CN208798591U - A kind of biological sample glass frozen cryopreservation device - Google Patents
A kind of biological sample glass frozen cryopreservation device Download PDFInfo
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- CN208798591U CN208798591U CN201821048526.7U CN201821048526U CN208798591U CN 208798591 U CN208798591 U CN 208798591U CN 201821048526 U CN201821048526 U CN 201821048526U CN 208798591 U CN208798591 U CN 208798591U
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- biological sample
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Abstract
The utility model relates to a kind of biological sample glass frozen cryopreservation devices, including sample loader mechanism and holding structure, the sample loader mechanism is for loading biological sample, for the holding structure for storing the more pieces of sample loader mechanisms, multiple holding structures overlay the amount of storage for increasing biological sample in liquid nitrogen container.The utility model biological sample save set solves existing cryopreservation device due to being all slender type sticking plaster, can only store one layer of sample, thus a large amount of the problem of occupying liquid nitrogen container parking space, and it is easy to operate, sample can be positioned rapidly, prevents embryo from obscuring, and reduce pollution risk.
Description
Technical field
The utility model relates to a kind of biological sample save set, especially a kind of biological sample glass frozen cryopreservation device.
Background technique
Be used to save frozen embryo or egg mother cell currently, in supplementary reproduction field, in laboratory predominantly freezes load
Bar and freeze straw.As described in Chinese patent literature CN 106489914A, freezing carries the modeling that bar is non-isodiametric elongated shape
Charge bar, taper end are used to sample loading, tubbiness is handheld terminal close to lamella tip.Straw is the hollow plastic stick of strip.One end
Connection loads the load bar of sample, and the other end is the veil containing cotton thread of filtration, and prevents the steel loop floated in liquid nitrogen.It presses
Such mode, one layer of sample can only be stored since the two is all slender type sticking plaster by carrying bar cooperation freezing straw using freezing,
Occupy larger space in liquid nitrogen container.With the increase of infertile crowd in recent years, receive the patient of embryo transfer also increasingly
It is more, it is annual newly-increased to freeze embryo's quantity and also increase.Many reproductive centers increase more than ten or even tens liquid nitrogen containers newly every year, with full
The growing embryo freezing of foot needs.
In addition, the existing load bar that freezes is in semi-closed state, there are the risks of sample cross contamination.Meanwhile defrosting sample
When product, due to the influence of steam above liquid nitrogen, the frosting of bar rack surface is carried, the label above bracket is covered, can not be mentioned from one
Bucket quickly finds sample, extends the artificial time for finding sample.
Utility model content
The technical issues of the utility model devises a kind of biological sample glass frozen cryopreservation device, solves is existing jelly
Cryopreservation device can only store one layer of sample, thus a large amount of parking spaces for occupying liquid nitrogen container due to being all slender type sticking plaster.
In order to solve above-mentioned technical problem, the utility model uses following scheme:
A kind of biological sample glass frozen cryopreservation device, including sample loader mechanism and holding structure, the sample loading machine
Structure is for loading biological sample, and the holding structure is for storing the more pieces of sample loader mechanisms, multiple holding structures
Overlay the amount of storage for increasing biological sample in the liquid nitrogen container.
Further, the holding structure includes freezing storing box (7) and box cover (8), the box cover (8) and the freezing storing box (7)
With the form matches of paper box, the freezing storing box (7) is provided with multiple hole slots (9) for fixed sample loader mechanism, described
The size of hole slot (9) matches with the sample loader mechanism, and the side of the sample loader mechanism is provided with multiple protrusions
(11), the side of the hole slot (9) is provided with multiple fixture blocks (91) to match with raised (11), for blocking the sample
This loader mechanism prevents its rotation.
Further, the depth of the hole slot (9) is the sample loader mechanism height of 1/2-2/3, it is ensured that sample loads
Biological sample within the organization is immersed in liquid nitrogen liquid level or less always.
Further, safety cock (71) and/or the freezing storing box for blocking hand basket are provided on the freezing storing box (7)
(7) identification piece is provided on can be quickly found out biological sample convenient for operator.
Further, the sample loader mechanism includes cryopreservation tube (1), freezes load bar (2) and pipe lid (3), described to freeze load
One end of bar (2) and the bottom of the pipe lid (3) are fitting to connection, and the other end for freezing load bar (2) is the thin of sample loading
Piece tip (21), the cryopreservation tube (1) are spirally connected with the pipe lid (3), so that load bar (2) that freezes is fixed on the cryopreservation tube
(1) in, the entrance or sky of perforative through-hole (5) for liquid nitrogen are provided on cryopreservation tube (1) side or the pipe lid (3)
The discharge of gas.
It further, further include the load bar protective case (41,42) that biological sample can be made to be in enclosure space, the load bar is protected
Sheath (41,42) is open at one end, the closed hollow pipe sleeve of the other end；Load bar (2) that freezes is located at the load bar protective case
In (41,42), the load bar protective case (41,42) is located in the cryopreservation tube (1).
Further, the bar protective case that carries is the small funnel type protective case (41) in the big closed end in open end, the funnel type
The open end of protective case (41) is connect with the top opening of the cryopreservation tube (1), so that the funnel type protective case (41) is arranged
In the cryopreservation tube (1), side of through-hole (5) setting in the cryopreservation tube (1)；
Alternatively, the bar protective case that carries is the tubular protective sheath (42) for waiting gauge structures, tubular protective sheath (42) are opened
Mouthful end with it is described freeze carry bar (2) top connect so that it is described freeze carry bar (2) be arranged it is interior in the tubular protective sheath (42),
The through-hole (5) is arranged on the pipe lid (3).
Further, it is described freeze carry bar (2) its length should be close to the cryopreservation tube (1) pipe shaft height, while not touching again
The tube bottom of the cryopreservation tube (1)；Or lamella tip (21) with a thickness of 0.1-0.3mm, use poly- the third of highly transparent
Alkene or butadiene-styrene material, and be under the microscope the transparent operation and observation facilitated under mirror.
Further, cryopreservation tube (1) the bottom design has the impregnable two dimensional code under liquid nitrogen environment.
It further, further include holding rod (6), the bottom of the holding rod (6) is provided with multiple apertures (61), described hand-held
It is provided at the top of bar (6) push button (62), the push button (62) is by the effect of spring and position limiting structure so that institute
The elastic slice for stating holding rod (6) bottom is stretched out or is retracted by the aperture (61)；It is described when pressing the push button (62)
Elastic slice pop-up from the aperture (61), when pressing the push button (62) again, the elastic slice is retracted into the aperture
(61) in；A three-dimensional cavity (31) is provided at the top of the pipe lid (3), the lower part of the holding rod (6) can match insertion institute
It states three-dimensional cavity (31), is provided with card slot (32) in the solid cavity (31), when the elastic slice is popped up from the aperture (61)
It can be with the card slot (32) fixed latching.
The biological sample glass frozen cryopreservation device has the advantages that
(1) the utility model biological sample glass frozen cryopreservation device, which solves existing cryopreservation device, can only store one layer of sample
Product, a large amount of the problem of occupying liquid nitrogen container parking space, the holding structure of the utility model can be superimposed placement and increase sample
Amount of storage.
(2) depth of the hole slot of the utility model biological sample glass frozen cryopreservation device is the sample loading machine of 1/2-2/3
Structure height, it is ensured that sample is immersed in liquid nitrogen liquid level hereinafter, when taking out a certain pipe sample always in cryopreservation tube, shifts from liquid nitrogen container
During liquid nitrogen tank, the sample in other cryopreservation tubes also infiltrates in liquid nitrogen always, to reduce sample exposure, guarantees it
Clinical use.
(3) the utility model biological sample glass frozen cryopreservation device increases mark on holding structure and sample loader mechanism
Know part or two dimensional code identification piece, in the presence of liquid nitrogen vapor also can rapid localizing sample, reduce the artificial time for finding sample, and
And two dimensional code identification piece not will receive the influence of liquid nitrogen environment, after capable of preventing bar code paper from falling off in liquid nitrogen, can not trace to the source it
Embryo's information plays the role of dual confirmation to embryo's information.
(4) the utility model biological sample glass frozen cryopreservation device, which is provided with, carries bar protective case, and sample is made to be in a phase
To closed space, while not influencing heat transfer efficiency, reduce in liquid nitrogen storage system through the potential cross contamination of liquid nitrogen
Possibility.
(5) the utility model biological sample glass frozen cryopreservation device is provided with safety cock on freezing storing box, after loading sample,
The freezing storing box for being put into liquid nitrogen hand basket can block liquid nitrogen container hand basket, prevent freezing storing box from sliding.
(6) the utility model is provided with perforative through-hole due to covering in cryopreservation tube side or pipe, so that sample is put into
When liquid nitrogen, liquid nitrogen can enter cryopreservation tube quickly through through-hole and freeze to sample, and not influence air discharge；Sample takes
Out when liquid nitrogen, the heated volatilization of liquid nitrogen can quickly be discharged from through-hole, and preventing from freezing overpressure increased dramatically and burst.
(7) connection of the pipe lid of the utility model holding rod and cryopreservation tube and separate mode are simple, and fixed Shi Keyong hand
It holds to carry and be operated under bar progress microscope mirror, holding rod when rotating can be used for that pipe lid is unscrewed or tightened with cryopreservation tube.
Detailed description of the invention
Fig. 1: the signal of the funnel type protective case sample loader mechanism of the utility model biological sample glass frozen cryopreservation device
Figure；
Fig. 2: the schematic diagram of the tubular protective sheath sample loader mechanism of the utility model biological sample glass frozen cryopreservation device；
Fig. 3: the pipe lid perspective view of the utility model biological sample glass frozen cryopreservation device；
Fig. 4: the pipe lid top view of the utility model biological sample glass frozen cryopreservation device；
Fig. 5: the holding rod schematic diagram of the utility model biological sample glass frozen cryopreservation device；
Fig. 6: the holding structure schematic diagram of the utility model biological sample glass frozen cryopreservation device；
Fig. 7: the hole slot top view of the utility model biological sample glass frozen cryopreservation device.
Description of symbols:
1- cryopreservation tube；11- protrusion；2- freezes load bar；21- lamella tip；3- pipe lid；31- solid cavity；32- card slot；
41- funnel type protective case；42- tubular protective sheath；5- through-hole；6- holding rod；61- aperture；62- push button；7- freezing storing box；
71- safety cock；8- box cover；81- identification piece；9- hole slot；91- fixture block.
Specific embodiment
Below with reference to Fig. 1 to Fig. 7, the utility model is described further:
The utility model biological sample glass frozen cryopreservation device, including sample loader mechanism and holding structure, the sample
For loader mechanism for loading biological sample, the holding structure is multiple described to deposit for storing the more pieces of sample loader mechanisms
Laying mechanism overlays the amount of storage for increasing biological sample in the liquid nitrogen container.
Specifically, as depicted in figs. 1 and 2, sample loader mechanism includes cryopreservation tube 1, freezes load bar 2 and pipe lid 3；It freezes
The bottom of one end and pipe lid 3 for carrying bar 2 is fitting to connection, and is frozen the lamella tip 21 for carrying the other end of bar 2 as sample loading, is frozen
Pipe 1 is spirally connected with pipe lid 3, is fixed in cryopreservation tube 1 so that freezing and carrying bar 2；The length for freezing load bar 2 should be close to 1 pipe shaft of cryopreservation tube
Highly, while again do not touch the tube bottom of cryopreservation tube 1, lamella tip 21 with a thickness of 0.1-0.3mm, use the poly- of highly transparent
Propylene or butadiene-styrene material, and be under the microscope the transparent operation and observation facilitated under mirror；Cryopreservation tube 1
Perforative through-hole 5 is provided on side or pipe lid 3 for the entrance of liquid nitrogen or the discharge of air.
Cryopreservation tube 1 is formed using nontoxic, tasteless, freeze proof, anti-radiation high-transparency polypropylene material injection；Cryopreservation tube 1
The bar code paper of biological specimen information is posted in side, the bottom of cryopreservation tube 1 be printed on it is associated with biological specimen information on bar code paper
Impregnable two dimensional code under liquid nitrogen environment；There are four protrusions 11 for the bottom sides setting of cryopreservation tube 1.
Sample loader mechanism is additionally provided with the load bar protective case that biological sample can be made to be in enclosure space, carries bar protective case
To be open at one end, the closed hollow plastic tube set of the other end, internal diameter is more slightly larger than the diameter of lamella tip 21, about 3mm, freezes load
Bar 2 is located in the load bar protective case, carries bar protective case and is located in cryopreservation tube；
As shown in Figure 1, carrying bar protective case is the small funnel type protective case 41 in the big closed end in open end, funnel type protective case 41
Open end connect with the top opening of cryopreservation tube 1 so that funnel type protective case 41 is set in cryopreservation tube 1, the side of cryopreservation tube 1
There are two through-holes 5 for face setting.
As shown in Fig. 2, carrying bar protective case is the tubular protective sheath 42 for waiting gauge structures, the open end of tubular protective sheath 42 and jelly
The top connection for carrying bar 2 is deposited, is arranged in tubular protective sheath 42 so that freezing and carrying bar 2, there are two through-holes 5 for setting on pipe lid 3.
As shown in figure 5, sample loader mechanism further includes holding rod 6, holding rod 6 is the hollow pressing pen for having spring
Bar, length 12-15cm, outer diameter 10-12mm；The bottom of holding rod 6 is provided with multiple apertures 61, and the top of holding rod 6 is set
Be equipped with push button 62, push button 62 by the effect of spring and position limiting structure so that the elastic slice of 6 bottom of holding rod pass through it is small
Hole 61 is stretched out or is retracted；When pressing the push button 62, elastic slice is popped up from aperture 61, when pressing push button 6 again,
Elastic slice is retracted into aperture 61.
As shown in Figure 3 and Figure 4, a regular polygon cavity is provided at the top of pipe lid 3, the lower part of holding rod 6 can match slotting
Enter the regular polygon cavity, is provided with card slot 32 in the regular polygon cavity, it can when the elastic slice of holding rod 6 is popped up from aperture
With the fixed latching of card slot 32.
As shown in fig. 6, holding structure includes freezing storing box 7 and box cover 8, box cover 8 and freezing storing box 7 are in the form of paper box
Match, multiple hole slots 9 for fixed sample loader mechanism, the size of hole slot 9 and the sample loading machine are provided on freezing storing box 7
Structure matches, and the depth of hole slot 9 is the sample loader mechanism height of 1/2-2/3, it is ensured that the biological sample in sample loader mechanism
Always it is immersed in liquid nitrogen liquid level or less；The safety cock 71 for blocking hand basket is provided on freezing storing box 7；7 two adjacent surfaces of freezing storing box
On be provided with color apparent letter and number combination mark, box cover 8 is provided with identification piece 81 in face of operator's on one side.
As shown in fig. 7, cryopreservation tube 1 bottom sides setting there are four protrusion 11, the side of hole slot 9 be provided with it is multiple with it is convex
11 fixture blocks 91 to match are played, prevent its rotation for blocking sample loader mechanism.
It can be seen that the regular polygon cavity at 3 top of lower part matching insertion tube lid of holding rod 6, presses push button 62,
Elastic slice is popped up from aperture 61, after lockking with the card slot 32 in 3 top regular polygon cavity of pipe lid, can form holding rod-pipe lid-
The connection for carrying bar is frozen, rotation holding rod 6 drives pipe lid 3 to screw with cryopreservation tube 1, meanwhile, 9 bottom sides of hole slot on freezing storing box 7
Fixture block 91 and 1 bottom sides of cryopreservation tube protrusion 11 cooperate, holding rod 6 drive pipe lid 3 rotation when be capable of fixing jelly
Deposit pipe 1.
The working principle of the utility model biological sample save set is as follows:
Freezing:
1, freezing storing box 7 is placed in liquid nitrogen tank, liquid nitrogen is made to be saturated with whole hole slots 9 in pedestal.
2, cryopreservation tube 1 is put into the hole slot 9 of freezing storing box 7.
3, by pipe lid 3 and after freezing the load fitting connection of bar 2, by vitrified embryo or egg mother cell in microscope
Under be loaded in freeze carry bar 2 lamella tip 21 on.
4, load bar 2 will be frozen to be put into the cryopreservation tube 1 for carrying bar protective case 41 with funnel type, or 2 sets of bar of load will be frozen
After entering in tubulose load bar protective case 42, together it is put into cryopreservation tube 1.
5, by the regular polygon cavity of 6 lower part insertion tube lid 3 of holding rod, push button 62 is pressed, elastic slice is from small hole at bottom part
It popping up in 61, is lockked with the card slot 32 in 3 regular polygon cavity of pipe lid, rotation holding rod 6 drives pipe lid 3 to screw with cryopreservation tube 1,
Push button 62 is pressed again, and elastic slice is retracted into aperture 61, and holding rod 6 is separated with pipe lid 3.
6, box cover 8 is covered, is put into liquid nitrogen container hand basket, safety cock 71 is buckled.
It thaws:
1, by identification piece 81, the freezing storing box 7 of storage counter sample is found in liquid nitrogen container.
2, safety cock 71 is opened, freezing storing box 7 is taken out, is placed in liquid nitrogen tank, removes box cover 8, then pass through 7 side of freezing storing box
Alphanumeric navigates to target sample.
3, it is checked with the two dimensional code of 1 bottom of the bar code paper of 1 side of cryopreservation tube and cryopreservation tube, after confirmation is errorless, with holding rod 6
Unscrew pipe lid 3.
4, subsequent defrosting is carried out according to laboratory operation program, then to sample after taking-up sample.
Illustrative description has been carried out to the utility model above in conjunction with attached drawing, it is clear that the realization of the utility model not by
The limitation of aforesaid way, if the method concept of the utility model and the various improvement of technical solution progress are used, or without
It improves and the conception and technical scheme of the utility model is directly applied into other occasions, in the protection scope of the utility model
It is interior.
Claims (10)
1. a kind of biological sample glass frozen cryopreservation device, including sample loader mechanism and holding structure, the sample loader mechanism
For loading biological sample, it is characterised in that: the holding structure is multiple described for storing the more pieces of sample loader mechanisms
Holding structure overlays the amount of storage for increasing biological sample in liquid nitrogen container.
2. biological sample glass frozen cryopreservation device according to claim 1, it is characterised in that: the holding structure includes freezing
Box (7) and box cover (8), the box cover (8) and the freezing storing box (7) are with the form matches of paper box, freezing storing box (7) setting
There are multiple hole slots (9) for fixed sample loader mechanism, size and the sample loader mechanism phase of the hole slot (9)
Match, the side of the sample loader mechanism is provided with multiple raised (11), the side of the hole slot (9) be provided with it is multiple with it is described
The fixture block (91) that raised (11) match, prevents its rotation for blocking the sample loader mechanism.
3. biological sample glass frozen cryopreservation device according to claim 2, it is characterised in that: the depth of the hole slot (9) is
The sample loader mechanism height, it is ensured that the biological sample in sample loader mechanism is immersed in liquid nitrogen liquid level always
Below.
4. biological sample glass frozen cryopreservation device according to claim 3, it is characterised in that: be arranged on the freezing storing box (7)
Have be provided on safety cock (71) and/or the freezing storing box (7) for blocking hand basket identification piece convenient for operator can be quick
Find biological sample.
5. biological sample glass frozen cryopreservation device described in any one of -4 according to claim 1, it is characterised in that: the sample dress
Mounted mechanism includes cryopreservation tube (1), freezes load bar (2) and pipe lid (3), described to freeze the one end for carrying bar (2) and the pipe lid (3)
Bottom be fitting to connection, it is described freeze carry bar (2) the other end be sample loading lamella tip (21), the cryopreservation tube (1) with
The pipe lid (3) is spirally connected so that described freeze carries bar (2) and is fixed in the cryopreservation tube (1), cryopreservation tube (1) side or
Perforative through-hole (5) are provided on pipe lid (3) described in person for the entrance of liquid nitrogen or the discharge of air.
6. biological sample glass frozen cryopreservation device according to claim 5, it is characterised in that: further include that can make biological sample
Load bar protective case (41,42) in enclosure space, the load bar protective case (41,42) be it is open at one end, the other end is closed
Hollow pipe sleeve；Load bar (2) that freezes is located in the load bar protective case (41,42), and the load bar protective case (41,42) is located at
In the cryopreservation tube (1).
7. biological sample glass frozen cryopreservation device according to claim 6, it is characterised in that:
The bar protective case that carries is the small funnel type protective case (41) in the big closed end in open end, the funnel type protective case (41)
Open end is connect with the top opening of the cryopreservation tube (1), so that the funnel type protective case (41) is set to the cryopreservation tube
(1) in, side of through-hole (5) setting in the cryopreservation tube (1)；
Alternatively, the bar protective case that carries is the tubular protective sheath (42) for waiting gauge structures, the open end of the tubular protective sheath (42)
It carries the top of bar (2) with described freeze and connect so that described freeze that carry bar (2) setting interior in the tubular protective sheath (42), it is described
Through-hole (5) is arranged on the pipe lid (3).
8. biological sample glass frozen cryopreservation device according to claim 5, it is characterised in that: described freeze carries bar (2) its length
Degree should be close to the cryopreservation tube (1) pipe shaft height, while not touching the tube bottom of the cryopreservation tube (1) again；Or thin slice point
Hold (21) with a thickness of 0.1-0.3mm, use the polypropylene or butadiene-styrene material of highly transparent, and micro-
It is the transparent operation and observation facilitated under mirror under mirror.
9. biological sample glass frozen cryopreservation device according to claim 6, it is characterised in that: cryopreservation tube (1) bottom is set
In respect of two dimensional code impregnable under liquid nitrogen environment.
10. according to claim 6,7,8 or the 9 biological sample glass frozen cryopreservation devices, it is characterised in that: further include holding rod
(6), the bottom of the holding rod (6) is provided with multiple apertures (61), is provided with push button at the top of the holding rod (6)
(62), the push button (62) is by the effect of spring and position limiting structure so that the elastic slice of the holding rod (6) bottom passes through
The aperture (61) is stretched out or is retracted；When pressing the push button (62), the elastic slice is hit by a bullet from the aperture (61)
Out, when pressing the push button (62) again, the elastic slice is retracted into the aperture (61)；It is set at the top of the pipe lid (3)
It is equipped with a three-dimensional cavity (31), the lower part of the holding rod (6) can match the insertion three-dimensional cavity (31), described three-dimensional empty
It is provided with card slot (32) in chamber (31), the elastic slice can be with the card slot (32) fixing lock when popping up from the aperture (61)
Firmly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821048526.7U CN208798591U (en) | 2018-07-03 | 2018-07-03 | A kind of biological sample glass frozen cryopreservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821048526.7U CN208798591U (en) | 2018-07-03 | 2018-07-03 | A kind of biological sample glass frozen cryopreservation device |
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| Publication Number | Publication Date |
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| CN208798591U true CN208798591U (en) | 2019-04-30 |
Family
ID=66228408
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821048526.7U Active CN208798591U (en) | 2018-07-03 | 2018-07-03 | A kind of biological sample glass frozen cryopreservation device |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110583628A (en) * | 2019-10-11 | 2019-12-20 | 山东山大附属生殖医院有限公司 | Biological cell refrigerating device convenient to search |
| EP4026426A4 (en) * | 2019-09-06 | 2023-10-25 | Suzhou Basecare Medical Device Co., Ltd. | Vitrification freezing carrier |
-
2018
- 2018-07-03 CN CN201821048526.7U patent/CN208798591U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4026426A4 (en) * | 2019-09-06 | 2023-10-25 | Suzhou Basecare Medical Device Co., Ltd. | Vitrification freezing carrier |
| CN110583628A (en) * | 2019-10-11 | 2019-12-20 | 山东山大附属生殖医院有限公司 | Biological cell refrigerating device convenient to search |
| CN110583628B (en) * | 2019-10-11 | 2021-08-24 | 山东山大附属生殖医院有限公司 | Biological cell refrigerating device convenient to search |
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