CN107372470A - A kind of ovum embryo vitrifying freeze save set - Google Patents
A kind of ovum embryo vitrifying freeze save set Download PDFInfo
- Publication number
- CN107372470A CN107372470A CN201710813869.1A CN201710813869A CN107372470A CN 107372470 A CN107372470 A CN 107372470A CN 201710813869 A CN201710813869 A CN 201710813869A CN 107372470 A CN107372470 A CN 107372470A
- Authority
- CN
- China
- Prior art keywords
- embryo
- push rod
- ovum
- inner sleeve
- save set
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 75
- 210000004681 ovum Anatomy 0.000 title claims abstract description 33
- 102000002322 Egg Proteins Human genes 0.000 title claims abstract description 30
- 108010000912 Egg Proteins Proteins 0.000 title claims abstract description 30
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 18
- 230000000712 assembly Effects 0.000 claims abstract description 16
- 238000000429 assembly Methods 0.000 claims abstract description 16
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 238000001914 filtration Methods 0.000 claims abstract description 5
- 238000000605 extraction Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 abstract description 7
- 238000010276 construction Methods 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 230000001681 protective effect Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000005057 refrigeration Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 210000001672 ovary Anatomy 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000004017 vitrification Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0236—Mechanical aspects
- A01N1/0242—Apparatuses, i.e. devices used in the process of preservation of living parts, such as pumps, refrigeration devices or any other devices featuring moving parts and/or temperature controlling components
- A01N1/0252—Temperature controlling refrigerating apparatus, i.e. devices used to actively control the temperature of a designated internal volume, e.g. refrigerators, freeze-drying apparatus or liquid nitrogen baths
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Thermal Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Dentistry (AREA)
- Health & Medical Sciences (AREA)
- Mechanical Engineering (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of ovum embryo vitrifying freeze save set, the present invention not only can there is provided special embryo to insert component, so, embryo is when inserting, what can be changed protects to ovum embryo, prevent the influence to ovum embryo during inserting, simultaneously, the present invention sets inner sleeve in vacuo, the device is arranged in liquid nitrogen, preservation effect and quality can be improved, the present invention in inner sleeve set in filter assemblies, and the multiple filter membranes for being arranged to stack by interior filter assemblies, filtration can be ensured, improve the quality of nitrogen in inner sleeve, the present invention is simple in construction, it is easy to use, preserved while multiple ovum embryos can be realized, and improve the convenience of preservation.
Description
Technical field
The present invention relates to a kind of ovum embryo vitrifying freeze save set, and it is auxiliary to belong to the preservation of ovum embryo vitrifying freeze
Help equipment technical field.
Background technology
At present, the general principle of vitrifying freeze process is to solidify the cryoprotector of high concentration under ultra-low temperature surroundings,
Irregular vitrifying sample solid is formed, normal molecular and ion distribution when saving liquid, thus glass occurs in the cell
It can be played a protective role during change.After preserved cell is dehydrated to a certain extent in frozen solution, then cause endogenous kytoplasm
Macromolecular such as protein and the cryoprotector concentration for penetrating into intracellular, so that cell is protected in violent fast cooling
Shield.The gamete of the mankind and the freezen protective of embryo have been a necessary parts in artificial field of reproduction, the past this 20
Nian Zhong, the development of freezen protective technology can also reflect the development degree of Artificial reproduction technology, and present Refrigeration Technique can be used in
The embryo of freezen protective sperm, ovum and different times, especially vitrification, it is extensive that it has not only obtained medical field
Accreditation, and the slow art freezing of substitution tradition gradually, and as the optimal selection of Refrigeration Technique, even by this Technology application in height
In the ovum of difficulty, ovary tissue and the freezing of hESC.
And vitrifying freeze process needs to realize to embryo's using special ovum embryo vitrifying freeze save set
Preserve, still, current save set is relatively simple for structure, is usually preserved and is arranged in liquid nitrogen, still, this structure
Save set it is fairly simple, when inserting ovum embryo, it is difficult to be accurately and rapidly put into, during being put into, easily by outer
Boundary is influenceed, and harmful effect is produced to embryo.
The present invention is directed to problem above, there is provided a kind of ovum embryo vitrifying freeze save set, to improve ovum embryo
The quality of tire freezen protective.
The content of the invention
To achieve the above object, the present invention provides following technical scheme：A kind of ovum embryo vitrifying freeze save set,
It includes outer cover body, interior bearing, inner sleeve, interior filter assemblies and embryo and inserts component, it is characterised in that the outer cover body
Inside is fixedly installed the inner sleeve using interior seat supports, and the cavity between the inner sleeve and the outer cover body is to take out
Vacuum is set, and the both sides of the inner sleeve are respectively arranged with a set of interior filter assemblies, and the both ends of the inner sleeve are connected with
Nitrogen pipe is noted, the note nitrogen pipe stretches out the outer cover body and set, set in the inner sleeve between two sets of filter assemblies
At least one embryo's positioning seat is equipped with, embryo is provided with embryo's positioning seat and positions set-point, the side of the outer cover body
Be provided with wall and stretch into positioning hole, be provided with inner sleeve with the connecting hole for stretching into positioning hole and connecting on outer cover body, it is described
Embryo insert component can stretch into extraction be positioned at it is described stretch into positioning hole and connecting hole, the positioning hole and connecting hole
Inside it is additionally provided with dismountable freeze-resistant sealing-plug.
Further, preferably, the inner sleeve includes intervalve and capped pipe, the both ends of the intervalve are threadedly coupled
The capped pipe is provided with, the end of the capped pipe is connected with note nitrogen pipe, and the positioning hole that stretches into is arranged in the side wall of intervalve,
The outer end of the flexible positioning hole is provided with the detent of step type.
Further, preferably, the interior filter assemblies include connection round bar, fixed disk, filter membrane and filter membrane stator, its
In, it is more through the centrally disposed of the fixed disk that the fixed disk is arranged on the connection round bar on the inwall of the intervalve
The individual filter membrane is connected on round bar through the connection round bar to be fixed on.
Further, preferably, the external diameter of the filter membrane in the side away from embryo's positioning seat, which is more than, is in close embryo
The filter membrane of the side of positioning seat.
Further, preferably, the embryo, which inserts component, includes push rod, spring, push rod overcoat, elastic parts and sealing
Part, wherein, the end set of the push rod has embryo's positioning head, and embryo is placed in embryo's positioning head when being put into the device
On, the push rod is co-axially located in the push rod overcoat, and seal is additionally provided with the push rod overcoat, and the push rod passes through
The seal is set, and the elastic parts, embryo's positioning head are additionally provided between the push rod and the push rod overcoat
The end for stretching out in the push rod overcoat can be stretched into, and the end set of the push rod overcoat has and stretched into positioning hole and connection
The ladder shaft end that hole coordinates.
Further, preferably, the elastic parts includes fixed mount, fixed ear and spring, wherein, the fixed mount is consolidated
Surely it is set on the push rod, the fixed ear is fixed on the push rod outer casing inner wall, the fixed ear and the fixed mount
Between be provided with spring.
Further, preferably, the device in liquid nitrogen container as being preserved.
Further, preferably, the outside of the push rod is provided with push handle.
Further, preferably, on the push rod positioned at multi-diameter shaft end side be provided with to the push rod carry out it is spacing
Limited shoulder.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention not only can there is provided special embryo to insert component, so, embryo when inserting, can change to ovum
Embryo is protected, and prevents the influence to ovum embryo during inserting, meanwhile, the present invention sets inner sleeve in vacuo,
The device is arranged in liquid nitrogen, can improve preservation effect and quality, the present invention set in inner sleeve in filter assemblies, and
Multiple filter membranes that interior filter assemblies are arranged to stack, it is ensured that filtration, the quality of nitrogen in inner sleeve is improved,
The present invention is simple in construction, easy to use, it is possible to achieve to be preserved while multiple ovum embryos, and improve the convenience of preservation.
Brief description of the drawings
Fig. 1 is a kind of structural representation of ovum embryo vitrifying freeze save set of the present invention；
Fig. 2 is the structural representation that a kind of embryo of ovum embryo vitrifying freeze save set of the present invention inserts component.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Fig. 1-2 is referred to, the present invention provides a kind of technical scheme：A kind of ovum embryo vitrifying freeze save set, its
Component is inserted including outer cover body 1, interior bearing 2, inner sleeve, interior filter assemblies and embryo, it is characterised in that the outer cover body
1 inside is fixedly installed the inner sleeve using the support of interior bearing 2, and the cavity between the inner sleeve and the outer cover body is
Setting is vacuumized, the both sides of the inner sleeve are respectively arranged with a set of interior filter assemblies, the both ends connection of the inner sleeve
Have note nitrogen pipe 9, the note nitrogen pipe 9 stretches out the outer cover body and set, in the inner sleeve positioned at two sets of filter assemblies it
Between be provided with least one embryo's positioning seat 10, be provided with embryo's positioning seat 10 embryo position set-point, the shell
It is provided with the side wall of body 1 and stretches into positioning hole, is provided with inner sleeve and the company for stretching into positioning hole and connecting on outer cover body
Connect hole, the embryo insert component can stretch into extraction be positioned at it is described stretch into positioning hole and connecting hole, the positioning hole
And dismountable freeze-resistant sealing-plug is additionally provided with connecting hole.
Wherein, the inner sleeve includes intervalve 4 and capped pipe 3, and the both ends of the intervalve 4 have been threadedly coupled setting
Capped pipe 3 is stated, the end of the capped pipe 3 is connected with note nitrogen pipe 9, and the positioning hole that stretches into is arranged in the side wall of intervalve, described
The outer end of flexible positioning hole is provided with the detent of step type.
The interior filter assemblies include connection round bar 6, fixed disk 5, filter membrane 7 and filter membrane stator 8, wherein, the fixation
Disk 5 is arranged on centrally disposed, multiple filter membranes 7 that the connection round bar on the inwall of the intervalve passes through the fixed disk
Connected through the connection round bar to be fixed on round bar 6.Wherein, outside the filter membrane of the side in remote embryo's positioning seat
Footpath is more than the filter membrane being in close to the side of embryo's positioning seat.
In the present embodiment, the embryo insert component include push rod 12, spring 14, push rod overcoat 13, elastic parts and
Seal 19, wherein, the end set of the push rod 12 has embryo's positioning head 20, and embryo 11 is placed in institute when being put into the device
State on embryo's positioning head 20, the push rod 12 is co-axially located in the push rod overcoat 13, is also set up in the push rod overcoat 13
There is seal 19, the push rod 12 sets through the seal 19, also set between the push rod 12 and the push rod overcoat 13
The elastic parts is equipped with, embryo's positioning head can stretch into the end for stretching out in the push rod overcoat, and the push rod overcoat
End set have and stretch into the ladder shaft end that positioning hole and connecting hole coordinate.
Such as Fig. 2, the elastic parts includes fixed mount 17, fixed ear 18 and spring 14, wherein, the fixed mount 17 is fixed
Be set on the push rod 12, the fixed ear 18 is fixed on the push rod outer casing inner wall, the fixed ear 18 with it is described solid
Determine to be provided with spring 14 between frame 17.
Wherein, the device in liquid nitrogen container as being preserved.The outside of the push rod is provided with push handle 15.The push rod 12
The upper side positioned at multi-diameter shaft end, which is provided with, carries out spacing limited shoulder to the push rod.
The present invention not only can there is provided special embryo to insert component, so, embryo is when inserting, pair that can change
Ovum embryo is protected, and prevents the influence to ovum embryo during inserting, meanwhile, inner sleeve is arranged on vacuum by the present invention
It is interior, the device is arranged in liquid nitrogen, can improve preservation effect and quality, the present invention set in inner sleeve in filtering group
Part, and multiple filter membranes that interior filter assemblies are arranged to stack, it is ensured that filtration, improve nitrogen in inner sleeve
Quality, the present invention is simple in construction, easy to use, it is possible to achieve to be preserved while multiple ovum embryos, and improve the convenience of preservation
Property.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (9)
1. a kind of ovum embryo vitrifying freeze save set, it includes outer cover body, interior bearing, inner sleeve, interior filter assemblies
Component is inserted with embryo, it is characterised in that the inner sleeve is fixedly installed using interior seat supports inside the outer cover body,
To vacuumize setting, the both sides of the inner sleeve are respectively arranged with a set of for cavity between the inner sleeve and the outer cover body
The interior filter assemblies, the both ends of the inner sleeve are connected with note nitrogen pipe, and the note nitrogen pipe stretches out the outer cover body and set, institute
State in inner sleeve and be provided with least one embryo's positioning seat between two sets of filter assemblies, set on embryo's positioning seat
It is equipped with embryo and positions set-point, be provided with the side wall of the outer cover body and stretch into positioning hole, is provided with inner sleeve and shell
The connecting hole for stretching into positioning hole connection on body, the embryo, which inserts component and can stretch into being positioned at for extraction, described stretches into positioning
In hole and connecting hole, dismountable freeze-resistant sealing-plug is additionally provided with the positioning hole and connecting hole.
A kind of 2. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：The inner sleeve
Including intervalve and capped pipe, the both ends of the intervalve, which are threadedly coupled, is provided with the capped pipe, the end of the capped pipe and note
Nitrogen pipe is connected, and the positioning hole that stretches into is arranged in the side wall of intervalve, and the outer end of the flexible positioning hole is provided with step type
Detent.
A kind of 3. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：The interior filtering
Component includes connection round bar, fixed disk, filter membrane and filter membrane stator, wherein, the fixed disk is arranged on the interior of the intervalve
The connection round bar connects round bar to fix through the centrally disposed of the fixed disk, multiple filter membranes through described on wall
On connection round bar.
A kind of 4. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：In away from embryo
The external diameter of the filter membrane of the side of tire positioning seat is more than the filter membrane being in close to the side of embryo's positioning seat.
A kind of 5. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：The embryo puts
Entering component includes push rod, spring, push rod overcoat, elastic parts and seal, wherein, the end set of the push rod has embryo to determine
Potential head, embryo are placed in when being put into the device on embryo's positioning head, and the push rod is co-axially located at the push rod overcoat
It is interior, seal is additionally provided with the push rod overcoat, the push rod is set through the seal, the push rod and the push rod
The elastic parts is additionally provided between overcoat, embryo's positioning head can stretch into the end for stretching out in the push rod overcoat, and
The end set of the push rod overcoat has and stretched into the ladder shaft end that positioning hole and connecting hole coordinate.
A kind of 6. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：Described elastic group
Part includes fixed mount, fixed ear and spring, wherein, the fixed mount is fixed and is set on the push rod, and the fixed ear is fixed
On the push rod outer casing inner wall, spring is provided between the fixed ear and the fixed mount.
A kind of 7. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：The device as
Preserved in liquid nitrogen container.
A kind of 8. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：The push rod
Outside is provided with push handle.
A kind of 9. ovum embryo vitrifying freeze save set according to claim 1, it is characterised in that：On the push rod
It is provided with positioned at the side of ladder shaft end and spacing limited shoulder is carried out to the push rod.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710813869.1A CN107372470B (en) | 2017-09-11 | 2017-09-11 | Ovum embryo vitrification cryopreservation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710813869.1A CN107372470B (en) | 2017-09-11 | 2017-09-11 | Ovum embryo vitrification cryopreservation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107372470A true CN107372470A (en) | 2017-11-24 |
| CN107372470B CN107372470B (en) | 2021-05-28 |
Family
ID=60351094
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710813869.1A Expired - Fee Related CN107372470B (en) | 2017-09-11 | 2017-09-11 | Ovum embryo vitrification cryopreservation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107372470B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109997841A (en) * | 2019-04-09 | 2019-07-12 | 上海交通大学 | Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere |
| CN110024776A (en) * | 2019-05-16 | 2019-07-19 | 刘文静 | A kind of dedicated embryo's storage device of vitrifying freeze thawing |
| CN110057821A (en) * | 2019-04-16 | 2019-07-26 | 上海交通大学 | Cryo-microscope imaging system for mankind's gamete fast freeze-thaw process observation |
| WO2020147604A1 (en) * | 2019-01-18 | 2020-07-23 | 上海廪典实业有限公司 | Ultralow-temperature freezing and sealing type preservation method |
| CN114467923A (en) * | 2022-02-16 | 2022-05-13 | 上海少卿白鞘医疗科技有限公司 | External electromagnetic type contact temperature control gamete freezing cold source device |
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| CN101473179A (en) * | 2006-07-13 | 2009-07-01 | B·西克斯特 | Transport container for maintaining the temperature of frozen goods |
| CN105163704A (en) * | 2013-02-22 | 2015-12-16 | 莱福奈特海尔斯公司 | Packaging assembly for storing tissue and cellular material |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020147604A1 (en) * | 2019-01-18 | 2020-07-23 | 上海廪典实业有限公司 | Ultralow-temperature freezing and sealing type preservation method |
| CN109997841A (en) * | 2019-04-09 | 2019-07-12 | 上海交通大学 | Maintain mankind's gamete fast freeze-thaw visible environment cabin of atmospheric air atmosphere |
| CN109997841B (en) * | 2019-04-09 | 2021-02-02 | 上海交通大学 | Human gamete rapid freezing and thawing visualization environment cabin maintaining atmospheric air atmosphere |
| CN110057821A (en) * | 2019-04-16 | 2019-07-26 | 上海交通大学 | Cryo-microscope imaging system for mankind's gamete fast freeze-thaw process observation |
| CN110024776A (en) * | 2019-05-16 | 2019-07-19 | 刘文静 | A kind of dedicated embryo's storage device of vitrifying freeze thawing |
| CN114467923A (en) * | 2022-02-16 | 2022-05-13 | 上海少卿白鞘医疗科技有限公司 | External electromagnetic type contact temperature control gamete freezing cold source device |
| CN114467923B (en) * | 2022-02-16 | 2024-02-02 | 上海少卿白鞘医疗科技有限公司 | External electromagnetic contact control Wen Peizi refrigeration cold source device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107372470B (en) | 2021-05-28 |
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