CN215708639U - Split mounting type wet-turning cooling box for immunoblotting test - Google Patents
Split mounting type wet-turning cooling box for immunoblotting test Download PDFInfo
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- CN215708639U CN215708639U CN202122004754.2U CN202122004754U CN215708639U CN 215708639 U CN215708639 U CN 215708639U CN 202122004754 U CN202122004754 U CN 202122004754U CN 215708639 U CN215708639 U CN 215708639U
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- bottom plate
- cooling box
- plate
- side plates
- freezing
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- 238000001816 cooling Methods 0.000 title claims abstract description 62
- 238000003119 immunoblot Methods 0.000 title claims abstract description 12
- 238000012360 testing method Methods 0.000 title claims abstract description 12
- 238000007710 freezing Methods 0.000 claims abstract description 26
- 230000008014 freezing Effects 0.000 claims abstract description 26
- 239000007788 liquid Substances 0.000 claims description 19
- 239000000463 material Substances 0.000 claims description 13
- 238000007789 sealing Methods 0.000 claims description 7
- 229920000936 Agarose Polymers 0.000 claims description 4
- 229910017053 inorganic salt Inorganic materials 0.000 claims description 4
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 4
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 8
- 239000012528 membrane Substances 0.000 abstract description 7
- 238000004321 preservation Methods 0.000 description 12
- 238000003780 insertion Methods 0.000 description 5
- 230000037431 insertion Effects 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
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Abstract
An assembled wet-turning cooling box for an immunoblotting test comprises a bottom plate and four side plates; the bottom plate and the side plates are of closed rectangular structures with hollow accommodating cavities inside, a first jack is arranged on the inner side of each edge of the bottom plate and close to the edge, and a first feeding hole and a first one-way exhaust valve are arranged on the top surface of the bottom plate; the bottom of each side plate is provided with a first inserting lug, the first inserting lug is installed in a first jack of the bottom plate, one side of each side plate is provided with a second inserting lug, the other side of each side plate is provided with a second jack, the second inserting lug is used for being inserted into a second jack of the adjacent side plate, and the four side plates and the bottom plate are assembled to form an open rectangular groove body; a second feeding hole and a second one-way exhaust valve are arranged at the position, close to the top end, of the outer side of the side plate; the hollow accommodating cavities of the side plates and the bottom plate are filled with freezing and cooling blocks. This cooling box simple structure, repeatedly usable can continuously be refrigerated at the wet in-process that changes to the effect of membrane is changeed in the assurance.
Description
Technical Field
The utility model belongs to the field of cooling devices, and particularly relates to an assembled wet-turning cooling box for an immunoblot test.
Background
In the Western Blot experiment, ice bath is needed for wet rotation, a sandwich method of filter paper-gel-membrane-filter paper is generally adopted during wet rotation, the gel is close to the negative electrode, and the membrane is close to the positive electrode. The protein is combined with SDS, so that the protein is negatively charged, and can move from a negative electrode to a positive electrode under the action of current so as to be transferred to a membrane, a large amount of heat can be released in the process of transferring the membrane, and the temperature is reduced by using an ice bath so as to prevent poor effect of transferring the membrane. The existing ice blocks are used, a large number of ice blocks are needed each time, the ice blocks are directly discarded after being melted, and great waste exists in cost and resource utilization.
Disclosure of Invention
In view of the above technical problems and disadvantages, an object of the present invention is to provide a split mounting type wet-to-wet cooling box for immunoblotting tests, which has a simple structure, can be reused, and can be continuously cooled during wet-to-wet process to ensure the effect of membrane transfer.
In order to realize the purpose, the utility model is realized by adopting the following technical scheme:
an assembled wet-turning cooling box for an immunoblotting test comprises a cooling box main body, wherein the cooling box main body comprises a bottom plate and four side plates; the bottom plate is of a closed rectangular structure with a hollow accommodating cavity inside, a first jack is arranged on the inner side of each edge of the bottom plate and close to the edge, a first feed hole and a first one-way exhaust valve are arranged on the top surface of the bottom plate, and a sealing cover is arranged on the first feed hole; the four side plates are respectively arranged on four sides of the bottom plate, the side plates are of a closed rectangular structure with a hollow accommodating cavity inside, the bottom of each side plate is provided with a first inserting lug, the first inserting lug is arranged in a first inserting hole of the bottom plate, one side of each side plate is provided with a second inserting lug, the other side of each side plate is provided with a second inserting hole, the second inserting lug is used for being inserted into a second inserting hole of the adjacent side plate, and the four side plates and the bottom plate are assembled to form an open rectangular groove body; a second feeding hole and a second one-way exhaust valve are arranged at the position, close to the top end, of the outer side of the side plate, and a sealing cover is arranged on the second feeding hole; the cavity of curb plate and bottom plate holds the intracavity and is filled with freezing cooling piece, freezing cooling piece is that liquid cooling material forms after freezing.
Preferably, the cooling box further comprises a heat-insulating shell, and the rectangular groove body formed by splicing the bottom plate and the four side plates is placed in the heat-insulating shell.
Preferably, the bottom plate and the four side plates are made of PE materials, and the freezing and cooling block is formed by mixing inorganic salt, sodium polyacrylate, agarose and water in a ratio of 30:2:9:100 and freezing.
Preferably, the bottom plate and the side plate are both provided with liquid perspective windows, and the quantity of liquid cooling materials in the hollow accommodating cavities of the bottom plate and the side plate can be observed conveniently through the liquid perspective windows.
Preferably, the heat preservation shell is provided with a heat preservation cover, the two sides of the heat preservation shell are provided with the hand straps, and the frozen cooling box main body is convenient to transport through the heat preservation shell.
The utility model has the advantages and beneficial effects that:
1. the cooling box provided by the utility model adopts an assembly form, and can be disassembled and respectively placed in a refrigerator at the temperature of-20 ℃ for freezing when a liquid cooling material needs to be frozen, so that the freezing process is convenient and does not occupy space.
2. The one-way exhaust valves are arranged on the bottom plate and the side plate of the cooling box provided by the utility model, so that the bottom plate or the side plate can be prevented from being expanded and deformed due to liquid expansion in the freezing process, and the frozen bottom plate and the frozen side plate are kept flat due to the arrangement of the one-way exhaust valves, thereby ensuring the film transferring effect.
3. The freezing and cooling block filled in the cooling box is formed by mixing inorganic salt, sodium polyacrylate, agarose and water in a ratio of 30:2:9:100 and then freezing, the cold storage time of the freezing and cooling block at room temperature can reach 5-7 hours, the cooling effect is better, the freezing and cooling block can be continuously cooled in the wet-turning process, and the film-turning effect is ensured; in addition, the frozen cooling blocks are still stored in the bottom plate and the side plates after being melted, and can be reused for tens of times after being frozen, so that the resource waste is reduced, and the cost is reduced.
4. The cooling box provided by the utility model can be placed in the heat preservation shell when in use, and the arrangement of the heat preservation shell can further prolong the cold accumulation duration of the freezing cooling block.
5. The cooling box provided by the utility model is provided with the liquid perspective window, so that the quantity of the cooling material injected into the side plate and the bottom plate can be observed conveniently through the liquid perspective window, and the side plate and the bottom plate can be prevented from being broken by expansion after the cooling material is filled and is frozen too much.
Drawings
FIG. 1 is a schematic structural view of a cooling box according to the present invention;
FIG. 2 is a schematic structural view of the base plate of the present invention;
FIG. 3 is a schematic view of the construction of the side panel of the present invention;
fig. 4 is a cross-sectional view of the base plate of the present invention.
Description of the drawings: the cooling box comprises a cooling box main body 1, a heat preservation shell 2, a liquid perspective window 3, a freezing cooling block 4, a bottom plate 11, a side plate 12, a first insertion hole 111, a first feeding hole 112, a first one-way exhaust valve 113, a first insertion lug 121, a second insertion lug 122, a second insertion hole 123, a second feeding hole 124 and a second one-way exhaust valve 125.
Detailed Description
The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
In the description of the present application, it is to be noted that the terms "inside", "below", and the like refer to orientations or positional relationships based on orientations or positional relationships shown in the drawings or orientations or positional relationships that are conventionally arranged when products of the application are used, and are used only for convenience in describing the application and simplifying the description, but do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the application. Furthermore, the terms "first," "second," and the like are used merely to distinguish one description from another, and are not to be construed as indicating or implying relative importance.
In the description of the present application, it is also to be noted that, unless otherwise explicitly specified or limited, the terms "disposed" and "connected" are to be interpreted broadly, e.g., as being either fixedly connected, detachably connected, or integrally connected; they may be mechanically coupled, directly coupled, indirectly coupled through intervening media, or may be interconnected between two elements. The specific meaning of the above terms in the present application can be understood in a specific case by those of ordinary skill in the art.
As shown in fig. 1 to 4, the present invention provides an assembly type wet-to-wet cooling box for an immunoblotting test, which comprises a cooling box main body 1 and a heat preservation shell 2; the cooling box main body 1 comprises a bottom plate 11 and four side plates 12, the bottom plate 11 and the four side plates 12 are made of PE materials, and a rectangular groove body formed by assembling the bottom plate 11 and the four side plates 12 is placed in the heat-insulating shell 2; the bottom plate 11 is a closed rectangular structure with a hollow accommodating cavity inside, a first insertion hole 111 is formed in the inner side of each edge of the bottom plate 11 and close to the edge, a first feeding hole 112 and a first one-way exhaust valve 113 are formed in the top surface of the bottom plate, and a sealing cover is arranged on the first feeding hole; the four side plates 12 are respectively installed on four sides of the bottom plate 11, each side plate 12 is a closed rectangular structure with a hollow accommodating cavity inside, a first inserting convex block 121 is arranged at the bottom of each side plate 12, the first inserting convex block 121 is installed in a first inserting hole 111 of the bottom plate, a second inserting convex block 122 is arranged on one side of each side plate 12, a second inserting hole 123 is arranged on the other side of each side plate 12, the second inserting convex blocks 122 are used for being inserted into second inserting holes of the adjacent side plates, and the four side plates and the bottom plate are assembled to form an open rectangular groove body; a second feeding hole 124 and a second one-way exhaust valve 125 are arranged on the outer side of the side plate at a position close to the top end, and a sealing cover is arranged on the second feeding hole 124; the hollow accommodating cavities of the side plates and the bottom plate are filled with freezing and cooling blocks 4, and the freezing and cooling blocks 4 are formed by freezing liquid cooling materials (inorganic salt, sodium polyacrylate, agarose and water are mixed in a ratio of 30:2:9: 100); all be provided with liquid perspective window 3 on bottom plate 11 and the curb plate 12, be convenient for observe the volume that bottom plate and curb plate cavity hold intracavity liquid cooling material through liquid perspective window 3.
Furthermore, be provided with the heat preservation lid on the heat preservation casing, the both sides of heat preservation casing are provided with portable area, conveniently transport refrigerated bottom plate and curb plate through the heat preservation casing.
The use method of the cooling box comprises the following steps:
injecting a liquid cooling material into the bottom plate and the side plate through the feeding hole, and covering the second feeding hole by using a sealing cover after the liquid cooling material reaches the position which can be observed by the liquid perspective window 3; then, the bottom plate and the side plates are put in a refrigerator at the temperature of minus 20 ℃ for freezing, after freezing, the bottom plate and the side plates are put in a heat insulation shell after being assembled, then, the wet-turning groove is put in the cooling box main body 1, and a sandwich clip and a transfer liquid are placed in the wet-turning groove for carrying out an experiment; after the experiment, can directly put the box main part of cooling in the refrigerator freezing according to the refrigerator inner space condition, also can unpack it apart the back and freeze, the reuse rate and the cooling effect of this cooling box are better, can continuously be refrigerated at the wet commentaries on classics in-process to the effect of guaranteeing to change the membrane.
The above description is only a preferred embodiment of the present application and is not intended to limit the present application.
Claims (5)
1. An assembled wet-turning cooling box for an immunoblotting test comprises a cooling box main body, wherein the cooling box main body comprises a bottom plate and four side plates; the device is characterized in that the bottom plate is of a closed rectangular structure with a hollow accommodating cavity inside, a first jack is arranged on the inner side of each edge of the bottom plate and close to the edge, a first feeding hole and a first one-way exhaust valve are arranged on the top surface of the bottom plate, and a sealing cover is arranged on the first feeding hole; the four side plates are respectively arranged on four sides of the bottom plate, the side plates are of a closed rectangular structure with a hollow accommodating cavity inside, the bottom of each side plate is provided with a first inserting lug, the first inserting lug is arranged in a first inserting hole of the bottom plate, one side of each side plate is provided with a second inserting lug, the other side of each side plate is provided with a second inserting hole, the second inserting lug is used for being inserted into a second inserting hole of the adjacent side plate, and the four side plates and the bottom plate are assembled to form an open rectangular groove body; a second feeding hole and a second one-way exhaust valve are arranged at the position, close to the top end, of the outer side of the side plate, and a sealing cover is arranged on the second feeding hole; the cavity of curb plate and bottom plate holds the intracavity and is filled with freezing cooling piece, freezing cooling piece is that liquid cooling material forms after freezing.
2. The assembly type wet-turning cooling box for the immunoblotting test according to claim 1, wherein the cooling box further comprises a heat-insulating shell, and the rectangular groove body formed by assembling the bottom plate and the four side plates is placed in the heat-insulating shell.
3. The assembly type wet-turning cooling box for the immunoblotting test according to claim 1 or 2, wherein the bottom plate and the four side plates are made of PE materials, and the freezing cooling block is formed by mixing inorganic salt, sodium polyacrylate, agarose and water in a ratio of 30:2:9:100 and freezing.
4. The assembling type wet-turning cooling box for the immunoblotting test according to claim 1 or 2, wherein the bottom plate and the side plate are both provided with liquid perspective windows.
5. The split mounting type wet-turning cooling box for the immunoblotting test according to claim 2, wherein a heat-insulating cover is arranged on the heat-insulating shell, and hand-held belts are arranged on two sides of the heat-insulating shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122004754.2U CN215708639U (en) | 2021-08-23 | 2021-08-23 | Split mounting type wet-turning cooling box for immunoblotting test |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122004754.2U CN215708639U (en) | 2021-08-23 | 2021-08-23 | Split mounting type wet-turning cooling box for immunoblotting test |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215708639U true CN215708639U (en) | 2022-02-01 |
Family
ID=80006564
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122004754.2U Active CN215708639U (en) | 2021-08-23 | 2021-08-23 | Split mounting type wet-turning cooling box for immunoblotting test |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215708639U (en) |
-
2021
- 2021-08-23 CN CN202122004754.2U patent/CN215708639U/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Assembled wet cooling box for immunoblotting test Effective date of registration: 20221017 Granted publication date: 20220201 Pledgee: Industrial Bank Co.,Ltd. Changchun Branch Pledgor: CHANGCHUN WEISHI DETECTION TECHNOLOGY SERVICE Co.,Ltd. Registration number: Y2022220000088 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20231031 Granted publication date: 20220201 Pledgee: Industrial Bank Co.,Ltd. Changchun Branch Pledgor: CHANGCHUN WEISHI DETECTION TECHNOLOGY SERVICE Co.,Ltd. Registration number: Y2022220000088 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: Assembled wet to cool box for immunoblotting test Effective date of registration: 20231101 Granted publication date: 20220201 Pledgee: Industrial Bank Co.,Ltd. Changchun Branch Pledgor: CHANGCHUN WEISHI DETECTION TECHNOLOGY SERVICE Co.,Ltd. Registration number: Y2023220000114 |