CN218898090U - Hematopoietic stem cell cryopreservation bag - Google Patents

Abstract

The utility model relates to the technical field of medical freezing bags, in particular to a hematopoietic stem cell freezing bag which comprises a freezing bag main body, a first liquid adding pipe, a second liquid adding pipe and a third liquid adding pipe, wherein one end of the first liquid adding pipe is connected to the upper end of the freezing bag main body, one end of the second liquid adding pipe is connected to the lower end of the freezing bag main body, the other end of the first liquid adding pipe and the other end of the second liquid adding pipe are connected to one end of the third liquid adding pipe, a distribution pipe is arranged on the inner side of the sealed edge of the freezing bag main body, and a plurality of distribution holes are uniformly formed in the distribution pipe. The freezing protection agent is infused simultaneously to the two ends of the inner side of the freezing storage bag main body through the first liquid adding pipe and the second liquid adding pipe, and the freezing protection agent is uniformly distributed through the distribution pipe and the distribution holes, so that the uniform distribution of the freezing protection agent can be effectively promoted, the problem that the local concentration of the freezing protection agent in the freezing storage bag main body is too high is prevented, the infusion speed of the freezing protection agent is improved, and the toxic damage to cells is reduced.

Description

Hematopoietic stem cell cryopreservation bag

Technical Field

The utility model relates to the technical field of medical cryopreservation bags, in particular to a hematopoietic stem cell cryopreservation bag.

Background

In the process of freezing and preserving the hematopoietic stem cells, a cell freezing protective agent is needed, but the cell freezing protective agent always has certain cytotoxicity, so that the activity of the hematopoietic stem cells is reduced due to overlong infusion time or overhigh local freezing protective agent concentration in the process of infusing the cell freezing protective agent. In the prior art, the freezing bag used in the laboratory is mainly provided with only one liquid adding pipe, so that the infusion process of the cryoprotectant is greatly prolonged in order to prevent the concentration of the local cryoprotectant from being too high, the infusion time is half of the infusion process, and the cytoblast is damaged by toxicity due to overlong contact time between the cryoprotectant and the cryoprotectant.

Disclosure of Invention

The utility model provides a hematopoietic stem cell cryopreservation bag, which aims to solve the problems that the prior cryopreservation bag has a simple structure, and local concentration is easy to be too high or the infusion time is too long when a cryoprotectant is infused, so that cells are damaged.

The technical scheme adopted for solving the technical problems is as follows: the utility model provides a hematopoietic stem cell freezes deposits bag, includes freezes deposits bag main part, first liquid feeding pipe, second liquid feeding pipe and third liquid feeding pipe, first liquid feeding pipe one end is connected in freezes deposits bag main part upper end, and second liquid feeding pipe one end is connected in freezes deposits bag main part lower extreme, and first liquid feeding pipe other end, second liquid feeding pipe other end are connected in third liquid feeding pipe one end, the airtight edge inboard of freezes deposits bag main part is equipped with the distribution pipe, evenly is equipped with a plurality of distribution hole on the distribution pipe.

As optimization, the upper end of the freezing bag main body is provided with a sampling tube, the outer end of the sampling tube is provided with a knob, the inside of the sampling tube is provided with an inner protection sleeve, and the outside of the sampling tube is provided with an outer protection sleeve.

As optimization, be connected with the tee bend between first liquid feeding pipe, second liquid feeding pipe and the third liquid feeding pipe, be equipped with on the pipeline that first liquid feeding pipe and tee bend are adjacent and draw in the section, draw in the section diameter and be less than the diameter of first liquid feeding pipe, be equipped with the filling opening on drawing in the section and freezing the first liquid feeding pipe between the bag main part, the filling opening outer end is equipped with first cap that revolves.

As optimization, the second liquid adding pipe and the third liquid adding pipe are both provided with regulators, and the third liquid adding pipe is provided with a second rotary cap.

As optimization, the one end that second liquid feeding pipe links to each other with the cryopreservation bag main part is connected with the distribution pipe middle part link up, and the distribution pipe upwards extends to the cryopreservation bag main part top along the bottom of cryopreservation bag main part.

As optimization, the distribution holes are arranged towards the middle of the freezing storage bag main body, and the distribution pipes are arranged along the inner side edge of the freezing storage bag main body in a fitting way.

The beneficial effect of this scheme is: a hematopoietic stem cell cryopreservation bag having the following benefits:

the first liquid adding pipe, the second liquid adding pipe and the third liquid adding pipe are connected to the freezing storage bag main body, the freezing protective agent is infused into the two ends of the inner side of the freezing storage bag main body through the first liquid adding pipe and the second liquid adding pipe at the same time, and the freezing protective agent is uniformly distributed through the distribution pipes and the distribution holes, so that the uniform distribution of the freezing protective agent can be effectively promoted, the problem of overhigh local concentration of the freezing protective agent in the freezing storage bag main body is prevented, the infusion speed of the freezing protective agent is improved, and the toxic damage to cells is reduced;

the inner protective sleeve is arranged in the sampling port of the freezing bag main body, the outer protective sleeve is arranged outside the sampling port, so that the external pollution caused by thawing and resuscitating of the freezing bag main body can be effectively prevented, the loss is reduced, and the liquid flow rates inside the two liquid adding pipes can be balanced due to the arrangement of the collecting section of the first liquid adding pipe;

the distribution pipe is arranged along the inner side edge of the freezing bag main body in a fitting way, so that the phenomenon of uneven melting of umbilical blood in the freezing bag caused by the distribution pipe in the resuscitation process is reduced.

Drawings

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is a schematic structural view of the present utility model omitting the outer protective sheath.

Wherein, 1, the main body of the freezing bag, 2, a first liquid adding pipe, 3, a second liquid adding pipe, 4, a third liquid adding pipe, 5, a sampling pipe, 6, a knob, 7 and an inner layer protecting sleeve, 8, an outer layer protective sleeve, 9, a tee joint, 10, a furling section, 11, a liquid adding port, 12, a first rotary cap, 13, a regulator, 14, a second rotary cap, 15, a distribution pipe, 16 and distribution holes.

Detailed Description

For the purpose of making the objects, technical solutions and advantages of the embodiments of the present utility model more apparent, the technical solutions of the embodiments of the present utility model will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present utility model, and it is apparent that the described embodiments are some embodiments of the present utility model, but not all embodiments of the present utility model. The components of the embodiments of the present utility model generally described and illustrated in the figures herein may be arranged and designed in a wide variety of different configurations. Thus, the following detailed description of the embodiments of the utility model, as presented in the figures, is not intended to limit the scope of the utility model, as claimed, but is merely representative of selected embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.

In the description of the present utility model, it should be noted that, directions or positional relationships indicated by terms such as "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc., are directions or positional relationships based on those shown in the drawings, or are directions or positional relationships conventionally put in use of the inventive product, are merely for convenience of describing the present utility model and simplifying the description, and are not indicative or implying that the apparatus or element to be referred to must have a specific direction, be constructed and operated in a specific direction, and thus should not be construed as limiting the present utility model. Furthermore, the terms "first," "second," "third," and the like are used merely to distinguish between descriptions and should not be construed as indicating or implying relative importance.

As shown in fig. 1, a hematopoietic stem cell cryopreservation bag comprises a cryopreservation bag body 1, a first liquid adding pipe 2, a second liquid adding pipe 3 and a third liquid adding pipe 4, wherein one end of the first liquid adding pipe 2 is connected to the upper end of the cryopreservation bag body 1, one end of the second liquid adding pipe 3 is connected to the lower end of the cryopreservation bag body 1, the other end of the first liquid adding pipe 2 and the other end of the second liquid adding pipe 3 are connected to one end of the third liquid adding pipe 4, a distribution pipe 15 is arranged on the inner side of the sealed edge of the cryopreservation bag body 1, and a plurality of distribution holes 16 are uniformly formed in the distribution pipe 15.

As shown in fig. 1 and 2, the upper end of the freezing bag main body 1 is provided with a sampling tube 5, the outer end of the sampling tube 5 is provided with a knob 6, the inside of the sampling tube 5 is provided with an inner protection sleeve 7, and the outside of the sampling tube 5 is provided with an outer protection sleeve 8.

As shown in fig. 1, a tee joint 9 is connected between the first liquid adding pipe 2, the second liquid adding pipe 3 and the third liquid adding pipe 4, a furling section 10 is arranged on a pipeline adjacent to the tee joint 9 of the first liquid adding pipe 2, the diameter of the furling section 10 is smaller than that of the first liquid adding pipe 2, a liquid adding opening 11 is arranged on the first liquid adding pipe 2 between the furling section 10 and the freezing storage bag main body 1, and a first rotary cap 12 is arranged at the outer end of the liquid adding opening 11.

As shown in fig. 1, the second liquid adding pipe 3 and the third liquid adding pipe 4 are respectively provided with a regulator 13, and the third liquid adding pipe 4 is provided with a second rotary cap 14.

As shown in fig. 1, one end of the second liquid adding tube 3 connected with the freezing bag main body 1 is connected with the middle part of the distribution tube 15 in a penetrating way, and the distribution tube 15 extends upwards to the top of the freezing bag main body 1 along the bottom of the freezing bag main body 1.

As shown in fig. 1, the distribution holes 16 are disposed toward the middle of the freezing bag main body 1, and the distribution pipes 15 are disposed along the inner edge of the freezing bag main body 1.

The device has the following steps when being specifically used;

the two regulators 13 are closed, then the first screw cap 12 is opened, and the separated hematopoietic stem cells are added into the freezing bag main body 1 through the liquid adding opening 11 and the first liquid adding pipe 2;

according to the weight of cord blood in the freezing bag main body 1, a certain amount of freezing protecting agent is extracted by using a syringe, the second rotary cap 14 is opened, and the syringe with the freezing protecting agent is connected to the third liquid adding pipe 3;

precooling the whole device to 4 ℃, opening two regulators 13, installing the other end of the injector on an infusion pump, placing the freezing bag main body 1 on a shaking table to shake, and starting to execute infusion and mixing procedures;

after the uniform mixing is completed, the air in the injector is utilized to push all the liquid in the liquid adding pipe into the freezing and storing bag main body 1, the regulator 13 is closed, and then the second liquid adding pipe 3 is thermally sealed from one end close to the freezing and storing bag main body 1 and the tee joint 9;

the air exhaust and sample reserving are carried out through the liquid adding opening 11 of the first liquid adding pipe 2, and after the operation is finished, one end of the first liquid adding pipe 2, which is close to the freezing bag main body 1, is subjected to heat sealing and sample reserving.

In the resuscitation process, the freezing bag main body 1 is placed in a 37 ℃ water bath to be melted, the surface moisture of the freezing bag main body 1 is wiped to be dried after the melting, and the outer protective sleeve 8 at the sampling tube 5 is opened, the knob 6 is screwed off, and the inner protective sleeve 7 is punctured to take blood.

The above embodiments are merely specific examples of the present utility model, and the scope of the present utility model includes, but is not limited to, the product forms and styles of the above embodiments, any suitable changes or modifications of the hematopoietic stem cell cryopreservation bag according to the claims of the present utility model by those skilled in the art shall fall within the scope of the present utility model.

Claims (6)

1. A hematopoietic stem cell cryopreservation bag, which is characterized in that: including freezing and depositing bag main part, first liquid feeding pipe, second liquid feeding pipe and third liquid feeding pipe, first liquid feeding pipe one end is connected in freezing and depositing bag main part upper end, and second liquid feeding pipe one end is connected in freezing and depositing bag main part lower extreme, and first liquid feeding pipe other end, second liquid feeding pipe other end are connected in third liquid feeding pipe one end, freezing airtight edge inboard of depositing the bag main part is equipped with the distribution pipe, evenly is equipped with a plurality of distribution hole on the distribution pipe.

2. The hematopoietic stem cell cryopreservation bag of claim 1, wherein: the freezing bag is characterized in that a sampling tube is arranged at the upper end of the freezing bag main body, a knob is arranged at the outer end of the sampling tube, an inner-layer protection sleeve is arranged inside the sampling tube, and an outer-layer protection sleeve is arranged outside the sampling tube.

3. The hematopoietic stem cell cryopreservation bag of claim 2, wherein: the three-way freezing storage bag is characterized in that a tee joint is connected among the first liquid adding pipe, the second liquid adding pipe and the third liquid adding pipe, a furling section is arranged on a pipeline adjacent to the tee joint, the diameter of the furling section is smaller than that of the first liquid adding pipe, a liquid adding opening is arranged on the first liquid adding pipe between the furling section and the freezing storage bag main body, and a first rotary cap is arranged at the outer end of the liquid adding opening.

4. A hematopoietic stem cell cryopreservation bag according to claim 3, wherein: the second liquid adding pipe and the third liquid adding pipe are respectively provided with a regulator, and the third liquid adding pipe is provided with a second rotary cap.

5. The hematopoietic stem cell cryopreservation bag of claim 4, wherein: one end of the second liquid adding pipe connected with the freezing storage bag main body is connected with the middle part of the distribution pipe in a penetrating way, and the distribution pipe extends upwards to the top of the freezing storage bag main body along the bottom of the freezing storage bag main body.

6. The hematopoietic stem cell cryopreservation bag of claim 5, wherein: the distribution holes are arranged towards the middle of the freezing storage bag main body, and the distribution pipes are arranged along the inner side edge of the freezing storage bag main body in a fitting mode.

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