CN211458672U - Cryopreserved cell transfer device - Google Patents
Cryopreserved cell transfer device Download PDFInfo
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- CN211458672U CN211458672U CN201922362290.5U CN201922362290U CN211458672U CN 211458672 U CN211458672 U CN 211458672U CN 201922362290 U CN201922362290 U CN 201922362290U CN 211458672 U CN211458672 U CN 211458672U
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- refrigerant
- cell transfer
- box
- transfer device
- cryopreserved cell
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Abstract
The utility model discloses a cryopreserved cell transfer device relates to cell biotechnology field, including the fridge, the refrigerant hold up tank, refrigerant adsorption equipment with cryopreserved pipe lays the device, the fridge includes box and case lid, the box rotates with the case lid to be connected, the internal fixation of box is provided with the refrigerant hold up tank, be equipped with refrigerant adsorption equipment in the refrigerant hold up tank, cryopreserved pipe lays the device and includes flat board and a plurality of fixture, be equipped with the refrigerant filling opening on the flat board, it has a plurality of card holes to open on the flat board, fixture is the same with the quantity in card hole, the one end of each fixture respectively with each card hole fixed connection, the other end stretches into in the refrigerant adsorption equipment. The utility model discloses in, in being in the low temperature environment during the cell is transported, the transportation of the cell short time of being convenient for makes the cell be difficult for dying, sets up the fridge, and convenient cleanness can prevent to pollute, sets up and freezes to deposit the pipe and lay the device and can prevent to obscure, sets up the security that the refrigerant filling opening can improve the experimenter operation.
Description
Technical Field
The utility model relates to a cell biotechnology field especially relates to a cryopreserved cell transfer device.
Background
The cell freezing storage is a technology for placing cells in a low-temperature environment to reduce cell metabolism so as to be stored for a long time, during passage and daily maintenance of cell culture, once the cells leave a living body and start primary culture, various biological characteristics of the cells gradually change and continuously have new changes along with the increase of passage times and the change of in vitro environmental conditions, and the cells can be stored for a long time in a refrigerator at the temperature of minus 80 ℃; the cells were stored in liquid nitrogen at a temperature of-196 ℃ for a theoretically unlimited storage time.
According to the management requirement of GMP to a laboratory, a cell bank and the laboratory are managed in a partition mode, even the cell bank and the laboratory are not on the same experimental floor or the same experimental building, so that a lot of difficulties are brought to cell transfer, the death risk of cells can be increased in a long-time transfer process, the cells are transferred in a foam box or a program cooling box filled with liquid nitrogen in the conventional transfer method, however, the foam box is not easy to wipe, the environment pollution can be caused to the clean environment of the laboratory, the requirement is high, the repeated utilization rate is low, the environment is not protected, the liquid nitrogen is easy to volatilize in the foam box and cannot be transported for a long time, confusion can be easily caused when different types of cells are transferred simultaneously, a freezing tube is completely positioned in the liquid nitrogen, and testers are easy to hurt when taking the freezing tube; the temperature of the program cooling box is not controllable and can only be automatically controlled by the program cooling box, and once the cover opening temperature is rapidly increased, the application range is small and the practicability is poor.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a cryopreserved cell transfer device to solve the problem that above-mentioned prior art exists, shorten the transit time of cell, reduce the cell and obscure and dead risk in the transfer process, the experimenter security is high.
In order to achieve the above object, the utility model provides a following scheme:
the utility model provides a cryopreserved cell transfer device, deposit the device including fridge, refrigerant hold up tank, refrigerant adsorption equipment and cryopreserved pipe, the fridge includes box and case lid, the box with the case lid rotates to be connected, the box internal fixation is provided with the refrigerant hold up tank, be equipped with in the refrigerant hold up tank refrigerant adsorption equipment, cryopreserved pipe is laid the device and is included dull and stereotyped and a plurality of fixture, be equipped with the refrigerant filling opening on the dull and stereotyped, it has a plurality of card holes to open on the dull and stereotyped, fixture with the quantity in card hole is the same, each the one end of fixture respectively with each card hole fixed connection, each the other end of fixture stretches into in the refrigerant adsorption equipment, fixture are used for supporting cryopreserved pipe and prevent cryopreserved pipe and refrigerant direct contact.
Preferably, the refrigerant adsorption device is superfine glass fiber cotton.
Preferably, the lower end surface of the flat plate is fixedly connected with the upper end surface of the refrigerant storage tank.
Preferably, a sealing gasket is fixedly arranged on the upper end surface of the refrigerant storage groove, and the sealing gasket is positioned between the flat plate and the refrigerant storage groove.
Preferably, a cock is arranged on the refrigerant injection port, and a vent hole is formed in the cock.
Preferably, the refrigerating box is provided with a lock, and the lock is used for limiting the relative movement of the box cover and the box body.
Preferably, the box body is provided with a sealing strip, and the sealing strip is positioned between the box body and the box cover.
Preferably, the plate is integrally formed with each of the fixtures.
Preferably, the freezing storage pipe placing device and the refrigerant storage tank are both made of stainless steel.
The utility model discloses for prior art gain following technological effect:
the utility model provides a frozen cell transfer device, which is provided with a refrigerating box, has small volume and easy cleaning, can prevent the laboratory from being polluted by external source, one side edge of a box cover is connected with one side edge of a box body, the box cover and the box body can rotate relatively around a horizontal axis, so that the switch of the refrigerating box is simple and convenient, a refrigerant storage tank is fixedly arranged in the box body, a refrigerant adsorption device is arranged in the refrigerant storage tank, a frozen tube placing device comprises a flat plate and a plurality of clamps, a refrigerant injection opening is arranged on the flat plate, after the device is assembled and the frozen tube is placed, a funnel is placed on the refrigerant injection opening, the refrigerant is filled in the refrigerant storage tank, the refrigerant adsorption device adsorbs the refrigerant in the refrigerant storage tank, the cells in the frozen tube are transferred in a low-temperature environment, the vitality of the cells in the transferring process is ensured, in addition, an experimenter, the security is high, it has a plurality of card holes to open on the flat board, fixture is the same with the quantity in card hole, the one end of each fixture respectively with each card hole fixed connection, when different kinds of cell was transported simultaneously, the freezing of holding the cell is deposited the pipe and can be put by the subregion, prevent to obscure, the other end of each fixture stretches into in the refrigerant absorption device, fixture is used for supporting the freezing and deposits the pipe and prevents to freeze and deposit pipe and refrigerant direct contact, slow freezing, make the intracellular moisture ooze outside the cell, prevent to lead to the intracellular formation ice crystal because of freezing speed is too fast, cause the damage to the cell, still can prevent to freeze and deposit the pipe because of the refrigeration effect of refrigerant embrittlement.
Furthermore, the refrigerant adsorption device is made of superfine glass fiber cotton, fine air holes are formed in the superfine glass fiber cotton, so that liquid nitrogen can be adsorbed, and the superfine glass fiber cotton is good in heat insulation performance and not prone to embrittlement.
Further, dull and stereotyped lower terminal surface and the up end fixed connection of refrigerant hold up tank for at the transfer process, the cryopreserved pipe is laid and is not taken place relative motion between device and the refrigerant hold up tank, prevents to cryopreserved pipe and overturns, guarantees the security of transfer process.
Furthermore, a sealing gasket is fixedly arranged on the upper end face of the refrigerant storage groove, is positioned between the flat plate and the refrigerant storage groove and is used for sealing the connecting part of the flat plate and the refrigerant storage groove, and the heat preservation effect is prevented from being reduced due to the fact that liquid nitrogen escapes from the connecting part of the flat plate and the refrigerant storage groove.
Furthermore, a cock is arranged on the refrigerant injection port, a vent hole is formed in the cock, the cock plugs the refrigerant injection port after the refrigerant injection is completed, and the liquid nitrogen volatilizes after absorbing heat and can escape from the vent hole, so that the phenomenon that the pressure in the sealing cavity is overlarge due to the sealing between the freezing storage pipe placing device and the refrigerant storage tank is prevented.
Furthermore, the refrigerating box is provided with a lock which is used for limiting the relative motion of the box cover and the box body, so that the refrigerating box is in a closed state in the transfer process, the box cover is not easy to accidentally open, the freezing tube is protected, and the safety of the freezing tube in the refrigerating box is ensured.
Furthermore, a sealing strip is arranged on the box body and is positioned between the box body and the box cover, and the sealing strip is used for sealing the refrigerating box, so that the refrigerant is prevented from overflowing and the safety of experimenters is threatened.
Furthermore, the flat plate and the clamping devices are integrally formed, so that the processing is convenient, and the structural strength of the frozen pipe placing device can be ensured.
Furthermore, the freezing storage pipe placing device and the refrigerant storage tank are made of stainless steel materials, so that the inside of the device is convenient to clean, liquid leakage can be prevented, and the freezing storage pipe placing device and the refrigerant storage tank are not easy to embrittle due to freezing.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
Fig. 1 is a schematic structural diagram of a cryopreserved cell transfer device provided by the present invention;
fig. 2 is a schematic structural view of a cryopreservation tube placing device of the cryopreservation cell transfer device provided by the present invention;
fig. 3 is the structural schematic diagram of the refrigerant storage tank of the cryopreservation cell transfer device provided by the present invention.
In the figure: 1-a refrigerant storage tank; 2-a box body; 3-box cover; 4-plate; 5-a fixture; 6-clamping hole; 7-refrigerant injection port; 8-a lockset.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The utility model aims at providing a cryopreserved cell transfer device to solve the problem that prior art exists, shorten the transit time of cell, reduce the cell and obscure and dead risk in the transfer process, the experimenter security is high.
In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention is described in detail with reference to the accompanying drawings and the detailed description.
The utility model provides a frozen cell transfer device, as shown in figures 1-3, in this embodiment, the frozen cell transfer device comprises a refrigeration box, a refrigerant storage tank 1, a refrigerant adsorption device and a frozen tube placing device, the refrigerant is exemplified by liquid nitrogen, but not limited to liquid nitrogen, the refrigeration effect of the liquid nitrogen is good, nontoxic, inactive and convenient to prepare, the refrigeration box is provided, the volume is small, the cleaning is easy, and the laboratory can be prevented from being polluted by external source, the refrigeration box comprises a box body 2 and a box cover 3, one side edge of the box body 2 is connected with one side edge of the box cover 3, the box cover 3 and the box body 2 can rotate relatively around the horizontal axis, so that the refrigeration box is simple and convenient to open and close, the refrigerant storage tank 1 is fixedly arranged in the box body 2, the refrigerant storage tank 1 is internally provided with the refrigerant adsorption device, the frozen tube placing device comprises a flat plate 4 and a plurality of, the flat plate is provided with a refrigerant injection hole 7, after the device is assembled and placed with a freezing tube, a funnel is placed on the refrigerant injection hole 7, refrigerant is filled into the refrigerant storage tank 1, the refrigerant adsorption device adsorbs the refrigerant in the refrigerant storage tank 1, cells in the freezing tube are transported in a low-temperature environment, the activity of the cells in the transportation process is guaranteed, in addition, an experimenter does not contact with the refrigerant, the safety is high, the flat plate is provided with a plurality of clamping holes 6, the number of the clamping devices 5 is the same as that of the clamping holes 6, one end of each clamping device 5 is fixedly connected with each clamping hole 6, when different types of cells are transported simultaneously, the freezing tube for containing the cells can be placed in different areas to prevent confusion, the other end of each clamping device 5 extends into the refrigerant adsorption device, the clamping devices 5 are used for supporting the freezing tube and preventing the freezing tube from directly contacting with the refrigerant, and are slowly frozen, the water in the cells seeps out of the cells, the ice crystals formed in the cells due to the over-high freezing speed are prevented from damaging the cells, and the freezing storage pipe is prevented from being embrittled due to the freezing action of the refrigerant.
Furthermore, the refrigerant adsorption device is made of superfine glass fiber cotton, fine air holes are formed in the superfine glass fiber cotton, so that liquid nitrogen can be adsorbed, and the superfine glass fiber cotton is good in heat insulation performance and not prone to embrittlement.
Further, dull and stereotyped 4 lower terminal surface and the up end fixed connection of refrigerant hold up tank 1 for at the transportation, freezing and depositing and do not take place relative motion between pipe laying device and the refrigerant hold up tank 1, prevent to freeze and deposit the pipe upset, guarantee the security of transportation.
Furthermore, a sealing gasket is fixedly arranged on the upper end face of the refrigerant storage tank 1, the sealing gasket is positioned between the flat plate 4 and the refrigerant storage tank 1 and is used for sealing the connecting part of the flat plate 4 and the refrigerant storage tank 1, and the heat preservation effect is prevented from being reduced due to the fact that liquid nitrogen escapes from the connecting part of the flat plate 4 and the refrigerant storage tank 1.
Furthermore, a cock is arranged on the refrigerant injection port 7, a vent hole is formed in the cock, the cock plugs the refrigerant injection port after the refrigerant injection is completed, and the liquid nitrogen volatilizes after absorbing heat and can escape from the vent hole, so that the phenomenon that the pressure in the sealing cavity is overlarge due to the sealing between the freezing storage pipe placing device and the refrigerant storage tank 1 is avoided.
Further, be equipped with tool to lock 8 on the fridge, tool to lock 8 is used for restricting the relative motion of case lid 3 and box 2 for the fridge is in the closed condition among the transportation, and case lid 3 is difficult for unexpected the opening, and the protection freezes deposits the pipe, guarantees to freeze the security of depositing the pipe in the fridge.
Further, be equipped with the sealing strip on the box 2, the sealing strip is located between box 2 and the case lid 3 and the sealing strip is used for sealed fridge to prevent that the refrigerant is excessive, threatens laboratory staff's safety.
Furthermore, the flat plate 4 and the clamps 5 are integrally formed, so that the processing is convenient, and the structural strength of the frozen pipe placing device can be ensured.
Further, the freezing storage pipe placing device and the refrigerant storage tank 1 are made of stainless steel materials, so that the inside of the device is convenient to clean, liquid leakage can be prevented, and the freezing storage pipe placing device and the refrigerant storage tank 1 are not prone to embrittlement due to freezing.
The utility model discloses a concrete example is applied to explain the principle and the implementation mode of the utility model, and the explanation of the above example is only used to help understand the method and the core idea of the utility model; meanwhile, for the general technical personnel in the field, according to the idea of the present invention, there are changes in the concrete implementation and the application scope. In summary, the content of the present specification should not be construed as a limitation of the present invention.
Claims (9)
1. The utility model provides a cryopreserved cell transfer device which characterized in that: the freezing storage device comprises a freezing storage box, a refrigerant storage tank, a refrigerant adsorption device and a freezing storage pipe placing device, wherein the freezing storage box comprises a box body and a box cover, the box body is rotatably connected with the box cover, the refrigerant storage tank is fixedly arranged in the box body, the refrigerant adsorption device is arranged in the refrigerant storage tank, the freezing storage pipe placing device comprises a flat plate and a plurality of clamping devices, a refrigerant filling opening is formed in the flat plate, a plurality of clamping holes are formed in the flat plate, the number of the clamping devices is the same as that of the clamping holes, one end of each clamping device is fixedly connected with each clamping hole, the other end of each clamping device extends into the refrigerant adsorption device, and the clamping devices are used for supporting the freezing storage pipe and preventing the freezing storage pipe from directly contacting with the refrigerant.
2. The cryopreserved cell transfer device of claim 1, wherein: the refrigerant adsorption device is made of superfine glass fiber cotton.
3. The cryopreserved cell transfer device of claim 1, wherein: the lower end face of the flat plate is fixedly connected with the upper end face of the refrigerant storage tank.
4. The cryopreserved cell transfer device of claim 3, wherein: and a sealing gasket is fixedly arranged on the upper end surface of the refrigerant storage groove and is positioned between the flat plate and the refrigerant storage groove.
5. The cryopreserved cell transfer device of claim 1, wherein: the refrigerant injection port is provided with a cock, and the cock is provided with a vent hole.
6. The cryopreserved cell transfer device of claim 1, wherein: the refrigerator is characterized in that the refrigerator is provided with a lock, and the lock is used for limiting the relative movement of the refrigerator cover and the refrigerator body.
7. The cryopreserved cell transfer device of claim 1, wherein: the box body is provided with a sealing strip, and the sealing strip is positioned between the box body and the box cover.
8. The cryopreserved cell transfer device of claim 1, wherein: the flat plate and each fixture are integrally formed.
9. The cryopreserved cell transfer device of claim 1, wherein: the freezing storage pipe placing device and the refrigerant storage tank are both made of stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922362290.5U CN211458672U (en) | 2019-12-25 | 2019-12-25 | Cryopreserved cell transfer device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922362290.5U CN211458672U (en) | 2019-12-25 | 2019-12-25 | Cryopreserved cell transfer device |
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| Publication Number | Publication Date |
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| CN211458672U true CN211458672U (en) | 2020-09-11 |
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| CN201922362290.5U Active CN211458672U (en) | 2019-12-25 | 2019-12-25 | Cryopreserved cell transfer device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117685717A (en) * | 2024-01-31 | 2024-03-12 | 福建北记食品有限公司 | Refrigerating device for preparing cake ingredients |
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2019
- 2019-12-25 CN CN201922362290.5U patent/CN211458672U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117685717A (en) * | 2024-01-31 | 2024-03-12 | 福建北记食品有限公司 | Refrigerating device for preparing cake ingredients |
| CN117685717B (en) * | 2024-01-31 | 2024-04-30 | 福建北记食品有限公司 | Refrigerating device for preparing cake ingredients |
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