CN103975914A - Long-term preservation device for biological sample through gasified liquid nitrogen - Google Patents
Long-term preservation device for biological sample through gasified liquid nitrogen Download PDFInfo
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- CN103975914A CN103975914A CN201410182496.9A CN201410182496A CN103975914A CN 103975914 A CN103975914 A CN 103975914A CN 201410182496 A CN201410182496 A CN 201410182496A CN 103975914 A CN103975914 A CN 103975914A
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- deep cooling
- liquid nitrogen
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- air jet
- insulation layer
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Abstract
The invention relates to a long-term preservation device for biological samples through gasified liquid nitrogen. The device includes a cryogenic storage box. Each of an upper part and a lower part of the cryogenic storage box is provided with a first heat-insulation layer. A central gas-ejection pipe is disposed in the cryogenic storage box in a penetrating manner and is connected to the cryogenic storage box through a rotating bearing. A cryogenic chamber is formed between the cryogenic storage box and the central gas-ejection pipe. A temperature probe is disposed in the cryogenic chamber. The central gas-ejection pipe is provided with a gas-ejection mouth. Each of an upper end and a lower end of the central gas-ejection pipe is arranged at an exterior of the first heat-insulation layer. One end, which is positioned at an exterior of the upper first-insulation layer, of the central gas-ejection pipe is a liquid nitrogen inlet and is connected to a control valve; and the other end, which is positioned at the exterior of the lower first-insulation layer, of the central gas-ejection pipe is a liquid nitrogen outlet and is connected to a pressure control valve. The device is suitable for large-scale numerical control preservation and management of biological samples; meanwhile, irretrievable sample loss due to liquid nitrogen consumption can be avoided.
Description
Technical field
The present invention relates to a kind of biological sample storage device, particularly a kind of device of preserving for a long time biological sample by gasification liquid nitrogen.
Background technology
Liquid nitrogen, in bio-pharmaceuticals, is generally used as the cold medium of long-term preservation biological sample in medical and health industry.Traditional liquid nitrogen Techniques of preserving is positioned over biological sample in sealing liquid nitrogen container.Because liquid nitrogen can volatilize along with the time, regular check liquid nitrogen horizontal is the committed step in management.Along with a large amount of biological samples, as stem cell, start in a planned way to carry out long term storage.The low temperature Techniques of preserving of this original extensive style, had both been unfavorable for management on large, and especially numerical control management also increases keeping cost greatly, once and because of mismanagement, cause liquid nitrogen consumption, the biological sample loss consequence causing cannot make up.
Summary of the invention
The object of the invention is in order to overcome above deficiency, a kind of be applicable to extensive numerical control biological sample preservation and management use are provided, can not cause the device that gasification liquid nitrogen is preserved biological sample for a long time that passes through of liquid nitrogen waste.
Object of the present invention is achieved through the following technical solutions: a kind of device of preserving for a long time biological sample by gasification liquid nitrogen, comprise deep cooling storage bin, the top and the bottom of deep cooling storage bin are equipped with the first heat-insulation layer, in deep cooling storage bin, run through and be provided with central air jet pipe, between deep cooling storage bin and central air jet pipe, by rolling bearing, be connected, between deep cooling storage bin and central air jet pipe, form chilling chamber, in chilling chamber, be provided with temp probe, central authorities' air jet pipe is provided with puff prot, the two ends up and down of central authorities' air jet pipe are all placed in the outside of the first heat-insulation layer, one end that central authorities' air jet pipe is positioned at the first heat-insulation layer outside, top is liquid nitrogen import and is connected with control valve, one end that central authorities' air jet pipe is positioned at the first heat-insulation layer outside, bottom is nitrogen outlet and is connected with pressure-control valve.
Further improvement of the present invention is: described deep cooling storage bin comprises multilayer deep cooling box group, and every group of deep cooling box group includes a plurality of deep cooling boxes that arrange around central air jet pipe.
Further improvement of the present invention is: described in each deep cooling box comprise box body, with the deep cooling door of the second heat-insulation layer, between box body and deep cooling door, by rotating shaft, be connected, the back of deep cooling box is opened to chilling chamber, deep cooling door is outwards open.
Further improvement of the present invention is: it is characterized in that: described in each, the below of deep cooling box is equipped with dragging track.
The present invention compared with prior art has the following advantages: use more for convenience, can well guarantee the temperature of each deep cooling box, and be convenient to pick and place sample, facilitate extensive numerical control management, save production cost, avoided the loss of the biological sample that the consumption due to liquid nitrogen causes.
accompanying drawing explanation:
Fig. 1 is structural representation of the present invention;
Fig. 2 be in Fig. 1 A-A to structural representation;
Fig. 3 is the structural representation of deep cooling box;
Number in the figure: 1-deep cooling storage bin, 2-the first heat-insulation layer, 3-central authorities air jet pipe, 4-rolling bearing, 5-chilling chamber, 6-puff prot, 7-deep cooling box group, 8-deep cooling box, 9-box body, 10-the second heat-insulation layer, 11-deep cooling door, the import of 12-liquid nitrogen, 13-control valve, 14-nitrogen outlet, 15-pressure-control valve, 16-temp probe, 17-rotating shaft.
embodiment:
In order to deepen the understanding of the present invention, below in conjunction with embodiment and accompanying drawing, the invention will be further described, and this embodiment only, for explaining the present invention, does not form limiting the scope of the present invention.
As Fig. 1 to Fig. 3 shows a kind of embodiment of preserving for a long time the device of biological sample by gasification liquid nitrogen of the present invention, comprise deep cooling storage bin 1, the top and the bottom of deep cooling storage bin 1 are equipped with the first heat-insulation layer 2, in deep cooling storage bin 1, run through and be provided with central air jet pipe 3, between deep cooling storage bin 1 and central air jet pipe 3, by rolling bearing 4, be connected, between deep cooling storage bin 1 and central air jet pipe 3, form chilling chamber 5, in chilling chamber 5, be provided with temp probe 16, central authorities' air jet pipe 3 is provided with puff prot 6, the two ends up and down of central authorities' air jet pipe 3 are all placed in the outside of the first heat-insulation layer 2, one end that central authorities' air jet pipe 3 is positioned at the first heat-insulation layer 2 outsides, top is liquid nitrogen import 12 and is connected with control valve 13, one end that central authorities' air jet pipe 3 is positioned at the first heat-insulation layer 2 outsides, bottom is nitrogen outlet 14 and is connected with pressure-control valve 15, the preferred deep cooling storage bin 1 of the present invention comprises multilayer deep cooling box group 7, every group of deep cooling box group 7 includes a plurality of deep cooling boxes 8 that arrange around central air jet pipe 3, each deep cooling box 8 comprises box body 9, deep cooling door 11 with the second heat-insulation layer 10, between box body 8 and deep cooling door 11, by rotating shaft 17, be connected, the back of deep cooling box 8 is opened to chilling chamber 5, deep cooling door 11 is outwards open, the below of each deep cooling box 8 is equipped with dragging track.
Biological sample storage device of the present invention adopts continuous jet-propelled deep cooling store method to preserve, this device does not directly adopt liquid nitrogen to preserve, suitable storage temperature should be than ℃ slightly high 5-10 ℃ left and right, condensing temperature-196 of liquid nitrogen, its actual temp is arranged and is controlled by central control room, liquid nitrogen is sent into from the liquid nitrogen import 12 of central air jet pipe 3, the puff prot 6 spraying paint on pipe 3 by central authorities enters chilling chamber, gas is discharged by the nitrogen outlet 14 below pipeline after surpassing certain pressure, each deep cooling box back is open towards chilling chamber 5, to keep minimum temperature, deep cooling box below is provided with sliding rail, be convenient to pick and place sample, 11, deep cooling door can outwards be opened, to pick and place at any time sample, between central authorities' air jet pipe and deep cooling storage bin, there is rolling bearing 4 to connect, whole like this deep cooling storage bin can rotate around central air jet pipe 3, be convenient to pick and place sample, whole device is used more for convenience.
Claims (4)
1. by gasification liquid nitrogen, preserve for a long time the device of biological sample for one kind, it is characterized in that: comprise deep cooling storage bin (1), the top and the bottom of described deep cooling storage bin (1) are equipped with the first heat-insulation layer (2), in described deep cooling storage bin (1), run through and be provided with central air jet pipe (3), between described deep cooling storage bin (1) and described central air jet pipe (3), by rolling bearing (4), be connected, between described deep cooling storage bin (1) and described central air jet pipe (3), form chilling chamber (5), in described chilling chamber (5), be provided with temp probe (16), described central air jet pipe (3) is provided with puff prot (6), the two ends up and down of described central air jet pipe (3) are all placed in the outside of described the first heat-insulation layer (2), described central air jet pipe (3) is positioned at outside one end of the first heat-insulation layer (2) described in top and is liquid nitrogen import (12) and is connected with control valve (13), described central air jet pipe (3) is positioned at outside one end of the first heat-insulation layer (2) described in bottom and is nitrogen outlet (14) and is connected with pressure-control valve (15).
2. a kind ofly according to claim 1 by gasification liquid nitrogen, preserve for a long time the device of biological sample, it is characterized in that: described deep cooling storage bin (1) comprises multilayer deep cooling box group (7), described in every group, deep cooling box group (7) includes a plurality of deep cooling boxes (8) that arrange around described central air jet pipe (3).
3. a kind ofly according to claim 2 by gasification liquid nitrogen, preserve for a long time the device of biological sample, it is characterized in that: described in each deep cooling box (8) comprise box body (9), with the deep cooling door (11) of the second heat-insulation layer (10), between described box body (8) and described deep cooling door (11), by rotating shaft (17), be connected, the back of described deep cooling box (8) is opened to described chilling chamber (5), and described deep cooling door (11) is outwards open.
4. a kind ofly according to claim 3 by gasification liquid nitrogen, preserve for a long time the device of biological sample, it is characterized in that: described in each, the below of deep cooling box (8) is equipped with dragging track.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410182496.9A CN103975914B (en) | 2014-05-04 | 2014-05-04 | Long-term preservation device for biological sample through gasified liquid nitrogen |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410182496.9A CN103975914B (en) | 2014-05-04 | 2014-05-04 | Long-term preservation device for biological sample through gasified liquid nitrogen |
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| CN103975914A true CN103975914A (en) | 2014-08-13 |
| CN103975914B CN103975914B (en) | 2015-05-13 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886037A (en) * | 2015-05-06 | 2015-09-09 | 中南大学 | Biological sample simulative preservation experiment device and biological sample preservation method |
| CN106742757A (en) * | 2016-12-31 | 2017-05-31 | 广东雨嘉水产食品有限公司 | A kind of case inner structure for freezing Tilapia mossambica antistaling box |
| CN107328151A (en) * | 2017-08-09 | 2017-11-07 | 南通天工深冷新材料强化有限公司 | A kind of long-term ultralow temperature preservation cabinet of biological sample |
| CN108168177A (en) * | 2018-01-29 | 2018-06-15 | 南通天工深冷新材料强化有限公司 | A kind of new bio sample preservation cabinet |
| CN111750611A (en) * | 2020-07-20 | 2020-10-09 | 宁波精酿谷科技有限公司 | Refrigerating device |
| WO2022237344A1 (en) * | 2021-05-11 | 2022-11-17 | 上海原能细胞生物低温设备有限公司 | Sample cooling and storage mechanism |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2740615Y (en) * | 2004-05-18 | 2005-11-16 | 武圣明 | Food steamer style liquid nitrogen freezing storing box |
| CN203860327U (en) * | 2014-05-04 | 2014-10-08 | 南通天工深冷新材料强化有限公司 | Device for long-term preservation of biological samples through gasified liquid nitrogen |
-
2014
- 2014-05-04 CN CN201410182496.9A patent/CN103975914B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2740615Y (en) * | 2004-05-18 | 2005-11-16 | 武圣明 | Food steamer style liquid nitrogen freezing storing box |
| CN203860327U (en) * | 2014-05-04 | 2014-10-08 | 南通天工深冷新材料强化有限公司 | Device for long-term preservation of biological samples through gasified liquid nitrogen |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104886037A (en) * | 2015-05-06 | 2015-09-09 | 中南大学 | Biological sample simulative preservation experiment device and biological sample preservation method |
| CN106742757A (en) * | 2016-12-31 | 2017-05-31 | 广东雨嘉水产食品有限公司 | A kind of case inner structure for freezing Tilapia mossambica antistaling box |
| CN107328151A (en) * | 2017-08-09 | 2017-11-07 | 南通天工深冷新材料强化有限公司 | A kind of long-term ultralow temperature preservation cabinet of biological sample |
| CN107328151B (en) * | 2017-08-09 | 2023-01-06 | 南通天鼎信息咨询有限公司 | Biological sample long-term ultra-low temperature keeps cabinet |
| CN108168177A (en) * | 2018-01-29 | 2018-06-15 | 南通天工深冷新材料强化有限公司 | A kind of new bio sample preservation cabinet |
| CN111750611A (en) * | 2020-07-20 | 2020-10-09 | 宁波精酿谷科技有限公司 | Refrigerating device |
| WO2022237344A1 (en) * | 2021-05-11 | 2022-11-17 | 上海原能细胞生物低温设备有限公司 | Sample cooling and storage mechanism |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103975914B (en) | 2015-05-13 |
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