CN106404511A - Sampling and freezing apparatus for biological sample - Google Patents
Sampling and freezing apparatus for biological sample Download PDFInfo
- Publication number
- CN106404511A CN106404511A CN201610777151.7A CN201610777151A CN106404511A CN 106404511 A CN106404511 A CN 106404511A CN 201610777151 A CN201610777151 A CN 201610777151A CN 106404511 A CN106404511 A CN 106404511A
- Authority
- CN
- China
- Prior art keywords
- cryopreservation tube
- support
- fixed
- clamping
- motor
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
Abstract
The invention provides a sampling and freezing apparatus for a biological sample to realize intelligent rapid sampling and short-term cryopreservation of the sample. A cryopreservation tube storage rack arranged on a frame realizes storage of a cryopreservation tube; a clamping assembly is arranged to clamp the cryopreservation tube on a cryopreservation tube storage assembly; the cryopreservation tube is transferred to a cutting driving assembly under the action of a transferring assembly; a locking lever on the cutting driving assembly can realize locking clamping of the cryopreservation tube; then a cryopreservation tube rotating mechanism drives the cryopreservation tube to carry out rotary movement, a cutter arranged on the opening end of the cryopreservation tube carries out rotary cutting on the biological sample, the cryopreservation tube moves downward under the driving of a cylinder, and thus, the cut biological sample is stored in the cryopreservation tube; and a cryopreservation assembly on the frame can realize short-term cryopreservation of the cryopreservation tube so as to maintain the activity of the sample. The sampling and freezing apparatus provided by the invention realizes high-efficiency sampling of the biological sample; and manual direct operation is not needed in operation of the apparatus, so labor force is alleviated.
Description
Technical field
The technical field the present invention relates to biological sample is drawn materials, specifically a kind of integrates the biology drawn materials and freeze
The quick sampling device of sample.
Background technology
Medical science is typically drawn materials to target sample by operating theater instruments, then manual transfer completes to cutting pipe again
Draw materials the preservation of sample.In general, from sample, to completing, the preservation required time is shorter in vitro, and the infringement to sample is fewer.
Conventional mode of drawing materials, needs the cutting in layer with operating equipment, then the sample of needs is cut into suitable size, respectively
Put into the cryopreservation tube of routine, the sample running into frost also needs to wait sample to thaw.Under aforesaid way, due to complex operation, institute
Arrive that the time completing to preserve is longer so that sample is in vitro, be very unfavorable for the draw materials quality of sample of guarantee.
Content of the invention
The refrigerating plant it is an object of the invention to provide biological sample is drawn materials, for realizing the intellectuality of sample is quickly taken
The short time of material and sample is frozen.
The present invention solves its technical problem and is adopted the technical scheme that：Biological sample is drawn materials refrigerating plant, it is characterized in that,
It includes frame, cryopreservation tube storage rack, conduit saddle, cryopreservation tube, transfer assembly, clamping assembly and cutting drive assembly, in described machine
Frame is provided with cryopreservation tube storage rack, and described cryopreservation tube storage rack mainly includes base, support tube, top cover, lift cylinder, connection
Bar, lifting disk and sucker, described base is fixed in frame, is rotatablely equipped with support tube at the top of described base, described
Base is provided with the rotary drive mechanism driving support tube rotation；It is provided with top cover at the top of described support tube, in described top cover
Bottom be provided with lift cylinder, some connecting rods are provided with the piston rod of described lift cylinder, in the outer wall of described support tube
It is provided with some and one-to-one bar hole of connecting rod, the end of described connecting rod extend in bar hole, in described support
The outside of cylinder is provided with the lifting disk of annular, and the end of described connecting rod is fixed on the inwall of lifting disk, in described lifting disk
Bottom some suckers being uniformly arranged are provided with same circumference, sucker by negative-pressure adsorption principle adsorb cryopreservation tube, rotation
The angle that support tube of the every action of drive mechanism rotates is 360 degree of numbers divided by sucker；
Described cryopreservation tube is the uncovered cylindrical hollow configuration in upper end, is provided with annular in the opening end of described cryopreservation tube
Cutter, are provided with some uniform cutting teeths on same circumference at the effect end of described cutter, in the envelope of described cryopreservation tube
Mouthful end be provided with some to locking hole, in the opening end screw thread of described cryopreservation tube, pipe cap is installed；
It is provided with transfer assembly in the end of described cryopreservation tube storage rack, described transfer assembly mainly includes first support, cunning
Plate, translation mechanism, second support, turnover driving mechanism and support bar, described first support is fixed in frame, described first
Support is provided with slide plate and drives slide plate to do the translation mechanism of linear reciprocating motion in the horizontal plane, is provided with described slide plate
Second support, is rotatablely equipped with upset worm gear and the upset worm screw of engagement cooperation, described upset worm screw in described second support
Setting coaxial with driven wheel of differential, described driven wheel of differential engages cooperation with drive bevel gear, and described drive bevel gear is fixed on
On the output shaft of upset rotating motor, described upset motor, drive bevel gear, driven wheel of differential, upset worm gear and upset worm screw structure
Become turnover driving mechanism；The outer wall of described upset worm gear is provided with a pair of support rods, the every action of upset motor once supports
Bar swings 180 degree in vertical plane；
Clamping assembly is fixed with described support bar, described clamping assembly mainly include the 3rd support, the first fork,
Two forks, first clamping plate, second clamping plate, cam and separation motor, described 3rd support is fixed on support bar, and support bar is located at
During the extreme position of both sides, the 3rd support is located in horizontal plane, is vertically installed with jointed shaft, described on described 3rd support
Hinged on jointed shaft the first fork and the second fork are installed, described first fork is fixed with first clamping plate, described
It is fixed with second clamping plate on two forks, first, second clamping plate is arc-shaped structure, be provided with the inner side of first, second clamping plate
Flexible pad, is provided with spring between first, second fork, and first, second clamping plate surrounds clamping cryopreservation tube under the action of the spring
Circular clamp chamber；Described 3rd support is provided with and separates motor, be provided with cam in the described output end separating motor, described
Cam is located between first, second fork and is provided with a pair raised, described raised and first, second fork on the outer wall of cam
The elastic force of spring is overcome so that first, second clamping plate is separated from each other after contact；
Cutting drive assembly is provided with described frame, described cutting drive assembly mainly include cylinder, transverse arm, housing,
Clamping disk, cryopreservation tube rotating mechanism and cryopreservation tube lockable mechanism, described cylinder is fixed in frame, in the piston rod of described cylinder
On be fixed with transverse arm, be provided with housing in the bottom of described transverse arm, in the inner rotation of described housing, clamping disk be installed, described
Clamping disk is provided with a pair of chute, is slidably fitted with locking rod in described chute, is fixed with lock in the lower end of described locking rod
Clamping, can achieve the clamping to cryopreservation tube, is provided with locking on described clamping disk when two lockings are buckled in paired locking hole
Motor, is provided with runner in the output end of described locking motor, is fixed with crank on described runner, in described crank and locking rod
Between be provided with connecting rod, pass through during locking motor action crank and connecting rod drive two locking rods synchronous closer or far from described lock
Only motor, runner, crank, connecting rod, locking rod and locking are buckled and are constituted lockable mechanism；It is provided with driving clamping disk in described housing
The cryopreservation tube rotating mechanism rotating；
Frozen assembly is additionally provided with described frame, described frozen assembly mainly includes box body, ice cube, dividing plate and pipe support,
Described box body is provided with lid, is provided with four dividing plates in the inner side of described box body, encloses between described dividing plate and the inwall of box body
Become to place the cavity of ice cube, be provided with pipe support in the inner side of dividing plate, some jacks inserting cryopreservation tube are provided with described pipe support.
Further, described rotary drive mechanism mainly includes electric rotating machine, swing pinion and gear ring, in described base
Top is provided with mounting groove, is provided with electric rotating machine in described mounting groove, is fixed with rotary teeth in the output end of described electric rotating machine
Wheel, described swing pinion is engaged with the gear ring being fixed on support tube inwall.
Further, described translation mechanism mainly includes translation motor, tooth bar and shiftable gear, and described translation motor is fixed
In first support, described tooth bar is fixed on slide plate, and described shiftable gear is fixed on output end and the tooth bar of translation motor
Engagement.
Further, described cryopreservation tube rotating mechanism mainly includes cutting motor, the first cutting gear and the second cutting teeth
Wheel, described first cutting gear is fixed in rotating shaft, is fixed with seal closure, described rotating shaft is solid with seal closure on described clamping disk
Even, it is provided with cutting motor in described inner walls, described second cutting gear engages and is fixed on cutting with the first cutting gear
The output end of motor.
Further, described crank is " one " character form structure.
Further, with the locking hole of cryopreservation tube wall contacts at arc transition.
Further, it is provided with bearing in described mounting groove, described support tube is fixed in the inner ring of bearing.
Further, described box body, dividing plate and lid are stainless steel part.
Further, it is provided with end cap in the lower end of described housing, described end cap is fixed with bearing, described clamping disk is solid
It is scheduled in the inner ring of bearing, the elongated slot avoiding locking rod is provided with described end cap.
The invention has the beneficial effects as follows：The biological sample that the present invention provides is drawn materials refrigerating plant, it is possible to achieve to cryopreservation tube
Deposit, when needing to carry out sample to draw materials, cryopreservation tube can move under the driving of rotary drive mechanism, to be in working position
Put.The setting of clamping assembly can clamp the cryopreservation tube on cryopreservation tube storage rack, turns cryopreservation tube in the presence of transfer assembly
Move at cutting drive assembly, the locking rod in cutting drive assembly can achieve the locking clamping to cryopreservation tube, then cryopreservation tube
Rotating mechanism drives cryopreservation tube to rotate, and now the cutter of cryopreservation tube opening end carry out circumgyration incision to biological sample,
Meanwhile under the drive of cylinder, cryopreservation tube moves down, and then the biological sample under cutting is stored in cryopreservation tube.Frozen total
The setting becoming, can carry out of short duration Cord blood to cryopreservation tube.The present invention can efficiently realize biological sample is drawn materials, and work
Manually need not directly operate when making, liberate manpower.
Brief description
Fig. 1 is the schematic diagram of the present invention；
Fig. 2 is the appearance schematic diagram of cryopreservation tube storage rack；
Fig. 3 is the schematic internal view of cryopreservation tube storage rack；
Fig. 4 is the graphics of cryopreservation tube；
Fig. 5 is the schematic diagram of cryopreservation tube and cutter；
Fig. 6 is the sectional view of cryopreservation tube；
Fig. 7 is the sectional view of pipe cap；
Fig. 8 is transfer assembly and the schematic diagram of clamping assembly；
Fig. 9 is the driving schematic diagram of slide plate；
Figure 10 is the schematic diagram of clamping assembly；
Figure 11 is schematic diagram when first, second clamping plate opens；
Figure 12 is the turnover driving mechanism schematic diagram of clamping assembly；
Figure 13 is the schematic diagram of cutting drive assembly；
Figure 14 is the schematic diagram of cryopreservation tube rotating mechanism；
Figure 15 is the schematic diagram of cryopreservation tube lockable mechanism；
Figure 16 is the scheme of installation of locking rod；
Figure 17 is the schematic diagram of frozen assembly；
Figure 18 is first or the schematic diagram of second clamping plate；
In figure：1 frame；
2 cryopreservation tube storage racks, 21 bases, 211 mounting grooves, 22 support tubes, 221 bar holes, 23 gear rings, 231 swing pinions,
232 electric rotating machines, 24 top covers, 25 lifting disks, 251 connecting rods, 26 lift cylinders, 261 piston rods, 27 suckers；
3 is hinged；
4 cryopreservation tubes, 41 external screw threads, 42 installing holes, 43 locking holes, 431 arc transition, 44 pipe caps, 45 cutter, 451 cut
Cut tooth；
5 transfer assemblies, 51 first supports, 511 chutes, 52 slide plates, 521 tooth bars, 522 shiftable gears, 523 translation motor,
53 second supports, 54 upset worm gears, 541 upset worm screws, 542 driven wheels of differential, 543 drive bevel gear, 544 upset motors, 55
Support bar；
6 clamping assemblies, 60 the 3rd supports, 61 first forks, 611 first fixed columns, 62 second forks, 621 second is fixing
Post, 63 first clamping plates, 64 second clamping plates, 65 springs, 66 separate motor, 67 cams, and 68 is raised；
7 cutting drive assembly, 71 feeding cylinders, 72 transverse arms, 73 housings, 74 clamping disks, 741 seal closures, 742 chutes, 743
Locking motor, 744 runners, 745 cranks, 746 connecting rods, 75 rotating shafts, 751 first cutting gears, 752 second cutting gears, 753 cut
Cut motor, 76 end caps, 761 elongated slots, 77 locking rods, 771 limited blocks, 78 locking buttons, 79 bearings；
8 frozen assemblies, 81 box bodys, 82 dividing plates, 83 ice cubes, 84 pipe supports, 85 jacks；
9 flexible pad.
Specific embodiment
As shown in Fig. 1 to Figure 18, the invention mainly comprises frame 1, cryopreservation tube storage rack 2, cryopreservation tube 4, transfer assembly 5,
Clamping assembly 6, cutting drive assembly 7 and frozen assembly, describe the present invention below in conjunction with the accompanying drawings.
As shown in figure 1, frame 1 is the support member of the present invention, it is provided with cryopreservation tube storage rack 2 at the top of frame.
Cryopreservation tube storage rack：
As shown in Figure 2 and Figure 3, cryopreservation tube storage rack mainly include base 21, support tube 22, gear ring 23, swing pinion 231,
Electric rotating machine 232, top cover 24, lifting disk 25, lift cylinder 26 and sucker 27, base is fixed in frame, and base is cryopreservation tube
The support member of storage rack, is provided with mounting groove 211 at the top of base, is provided with support tube 22 above base, and support tube is
Columnar structured, it is fixed with bearing 79 on the top of mounting groove, the lower end of support tube is fixed in the inner ring of bearing.Prop up for driving
The rotation of support cylinder, is provided with electric rotating machine 232 in mounting groove, is provided with swing pinion 231, rotary teeth in the output end of electric rotating machine
Wheel is engaged with gear ring 23, and gear ring is fixed on the inwall of support tube.Electric rotating machine, swing pinion and gear ring constitute driving and support
The rotary drive mechanism of cylinder rotation.It is provided with the top cover 24 of circle at the top of support tube, be provided with lifting at the center of cap base
Cylinder 26, is fixed with some connecting rods 251 on the piston rod 261 of hoist cylinder, and some connecting rods are uniform on same circumference
Distribution, and connecting rod, along radial setting, is provided with some bar holes 221 on the outer wall of support tube, the end of connecting rod extend into
In strip hole, it is provided with the lifting disk 25 of annular in the outside of support tube, the one end of the connecting rod being placed in bar hole is fixed on
On the inwall of lifting disk.So realize the connection of lifting disk and the piston rod of lift cylinder by connecting rod, at the bottom of lifting disk
Portion is provided with some suckers 27, and some suckers are uniformly distributed on same circumference, and sucker is adsorbed frozen by the principle of negative-pressure adsorption
Pipe.When depositing cryopreservation tube, cryopreservation tube is placed on sucker, often drives electric rotating machine action once, the angle that support tube turns over
For 360 degree of numbers divided by sucker.
Cryopreservation tube：
As shown in figure 4, cryopreservation tube 4 is the uncovered cylindrical hollow configuration in upper end, on the outer wall of the opening end of cryopreservation tube
It is provided with external screw thread 41, as shown in fig. 6, be provided with installing hole 42 in the inner side of the opening end of cryopreservation tube, in the sealing end of cryopreservation tube
Outer wall be provided with some to locking hole 43, locking hole is blind hole, the locking hole one end arc transition with cryopreservation tube wall contacts
431, as shown in figure 5, screw thread is provided with cutter 45 in installing hole, cutter are cirque structure, in the effect of cutter
End is provided with some cutting teeths 451 equally distributed on same circumference, and cutting teeth is triangular structure, and cutting teeth is used for cutting life
Thing sample.It is provided with pipe cap 44 in the opening end of cryopreservation tube, as shown in fig. 7, pipe cap is the uncovered cylindrical structural in lower end, in pipe cap
Inner side be provided with internal thread.
Transfer assembly：
As shown in Figure 8, Figure 9, transfer assembly mainly includes first support 51, slide plate 52, translation mechanism, second support 53 and
Turnover driving mechanism, first support 51 is the support member of transfer assembly, and first support is U-shaped structure, in first support
Wall is provided with chute 511, is provided with slide plate 52 in the inner side of first support, slide plate is connected with slide, in slide plate and first
It is provided with translation mechanism, translation mechanism mainly includes tooth bar 521, shiftable gear 522 and translation motor 523, and tooth bar is fixed between frame
In the bottom of slide plate, it is provided with translation motor 523 in the lower section of slide plate, be fixed with shiftable gear 522 in the output end of translation motor,
Shiftable gear engages cooperation with tooth bar, drives moving back and forth of slide plate by translation mechanism.It is fixed with second at the top of slide plate
Support 53, second support is the cuboid structure of upper end window, as shown in figure 12, is rotatablely equipped with the inner side of second support
Upset worm gear 54, is rotatablely equipped with upset worm screw 541 in the inner side of described second support, and upset worm screw is engaged with upset worm gear,
The worm shaft of upset worm screw is fixed with driven wheel of differential 542, is fixed with upset motor 544 in the bottom of second support,
The output end of upset motor is fixed with the drive bevel gear 543 engaging with driven wheel of differential, upset motor, upset worm gear, upset
Worm screw, driven wheel of differential and drive bevel gear constitute turnover driving mechanism.The outer wall of upset worm gear is fixed with two
Strut 55, one end of two support bars is fixedly connected with upset worm gear, is provided with clamping assembly 6 in the other end of two support bars.
Clamping assembly：
As shown in Figure 10, clamping assembly mainly includes the 3rd support 60, the first fork 61, the second fork 62, first clamping plate
63rd, second clamping plate 64, spring 65, separation motor 66 and cam 67, the 3rd support is the support member of clamping assembly, clamps assembly
It is fixed on support bar by the 3rd support.3rd support is L-shaped structure, is provided with the first fork 61 and second on the 3rd support
Fork 62, the installation end of the first fork is connected by jointed shaft hinged with the installation end of the second fork 3, for avoid the first fork with
Interference each other when second fork swings, in the side of the first fork elongated slot relative with the second fork, in swing process
In the second fork can extend in the elongated slot on the first fork.It is fixed with the first clamping plate of circular arc in the free end of the first fork
63, it is fixed with the second clamping plate 64 of circular arc in the free end of the second fork, made by the cooperation of first clamping plate and second clamping plate
With realizing the clamping to cryopreservation tube.For avoiding first, second clamping plate to deform frozen pipe clamp, as shown in figure 18, first, second
The inner side of clamping plate is provided with flexible pad 9, flat to avoid cryopreservation tube to be pressed from both sides.
It is fixed with the first fixed column 611 at the top of the free end of the first fork, at the top of the free end of the second fork
It is fixed with the second fixed column 621, be provided with spring 65 between the first fixed column and the second fixed column, under the action of the spring
First, second clamping plate surrounds the circular clamp chamber of clamping cryopreservation tube.For drive first, second fork swing sideward dynamic make first,
Second fork is separated from each other, and is provided with and separates motor 66 between the first fork and the second fork, separates motor and is fixed on the 3rd
On frame, it is provided with cam 67 in the output end separating motor, the outer wall of cam is provided with raised 68, the setting of cam makes cam
On there are two peaks, raised outer wall is smooth.As shown in figure 11, separation motor is driven to rotate the projection so that on cam
Gradually contact with first, second fork, under the squeezing action of cam, now overcome the elastic force of spring so that first, second
Fork is separated from each other, and then first clamping plate is separated with second clamping plate, drives slide plate near jelly now by translation mechanism
Deposit a side shifting at pipe storage rack place, until cryopreservation tube is placed between first, second clamping plate, subsequently drive separation motor to turn
Dynamic, until the projection on cam is separated with first, second fork, now first clamping plate and second clamping plate mating reaction is by cryopreservation tube
Clamping, and the opening end of cryopreservation tube is upward.Subsequently, drive the translation motor reverse rotary motion of output, now slide plate to away from
One side shifting of cryopreservation tube storage rack；After first, second clamping plate is from the moved out from beneath of first support, upset motor action, make
The 3rd support upset 180 degree, now the opening end of cryopreservation tube is down.For realizing the clamping again to cryopreservation tube, realize opposite
Drawing materials of thing sample, is provided with cutting drive assembly 7 on the right side of transfer assembly.
Cutting drive assembly：
As shown in FIG. 13 to 15, cutting drive assembly mainly includes feeding cylinder 71, transverse arm 72, housing 73, cryopreservation tube
Rotating mechanism and cryopreservation tube lockable mechanism, the cylinder barrel of the vertical feeding cylinder 71 placed is fixed in frame, in feeding cylinder
Piston rod free end is fixed with transverse arm 72, and transverse arm is located in horizontal plane, and the installation end of transverse arm is connected with the piston rod of cylinder, in horizontal stroke
The bottom of the free end of arm is provided with housing 73, and as shown in figure 14, housing is the uncovered cylindrical hollow configuration in lower end, in housing
The bottom in chamber is provided with clamping disk 74, and as shown in Figure 15, Figure 16, clamping disk is circular configuration, is provided with a pair of chute on clamping disk
742, two chutes are with regard to the center of circle Central Symmetry of clamping disk.It is slidably fitted with locking rod 77 in chute, locking rod is along clamping disk
Axially arranged, the bottom of locking rod is located at the lower section of clamping disk, is fixed with locking button 78 in the bottom of locking rod, locking button is
Cylindrical bar, locking button and locking rod are vertically arranged, and one end of locking button is connected with locking rod, and the other end of locking button is hemisphere
Shape.Be fixed with the limited block 771 above clamping disk at the top of locking rod, the setting of limited block can prevent locking rod from
Drop on clamping disk.It is provided with locking motor 743 at the center of clamping disk, is provided with runner 744 in the output end of locking motor, turning
The top of wheel is fixed with crank 745, and crank is in-line bar, and the middle part of crank is fixed on the wheel shaft of runner, the one of crank
It is provided with connecting rod 746 between end and one of limited block, between the other end and second limited block of crank, be provided with connecting rod
746, connecting rod and crank hinged 3, connecting rod is hinged with limited block.During the work of locking motor, drive the rotation of runner and crank, then
Slip in chute for the locking rod is driven by connecting rod, and two locking rods are simultaneously away from runner or simultaneously near runner.Locking electricity
Machine 734, runner 744, crank 745, connecting rod 746, locking rod, clamping disk and locking are buckled and are constituted cryopreservation tube lockable mechanism.
Be fixed with seal closure 741 at the top of clamping disk, seal closure covers on the outside of cryopreservation tube lockable mechanism, seal closure with
Clamping disk is fixedly connected.It is fixed with rotating shaft 75 at the top of seal closure, axis of rotation is arranged on the inside of housing, solid in rotating shaft
Surely there is the first cutting gear 751, be provided with cutting motor 753 at the top of housing, be fixed with and the in the output end of cutting motor
Second cutting gear 752 of one cutting gear engagement, cutting motor, rotating shaft, the first cutting gear and the second cutting gear are constituted
Cryopreservation tube rotating mechanism.
For ease of installing, realizing sealing, it is provided with end cap 76 in the bottom of housing, end cap is provided with and holds locking rod movement
Elongated slot 761.Bearing 79 is fixed with end cap, clamping disk is fixed in the inner ring of bearing.Clamping assembly upset 180 degree is simultaneously being put down
After in the presence of telephone-moving structure, cryopreservation tube is located at the underface of clamping disk, cylinder piston rod is retracted one section, now locking hole and locking
Button is located in the same horizontal plane, and drives cutting motor low speed action so that after locking button alignd with locking hole, locking motor action
Locking button is extend in locking hole, now just achieves the clamping to cryopreservation tube, subsequently the in the presence of separating motor
First, separately, in the presence of translation mechanism, clamping assembly, to the side shifting near cryopreservation tube storage rack, then turns over the second fork
Rotating motor action makes to clamp after assembly reversely rotates 180 degree and resets.Finally, cutting motor highly rotates, simultaneously the work of cylinder
Stopper rod is slowly retracted, and now the cutter of cryopreservation tube opening end carry out circumgyration incision to biological sample, cryopreservation tube rotation
Simultaneously while declining, so the biological sample cutting down just enters in cryopreservation tube, when cutting quantity reaches requirement, make cutting
Motor stopping action, now driving cylinder piston rod stretch out, cryopreservation tube is extracted from biological sample.Drive locking motor
So that locking rod is to the side shifting away from runner, now cryopreservation tube is gradually unclamped output counter rotational movement by locking button, when
After cryopreservation tube is removed from cutting drive assembly, just pipe cap can be arranged on cryopreservation tube, now cryopreservation tube can be put into frozen
Preserved in assembly.
Frozen assembly：
As shown in figure 17, frozen assembly mainly includes box body 81, dividing plate 82, ice cube 83 and pipe support 84, and box body is cuboid
The working of plastics of shape or stainless steel metal part, are provided with lid on box body, are provided with dividing plate 82 in the inner side of box body, dividing plate is provided with four
Individual and four dividing plates surround a square structure, surround airtight cavity between dividing plate and inboard wall of cartridge, place in the cavity
Ice cube 83, is placed with pipe support 84 in the inner side of dividing plate, and pipe support integrally becomes cuboid, is provided with some jacks 85 on pipe support,
Cryopreservation tube is inserted in jack.Cryopreservation tube is inserted in jack, covers the lid of box body, it is possible to achieve be of short duration low to cryopreservation tube
Temperature preserves.Lid, box body and dividing plate are working of plastics or stainless steel metal part.
Claims (9)
1. biological sample is drawn materials refrigerating plant, it is characterized in that, it includes frame, cryopreservation tube storage rack, conduit saddle, cryopreservation tube, transfer
Assembly, clamping assembly and cutting drive assembly, are provided with cryopreservation tube storage rack, described cryopreservation tube storage rack is main in described frame
Including base, support tube, top cover, lift cylinder, connecting rod, lifting disk and sucker, described base is fixed in frame, described
The top of base is rotatablely equipped with support tube, is provided with the rotary drive mechanism driving support tube rotation on described base；Institute
The top stating support tube is provided with top cover, is provided with lift cylinder in the bottom of described top cover, on the piston rod of described lift cylinder
It is provided with some connecting rods, some and one-to-one bar hole of connecting rod, described connection are provided with the outer wall of described support tube
The end of bar extend in bar hole, is provided with the lifting disk of annular, the end of described connecting rod in the outside of described support tube
It is fixed on the inwall of lifting disk, in the bottom of described lifting disk, some suckers being uniformly arranged is provided with same circumference, inhale
Disk adsorbs cryopreservation tube by negative-pressure adsorption principle, and the angle that support tube of the every action of rotary drive mechanism rotates is removed for 360 degree
Number with sucker；
Described cryopreservation tube is the uncovered cylindrical hollow configuration in upper end, is provided with the cutting of annular in the opening end of described cryopreservation tube
Knife, is provided with some uniform cutting teeths on same circumference at the effect end of described cutter, in the sealing end of described cryopreservation tube
Be provided with some to locking hole, in the opening end screw thread of described cryopreservation tube, pipe cap is installed；
It is provided with transfer assembly in the end of described cryopreservation tube storage rack, described transfer assembly mainly includes first support, slide plate, puts down
Telephone-moving structure, second support, turnover driving mechanism and support bar, described first support is fixed in frame, in described first support
It is provided with slide plate and drives slide plate to do the translation mechanism of linear reciprocating motion in the horizontal plane, described slide plate is provided with second
Support, described second support is rotatablely equipped with engagement cooperation upset worm gear and upset worm screw, described upset worm screw with from
The coaxial setting of dynamic bevel gear, described driven wheel of differential engages cooperation with drive bevel gear, and described drive bevel gear is fixed on upset
On the output shaft of rotating motor, described upset motor, drive bevel gear, driven wheel of differential, upset worm gear and upset worm screw constitute
Turnover driving mechanism；The outer wall of described upset worm gear is provided with a pair of support rods, support bar of the every action of upset motor exists
180 degree is swung in vertical plane；
Clamping assembly is fixed with described support bar, described clamping assembly mainly includes the 3rd support, the first fork, the second pendulum
Bar, first clamping plate, second clamping plate, cam and separation motor, described 3rd support is fixed on support bar, and support bar is located at both sides
Extreme position when the 3rd support be located at horizontal plane in, described 3rd support is vertically installed with jointed shaft, described hinged
Hinged on axle the first fork and the second fork are installed, described first fork is fixed with first clamping plate, described second pendulum
It is fixed with second clamping plate on bar, first, second clamping plate is arc-shaped structure, be provided with flexibility in the inner side of first, second clamping plate
Pad, is provided with spring between first, second fork, and first, second clamping plate surrounds the circle of clamping cryopreservation tube under the action of the spring
Shape holding chamber；Described 3rd support is provided with and separates motor, be provided with cam in the described output end separating motor, described cam
It is provided with a pair of projection between first, second fork and on the outer wall of cam, described projection is contacted with first, second fork
Overcome the elastic force of spring afterwards so that first, second clamping plate is separated from each other；
Cutting drive assembly is provided with described frame, described cutting drive assembly mainly includes cylinder, transverse arm, housing, clamping
Disk, cryopreservation tube rotating mechanism and cryopreservation tube lockable mechanism, described cylinder is fixed in frame, solid on the piston rod of described cylinder
Surely there is transverse arm, be provided with housing in the bottom of described transverse arm, in the inner rotation of described housing, clamping disk is installed, in described clamping
Disk is provided with a pair of chute, is slidably fitted with locking rod in described chute, is fixed with locking button in the lower end of described locking rod,
Can achieve the clamping to cryopreservation tube when two lockings are buckled in paired locking hole, locking motor be provided with described clamping disk,
It is provided with runner in the output end of described locking motor, described runner is fixed with crank, between described crank and locking rod
It is provided with connecting rod, pass through crank during locking motor action and connecting rod drives the synchronization of two locking rods closer or far from described locking electricity
Machine, runner, crank, connecting rod, locking rod and locking are buckled and are constituted lockable mechanism；It is provided with driving clamping disk and does in described housing and revolve
The dynamic cryopreservation tube rotating mechanism of transhipment；
Frozen assembly is additionally provided with described frame, described frozen assembly mainly includes box body, ice cube, dividing plate and pipe support, in institute
State box body and be provided with lid, be provided with four dividing plates in the inner side of described box body, surround between described dividing plate and the inwall of box body and put
Put the cavity of ice cube, be provided with pipe support in the inner side of dividing plate, some jacks inserting cryopreservation tube are provided with described pipe support.
2. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, described rotary drive mechanism mainly wraps
Include electric rotating machine, swing pinion and gear ring, be provided with mounting groove at the top of described base, in described mounting groove, be provided with electric rotating
Machine, is fixed with swing pinion, described swing pinion and the tooth being fixed on support tube inwall in the output end of described electric rotating machine
Circle engagement.
3. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, described translation mechanism mainly includes putting down
Move motor, tooth bar and shiftable gear, described translation motor is fixed in first support, and described tooth bar is fixed on slide plate, described
Shiftable gear is fixed on the output end of translation motor and is engaged with tooth bar.
4. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, described cryopreservation tube rotating mechanism is main
Including cutting motor, the first cutting gear and the second cutting gear, described first cutting gear is fixed in rotating shaft, in described folder
Hold and be fixed with seal closure on disk, described rotating shaft is connected with seal closure, is provided with cutting motor in described inner walls, described second cuts
Cut gear to engage and be fixed on the output end of cutting motor with the first cutting gear.
5. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, described crank is that " one " font is tied
Structure.
6. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, the locking with cryopreservation tube wall contacts
Arc transition at hole.
7. biological sample according to claim 2 is drawn materials refrigerating plant, it is characterized in that, is provided with axle in described mounting groove
Hold, described support tube is fixed in the inner ring of bearing.
8. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, described box body, dividing plate and lid are equal
For stainless steel part.
9. biological sample according to claim 1 is drawn materials refrigerating plant, it is characterized in that, is provided with end in the lower end of described housing
Lid, is fixed with bearing, described clamping disk is fixed in the inner ring of bearing on described end cap, is provided with avoidance lock on described end cap
The elongated slot of stopping bar.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610777151.7A CN106404511B (en) | 2016-08-31 | 2016-08-31 | Biological sample materials refrigerating plant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610777151.7A CN106404511B (en) | 2016-08-31 | 2016-08-31 | Biological sample materials refrigerating plant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106404511A true CN106404511A (en) | 2017-02-15 |
| CN106404511B CN106404511B (en) | 2018-10-09 |
Family
ID=58004066
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610777151.7A Active CN106404511B (en) | 2016-08-31 | 2016-08-31 | Biological sample materials refrigerating plant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106404511B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843453A (en) * | 2017-12-21 | 2018-03-27 | 上海泽泉科技股份有限公司 | Adjustable speed holds low temperature sample packing instrument |
| CN108269687A (en) * | 2018-02-05 | 2018-07-10 | 钱立文 | A kind of supply head of aluminium shell of capacitor cutting machine |
| CN108543971A (en) * | 2018-07-06 | 2018-09-18 | 合肥格骄电子科技有限公司 | A kind of boring and milling machine of optical, mechanical and electronic integration |
| CN110583628A (en) * | 2019-10-11 | 2019-12-20 | 山东山大附属生殖医院有限公司 | Biological cell refrigerating device convenient to search |
| CN111114101A (en) * | 2020-01-18 | 2020-05-08 | 温州职业技术学院 | Pressure adjusting device of flexible printing machine |
| CN114958585A (en) * | 2022-07-29 | 2022-08-30 | 太平洋康泰科学仪器（济南）有限公司 | Biological cell sample extraction manipulator |
| CN117705584A (en) * | 2024-02-05 | 2024-03-15 | 常州凯得新材料科技有限公司 | Device for aging test of polyethylene film |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687735A (en) * | 2005-04-23 | 2005-10-26 | 胡盛平 | Method for fabricating section of biologic tissue |
| WO2014147696A1 (en) * | 2013-03-18 | 2014-09-25 | 株式会社島津製作所 | Sample cooling device, autosampler provided with same, and sample cooling method |
| CN104316386A (en) * | 2014-10-22 | 2015-01-28 | 中国人民解放军总医院第一附属医院 | Device for performing freeze fracture on scanning electron microscope sample |
| CN105424403A (en) * | 2015-11-10 | 2016-03-23 | 舟山出入境检验检疫局综合技术服务中心 | Frozen-seafood sampling device for laboratory |
| CN105675369A (en) * | 2016-03-21 | 2016-06-15 | 广州深达生物制品技术有限公司 | Apparatus and method for treating biological slide sample |
-
2016
- 2016-08-31 CN CN201610777151.7A patent/CN106404511B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1687735A (en) * | 2005-04-23 | 2005-10-26 | 胡盛平 | Method for fabricating section of biologic tissue |
| WO2014147696A1 (en) * | 2013-03-18 | 2014-09-25 | 株式会社島津製作所 | Sample cooling device, autosampler provided with same, and sample cooling method |
| CN105324655A (en) * | 2013-03-18 | 2016-02-10 | 株式会社岛津制作所 | Sample cooling device, autosampler provided with same, and sample cooling method |
| CN104316386A (en) * | 2014-10-22 | 2015-01-28 | 中国人民解放军总医院第一附属医院 | Device for performing freeze fracture on scanning electron microscope sample |
| CN105424403A (en) * | 2015-11-10 | 2016-03-23 | 舟山出入境检验检疫局综合技术服务中心 | Frozen-seafood sampling device for laboratory |
| CN105675369A (en) * | 2016-03-21 | 2016-06-15 | 广州深达生物制品技术有限公司 | Apparatus and method for treating biological slide sample |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107843453A (en) * | 2017-12-21 | 2018-03-27 | 上海泽泉科技股份有限公司 | Adjustable speed holds low temperature sample packing instrument |
| CN107843453B (en) * | 2017-12-21 | 2024-01-23 | 上海泽泉科技股份有限公司 | Speed-adjustable handheld low-temperature sample split charging instrument |
| CN108269687A (en) * | 2018-02-05 | 2018-07-10 | 钱立文 | A kind of supply head of aluminium shell of capacitor cutting machine |
| CN108269687B (en) * | 2018-02-05 | 2019-10-01 | 钱立文 | A kind of supply head of aluminium shell of capacitor cutting machine |
| CN108543971A (en) * | 2018-07-06 | 2018-09-18 | 合肥格骄电子科技有限公司 | A kind of boring and milling machine of optical, mechanical and electronic integration |
| CN110583628A (en) * | 2019-10-11 | 2019-12-20 | 山东山大附属生殖医院有限公司 | Biological cell refrigerating device convenient to search |
| CN110583628B (en) * | 2019-10-11 | 2021-08-24 | 山东山大附属生殖医院有限公司 | Biological cell refrigerating device convenient to search |
| CN111114101A (en) * | 2020-01-18 | 2020-05-08 | 温州职业技术学院 | Pressure adjusting device of flexible printing machine |
| CN111114101B (en) * | 2020-01-18 | 2021-08-06 | 温州职业技术学院 | Pressure adjusting device of flexible printing machine |
| CN114958585A (en) * | 2022-07-29 | 2022-08-30 | 太平洋康泰科学仪器（济南）有限公司 | Biological cell sample extraction manipulator |
| CN117705584A (en) * | 2024-02-05 | 2024-03-15 | 常州凯得新材料科技有限公司 | Device for aging test of polyethylene film |
| CN117705584B (en) * | 2024-02-05 | 2024-04-05 | 常州凯得新材料科技有限公司 | Device for aging test of polyethylene film |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106404511B (en) | 2018-10-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106404511A (en) | Sampling and freezing apparatus for biological sample | |
| GB2556646A (en) | Vacuum juicer | |
| CN106289860B (en) | A kind of quick sampling device of biological sample | |
| CN106577633B (en) | Automate freezing of semen device | |
| CN107878801A (en) | A kind of wrapping machine for being conveniently replaceable packaging film | |
| CN207328918U (en) | Vacuum packing machine | |
| CN210455955U (en) | Preservation device of graphite alkene powder | |
| CN113150960B (en) | Medical clinical microbiological detection sampling device | |
| CN204503361U (en) | A kind of centrifuge | |
| CN112238967A (en) | Tank degassing device for aquatic product can production and implementation method thereof | |
| CN201250089Y (en) | Small single knife device for bottle capper | |
| CN209901515U (en) | Biological medium centrifugal equipment | |
| CN218654564U (en) | Fixing device is deposited to sample liquid nitrogen freezing | |
| CN203751067U (en) | Canister rib rolling machine | |
| CN105231794A (en) | Linkage mechanism used for upper bowl of single-cup juice extractor | |
| CN215504494U (en) | Steam sterilization cabinet for sterilization and disinfection of bovine serum production equipment | |
| CN107706322B (en) | Capacity series battery capping device | |
| CN110790206A (en) | Encapsulation equipment for food production | |
| CN110304598B (en) | Penicillin bottle cover opening device | |
| CN214937039U (en) | Safe and efficient frosting device for glass bottles | |
| CN113854282B (en) | Medical low-temperature preservation device | |
| CN113832002B (en) | A shake incubator for bacterial cultivation | |
| CN220596288U (en) | Medicine bottle taking device with high applicability | |
| CN220832356U (en) | Beer rack with protective structure | |
| CN212693348U (en) | Quantitative sampling device for isolation milk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |