CN103749436B - Closed embryo cryopreservation carries bar and preparation method thereof and using method - Google Patents
Closed embryo cryopreservation carries bar and preparation method thereof and using method Download PDFInfo
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- CN103749436B CN103749436B CN201410029474.9A CN201410029474A CN103749436B CN 103749436 B CN103749436 B CN 103749436B CN 201410029474 A CN201410029474 A CN 201410029474A CN 103749436 B CN103749436 B CN 103749436B
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Abstract
A kind of closed embryo cryopreservation carries bar, comprise and carry bar body, carry bar body and be provided with upper and lower two-stage concave step, lower floor's concave step is fixed with the embryo slide of horizontal positioned, the middle part of embryo slide is provided with the groove placing embryo, and upper strata concave step is provided with to be connected to and carries on bar body and the cover plate matched with embryo slide.The present invention carries bar body, embryo slide and cover plate by establishing, and makes closed embryo's carrier, avoids the cross-infection in liquid nitrogen container; Carry bar body and adopt injection mo(u)lding, embryo slide adopts hot-forming, convenient for production, and cost of manufacture is low; Embryo slide and cover plate all adopt medical grade transparent PET thin slice, and transparency is good, and be easy to observe, and the surface tension after embryo slide sided corona treatment is set to 41-46 dyne, embryo is placed on it can not deform; Embryo being placed on after in embryo slide, by the method for pressing heat-sealing, cover plate being sealed carrying on bar body, easy to use.
Description
Technical field
The present invention relates to a kind of technical field of medicine equipment, particularly the closed embryo cryopreservation of one carries bar and preparation method thereof and using method.
Background technology
Along with the increase of infertile crowd, the people accepting embryo transplantation gets more and more, but the carrier of carrying embryo is simpler and cruder and coarse at present, melting post-tensioning flakiness, embryo being placed on it as burnt by glass bar; Or carry embryo with plastic tube, and last word plastic sheet is put embryo, then covers block, or the plastic bar of the direct arc that injection moulding one is thin places embryo.
The mode of carrying embryo recited above deposits defect in the following areas: first, initial carrier is up-to-date plastic slide, surface tension disunity, in use embryo can present spherical shape or pull into flat, thus be unfavorable for observing, be unfavorable for drawing the freezing liquid around embryo, if directly freezing like this, increasing thawing time when thawing, affecting embryo quality; The second, longer carrier does not have the point of fixed placement embryo, but be placed on certain position of slide glass according to doctor's custom, during observation, need to find embryo position, if embryo places too by end, can there is the risk touching down embryo in the dress sheath on slide glass; 3rd, the embryo of number people can be placed in a large liquid nitrogen bucket, and liquid nitrogen not single use, can there is the risk of viral cross-infection.
Summary of the invention
Technical problem to be solved by this invention is for the deficiencies in the prior art, provides a kind of reasonable in design, uses and preserves conveniently, pollution-free, ensures that the closed embryo cryopreservation of embryo quality carries bar.
Another technical problem to be solved by this invention is to provide a kind of closed embryo cryopreservation as previously mentioned and carries the preparation method of bar.
The present invention also can adopt the conventional technical method carrying bar of preparation to be prepared.
A technical problem more to be solved by this invention is to provide and a kind ofly adopts closed embryo cryopreservation described above to carry the using method that bar carries out embryo cryopreservation.
Technical problem to be solved by this invention is realized by following technical scheme, the present invention is that a kind of closed embryo cryopreservation carries bar, be characterized in, comprise and carry bar body, carry bar body and be provided with upper and lower two-stage concave step, lower floor's concave step is fixed with the embryo slide of horizontal positioned, the middle part of embryo slide is provided with the groove placing embryo, and upper strata concave step is provided with to be connected to and carries on bar body and the cover plate matched with embryo slide.
Technical problem to be solved by this invention can also be realized further by following technical scheme, carries in bar at above-described closed embryo cryopreservation: on year bar body, be also connected with handle.
Technical problem to be solved by this invention can also be realized further by following technical scheme, carries in bar at above-described closed embryo cryopreservation: the rounded setting of the groove in the middle part of described embryo slide.
Technical problem to be solved by this invention can also be realized further by following technical scheme, carries in bar at above-described closed embryo cryopreservation: the bottom land of described groove is flat.
Technical problem to be solved by this invention can also be realized further by following technical scheme, carries in bar at above-described closed embryo cryopreservation: the groove footpath of described groove is 0.5mm-2mm.
Technical problem to be solved by this invention can also be realized further by following technical scheme, carries in bar at above-described closed embryo cryopreservation: the thickness of described embryo slide is 0.075mm-0.12mm.
Technical problem to be solved by this invention can also be realized further by following technical scheme, carries in bar: the spatial volume formed between described cover plate and embryo slide is not more than 20mm at above-described closed embryo cryopreservation
3.
Another technical problem to be solved by this invention is realized by following technical scheme, the invention also discloses the preparation method that a kind of closed embryo cryopreservation described above carries bar, be characterized in, the method employing medical plastic integrated injection molding obtains year bar body being provided with upper and lower concave step; Medical grade transparent PET diaphragm is selected to make the material of embryo slide and cover plate, first sided corona treatment is carried out to embryo slide, the surface tension of embryo slide is made to reach 41-46 dyne, then adopt in the embryo slide of hot-press molding method after sided corona treatment and form groove, adopt the method for welding or hot melt embryo slide to be fixed in lower floor's concave step of year bar body again, adopt the method for welding to be fixed in the concave step of upper strata one end of cover plate.
Another technical problem to be solved by this invention is realized by following technical scheme, the invention also discloses and a kind ofly adopt closed embryo cryopreservation described above to carry the using method that bar carries out embryo cryopreservation, be characterized in, first embryo is placed on the groove of embryo slide, with push type heat sealing machine, cover plate pressing is sealed in the upper strata concave step of carrying bar body, embryo be enclosed in the groove of embryo slide, the embryo cryopreservation after closing carries bar and is placed in liquid nitrogen frozen tank and preserves.
Technical problem to be solved by this invention can also be realized further by following technical scheme, adopting closed embryo cryopreservation to carry bar carries out in the using method of embryo cryopreservation: during pressing heat-seal operation, the heat-sealing temperature of push type heat sealing machine is set to 200 °-290 °, compressing time is 2s-5s.
The present invention carries bar body, embryo slide and cover plate by establishing, and makes closed embryo's carrier, avoids the cross-infection in liquid nitrogen container.Embryo slide is established the groove placing embryo, time freezing, embryo is fixed in groove, prevents embryo's movement and deformation.Carry bar body and adopt injection mo(u)lding, embryo slide adopts hot-forming, and embryo slide and a year bar body are bonded by melt process, convenient for production, cost of manufacture is low.Embryo slide and cover plate all adopt medical grade transparent PET thin slice, and transparency is good, and be easy to observe, and the surface tension after embryo slide sided corona treatment is set to 41-46 dyne, embryo is placed on it can not deform.Embryo is placed on after in embryo slide, by the method for pressing heat-sealing, cover plate is sealed carrying on bar body, making embryo be enclosed in the groove of embryo slide, simple, convenient.Compared with prior art, the present invention is reasonable in design, and structure is simple, and production cost is low, easy and simple to handle, provides good Conservation environment to embryo.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is the partial schematic sectional view of carrying bar body in the present invention.
Embodiment
Referring to accompanying drawing, further describe concrete technical scheme of the present invention, so that those skilled in the art understands the present invention further, and do not form the restriction to its right.
Embodiment 1: see figures.1.and.2, a kind of closed embryo cryopreservation carries bar, comprise and carry bar body 1, carry bar body 1 and be provided with upper and lower two-stage concave step 2, lower floor's concave step 2 is fixed with the embryo slide 6 of horizontal positioned, the middle part of embryo slide 6 is provided with the groove 5 placing embryo, and upper strata concave step 2 is provided with to be connected to and carries on bar body 1 and the cover plate 3 matched with embryo slide 6.
Embodiment 2, described in embodiment 1, closed embryo cryopreservation carries in bar: at described year, bar body 1 is also connected with handle 4, conveniently fills in embryo's information.
Embodiment 3, described in embodiment 1 or 2, closed embryo cryopreservation carries in bar: the rounded setting of groove 5 in the middle part of described embryo slide 6.
Embodiment 4, embodiment 1-3 closed embryo cryopreservation described in any one carries in bar: the bottom land of described groove 5 is flat, conveniently places embryo, makes embryo not yielding.
Embodiment 5, embodiment 1-4 closed embryo cryopreservation described in any one carries in bar: the groove footpath of described groove 5 is 0.5mm.
Embodiment 6, embodiment 1-4 closed embryo cryopreservation described in any one carries in bar: the groove footpath of described groove 5 is 2mm.
Embodiment 7, embodiment 1-4 closed embryo cryopreservation described in any one carries in bar: the groove footpath of described groove 5 is 1.0mm.
Embodiment 8, embodiment 1-7 closed embryo cryopreservation described in any one carries in bar: the thickness of described embryo slide 6 is 0.075mm.
Embodiment 9, embodiment 1-7 closed embryo cryopreservation described in any one carries in bar: the thickness of described embryo slide 6 is 0.12mm.
Embodiment 10, embodiment 1-7 closed embryo cryopreservation described in any one carries in bar: the thickness of described embryo slide 6 is 0.1mm.
Can, under the prerequisite ensureing preferably freezing rate, effectively prevent embryo slide from breaking by preferred preferably embryo slide thickness.
Embodiment 11, embodiment 1-10 closed embryo cryopreservation described in any one carries in bar: the spatial volume formed between described cover plate 3 and embryo slide 6 is not more than 20mm
3.
Embodiment 12, embodiment 1-10 closed embryo cryopreservation described in any one carries in bar: the spatial volume formed between described cover plate 3 and embryo slide 6 is 20mm
3;
Embodiment 13, embodiment 1-10 closed embryo cryopreservation described in any one carries in bar: the spatial volume formed between described cover plate 3 and embryo slide 6 is 15mm
3.
Embodiment 14: a kind ofly carry the preparation method of bar as the closed embryo cryopreservation of embodiment 1-13 as described in any one, what the method adopted the method for medical plastic integrated injection molding to obtain to be provided with upper and lower concave step 2 carries bar body 1; Medical grade transparent PET diaphragm is selected to make the material of embryo slide 6 and cover plate 3, first sided corona treatment is carried out to embryo slide 6, the surface tension of embryo slide 6 is made to reach 41 dyne, then adopt in the embryo slide 6 of hot-press molding method after sided corona treatment and form groove 5, adopt the method for welding or hot melt embryo slide 6 to be fixed in lower floor's concave step 2 of year bar body 1 again, adopt the method for welding to be fixed in upper strata concave step 2 one end of cover plate 3.
Embryo slide 6 described in the present embodiment and cover plate 3 adopt medical grade transparent PET thin slice to make, its stable performance, can not brittle failure or the transparency change at-196 DEG C, embryo slide 6 surface in this device will through sided corona treatment, embryo is placed on it can not glomeration, also can not present too flat.
Embodiment 15, in the preparation method described in embodiment 14: the surface tension after embryo slide 6 carries out sided corona treatment reaches 46 dyne, all the other are all identical with embodiment 14.
Embodiment 16, in the preparation method described in embodiment 14: the surface tension after embryo slide 6 carries out sided corona treatment reaches 43 dyne, all the other are all identical with embodiment 14.
Embodiment 17: a kind of employing such as embodiment 1-13 closed embryo cryopreservation as described in any one carries the using method that bar carries out embryo cryopreservation, first embryo is placed on the groove 5 of embryo slide 6, with push type heat sealing machine, cover plate pressing is sealed in the upper strata concave step 2 of carrying bar body 1, embryo be enclosed in the groove 5 of embryo slide 6, the embryo cryopreservation after closing carries bar and is placed in liquid nitrogen frozen tank and preserves.
Embodiment 18, in the using method described in embodiment 17: during pressing heat-seal operation, the heat-sealing temperature of push type heat sealing machine is set to 200 °, compressing time is 5s.
Embodiment 19, in the using method described in embodiment 17: during pressing heat-seal operation, the heat-sealing temperature of push type heat sealing machine is set to 290 °, compressing time is 2s.
Embodiment 20, in the using method described in embodiment 17: during pressing heat-seal operation, the heat-sealing temperature of push type heat sealing machine is set to 250 °, compressing time is 3s.
Claims (8)
1. a closed embryo cryopreservation carries the using method of bar, it is characterized in that: described closed embryo cryopreservation carries bar and comprises a year bar body, carry bar body and be provided with upper and lower two-stage concave step, lower floor's concave step is fixed with the embryo slide of horizontal positioned, the middle part of embryo slide is provided with the groove placing embryo, and upper strata concave step is provided with to be connected to and carries on bar body and the cover plate matched with embryo slide;
First embryo is placed on the groove of embryo slide, with push type heat sealing machine, cover plate pressing is sealed in the upper strata concave step of carrying bar body, the heat-sealing temperature of push type heat sealing machine is set to 200 °-290 °, compressing time is 2s-5s, embryo be enclosed in the groove of embryo slide, the embryo cryopreservation after closing carries bar and is placed in liquid nitrogen frozen tank and preserves.
2. closed embryo cryopreservation according to claim 1 carries the using method of bar, it is characterized in that: at described year, bar body is also connected with handle.
3. closed embryo cryopreservation according to claim 1 carries the using method of bar, it is characterized in that: the rounded setting of the groove in the middle part of described embryo slide.
4. the closed embryo cryopreservation according to claim 1 or 3 carries the using method of bar, it is characterized in that: the bottom land of described groove is flat.
5. the closed embryo cryopreservation according to claim 1 or 3 carries the using method of bar, it is characterized in that: the groove footpath of described groove is 0.5mm-2mm.
6. closed embryo cryopreservation according to claim 1 carries the using method of bar, it is characterized in that: the thickness of described embryo slide is 0.075mm-0.12mm.
7. closed embryo cryopreservation according to claim 1 carries the using method of bar, it is characterized in that: the spatial volume formed between described cover plate and embryo slide is not more than 20mm
3.
8. closed embryo cryopreservation according to claim 1 carries the using method of bar, it is characterized in that: the preparation method that described closed embryo cryopreservation carries bar is as follows, and employing medical plastic integrated injection molding obtains year bar body being provided with upper and lower concave step; Medical grade transparent PET diaphragm is selected to make the material of embryo slide and cover plate, first sided corona treatment is carried out to embryo slide, the surface tension of embryo slide is made to reach 41-46 dyne, then adopt in the embryo slide of hot-press molding method after sided corona treatment and form groove, adopt the method for welding or hot melt embryo slide to be fixed in lower floor's concave step of year bar body again, adopt the method for welding to be fixed in the concave step of upper strata one end of cover plate.
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| CN201410029474.9A CN103749436B (en) | 2014-01-23 | 2014-01-23 | Closed embryo cryopreservation carries bar and preparation method thereof and using method |
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| CN103749436B true CN103749436B (en) | 2016-04-13 |
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| CN110320080B (en) * | 2019-07-16 | 2022-01-25 | 宁夏医科大学总医院 | Antigen high-pressure steaming heat repair device and repair method |
| CN113632785B (en) * | 2021-08-17 | 2024-04-05 | 武汉互创联合科技有限公司 | Freezing carrying rod and operation method thereof |
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| CN102405901A (en) * | 2010-09-21 | 2012-04-11 | 北京微创介入医疗装备有限公司 | Suction type glass vitrification freezing carrier device |
| CN202311020U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | Embryo vitrification freezing carrier |
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| CN203340879U (en) * | 2013-06-19 | 2013-12-18 | 深圳华大基因科技有限公司 | Bearing tool for vitrified cryopreservation of biological material |
| CN203692297U (en) * | 2014-01-23 | 2014-07-09 | 江苏苏云医疗器材有限公司 | Closed embryo freezing carrying rod |
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2014
- 2014-01-23 CN CN201410029474.9A patent/CN103749436B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102405901A (en) * | 2010-09-21 | 2012-04-11 | 北京微创介入医疗装备有限公司 | Suction type glass vitrification freezing carrier device |
| CN202311020U (en) * | 2011-12-05 | 2012-07-11 | 戴志俊 | Embryo vitrification freezing carrier |
| CN203152372U (en) * | 2013-04-16 | 2013-08-28 | 曾兴光 | Embryo freezing carrier device |
| CN203340879U (en) * | 2013-06-19 | 2013-12-18 | 深圳华大基因科技有限公司 | Bearing tool for vitrified cryopreservation of biological material |
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