CN205071975U - Pipe is deposited in freezing of unfreezeing fast - Google Patents
Pipe is deposited in freezing of unfreezeing fast Download PDFInfo
- Publication number
- CN205071975U CN205071975U CN201520752653.5U CN201520752653U CN205071975U CN 205071975 U CN205071975 U CN 205071975U CN 201520752653 U CN201520752653 U CN 201520752653U CN 205071975 U CN205071975 U CN 205071975U
- Authority
- CN
- China
- Prior art keywords
- cryopreservation tube
- lid
- seal cover
- freezing
- sealed lid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000007710 freezing Methods 0.000 title abstract description 15
- 230000008014 freezing Effects 0.000 title abstract description 15
- 238000005138 cryopreservation Methods 0.000 claims description 45
- 238000001125 extrusion Methods 0.000 claims description 25
- 238000010257 thawing Methods 0.000 claims description 19
- 210000003462 vein Anatomy 0.000 claims description 9
- 239000003550 marker Substances 0.000 claims description 3
- 238000011084 recovery Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 abstract 2
- 238000000151 deposition Methods 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 239000001963 growth medium Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 22
- 239000000203 mixture Substances 0.000 description 11
- 238000000034 method Methods 0.000 description 6
- 238000007789 sealing Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- 230000000975 bioactive effect Effects 0.000 description 2
- 238000005842 biochemical reaction Methods 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000007634 remodeling Methods 0.000 description 1
- 238000001356 surgical procedure Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a pipe is deposited in freezing of unfreezeing fast, include: freeze and deposit the pipe, sealed lid, the tensile body, label differentiation stopper, tensile body upper end fix the top activity inslot in sealed lid, the lower extreme deepening freeze deposit manage in the middle of, sealed lid is freezing soon and is depositing a tub upper end, the label distinguish that the stopper is located the recess of the outer upper surface of sealed lid in the middle of, tensile body upper end be rotator, middle for tensile strip, the lower part be spheroid, comes back to life or consciousness when unfreezeing the cell when needs, can unscrew sealed lid, pulls out in the middle of managing from freezing to deposit through the tensile cytomixis liquid that will solidify icy state of personally experiencing sth. Part of the body that sealed lid is connected, cuts in the middle of cell liquid that drawing will the state of freezing directly puts into 37 DEG C the solution that has the culture medium, unfreeze fast, the utility model discloses convenient to use, the quick recovery cell that unfreezes of ability has been avoided unfreezeing for a long time, and the low temperature pair cell causes the harm.
Description
Technical field
The utility model belongs to surgery article, is specifically related to a kind of cryopreservation tube of quick-thawing.
Background technology
Lower than under the condition of ultralow temperature of-70 DEG C, the biochemical reaction of organism cell interior is extremely slow, even stops.Therefore, take suitable method that biomaterial is down to ultralow temperature, vital movement can be made to be fixed on certain one-phase and not aging death.When in a suitable approach frozen biomaterial being returned to normal temperature, the biochemical reaction of its inside can recover normal.So-called freezen protective; exactly culture in vitro thing or bioactive materials are suspended in and are added with or do not add in the solution of cryoprotector; subzero a certain temperature (being generally the condition of ultralow temperature lower than-70 DEG C) is down to certain freezing rate, and at this temperature to the process that it is preserved for a long time.And recovery is exactly the process with certain rewarming rate, frozen culture in vitro thing or bioactive materials being returned to normal temperature.No matter be microorganism, zooblast, plant cell or the organ of culture in vitro can carry out frozen, and recover under proper condition.
If in resuscitation process, the overlong time of thawing, can cause low temperature to cause damage to cell, serious may cause thawing recover unsuccessfully, the cell of preservation is elaborated in loss.
Utility model content
In order to overcome above-mentioned the deficiencies in the prior art, the purpose of this utility model is to provide a kind of cryopreservation tube of quick-thawing, can thaw fast, prevents from thawing for a long time recovering causing damage to cell.
The technical solution adopted in the utility model is: comprise cryopreservation tube, plug differentiated by seal cover, extrusion, label, described extrusion upper end connects seal cover, lower end is goed deep in the middle of cryopreservation tube, described seal cover is spun on cryopreservation tube upper end, and described label differentiates that plug is positioned in the middle of the groove of the outer upper surface of seal cover.
Further, described cryopreservation tube comprises body, base, screw thread, and described body is cylindrical hollow body, and its bottom is sphere, and base is filled circles cylinder, and described screw thread is in the upper end of body, and base connects as one in the lower end of body and body.
Further, described seal cover comprises lid, anti-slip veins cover, indicates groove, loose slot, annular bayonet socket, internal thread, described anti-slip veins cover is enclosed within outside body, represent that groove is at the outer upper surface of lid, described loose slot is in lid in lower surface, outside the loose slot of described annular bayonet socket lower surface in lid, inside described lid, there is internal thread.
Further, described extrusion comprises rotary body, tensile bars, spherosome, and rotary body is fixed in seal cover, freely can rotate in seal cover, described tensile bars and spherosome are all deep in the middle of cryopreservation tube, and described rotary body is in tensile bars upper end, and spherosome is in the lower end of extrusion.
Further, described label differentiates that plug is cylinder, has various marker color
The beneficial effects of the utility model:
The cryopreservation tube of a kind of quick-thawing that the utility model provides is with extrusion, extrusion movable upper end is arranged in the interior loose slot of seal cover, can be freely movable, when the sealing of rotatory sealing lid or when opening cryopreservation tube, extrusion can not be made to rotate together along with seal cover, tensile bars and spherosome are deep in cryopreservation tube, when the cell mixture in cryopreservation tube solidify freezing after, spherosome and part extrusion by be rolled in freezing state cell mixture in the middle of, the medium that extrusion can be used to be pulled out in the middle of cryopreservation tube by the cell mixture of freezing curdled appearance be then directly placed on 37 DEG C when recovery easily in the middle of thaw rapidly, this method thaws recovery efficiently much than traditional being placed on together in water by cryopreservation tube, can directly thaw to cell mixture ice cube, thaw recovery rapidly, avoiding thaws for a long time causes damage to cell.
Accompanying drawing explanation
Fig. 1 is the cryopreservation pipe structure schematic diagram of a kind of quick-thawing that the utility model proposes;
Fig. 2 is the cryopreservation tube cross-sectional view of a kind of quick-thawing that the utility model proposes;
Fig. 3 is the cryopreservation tube sealing cover structure schematic diagram of a kind of quick-thawing that the utility model proposes.
Description of reference numerals
1-cryopreservation tube, 2-seal cover, 3-extrusion, plug differentiated by 4-label, 11-body, 12-base, 13-body screw thread, 21-lid, 22-anti-slip veins cover, and 23-indicates groove, 24-loose slot, 25-annular bayonet socket, 26 internal threads, 31-activity topic.32-tensile bars, 33-spherosome.
Embodiment
Below in conjunction with accompanying drawing, the utility model is described further.
See Fig. 1, it is a kind of cryopreservation pipe structure schematic diagram of quick-thawing that the utility model proposes.
As shown in Figure 1, a kind of cryopreservation tube of quick-thawing, comprise cryopreservation tube (1), plug (4) differentiated by seal cover (2), extrusion (3), label, described extrusion (3) upper end connects seal cover (2), lower end is goed deep in the middle of cryopreservation tube (1), described seal cover (2) is spun on cryopreservation tube (1) upper end, and described label differentiates that plug (4) is positioned in the middle of the groove of seal cover (2) outer upper surface.
In the present embodiment, after cultured cell mixture injects in the middle of cryopreservation tube (1), first extrusion (3) is put in the middle of cryopreservation tube (1), then screwing hermetic lid (2), just can be placed into cryopreservation tube in the middle of Cryo Equipment after buckling into label discriminating plug (4) of appropriate color and preserve cell; When recovery, cryopreservation tube is taken out in the middle of Cryo Equipment, turn on seal cover (2), use extrusion (3) by the cell sap of freezing state pull-out cryopreservation tube (1), in the middle of the cell culture fluid directly putting into 37 DEG C, directly thaw, can thaw cell mixture so fast, prevents from having thawed slowly causing damage to cell.
In the present embodiment, the material of the utility model cryopreservation tube does not limit, and selects, be as the criterion to realize the technical program with the frozen temperature of reality and the antipollution needs of health.
As shown in Figure 2, described cryopreservation tube (1) comprises body (11), base (12), body screw thread (13), described body (11) is cylindrical hollow body, body (11) bottom is sphere, base (12) is filled circles cylinder, described body screw thread (13) is in the upper end of body (11), and base (12) connects as one in the lower end of body (11) and body (11).
In the utility model, body (11) bottom is concave spherical surface, after cell mixture is freezing, greatly reduce the resistance between ice cube and tube body wall, can very easily by the pull-out in the middle of cryopreservation tube (1) of the cell mixture of freezing state.
As shown in Figure 3, described seal cover (2) comprises lid (21), anti-slip veins cover (22), indicate groove (23), loose slot (24), annular bayonet socket (25), internal thread (26), described anti-slip veins cover (22) is enclosed within lid (21) outside, indicate groove (23) at lid (21) outer upper surface, described loose slot (24) is in lid (21) in lower surface, outside the loose slot of described annular bayonet socket (25) lower surface in lid (21), there is internal thread (26) described lid (21) inner side.
In the utility model embodiment, the material of seal cover (2) does not limit, to realize being used new technique scheme to be as the criterion.
In the utility model embodiment, anti-slip veins cover (22) is connected with lid (21) one, there is anti-slip veins cover (22) that frictional force between staff and seal cover (2) can be increased, convenient rotatory sealing lid (2) easily.
In the utility model embodiment, loose slot (24) is had in lid (21), extrusion (3) rotary body (31) is arranged in loose slot (24), after the spherosome (33) of extrusion (3) and tensile bars (32) are frozen by cell sap, time rotatory sealing lid (2) opens cryopreservation tube (1), extrusion (3) is due to the effect of rotary body (31), and then can not rotate along with the rotation of seal cover (2), avoid twisting into two parts tensile bars (32), be inconvenient to the cell mixture pulling out freezing state.
As shown in Figure 2, described extrusion (3) comprises rotary body (31), tensile bars (32), spherosome (33), rotary body (31) is fixed in seal cover (2), freely can rotate in seal cover (2), described tensile bars (32) and spherosome (33) are all deep in the middle of cryopreservation tube (1), described rotary body (31) is in tensile bars (32) upper end, and spherosome (33) is in the lower end of extrusion (32).
Further, described label differentiates that plug (4) is cylinder, has various marker color.
In the middle of the utility model, extrusion (3) is deep in the middle of cryopreservation tube (1), when the recovery cell that thaws, the pull-out in the middle of cryopreservation tube (1) of the cell mixture of freezing state can be put in the middle of the cell culture fluid of 37 DEG C by extrusion (3), directly carry out directly thawing fast to the cell mixture of freezing state, preventing from thawing for a long time causes damage to cell.
By reference to the accompanying drawings the utility model preferred embodiment is explained in detail above, but the utility model is not limited to above-mentioned embodiment, in the ken that those of ordinary skill in the art possess, can also make a variety of changes under the prerequisite not departing from the utility model aim.Do not depart from design of the present utility model and scope can make many other and change and remodeling.Should be appreciated that the utility model is not limited to specific embodiment, scope of the present utility model is defined by the following claims.
Claims (5)
1. the cryopreservation tube of a quick-thawing, it is characterized in that, comprise: cryopreservation tube (1), plug (4) differentiated by seal cover (2), extrusion (3), label, described extrusion (3) upper end connects seal cover (2), lower end is goed deep in the middle of cryopreservation tube (1), described seal cover (2) is spun on cryopreservation tube (1) upper end, and described label differentiates that plug (4) is positioned in the middle of the groove of seal cover (2) outer upper surface.
2. the cryopreservation tube of a kind of quick-thawing according to claim 1, it is characterized in that, described cryopreservation tube (1) comprises body (11), base (12), body screw thread (13), described body (11) is cylindrical hollow body, body (11) bottom is sphere, base (12) is filled circles cylinder, described body screw thread (13) is in the upper end of body (11), and base (12) connects as one in the lower end of body (11) and body (11).
3. the cryopreservation tube of a kind of quick-thawing according to claim 1, it is characterized in that, described seal cover (2) comprises lid (21), anti-slip veins cover (22), indicate groove (23), loose slot (24), annular bayonet socket (25), internal thread (26), described anti-slip veins cover (22) is enclosed within lid (21) outside, indicate groove (23) at lid (21) outer upper surface, described loose slot (24) is in lid (21) in lower surface, outside the loose slot of described annular bayonet socket (25) lower surface in lid (21), there is internal thread (26) described lid (21) inner side.
4. the cryopreservation tube of a kind of quick-thawing according to claim 1, it is characterized in that, described extrusion (3) comprises rotary body (31), tensile bars (32), spherosome (33), rotary body (31) is fixed in seal cover (2), freely can rotate in seal cover (2), described tensile bars (32) and spherosome (33) are all deep in the middle of cryopreservation tube (1), described rotary body (31) is in tensile bars (32) upper end, and spherosome (33) is in the lower end of extrusion (32).
5. the cryopreservation tube of a kind of quick-thawing according to claim 1, is characterized in that, described label differentiates that plug (4) is cylinder, has various marker color.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520752653.5U CN205071975U (en) | 2015-09-25 | 2015-09-25 | Pipe is deposited in freezing of unfreezeing fast |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520752653.5U CN205071975U (en) | 2015-09-25 | 2015-09-25 | Pipe is deposited in freezing of unfreezeing fast |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205071975U true CN205071975U (en) | 2016-03-09 |
Family
ID=55424432
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520752653.5U Expired - Fee Related CN205071975U (en) | 2015-09-25 | 2015-09-25 | Pipe is deposited in freezing of unfreezeing fast |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN205071975U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467012A (en) * | 2017-08-23 | 2017-12-15 | 中国人民解放军第四军医大学 | A kind of cryopreservation tube and freezing storing box for inverting fast sampling |
-
2015
- 2015-09-25 CN CN201520752653.5U patent/CN205071975U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107467012A (en) * | 2017-08-23 | 2017-12-15 | 中国人民解放军第四军医大学 | A kind of cryopreservation tube and freezing storing box for inverting fast sampling |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160309 |