CN103975914B - Long-term preservation device for biological sample through gasified liquid nitrogen - Google Patents
Long-term preservation device for biological sample through gasified liquid nitrogen Download PDFInfo
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- CN103975914B CN103975914B CN201410182496.9A CN201410182496A CN103975914B CN 103975914 B CN103975914 B CN 103975914B CN 201410182496 A CN201410182496 A CN 201410182496A CN 103975914 B CN103975914 B CN 103975914B
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Abstract
The invention relates to a long-term preservation device for biological samples through gasified liquid nitrogen. The device includes a cryogenic storage box. Each of an upper part and a lower part of the cryogenic storage box is provided with a first heat-insulation layer. A central gas-ejection pipe is disposed in the cryogenic storage box in a penetrating manner and is connected to the cryogenic storage box through a rotating bearing. A cryogenic chamber is formed between the cryogenic storage box and the central gas-ejection pipe. A temperature probe is disposed in the cryogenic chamber. The central gas-ejection pipe is provided with a gas-ejection mouth. Each of an upper end and a lower end of the central gas-ejection pipe is arranged at an exterior of the first heat-insulation layer. One end, which is positioned at an exterior of the upper first-insulation layer, of the central gas-ejection pipe is a liquid nitrogen inlet and is connected to a control valve; and the other end, which is positioned at the exterior of the lower first-insulation layer, of the central gas-ejection pipe is a liquid nitrogen outlet and is connected to a pressure control valve. The device is suitable for large-scale numerical control preservation and management of biological samples; meanwhile, irretrievable sample loss due to liquid nitrogen consumption can be avoided.
Description
Technical field
The present invention relates to a kind of biological sample storage device, particularly a kind of device being preserved biological sample by gasification liquid nitrogen for a long time.
Background technology
Liquid nitrogen, in bio-pharmaceuticals, is commonly used in medical and health industry as the long-term cold medium preserving biological sample.Biological sample is positioned in sealing liquid nitrogen container by traditional Liquid nitrogen storage technology.Because liquid nitrogen can volatilize along with the time, regular check liquid nitrogen horizontal is the committed step in management.Along with a large amount of biological samples, as stem cell, start in a planned way to carry out long term storage.The Cord blood technology of this original extensive style, was both unfavorable for management on large, especially numerical control management, also greatly increase keeping cost, and once because of mismanagement, cause liquid nitrogen consumption, the biological sample loss consequence caused cannot make up.
Summary of the invention
The object of the invention is to overcome above deficiency, providing a kind of and being applicable to the preservation of extensive numerical control biological sample and management use, the device being preserved biological sample by gasification liquid nitrogen for a long time that liquid nitrogen can not be caused to waste.
Object of the present invention is achieved through the following technical solutions: a kind of device being preserved biological sample by gasification liquid nitrogen for a long time, comprise deep cooling storage bin, the top and the bottom of deep cooling storage bin are equipped with the first heat-insulation layer, run through in deep cooling storage bin and be provided with central air jet pipe, be connected by rolling bearing between deep cooling storage bin with central air jet pipe, chilling chamber is formed between deep cooling storage bin and central air jet pipe, temp probe is provided with in chilling chamber, central authorities' air jet pipe is provided with puff prot, the two ends up and down of central authorities' air jet pipe are all placed in the outside of the first heat-insulation layer, one end that central authorities' air jet pipe is positioned at heat-insulation layer outside, top first is liquid nitrogen import and is connected with control valve, one end that central authorities' air jet pipe is positioned at heat-insulation layer outside, bottom first is nitrogen outlet and is connected with pressure-control valve.
Further improvement of the present invention is: described deep cooling storage bin comprises multilayer deep cooling box group, often organizes deep cooling box group and includes multiple deep cooling box arranged around central air jet pipe.
Further improvement of the present invention is: each described deep cooling box comprises box body, deep cooling door with the second heat-insulation layer, and be connected by rotating shaft between box body with deep cooling door, the back of deep cooling box opens to chilling chamber, the outwards opening of deep cooling door.
Further improvement of the present invention is: it is characterized in that: the below of each described deep cooling box is equipped with dragging track.
The present invention compared with prior art has the following advantages: use more for convenience, the temperature of each deep cooling box can well be ensured, and be convenient to pick and place sample, facilitate extensive numerical control to manage, save production cost, avoid the loss of the biological sample that the consumption due to liquid nitrogen causes.
accompanying drawing illustrates:
Fig. 1 is structural representation of the present invention;
Fig. 2 be in Fig. 1 A-A to structural representation;
Fig. 3 is the structural representation of deep cooling box;
Number in the figure: 1-deep cooling storage bin, 2-first heat-insulation layer, 3-central authorities air jet pipe, 4-rolling bearing, 5-chilling chamber, 6-puff prot, 7-deep cooling box group, 8-deep cooling box, 9-box body, 10-second heat-insulation layer, 11-deep cooling door, the import of 12-liquid nitrogen, 13-control valve, 14-nitrogen outlet, 15-pressure-control valve, 16-temp probe, 17-rotating shaft.
embodiment:
In order to deepen the understanding of the present invention, below in conjunction with embodiment and accompanying drawing, the invention will be further described, and this embodiment only for explaining the present invention, does not form limiting the scope of the present invention.
As Fig. 1 to Fig. 3 shows a kind of embodiment of being preserved the device of biological sample by gasification liquid nitrogen for a long time of the present invention, comprise deep cooling storage bin 1, the top and the bottom of deep cooling storage bin 1 are equipped with the first heat-insulation layer 2, run through in deep cooling storage bin 1 and be provided with central air jet pipe 3, be connected by rolling bearing 4 between deep cooling storage bin 1 with central air jet pipe 3, chilling chamber 5 is formed between deep cooling storage bin 1 and central air jet pipe 3, temp probe 16 is provided with in chilling chamber 5, central authorities' air jet pipe 3 is provided with puff prot 6, the two ends up and down of central authorities' air jet pipe 3 are all placed in the outside of the first heat-insulation layer 2, one end that central authorities' air jet pipe 3 is positioned at heat-insulation layer 2 outside, top first is liquid nitrogen import 12 and is connected with control valve 13, one end that central authorities' air jet pipe 3 is positioned at heat-insulation layer 2 outside, bottom first is nitrogen outlet 14 and is connected with pressure-control valve 15, the preferred deep cooling storage bin 1 of the present invention comprises multilayer deep cooling box group 7, often organize deep cooling box group 7 and include multiple deep cooling box 8 arranged around central air jet pipe 3, each deep cooling box 8 comprises box body 9, with the deep cooling door 11 of the second heat-insulation layer 10, be connected by rotating shaft 17 between box body 8 with deep cooling door 11, the back of deep cooling box 8 opens to chilling chamber 5, deep cooling door 11 outwards opening, the below of each deep cooling box 8 is equipped with dragging track.
Biological sample storage device of the present invention adopts continuous jet-propelled deep cooling store method to preserve, this device does not directly adopt Liquid nitrogen storage, suitable storage temperature should be slightly higher than the condensing temperature-196 DEG C of liquid nitrogen about 5-10 DEG C, its actual temp is arranged by central control room and controls, liquid nitrogen is sent into from the liquid nitrogen import 12 of central air jet pipe 3, the puff prot 6 sprayed paint on pipe 3 by central authorities enters chilling chamber, gas is discharged by the nitrogen outlet 14 below pipeline after exceeding certain pressure, each deep cooling box back is open towards chilling chamber 5, to keep minimum temperature, sliding rail is provided with below deep cooling box, be convenient to pick and place sample, 11, deep cooling door can outwards opening, to pick and place sample at any time, rolling bearing 4 is had to connect between central authorities' air jet pipe and deep cooling storage bin, whole like this deep cooling storage bin can rotate around central air jet pipe 3, be convenient to pick and place sample, whole device uses more for convenience.
Claims (4)
1. preserved the device of biological sample for a long time by gasification liquid nitrogen for one kind, it is characterized in that: comprise deep cooling storage bin (1), the top and the bottom of described deep cooling storage bin (1) are equipped with the first heat-insulation layer (2), run through in described deep cooling storage bin (1) and be provided with central air jet pipe (3), be connected by rolling bearing (4) between described deep cooling storage bin (1) with described central air jet pipe (3), chilling chamber (5) is formed between described deep cooling storage bin (1) and described central air jet pipe (3), temp probe (16) is provided with in described chilling chamber (5), described central air jet pipe (3) is provided with puff prot (6), the two ends up and down of described central air jet pipe (3) are all placed in the outside of described first heat-insulation layer (2), one end that described central air jet pipe (3) is positioned at the first heat-insulation layer (2) described in top outside is liquid nitrogen import (12) and is connected with control valve (13), one end that described central air jet pipe (3) is positioned at the first heat-insulation layer (2) described in bottom outside is nitrogen outlet (14) and is connected with pressure-control valve (15).
2. a kind ofly according to claim 1 preserve the device of biological sample for a long time by gasification liquid nitrogen, it is characterized in that: described deep cooling storage bin (1) comprises multilayer deep cooling box group (7), often organize described deep cooling box group (7) and include multiple deep cooling box (8) arranged around described central air jet pipe (3).
3. a kind ofly according to claim 2 preserve the device of biological sample for a long time by gasification liquid nitrogen, it is characterized in that: each described deep cooling box (8) comprises box body (9), deep cooling door (11) with the second heat-insulation layer (10), be connected by rotating shaft (17) between described box body (9) with described deep cooling door (11), the back of described deep cooling box (9) opens to described chilling chamber (5), the outwards opening of described deep cooling door (11).
4. a kind ofly according to claim 3 preserve the device of biological sample for a long time by gasification liquid nitrogen, it is characterized in that: the below of each described deep cooling box (8) is equipped with dragging track.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410182496.9A CN103975914B (en) | 2014-05-04 | 2014-05-04 | Long-term preservation device for biological sample through gasified liquid nitrogen |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410182496.9A CN103975914B (en) | 2014-05-04 | 2014-05-04 | Long-term preservation device for biological sample through gasified liquid nitrogen |
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| CN103975914A CN103975914A (en) | 2014-08-13 |
| CN103975914B true CN103975914B (en) | 2015-05-13 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886037B (en) * | 2015-05-06 | 2017-01-18 | 中南大学 | Biological sample simulative preservation experiment device and biological sample preservation method |
| CN106742757A (en) * | 2016-12-31 | 2017-05-31 | 广东雨嘉水产食品有限公司 | A kind of case inner structure for freezing Tilapia mossambica antistaling box |
| CN107328151B (en) * | 2017-08-09 | 2023-01-06 | 南通天鼎信息咨询有限公司 | Biological sample long-term ultra-low temperature keeps cabinet |
| CN108168177A (en) * | 2018-01-29 | 2018-06-15 | 南通天工深冷新材料强化有限公司 | A kind of new bio sample preservation cabinet |
| CN111750611A (en) * | 2020-07-20 | 2020-10-09 | 宁波精酿谷科技有限公司 | Refrigerating device |
| CN214853921U (en) * | 2021-05-11 | 2021-11-26 | 上海原能细胞生物低温设备有限公司 | Sample cooling storage mechanism |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN2740615Y (en) * | 2004-05-18 | 2005-11-16 | 武圣明 | Food steamer style liquid nitrogen freezing storing box |
| CN203860327U (en) * | 2014-05-04 | 2014-10-08 | 南通天工深冷新材料强化有限公司 | Device for long-term preservation of biological samples through gasified liquid nitrogen |
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Effective date of registration: 20220112 Address after: 226000 floor 2, block C, No. 128, Changtai Road, Chongchuan District, Nantong City, Jiangsu Province Patentee after: Nantong Tianding Information Consulting Co.,Ltd. Address before: 226000 room 206, block C, No. 128, Changtai Road, Gangzha District, Nantong City, Jiangsu Province Patentee before: NANTONG CRYOTHERM TIANGONG CO.,LTD. |
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Effective date of registration: 20220819 Address after: Room 301, No. 10, Jiuling Road, Yonghe Economic Zone, Huangpu District, Guangzhou City, Guangdong Province, 510000 Patentee after: Guangzhou Cosmo Biotechnology Co.,Ltd. Address before: 226000 floor 2, block C, No. 128, Changtai Road, Chongchuan District, Nantong City, Jiangsu Province Patentee before: Nantong Tianding Information Consulting Co.,Ltd. |