CN205624138U - Cell cryopreserving frame - Google Patents
Cell cryopreserving frame Download PDFInfo
- Publication number
- CN205624138U CN205624138U CN201620341922.3U CN201620341922U CN205624138U CN 205624138 U CN205624138 U CN 205624138U CN 201620341922 U CN201620341922 U CN 201620341922U CN 205624138 U CN205624138 U CN 205624138U
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- CN
- China
- Prior art keywords
- frame
- cell cryopreservation
- tube seat
- cell
- tube
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Abstract
The utility model relates to a cell cryopreserving device specifically provides a cell cryopreserving frame. Cell cryopreserving frame is solid support body, embedded be used for placing freeze the tube seat of depositing the pipe, the tube seat bottom is equipped with deposits the block structure that the socle bilge construction corresponds and is used for fixing to freeze depositing the pipe with freezing, the material of solid support body is the metal, like stainless steel, aluminum alloy, titanium alloy, copper alloy, copper, aluminium etc. Deposit the pipe when placing at the tube seat, because its polycrystalline substance corresponds with the block structure when freezing for freeze when depositing tub in non - vertical direction operation, can guarantee to freeze the stability of depositing the pipe, very big degree top to freezing the one -hand operation who deposits the pipe. During actual operation, put into the precooling with cell cryopreserving pipe after among the cell cryopreserving frame to put into the foam insulation can with the support body, can directly put into 80 in the refrigerator, the process of realization procedure cooling. Simultaneously, need not add chemical reagent such as isopropyl alcohol, safe and pollution -free. Operation process is simple and convenient, and low cost is energy -concerving and environment -protective.
Description
Technical field
This utility model relates to cell cryopreservation device, specifically, relates to cell cryopreservation frame.
Background technology
Cell cryopreservation is one of main method of cell preservation, utilizes Cryopreservation Technology to be placed in by cell
Cryopreservation in-196 DEG C of liquid nitrogen, can make cell be temporarily disengaged from growth conditions and its cell is special
Property saves, and plays the effect of cell conservation.Freeze-stored cell generally has two kinds of methods: tradition
Methods and procedures is lowered the temperature.Traditional method is that cryovial is first placed on 4 DEG C ,-20 DEG C ,-80 DEG C and
In liquid nitrogen, the method complex operation, frozen weak effect；Programmed cooling is to utilize program temperature reduction box
Setting program is down to-80 DEG C by room temperature, transfers in liquid nitrogen, and the method is frozen effective,
But program temperature reduction box is expensive, and need addition isopropanol to realize programmed cooling, pollute sky
Gas.At present, it is easy and simple to handle that technical field of cell biology does not the most also have one to be capable of, and becomes
This is cheap, the cell cryopreservation device of energy-conserving and environment-protective.
Utility model content
(1) to solve the technical problem that
The purpose of this utility model is to provide a kind of cell cryopreservation frame, solves to carry out in prior art
During programmed cooling, program temperature reduction box needs to add the pollution problem that isopropanol causes.
(2) technical scheme
In order to solve above-mentioned technical problem, this utility model provides a kind of cell cryopreservation frame.
Described cell cryopreservation frame is solid support body, the embedded tube seat for placing cryopreservation tube, tube seat
Bottom is provided with the snap-in structure corresponding with cryopreservation tube polycrystalline substance for fixing cryopreservation tube.
When cryopreservation tube is placed on tube seat, owing to its polycrystalline substance is corresponding with snap-in structure so that
When non-vertical direction operation cryopreservation tube, it is possible to ensure stablizing, above high degree of cryopreservation tube
One-handed performance to cryopreservation tube.
The material of described solid support body is metal, for the ease of cleaning and preserving, the most stainless
Metal, such as rustless steel, aluminium alloy, titanium alloy, copper alloy, copper, aluminum etc..
In a particular embodiment of the present invention, the material of described solid support body is preferably aluminium alloy.
Described tube seat can be one or more, and in order to improve application efficiency, preferably tube seat is multiple.
The specification of tube seat can be adjusted according to the specification of cryopreservation tube, to realize accommodating cryopreservation tube
Purpose.
In order to the cryopreservation tube of 0.5-2.0ml specification frozen is better achieved, this utility model provides
A kind of cell cryopreservation frame being specifically designed for 0.5-2.0ml specification cryopreservation tube.In described cell cryopreservation frame
Embedding multiple tube seat, is uniformly distributed, and the center of circle distance of adjacent tube seat is 15～20mm, at this tube seat
Density of setting under, it is ensured that place cryopreservation tube therein and realize cooling fast and effectively and freeze
Deposit.The a diameter of 12.5mm of notch of described tube seat, the height of tube seat is that 35-39mm (includes
Snap-in structure), wherein, snap-in structure is positioned at tube seat bottom, 8-9mm.
Snap-in structure is divided into upper and lower: top is cylinder, diameter 8mm, high 6mm,
Bottom is cone, high 2-3mm；Wherein, the inwall of cylindrical upper section is provided with impressing, described
Impressing is uniformly distributed, totally 6, and depth of indentation is 1.5mm.
Further, in order to improve the utilization rate of material, cell cryopreservation tube is farthest realized
Frozen.The specification of described cell cryopreservation frame is long 125mm* width 40mm* height 46mm.Described
Tube seat totally two row, often row 6, totally 12, the center of circle distance of adjacent tube seat is 19mm.
Further, the movement of the most described cell cryopreservation frame, described cell cryopreservation frame
Also can be provided with handle structure, described handle structure can be the appropriateness extension of support body top surface edge,
It is alternatively other and is suitable to portable structure.
Such as, support body specification as above time, the length appropriateness of support body upper surface can be increased to
143mm, it is simple to operator take.Also other are easy to behaviour can to arrange handle etc. outside support body
The structure taken as personnel.
During application, in advance cell cryopreservation frame is positioned over pre-cooling in 4 DEG C of refrigerators, time >=30min；
Cell cryopreservation tube used is positioned in the cell cryopreservation frame after pre-cooling；Cells frozen storing liquid will be used
The complete cell suspension of suspendible is quickly positioned in cell cryopreservation tube；Cell cryopreservation frame is used Cotton Gossypii
Parcel, and be positioned in foam thermal insulation box；Foam thermal insulation box is positioned in-80 DEG C of refrigerators；
After 24h, cell cryopreservation tube is transferred quickly in liquid nitrogen,.
(3) beneficial effect
The cell cryopreservation frame that this utility model provides, uses special substance so that by cell cryopreservation
Pipe is put in the cell cryopreservation frame after pre-cooling, and is put into by support body in foam insulation box, can be direct
Put in-80 refrigerators, it is achieved the process of programmed cooling.Simultaneously, it is not necessary to add isopropanol etc.
Chemical reagent, safety non-pollution.Operating process is easy, with low cost, energy-conserving and environment-protective.
Accompanying drawing explanation
Fig. 1 is cell cryopreservation frame side view described in the utility model；
Fig. 2 is the top view of cell cryopreservation frame described in the utility model；
In figure, 1: support body；2: tube seat；3: snap-in structure.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, in this utility model embodiment
Technical scheme be clearly and completely described, it is clear that described embodiment be only this
The a part of embodiment of utility model rather than whole embodiments.Based in this utility model
Embodiment, those of ordinary skill in the art are obtained on the premise of not making creative work
Every other embodiment, broadly fall into this utility model protection scope.
In description of the present utility model, it should be noted that term " " center ", " longitudinally ",
" laterally ", " on ", D score, "front", "rear", "left", "right", " vertically ", " level ",
" push up ", " end " " interior ", the orientation of the instruction such as " outward " or position relationship be for based on shown in accompanying drawing
Orientation or position relationship, be for only for ease of description the present invention and simplify describe rather than refer to
Show or imply that the device of indication or element must have specific orientation, with specific azimuth configuration
And operation, therefore it is not intended that to restriction of the present utility model.
Embodiment 1
Present embodiments provide a kind of cell cryopreservation frame, as it is shown in figure 1, described cell cryopreservation frame
For solid support body, the embedded tube seat for placing cryopreservation tube, be provided with bottom tube seat with cryopreservation tube at the bottom of
Snap-in structure corresponding to portion's structure is used for fixing cryopreservation tube；The material of described solid support body is that aluminum closes
Gold.
The specification of described cell cryopreservation frame is long 125mm* width 40mm* height 46mm, described tube seat
Totally two row, often row 6.
As in figure 2 it is shown, embedded 12 tube seats of described cell cryopreservation frame, it is uniformly distributed, adjacent tubes
The center of circle distance of groove is 19mm, a diameter of 12.5mm of notch of described tube seat, the height of tube seat
For 39mm；Snap-in structure is positioned at tube seat bottom, altogether 8mm, including: cylindrical upper section is (straight
Footpath 8mm, high 6mm) and conical lower (high 2mm).
The inwall of cylindrical upper section is provided with equally distributed 6 impressings, and depth of indentation is 1.5mm.
Embodiment 2
The present embodiment is with the difference of embodiment 1, and described cell cryopreservation frame is additionally provided with portable knot
Structure, described handle structure is stretched out by support body top surface edge and is formed, and is specially on support body
The length appropriateness on surface increases to 143mm, it is simple to operator take.
Embodiment 3
The present embodiment is with the difference of embodiment 1, is replaced with by the material of described solid support body
Rustless steel.
The above, detailed description of the invention the most of the present utility model, but guarantor of the present utility model
The scope of protecting is not limited thereto, and any those familiar with the art is at this utility model
In the technical scope disclosed, the change that can readily occur in or replacement, all should contain in this practicality new
Within the protection domain of type.Therefore, protection domain of the present utility model should be with described claim
Protection domain be as the criterion.
Claims (10)
1. a cell cryopreservation frame, it is characterised in that described cell cryopreservation frame is solid support body,
The embedded tube seat for placing cryopreservation tube, is provided with corresponding with cryopreservation tube polycrystalline substance bottom tube seat
Snap-in structure is used for fixing cryopreservation tube；The material of described solid support body is metal.
Cell cryopreservation frame the most according to claim 1, it is characterised in that described solid frame
The material of body is aluminium alloy.
Cell cryopreservation frame the most according to claim 1 and 2, it is characterised in that described pipe
Groove can be multiple.
Cell cryopreservation frame the most according to claim 3, it is characterised in that described cell freezes
Depositing the embedded multiple tube seats of frame, be uniformly distributed, the center of circle distance of adjacent tube seat is 15～20mm, institute
Stating a diameter of 12.5mm of notch of tube seat, the height of tube seat is 35-39mm；Snap-in structure position
In tube seat bottom, 8-9mm.
Cell cryopreservation frame the most according to claim 4, it is characterised in that described engaging is tied
Structure is divided into upper and lower: top is cylinder, and diameter 8mm, high 6mm, bottom is circular cone
Shape, high 2-3mm.
Cell cryopreservation frame the most according to claim 5, it is characterised in that cylindrical upper section
Inwall be provided with impressing.
Cell cryopreservation frame the most according to claim 6, it is characterised in that described impressing is equal
Even distribution, totally 6, depth of indentation is 1.5mm.
8. according to the cell cryopreservation frame described in any one of claim 4-7, it is characterised in that institute
The specification stating cell cryopreservation frame is long 125mm* width 40mm* height 46mm, described tube seat totally two
Row, often row 6.
9. according to the cell cryopreservation frame described in claim 1-2 or any one of 4-7, it is characterised in that
Described cell cryopreservation frame is additionally provided with handle structure.
Cell cryopreservation frame the most according to claim 9, it is characterised in that described portable
Structure is stretched out by support body top surface edge and is formed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620341922.3U CN205624138U (en) | 2016-04-21 | 2016-04-21 | Cell cryopreserving frame |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620341922.3U CN205624138U (en) | 2016-04-21 | 2016-04-21 | Cell cryopreserving frame |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205624138U true CN205624138U (en) | 2016-10-12 |
Family
ID=57062325
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620341922.3U Expired - Fee Related CN205624138U (en) | 2016-04-21 | 2016-04-21 | Cell cryopreserving frame |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205624138U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11311008B2 (en) | 2016-04-19 | 2022-04-26 | Regents Of The University Of Minnesota. | Cryopreservation compositions and methods involving nanowarming |
-
2016
- 2016-04-21 CN CN201620341922.3U patent/CN205624138U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11311008B2 (en) | 2016-04-19 | 2022-04-26 | Regents Of The University Of Minnesota. | Cryopreservation compositions and methods involving nanowarming |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20161012 Termination date: 20210421 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |