CN204811679U - Simple and easy multipurpose procedure cooling box - Google Patents

Abstract

The utility model discloses a simple multi-purpose program cooling box, which comprises a cooling box main body, a heat preservation cover and a sample tank, the heat preservation cover is located above the cooling box main body, the sample tank is located inside the cooling box main body, and the sample tank includes Rectangular sample tanks and cylindrical sample tanks for storing different types of cryopreservation tubes; the main body of the cooling box and the heat preservation cover are sealed and matched by threads. The utility model can not only be used for the simple program cryopreservation of single cells or tissue types, but also meet the needs of cooling storage of larger volume cell suspensions and simultaneous program cooling of various cryopreservation containers. The main body of the cooling box and the insulation cover are thread-tightly matched, which is more conducive to maintaining the stability of the cooling box and preventing the liquid from flowing out of the box body and the inner tank.

Description

A simple multi-purpose programmed cooling box

technical field

The utility model relates to a programmed cooling box, in particular to a simple multi-purpose programmed cooling box.

Background technique

The cryopreservation of cells requires the addition of suitable cryoprotectants to prevent the formation of ice crystals in the cells and in the water in the environment during the cooling process. Failure to add cryoprotectants will lead to a series of changes in cells, such as mechanical damage, increased electrolyte concentration, osmotic pressure changes, dehydration, pH value changes, protein denaturation, etc., causing cell damage or even death.

At present, the most commonly used storage method for cells and tissues is cryopreservation (stored at -196°C), and the cooling process has become the most critical factor in determining the storage effect. At present, the cooling process of deep cryopreservation of single cells mainly adopts a slow cooling method, which can be realized by using a computer-controlled programmed cooling device to ensure the survival of cells. However, due to the disadvantages of high cost, large floor space, cumbersome and complicated operation, and the need for timely supply of liquid nitrogen, most domestic researchers generally use refrigerators to cool down step by step. In recent years, simple programmed cooling boxes have been gradually applied to the freezing of cells and tissues .

The simple program cooling box uses organic reagents such as isopropanol as a buffer, and the cells and the program cooling box that have been balanced at 4°C are placed at -80°C to achieve a cooling rate of 1°C/min, which basically meets the deep cryogenic temperature requirements of cells. Preservation requires slow cooling, and the cells maintain a high cell viability after cryopreservation.

The cryopreservation concentration of cells is generally between 5×10 ⁵ /mL and 1×10 ⁷ /mL. If the concentration of cells is too high or too low, the viability of cells after cryopreservation will be reduced, which will significantly affect the freezing effect. With the development of clinical trials and research, the demand for more cells (even up to 109) increases, and larger volume storage containers are particularly important. Similarly, the program cooling that can meet the corresponding large volume of cell cryopreservation Installations are also imminent. The currently commonly used tube-trough-shaped programmed cooling box is only suitable for specific types of cell cryopreservation tubes, which cannot meet the above requirements.

Contents of the invention

Aiming at the defects of the prior art, the utility model provides a simple multi-purpose program cooling box, which can not only be used for the simple program freezing storage of single cells or tissue types, but also meet the cooling storage of large-volume cell suspensions and various The need for simultaneous programmed cooling of cryopreservation containers.

In order to solve the above technical problems, the technical solution adopted by the utility model is: a simple multi-purpose program cooling box, including the main body of the cooling box, the heat preservation cover and the sample tank, the heat preservation cover is located above the main body of the cooling box, and the sample tank is located in the cooling box Inside the main body, the sample tank includes a rectangular sample tank and a cylindrical sample tank for storing different types of cryopreservation tubes; the main body of the cooling box and the heat preservation cover are tightly matched by threads.

Further, the number of rectangular sample tanks is 2-5, the length is 140-180mm, the width is 10-20mm, and the depth is 50-80mm; the cylindrical sample tank has two types, one with an aperture of 10 -30mm, the hole depth is 40-60mm, another hole diameter is 10-30mm, the hole depth is 30-50mm, and the number of cylindrical sample slots is 10-20.

Further, the main body of the cooling box is combined with the sample tank through thread sealing.

Further, the main body of the cooling box is cylindrical.

Further, the main body of the cooling box, the heat preservation cover and the sample tank are all made of polyethylene PE material.

Beneficial effects of the utility model: the simple multi-purpose program cooling box of the utility model includes a main body of the cooling box, a thermal insulation cover and a sample tank, and the sample tank includes a rectangular sample tank and a cylindrical sample tank, and there are two kinds of cylindrical sample tanks The model can not only be used for simple program cryopreservation of single cells or tissue types, but also meet the needs of cooling storage of large-volume cell suspensions and simultaneous program cooling of various cryopreservation containers. The main body of the cooling box and the insulation cover are thread-tightly matched, which is more conducive to maintaining the stability of the cooling box and preventing the liquid from flowing out of the box body and the inner tank.

Description of drawings

Fig. 1 is the front view of the utility model;

Fig. 2 is the left view of the utility model;

Fig. 3 is a top view of the main body of the utility model cooling box;

In the figure: 1. Main body of cooling box, 2. Insulation cover, 3. Rectangular sample tank, 4. Cylindrical sample tank.

Detailed ways

The utility model will be further described and limited below in conjunction with the accompanying drawings and specific embodiments.

As shown in Figures 1, 2, and 3, a simple multi-purpose program cooling box includes a main body 1 of the cooling box, a heat preservation cover 2 and a sample tank. Sealed fit, the sample tank is located inside the main body 1 of the cooling box, and the main body 1 of the cooling box is sealed and combined with the sample tank. The sample tank includes a rectangular sample tank 3 and a cylindrical sample tank 4 .

In this embodiment, the number of the rectangular sample slots 3 is 3, the length is 160 mm, the width is 15 mm, and the depth is 60 mm. Three rectangular sample tanks 3 are located in the center of the main body 1 of the cooling box. Described cylindrical sample tank 4 has two kinds of models, and a kind of aperture is 12mm, and the depth of hole is 55mm, and quantity is 10, is used for depositing the cryopreservation tube of 2ml; Another kind of specification aperture is 12mm, and the depth of hole is 40mm, the quantity is 6, used to store 5ml cryovials.

In this embodiment, the main body 1 of the cooling box is cylindrical, with a diameter of 180 mm and a height of 65 mm. The main body 1 of the cooling box, the heat preservation cover 2 and the sample tank are all made of polyethylene PE material.

Compared with the existing freeze storage box, the simple and multipurpose cooling box described in the utility model has more applications of the programmed cooling box. Not only can it be used to freeze a small amount of cells in cryopreservation tubes of commonly used specifications, but it can also be used as a cryopreservation bag to cool down a large number of cells in preservation tools.

The above embodiments are only used to illustrate the technical solutions of the present utility model and are not limiting. Under the premise of not departing from the design spirit of the present utility model, various changes made by those skilled in the art in form and details should belong to the utility model scope of protection.

Claims (5)

1. a Simple multipurpose program temperature reduction box, comprise cooling box main body, insulation cover and sample slot, insulation cover is positioned at the top of cooling box main body, sample slot is positioned at the inside of cooling box main body, it is characterized in that: described sample slot comprises rectangle sample slot for depositing different model cryopreservation tube and cylindrical sample slot; Described cooling box main body and insulation cover pass through threaded sealing fit.

2. Simple multipurpose program temperature reduction box according to claim 1, is characterized in that: the quantity of described rectangle sample slot is 2-5, and length is 140-180mm, and width is 10-20mm, and the degree of depth is 50-80mm; Described cylindrical sample slot has two profiles number, and a kind of aperture is 10-30mm, and hole depth is 40-60mm, and another kind of aperture is 10-30mm, and hole depth is 30-50mm, and the quantity of cylindrical sample slot is 10-20.

3. Simple multipurpose program temperature reduction box according to claim 2, is characterized in that: described cooling box main body is combined by thread seal with sample slot.

4. Simple multipurpose program temperature reduction box according to claim 3, is characterized in that: described cooling box main body is cylindrical.

5. Simple multipurpose program temperature reduction box according to claim 4, is characterized in that: described cooling box main body, insulation cover and sample slot all use polythene PE material to make.