comprises a connecting rod (81) rotatably connected to the cover plate (2), a sliding column (82) is rotatably connected to the lower end of the connecting rod (81), and a sliding sleeve (83) is fixedly connected to the inner wall of the storage barrel (1);

the sliding column (82) is connected in the sliding sleeve (83) in a penetrating and sliding manner, and the lower end of the sliding column (82) is connected with a bidirectional chute plate (84);

the inner wall of the storage barrel (1) is fixedly connected with a chute plate (85), sliding columns (86) are symmetrically and slidingly connected in the chute plate (85), and the sliding columns (86) penetrate through and are slidingly connected in the bidirectional chute plate (84);

the sliding column (86) penetrates through one end of the bidirectional inclined groove plate (84) and is fixedly connected with a clamping pad (87), and the lower part of the bidirectional inclined groove plate (84) is fixedly connected with an L-shaped column (88);

the outer wall of the placement barrel (4) is provided with a through groove, the L-shaped column (88) is connected in the groove in a penetrating and sliding mode, the outer wall of the L-shaped column (88) is uniformly and fixedly connected with a lifting plate (89), and the lifting plate (89) is connected in the placement barrel (4) in a sliding mode.