CN217136618U

CN217136618U - Gradient cooling box for cell cryopreservation bag

Info

Publication number: CN217136618U
Application number: CN202220884840.9U
Authority: CN
Inventors: 肖震; 赵娜; 周健; 黄帅
Original assignee: Medixin Tianjin Biotechnology Co ltd
Current assignee: Medixin Tianjin Biotechnology Co ltd
Priority date: 2022-04-18
Filing date: 2022-04-18
Publication date: 2022-08-09
Anticipated expiration: 2032-04-18

Abstract

The utility model discloses a gradient cooling box to bag is deposited to cell freezing belongs to cell freezing and deposits the storage technology field, including cooling box main part, the recess, elasticity part, grip block and lid, cooling box main part is the cavity structure, and is full of isopropyl alcohol in the cavity of cooling box main part, has seted up a plurality of recesses in the cooling box main part, all is equipped with elasticity part and grip block in a plurality of recesses, elasticity part is equipped with a plurality ofly, the one end of a plurality of elasticity parts and the interior wall connection of recess, the other end of a plurality of elasticity parts and the first lateral wall of grip block are connected, be equipped with accommodation space between the second lateral wall of grip block and the inner wall of recess, cooling box main part upper cover is equipped with the lid. This application is put into accommodation space with the freezing bag of cell, makes the grip block to the freezing bag direction extrusion of cell, and the freezing bag of cell is by the centre gripping between the lateral wall of grip block and recess to make the freezing bag of cell keep vertical state and closely paste tightly with the interior isopropyl alcohol of cooling box main part, improve cell survival rate.

Description

Gradient cooling box for cell cryopreservation bag

Technical Field

The utility model relates to a technical field, concretely relates to gradient cooling box to cell cryopreserving bag is stored to cell refrigeration.

Background

Cryopreservation of cells is a routine task in cell culture. Compared with cell passage preservation, the cell freezing can reduce manpower and expenditure, reduce pollution, reduce the change of cell genetic characters and delay aging. When the cell cools below zero, the organelles dehydrate, the concentration of soluble material in the cell increases, and ice crystals form within the cell. The ice crystals have different influences on cells, and the large ice crystals easily cause damage and rupture of cell membranes and organelles, so that the survival rate, the state and the like of the cells revived by the ice crystals are far different from the state before freezing. In order to reduce the formation of ice crystals, slow cooling is required during cell freezing storage.

The basic application program cooling instrument in the market is completed, the manufacturing cost and the running cost of the program cooling instrument are high, a large amount of liquid nitrogen is needed, and a large amount of time cost of workers is consumed. Most of the gradient cooling boxes can only freeze tubular cells and cannot freeze cells filled in cell bags.

Therefore, it is an urgent technical problem to be solved by those skilled in the art to provide a novel cooling box to enable the cell freezing bag to be placed therein and to be tightly attached in the cooling box, so as to improve the freezing survival rate of cells and reduce the manufacturing cost.

SUMMERY OF THE UTILITY MODEL

Therefore, the utility model provides a gradient cooling box to cell cryopreserving bag to solve among the prior art because adopt the procedure cooling appearance to freeze the problem that the cost of manufacture is high that leads to the cell.

In order to achieve the above object, the present invention provides the following technical solutions:

the utility model provides a gradient cooling box to bag is deposited to cell freezing, includes cooling box main part, recess, elasticity part, grip block and lid, cooling box main part is the cavity structure, just be full of isopropanol in the cavity of cooling box main part, it is a plurality of to have seted up in the cooling box main part the recess is, and is a plurality of all be equipped with in the recess elasticity part and grip block, the elasticity part is equipped with a plurality ofly, and is a plurality of the one end of elasticity part with the interior wall connection of recess, it is a plurality of the other end of elasticity part with the first lateral wall connection of grip block, the second lateral wall of grip block with be equipped with accommodation space between the inner wall of recess, cooling box main part upper cover is equipped with the lid.

Furthermore, the cell freezing bag comprises positioning columns, wherein the positioning columns are arranged at four corners of the clamping plate, and the four corners of the cell freezing bag are fixed at the four corners of the clamping plate through the positioning columns.

Further, the clamping plate is a reticulated foam plate.

Further, still including annotating liquid mouth and sealed lid, seted up in the cooling box main part annotate the liquid mouth, just it is equipped with to annotate liquid mouth upper cover sealed lid.

Further, still include extension plate and rubber block, the both sides downwardly extending of lid is equipped with the extension plate, be equipped with the rubber block on the inside wall of extension plate, just the rubber block extrude in between extension plate and the cooling box main part.

Further, still include the handle, the up end of lid is equipped with the handle.

Further, the cooling box main body is an aviation foam board.

Further, the elastic component is a spring.

The utility model has the advantages of as follows:

this application has seted up the recess in cooling box main part, is equipped with elasticity part and grip block in the recess, and elasticity part one end and the interior wall connection of recess, the other end of elasticity part and the first lateral wall of grip block are connected, are equipped with accommodation space between the second lateral wall of grip block and the lateral wall of recess. Put into accommodation space with cell freezing bag, the elastic component receives the extrusion, makes the grip block extrude to cell freezing bag direction, and cell freezing bag is by the centre gripping between the lateral wall of grip block and recess to make cell freezing bag keep vertical state, avoid cell freezing bag pile to influence cell survival rate. And the cell freezing bag is extruded to be in close contact with the inner wall of the cooling box main body, so that the cell freezing bag is tightly attached to isopropanol in the cooling box main body, and the survival rate of cells is improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.

The structure, ratio, size and the like shown in the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by people familiar with the technology, and are not used for limiting the limit conditions which can be implemented by the present invention, so that the present invention has no technical essential significance, and any structure modification, ratio relationship change or size adjustment should still fall within the scope which can be covered by the technical content disclosed by the present invention without affecting the efficacy and the achievable purpose of the present invention.

Fig. 1 is a schematic exploded view of the present invention;

FIG. 2 is an enlarged view of a portion of FIG. 1 at A;

FIG. 3 is a cross-sectional view of the cooling cassette body;

FIG. 4 is a schematic structural view of the cooling box main body covered by the cover body;

in the figure:

1, cooling a box main body; 2, grooves; 3 an elastic member; 4, clamping a plate; 5, a cover body; 6, positioning columns; 7, a liquid injection port; 8, sealing the cover; 9, extending plates; 10 a rubber block; 11 a handle.

Detailed Description

The present invention is described in terms of specific embodiments, and other advantages and benefits of the present invention will become apparent to those skilled in the art from the following disclosure. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In order to solve the related technical problems in the prior art, an embodiment of the present application provides a gradient cooling box for a cell cryopreservation bag, which aims to solve the problems that the existing gradient cooling box cannot accommodate a cell freezing bag, and the like, and achieve the effect of accommodating the cell freezing bag, and specifically includes a cooling box main body 1, a groove 2, an elastic component 3, a clamping plate 4 and a cover body 5, as shown in fig. 1-4. As shown in fig. 3, the cooling box body 1 is a cavity structure, and the cavity of the cooling box body 1 is filled with isopropyl alcohol. The isopropanol is a commonly used antifreezing agent for cell freezing, so that the cells in the cooling box main body 1 are prevented from being rapidly cooled, the formation of ice crystals is reduced, the cells are protected from being damaged, and the survival rate of the cells is improved.

As shown in figure 1, a liquid injection port 7 is arranged on the cooling box main body 1, and a sealing cover 8 is arranged on the liquid injection port 7 in a covering manner. The sealing cover 8 is opened to expose the liquid injection port 7, and isopropanol is injected into the liquid injection port 7, so that the cavity of the cooling box main body 1 is filled with the isopropanol. Meanwhile, the liquid injection port 7 is formed, so that when the isopropanol volatilizes, the isopropanol can be conveniently added into the cooling box main body 1. Isopropanol is added into the cooling box main body 1, and when the cell freezing bag is placed into the cooling box main body 1, the cell freezing bag is wrapped by the isopropanol. After the cooling box main body 1 is placed in an ultra-low temperature refrigerator at minus 80 ℃, the temperature of the cell freezing bag can realize gradient cooling at 1 ℃ per minute, the survival rate of cells can be greatly improved, and ice crystals generated by rapid cooling of the cell freezing bag are avoided. And the cooling box main body 1 is an aviation foam board, and the aviation foam board also has a heat preservation effect and can prevent the cell freezing bag from being cooled rapidly. When a beginner forgets to check whether the isopropanol remains in the cooling box main body 1, the cooling box main body 1 can also continuously maintain the gradient cooling of the cell freezing bag, so that the cells are protected from being damaged, massive cell death is avoided, and the survival rate of the cells is further improved.

As shown in fig. 1 or 3, a plurality of grooves 2 are formed in the cooling box body 1, elastic members 3 and clamping plates 4 are arranged in the grooves 2, and the elastic members 3 are springs and can be extruded and have resilience performance. Elastic component 3 is equipped with a plurality ofly, and the one end of a plurality of elastic component 3 and the inner wall connection of recess 2, the other end of a plurality of elastic component 3 and the first lateral wall of grip block 4 are connected, are equipped with accommodation space between the second lateral wall of grip block 4 and the lateral wall of recess 2. The containing space is slightly smaller than the width of the cell freezing bag, so that after the cell freezing bag is placed in the containing space, the clamping plate 4 is extruded, the clamping plate 4 extrudes the elastic component 3, and the elastic component 3 has rebounded potential energy. The elastic member 3 rebounds to press the holding plate 4 again, so that the holding plate 4 presses the cell freezing bag, thereby clamping the cell freezing bag between the holding plate 4 and the side wall of the groove 2.

During the use, put into the cell freezing bag the accommodation space between the second lateral wall of grip block 4 and the lateral wall of recess 2, elastic component 3 receives the extrusion, makes grip block 4 to the extrusion of cell freezing bag direction, and the cell freezing bag is by the centre gripping between the lateral wall of grip block 4 and recess 2 to make the cell freezing bag keep vertical state, avoid the cell freezing bag to pile up and influence the cell survival rate. And the cell freezing bag is extruded to be in close contact with the inner wall of the cooling box main body 1, so that the cell freezing bag is tightly attached to the isopropanol in the cooling box main body 1, and the survival rate of cells is improved.

As shown in FIG. 2 or 3, positioning posts 6 are disposed at four corners of the holding plate 4, and holes are correspondingly disposed at four corners of the cell freezing bag. When the cell freezing bag is placed into the groove 2 of the cooling box main body 1, the perforation holes at the four corners of the cell freezing bag are hung on the positioning columns 6 at the four corners of the clamping plate 4, so that the cell freezing bag is fixed in position, the sliding caused by the movement of the cell freezing bag is reduced, and the stability is improved. The cell freezing bag is kept in a vertical state, so that the cell survival rate is prevented from being influenced by the accumulation of the cell freezing bag.

The holding plate 4 is a net-shaped foam plate, and as shown in fig. 3, the holding plate 4 has a net-shaped structure. When the cell freezing bag is clamped between the second side wall of the clamping plate 4 and the side wall of the groove 2, the first side wall of the cell freezing bag is tightly attached to the side wall of the groove 2, so that the cell freezing bag is tightly attached to isopropanol in the cooling box main body 1, gradient cooling is realized, and the survival rate of cells is improved; the second lateral wall of cell freezing bag closely laminates with the second lateral wall of grip block 4, on the one hand, can make the cell freezing bag that is extrudeed keep vertical state's stability, and on the other hand, exposes on the second lateral wall of cell freezing bag and the face that netted board contacted, conveniently carries out the heat exchange with the cold air contact in the space.

As shown in fig. 4, the cover body 5 is covered on the cooling box body 1, and the handle 11 is arranged on the upper end surface of the cover body 5, so that an operator can take the cover body 5 conveniently. As shown in fig. 1, extension plates 9 extend downwards from two sides of the cover 5, rubber blocks 10 are arranged on the inner side walls of the extension plates 9, and the rubber blocks 10 are extruded between the extension plates 9 and the cooling box body 1. When a cell freezing bag is placed in the cooling box main body 1, the handle 11 is held by hand, the cover body 5 is pressed downwards, and the cover body 5 is covered on the cooling box main body 1. At this moment, the extension plates 9 extending downwards from the two sides of the cover body 5 contact with the two sides of the cooling box main body 1, and the rubber blocks 10 on the inner side surfaces of the extension plates 9 are extruded, so that the extension plates 9 and the cover body 5 are tightly attached to the cooling box main body 1, and the cover body 5 is covered. Then the covered cooling box main body 1 is put into an ultra-low temperature refrigerator for gradient cooling; when the cell condition and the residual condition of isopropanol in the cooling box main body 1 need to be checked, the handle 11 is held by hand, the cover body 5 is pulled upwards, and the cover body 5 is taken down from the cooling box main body 1. At this time, the rubber block 10 on the inner side surface of the extension plate 9 is restored to its original shape.

The utility model discloses use as follows:

the cell freezing bag is placed in the accommodating space between the second side wall of the holding plate 4 and the side wall of the groove 2, the elastic component 3 is extruded, the holding plate 4 is extruded towards the cell freezing bag, and the cell freezing bag is held between the holding plate 4 and the side wall of the groove 2. After the cell freezing bag is placed in the cooling box body 1, the handle 11 is held by hand, the cover body 5 is pressed downwards, and the cover body 5 is covered on the cooling box body 1. At this moment, the extension plates 9 extending downwards from the two sides of the cover body 5 contact with the two sides of the cooling box main body 1, and the rubber blocks 10 on the inner side surfaces of the extension plates 9 are extruded, so that the extension plates 9 and the cover body 5 are tightly attached to the cooling box main body 1, and the cover body 5 is covered. And then the covered cooling box main body 1 is put into an ultra-low temperature refrigerator for gradient cooling.

Although the invention has been described in detail with respect to the general description and the specific embodiments, it will be apparent to those skilled in the art that modifications and improvements can be made based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.

Claims

1. The gradient cooling box for the cell cryopreservation bag is characterized by comprising a cooling box main body (1), grooves (2), elastic parts (3), a clamping plate (4) and a cover body (5), wherein the cooling box main body (1) is of a cavity structure, the cavity of the cooling box main body (1) is filled with isopropanol, a plurality of grooves (2) are formed in the cooling box main body (1), the elastic parts (3) and the clamping plate (4) are arranged in the grooves (2), the elastic parts (3) are arranged in a plurality of numbers, one ends of the elastic parts (3) are connected with the inner wall of the grooves (2), the other ends of the elastic parts (3) are connected with the first side wall of the clamping plate (4), and an accommodating space is formed between the second side wall of the clamping plate (4) and the inner wall of the grooves (2), the cover body (5) is arranged on the upper cover of the cooling box main body (1).

2. The gradient cooling box for the cell freezing bag according to claim 1, further comprising positioning pillars (6), wherein the positioning pillars (6) are disposed at four corners of the holding plate (4), and the four corners of the cell freezing bag are fixed at the four corners of the holding plate (4) through the positioning pillars (6).

3. The gradient cooling box for the cell cryopreservation bag according to claim 1, wherein the holding plate (4) is a reticulated foam plate.

4. The gradient cooling box for the cell freezing bag according to claim 1, further comprising a liquid injection port (7) and a sealing cover (8), wherein the liquid injection port (7) is opened on the cooling box main body (1), and the sealing cover (8) is covered on the liquid injection port (7).

5. The gradient cooling box for the cell cryopreservation bag according to claim 1, further comprising an extension plate (9) and a rubber block (10), wherein the extension plate (9) extends downwards from two sides of the cover body (5), the rubber block (10) is arranged on the inner side wall of the extension plate (9), and the rubber block (10) is extruded between the extension plate (9) and the cooling box body (1).

6. The gradient cooling box for the cell freezing bag according to claim 1, further comprising a handle (11), wherein the handle (11) is arranged on the upper end face of the cover body (5).

7. The gradient cooling box for the cell cryopreservation bag according to claim 1, wherein the cooling box body (1) is an aviation foam plate.

8. The gradient cooling box for the cell cryopreservation bag according to claim 1, wherein the elastic member (3) is a spring.