CN213639439U

CN213639439U - Cell cryopreservation kit

Info

Publication number: CN213639439U
Application number: CN202022725552.2U
Authority: CN
Inventors: 梅志刚; 张佳; 曾伟
Original assignee: Guangzhou Sihe Biotechnology Co ltd
Current assignee: Guangzhou Sihe Biotechnology Co ltd
Priority date: 2020-11-23
Filing date: 2020-11-23
Publication date: 2021-07-09
Anticipated expiration: 2030-11-23

Abstract

The utility model discloses a cell cryopreserving kit relates to the cell cryopreserving field, and the process that needs to current cell cryopreserving is more loaded down with trivial details, and needs to be transported and deposit, and current kit can only deposit simultaneously, can't carry out independently refrigerated problem, presents following scheme now, and it includes the box, box internally mounted has vertical division board, the inside test tube standing groove that is provided with of box, the box top is provided with the case lid, be provided with the connecting piece between case lid and the box, the connecting piece carries out threaded connection through fixed screw and box and case lid, the inside horizontal division board that is provided with of box. The utility model discloses novel structure, and can make the cell once only accomplish freezing work in the reagent box, need not the secondary and transport, save time, promoted the leakproofness of device simultaneously, and can obtain real-time supervision to the inside temperature of device, make things convenient for the staff to observe.

Description

Cell cryopreservation kit

Technical Field

The utility model relates to a cell cryopreserving field especially relates to a cell cryopreserving kit.

Background

When the experiment is performed again, a cell experiment usually needs to be continued for a period of time, and a cell cryopreservation process is needed, wherein the cell cryopreservation is one of the main methods for cell preservation. The cell is placed in liquid nitrogen at the temperature of-196 ℃ for low-temperature preservation by utilizing a cryopreservation technology, so that the cell can be temporarily separated from a growth state and the cell characteristic can be preserved, the cell is recovered for experiment when needed, the cell is usually frozen to the temperature below-80 ℃ when the cell is cryopreserved, then the test tube is transferred into the liquid nitrogen solution, the operation is complex, and the cell cryopreservation kit is provided for solving the problems.

Disclosure of Invention

The utility model provides a cell cryopreserving kit has solved the process that current cell cryopreserved needs and is more loaded down with trivial details, and need transport and deposit, and current kit can only deposit simultaneously, can't carry out independently refrigerated problem.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

a cell cryopreservation kit comprises a box body, wherein a vertical partition plate is arranged inside the box body, a test tube placing groove is arranged inside the box body, a box cover is arranged at the top of the box body, a connecting piece is arranged between the box cover and the box body and is in threaded connection with the box body and the box cover through a fixing screw, a transverse partition plate is arranged inside the box body, fillers are arranged between the test tube placing groove and the transverse partition plate as well as between the test tube placing groove and the vertical partition plate, a handle is arranged on the upper surface of the box cover, a temperature sensor is arranged inside the box cover, a bottom plate is arranged inside the box body and is arranged below the test tube placing groove, a liquid nitrogen bottle is arranged at the bottom of the box body, a valve is arranged at the top of the liquid nitrogen bottle, a tray is arranged inside the, the bottom of telescopic link is installed in the inside bottom of test tube standing groove, the telescopic link top is installed at the tray lower surface, the refrigerator is installed to the tray below.

Preferably, the box is divided into four parts by the transverse partition plates and the vertical partition plates, the number of the test tube placing grooves is four, the four test tube placing grooves are uniformly installed in the four parts of the box, and fillers are arranged among the test tube placing grooves, the transverse partition plates and the vertical partition plates.

Preferably, a temperature sensor is mounted at the position of the box cover corresponding to each test tube placing groove, the model of the temperature sensor is TELESKY-DS18B20, and the bottom of each test tube placing groove is provided with a liquid nitrogen bottle.

Preferably, the tray is provided with four, and four the tray is evenly installed in every test tube standing groove, every two telescopic links are all installed to the tray bottom, and every a refrigerator is all installed to the tray bottom.

Preferably, the bottom of the box cover and the top of the box body are both provided with sealing gaskets.

Preferably, the connecting pieces are arranged between the box cover and the box body, and rotating shafts are arranged in the two connecting pieces.

The utility model has the advantages that:

1. through the integrated design of the kit, the cells can finish the freezing work in the kit at one time without secondary transportation, thereby saving time and improving efficiency.

2. The sealing gaskets arranged at the bottom of the box cover and the top of the box body can improve the sealing performance of the device, so that the device can be frozen for a long time.

3. Through temperature sensor, can make the inside temperature of device obtain real-time supervision, make things convenient for the staff to observe.

To sum up, the device not only can make the cell once only accomplish the work of freezing in the reagent box, need not the secondary and transports, saves time, has promoted the leakproofness of device simultaneously, and can obtain real-time supervision to the inside temperature of device, makes things convenient for the staff to observe, and it is more loaded down with trivial details to have solved the process that current cell freezes to deposit needs, and needs to transport and deposit, and current kit can only deposit simultaneously, can't carry out the problem of independently freezing.

Drawings

Fig. 1 is a schematic structural diagram of the present invention.

Fig. 2 is a front sectional view of the present invention.

Fig. 3 is an enlarged view of a portion a in fig. 2 according to the present invention.

Reference numbers in the figures: 1. a box body; 2. a vertical partition plate; 3. a test tube placing groove; 4. a connecting member; 5. fixing screws; 6. a gasket; 7. a filler; 8. a transverse divider plate; 9. a box cover; 10. a temperature sensor; 11. a handle; 12. a protective solution; 13. a liquid nitrogen bottle; 14. a base plate; 15. a valve; 16. a tray; 17. a telescopic rod; 18. a refrigerator.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-3, a cell cryopreservation kit comprises a box body 1, wherein a vertical partition plate 2 is installed inside the box body 1, a test tube placing groove 3 is arranged inside the box body 1, a box cover 9 is arranged on the top of the box body 1, a connecting piece 4 is arranged between the box cover 9 and the box body 1, the connecting piece 4 is in threaded connection with the box body 1 and the box cover 9 through a fixing screw 5, a transverse partition plate 8 is arranged inside the box body 1, a filler 7 is arranged between the test tube placing groove 3 and the transverse partition plate 8 as well as the vertical partition plate 2, a handle 11 is installed on the upper surface of the box cover 9, a temperature sensor 10 is installed inside the box cover 9, a bottom plate 14 is installed inside the box body 1, the bottom plate 14 is installed below the test tube placing groove 3, a liquid nitrogen bottle 13 is installed at the bottom, a tray 16 is arranged inside the test tube placing groove 3, a protective solution 12 is arranged inside the test tube placing groove 3, an expansion link 17 is arranged at the bottom of the tray 16, the bottom of the expansion link 17 is arranged at the bottom inside the test tube placing groove 3, the top of the expansion link 17 is arranged at the lower surface of the tray 16, a refrigerator 18 is arranged below the tray 16, the inside of the box body 1 is divided into four parts by the transverse partition plates 8 and the vertical partition plates 2, the number of the test tube placing grooves 3 is four, the four test tube placing grooves 3 are uniformly arranged in the four parts of the box body 1, a filler 7 is arranged between each test tube placing groove 3 and the transverse partition plate 8 as well as between each test tube placing groove 3 and the vertical partition plate 2, a temperature sensor 10 is arranged at the position of a box cover 9 corresponding to each test tube placing groove 3, the model of the temperature sensor 10 is TELESKY-DS18B20, and the bottom, tray 16 is provided with four, and four tray 16 is evenly installed in every test tube standing groove 3, every two telescopic links 17, and every are all installed to tray 16 bottom a refrigerator 18 is all installed to tray 16 bottom, case lid 9 bottom and 1 top of box all are provided with sealed 6, connecting piece 4 is provided with two, and two between case lid 9 and box 1 connecting piece 4 is inside all to be provided with the pivot.

The working principle is as follows: when the device is used, firstly, the box cover 9 is opened through the handle 11, then the test tube placing groove 3 in the box body 1 is filled with the protective liquid 12, then the cell test tube is placed on the surface of the tray 16 in the test tube placing groove 3, then the box cover 9 is closed through the handle 11 and the connecting piece 4 and is sealed through the sealing gasket 6, then the refrigerator 18 is opened, the temperature in the box is detected through the temperature sensor 10, after the temperature reaches below minus 80 ℃, the liquid nitrogen valve 15 is opened, liquid nitrogen in the liquid nitrogen bottle 13 is filled in the box, the temperature in the box is reduced to below minus 196 ℃, when the box is unfrozen, the tray 16 is lifted up through the telescopic rod 17, the worker protects the box and prevents frostbite.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The cell cryopreservation kit comprises a box body (1) and is characterized in that a vertical partition plate (2) is arranged inside the box body (1), a test tube placing groove (3) is arranged inside the box body (1), a box cover (9) is arranged at the top of the box body (1), a connecting piece (4) is arranged between the box cover (9) and the box body (1), the connecting piece (4) is in threaded connection with the box body (1) and the box cover (9) through a fixing screw (5), a transverse partition plate (8) is arranged inside the box body (1), a filler (7) is arranged between the test tube placing groove (3) and the transverse partition plate (8) as well as between the test tube placing groove and the vertical partition plate (2), a handle (11) is arranged on the upper surface of the box cover (9), a temperature sensor (10) is arranged inside the box cover (9), and a bottom plate (14), the utility model discloses a test tube storage device, including bottom plate (14), liquid nitrogen bottle (13) are installed to box (1) bottom, liquid nitrogen bottle (13) top is provided with valve (15), test tube storage groove (3) internally mounted has tray (16), test tube storage groove (3) inside is provided with protection liquid (12), tray (16) bottom is provided with telescopic link (17), the bottom of telescopic link (17) is installed in test tube storage groove (3) inside bottom, install at tray (16) lower surface telescopic link (17) top, refrigerator (18) are installed to tray (16) below.

2. The cell cryopreservation kit according to claim 1, wherein the inside of the box body (1) is divided into four parts by the transverse partition plate (8) and the vertical partition plate (2), the number of the test tube placing grooves (3) is four, the four test tube placing grooves (3) are uniformly arranged in the four parts of the box body (1), and a filler (7) is arranged between each test tube placing groove (3) and the transverse partition plate (8) and the vertical partition plate (2).

3. The cell cryopreservation kit according to claim 2, wherein a temperature sensor (10) is mounted at the position of the box cover (9) corresponding to each test tube placing groove (3), the model of the temperature sensor (10) is TELESKY-DS18B20, and the bottom of each test tube placing groove (3) is provided with a liquid nitrogen bottle (13).

4. The cell cryopreservation kit according to claim 1, wherein four trays (16) are provided, four trays (16) are uniformly arranged in each test tube placing groove (3), two telescopic rods (17) are arranged at the bottom of each tray (16), and a refrigerating machine (18) is arranged at the bottom of each tray (16).

5. The cell cryopreservation kit according to claim 1, wherein the bottom of the box cover (9) and the top of the box body (1) are provided with sealing gaskets (6).

6. The cell cryopreservation kit according to claim 1, wherein two connecting pieces (4) are arranged between the box cover (9) and the box body (1), and rotating shafts are arranged inside the two connecting pieces (4).