CN205250211U - Can monitor procedure cooling box of cell freezing process - Google Patents
Can monitor procedure cooling box of cell freezing process Download PDFInfo
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- CN205250211U CN205250211U CN201521087668.0U CN201521087668U CN205250211U CN 205250211 U CN205250211 U CN 205250211U CN 201521087668 U CN201521087668 U CN 201521087668U CN 205250211 U CN205250211 U CN 205250211U
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- temperature reduction
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- reduction box
- program temperature
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Abstract
The utility model discloses a can monitor procedure cooling box of cell freezing process, including procedure cooling box box body and with a procedure cooling box box body matched with lid to and place the freezing cooling box in procedure cooling box box body, freezing cooling box is including freezing cooling box box body and setting up the mould on the freezing box box body of cooling down, be provided with the sample groove that is used for depositing the sample on the mould, form the sealed cavity that is used for placing cryoprotectant between freezing cooling box box body and the mould, be provided with on the freezing cooling box with the cryoprotectant application of sample pipe of sealed cavity intercommunication, one side of procedure cooling box box body is provided with the control box, be provided with temperature sensor, controller and data memory on the control box. The utility model discloses a procedure cooling box simple structure, reasonable in design can realize the control to the cell freezing process, guarantees the safety and the stability of cell freezing process.
Description
Technical field
The utility model belongs to medical science Refrigeration Technique field, is specifically related to one and can monitors cell freezing mistakeThe program temperature reduction box of journey.
Background technology
Cell cryopreservation is under the low temperature environment that cell is preserved, and makes its metabolism substantially in suspending, therebyEnsure that the every biological nature of cell is complete after long-term preservation, in order to using from now on. And cell freezing mistakeJourney is to carry out frozen steps necessary, and the quality of refrigerating effect directly affects the biology of Cell CryopreservationActivity and biological characteristics, play very important effect to the cell preservation process for a long time of carrying out.
Conventional freezing method mainly contains two kinds at present: the cooling of a kind of employing thermograde will be treated freeze-stored cellProgressively be placed in 4 DEG C of 40min ,-20 DEG C of 1h ,-80 DEG C spend the night, be then transferred to liquid nitrogen and preserve for a long time; SeparatelyOne is cell to be put into programmed cooling instrument realize and put into liquid nitrogen after gradient cooling again and preserve for a long time. SoAnd the former complex operation, refrigerating process is not controlled, has the otherness of manual operation. The latter's program is fallenTemperature instrument is expensive, and liquid nitrogen consumption is large, operates not easyly, and the little words cost of sample throughputHigh.
There is program temperature reduction box for front kind of method at present, the cell of cryopreservation tube dress can have been put into journeyIn order cooling box, at the bottom of box, by adding isopropyl alcohol, then whole program temperature reduction box is put into-80 DEG C of iceCase, thus reach the cooling of 1 DEG C of left and right per minute. But this program temperature reduction box exists isopropyl alcohol repeatedly(isopropyl alcohol has toxicity, volatility, combustibility and easily absorbs moisture, due to water suction meeting in replacingCause its freezing point to change, thus need regularly repeatedly to add isopropyl alcohol, to ensure temperature-fall period),Actual temperature-fall period is not controlled, be only applicable to indivedual cells carries out frozen preservation.
Utility model content
Technical problem to be solved in the utility model is, for above-mentioned the deficiencies in the prior art, to provideA kind of program temperature reduction box of monitoring cell freezing process. This program temperature reduction box is simple in structure, and design is closedReason, can realize the monitoring to cell freezing process, ensures safety and the stability of cell freezing process.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is: one can be monitored carefullyThe program temperature reduction box of born of the same parents' refrigerating process, is characterized in that, comprises program temperature reduction box box body and falls with programThe lid that temperature box box body matches, and be positioned over the frozen cooling box in program temperature reduction box box body, instituteStating frozen cooling box comprises frozen cooling box box body and is arranged at the mould on frozen cooling box box body, instituteState on mould and be provided with the sample slot for depositing sample, between described frozen cooling box box body and mouldBe formed for placing the sealed cavity of cryoprotector, on described frozen cooling box, be provided with described closeThe cryoprotector application of sample pipe that envelope cavity is communicated with, on described cryoprotector application of sample pipe and be positioned at freezing fallingThe one end in temperature box outside is provided with dismountable closeouts; One side setting of described program temperature reduction box box bodyThere is monitor cartridge, in described monitor cartridge, be provided with temperature sensor, controller and join with controller and useIn the data storage of stored record data, input and the temperature sensor of described controller join.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described prisonThe display floater of the temperature that is provided with alarm on control box and detect for displays temperature sensor, described inAlarm is all connected with the output of controller with display floater.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described reportAlert device is audible-visual annunciator.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described journeyOrder cooling box box body is stainless steel box body, and lid is stainless steel lid.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described temperatureOne side of degree sensor setting close program temperature reduction box box body in monitor cartridge, and the survey of temperature sensorTemperature probe is positioned at program temperature reduction box box body.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described temperatureThe quantity of degree sensor is multiple.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that, described coldFreezing cooling box box body and mould is integral type structure.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described sampleThis groove is cuboid sample slot or cylindrical sample slot.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that, described coldFreeze cooling box box body arranged outside and have volume markings line.
Above-mentioned a kind of program temperature reduction box of monitoring cell freezing process, is characterized in that described envelopeBlocking piece is pipe lid or pipe close.
The utility model compared with prior art has the following advantages:
1, the utility model is simple in structure, reasonable in design, can realize the monitoring to cell freezing process,Ensure safety and the stability of cell freezing process.
2, the utility model, by frozen cooling box is set, arranges cryoprotection on frozen cooling boxAgent application of sample pipe, and on cryoprotector application of sample pipe, closeouts is set, freezing to adding in sealed cavityAfter protective agent, make cryoprotector in sealed environment with closeouts shutoff cryoprotector application of sample pipe,Interpolation repeatedly and the pollution to environment of cryoprotector are avoided.
3, the utility model, by the design of the mould to frozen cooling box, can adapt to differenceCryopreservation tube and freezer bag.
4, the utility model is by set temperature sensor, controller and data storage, Neng GoushiThe now Real-Time Monitoring to cell freezing process temperature and storage, so that the record to refrigerating process and chasing afterTrace back.
5, the utility model, by alarm is set, can be realized the report to refrigerating process anomalyAlert, the reliability of guarantee refrigerating process.
6, the utility model, by cryoprotector application of sample pipe is set, can add not in sealed cavitySame volume and different types of reagent, can realize different cooling rates, meets various cell freezing needWant.
Below in conjunction with drawings and Examples, the technical solution of the utility model is done further to say in detailBright.
Brief description of the drawings
Fig. 1 is the program temperature reduction box that the utility model the first detailed description of the invention is removed frozen cooling boxDecomposing schematic representation.
Fig. 2 is the structural representation of the frozen cooling box of the utility model the first detailed description of the invention.
Fig. 3 is the A-A cutaway view of Fig. 2.
Fig. 4 is the schematic block circuit diagram of the utility model monitor cartridge.
Fig. 5 is the program temperature reduction box that the utility model the second detailed description of the invention is removed frozen cooling boxDecomposing schematic representation.
Fig. 6 is the structural representation of the frozen cooling box of the utility model the second detailed description of the invention.
Fig. 7 is the B-B cutaway view of Fig. 6.
Description of reference numerals:
1-program temperature reduction box box body; 2-lid; 3-frozen cooling box box body;
4-mould; 5-sample slot; 6-monitor cartridge;
7-sealed cavity; 8-cryoprotector application of sample pipe; 9-closeouts;
10-controller; 11-data storage; 12-temperature sensor;
13-alarm; 14-display floater.
Detailed description of the invention
Embodiment 1
As shown in Figure 1, Figure 2, Figure 3 and Figure 4, the cell freezing monitored of the present embodiment processProgram temperature reduction box, the lid that comprises program temperature reduction box box body 1 and match with program temperature reduction box box body 12, and be positioned over the frozen cooling box in program temperature reduction box box body 1, described frozen cooling box comprisesFrozen cooling box box body 3 and be arranged at the mould 4 on frozen cooling box box body 3, on described mould 4Be provided with the sample slot 5 for depositing sample, shape between described frozen cooling box box body 3 and mould 4Become the sealed cavity 7 for placing cryoprotector, on described frozen cooling box, be provided with described closeThe cryoprotector application of sample pipe 8 that is communicated with of envelope cavity 7, on described cryoprotector application of sample pipe 8 and be positioned atThe one end in frozen cooling box outside is provided with dismountable closeouts 9; Described program temperature reduction box box body 1A side be provided with monitor cartridge 6, in described monitor cartridge 6, be provided with temperature sensor 12, controller 10With join with controller 10 and for the data storage 11 of stored record data, described controller 10Input and temperature sensor 12 join.
In the present embodiment, in described monitor cartridge 6, be provided with alarm 13 and for displays temperature sensingThe display floater 14 of the temperature that device 12 detects, described alarm 13 and display floater 14 all with controlThe output of device 10 connects.
In the present embodiment, described alarm 13 is audible-visual annunciator.
In the present embodiment, described program temperature reduction box box body 1 is stainless steel box body, and lid 2 is stainless steelLid.
In the present embodiment, described temperature sensor 12 is arranged in monitor cartridge 6 near program temperature reduction boxOne side of box body 1, and the temperature probe of temperature sensor 12 is positioned at program temperature reduction box box body 1.
In the present embodiment, the quantity of described temperature sensor 12 is 4.
In the present embodiment, described frozen cooling box box body 3 and mould 4 are integral type structure.
In the present embodiment, described sample slot 5 is cylindrical sample slot.
In the present embodiment, described frozen cooling box box body 3 arranged outside have volume markings line.
In the present embodiment, described closeouts 9 is pipe lid or pipe close.
The using method of the program temperature reduction box of the cell freezing the monitored process of the present embodiment is: by coldFreeze protective agent application of sample pipe 8 adds cryoprotector (as isopropyl in the sealed cavity 7 of frozen cooling boxAlcohol), with the mouth of pipe of closeouts 9 shutoff cryoprotector application of sample pipes 8, by 4, standard items are housedCryopreservation tube and several being equipped with need the cryopreservation tube of frozen cell to put into sample slot 5, then by frozen coolingBox is placed in program temperature reduction box box body 1 cryopreservation tube is vertically placed, by 4 temperature sensors 12Temperature probe stretches into respectively in the cryopreservation tube that standard items are housed, and builds lid 2, and program temperature reduction box is putIn ultra low temperature freezer, carry out frozen cooling, frozen cooling process middle controller 10 is by temperature sensor12 temperature that detect show by display floater 14, and the temperature that temperature sensor 12 is detectedCompare with preset alarm threshold temperature, exceed alarm, thereby realize the prison to frozen cooling processSurvey and report to the police.
Embodiment 2
As shown in Figure 4, Figure 5, Figure 6 and Figure 7, the cell freezing monitored of the present embodiment processProgram temperature reduction box, the lid that comprises program temperature reduction box box body 1 and match with program temperature reduction box box body 12, and be positioned over the frozen cooling box in program temperature reduction box box body 1, described frozen cooling box comprisesFrozen cooling box box body 3 and be arranged at the mould 4 on frozen cooling box box body 3, on described mould 4Be provided with the sample slot 5 for depositing sample, shape between described frozen cooling box box body 3 and mould 4Become the sealed cavity 7 for placing cryoprotector, on described frozen cooling box, be provided with described closeThe cryoprotector application of sample pipe 8 that is communicated with of envelope cavity 7, on described cryoprotector application of sample pipe 8 and be positioned atThe one end in frozen cooling box outside is provided with dismountable closeouts 9; Described program temperature reduction box box body 1A side be provided with monitor cartridge 6, in described monitor cartridge 6, be provided with temperature sensor 12, controller 10With join with controller 10 and for the data storage 11 of stored record data, described controller 10Input and temperature sensor 12 join.
In the present embodiment, in described monitor cartridge 6, be provided with alarm 13 and for displays temperature sensingThe display floater 14 of the temperature that device 12 detects, described alarm 13 and display floater 14 all with controlThe output of device 10 connects.
In the present embodiment, described alarm 13 is audible-visual annunciator.
In the present embodiment, described program temperature reduction box box body 1 is stainless steel box body, and lid 2 is stainless steelLid.
In the present embodiment, described temperature sensor 12 is arranged in monitor cartridge 6 near program temperature reduction boxOne side of box body 1, and the temperature probe of temperature sensor 12 is positioned at program temperature reduction box box body 1.
In the present embodiment, the quantity of described temperature sensor 12 is 1.
In the present embodiment, described frozen cooling box box body 3 and mould 4 are integral type structure.
In the present embodiment, described sample slot 5 is cuboid sample slot.
In the present embodiment, described frozen cooling box box body 3 arranged outside have volume markings line.
In the present embodiment, described closeouts 9 is pipe lid or pipe close.
The using method of the program temperature reduction box of the cell freezing the monitored process of the present embodiment is: by coldFreeze protective agent application of sample pipe 8 adds cryoprotector (as isopropyl in the sealed cavity 7 of frozen cooling boxAlcohol), with the mouth of pipe of closeouts 9 shutoff cryoprotector application of sample pipes 8, by 1, standard items are housedFreezer bag and several being equipped with need the freezer bag of frozen cell to put into sample slot 5, then by frozen coolingBox is placed in program temperature reduction box box body 1 and makes freezer bag horizontal positioned, by the survey of temperature sensor 12Temperature probe stretches in the freezer bag that standard items are housed, and builds lid 2, program temperature reduction box is placed in ultralowIn temperature refrigerator, carry out frozen cooling, frozen cooling process middle controller 10 detects temperature sensor 12To temperature show by display floater 14, and the temperature that temperature sensor 12 is detected is with defaultAlarm threshold value temperature compares, and exceedes alarm, thereby realizes monitoring and report to frozen cooling processAlert.
The above, be only preferred embodiment of the present utility model, not the utility model done anyRestriction, every any simple modification of above embodiment being done according to the utility model technical spirit,Change and equivalent structure variation, all still belong in the protection domain of technical solutions of the utility model.
Claims (10)
1. the program temperature reduction box that can monitor cell freezing process, is characterized in that, comprises programCooling box box body (1) and the lid (2) matching with program temperature reduction box box body (1), Yi JifangBe placed in the frozen cooling box in program temperature reduction box box body (1), described frozen cooling box comprises freezing fallingTemperature box box body (3) and be arranged at the mould (4) on frozen cooling box box body (3), described mould(4) on, be provided with the sample slot (5) for depositing sample, described frozen cooling box box body (3)And between mould (4), be formed for placing the sealed cavity (7) of cryoprotector, described freezing fallingOn temperature box, be provided with the cryoprotector application of sample pipe (8) being communicated with described sealed cavity (7), described inCryoprotector application of sample pipe (8) one end upper and that be positioned at frozen cooling box outside is provided with dismountableCloseouts (9); One side of described program temperature reduction box box body (1) is provided with monitor cartridge (6), instituteState in monitor cartridge (6), be provided with temperature sensor (12), controller (10) and with controller (10)Join and for the data storage (11) of stored record data, the input of described controller (10)End joins with temperature sensor (12).
2. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, in described monitor cartridge (6), be provided with alarm (13) and pass for displays temperatureThe display floater (14) of the temperature that sensor (12) detects, described alarm (13) and display floater(14) be all connected with the output of controller (10).
3. a kind of program temperature reduction box of monitoring cell freezing process according to claim 2,It is characterized in that, described alarm (13) is audible-visual annunciator.
4. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, described program temperature reduction box box body (1) is stainless steel box body, and lid (2) is stainlessSteel lid.
5. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, it is upper near programmed cooling that described temperature sensor (12) is arranged at monitor cartridge (6)One side of box box body (1), and the temperature probe of temperature sensor (12) is positioned at program temperature reduction box boxIn body (1).
6. a kind of program temperature reduction box of monitoring cell freezing process according to claim 5,It is characterized in that, the quantity of described temperature sensor (12) is multiple.
7. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, described frozen cooling box box body (3) and mould (4) are integral type structure.
8. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, described sample slot (5) is cuboid sample slot or cylindrical sample slot.
9. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, described frozen cooling box box body (3) arranged outside has volume markings line.
10. a kind of program temperature reduction box of monitoring cell freezing process according to claim 1,It is characterized in that, described closeouts (9) is pipe lid or pipe close.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521087668.0U CN205250211U (en) | 2015-12-24 | 2015-12-24 | Can monitor procedure cooling box of cell freezing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201521087668.0U CN205250211U (en) | 2015-12-24 | 2015-12-24 | Can monitor procedure cooling box of cell freezing process |
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| Publication Number | Publication Date |
|---|---|
| CN205250211U true CN205250211U (en) | 2016-05-25 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201521087668.0U Active CN205250211U (en) | 2015-12-24 | 2015-12-24 | Can monitor procedure cooling box of cell freezing process |
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| CN (1) | CN205250211U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107318827A (en) * | 2017-07-03 | 2017-11-07 | 上海理工大学 | A kind of medical speed governing heat sink with communication function |
| CN109164845A (en) * | 2018-09-11 | 2019-01-08 | 希瑞干细胞科技有限公司 | Band can write the programmed cooling instrument of cooling process |
| CN110476955A (en) * | 2019-09-16 | 2019-11-22 | 上海理工大学 | A kind of programmed cell plant ice production apparatus |
| CN113142193A (en) * | 2021-05-27 | 2021-07-23 | 上海理工大学 | Cell program freezing method, system, equipment, medium and device |
| CN115226705A (en) * | 2022-06-20 | 2022-10-25 | 浙江师范大学 | Cell cryopreservation and resuscitation integrated equipment |
-
2015
- 2015-12-24 CN CN201521087668.0U patent/CN205250211U/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107318827A (en) * | 2017-07-03 | 2017-11-07 | 上海理工大学 | A kind of medical speed governing heat sink with communication function |
| CN109164845A (en) * | 2018-09-11 | 2019-01-08 | 希瑞干细胞科技有限公司 | Band can write the programmed cooling instrument of cooling process |
| CN110476955A (en) * | 2019-09-16 | 2019-11-22 | 上海理工大学 | A kind of programmed cell plant ice production apparatus |
| CN110476955B (en) * | 2019-09-16 | 2021-07-20 | 上海理工大学 | Cell procedure ice planting device |
| CN113142193A (en) * | 2021-05-27 | 2021-07-23 | 上海理工大学 | Cell program freezing method, system, equipment, medium and device |
| CN113142193B (en) * | 2021-05-27 | 2024-01-30 | 上海理工大学 | Cell program freezing method, system, equipment, medium and device |
| CN115226705A (en) * | 2022-06-20 | 2022-10-25 | 浙江师范大学 | Cell cryopreservation and resuscitation integrated equipment |
| CN115226705B (en) * | 2022-06-20 | 2024-04-19 | 浙江师范大学 | Cell cryopreservation and resuscitation integrated equipment |
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