CN110583628B - Biological cell refrigerating device convenient to search - Google Patents
Biological cell refrigerating device convenient to search Download PDFInfo
- Publication number
- CN110583628B CN110583628B CN201910962030.3A CN201910962030A CN110583628B CN 110583628 B CN110583628 B CN 110583628B CN 201910962030 A CN201910962030 A CN 201910962030A CN 110583628 B CN110583628 B CN 110583628B
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- storage
- storage rack
- sliding
- freezing
- main rod
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0263—Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving, e.g. cool boxes, blood bags or "straws" for cryopreservation
Abstract
The biological cell freezing device convenient for searching is used for facilitating the storage and extraction of biological samples. Including a jar body, be fixed with the backing ring at the jar mouthful inner wall of jar body, still include: the first storage unit comprises vertical rods fixed on the inner wall of the tank body and a first storage rack arranged between every two adjacent vertical rods in a radial sliding manner, a pair of first insertion holes are formed in the first storage rack, and a freezing storage pipe is placed in the first storage rack; the second storage unit comprises a rotary table rotatably arranged on the inner side of the tank body, a support fixed on the rotary table and a second storage rack arranged on the support in a sliding manner along the radial direction, a pair of second jacks are arranged on the second storage rack, and a freezing pipe is placed in the second storage rack; the extraction unit comprises a supporting plate, a guide block arranged on the supporting plate in a sliding mode, a main rod arranged on the guide block in a sliding mode, a locking device movably arranged on the main rod, and a pair of supporting rods fixed on the locking device. The device can be convenient for the preservation of biological sample.
Description
Technical Field
The invention relates to the technical field of biological cell freezing, in particular to a biological cell freezing device convenient to search.
Background
In the prior art, when biological cells are frozen and preserved, the biological cells are filled into a freezing tube, and then the freezing tube is placed in a liquid nitrogen tank. The temperature of liquid nitrogen in the liquid nitrogen tank is-196 ℃, so that a person can neither extend into the liquid nitrogen tank by hands to take out a biological sample nor can the person quickly find out the required biological cells. Particularly, when the height of the liquid nitrogen tank is large, it is more difficult to take out the biological cells located at the lowermost layer of the liquid nitrogen tank. Similarly, the biological cells should be stored for easy searching during extraction, and when the biological cells are placed in the liquid nitrogen tank, in addition to protecting the arms of the operator, the biological samples should be accurately placed at the positions to be stored. Therefore, there is a need for a conveniently located biological cell freezing device for facilitating storage and extraction of biological samples.
Disclosure of Invention
The invention aims to provide a biological cell freezing device convenient to search, which is used for facilitating the storage and extraction of a biological sample.
The technical scheme adopted by the invention for solving the technical problems is as follows: biological cell refrigerating plant convenient to seek, including a jar body, characterized by is fixed with the filler ring at the jar mouthful inner wall of jar body, still includes:
the first storage unit comprises a plurality of vertical rods which are fixed on the inner wall of the tank body and are arranged at equal intervals, and a plurality of first storage frames which are arranged between every two adjacent vertical rods in an up-and-down equal interval mode and slide along the radial direction of the tank body, wherein a pair of first jacks are arranged on the first storage frames, and freezing storage pipes are placed in the first storage frames;
the second storage unit comprises a rotary table rotatably arranged on the inner side of the tank body, a C-shaped support fixed on the rotary table, and a second storage rack arranged in a groove in the side wall of the support in a sliding manner along the radial direction of the tank body, wherein a pair of second jacks are arranged on the second storage rack, and a freezing pipe is placed in the second storage rack;
the extraction unit comprises a supporting plate, a guide block arranged on the supporting plate in a radial sliding mode, a main rod arranged on the guide block in an up-and-down sliding mode, a lifting spring arranged between the main rod and the guide block, a handle fixed to the top of the main rod, a locking device movably arranged on the main rod, a pair of supporting rods fixed to the locking device, and a circular groove for placing the supporting plate is formed in the top of a supporting ring.
Further, the cross section of montant is the toper, is equipped with first spout on the lateral wall about the montant, is equipped with the first slider with first spout sliding fit at the lateral wall of first storage frame.
Furthermore, first storage frame is fan-shaped structure, and first storage frame is hollow structure, is equipped with first baffle in the inner chamber of first storage frame, and first baffle is the "T" shape structure, is equipped with a plurality of first round holes that are used for inserting the cryopreserving pipe on first baffle.
Furthermore, the second storage frame is of a cuboid structure, a T-shaped second partition plate is arranged in an inner cavity of the second storage frame, and a plurality of second round holes for inserting freezing tubes are formed in the second partition plate.
Furthermore, a rotating shaft is arranged at the axis position of the tank body and is rotationally connected with the tank body, and a plurality of side plates are arranged on the side wall of the rotating shaft; the bottom of the rotary table is provided with a shaft hole which is contacted with the outer wall of the rotating shaft, and side holes which are matched with the side plates in an inserting way are arranged around the shaft hole.
Furthermore, a plurality of locking holes which are arranged at equal intervals up and down are formed in the main rod, and the locking holes are in one-to-one correspondence with a plurality of first storage frames or second storage frames which are arranged up and down on the same bus.
Furthermore, a sliding cavity is formed in the locking device, a sliding plate is arranged in the sliding cavity in a sliding mode, a locking pin is fixed on the sliding plate, a spring is arranged between the sliding plate and the inner wall of the sliding cavity, and the action end of the locking pin extends into the locking hole under the action of the spring to achieve relative fixation of the locking device and the main rod.
Furthermore, a pair of rod grooves are formed in the side wall of the upper portion of the main rod, synchronizing rods are hinged in the rod grooves, a pair of positioning grooves are formed in the top of the support, and when the synchronizing rods are arranged in the positioning grooves, the support and the supporting plate rotate synchronously.
The invention has the beneficial effects that: the conveniently-found biological cell freezing device provided by the invention can be used for conveniently storing and extracting the freezing tube, and the position of the freezing tube to be extracted can be quickly found during extraction.
Drawings
FIG. 1 is a schematic external view of the present invention;
FIG. 2 is a schematic diagram of the internal structure of a liquid nitrogen tank;
FIG. 3 is a cross-sectional view of a liquid nitrogen tank;
FIG. 4 is a top view of the first storage rack;
FIG. 5 is a front view of the first storage rack;
FIG. 6 is a top view of the first separator plate;
FIG. 7 is a schematic view of the assembly of the second storage rack with the turntable;
FIG. 8 is a bottom view of the turntable;
FIG. 9 is a front view of the second storage rack;
FIG. 10 is a top view of a second separator plate;
FIG. 11 is a schematic view of the external form of the extraction unit;
FIG. 12 is a cross-sectional view of the extraction unit;
FIG. 13 is a schematic view of the locking mechanism;
in the figure: 1 can body, 11 can openings, 12 end covers, 13 vertical rods, 14 first sliding grooves, 15 rotating shafts, 16 side plates, 17 supporting rings, 2 first storage frames, 21 first sliding blocks, 22 first partition plates, 23 first insertion holes, 24 first round holes, 3 rotating discs, 31 shaft holes, 32 side holes, 4 supports, 41 grooves, 42 second sliding grooves, 43 positioning grooves, 5 second storage frames, 51 second sliding blocks, 52 second partition plates, 53 second round holes, 54 second insertion holes, 6 supporting plates, 61 guide grooves, 62 main rods, 63 guide blocks, 64 handles, 65 synchronizing rods, 66 locking holes, 7 locking devices, 71 supporting rods, 72 sliding plates, 73 locking pins, 74 springs and 75 sliding cavities.
Detailed Description
As shown in fig. 1 to 13, the present invention mainly includes a tank 1, a first storage unit, a second storage unit, and an extraction unit, and the present invention will be described in detail below with reference to the drawings.
As shown in fig. 1, a tank body 1 is a liquid nitrogen tank in the prior art, a tank opening 11 is arranged at the top of the liquid nitrogen tank, and an end cover 12 is arranged on the tank opening. As shown in fig. 2, a plurality of vertical rods 13 uniformly arranged along the same circumference are fixed on the inner wall of the tank body, and the arrangement direction of the vertical rods is parallel to the axial direction of the tank body. The cross section of montant is the toper, and the main aspects and jar internal wall fixed connection of montant are equipped with first spout 14 on the lateral wall about the montant, and first spout on each montant is a plurality of for evenly setting up from top to bottom, and lies in two first spouts of same height and set up in pairs on the montant. The axis of the tank body is provided with a rotating shaft 15, the rotating shaft is rotatably connected with the bottom of the tank body, a damping part is arranged between the rotating shaft and the tank body, and the rotating shaft and the tank body cannot rotate freely under the action of the damping part. Three side plates 16 are fixed on the circular side wall of the rotating shaft and are uniformly arranged on the same circumference. A supporting ring 17 is fixed on the inner wall of the tank opening, and the supporting ring is of a circular ring structure.
Be equipped with a plurality of first storage frame 2 that equidistant setting from top to bottom between every two adjacent montants, as shown in fig. 4, fig. 5, first storage frame 2 is fan-shaped structure, and the inside cavity of first storage frame, and the tip terminal surface of first storage frame is uncovered, is equipped with first slider 21 on the lateral wall about first storage frame, first slider be used for with first spout sliding connection. A first partition plate 22 is arranged in the inner cavity of the first storage rack, the first partition plate is in a reverse T-shaped structure, two first insertion holes 23 are arranged on the front side surface of the first partition plate, as shown in fig. 6, a plurality of first circular holes 24 are arranged on the first partition plate, and the first circular holes are numbered in sequence.
During the use, the freezing tube that is equipped with biological sample is put in the first round hole interpolation of first baffle, then places first storage frame between two adjacent montants to make first slider arrange corresponding first spout in, accomplished the placing of first storage frame promptly (biological sample's storage). 8 vertical rods can be arranged on the inner wall of the tank body, so that 8 placing areas are defined by the 8 vertical rods. The upper surface of the supporting ring is provided with 8 first identification lines, and the first identification lines correspond to the placing areas one by one. The vertical rod and the first storage rack form a first storage unit.
Be equipped with the second memory cell in the inboard of first memory cell, as shown in fig. 2, fig. 7 and fig. 8, the second memory cell includes carousel 3, support 4 and sets up a plurality of second storage shelves 5 on the support, and the carousel is the plectane, at the bottom shaft hole 31 of carousel, is equipped with three side opening 32 around the shaft hole, stretches into the pivot in the shaft hole and makes the curb plate stretch into in the side opening during the use, and the carousel can be at the internal rotation of jar like this. The support is of a C-shaped structure and can be seen as being obtained by cutting the side wall of the cylinder. The side wall of the support is provided with a plurality of uniformly arranged grooves 41, the left inner wall and the right inner wall of each groove are provided with second sliding grooves 42, the grooves on the same arc line are arranged at equal intervals, and the grooves on the same bus are located on the same vertical line. A second storage rack is placed in the groove, as shown in fig. 9 and 10, the second storage rack is of a rectangular parallelepiped structure, the left and right side walls of the second storage rack are respectively provided with a second sliding block 51, and the second sliding block is connected with the second sliding groove in a sliding manner. The second storage rack is of a hollow structure, the front side face of the second storage rack is open, a second partition plate 52 is arranged in the inner cavity of the second storage rack, the second partition plate is of a T-shaped structure, a plurality of second round holes 53 are formed in the second partition plate, and the second round holes are numbered sequentially. Two second insertion holes 54 are provided on the front side of the second partition plate.
Each recess is numbered according to the order, during the use, places biological sample in freezing and deposits intraductally, will freeze and deposit the pipe and put into the second round hole, then place the second storage frame in the recess that corresponds. A plurality of second identification lines are arranged on the upper surface of the support, and the second identification lines are in the same row number with the grooves and correspond to the grooves one to one.
Among the prior art, often will freeze and deposit the pipe and put on freezing the frame of depositing, freeze the frame of depositing and then hang at jar internal wall, take out the biological sample in certain freezing the intraductal when needs, often need take out whole freezing the frame of depositing, then find corresponding freezing the pipe. At the moment, other freezing pipes are also taken out, so that the freezing of other freezing pipes is influenced, and the required freezing pipes are inconvenient to search. In addition, the axial line position of the tank body is hollow, and the quantity of the biological samples which can be stored in the tank body is small.
According to the invention, the number of the biological samples which can be stored in the tank body is increased by arranging the first storage unit and the second storage unit which are arranged inside and outside, so that liquid nitrogen resources are fully utilized. In addition, first memory cell includes a plurality of first storage framves that evenly set up along the face of cylinder, and second memory cell includes a plurality of second storage framves that evenly set up along the face of cylinder, freezes to deposit the pipe and places on first, second storage frame, can realize categorised the placing like this, is convenient for seek.
In order to extract the frozen pipes, as shown in fig. 11 to 13, the present invention further comprises an extraction unit, wherein the extraction unit comprises a circular support plate 6, a main rod 62 slidably connected to the support plate along a radial direction, a handle 64 disposed at the top of the main rod, and an adjustable locking device 7 disposed on the main rod, a circular groove is disposed at the top of the support ring, and the support plate is placed in the circular groove when in use, and can rotate relative to the support ring. The supporting plate is provided with a guide groove 61 which is arranged along the radial direction of the supporting plate, the outer wall of the upper part of the main rod is provided with a guide block 63, and the guide block is connected with the guide groove in a sliding way. The main rod and the guide block can move relatively, and the guide block can move 1-2mm on the main rod. In a natural state, the main rod is at the lowest position relative to the guide block under the self weight; pulling the handle upward may cause the main lever to be positioned at the highest position relative to the guide block. The outer wall of the upper part of the main rod is provided with a pair of rod grooves, the inside of each rod groove is hinged with a synchronizing rod 65, and the synchronizing rods can be moved out of the rod grooves or turned over into the rod grooves as required. The lower part of mobile jib is hollow, is equipped with a plurality of locking holes 66 that equidistant setting from top to bottom on the mobile jib, and the locking hole is with not the first or second storage frame one-to-one of co-altitude. Be equipped with locking ware 7 on the mobile jib, the locking ware is the ring annular structure, and locking ware inner wall and mobile jib outer wall contact are fixed with a pair of bracing piece 71 on the locking ware outer wall, and two bracing pieces are used for stretching into in first or the second jack, and then with first or the second storage frame jack-up. A sliding cavity 75 is arranged in the locking device, a sliding plate 72 is slidably mounted in the sliding cavity, a locking pin 73 is fixed on the sliding plate, a spring 74 is arranged between the sliding plate and the inner wall of the sliding cavity, and the action end of the locking pin penetrates through the sliding cavity and then extends into the inner side of the locking device under the action of the spring. When the locking pin stretches into the locking hole, the main rod and the locking device are relatively fixed. And the supporting plate is provided with scale marks, and after the scale marks are aligned with the first identification lines, the fact that the first storage rack on the locking device is at a vertical position required to be stored is shown.
A pair of positioning grooves 43 are formed in the upper surface of the support, the synchronizing rod is turned over to be in a horizontal state, then the synchronizing rod extends into the positioning grooves, and at the moment, the supporting plate and the support rotate synchronously.
The procedure for storing the vial on the first storage rack is described below:
(1) placing the cryopreservation tube filled with the biological sample in a first round hole of a first storage rack;
(2) adjusting the position of a locking device on the main rod, locking and fixing the locking device, and extending the support rod into the first inserting hole in the first storage rack in the step (1), wherein the first storage rack corresponds to the height position of the required storage position;
(3) turning the synchronous rod to a horizontal state;
(4) placing the supporting plate on the supporting ring, wherein the first storage rack to be stored corresponds to the notch of the support, rotating the handle to enable the scale lines on the supporting plate to be aligned with the first identification lines of the required storage positions on the supporting ring, and the first storage rack corresponds to the circumferential position of the required storage positions;
(5) moving the handle towards the side wall direction of the tank body to enable the first storage rack to move along with the main rod until the first storage rack extends into the space between the paired first sliding grooves;
(6) then, the handle is pressed downwards while the handle is moved reversely, and at the moment, the support rod can be moved out of the first jack until the main rod is reset; thus, the access to the frozen storage tubes on the first storage rack is realized.
The procedure for storing the vial on the second storage rack is described below:
(1) placing the cryopreservation tube filled with the biological sample in a second round hole of a second storage rack;
(2) adjusting the position of a locking device on the main rod, locking and fixing the locking device, and extending the support rod into a second jack in the second storage rack in the step (1), wherein the second storage rack corresponds to the height position of the required storage position;
(3) placing the supporting plate on the supporting ring, rotating the handle to enable the scale marks on the supporting plate to be aligned with a second identification line of a required storage position on the support, and enabling the second storage rack to correspond to the circumferential position of the required storage position;
(4) moving the handle towards the side wall direction of the tank body to enable the second storage rack to move along with the main rod until the second storage rack extends into the corresponding groove;
(5) then, the handle is pressed downwards while the handle is moved reversely, and at the moment, the support rod can be moved out of the second jack until the main rod is reset; thus, the access to the frozen storage tubes on the second storage rack is realized.
The extraction of the first magazine is described below:
(1) turning over the synchronous rod to be in a horizontal state, placing the supporting plate on the supporting ring, enabling the synchronous rod to be placed in the positioning groove, and rotating the handle to enable the scale lines on the supporting plate to be aligned with the first identification lines corresponding to the first storage rack to be extracted;
(2) the handle is pressed downwards, and the handle is moved towards the side wall of the tank body until the support rod extends into the first jack of the first storage rack;
(3) withdrawing the downward acting force on the handle, and picking the first storage rack by the support rod at the moment; then the handle is moved reversely, so that the first storage rack moves along with the main rod and is moved out of the placing area;
(4) and lifting the handle to enable the supporting plate, the main rod, the locking device and the first storage rack to be moved out of the tank body.
The extraction of the second magazine is described below:
(1) placing the supporting plate on the supporting ring, and rotating the handle to enable the scale marks on the supporting plate to be aligned with second identification lines corresponding to a second storage rack to be extracted;
(2) the handle is pressed downwards, and the handle is moved towards the side wall of the tank body until the support rod extends into a second jack of the second storage rack;
(3) withdrawing the downward acting force on the handle, and picking the second storage rack by the support rod; then the handle is moved reversely, so that the second storage rack moves along with the main rod and is moved out of the placing area;
(4) and lifting the handle to enable the supporting plate, the main rod, the locking device and the second storage rack to be moved out of the tank body.
Claims (6)
1. Biological cell refrigerating plant convenient to seek, including a jar body, characterized by is fixed with the filler ring at the jar mouthful inner wall of jar body, still includes:
the first storage unit comprises a plurality of vertical rods which are fixed on the inner wall of the tank body and are arranged at equal intervals, and a plurality of first storage frames which are arranged between every two adjacent vertical rods in an up-and-down equal interval mode and slide along the radial direction of the tank body, wherein a pair of first jacks are arranged on the first storage frames, and freezing storage pipes are placed in the first storage frames;
the second storage unit comprises a rotary table rotatably arranged on the inner side of the tank body, a C-shaped support fixed on the rotary table, and a second storage rack arranged in a groove in the side wall of the support in a sliding manner along the radial direction of the tank body, wherein a pair of second jacks are arranged on the second storage rack, and a freezing pipe is placed in the second storage rack;
the extraction unit comprises a supporting plate, a guide block arranged on the supporting plate in a sliding manner along the radial direction, a main rod arranged on the guide block in a sliding manner up and down, a lifting spring arranged between the main rod and the guide block, a handle fixed at the top of the main rod, a locking device movably arranged on the main rod, and a pair of supporting rods fixed on the locking device, wherein a circular groove for placing the supporting plate is formed at the top of the supporting ring; a plurality of locking holes which are arranged at equal intervals up and down are arranged on the main rod, and the locking holes correspond to a plurality of first storage racks or second storage racks which are arranged up and down on the same bus one by one; the side wall on the upper part of the main rod is provided with a pair of rod grooves, the rod grooves are internally hinged with synchronizing rods, the top of the bracket is provided with a pair of positioning grooves, and when the synchronizing rods are arranged in the positioning grooves, the bracket and the supporting plate rotate synchronously.
2. The easy-to-find biological cell freezing device as claimed in claim 1, wherein the vertical rod has a tapered cross section, the left and right side walls of the vertical rod are provided with first sliding grooves, and the side wall of the first storage rack is provided with first sliding blocks slidably engaged with the first sliding grooves.
3. The easy-to-find biological cell freezing device according to claim 1, wherein the first storage rack is a fan-shaped structure, the first storage rack is a hollow structure, a first partition plate is arranged in the inner cavity of the first storage rack, the first partition plate is a reverse T-shaped structure, and a plurality of first round holes for inserting the freezing tubes are arranged on the first partition plate.
4. The apparatus for freezing biological cells for facilitating searching of claim 3, wherein the second storage rack has a rectangular parallelepiped shape, a second partition plate having a "T" shape is disposed in the inner cavity of the second storage rack, and a plurality of second circular holes for inserting the freezing tubes are disposed on the second partition plate.
5. The easy-to-find biological cell freezing device of claim 1, wherein a rotating shaft is provided at an axial position of the tank body, the rotating shaft is rotatably connected with the tank body, and a plurality of side plates are provided on side walls of the rotating shaft; the bottom of the rotary table is provided with a shaft hole which is contacted with the outer wall of the rotating shaft, and side holes which are matched with the side plates in an inserting way are arranged around the shaft hole.
6. The easy-to-find biological cell freezing device as claimed in claim 1, wherein a sliding chamber is provided in the locking device, a sliding plate is slidably mounted in the sliding chamber, a locking pin is fixed on the sliding plate, a spring is provided between the sliding plate and the inner wall of the sliding chamber, and an action end of the locking pin extends into the locking hole under the action of the spring to realize the relative fixation of the locking device and the main rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962030.3A CN110583628B (en) | 2019-10-11 | 2019-10-11 | Biological cell refrigerating device convenient to search |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910962030.3A CN110583628B (en) | 2019-10-11 | 2019-10-11 | Biological cell refrigerating device convenient to search |
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| Publication Number | Publication Date |
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| CN110583628A CN110583628A (en) | 2019-12-20 |
| CN110583628B true CN110583628B (en) | 2021-08-24 |
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| CN201910962030.3A Active CN110583628B (en) | 2019-10-11 | 2019-10-11 | Biological cell refrigerating device convenient to search |
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| CN115176797B (en) * | 2022-07-14 | 2023-12-22 | 安徽禾庚生物技术有限公司 | Biological sample preservation extractor |
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