CN219020054U - Tumor cell liquid preservation test tube - Google Patents
Tumor cell liquid preservation test tube Download PDFInfo
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- CN219020054U CN219020054U CN202223341085.9U CN202223341085U CN219020054U CN 219020054 U CN219020054 U CN 219020054U CN 202223341085 U CN202223341085 U CN 202223341085U CN 219020054 U CN219020054 U CN 219020054U
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Abstract
The utility model discloses a tumor cell liquid preservation test tube, which comprises a freezing tube and a sleeve, wherein the freezing tube is arranged in the sleeve, and the inner diameter of the sleeve is larger than the outer diameter of the freezing tube, so that an interlayer is formed between the outer wall of the freezing tube and the inner wall of the sleeve; the first sealing cover is in threaded connection with the top end of the freezing pipe; the sealing gasket is arranged at the top end of the interlayer; the second sealing cover is in threaded connection with the top end of the sleeve; wherein, the bottom middle part of freezer pipe with sheathed tube bottom middle part joint. Utilize the air sandwich structure between sleeve pipe outer wall and the freezing pipe inner wall to play the effect that slows down the air conditioning conduction in freezing process, and seal freezing pipe, sleeve pipe respectively through first sealed lid, second sealed lid, realize freezing pipe and sheathed tube detachable construction, the freezing pipe of being convenient for takes out independent processing, and the second grade is sealed helps improving the freezing effect to the cell sap.
Description
Technical Field
The utility model relates to the technical field of cell culture preservation, in particular to a tumor cell fluid preservation test tube.
Background
Cell cryopreservation is one of the main methods for cryopreserving tumor cells, and the cells are stored at low temperature in liquid nitrogen at-196 ℃ by using the cryopreservation technology, so that the cells can be temporarily separated from the growth state to store the cell characteristics, and the cells are resuscitated for experiments when needed. And a certain amount of cells are moderately preserved, so that the cells can be prevented from being lost due to pollution or other unexpected events of the cells being cultured, and the effect of preserving the cells is achieved. In addition, some cells may be purchased, gifted, exchanged, and transported in the form of cell cryopreservation. When the cells are frozen, a protective agent, i.e. glycerol or dimethyl sulfoxide (DMSO) with the final concentration of 5% -15%, is added into the culture medium, so that the freezing point of the solution can be reduced, and in addition, under the slow freezing condition, the water in the cells is penetrated out, and the formation of ice crystals is reduced, thereby avoiding the damage of the cells. The method of slow freezing and fast thawing can better ensure the survival of cells. The standard freezing speed is-1 to-2 ℃/min, and can be accelerated when the temperature is lower than-25 ℃ and can be directly put into liquid nitrogen (-196 ℃) after reaching-80 ℃.
However, the existing freezing tube has a single structure, which easily causes the cell to freeze too quickly, so that the water in the cell cannot be discharged, and ice crystals are generated to damage the cell. Chinese patent CN212393698U discloses a novel low temperature freezes deposits the pipe, be provided with the intermediate layer on the inner wall of freezing the pipe main part, the both sides of intermediate layer all are provided with through-hole mechanism, and through-hole mechanism is provided with two, the inside of freezing the pipe main part is provided with the cell and places the chamber, sealed lid is installed to the upper end of freezing the pipe main part. The interlayer is arranged to slow down the cold air conduction and delay the freezing speed of the cells. However, in this structure, the freezing tube main body and the interlayer are integrated, and the cell placement chamber inside the freezing tube main body cannot be used alone, and if rapid freezing or rapid thawing is required, the interlayer is also required to be used as an intermediary, thereby affecting the use effect.
Disclosure of Invention
The utility model aims to solve the problems in the technical background, and aims to provide a tumor cell liquid preservation test tube, wherein a sleeve is designed outside a freezing tube, an air sandwich structure between the outer wall of the sleeve and the inner wall of the freezing tube is utilized to play a role in slowing down cold air conduction in the freezing process, the freezing tube and the sleeve are respectively sealed through a first sealing cover and a second sealing cover, so that a separable structure of the freezing tube and the sleeve can be realized, the freezing tube is convenient to take out, thawing and other treatments are carried out independently, and the secondary sealing structure is beneficial to improving the freezing effect on cell liquid and has better sealing effect.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a tumor cell fluid preservation test tube comprising a cryotube, further comprising:
the refrigerating tube is arranged in the sleeve, and the inner diameter of the sleeve is larger than the outer diameter of the refrigerating tube, so that an interlayer is formed between the outer wall of the refrigerating tube and the inner wall of the sleeve;
the first sealing cover is in threaded connection with the top end of the freezing pipe;
the sealing gasket is arranged at the top end of the interlayer;
the second sealing cover is in threaded connection with the top end of the sleeve;
wherein, the bottom middle part of freezer pipe with sheathed tube bottom middle part joint.
Further, a boss is arranged at the bottom center of the freezing pipe, a groove is arranged at the inner center of the sleeve, and positioning and mounting of the freezing pipe are achieved through matching of the boss and the groove.
Still further, the outer surface of boss is provided with the external screw thread, the internal surface of recess is provided with the internal screw thread, through threaded connection with the freezing pipe is installed in the sleeve pipe.
In another technical scheme of the utility model, the novel automobile seat further comprises a placing seat, and a placing groove matched with the boss is formed in the middle of the placing seat.
Further, the inner diameter size of the placement groove is larger than the outer diameter size of the boss.
Further, when used after assembly, the top end of the sealing gasket abuts against the inside of the second sealing cover.
Further, an arc-shaped holding groove which is concave is formed in the middle of the outer wall of the sleeve, and the holding groove and the sleeve are of an integrated structure.
Compared with the prior art, the utility model has the following advantages:
1. according to the utility model, the sleeve is arranged outside the freezing pipe, the air sandwich structure between the outer wall of the sleeve and the inner wall of the freezing pipe plays a role in slowing down cold air conduction in the freezing process, the freezing speed of cells is delayed, the freezing pipe and the sleeve are respectively sealed through the first sealing cover and the second sealing cover, the detachable structure of the freezing pipe and the sleeve can be realized, the freezing pipe is convenient to take out, the independent thawing treatment and the like are carried out, and the secondary sealing structure is beneficial to improving the freezing effect on cell liquid and has better sealing effect.
2. According to the utility model, the refrigerating tube can be stably placed in the sleeve through the matching of the boss and the groove, the floating problem of the refrigerating tube can not occur when the refrigerating tube is frozen in liquid nitrogen, the refrigerating effect is more uniform, and after thawing, the refrigerating tube is stably placed in the placing seat by utilizing the boss, so that the refrigerating tube is not easy to topple and convenient to use.
Drawings
FIG. 1 is a schematic view showing a three-dimensional exploded structure of a preservation test tube of the present utility model;
FIG. 2 is a cross-sectional view of the preservation tube of FIG. 1;
FIG. 3 is a schematic cross-sectional view of the present utility model when used with a test tube;
FIG. 4 is a schematic diagram of a storage tube according to another embodiment of the present utility model;
fig. 5 is a schematic structural diagram of a cooling tube and a placement seat according to an embodiment of the utility model.
In the figure: 1. a freezing tube; 2. a sleeve; 3. a first sealing cover; 4. a sealing gasket; 5. a second sealing cover; 6. an interlayer; 7. a boss; 8. a groove; 9. an external thread; 10. an internal thread; 11. a placement seat; 12. a placing groove, 13 and a holding groove.
Detailed Description
The following describes specific embodiments of the present utility model in detail with reference to the drawings. It should be understood that the detailed description and specific examples, while indicating and illustrating the utility model, are not intended to limit the utility model.
As shown in fig. 1-5, a tumor cell liquid preservation test tube comprises a freezing tube 1, and further comprises:
a sleeve 2, wherein the freezing pipe 1 is arranged inside the sleeve 2, and the inner diameter of the sleeve 2 is larger than the outer diameter of the freezing pipe 1, so that an interlayer 6 is formed between the outer wall of the freezing pipe 1 and the inner wall of the sleeve 2;
the first sealing cover 3 is in threaded connection with the top end of the freezing pipe 1;
a sealing gasket 4, the sealing gasket 4 being arranged at the top end of the interlayer 6;
the second sealing cover 5 is in threaded connection with the top end of the sleeve 2;
wherein, the bottom middle part of the freezing pipe 1 is clamped with the bottom middle part of the sleeve 2.
According to the utility model, the sleeve 2 is arranged outside the freezing pipe 1, the air interlayer 6 structure between the outer wall of the sleeve 2 and the inner wall of the freezing pipe 1 is used for slowing down cold air conduction in the freezing process, the freezing speed of cells is delayed, the freezing pipe 1 and the sleeve 2 are respectively sealed through the first sealing cover 3 and the second sealing cover 5, the separable structure of the freezing pipe 1 and the sleeve 2 can be realized, the freezing pipe 1 is conveniently taken out, thawing and other treatments are independently carried out, and the secondary sealing structure is beneficial to improving the freezing effect on cell liquid and has better sealing effect.
As shown in fig. 2 and 3, in the present embodiment:
the bottom center of the freezing pipe 1 is provided with a boss 7, the inner center of the sleeve 2 is provided with a groove 8, and the positioning and the installation of the freezing pipe 1 are realized through the matching of the boss 7 and the groove 8.
In this embodiment, the cooperation of boss 7 and recess 8 is utilized to make freezing pipe 1 can firmly place in sleeve pipe 2, and freezing pipe 1 can not take place the skew for sleeve pipe 2, when freezing in the liquid nitrogen, makes the test tube submergence in the liquid nitrogen with the gravity of sleeve pipe 2, can not take place the problem that freezing pipe 1 floats, and the freezing effect is more even.
As shown in fig. 4, in another embodiment:
the outer surface of the boss 7 is provided with an external thread 9, the inner surface of the groove 8 is provided with an internal thread 10, and the freezing pipe 1 is installed in the sleeve 2 through threaded connection.
The external threads 9 on the outer surface of the boss 7 are matched with the internal threads 10 in the groove 8, so that the connection stability of the freezing pipe 1 and the sleeve 2 is further improved.
As shown in fig. 5, in another technical solution of the present utility model, the device further includes a placement seat 11, and a placement groove 12 matched with the boss 7 is disposed in the middle of the placement seat 11.
Further, the inner diameter dimension of the placement groove 12 is larger than the outer diameter dimension of the boss 7.
After thawing, the boss 7 is matched with the placing groove 12, so that the freezing pipe 1 is stably placed in the placing seat 11, and the freezing pipe is not easy to topple over and convenient to use.
As shown in fig. 3, when assembled, the tip of the sealing gasket 4 abuts against the inside of the second sealing cover 5.
The sealing gasket 4 is used to improve the tightness of the second sealing cover 5 so as to prevent liquid nitrogen or other media from entering the interlayer 6.
As shown in fig. 1, in the present utility model:
the middle part of the outer wall of the sleeve 2 is provided with an inward concave arc-shaped holding groove 13, and the holding groove 13 and the sleeve 2 are of an integrated structure.
The arc-shaped holding groove 13 is used for facilitating the clamping of the sleeve 2 by using a tool, so that the assembled freezing pipe 1 and the sleeve 2 are placed in liquid nitrogen or taken out from the liquid nitrogen, and the liquid nitrogen is prevented from damaging a human body.
The foregoing has shown and described the basic principles, principal features and advantages of the utility model. It will be understood by those skilled in the art that the present utility model is not limited to the embodiments described above, and that the above embodiments and descriptions are merely illustrative of the principles of the present utility model, and various changes and modifications may be made therein without departing from the spirit and scope of the utility model, which is defined by the appended claims. The scope of the utility model is defined by the appended claims and equivalents thereof.
Claims (7)
1. A tumor cell sap preservation test tube, comprising a freezing tube, characterized by further comprising:
the refrigerating tube is arranged in the sleeve, and the inner diameter of the sleeve is larger than the outer diameter of the refrigerating tube, so that an interlayer is formed between the outer wall of the refrigerating tube and the inner wall of the sleeve;
the first sealing cover is in threaded connection with the top end of the freezing pipe;
the sealing gasket is arranged at the top end of the interlayer;
the second sealing cover is in threaded connection with the top end of the sleeve;
wherein, the bottom middle part of freezer pipe with sheathed tube bottom middle part joint.
2. The tumor cell sap preservation test tube according to claim 1, wherein a boss is arranged at the bottom center of the freezing tube, a groove is arranged at the inner center of the sleeve, and positioning and mounting of the freezing tube are achieved through matching of the boss and the groove.
3. The test tube of claim 2, wherein the outer surface of the boss is provided with external threads, the inner surface of the recess is provided with internal threads, and the freezing tube is mounted in the sleeve by threaded connection.
4. The tumor cell sap preservation test tube according to claim 2, further comprising a placement seat, wherein a placement groove matched with the boss is provided in the middle of the placement seat.
5. The test tube of claim 4, wherein the inner diameter of the groove is greater than the outer diameter of the boss.
6. A test tube for preserving tumor cell fluid according to claim 1, wherein the tip of said sealing gasket abuts against the inside of said second sealing cap when assembled for use.
7. The test tube for preserving tumor cell sap according to claim 1, wherein the middle part of the outer wall of the sleeve is provided with an inward concave arc-shaped holding groove, and the holding groove and the sleeve are of an integral structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223341085.9U CN219020054U (en) | 2022-12-13 | 2022-12-13 | Tumor cell liquid preservation test tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202223341085.9U CN219020054U (en) | 2022-12-13 | 2022-12-13 | Tumor cell liquid preservation test tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN219020054U true CN219020054U (en) | 2023-05-16 |
Family
ID=86277234
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202223341085.9U Active CN219020054U (en) | 2022-12-13 | 2022-12-13 | Tumor cell liquid preservation test tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN219020054U (en) |
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2022
- 2022-12-13 CN CN202223341085.9U patent/CN219020054U/en active Active
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