CN104642299A - Vitrification freezing carrier system - Google Patents
Vitrification freezing carrier system Download PDFInfo
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- CN104642299A CN104642299A CN201310596542.5A CN201310596542A CN104642299A CN 104642299 A CN104642299 A CN 104642299A CN 201310596542 A CN201310596542 A CN 201310596542A CN 104642299 A CN104642299 A CN 104642299A
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- freezing
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- rod
- cover
- protection cylinder
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Abstract
The invention provides an embryo vitrification freezing carrier system, which includes a freezing unit. The freezing unit includes a freezing rod and a freezing rod casing pipe. The freezing rod comprises a rod body, the rod body includes a lower extension part and an upper handle part, and the lower end of the extension part is connected to a cryopreservation sheet. The freezing rod casing pipe is a hollow structure, and at least the upper end is provided with an opening for communicating the hollow structure with the external environment. The extension part of the freezing rod is inserted into the hollow structure of the freezing rod casing pipe to form a loading unit. The outer diameter of the extension part is smaller than the outer diameter of the handle part. The outer diameter of the freezing rod casing pipe compose the largest outer diameter of the loading unit. The system provided by the invention exponentially reduces the space occupied by trace biological samples freezing on the basis of not influencing or even increasing the use convenience, greatly saves the floor space needed by freezing storage, and is especially suitable for cryopreservation of trace biomaterials like vitrified frozen embryos, etc.
Description
Technical field
The present invention relates to a kind of carrier system frozen for biomaterial, particularly relate to a kind of vitrified frozen vector system used when supplementary reproduction medical domain carries out the glass freezing of human embryos or ovum.
Background technology
At present, known embryo vitrification freezing carrier has many kinds, and wherein open frozen, namely embryo directly contacts the frozen of liquid nitrogen, becomes the optimised form of embryo vitrifying freeze because cooling is fast.Its carrier usually adopted is the freezing rod type that front end has frozen carrier sheet (being called for short frozen), and it is simple to operate fast and efficient.
But current embryo vitrifying freeze bar and accordingly Load System storage area application efficiency are low.This reason is wherein: comprise and carry a bar (one end has frozen), carry bar cover, carry bar cylinder, carry bar cylinder cap and carry bar tub system.Fig. 1 is conventional carriers system schematic, forms by carrying bar 1 ', carrying bar cover 2 ', carry bar cylinder 3 ', carry bar cylinder cap 4 ' and carrying bar tub 5 '.As shown on the left-hand side of the figure, carry bar 1 ' front end to insert and carry in bar cover 2 ', then be loaded into together and carry in bar cylinder 3 ', and carry bar cylinder 3 ' and carried in bar tub 5 ' by clamping, its top has the bar cylinder cap 4 ' that carries by carrying bar tub 5 ' clamping to cover, and prevents from carrying dropping out of bar 1 '.Wherein carry on bar frozen for placing embryo, bar cover uploaded by its one end quilt cover placing embryo, do not damage to protect this section by external force; Carry bar cylinder then to carry bar for loading and carry bar cover; An aluminium alloy frame is also had namely to carry bar tub, for clamping year bar cylinder and year bar cylinder cap (carrying bar cylinder cap not drop out outside cylinder for the protection of article in cylinder).Carry on bar and can post biomaterial information labels.Carry bar tub upper end and can stick the information labels such as frozen position, form a frozen unit.Generally the multiple samples deriving from same patient being carried bar is loaded in same or multiple frozen unit, and the embryo in different patient source can not load in mixture in same frozen unit, is beneficial to identity marks and resolution.
As above carry bar tub and clamping carry bar cylinder and carry bar cylinder cap not only to have an appointment the thickness of 1-1.5 cm diameter, also have about 29 centimetre lengths altogether.In this covering device, what generation maximum space took has two aspects: (1) carries a bar tub, because it must than carry bar cylinder greatly just can hold clamping year bar cylinder and with carry bar cylinder etc. large carry bar cylinder cap, and this clamping necessity is that will fix a year bar cylinder cap makes it to be in a year bar cylinder openend, do not drop out to protect the bar that carries carried in bar cylinder; (2) being carry bar cover, because it must the one end of bar being placed embryo is large just can put this end than carrying, thus usually creating than carrying the thicker space hold of bar.This makes to carry bar cylinder must have comparatively thick (about 1 centimetre) internal diameter for meeting usual useful load (6-8 embryo needs 3-4 root to carry bar).
In general, the embryo of a patient at least needs to carry bar tub with one, need according to transplanting, the frozen amount that every root carries bar is 1-3 piece of embryo, and a quantity of carrying bar wound packages year bar is between 1 to 4, namely when client need frozen about 8 and following embryo time, it need take a support bar tub, then needs to take 2 or more carry bar tub more than 8.Loading when this Load System can facilitate freezing, finds rapidly when also conveniently thawing and carries bridge and carry bar from carrying bridge to extract out from liquid nitrogen container.But, although this system uses conveniently upper, occupy many spaces.In the liquid nitrogen container of 47 liters of 10 well-buckets, each well-bucket only can put into 26-28 support bar tub, at least needs a support bar tub to calculate, then the embryo of frozen at most 28 patients of each well-bucket with each patient.Then the liquid nitrogen container of 47 liters of 10 well-buckets can deposit at most the embryo of 280 patients.Concerning a relatively large supplementary reproduction center, if on average monthly get the ovum cycle more than 500, even if consider to count the space that embryo of thawing discharges in, its liquid nitrogen container needed for newly-increased frozen embryo may need increase by average every month, within several years, gets off and will cause taking of extra space.This causes restriction to the development of a supplementary reproduction mechanism.Therefore, current micro-Frozen Biological device and the space efficiency utilization of corresponding loading technology relatively low, also cannot meet the frozen and development of the sample of large-scale.
On the other hand, current to carry a bar tub be aluminum alloy material due to it, freezing or thaw in operation, can produce and freeze to the condensation of water in air vapour, hide label, cause the inconvenience of operation because label segment exposes liquid nitrogen certain hour.
Summary of the invention
In order to overcome existing micro-Frozen Biological device and the corresponding deficiency of loading technology in storage area utilization ratio thereof, the invention provides a kind of carrier system frozen for micro-biomaterial and loading technology thereof, with guarantee freezing loading, mark, storage, resolution and taking-up and to thaw etc. each simple and convenient while, the space shared by carrier arrangement can also be reduced more significantly, make each liquid nitrogen container amount of storage (embryo vitrifying freeze for supplementary reproduction then refers to the case load of storing samples) increase about 3-4 doubly.
The invention provides a kind of vitrified frozen vector system, comprise freezing unit, described freezing unit comprises freezing bar and freezing rod bush, wherein,
Described freezing bar comprises the body of rod, and the described body of rod comprises the extension of bottom and the shank on top, and the lower end of described extension is connected with frozen;
Described freezing rod bush is hollow structure, and at least upper end is provided with the opening be communicated with external environment condition by described hollow structure; The opening of extension through freezing rod bush upper end of described freezing bar is inserted in the hollow structure of freezing rod bush, forms load units;
The external diameter of described extension is less than the external diameter of shank; Described freezing rod bush external diameter forms load units maximum outside diameter.
Preferably, described frozen is strip small pieces, and its cross section is circular arc spill.
Preferably, described extension is provided with shank junction and overlaps solid section, the maximum outside diameter of the solid section of described cover equals the internal diameter of freezing rod bush opening part.。
Preferably, one or two in the hollow structure inner surface that the outer surface of the extension of the body of rod of at least freezing bar and lengthening are overlapped is matsurface, to make to produce frictional force between the extension of the body of rod of freezing bar and the hollow structure inner surface carrying bar extending sleeve.
Further preferably, described extension is provided with shank junction and overlaps solid section, the maximum outside diameter of the solid section of described cover equals the internal diameter of freezing rod bush opening part.
Preferably, the length of the solid section of described cover is 0.1-10mm.
One or two in the hollow structure inner surface of the solid section of described cover and lengthening cover is matsurface, to make to produce frictional force between the shank of the body of rod of freezing bar and the hollow structure inner surface carrying bar extending sleeve.
The solid section of described cover can be shank or the extension part of the body of rod of freezing bar.
The solid section of described cover is preferably progressively reduced by the direction of shank to extension, is more preferably slightly progressively reduced by the direction of shank to extension.
In a preferred embodiment of the invention, described freezing rod bush is comparatively thick, directly uses as protection cylinder.
Preferably, described freezing unit is only containing 1 load units.
Preferably, the external diameter >=3mm of the extension of described freezing bar, is more preferably 3-10mm, is more preferably 4-9mm, be more preferably 5-8mm, be more preferably 6-7mm.
Preferably, described freezing unit also comprises lengthening cover, and described lengthening cover is hollow structure at least partly, bottom end opening, and the hollow structure of described lengthening cover is communicated with the bottom end opening that lengthening is overlapped; The top of the shank of the body of rod of described freezing bar or be all inserted into from the bottom end opening lengthening cover lengthen cover hollow structure in.
Preferably, the shank of described freezing bar external diameter or hold freezing bar shank lengthening cover external diameter consistent with the load units maximum outside diameter that described freezing rod bush external diameter is formed.
Preferably, described freezing rod bush in the reduced diameter at the position that the extension with described freezing bar is socketed, but is greater than the external diameter of freezing bar shank, and described lengthening cover is socketed on the outer wall reduced of described freezing rod bush.
Further preferably, the body of rod of described freezing bar is hollow, is communicated with the inside of freezing rod bush and lengthens the hollow structure of cover.
Further preferably, described lengthening cover is hollow tubular, upper end closed, and on the described epimere sidewall lengthening cover and/or top is provided with ventilation hole.
Preferably, the bottom of described freezing rod bush is provided with weight.
Described weight is preferably metal material.
In a preferred embodiment of the invention; described freezing unit contains one or more load units; described freezing unit also comprises bottom end closure and the protection cylinder of the hollow of upper end open; the external diameter of described protection cylinder is that 3-10mm(is more preferably 4-9mm, is more preferably 5-8mm, is more preferably 6-7mm); the external diameter < 3mm of described freezing bar, described protection cylinder is provided with at least one window load units can put into and take out.
In the present invention's preferred embodiment, the distance of described freezing bar extension between the outer surface and handle outer surface at shank place equals the wall thickness of freezing bar loop bar opening part; The upper end of the body of rod of described freezing bar is provided with label and sampling section, described label and the sampling Duan Weiwei thin bar thinner than the body of rod or the flat-shaped body narrower than the body of rod.
Described label sampling section may be used for pasting label, is convenient to carry out mark identification.
Described label sampling section can also be convenient to gripping.
Described label and sampling section have tubulose or pointed, can by the tapering tweezers gripping in front end, or the little bar tubular end being provided with front end pointed is fixed and extracts, or are provided with the tubular little bar in front end and are inserted in pointed end and fix and extract.
The shape of described label sampling section can be circular tube shaped, flat tubulose, strip of sheet or splinter shape etc.
Preferably, the upper end open of described protection cylinder is directly as the window put into by load units and take out, and the window by described protection cylinder is arranged on protection cylinder top.
Preferably, described freezing unit also comprises lengthening cover, and described lengthening cover is hollow structure at least partly, bottom end opening, and the hollow structure of described lengthening cover is communicated with the bottom end opening that lengthening is overlapped; Described lengthening cover lower end reduces, and be inserted in protection cylinder, lengthen the internal diameter that the external diameter not reducing part overlapped is greater than protection cylinder top end opening, thus fixing lengthening is overlapped from the top end opening of protection cylinder.
Preferably, the length of described load units is less than the bottom of protection cylinder when lengthening cover is fixed on protection cylinder to lengthening the length of overlapping lower end.
Further preferably, described lengthening cover is hollow tubular, upper end closed, and on the described epimere sidewall lengthening cover or top is provided with ventilation hole.
Preferably, the bottom of described protection cylinder is provided with weight.
Described weight is preferably metal material.
Preferably, the window of described protection cylinder is arranged on the side of protection cylinder, in straight strip or oblique strip.
Preferably, be provided with lateral partitions, protection cylinder is divided into multistage in described protection cylinder, each section forms protected location, and wherein, the top end opening of protected location topmost forms window, and/or sidewall is provided with at least one window; The sidewall of other each protected locations is provided with the window of at least one.
The invention has the beneficial effects as follows, making, not affecting the space basis even more increasing ease of use decreased exponentially shared by micro-Frozen Biological, significantly to have saved the floor space needed for stored frozen.Be particularly useful for frozen as glass freezing embryo etc. of micro-biomaterial.
Accompanying drawing explanation
Fig. 1 is for being conventional carriers system and loading schematic flow sheet;
Fig. 2 is the vitrified frozen vector system in the first embodiment of the present invention;
Fig. 3 is that bar schematic flow sheet is carried in vitrified frozen vector system in the second embodiment of the present invention and loading and taking-up;
Fig. 4 is that bar schematic flow sheet is carried in vitrified frozen vector system in the third embodiment of the present invention and loading and taking-up.
Embodiment
Below in conjunction with accompanying drawing, by specific embodiment, the detailed description of shape is entered to the present invention.
Embodiment 1
The vitrified frozen vector system that the present embodiment provides, comprises freezing bar 1, freezing rod bush 2 and lengthens cover 3, and wherein, the external diameter of freezing rod bush 2 increases, and directly plays a part to protect cylinder.
Freezing bar 1 comprises the body of rod, the extension 11 of the described body of rod by bottom and the shank 12 on top, the lower end of extension 11 is connected with frozen 13, frozen 13 is strip small pieces, it is transversal to being circular arc spill, such as, can be that the sidewall of the transparent or semitransparent plastic straw of hard straight thin-walled cuts the strip small pieces cut in longitudinal rule;
Freezing rod bush 2 is hollow structure, top closure, and upper end is provided with the opening be communicated with in external environment condition by described hollow structure; The opening of extension 11 through freezing rod bush 2 upper end of freezing bar is inserted in the hollow structure of freezing rod bush 2, forms load units.
The external diameter of extension 11 is less than the external diameter of shank 12, and the distance between the outer surface of extension 11 at shank 12 place and shank 12 outer surface equals the wall thickness of freezing bar loop bar 2 opening part.
The outer surface of the extension 11 of the body of rod of at least freezing bar and lengthen in the hollow structure inner surface of cover 2 one or two be matsurface, to make to produce frictional force between the hollow structure inner surface of the shank of the body of rod of freezing bar and year bar extending sleeve.
Described freezing unit contains 1 load units, the external diameter >=3mm of extension 11.
Lengthening cover 3 is hollow tubular, upper end closed, lower ending opening, and on the described epimere sidewall lengthening cover and/or top is provided with ventilation 31.Freezing rod bush 2, in the reduced diameter at the position that the extension 11 with described freezing bar is socketed, lengthens cover 3 and is socketed on the position of described freezing rod bush 2 reduced diameter.
In one preferred embodiment, the body of rod of described freezing bar is hollow, is communicated with the inside of freezing rod bush 2 and lengthens the hollow structure of cover 1.
In one preferred embodiment, the bottom of described freezing rod bush 2 is provided with weight 21.Described weight is preferably metal material.
In one preferred embodiment, be provided with and overlap solid section between extension 11 and shank 12, the maximum outside diameter of the solid section of described cover equals the internal diameter of freezing rod bush opening part.The length of the solid section of described cover is preferably 0.1-10mm.One or two in the hollow structure inner surface of the solid section of described cover and lengthening cover is matsurface, to make to produce frictional force between the shank of the body of rod of freezing bar and the hollow structure inner surface carrying bar extending sleeve.The solid section of described cover can be the shank 12 of the body of rod of freezing bar or a part for extension 11.
Embodiment 2
The vitrified frozen vector system that the present embodiment provides, comprises freezing bar 1, freezing rod bush 2, lengthens cover 3 and protection cylinder 4.
Freezing bar 1 comprises the body of rod, the extension 11 of the described body of rod by bottom and the shank 12 on top, and the lower end of extension 11 is connected with frozen 13;
Freezing rod bush 2 is hollow structure, top closure, and upper end is provided with the opening be communicated with in external environment condition by described hollow structure; The opening of extension 11 through freezing rod bush 2 upper end of freezing bar is inserted in the hollow structure of freezing rod bush 2, forms load units.
The external diameter of extension 11 is less than the external diameter of shank 12, and the distance between the outer surface of extension 11 at shank 12 place and shank 12 outer surface equals the wall thickness of freezing bar loop bar 2 opening part.
The outer surface of the extension 11 of the body of rod of at least freezing bar and lengthen in the hollow structure inner surface of cover 2 one or two be matsurface, to make to produce frictional force between the hollow structure inner surface of the shank of the body of rod of freezing bar and year bar extending sleeve.
Described freezing unit contains one or more load units, and protection cylinder 4 is hollow structure, bottom end closure, upper end open, and the external diameter of described protection cylinder is 3-10mm, and the upper end open of protection cylinder is directly as window load units can put into and take out.
Lengthening cover 3 is hollow tubular, upper end closed, lower ending opening, and on the described epimere sidewall lengthening cover and/or top is provided with ventilation 31.Lengthen cover 3 lower end to reduce, be inserted in protection cylinder 4 from the top end opening of protection cylinder 4, the external diameter not reducing part of lengthening cover 3 is greater than the internal diameter of protection cylinder 4 top end opening, thus fixing lengthening is overlapped.
In one preferred embodiment, the bottom of described protection cylinder is provided with weight 41.Described weight 41 is preferably metal material.
In one preferred embodiment, the upper end of the body of rod of described freezing bar is provided with label and sampling section 5, label and sampling section 5 are the thin bar thinner than the body of rod or the flat-shaped body narrower than the body of rod, may be used for pasting label, be convenient to carry out mark identification, can also gripping be convenient to, such as, be inserted in fixing with the tubulose of pincet gripping or bottom end opening little bar cover 6 and take out.
Embodiment 3
The difference of the present embodiment and embodiment 2 is: protect in cylinder 4 and be provided with lateral partitions 42, protection cylinder is divided into multistage, each section forms protected location, wherein, the top end opening of protected location 401 topmost, lengthen cover 3 lower end to reduce, be inserted in protection cylinder 4 from the top end opening of protection cylinder 4, and sidewall is provided with the window 43 load units can put into and take out; The sidewall of other each protected locations 402 is provided with the window 43 of.
Be described in detail specific embodiments of the invention above, but it is as example, the present invention is not restricted to specific embodiment described above.To those skilled in the art, any equivalent modifications that this practicality is carried out and substituting also all among category of the present invention.Therefore, equalization conversion done without departing from the spirit and scope of the invention and amendment, all should contain within the scope of the invention.
Claims (16)
1. a vitrified frozen vector system, is characterized in that, comprises freezing unit, and described freezing unit comprises freezing bar and freezing rod bush, wherein,
Described freezing bar comprises the body of rod, and the described body of rod comprises the extension of bottom and the shank on top, and the lower end of described extension is connected with frozen, and described frozen is strip small pieces, and its cross section is circular arc spill;
Described freezing rod bush is hollow structure, and at least upper end is provided with the opening be communicated with external environment condition by described hollow structure; The opening of extension through freezing rod bush upper end of described freezing bar is inserted in the hollow structure of freezing rod bush, forms load units;
The external diameter of described extension is less than the external diameter of shank; Described freezing rod bush external diameter forms load units maximum outside diameter.
2. vitrified frozen vector system according to claim 1, is characterized in that, described extension is provided with shank junction and overlaps solid section, and the maximum outside diameter of the solid section of described cover equals the internal diameter of freezing rod bush opening part.
3. vitrified frozen vector system according to claim 2, is characterized in that, described freezing unit only contains 1 load units, the external diameter >=3mm of the extension of described freezing bar.
4. vitrified frozen vector system according to claim 3, is characterized in that, described freezing unit also comprises lengthening cover, and described lengthening cover is hollow structure at least partly, bottom end opening, and the hollow structure of described lengthening cover is communicated with the bottom end opening that lengthening is overlapped; The top of the shank of the body of rod of described freezing bar or be all inserted into from the bottom end opening lengthening cover lengthen cover hollow structure in.
5. vitrified frozen vector system according to claim 4, is characterized in that, the external diameter of the external diameter of the shank of described freezing bar or the lengthening cover that holds freezing bar shank is consistent with the load units maximum outside diameter that described freezing rod bush external diameter is formed.
6. vitrified frozen vector system according to claim 4, it is characterized in that, described freezing rod bush in the reduced diameter at the position that the extension with described freezing bar is socketed, but is greater than the external diameter of freezing bar shank, and described lengthening cover is socketed on the outer wall reduced of described freezing rod bush.
7. the vitrified frozen vector system according to claim 4 or 6, is characterized in that, the body of rod of described freezing bar is hollow, is communicated with the inside of freezing rod bush and lengthens the hollow structure of cover.
8. vitrified frozen vector system according to claim 2; it is characterized in that; described freezing unit contains one or more load units; described freezing unit also comprises bottom end closure and the protection cylinder of the hollow of upper end open; the external diameter of described protection cylinder is 3-10mm; the external diameter < 3mm of described freezing bar, described protection cylinder is provided with at least one window load units can put into and take out.
9. vitrified frozen vector system according to claim 8, is characterized in that, the distance of described freezing bar extension between the outer surface and handle outer surface at shank place equals the wall thickness of freezing bar loop bar opening part; The upper end of the body of rod of described freezing bar is provided with label and sampling section, and described label and sampling section are the thin bar thinner than the body of rod or the flat-shaped body narrower than the body of rod.
10. vitrified frozen vector system according to claim 9, it is characterized in that, the window of described protection cylinder is arranged on protection cylinder top, described freezing unit also comprises lengthening cover, described lengthening cover is hollow structure at least partly, bottom end opening, the hollow structure of described lengthening cover is communicated with the bottom end opening that lengthening is overlapped; Described lengthening cover lower end reduces, and be inserted in protection cylinder, lengthen the internal diameter that the external diameter not reducing part overlapped is greater than protection cylinder top end opening, thus fixing lengthening is overlapped from the top end opening of protection cylinder.
11. require the vitrified frozen vector system described in 10 according to claim, it is characterized in that, the length of described load units is less than to lengthen when cover is fixed on protection cylinder protects the bottom of cylinder to the length lengthening cover lower end.
12. require the vitrified frozen vector system described in 4 or 10 according to claim, it is characterized in that, described lengthening cover is hollow tubular, upper end closed, and on the described epimere sidewall lengthening cover and/or top is provided with ventilation hole.
13. require the vitrified frozen vector system described in 4 according to claim, it is characterized in that, are equipped with weight bottom described freezing rod bush.
14. require the vitrified frozen vector system described in 12 according to claim, it is characterized in that, are equipped with weight bottom described protection cylinder.
15. vitrified frozen vector systems according to claim 9, is characterized in that, the window of described protection cylinder is arranged on the side of protection cylinder, in straight strip or oblique strip.
16. vitrified frozen vector systems according to claim 15, is characterized in that, be provided with lateral partitions, protection cylinder is divided into multistage in described protection cylinder, and each section forms protected location, and the sidewall of each protected location is provided with the window of at least one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310596542.5A CN104642299A (en) | 2013-11-21 | 2013-11-21 | Vitrification freezing carrier system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310596542.5A CN104642299A (en) | 2013-11-21 | 2013-11-21 | Vitrification freezing carrier system |
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| Publication Number | Publication Date |
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| CN104642299A true CN104642299A (en) | 2015-05-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310596542.5A Pending CN104642299A (en) | 2013-11-21 | 2013-11-21 | Vitrification freezing carrier system |
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| CN (1) | CN104642299A (en) |
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| CN110637809A (en) * | 2019-10-10 | 2020-01-03 | 广州辅康生物科技有限公司 | Loading rod sleeve |
| CN115486437A (en) * | 2021-06-18 | 2022-12-20 | 上海明悦医疗科技有限公司 | Auxiliary reproduction carrier bar assembly |
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| CN115486437A (en) * | 2021-06-18 | 2022-12-20 | 上海明悦医疗科技有限公司 | Auxiliary reproduction carrier bar assembly |
| CN115486437B (en) * | 2021-06-18 | 2024-01-23 | 上海明悦医疗科技有限公司 | Auxiliary reproduction carrying rod assembly |
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Application publication date: 20150527 |
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