CN107843453B - Speed-adjustable handheld low-temperature sample split charging instrument - Google Patents
Speed-adjustable handheld low-temperature sample split charging instrument Download PDFInfo
- Publication number
- CN107843453B CN107843453B CN201711395849.3A CN201711395849A CN107843453B CN 107843453 B CN107843453 B CN 107843453B CN 201711395849 A CN201711395849 A CN 201711395849A CN 107843453 B CN107843453 B CN 107843453B
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- China
- Prior art keywords
- shell
- push rod
- needle
- hollow shaft
- packaging machine
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000005070 sampling Methods 0.000 claims abstract description 44
- 238000004806 packaging method and process Methods 0.000 claims description 11
- 230000005540 biological transmission Effects 0.000 claims description 8
- 239000000523 sample Substances 0.000 abstract description 24
- 238000010257 thawing Methods 0.000 abstract description 8
- 239000012520 frozen sample Substances 0.000 abstract description 3
- 238000005553 drilling Methods 0.000 description 7
- 230000008014 freezing Effects 0.000 description 5
- 238000007710 freezing Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 3
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
- G01N1/08—Devices for withdrawing samples in the solid state, e.g. by cutting involving an extracting tool, e.g. core bit
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
Abstract
The invention provides a speed-adjustable handheld low-temperature sample split charging instrument, which comprises a shell and a sampling drill needle, wherein a hollow shaft and a driving mechanism for driving the hollow shaft to rotate are arranged in the shell; a push rod is further arranged in the shell, the top end of the push rod extends out of the top of the shell, and the bottom end of the push rod extends into the hollow shaft and contacts with the top of the drill needle inner core which also extends into the hollow shaft; the front side of shell is equipped with the spout, and the spout inboard is equipped with can follow the gliding slider of spout, still is equipped with on the spout and is used for locking the locking screw of slider position is equipped with on the push rod with slider matched with locking seat limits the upper limit of push rod through the cooperation of slider and locking seat and removes the position. The invention can conveniently carry out repeated sampling without freeze thawing on the frozen sample, and has the advantages of small volume, compact design and easy operation.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a speed-adjustable handheld low-temperature sample split charging instrument.
Background
Pathological diagnosis is a medical subject for studying the cause of disease, and sampling observation is a process that must be performed. After taking samples of human, animal and plant tissues in a laboratory, the samples are frozen and preserved at low temperature or ultra-low temperature, and when the samples are required to be used, the samples are frozen very firmly, and the sampling operation of the samples is very difficult. The conventional sampling method is thawing sampling or freezing and slicing, wherein thawing sampling requires thawing samples, repeated freezing and thawing of samples are required if sampling is performed at intervals, which can greatly affect the quality of the samples, while freezing and slicing generally uses a freezing and slicing machine for related operation, the sampling volume and the integrity of the samples are not easy to control, the volume of the device is large, and the operation steps are complicated.
Disclosure of Invention
In order to overcome the defects of the prior art, the invention provides the speed-adjustable handheld low-temperature sample split charging instrument which can conveniently perform repeated sampling without freeze thawing on frozen samples, and has the advantages of small volume, compact design and easy operation.
The technical scheme adopted for solving the technical problems is as follows: the utility model provides a handheld low temperature sample partial shipment appearance of adjustable speed, includes shell and sampling drill point, be equipped with cavity axle and drive this cavity axle pivoted actuating mechanism in the shell, the bottom of cavity axle stretches out the shell bottom, sampling drill point is connected with cavity axle interference fit, still is equipped with the drill point inner core that stretches out its top in the sampling drill point; a push rod is further arranged in the shell, the top end of the push rod extends out of the top of the shell, and the bottom end of the push rod extends into the hollow shaft and contacts with the top of the drill needle inner core which also extends into the hollow shaft; the front side of shell is equipped with the spout, and the spout inboard is equipped with can follow the gliding slider of spout, still is equipped with on the spout and is used for locking the locking screw of slider position, be equipped with on the push rod with slider matched with locking seat limits the upper limit of push rod through the cooperation of slider and locking seat and removes the position.
As a further preferred embodiment, the drive mechanism comprises a drive motor and a drive shaft connected with the drive motor, wherein the drive shaft and the hollow shaft are driven in a gear transmission or belt transmission mode.
As a further preferable implementation scheme, a control circuit board and a battery are arranged in the shell, the battery and the driving motor are respectively connected with the control circuit board, and a key which is connected with the control circuit board and used for controlling the start and stop of the driving motor and the rotating speed of the driving motor is also arranged on the front side surface of the shell. Preferably, the battery is a rechargeable battery, and the shell is provided with a charging interface connected with the battery.
As a further preferred embodiment, a needle abandoning device is arranged in the shell, the top end of the needle abandoning device extends out of the top of the shell, the bottom end of the needle abandoning device extends out of the bottom of the shell and is connected with the sampling probe, a mounting groove is arranged on the rear side surface of the shell, a protruding plate which is bent backwards and extends into the mounting groove is arranged on the needle abandoning device, a spring is also arranged in the mounting groove, one end of the spring is in contact connection with the inner bottom end of the mounting groove, and the other end of the spring is in contact connection with the protruding plate.
As a further preferable embodiment, the needle abandoning device is arranged behind the push rod, the top end of the needle abandoning device is provided with a pressing part bent to one side, and the needle abandoning device is also provided with a protruding strip bent forwards and matched with the locking seat.
As a further preferred embodiment, the housing is formed by combining an upper housing and a lower housing with each other.
As a further preferred embodiment, the upper half of the housing is a hand-held part, and the back surface of the housing is provided with a hook body which is convenient to hold and bends downwards.
As a further preferred embodiment, the locking seat is locked to the ejector rod by means of a jackscrew, the hollow shaft being connected to a bearing which is arranged on a fixed seat inside the housing.
As a further preferable embodiment, the top end of the push rod is provided with a pressing round table which is convenient to press.
The invention has the positive effects that:
(1) The invention can meet the requirements of users on repeated sampling of frozen samples without freeze thawing, sampling of sample target areas and the like, and has extremely high operation safety.
(2) The invention can efficiently treat frozen tissue and biological liquid samples, ensures the integrity of the samples to the maximum extent, and simultaneously, has standard sampling shape and controllable sampling volume, thereby optimizing experimental results.
(3) By maintaining the frozen state of the sample at ultralow temperature for sampling, the consistency of the quality of the original sample and the quality of the sampled sample is ensured, and any potential hazard to the sample caused by freeze thawing is eliminated.
(4) Unlike other frozen slicing machine and desk-top machine, the invention has small volume, compact and elegant design and can be easily stored and placed. The unique charging and single-key arrangement make the invention easier to operate, and save precious time for users.
Drawings
FIG. 1 is a schematic view of the front structure of a dispenser according to the present invention;
FIG. 2 is a schematic view of the back structure of the dispenser according to the invention;
FIG. 3 is a schematic view of the internal structure of the housing of the dispensing apparatus according to the present invention;
FIG. 4 is a schematic view of the structure of the sampling drill according to the present invention;
FIG. 5 is a schematic view of the structure of the needle-discarding device according to the present invention;
FIG. 6 is a schematic view of the hollow shaft of the present invention;
FIG. 7 is a schematic view showing an exploded structure of the locking screw and the slider according to the present invention;
fig. 8 is a schematic structural view of the locking seat according to the present invention.
Detailed Description
The preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.
Referring to fig. 1-8, a preferred embodiment of the present invention provides a speed-adjustable handheld low-temperature sample sub-packaging apparatus, which comprises a housing 101 and a sampling drill needle 401, wherein a hollow shaft 105 and a driving mechanism for driving the hollow shaft to rotate are arranged in the housing 101, the bottom end of the hollow shaft 105 extends out of the bottom of the housing, the sampling drill needle 401 is connected with the hollow shaft 105 in an interference fit manner, and a drill needle inner core 402 extending out of the top end of the sampling drill needle 401 is also arranged in the sampling drill needle 401; as shown in fig. 3, a push rod 301 is further disposed in the housing 101, wherein the top end of the push rod 301 extends out of the top of the housing 101, and the bottom end extends into the hollow shaft 105 and contacts with the top of the drill pin inner core 402 which also extends into the hollow shaft; as shown in fig. 1 and 7, the front side of the housing is provided with a sliding groove 102, a sliding block 701 capable of sliding along the sliding groove is provided on the inner side of the sliding groove 102, a locking screw 103 for locking the sliding block is further provided on the sliding groove, a locking seat 310 (shown in fig. 3) matched with the sliding block is provided on the push rod 301, and the upper limit movement position of the push rod 301 is limited by the cooperation of the sliding block 701 and the locking seat 310.
As shown in fig. 7, the slider 701 is further provided with a through hole 702, the push rod 301 passes through the through hole 702, and the slider 701 can slide freely along the push rod, so that the push rod can avoid the slider 701 from turning over.
As shown in fig. 3, the driving mechanism includes a driving motor 305 and a driving shaft 306 connected thereto, and the driving shaft 306 and the hollow shaft 105 are driven by a gear transmission or a belt transmission, preferably a gear transmission. Specifically, the hollow shaft 105 and the driving shaft 306 are supported and fixed by corresponding bearings respectively, each bearing is arranged on a corresponding fixed seat 312, a driving gear 308 is fixed on the driving shaft, a driven gear 309 is arranged on the hollow shaft 105, and the driving gear 308 and the driven gear 309 are meshed with each other to realize the gear transmission.
The shell is internally provided with a control circuit board and a battery 311, the battery 311 and the driving motor 305 are respectively connected with the control circuit board, and the front side surface of the shell is also provided with a key 104 and an indicator light 106 (shown in figure 1) which are connected with the control circuit board and used for controlling the start and stop of the driving motor and the rotating speed thereof. The battery 311 is preferably a rechargeable battery equipped with an efficient charging and protection circuit, and the housing is provided with a charging interface 204 connected to the battery.
In addition, as shown in fig. 3 and 5, a needle-removing device 302 is further provided in the housing, the top end of the needle-removing device extends out of the top of the housing, the bottom end extends out of the bottom of the housing and is connected with the sampling probe, a mounting groove 201 (as shown in fig. 2) is provided on the rear side of the housing, a protruding plate 202 bent backward and extending into the mounting groove 201 is provided on the needle-removing device, a spring (not shown in the drawings) is further provided in the mounting groove 201, one end of the spring is in contact connection with the bottom end of the inside of the mounting groove 201, and the other end of the spring is in contact connection with the protruding plate 202. Preferably, a sealing cover plate for packaging the spring is further arranged on the mounting groove 201.
As shown in fig. 3 and 5, the needle-removing device 302 is disposed behind the push rod 301, the top end of the needle-removing device is provided with a pressing portion 304 bent to one side, the bottom end of the needle-removing device is provided with a clamping portion 313 for conveniently clamping the sampling drill needle 501, and the needle-removing device is further provided with a protruding strip 501 bent forward and capable of being matched with the locking seat 310.
The housing 101 is formed by combining an upper case and a lower case with each other.
As shown in fig. 2, the upper half of the housing is a relatively small hand-held part, and the back of the housing is provided with a hook 203 which is bent downwards to facilitate hand holding.
As shown in fig. 8, the locking seat 310 is provided with a through hole 801 for passing through the push rod 301, and is further provided with a locking hole 802 for providing a jackscrew, and the locking seat 310 is locked on the push rod 301 when the jackscrew is screwed.
As shown in fig. 3, the top end of the push rod 301 is provided with a pressing round table 303 which is convenient to press.
Before the sampling operation, the sampling drill 401 and the sub-packaging sample tube need to be frozen, so that the sample is ensured to be in a low-temperature environment in all links of the operation.
The steps for sampling by the invention are as follows:
(1) And (5) switching on a power supply: the shell is internally integrated with a high-capacity rechargeable battery and is provided with a high-efficiency charging and protecting circuit, so that the battery can work with power, and the battery can continuously work for 1 hour after being disconnected from the power.
(2) Adjusting the sampling amount: the locking screw 103 is loosened and slid up and down to adjust the position of the slider 701, which serves to limit the upper limit movement position of the locking socket 310 (i.e., limit the sampling volume). The sampling volume is calculated by the formula v=sh (S is the bottom area and h is the height), where the inner diameter of the sampling drill needle 401 is a fixed value D, i.e. s=pi (D/2)/(2), and the height of the top end of the drill core in each sampling drill needle is uniform, because the bottom end of the push rod is initially in contact with the top end of the drill core, the position of the locking seat 310 on the push rod 301 is already set before the product is prepared and delivered, and a scale is formed on the housing, preferably near the slide groove, and the slide block 701 is fixed according to the scale, which defines the maximum travel of the locking seat from the initial position to the slide block, i.e. the sampling height h, so that the drilling volume is v=pi (D/2)/(2 h), thereby realizing the control of the sampling volume.
(3) Holding the handle, pricking the drill needle, and extending the inner core of the drill needle into the hollow shaft.
(4) Starting a split charging instrument: pressing the key 104, the driving motor 305 is started, and 1 is performed by two gears with the same size: 1, and then drives the hollow shaft 105 and drives the sampling drill needle 401 to rotate, at the moment, the driving motor is in a high speed gear with the speed of 17500RPM, and the sampling drill is used for drilling tissues with low hardness, such as blood and tissue fluid samples. Pressing the key 104 again, the driving motor is in a medium speed at 15500RPM for drilling tissue with a certain toughness, such as organ tissue samples and the like. Pressing the key 104 again, the drive motor is in low gear at 13500RPM for drilling a higher hardness sample. Pressing the key 104 again, the drive motor stops working.
(5) Drilling a sample: in the sample drilling process, the drilled tissue sample fills the space in the sampling drill needle, meanwhile, the drill needle inner core in the drill needle is pushed to ascend, the inner core linkage push rod 301 moves upwards, when the locking seat ascends to the sliding block position, the drill needle inner core also ascends to the maximum height, and at the moment, the sample cannot enter the drill needle, so that the drilling volume is controlled.
(6) Taking out a sample: pressing the key 104 stops the driving motor, pushes the push rod 301, and the push rod pushes the drill needle inner core, so that the drill sample is pushed out into the freezing storage tube. When the sample is pushed out completely, the pushing rod is pushed continuously, the pressing round table 303 at the top end of the pushing rod is contacted with the pressing part 304 at the top end of the needle abandoning device, and meanwhile the locking seat 310 on the pushing rod is contacted with the protruding strip 501 on the needle abandoning device, so that the needle abandoning device is pushed to beat off the sampling drill needle smoothly. After the hand is loosened, the spring in the mounting groove 201 can push the needle abandoning device to return to the initial position, so that the sample taking and needle abandoning operations are completed.
The foregoing description of the preferred embodiments of the present invention has been presented only to facilitate the understanding of the principles of the invention and its core concepts, and is not intended to limit the scope of the invention in any way, however, any modifications, equivalents, etc. which fall within the spirit and principles of the invention should be construed as being included in the scope of the invention.
Claims (9)
1. The utility model provides a handheld low temperature sample partial shipment appearance of adjustable speed, includes shell and sample drill point, its characterized in that: the sampling drill needle is connected with the hollow shaft in an interference fit manner, and a drill needle inner core extending out of the top end of the sampling drill needle is also arranged in the sampling drill needle; a push rod is further arranged in the shell, the top end of the push rod extends out of the top of the shell, and the bottom end of the push rod extends into the hollow shaft and contacts with the top of the drill needle inner core which also extends into the hollow shaft; the front side of the shell is provided with a sliding groove, the inner side of the sliding groove is provided with a sliding block capable of sliding along the sliding groove, the sliding block is provided with a through hole, the push rod passes through the through hole and can freely slide along the push rod, the sliding groove is also provided with a locking screw for locking the position of the sliding block, the push rod is provided with a locking seat matched with the sliding block, and the upper limit movement position of the push rod is limited by the matching of the sliding block and the locking seat;
the needle-removing device is arranged in the shell, the top end of the needle-removing device extends out of the top of the shell, the bottom end of the needle-removing device extends out of the bottom of the shell and is connected with the sampling drill needle, a mounting groove is formed in the rear side face of the shell, a protruding plate which is bent backwards and extends into the mounting groove is arranged on the needle-removing device, a spring is further arranged in the mounting groove, one end of the spring is in contact connection with the inner bottom end of the mounting groove, and the other end of the spring is in contact connection with the protruding plate.
2. The adjustable speed handheld cryogenic sample sub-packaging machine of claim 1, wherein: the driving mechanism comprises a driving motor and a driving shaft connected with the driving motor, and the driving shaft and the hollow shaft are driven in a gear transmission or belt transmission mode.
3. The adjustable speed hand-held cryogenic sample sub-packaging machine of claim 2, further comprising: the shell is internally provided with a control circuit board and a battery, the battery and the driving motor are respectively connected with the control circuit board, and the front side surface of the shell is also provided with a key which is connected with the control circuit board and used for controlling the start and stop of the driving motor and the rotating speed of the driving motor.
4. A speed adjustable hand held cryogenic sample sub-packaging machine according to claim 3, characterized in that: the battery is a rechargeable battery, and a charging interface connected with the battery is arranged on the shell.
5. The adjustable speed handheld cryogenic sample sub-packaging machine of claim 1, wherein: the needle abandoning device is arranged at the rear of the push rod, the top end of the needle abandoning device is provided with a pressing part bent to one side, and the needle abandoning device is also provided with a protruding strip bent forward and matched with the locking seat.
6. The adjustable speed hand-held cryogenic sample sub-packaging machine according to any one of claims 1-5, wherein: the shell is formed by combining an upper shell and a lower shell.
7. The adjustable speed hand-held cryogenic sample sub-packaging machine according to any one of claims 1-5, wherein: the upper half part of the shell is a hand-held part, and the back of the shell is provided with a hook body which is convenient to hold and bends downwards.
8. The adjustable speed hand-held cryogenic sample sub-packaging machine according to any one of claims 1-5, wherein: the locking seat is locked on the ejector rod through the jackscrew, the hollow shaft is connected with a bearing, and the bearing is arranged on a fixed seat in the shell.
9. The adjustable speed hand-held cryogenic sample sub-packaging machine according to any one of claims 1-5, wherein: the top of push rod is equipped with the round platform of pressing of being convenient for press.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711395849.3A CN107843453B (en) | 2017-12-21 | 2017-12-21 | Speed-adjustable handheld low-temperature sample split charging instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711395849.3A CN107843453B (en) | 2017-12-21 | 2017-12-21 | Speed-adjustable handheld low-temperature sample split charging instrument |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107843453A CN107843453A (en) | 2018-03-27 |
| CN107843453B true CN107843453B (en) | 2024-01-23 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711395849.3A Active CN107843453B (en) | 2017-12-21 | 2017-12-21 | Speed-adjustable handheld low-temperature sample split charging instrument |
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| Country | Link |
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| US9134202B2 (en) * | 2012-01-26 | 2015-09-15 | Cryoxtract Instruments, Llc | Robotic end effector for frozen aliquotter and methods of taking a frozen aliquot from biological samples |
| WO2014018681A2 (en) * | 2012-07-24 | 2014-01-30 | Cryoxtract Instruments, Llc | Apparatus and methods for aliquotting frozen samples |
| CN105899931A (en) * | 2013-05-04 | 2016-08-24 | 克莱奥克斯特拉克特仪器有限责任公司 | Targeting systems and methods for frozen aliquotter for biological samples |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS54118164A (en) * | 1978-03-07 | 1979-09-13 | Hitachi Ltd | Frozen sample manufacturing carrier for scanning electronic microscope |
| US4706207A (en) * | 1985-06-24 | 1987-11-10 | Nova Celltrak, Inc. | Count accuracy control means for a blood analyses system |
| JPH01187524A (en) * | 1988-01-22 | 1989-07-26 | Hitachi Ltd | Method and device for taking frozen sample |
| JPH10142123A (en) * | 1996-11-08 | 1998-05-29 | Hitachi Ltd | Rapid freezing apparatus |
| DE10212139A1 (en) * | 2002-03-19 | 2003-10-09 | Norbert F Heske | Biopsy device consists of a handpiece, a needle, a vacuum generating unit located in the handpiece housing, a ventilation opening, and a connecting element |
| CA2380736A1 (en) * | 2002-04-02 | 2003-10-02 | Joel S. Harris | Motor driven sampling apparatus for material collection |
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Also Published As
| Publication number | Publication date |
|---|---|
| CN107843453A (en) | 2018-03-27 |
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