CN204474668U - The Human umbilical vein endothelial cells tripping device improved - Google Patents
The Human umbilical vein endothelial cells tripping device improved Download PDFInfo
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- CN204474668U CN204474668U CN201520108343.XU CN201520108343U CN204474668U CN 204474668 U CN204474668 U CN 204474668U CN 201520108343 U CN201520108343 U CN 201520108343U CN 204474668 U CN204474668 U CN 204474668U
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- Prior art keywords
- plexi
- glass tubular
- endothelial cells
- umbilical vein
- vein endothelial
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- 210000003606 umbilical vein Anatomy 0.000 title claims abstract description 36
- 210000002889 endothelial cell Anatomy 0.000 title claims abstract description 30
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims abstract description 48
- 230000006872 improvement Effects 0.000 claims abstract description 8
- 210000003954 umbilical cord Anatomy 0.000 abstract description 36
- 239000002904 solvent Substances 0.000 description 14
- 239000002953 phosphate buffered saline Substances 0.000 description 11
- 210000004027 cell Anatomy 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 210000003462 vein Anatomy 0.000 description 6
- 238000002347 injection Methods 0.000 description 5
- 239000007924 injection Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 4
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000013016 damping Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 210000003725 endotheliocyte Anatomy 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000700721 Hepatitis B virus Species 0.000 description 1
- 244000141353 Prunus domestica Species 0.000 description 1
- 208000035010 Term birth Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
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- Materials For Medical Uses (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The utility model provides a kind of Human umbilical vein endothelial cells tripping device of improvement, comprising: plexi-glass tubular, and its two ends are opening, and the tube wall two ends of plexi-glass tubular are respectively equipped with a perforate; Two rubber plugs, it is located at the opening at plexi-glass tubular two ends respectively; Two communicating vesselss, it wears the rubber plug at plexi-glass tubular both ends open place respectively.The utility model can be used in extracting Human umbilical vein endothelial cells, and Cell protection is not contaminated, avoids umbilical cord to the pollution of environment simultaneously, and its structure is simple, operational safety.
Description
Technical field
The utility model relates to biomedical sector, refers to a kind of tripping device, refers to a kind of Human umbilical vein endothelial cells tripping device of improvement especially.
Background technology
Human umbilical vein endothelial cells (Human umbilical vein endothelial cells, be called for short HUVEC), be separated in the Human Umbilical Cord Vein blood vessel by term birth to get and can the cell of vitro culture, due to its to the reactivity of medicine or stimulation closest to human body and relatively easily obtain, become the important tool of medical basic research for many years.
Be separated and obtaining in the process of Human umbilical vein endothelial cells at present, because the difference of the device, method and the gimmick that use causes the quality of cell uneven, very large impact is being caused on the stability of experimental result.Existing isolation technique is placed on by umbilical cord to fill phosphate buffered saline buffer (Phosphate Buffered Saline, be called for short PBS) open vessel in operate, and be separated the device i.e. simple open vessel that use, such device is to the sterility requirements of surrounding environment height very, be very easy to cause the pollution to cell, and be very easy to cause the pollution to surrounding environment.
Because above-mentioned prior art Problems existing, the present inventor, in conjunction with association area design for many years and use experience, was aided with strong expertise, provided a kind of Human umbilical vein endothelial cells tripping device of improvement to overcome above-mentioned defect.
Utility model content
The purpose of this utility model is to provide a kind of Human umbilical vein endothelial cells tripping device of improvement, and it can be used in extracting Human umbilical vein endothelial cells, and Cell protection is not contaminated, avoids umbilical cord to the pollution of environment simultaneously, and its structure is simple, operational safety.
Above-mentioned purpose of the present utility model can adopt following technical proposal to realize:
A Human umbilical vein endothelial cells tripping device for improvement, it comprises: plexi-glass tubular, and its two ends are opening, and the tube wall two ends of described plexi-glass tubular are respectively equipped with a perforate; Two rubber plugs, it is located at the opening at described plexi-glass tubular two ends respectively; Two communicating vesselss, it wears the rubber plug at described plexi-glass tubular both ends open place respectively.
In a preferred embodiment, described perforate is connected with flexible pipe, and described flexible pipe is folded with switch.
In a preferred embodiment, the retractable center being arranged in described rubber plug of described communicating vessels.
In a preferred embodiment, one end that described communicating vessels is positioned at plexi-glass tubular inside is provided with fixed part, and the external diameter of described fixed part is greater than the external diameter of described communicating vessels.
In a preferred embodiment, the length of described plexi-glass tubular is 30cm.
In a preferred embodiment, the internal diameter of described plexi-glass tubular is 2.5cm.
The feature of the Human umbilical vein endothelial cells tripping device that the utility model improves and advantage are: closed by the opening of rubber plug to plexi-glass tubular two ends, make plexi-glass tubular inside keep sealed environment; Communicating vessels is flexible in rubber plug to be worn, not only can the distance between the communicating vessels at plexi-glass tubular two ends be regulated, and then make it be applicable to the umbilical cord of different lengths, but also can prevent the endovascular solvent of injection umbilical vein from leaking out in plexi-glass tubular while injecting solvent to umbilical vein Ink vessel transfusing; The fixed part that one end that communicating vessels enters organic glass tube through rubber plug is provided with, can be used for effectively fixing umbilical cord vein blood vessel; Be connected with the perforate on plexi-glass tubular by flexible pipe, PBS solvent can be poured into plexi-glass tubular inside, moistening to maintain umbilical cord.The utility model for extracting Human umbilical vein endothelial cells, both can Cell protection not contaminated, umbilical cord also can be avoided the pollution of environment, its structure is simple, operational safety.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme in the utility model embodiment, below the accompanying drawing used required in describing embodiment is briefly described, apparently, accompanying drawing in the following describes is only embodiments more of the present utility model, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the schematic front view of the Human umbilical vein endothelial cells tripping device that the utility model improves.
Fig. 2 is the schematic perspective view of the Human umbilical vein endothelial cells tripping device that the utility model improves.
Drawing reference numeral illustrates:
1 plexi-glass tubular, 2 openings, 3 perforates, 4 flexible pipes, 5 communicating vesselss, 6 rubber plugs, 7 fixed parts, 8 connector portions.
Embodiment
In order to have clearer understanding to the technical solution of the utility model, object and effect, existing accompanying drawings embodiment of the present utility model.
As depicted in figs. 1 and 2, the utility model provides a kind of Human umbilical vein endothelial cells tripping device of improvement, and belong to a kind of experimental installation of biological species, it comprises: plexi-glass tubular 1, its two ends are opening 2, and the tube wall two ends of described plexi-glass tubular 1 are respectively equipped with a perforate 3; Two rubber plugs 6, it is located at the opening 2 at described plexi-glass tubular 1 two ends respectively; Two communicating vesselss 5, it wears the rubber plug 6 at described plexi-glass tubular 1 both ends open 2 place respectively.
Further, the retractable center being arranged in rubber plug 6 of communicating vessels 5, make the distance between two communicating vesselss 5 at plexi-glass tubular 1 two ends adjustable, one end that communicating vessels 5 is positioned at plexi-glass tubular 1 inside is provided with fixed part 7, the external diameter of fixed part 7 is greater than the external diameter of communicating vessels 5, preferably, fixed part 7 expand shape rounded relative to communicating vessels 5, one end that communicating vessels 5 exposes plexi-glass tubular 1 outside is provided with connector portions 8, and communicating vessels 5, fixed part 7 and connector portions 8 are inside middle conducting, the connector portions 8 of communicating vessels 5 can connect syringe, syringe is made to inject solvent by connector portions 8 and communicating vessels 5 to umbilical vein Ink vessel transfusing, in the present embodiment, this solvent can be PBS damping fluid or collagenase, the object injecting PBS damping fluid in umbilical cord is the remained blood of rinsing umbilical vein, in umbilical cord, the object of By Injection of Collagenase is digestion endotheliocyte, then collects.In umbilical cord, injection and/or recovered solvent are all undertaken by connector portions 8.Meanwhile, the communicating vessels 5 in the utility model also can select animalcule gastric perfusion needle to replace.
Further, perforate 3 is connected with flexible pipe 4, flexible pipe 4 is folded with switch (not shown in FIG.), can inject solvent by flexible pipe 4 and perforate 3 to plexi-glass tubular 1 inside.
General umbilical cord length is at about 40cm, about 30cm is approximately after inappropriate part that prunes away, and the requirement of experiment umbilical cord length extracting cell can not be less than 20cm, therefore in a preferred embodiment, plexi-glass tubular 1 is the hollow cylinder of even thickness, and the length of plexi-glass tubular 1 is 30cm, the internal diameter of plexi-glass tubular 1 is 2.5cm, namely can be met the requirement of experiment of umbilical cord length by the telescopic adjustment of communicating vessels 5 in rubber plug 6, make this plant bulk size be applicable to the application of umbilical cord size.
The utility model before use, first by all article autoclavings, and carries out process in early stage to pending umbilical cord, cutting the damaged place with there being extravasated blood, then isolating the umbilical cord vein blood vessel at umbilical cord two ends, being fixed on fixed part 7; When fixing, first one end of umbilical cord is fixed on the fixed part 7 of a communicating vessels 5, then umbilical cord is made to pass plexi-glass tubular 1, the other end of umbilical cord is used the same method be fixed on plexi-glass tubular 1 the other end communicating vessels 5 fixed part 7 on, preferably, also by surgical thread, umbilical cord and fixed part 7 can be tied up, make it connect more firm; Then opening 2 place of rubber plug 6 fixed plug at plexi-glass tubular 1 two ends of communicating vessels 5 will be equipped with, and by the communicating vessels 5 at the two ends mobile length regulated between two ends communicating vessels 5 of shuttling back and forth in rubber plug 6, to keep umbilical cord without inflection, distortionless and moderate length.
The utility model is in cellular segregation operating process, first the Human umbilical vein endothelial cells tripping device of the improvement connected is arranged on anchor, it is made to remain on about 30 ° of angles tilted, now the opening of the flexible pipe 4 of upper end vertically rushes to just, and the opening of the flexible pipe 4 of lower end vertically sweeps away; Then in plexi-glass tubular 1, inject aseptic PBS solvent by the flexible pipe 4 of upper end, make plexi-glass tubular 1 inside keep liquid environment, and then make umbilical cord keep moistening, then close the switch on flexible pipe 4, make umbilical cord be in aseptic opposing seal environment; PBS solvent is injected again from the communicating vessels 5 of upper end to umbilical vein Ink vessel transfusing, umbilical cord vein blood vessel is rinsed, flushed PBS solvent is discharged from the communicating vessels 5 of lower end, and then by the communicating vessels 5 of upper end to By Injection of Collagenase in umbilical cord vein blood vessel, and quietly put 15 minutes, to digest endotheliocyte, cellular segregation is got off, and the liquid extracted out by the communicating vessels 5 of lower end in umbilical vein blood vessel, namely the Human umbilical vein endothelial cells separated is contained in this liquid, finally with PBS solvent, umbilical cord vein blood vessel is rinsed, to obtain more cell.In whole operating process, without the need to contacting umbilical cord again, safety simple to operate, had both protected cell not contaminated, turn avoid the pollution of umbilical cord to environment and the infection to operator.
The Human umbilical vein endothelial cells tripping device that the utility model improves, is closed by the opening 2 at rubber plug 6 pairs of plexi-glass tubular 1 two ends, makes plexi-glass tubular 1 inside keep sealed environment; Communicating vessels 5 is flexible in rubber plug 6 to be worn, not only can the distance between the communicating vessels 5 at plexi-glass tubular 1 two ends be regulated, and then make it be applicable to the umbilical cord of different lengths, but also can prevent the endovascular solvent of injection umbilical vein from leaking out in plexi-glass tubular 1 while injecting solvent to umbilical vein internal blood vessel; The fixed part 7 that one end that communicating vessels 5 enters plexi-glass tubular 1 inside through rubber plug 6 is provided with, can be used for effectively fixing umbilical cord vein blood vessel; Be connected with the perforate 3 on plexi-glass tubular 1 by flexible pipe 4, PBS solvent can be poured into plexi-glass tubular 1 inside, moistening to maintain umbilical cord.The utility model is for extracting Human umbilical vein endothelial cells; both can Cell protection not contaminated; umbilical cord also can be avoided the pollution of environment comprising operator; such as umbilical cord is from hepatitis B virus carriers or the people having other diseases; in operation; can effectively umbilical cord and operator be isolated by the utility model, the infected probability of minimizing operator, its structure is simple, operational safety.
The foregoing is only the schematic embodiment of the utility model, and be not used to limit scope of the present utility model.Any those skilled in the art, the equivalent variations done under the prerequisite not departing from principle of the present utility model and design and amendment, all should belong to protection domain of the present utility model.
Claims (6)
1. the Human umbilical vein endothelial cells tripping device improved, it is characterized in that, the Human umbilical vein endothelial cells tripping device of described improvement comprises:
Plexi-glass tubular, its two ends are opening, and the tube wall two ends of described plexi-glass tubular are respectively equipped with a perforate;
Two rubber plugs, it is located at the opening at described plexi-glass tubular two ends respectively;
Two communicating vesselss, it wears the rubber plug at described plexi-glass tubular both ends open place respectively.
2. the Human umbilical vein endothelial cells tripping device improved according to claim 1, is characterized in that, described perforate is connected with flexible pipe, described flexible pipe is folded with switch.
3. the Human umbilical vein endothelial cells tripping device improved according to claim 1, is characterized in that, the retractable center being arranged in described rubber plug of described communicating vessels.
4. the Human umbilical vein endothelial cells tripping device improved according to claim 1, is characterized in that, one end that described communicating vessels is positioned at plexi-glass tubular inside is provided with fixed part, and the external diameter of described fixed part is greater than the external diameter of described communicating vessels.
5. the Human umbilical vein endothelial cells tripping device improved according to claim 1, is characterized in that, the length of described plexi-glass tubular is 30cm.
6. the Human umbilical vein endothelial cells tripping device improved according to claim 1, is characterized in that, the internal diameter of described plexi-glass tubular is 2.5cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520108343.XU CN204474668U (en) | 2015-02-13 | 2015-02-13 | The Human umbilical vein endothelial cells tripping device improved |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520108343.XU CN204474668U (en) | 2015-02-13 | 2015-02-13 | The Human umbilical vein endothelial cells tripping device improved |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204474668U true CN204474668U (en) | 2015-07-15 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520108343.XU Expired - Fee Related CN204474668U (en) | 2015-02-13 | 2015-02-13 | The Human umbilical vein endothelial cells tripping device improved |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN204474668U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058218A (en) * | 2017-06-16 | 2017-08-18 | 首都医科大学 | The separation of pregnant mouse placenta vascular smooth muscle cells and propagating method |
| CN109998601A (en) * | 2019-04-25 | 2019-07-12 | 河南卓丰生物科技有限公司 | A kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue |
-
2015
- 2015-02-13 CN CN201520108343.XU patent/CN204474668U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107058218A (en) * | 2017-06-16 | 2017-08-18 | 首都医科大学 | The separation of pregnant mouse placenta vascular smooth muscle cells and propagating method |
| CN109998601A (en) * | 2019-04-25 | 2019-07-12 | 河南卓丰生物科技有限公司 | A kind of up-stripping device for people's umbilical cord periphery China Tong Shi glue |
| CN109998601B (en) * | 2019-04-25 | 2021-07-20 | 河南卓丰生物科技有限公司 | A supplementary stripping off device that is used for human umbilical cord periphery huatong glue |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150715 Termination date: 20190213 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |