CN206963793U - The heat sink used during stem cell cryopreserving - Google Patents

The heat sink used during stem cell cryopreserving Download PDF

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Publication number
CN206963793U
CN206963793U CN201720452653.2U CN201720452653U CN206963793U CN 206963793 U CN206963793 U CN 206963793U CN 201720452653 U CN201720452653 U CN 201720452653U CN 206963793 U CN206963793 U CN 206963793U
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stem cell
heat sink
cell cryopreserving
interior bucket
sink used
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CN201720452653.2U
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朱业峰
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BOYA STEM CELL TECHNOLOGY Co Ltd
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BOYA STEM CELL TECHNOLOGY Co Ltd
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Abstract

The application is related to the heat sink used during stem cell cryopreserving.Specifically, the application is related to the heat sink used during a kind of stem cell/immunocyte freezes, and it includes:Staving, it is sandwich design, including bottom is each the outer barrel and inner barr of sealing, outer barrel is higher than interior bucket, annular top cover is formed along concordant and sealing therebetween, the open top of interior bucket, the interlayer of sealing is thus formed between outer barrel and interior bucket in outer barrel and inner barr;Sample adapter, it is a circular slab, the outer of the circular slab matches with the open top of the interior bucket, one or more openings that can be matched with stem cell cryopreserving container are provided with the circular slab, so that stem cell cryopreserving container inner chamber of the interior bucket after the opening is inserted forms relatively closed space.The application heat sink has advantage as used in the description.

Description

The heat sink used during stem cell cryopreserving
Technical field
The application is related to a kind of heat sink, is filled more particularly to a kind of cooling used during stem cell cryopreserving Put, more particularly to one kind is stem cell is cooled by predetermined way and in this temperature-fall period during stem cell cryopreserving In facilitate stem cell to be mixed with frozen stock solution, a kind of such as heat sink of hand mix.During the stem cell cryopreserving of the application Disposal of the heat sink used particularly suitable for stem cell during freezing.
Background technology
Generally, stem cell makes stem cell be mixed with frozen stock solution, it is necessary to add frozen stock solution after collection, processing It is even, then preserved at a temperature of being placed into ultralow temperature such as -80 DEG C of even lower -196 DEG C.And stem cell is cold from room temperature But into the temperature course of this ultralow temperature, it is necessary to slowly cool during carry out mixed processing, make stem cell and frozen stock solution It is well mixed while cooling.
Stem cell sample is adding frozen stock solution during this at present, substantially using traditional ice bag parcel centrifuge tube (or directly centrifuge tube being put into the box equipped with isopropanol, body directly contacts with isopropanol), so as to indirectly to sample This progress precooling while the holding sample relative low temperature during sample adds frozen stock solution;Exist in above procedure ice bag to from Heart pipe parcel is not bonded and ice bag heating is very fast (isopropanol easily splashes, and causes the pollution of environment or sample), and is not easy to carry out One hand mixes operation.
Prior art not publicly can solve the problem that the method or scheme of above-mentioned technical problem.Freeze in spite of some other stem cell The middle device used is deposited, but they are not all suitable for the above-mentioned application field of the application, solve above-mentioned technical difficulty.For example, CN 205124851U (201520942099.7) discloses a kind of cell freezing storage device, including freezes ice chest body, freezes ice chest body and adopts With sealing structure, the inner homogeneous for freezing ice chest body is provided with carrier ring, and hollow part is provided with cell between adjacent separator layer Accommodating area, each cell accommodating area are mounted on the cabinet door being flexibly opened and closed, temperature controller, temperature control are provided with below cell accommodating area Device connects control panel by temperature sensor, and control panel is internally provided with electric-controlled plate linking refrigerator, the outlet of refrigerator Hollow region in mouth linking freezer box, is freezed, each cell accommodating area so that cell is placed in casing at any time A corresponding cabinet door, do not influence the temperature of other cells when placing, make stem cell can be with uniform decrease in temperature, it is believed that square law device is grasped Make simple, time-consuming shorter, it is ensured that the activity of cell, the demand of social development is adapted to, it is novel in design, it is a kind of innovation well Scheme, there is very much market promotion prospect.However, regrettably, these above-mentioned designs not can effectively solve the problem that this Shen Please above-mentioned the problem of referring to.
Therefore, this area is still expected to have new design to solve the above-mentioned technical problem run into of the application, especially Be that by stem cell realized in freezing temperature-fall period it is convenient in cooling while the requirement of sample treatment that mixes, such as gram Take the sample temperature that stem cell sample occurs in frozen stock solution this link is added easily rise, heat sink to centrifuge tube (or Other carriers) inclusion has infelicity, and one hand, which is not allowed to be easy to do, mixes the technical barrier of operation.
Utility model content
The purpose of this utility model is the design for providing the heat sink used during a kind of new stem cell cryopreserving, Expect it is such a/such it is new be designed to solve the above-mentioned technical problem run into of the application, be especially that by stem cell and exist The requirement that the convenient sample treatment mixed in cooling is realized in temperature-fall period is frozen, such as overcomes stem cell sample adding The sample temperature occurred in this link of frozen stock solution easily rises, heat sink does not paste to centrifuge tube (or other carriers) inclusion Cut, one hand, which is not allowed to be easy to do, mixes the technical barrier of operation.It has been had now surprisingly been found that, by the application, design can be with Readily meet these particular/special requirements and solve these special technical problems, the application is it is thus found that and be accomplished.
Therefore, the first aspect of the application provides the heat sink used during a kind of stem cell cryopreserving, its feature It is, it includes:
Staving, it is sandwich design, including bottom is each the outer barrel and inner barr of sealing, and outer barrel is higher than interior bucket, outer barrel Form annular top cover along concordant and sealing therebetween with interior bucket, the open top of interior bucket, thus outer barrel and interior bucket it Between form the interlayer of sealing;
Sample adapter, it is a circular slab, and the outer of the circular slab matches with the open top of the interior bucket, the circle One or more openings that can be matched with stem cell cryopreserving container are provided with plate, so that stem cell cryopreserving container The inner chamber of the interior bucket forms relatively closed space after the opening is inserted.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, refrigerant hand-hole and the stopple for sealing the refrigerant hand-hole is additionally provided with the annular top cover of the staving. Refrigerant can be sealed storage in interlayer in the case of not having the design of refrigerant hand-hole;Also can be existed by the refrigerant hand-hole Add when needs, taken out unwanted when or when replacing.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, refrigerant hand-hole and the stopple for sealing the refrigerant hand-hole, institute is additionally provided with the annular top cover of the staving Stopple is stated to be connected in annular top cover by chain rope.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, the sample adapter is less than the depth of interior bucket configuring its height after sample is frozen.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, the opening on the sample adapter circular slab is multiple circles, is matched for the top with centrifuge tube.In order to as far as possible The more stem cell samples of processing, arranged on sample adapter circular slab it is as much as possible opening be preferable;Now, when need It smaller centrifuge tube can be used to dispense when stem cell samples are split into smaller deal, such as use 15ml centrifuge tubes point Dress, can now arrange more openings on circular slab;And can when needing stem cell samples splitting into bigger deal Dispensed using bigger centrifuge tube, such as dispensed using 50ml centrifuge tubes, slightly few opening can be now arranged on circular slab.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, the opening on the sample adapter circular slab is one square, for being matched with freezing bag.Now, in order to more convenient Matched with freezing bag, the opening being square its it is down-set be in a square groove, make to freeze inner chamber middle position of the bag in interior bucket Put and be relatively fixed.So, what is taken the shape of a bag freezes bag after it with the addition of the stem cell mixed with frozen stock solution, is directly inserted into the party In shape opening;Or the bag that freezes taken the shape of a bag can be also used for preserving frozen stock solution, for when adding frozen stock solution into stem cell Source as the frozen stock solution pre-cooled.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, a thermometer hole for being used to configure device for detecting temperature is also set up on the sample adapter circular slab.Such as it can lead to Cross the thermometer hole device for detecting temperature is placed in the interior bucket below sample adapter, for side in cooling processing procedure in sample Just the temperature regime of monitoring sample local environment.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, the refrigerant being placed in the interlayer is isopropanol.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, it is to be made of clear material that it, which is integrally particularly its staving and sample adapter,.The application is prepared using transparent material to do The heat sink used during cell cryopreservation, addition for refrigerant, to freeze sample troubled water etc. be more convenient observation 's.
According to the heat sink used during the stem cell cryopreserving of the application first aspect any embodiment, its feature It is, reinforcement is additionally provided with the interlayer between the bottom of the interior bucket and outer barrel, for strengthens the knot between interior outer barrel Structure intensity.
In this application, term " refrigerant " refers to the medium of cooling, such as isopropanol, and it absorbs heat after cryogenic freezing Slowly, temperature rises slow, can be maintained at lower temperature with the long period.
Any technical characteristic is equally applicable possessed by any embodiment of the application either side or the either side Any embodiment of other any embodiments or other either sides, as long as they will not be conflicting, certainly mutual Between where applicable, if necessary can individual features be made with appropriate modification.Various aspects of the present utility model and feature are made below Further description.
All documents recited in the application, their full content are incorporated herein by reference, and if these are literary When offering expressed implication and inconsistent the application, it is defined by the statement of the application.In addition, various terms used in this application and Phrase has well known to a person skilled in the art general sense, nonetheless, the application remain desirable at this to these terms and Phrase is described in more detail and explains, the term and phrase referred to is if any inconsistent with common art-recognized meanings, with the application institute table The implication stated is defined.
Brief description of the drawings
Fig. 1 is showing for the assembled state of an embodiment of the heat sink used during the application stem cell cryopreserving It is intended to.
Fig. 2 is two exemplary implementations of the sample adapter of the heat sink used during the application stem cell cryopreserving Scheme.
Embodiment
The utility model can be further described by the following examples, however, model of the present utility model Enclose and be not limited to following embodiments.One of skill in the art is it is understood that without departing substantially from spirit and scope of the present utility model On the premise of, various change and modification can be carried out to the utility model.The utility model is to the material that is arrived used in experiment And test method carries out general and/or specific description.Although to realize many materials used in the utility model purpose Material and operating method are it is known in the art that still the utility model is still described in detail as far as possible herein.
Various embodiments of the present utility model are described in detail with reference to the accompanying drawings.
Fig. 1 is showing for the assembled state of an embodiment of the heat sink used during the application stem cell cryopreserving It is intended to.
Fig. 2 is two exemplary realities of the sample adapter 2 of the heat sink used during the application stem cell cryopreserving Apply scheme.
As shown in figure 1, the heat sink used during the stem cell cryopreserving includes:
Staving 1, it is sandwich design, including bottom is each the outer barrel 11 and interior bucket 12 of sealing, and outer barrel 11 compares interior bucket 12 is high, and outer barrel 11 forms annular top cover 13 with interior bucket 12 along concordant and sealing therebetween, the open top of interior bucket 12, by This forms the interlayer of sealing between outer barrel 11 and interior bucket 12;
Sample adapter 2, it is a circular slab 21, the outer of the circular slab 21 and the open top of the interior bucket 12 Match somebody with somebody, one or more openings 22 that can be matched with stem cell cryopreserving container are provided with the circular slab 21, so that Stem cell cryopreserving container inner chamber of the interior bucket 12 after the opening 22 is inserted forms relatively closed space.
In one embodiment of the heat sink used during the application stem cell cryopreserving, the ring of the staving 1 Refrigerant hand-hole 14 and the stopple 15 for sealing the refrigerant hand-hole 14 are additionally provided with shape top cover 13.Noted in no refrigerant It can be sealed storage in interlayer to enter refrigerant in the case that hole 14 is designed;Also can be by the refrigerant hand-hole 14 in needs When add, taken out unwanted when or when replacing.
In one embodiment of the heat sink used during the application stem cell cryopreserving, the ring of the staving 1 Refrigerant hand-hole 14 and the stopple 15 for sealing the refrigerant hand-hole 14 are additionally provided with shape top cover 13, the stopple 15 is logical Chain rope 16 is crossed to be connected in annular top cover 13.
In one embodiment of the heat sink used during the application stem cell cryopreserving, the sample adapter 2 are less than the depth of interior bucket 12 configuring its height after sample is frozen.
In one embodiment of the heat sink used during the application stem cell cryopreserving, the sample adapter Opening 22 on 2 circular slabs 21 is multiple circular (as shown in Fig. 1 and Fig. 2 A), is matched for the top with centrifuge tube.In order to More stem cell samples are handled as far as possible, and it is preferred that opening 22 as much as possible is arranged on the circular slab 21 of sample adapter 2 's;Now, smaller centrifuge tube can be used to dispense when needing stem cell samples splitting into smaller deal, such as using 15ml centrifuge tubes are dispensed, and more openings 22 can be now arranged on circular slab 21;And working as needs to split stem cell samples Bigger centrifuge tube packing can be used during Cheng Geng great deal, such as dispensed using 50ml centrifuge tubes, now on circular slab 21 Slightly few opening 22 can be arranged.
In one embodiment of the heat sink used during the application stem cell cryopreserving, the sample adapter Opening 22 on 2 circular slabs 21 is one square (as shown in Fig. 2 B), for being matched with freezing bag.Now, in order to more convenient Matched with freezing bag, the opening 22 being square its it is down-set be in a square groove, make to freeze bag in the inner chamber of interior bucket 12 Middle position is relatively fixed.So, what is taken the shape of a bag freezes bag after it with the addition of the stem cell mixed with frozen stock solution, is directly inserted into In the square aperture 22;Or the bag that freezes taken the shape of a bag can be also used for preserving frozen stock solution, for adding jelly into stem cell Source during liquid storage as the frozen stock solution pre-cooled.
In one embodiment of the heat sink used during the application stem cell cryopreserving, the sample adapter A thermometer hole 23 for being used to configure device for detecting temperature is also set up on 2 circular slabs 21.Such as it can be incited somebody to action by the thermometer hole 23 Device for detecting temperature 24 is placed in the interior bucket 12 of the lower section of sample adapter 2, for easily being supervised in cooling processing procedure in sample The temperature regime of test sample product local environment.
In one embodiment of the heat sink used during the application stem cell cryopreserving, it is placed in the interlayer The refrigerant be isopropanol.
In one embodiment of the heat sink used during the application stem cell cryopreserving, its overall particularly bucket Body and sample adapter are to be made of clear material.The drop used during the application stem cell cryopreserving is prepared using transparent material Warm device, addition for refrigerant, to freeze sample troubled water etc. be more convenient observation.
In one embodiment of the heat sink used during the application stem cell cryopreserving, in the interior bucket 12 Reinforcement 25 is additionally provided with interlayer between bottom and outer barrel 11, for strengthening the structural strength between interior outer barrel.
Describe the occupation mode of the heat sink used during the application stem cell cryopreserving with reference to Fig. 1.By refrigerant It is injected into interlayer from refrigerant hand-hole 14 and is sealed with stopple 15, (sample adapter 2 is installed or do not installed) will contains The staving 1 for having refrigerant, which is placed in household freezer, freezes refrigerant therein, when using the heat sink, will be sub-packed in centrifuge tube Stem cell (or with stem cell born of the same parents frozen stock solution dilute stem cell) insertion sample adapter 2 opening 22 in, be adapted to sample Device 2 and staving 1 coordinate that (or sample adapter 2 coordinates and installs and freeze in advance in advance with staving 1, then to sample adapter 2 The centrifuge tube for having dispensed stem cell is placed in opening 22), optional insertion device for detecting temperature 24, thus, it is possible to make stem cell Slowly cool in the application heat sink, and singlehanded whole heat sink can be rocked in temperature-fall period so that Stem cell is well mixed in temperature-fall period.
Or the heat sink of the application is a cylindrical transparent device, wherein there is interlayer, isopropanol is added in interlayer (it is enclosed workshop between interlayer and cylinder, so there can be isopropanol during rocking with avoiding device as temperature-reducing medium Overflow;Isopropanol is imported by cylindrical wall well, after importing, is closed with plug), set among cylindrical appliance Single or multiple apertures are put, its diameter is consistent with 50ml centrifuge tubes, 15ml centrifuge tubes, and its height is slightly larger than centrifuge tube height; Usually the device is placed in cold-storage in 4 degree of refrigerators, takes out during use, after 75% alcohol disinfecting device, is put into Biohazard Safety Equipment, The final centrifuge tube equipped with sample suspensions is put into the device and cooled, frozen stock solution is uniformly added by rocking the device Enter, while can observe frozen stock solution by device tube wall and add situation and whether need to add isopropanol.In addition, the device One rectangular recess can also be set in centre, place candidate stem cell/immunocyte and freeze bag, for candidate stem cell/exempt from Epidemic disease cell adds the process of frozen stock solution.
In description of the present utility model, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width Degree ", " thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outer ", " suitable The orientation or position relationship of the instruction such as hour hands ", " counterclockwise ", " axial direction ", " radial direction ", " circumference " are based on orientation shown in the drawings Or position relationship, be for only for ease of description the utility model and simplify and describe, rather than instruction or imply signified device or Element must have specific orientation, with specific azimuth configuration and operation, therefore it is not intended that to limit of the present utility model System.
In addition, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or Implicitly include one or more this feature.In description of the present utility model, " multiple " are meant that two or two More than, unless otherwise specifically defined.
In the utility model, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " Gu It is fixed " etc. term should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integrally;Can be Mechanically connect or electrically connect;Can be joined directly together, can also be indirectly connected by intermediary, can be two The connection of element internal or the interaction relationship of two elements.For the ordinary skill in the art, can basis Concrete condition understands concrete meaning of the above-mentioned term in the utility model.
In the utility model, unless otherwise clearly defined and limited, fisrt feature is "above" or "below" second feature Can be that the first and second features directly contact, or the first and second features pass through intermediary mediate contact.Moreover, first is special Sign second feature " on ", " top " and " above " can be fisrt feature directly over second feature or oblique upper, or only Represent that fisrt feature level height is higher than second feature.Fisrt feature second feature " under ", " lower section " and " below " can be with Be fisrt feature immediately below second feature or obliquely downward, or be merely representative of fisrt feature level height and be less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description Point is contained at least one embodiment or example of the present utility model.In this manual, to the schematic table of above-mentioned term Identical embodiment or example need not be directed to by stating.Moreover, specific features, structure, material or the feature of description can be Combined in an appropriate manner in any one or more embodiments or example.In addition, in the case of not conflicting, this area Technical staff can be carried out the different embodiments or example and the feature of different embodiments or example described in this specification With reference to and combination.
Embodiment described above is only the preferred embodiment to absolutely prove the utility model and being lifted, the utility model Protection domain not limited to this.The equivalent substitute or change that those skilled in the art are made on the basis of the utility model Change, within the scope of protection of the utility model.The scope of protection of the utility model is defined by claims.

Claims (9)

1. the heat sink used during a kind of stem cell cryopreserving, it is characterised in that it includes:
Staving, it is sandwich design, including bottom is each the outer barrel and inner barr of sealing, and outer barrel is higher than interior bucket, outer barrel and interior On bucket annular top cover is formed along concordant and sealing therebetween, the open top of interior bucket, the thus shape between outer barrel and interior bucket Into the interlayer of sealing;
Sample adapter, it is a circular slab, and the outer of the circular slab matches with the open top of the interior bucket, on the circular slab One or more openings that can be matched with stem cell cryopreserving container are provided with, so that stem cell cryopreserving container is being inserted The inner chamber for entering the interior bucket after the opening forms relatively closed space.
2. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that the ring of the staving Refrigerant hand-hole and the stopple for sealing the refrigerant hand-hole are additionally provided with shape top cover.
3. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that the ring of the staving Refrigerant hand-hole is additionally provided with shape top cover and the stopple for sealing the refrigerant hand-hole, the stopple are connected by chain rope In annular top cover.
4. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that the sample adaptation Device is less than the depth of interior bucket configuring its height after sample is frozen.
5. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that the sample adaptation Opening on device circular slab is multiple circles, is matched for the top with centrifuge tube.
6. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that the sample adaptation Opening on device circular slab is one square, for being matched with freezing bag.
7. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that the sample adaptation A thermometer hole for being used to configure device for detecting temperature is also set up on device circular slab.
8. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that its staving and sample Adapter is to be made of clear material.
9. the heat sink used according to claim 1 during stem cell cryopreserving, it is characterised in that in the interior bucket Reinforcement is additionally provided with interlayer between bottom and outer barrel, for strengthening the structural strength between interior outer barrel and inner barr.
CN201720452653.2U 2017-04-27 2017-04-27 The heat sink used during stem cell cryopreserving Active CN206963793U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476955A (en) * 2019-09-16 2019-11-22 上海理工大学 A kind of programmed cell plant ice production apparatus

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110476955A (en) * 2019-09-16 2019-11-22 上海理工大学 A kind of programmed cell plant ice production apparatus
CN110476955B (en) * 2019-09-16 2021-07-20 上海理工大学 A cell-programmed ice planting device

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