CN202127748U - Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank - Google Patents
Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank Download PDFInfo
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- CN202127748U CN202127748U CN201120189641U CN201120189641U CN202127748U CN 202127748 U CN202127748 U CN 202127748U CN 201120189641 U CN201120189641 U CN 201120189641U CN 201120189641 U CN201120189641 U CN 201120189641U CN 202127748 U CN202127748 U CN 202127748U
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- liquid nitrogen
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- cryopreservation
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Abstract
The utility model provides a simple auxiliary device for sperm cryopreservation by a liquid nitrogen tank, which consists of a net-shaped suitcase, a lifting rod, a net-shaped suitcase cover, a thermocouple probe and a sliding buckle; the simple auxiliary device is characterized in that the lifting rod is connected with one side of the net-shaped suitcase, the sliding buckle is arranged on the lifting rod, the net-shaped suitcase cover which is hinged with the net-shaped suitcase by a buckle ring is arranged at the upper end of the net-shaped suitcase, the net-shaped suitcase cover is provided with a small hole, the thermocouple probe penetrates through the small hole and a freezing pipe to be arranged in the net-shaped suitcase. The simple auxiliary device for the sperm cryopreservation by the liquid nitrogen tank precisely controls the height of a sample placed in the liquid nitrogen tank, is beneficial for improving the repeatability of the cryopreservation effect of the liquid nitrogen tank, improves the uniformity and the stability of the cryopreservation effect, and monitors the temperature variation of the sample environment in the cryopreservation process in real time; the buckle ring of the net-shaped cover facilitates the storing and taking of the sample, thereby improving the safety and convenience of the cryopreservation of the liquid nitrogen tank; the heat insulating plastic of the lifting rod is convenient for operating, and reduces the temperature effect possibly generated when a hand holds the lifting rod.
Description
Technical field
The utility model relates to and is used for freezing preservation servicing unit, relates in particular to a kind of simple and easy servicing unit that is used for liquid nitrogen container substep cryopreservation sperm.
Technical background
It is the experimental technique of extensive use in the present biological study that sperm freezing is preserved, significant at fish quality saving, genetic breeding and seed producer mask.Preserve and sperm bank foundation through sperm freezing; Not only can rare and endangered fish germplasm long preservation be got off; Help the protection of bio-diversity, but also can the sperm material be provided anywhere or anytime, make things convenient for carrying out of genetic breeding research greatly for researchs such as fish gynogenesiss.In addition, also can solve the space-time restriction that male and female fish maturation is asynchronous in the parent population reproductive process, geographic isolation causes being difficult between some fish mating, for breeding production facilitates.
At present; The freezing two kinds of approach of preserving commonly used, a kind of is to adopt the programmed cooling instrument to carry out, the amount of liquid nitrogen that gets into frozen sample cavity through computer control; Come the variations in temperature in strict control cooling and the temperature-rise period; As long as the certain cooling process of input, frozen sample is placed on lowers the temperature freezingly in the cavity of cooling instrument, just can obtain stable freezing preservation effect.Yet the current procedure cooling instrument belongs to precision instrument, costs an arm and a leg, and is used for carrying out specially the unit of cryobiology research more, and for the unit that simple frozen sperm is used, its use has limitation.Second kind is exactly the frozen method of liquid nitrogen container substep, and it is in line with a kind of simple and easy sperm cryopreservation method practical, application demand exploitation easily.Its principle is to utilize in the liquid nitrogen container, and the thermograde difference of differing heights on the liquid level realizes that the progressively cooling of sample in the liquid nitrogen container is freezing.In the operation, frozen pipe from top to bottom is placed on differing heights in the liquid nitrogen container step by step, and stops special time, thereby reach progressively cool effect, wherein the position of each step placement of sample and the time of staying are that freezing and storing method needs clear and definite key element.This method is because device simple, easy and simple to handle has obtained extensive use in research and production.Yet this method is not owing to there is normalized operation assisting device; A lot of situation all are that the operator gathers materials on the spot; Therefore exist operating process to exist bigger individual difference in operation, the difficult stable control of the interior temperature of liquid nitrogen container, sample to throw in deficiencies such as lack of standardization, influenced the stability of freezing preservation effect.Need develop simple and easy servicing unit to this method for this reason, increase monitoring and standard, thereby improve the practicality and the stability of this method liquid nitrogen container substep temperature-fall period through servicing unit.
Summary of the invention
The purpose of the utility model is to solve present domestic prior art defective and deficiency in the field; Designed a kind of simple and easy servicing unit that is used for the freezing preservation sperm of liquid nitrogen container, by the variations in temperature of the thermocouple monitoring refrigerating process that inserts the netted suitcase of sample; Length scale on the handle is controlled the height and position that sample is put into liquid nitrogen container; The heatpiston of handle is convenient to experimental implementation, reduces issuable temperature effect when holding simultaneously; Netted suitcase is placed frozen pipe sample, and the netted suitcase of whole sample is the hollow out device, no matter frozen pipe netted suitcase where can both guarantee the uniformity of medium temperature, thereby make the frozen cooling effect more stable; Prevent by netted suitcase lid that frozen pipe is floating and go out cavity, make more convenient operation, safety.Place netted suitcase central thermocouple probe, and monitor in real time, can evenly reflect the temperature environment of sample, effectively monitor the variations in temperature of temperature-fall period through digital display; Scale label on the handle can accurately reflect the position of sample in liquid nitrogen container, and helps the stability of repetitive operation.Improve the practicality and the stability of freezing method.
The purpose of the utility model is realized by following technical scheme, has developed a kind of simple and easy servicing unit that is used for the freezing preservation sperm of liquid nitrogen container, and the simple and easy servicing unit of the freezing preservation sperm of this liquid nitrogen container is by netted suitcase 1; Shadoof 2; Netted case lid 3, thermocouple probe 4, slip buckle 5 are formed, and it is characterized in that: described shadoof 2 is connected a side of netted suitcase 1; Slip buckle 5 is arranged on the shadoof 2; Netted suitcase 1 upper end is provided with netted case lid 3 through clasp 6 and netted suitcase 1 interlock, on the netted case lid 3 aperture 9 is set, and thermocouple probe 4 passes aperture 9 and is arranged in the netted suitcase 1 with cryovial 8.
Described shadoof 2 is connected a side of netted suitcase 1, and vertically upward, shadoof 2 is provided with length scales, and hook is arranged in shadoof 2 upper ends, and rubber blanket 7 is set in hook.
Described netted suitcase 1 is cylinder shape, and hollow out in the case is made with bonding jumper, and bottom surface, netted case lid 3 and netted suitcase 1 casing reticulate.
The utility model has the advantages that: accurately control the height and position that sample is put into liquid nitrogen container, help improving the repeatability of the freezing preservation effect of liquid nitrogen container; Homogeneity, the stability of frozen effect have been improved; Monitor the variations in temperature of sample environment in the refrigerating process in real time; Net cage lid clasp has made things convenient for the sample access, has improved safety, the convenience of liquid nitrogen container freezing and storing method; The heatpiston of handle is convenient to operation, reduces issuable temperature effect when holding simultaneously.
Description of drawings
Fig. 1 is the structural representation of the utility model.
Specific embodiment:
The utility model has been developed a kind of simple and easy servicing unit that is used for the freezing preservation sperm of liquid nitrogen container, takes following steps:
The simple and easy servicing unit of the freezing preservation sperm of this liquid nitrogen container is by netted suitcase 1, shadoof 2, netted case lid 3; Thermocouple probe 4, slip buckle 5 are formed; It is characterized in that: described shadoof 2 is connected a side of netted suitcase 1, and slip buckle 5 is arranged on the shadoof 2, and netted suitcase 1 upper end is provided with netted case lid 3 through clasp 6 and netted suitcase 1 interlock; On the netted case lid 3 aperture 9 is set, thermocouple probe 4 passes aperture 9 and is arranged in the netted suitcase 1 with cryovial 8.
Described shadoof 2 is connected a side of netted suitcase 1, and vertically upward, shadoof 2 is provided with length scales, and hook is arranged in shadoof 2 upper ends, and rubber blanket 7 is set in hook.
Described netted suitcase 1 is cylinder shape, and hollow out in the case is made with bonding jumper, and bottom surface, netted case lid 3 and netted suitcase 1 casing reticulate.
In conjunction with accompanying drawing and embodiment, when using the utility model, open case lid, frozen pipe is put into casing, and buckle the lid snap close.Thermocouple is inserted lid central authorities, and open the digital display power supply.The plastics Heat-insulated handle position of the portable handle of operator is come according to the scale on the different substep stop place contrast handles, mixes up the fixedly position of card; Be placed on liquid nitrogen container mouth edge; After the time stop according to the operation requirement, promptly adjustable card carries out the frozen cooling process in second step to next position; And the like, until at last sample cavity directly being immersed liquid nitrogen inside.The variations in temperature of sample can show that the operator can monitor at any time through thermocouple measurement and by numeral in the whole operation process.Experiment showed, that this is the servicing unit of the freezing preservation sperm of a kind of effective liquid nitrogen substep.
In addition, the utility model and not meaning that by sketch map and specification limits to, and under the prerequisite that does not break away from design aim and principle thereof, can change to some extent.
Claims (3)
1. simple and easy servicing unit that is used for the freezing preservation sperm of liquid nitrogen container; By netted suitcase (1), shadoof (2), netted case lid (3); Thermocouple probe (4), slip buckle (5) are formed; It is characterized in that: described shadoof (2) is connected a side of netted suitcase (1), and slip buckle (5) is arranged on the shadoof (2), and netted suitcase (1) upper end is provided with netted case lid (3) through clasp (6) and netted suitcase (1) interlock; Aperture (9) is set on the netted case lid (3), and thermocouple probe (4) passes aperture (9) and cryovial (8) is arranged in the netted suitcase (1).
2. the simple and easy servicing unit that is used for the freezing preservation sperm of liquid nitrogen container according to claim 1; It is characterized in that: described shadoof (2) is connected a side of netted suitcase (1); Vertically upward; Shadoof (2) is provided with length scales, and hook is arranged in shadoof (2) upper end, and rubber blanket (7) is set in hook.
3. the simple and easy servicing unit that is used for the freezing preservation sperm of liquid nitrogen container according to claim 1; It is characterized in that: described netted suitcase (1) is cylinder shape; Hollow out in the case is made with bonding jumper, and bottom surface, netted case lid (3) and netted suitcase (1) casing reticulate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120189641U CN202127748U (en) | 2011-05-25 | 2011-05-25 | Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120189641U CN202127748U (en) | 2011-05-25 | 2011-05-25 | Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202127748U true CN202127748U (en) | 2012-02-01 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201120189641U Expired - Fee Related CN202127748U (en) | 2011-05-25 | 2011-05-25 | Simple auxiliary device for sperm cryopreservation by liquid nitrogen tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202127748U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444699A (en) * | 2013-08-01 | 2013-12-18 | 吕祁峰 | Miniature carrier system for cryopreservation of biological materials, bucket and cryopreservation method |
| CN107352165A (en) * | 2017-08-30 | 2017-11-17 | 广西壮族自治区农业科学院经济作物研究所 | The access device of single sample box in a kind of liquid nitrogen container |
| CN107438744A (en) * | 2015-03-30 | 2017-12-05 | 布鲁克斯自动化公司 | Automatic low-temp stocking system |
| CN108514067A (en) * | 2018-03-29 | 2018-09-11 | 中国农业科学院蜜蜂研究所 | A kind of cryopreservation device and its cryopreservation methods for royal jelly |
-
2011
- 2011-05-25 CN CN201120189641U patent/CN202127748U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103444699A (en) * | 2013-08-01 | 2013-12-18 | 吕祁峰 | Miniature carrier system for cryopreservation of biological materials, bucket and cryopreservation method |
| CN103444699B (en) * | 2013-08-01 | 2015-07-29 | 吕祁峰 | A kind of miniature base system, well-bucket and the cryopreservation methods frozen for biomaterial |
| CN107438744A (en) * | 2015-03-30 | 2017-12-05 | 布鲁克斯自动化公司 | Automatic low-temp stocking system |
| CN107352165A (en) * | 2017-08-30 | 2017-11-17 | 广西壮族自治区农业科学院经济作物研究所 | The access device of single sample box in a kind of liquid nitrogen container |
| CN107352165B (en) * | 2017-08-30 | 2020-01-17 | 广西壮族自治区农业科学院经济作物研究所 | Access device of single sample box in liquid nitrogen jar |
| CN108514067A (en) * | 2018-03-29 | 2018-09-11 | 中国农业科学院蜜蜂研究所 | A kind of cryopreservation device and its cryopreservation methods for royal jelly |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120201 Termination date: 20130525 |