CN215913099U - Closed vitrification carrier - Google Patents
Closed vitrification carrier Download PDFInfo
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- CN215913099U CN215913099U CN202122361794.2U CN202122361794U CN215913099U CN 215913099 U CN215913099 U CN 215913099U CN 202122361794 U CN202122361794 U CN 202122361794U CN 215913099 U CN215913099 U CN 215913099U
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Abstract
The utility model relates to a closed vitrification freezing carrier, which is provided with a carrier; the carrier is formed by sleeving an outer sleeve, an inserting rod, a carrying rod and a slide glass; one end of the outer sleeve is open and the other end is closed, and metal is arranged on the inner side of the closed end; the inserted bar is solid, and the diameter of the inserted bar is reduced at three quarters of the length; the carrying rod is connected with the slide glass, the carrying rod is tubular, and the slide glass is flaky and provided with radian; the inserted bar can be sleeved in the carrying bar; the inserted bar, the carrying bar and the carrying sheet are sleeved in the outer sleeve; the outer sleeve is used for completely sleeving the inserted rod, the carrying rod and the carrying sheet inside. Its advantages are: the rapid cooling and the operation are facilitated, the compatibility with the freezing protection solution is better, and the recovery rate of gametes or embryos is improved; the inserted rod is not easy to fall off from the carrying rod, so that the safety of gametes or embryos is improved; the outer sleeve is made of low temperature resistant material, can adapt to low temperature of minus 196 ℃ in liquid nitrogen, and has unchanged hardness when meeting cold; can effectively protect gametes or embryos and avoid direct exposure of the gametes or embryos in a liquid nitrogen environment.
Description
[ technical field ] A method for producing a semiconductor device
The utility model relates to the technical field of medical instruments, in particular to a closed vitrification freezing carrier.
[ background of the utility model ]
In the assisted reproduction technology, when in vitro fertilization-embryo transplantation is carried out, after the embryo in a fresh period is transplanted, the rest embryo is frozen and stored, firstly, the embryo is treated by vitrification freezing protective solution, then the embryo is loaded on a freezing carrying rod, and then the embryo is put into liquid nitrogen for long-term storage. The prior vitrification freezing carrier has two types, one is an open type freezing carrier; another class is closed frozen carriers. Open freezing carriers have two disadvantages: 1. the embryo is directly exposed in a liquid nitrogen environment without disinfection and sterilization, so that the risk of directly damaging the embryo exists; 2. for patients with infectious diseases themselves, their embryos are also exposed to such exposure, and the embryos of this part of the patient are stored in the same container, and there may be a risk of cross-infection with each other. The closed type freezing carrier also has two defects: the recovery rate of the closed freezing carrier embryo is lower than that of the open carrier embryo, and the reasons for the low recovery rate are as follows: 1. the slide glass for loading the embryo is thicker and has slow cooling rate, so that ice crystals are formed in embryo cells to cause damage to the embryo; 2. the compatibility of the slide carrying the embryo and the cryoprotective solution is poor, and in order to reduce the volume of liquid drops on the slide, the operation time is prolonged, and the embryo can be damaged.
Patent document CN204796590U, publication date: 2015.11.25 discloses a closed freezing carrier, at least one liquid seal ring is established to the cover on the connecting portion, and the sleeve pipe is one end opening one end confined tubular structure, connecting portion and cryopreserving portion get into and overlap at the sleeve pipe by sheathed tube opening, and the liquid seal ring card is established at the sheathed tube open end, and liquid seal ring, connecting portion, cryopreserving portion and sheathed tube bottom space constitute a confined freezing space, and the liquid of adhesion on liquid seal ring fills all gaps of liquid seal ring and sleeve pipe junction, and it is right that liquid solidifies the realization after freezing confined freezing space's is sealed. The advantages are that: the biological sample does not contact with common liquid nitrogen with potential microbial hazard when being frozen, so that the operation of freezing loading, marking, storing, distinguishing, taking out, unfreezing and the like is simple and convenient, the operation is safer, the space occupied by the carrier device is reduced more obviously, and the storage capacity of each liquid nitrogen tank is increased by about 3-8 times. The device improves the safety and the application efficiency of the storage space, but cannot solve the problem of low recovery rate of the closed freezing carrier embryos.
Patent document CN205695272U, publication date: 2016.11.23 discloses a freezing carrier, which comprises a sleeve and a long rod-shaped core rod, wherein the lower end of the core rod is provided with a freezing part for placing biological materials to be frozen, the freezing part is inserted into the sleeve, the upper end of the core rod extends out of the sleeve, the sleeve is in a cylinder shape, a metal counterweight is fixed on the inner side of the lower end of the sleeve, a through hole is arranged in the metal counterweight in a penetrating way along the vertical direction, the through hole can communicate an opening at the lower end of the sleeve with an inner cavity on the upper side of the metal counterweight in the sleeve, the sleeve between the metal counterweight and the freezing part is filled with a filler capable of absorbing water, and a distance is reserved between the freezing part and the filler. The advantages are that: the problem of current closed freezing carrier survival rate when preserving the biomaterial is low is solved, freezing fast and biomaterial survival rate is high. The device has the advantages of high freezing speed and high survival rate of biological materials, and also does not solve the problem of low recovery rate of the closed freezing carrier embryos.
In summary, there is a need for a closed vitrification freezing carrier that can not only protect gametes or embryos, avoid direct exposure to liquid nitrogen, but also reduce the thickness of slides carrying gametes or embryos, improve the compatibility of slides with a freezing protection solution, accelerate the cooling rate, and increase the recovery rate of gametes or embryos.
[ summary of the utility model ]
The utility model aims to provide a closed type vitrification freezing carrier which can not only protect gametes or embryos and prevent gametes or embryos from being directly exposed in a liquid nitrogen environment, but also reduce the thickness of a slide glass for loading gametes or embryos, improve the compatibility of the slide glass and a freezing protection solution, accelerate the cooling rate and improve the recovery rate of gametes or embryos.
In order to achieve the purpose, the utility model adopts the technical scheme that:
a closed vitrification carrier is provided with a carrier; the carrier is formed by sleeving an outer sleeve, an inserting rod, a carrying rod and a slide glass; one end of the outer sleeve is open and the other end is closed, and metal is arranged on the inner side of the closed end; the inserted rod is a solid rod, and the diameter of the inserted rod is reduced at three quarters of the length; the slide bar is connected with the slide glass, the front half part is a tubular slide bar, and the rear half part is a sheet slide glass with radian; the narrower part of the inserted link is sleeved in the carrying link; the inserted bar, the carrying bar and the carrying sheet are sleeved in the outer sleeve; the outer sleeve completely sleeves the inserted rod, the carrying rod and the carrying sheet inside.
As a preferred example, the outer sleeve is made of low-temperature-resistant material, and the diameter of the opening end is larger than the pipe diameter.
As another preferred example, the carrier rod and the slide are the same length.
As a further refinement, the outer sleeve is provided with a closure cap.
The utility model has the advantages that:
1. the novel closed vitrification freezing carrier is provided, the thickness of the slide glass part is thinner than that of the prior art, rapid cooling is facilitated, the compatibility with a freezing protection solution is better, the operation is more facilitated, and the gamete or embryo resuscitation rate is improved.
2. Different from the prior art, the upper end of the slide glass part is tubular and is sleeved on the inserted rod, the inner diameter of the sleeve is equal to the diameter of the lower end part of the inserted rod, the sleeve is not easy to fall off, and the safety of gametes or embryos is improved.
3. The outer sleeve is made of low temperature resistant material, can adapt to low temperature of minus 196 ℃ in liquid nitrogen, and the hardness of the outer sleeve can not change when meeting cold.
4. The device can effectively protect gametes or embryos and prevent the gametes or embryos from being directly exposed in a liquid nitrogen environment.
[ description of the drawings ]
FIG. 1 is a schematic structural diagram of an outer sleeve of a closed vitrification freezing carrier according to the present invention.
FIG. 2 is a schematic diagram of a plug-in rod structure of a closed vitrification freezing carrier according to the present invention.
FIG. 3 is a schematic diagram of the structure of a slide rod and a slide of a closed vitrification freezing carrier according to the present invention.
FIG. 4 is a schematic structural diagram of a closed vitrification freezing carrier according to the present invention.
FIG. 5 is a schematic view of another jacket structure of an enclosed vitrification freezing medium of the present invention.
[ detailed description ] embodiments
The utility model is further described with reference to the following examples and with reference to the accompanying drawings.
The reference numerals and components referred to in the drawings are as follows:
| 1. |
2. |
| 3. |
4. Loading |
| 5. |
6. Metal |
| 7. Sealing cover |
Example 1
Referring to fig. 1-4, fig. 1 is a schematic structural view of an outer sleeve of a closed type vitrification frozen carrier of the present invention, fig. 2 is a schematic structural view of an insertion rod of a closed type vitrification frozen carrier of the present invention, fig. 3 is a schematic structural view of a carrying rod and a carrying sheet of a closed type vitrification frozen carrier of the present invention, and fig. 4 is a schematic structural view of a closed type vitrification frozen carrier of the present invention. The closed vitrification carrier is provided with a carrier 1; the carrier 1 is formed by sleeving an outer sleeve 2, an inserted link 3, a carrying link 4 and a carrying sheet 5; one end of the outer sleeve 2 is open and the other end is closed, the diameter of the open end is larger than the diameter of the pipe, and metal 6 is arranged in the closed end; the inserted rod 3 is a solid rod, and the diameter of the inserted rod is reduced at three quarters; the carrier rod 4 is connected with the slide glass 5, the front half part is a tubular carrier rod 4, and the rear half part is a sheet slide glass 5 with radian; the length of the carrying rod 4 is the same as that of the carrying sheet 5; the narrower part of the inserted bar 3 is sleeved in the carrying bar 4; the inserted bar 3, the carrying bar 4 and the carrying sheet 5 are sleeved in the outer sleeve 2; the outer sleeve 2 completely sleeves the inserted rod 3, the carrying rod 4 and the slide 5.
The use method and the principle of the closed vitrification freezing carrier are as follows: in the case of in vitro fertilization-embryo transfer, fresh cycle embryos are cryopreserved for the remaining embryos after transfer. The embryo is first put into the freezing protection solution for vitrification freezing protection solution treatment. The cryoprotectant solution containing the embryos is then carefully transferred to slide 5. After the inserting rod 3 is inserted into the carrying rod 4, the inserting rod 3, the carrying rod 4 and the slide glass 5 are sleeved in the outer sleeve 2, and the opening end of the outer sleeve 2 is sealed after the sleeving is finished. And finally, putting the frozen carrier with the sealed opening into liquid nitrogen for long-term storage.
It should be noted that the freezing carrier slide 5 of the utility model is thinner than the prior art, has a sheet-shaped structure with radian, has a diameter of 1.2mm and a length of 1.5cm, and the thinner slide 5 is beneficial to rapid cooling, has better compatibility with the freezing protection solution, is more beneficial to operation and improves the recovery rate of gametes or embryos. Meanwhile, the carrying rod 4 connected with the slide 5 is tubular, the inner diameter is 1.5mm, the total length of the carrying rod 4 and the slide 5 is 6cm, the inner diameter of the carrying rod 4 is equal to the diameter of the lower end part of the inserting rod 3, the carrying rod 4 is not easy to fall off when being sleeved on the inserting rod 3, and the safety of gametes or embryos is improved. The outer sleeve 2 is made of low temperature resistant material, the lowest temperature can reach 196 ℃ below zero, the hardness is not changed after the outer sleeve is cooled, the diameter of the opening end is 3.2mm, the pipe diameter is 2.2mm, and the length is 13.3cm, so that gametes or embryos can be more effectively protected, and the gametes or embryos are prevented from being directly exposed in a liquid nitrogen environment. The inserted rod 3 is 4cm long, the narrow part is 1cm long, and the total length after the inserted rod 3 is connected with the carrying rod 4 and the slide 5 is 9.3 cm.
Example 2
Referring to fig. 5, fig. 5 is a schematic view of another jacket structure of an enclosed vitrification freezing carrier according to the present invention. The structure of this embodiment 2 is basically the same as that of embodiment 1 except that a sealing lid 7 is added. The function is as follows: after the device is used, the inserted rod 3, the carrying rod 4 and the carrying sheet 5 are sleeved in the outer sleeve 2 to be sealed, so that the inside of the device can be kept clean, the whole device can be completely stored together, and the device can be conveniently taken next time.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and additions can be made without departing from the principle of the present invention, and these should also be considered as the protection scope of the present invention.
Claims (4)
1. A closed vitrification carrier is characterized in that a carrier (1) is provided; the carrier (1) is formed by sleeving an outer sleeve (2), an inserted bar (3), a carrying bar (4) and a slide glass (5); one end of the outer sleeve (2) is open and the other end is closed, and the inner side of the closed end is provided with metal (6); the inserted rod (3) is a solid rod, and the diameter of the inserted rod is reduced at three quarters of the length; the carrier rod (4) is connected with the slide glass (5), the front half part is a tubular carrier rod (4), and the rear half part is a sheet slide glass (5) with radian; the narrower part of the inserted bar (3) is sleeved in the carrying bar (4); the inserted rod (3), the carrying rod (4) and the carrying sheet (5) are sleeved in the outer sleeve (2); the outer sleeve (2) completely sleeves the inserted bar (3), the carrying bar (4) and the carrying sheet (5).
2. A closed vitrified frozen carrier according to claim 1 wherein the outer sleeve (2) is made of a material resistant to low temperatures and the diameter of the open end is larger than the diameter of the tube.
3. The enclosed vitrification cryocarrier of claim 1 wherein the carrier rod (4) and the slide (5) are the same length.
4. A closed vitrification frozen carrier in accordance with claim 1 characterized in that the outer sleeve (2) is provided with a closure cap (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122361794.2U CN215913099U (en) | 2021-09-28 | 2021-09-28 | Closed vitrification carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122361794.2U CN215913099U (en) | 2021-09-28 | 2021-09-28 | Closed vitrification carrier |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215913099U true CN215913099U (en) | 2022-03-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122361794.2U Active CN215913099U (en) | 2021-09-28 | 2021-09-28 | Closed vitrification carrier |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215913099U (en) |
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2021
- 2021-09-28 CN CN202122361794.2U patent/CN215913099U/en active Active
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