CN204811679U - Simple and easy multipurpose procedure cooling box - Google Patents
Simple and easy multipurpose procedure cooling box Download PDFInfo
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- CN204811679U CN204811679U CN201520557367.3U CN201520557367U CN204811679U CN 204811679 U CN204811679 U CN 204811679U CN 201520557367 U CN201520557367 U CN 201520557367U CN 204811679 U CN204811679 U CN 204811679U
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Abstract
The utility model discloses a simple and easy multipurpose procedure cooling box, including cooling box main part, keep warm lid and sample groove, the lid that keeps warm is located the top of cooling box main part, sample trench in the inside of cooling box main part, the rectangle sample groove and the cylindrical sample groove of depositing the pipe is frozen including being used for depositing different models in the sample groove, the cooperation through thread sealing is covered with keeping warm to cooling box main part. The utility model discloses not only can be used for freezing save of summary procedure unicellular or that organize the type, can also satisfy great volume cell suspension's cooling and preserve and the multiple freezing needs of preserving container procedure cooling simultaneously. The thread sealing cooperation is covered in cooling box main part and heat preservation, more helps keeping the stability of cooling box, prevents box body and interior slotware's liquid outflow.
Description
Technical field
The utility model relates to a kind of program temperature reduction box, particularly a kind of Simple multipurpose program temperature reduction box.
Background technology
The freezen protective of cell needs to add suitable cryoprotector, forms ice crystal to prevent the water in temperature-fall period in cell and in environment.Do not add cryoprotector and can cause a series of change in cell, the cellular damage caused as mechanical damage, electrolyte concentration rising, osmotic pressure change, dehydration, pH value change, protein denaturation etc. is even dead.
The most frequently used preserving type of current biological cells and tissues is Cryopreservation (being stored in-196 ° of C), and temperature-fall period becomes the most critical factor determining preservation effect.What the temperature-fall period of current single celled deep-bed drying mainly adopted is slow cooling mode, can realize slow programmed cooling, to ensure the survival of cell by adopting the programmed cooling instrument of conputer controlled.But due to programmed cooling instrument costly, take up an area large, complex operation is complicated, need the shortcomings such as the timely supply of liquid nitrogen, domestic most researcher generally uses refrigerator to lower the temperature step by step, and summary procedure cooling box is progressively applied to the freezing of biological cells and tissues in recent years.
What summary procedure cooling box adopted is that the organic reagents such as isopropyl alcohol are as buffering, at the cell balanced at 4 DEG C and program temperature reduction box are placed in-80 DEG C, the cooling rate of 1 DEG C/min can be realized, substantially meet the needs of cell deep-bed drying slow cooling, and realize the higher Cell viability of frozen rear cell maintenance.
The frozen concentration of cell is generally 5 × 10
5/ mL is to 1 × 10
7between/mL, the frozen excessive concentration of cell or the too low motility rate that all can reduce frozen rear cell, significantly affect refrigerating effect.Along with carrying out of clinical testing and research etc., to the increase of more cell (even can reach 109) demand, the preservation container of more volume just seems particularly important, and equally, the programmed cooling device that can meet corresponding large volume cell cryopreservation is also extremely urgent.Tube seat shape program temperature reduction box conventional at present is only suitable for the cell cryopreservation tube of specific model, cannot meet the demand.
Summary of the invention
For the defect of prior art, the utility model provides a kind of Simple multipurpose program temperature reduction box, not only may be used for summary procedure stored frozen that is unicellular or organization type, the needs of programmed cooling while of the cooling preservation of larger volume cell suspension and multiple freeze storage container can also be met.
In order to solve the technical problem, the technical solution adopted in the utility model is: a kind of Simple multipurpose program temperature reduction box, comprise cooling box main body, insulation cover and sample slot, insulation cover is positioned at the top of cooling box main body, sample slot is positioned at the inside of cooling box main body, and described sample slot comprises rectangle sample slot for depositing different model cryopreservation tube and cylindrical sample slot; Described cooling box main body and insulation cover pass through threaded sealing fit.
Further, the quantity of described rectangle sample slot is 2-5, and length is 140-180mm, and width is 10-20mm, and the degree of depth is 50-80mm; Described cylindrical sample slot has two profiles number, and a kind of aperture is 10-30mm, and hole depth is 40-60mm, and another kind of aperture is 10-30mm, and hole depth is 30-50mm, and the quantity of cylindrical sample slot is 10-20.
Further, described cooling box main body is combined by thread seal with sample slot.
Further, described cooling box main body is cylindrical.
Further, described cooling box main body, insulation cover and sample slot all use polythene PE material to make.
The beneficial effects of the utility model: Simple multipurpose program temperature reduction box described in the utility model comprises cooling box main body, insulation cover and sample slot, described sample slot comprises rectangle sample slot and cylindrical sample slot, cylindrical sample slot has two profiles number, not only may be used for summary procedure freezen protective that is unicellular or organization type, the needs of programmed cooling while of the cooling preservation of larger volume cell suspension and multiple freeze storage container can also be met.Described cooling box main body and insulation cover threaded sealing fit, more contribute to the stability keeping cooling box, prevent the liquid of box body and interior slotware from flowing out.
Accompanying drawing explanation
Fig. 1 is front view of the present utility model;
Fig. 2 is left view of the present utility model;
Fig. 3 is the vertical view of the utility model cooling box main body;
In figure: 1, cooling box main body, 2, insulation cover, 3, rectangle sample slot, 4, cylindrical sample slot.
Embodiment
Below in conjunction with the drawings and specific embodiments the utility model be described further and limit.
As shown in Figure 1, 2, 3, a kind of Simple multipurpose program temperature reduction box, comprise cooling box main body 1, insulation cover 2 and sample slot, insulation cover 2 is positioned at the top of cooling box main body 1, threaded sealing fit is passed through with cooling box main body 1, sample slot is positioned at the inside of cooling box main body 1, and cooling box main body 1 and sample slot seal and combine.Described sample slot comprises rectangle sample slot 3 and cylindrical sample slot 4.
In the present embodiment, the quantity of described rectangle sample slot 3 is 3, and length is 160mm, and width is 15mm, and the degree of depth is 60mm.3 rectangle sample slot 3 are positioned at the central authorities of cooling box main body 1.Described cylindrical sample slot 4 has two profiles number, and a kind of aperture is 12mm, and the degree of depth in hole is 55mm, and quantity is 10, for depositing the cryopreservation tube of 2ml; Another kind of specification aperture is 12mm, and the degree of depth in hole is 40mm, and quantity is 6, for depositing the cryopreservation tube of 5ml.
In the present embodiment, described cooling box main body 1 is cylindrical, and diameter is 180mm, high 65mm.Described cooling box main body 1, insulation cover 2 and sample slot all use polythene PE material to make.
Simple multipurpose cooling box described in the utility model, compared with existing freezing storing box, increases the purposes of program temperature reduction box.Not only can be used for the frozen a small amount of cell of cryopreservation tube of current regular size, can also, as frozen bag, be the cooling of a large amount of cells of conserving appliance.
Above embodiment is only unrestricted for illustration of the technical solution of the utility model, and under the prerequisite not departing from the utility model design spirit, the various changes that those skilled in the art make in form and details, should belong to protection domain of the present utility model.
Claims (5)
1. a Simple multipurpose program temperature reduction box, comprise cooling box main body, insulation cover and sample slot, insulation cover is positioned at the top of cooling box main body, sample slot is positioned at the inside of cooling box main body, it is characterized in that: described sample slot comprises rectangle sample slot for depositing different model cryopreservation tube and cylindrical sample slot; Described cooling box main body and insulation cover pass through threaded sealing fit.
2. Simple multipurpose program temperature reduction box according to claim 1, is characterized in that: the quantity of described rectangle sample slot is 2-5, and length is 140-180mm, and width is 10-20mm, and the degree of depth is 50-80mm; Described cylindrical sample slot has two profiles number, and a kind of aperture is 10-30mm, and hole depth is 40-60mm, and another kind of aperture is 10-30mm, and hole depth is 30-50mm, and the quantity of cylindrical sample slot is 10-20.
3. Simple multipurpose program temperature reduction box according to claim 2, is characterized in that: described cooling box main body is combined by thread seal with sample slot.
4. Simple multipurpose program temperature reduction box according to claim 3, is characterized in that: described cooling box main body is cylindrical.
5. Simple multipurpose program temperature reduction box according to claim 4, is characterized in that: described cooling box main body, insulation cover and sample slot all use polythene PE material to make.
Priority Applications (1)
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CN201520557367.3U CN204811679U (en) | 2015-07-29 | 2015-07-29 | Simple and easy multipurpose procedure cooling box |
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CN201520557367.3U CN204811679U (en) | 2015-07-29 | 2015-07-29 | Simple and easy multipurpose procedure cooling box |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107087589A (en) * | 2017-07-04 | 2017-08-25 | 刘晓丽 | Suitable for cryopreservation tube and the adjustable freezing storing box of bulk tissue |
CN110495448A (en) * | 2019-09-18 | 2019-11-26 | 英诺维尔智能科技(苏州)有限公司 | One kind freezing a bag priming device |
-
2015
- 2015-07-29 CN CN201520557367.3U patent/CN204811679U/en active Active
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107087589A (en) * | 2017-07-04 | 2017-08-25 | 刘晓丽 | Suitable for cryopreservation tube and the adjustable freezing storing box of bulk tissue |
CN110495448A (en) * | 2019-09-18 | 2019-11-26 | 英诺维尔智能科技(苏州)有限公司 | One kind freezing a bag priming device |
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