CN220343384U - Cell cryopreserving device with high cell viability - Google Patents
Cell cryopreserving device with high cell viability Download PDFInfo
- Publication number
- CN220343384U CN220343384U CN202322012717.5U CN202322012717U CN220343384U CN 220343384 U CN220343384 U CN 220343384U CN 202322012717 U CN202322012717 U CN 202322012717U CN 220343384 U CN220343384 U CN 220343384U
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- Prior art keywords
- chamber
- sealing plate
- freezing
- thawing
- preservation
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- 230000003833 cell viability Effects 0.000 title claims abstract description 13
- 238000007710 freezing Methods 0.000 claims abstract description 48
- 230000008014 freezing Effects 0.000 claims abstract description 48
- 238000007789 sealing Methods 0.000 claims abstract description 46
- 238000010257 thawing Methods 0.000 claims abstract description 32
- 238000004321 preservation Methods 0.000 claims abstract description 24
- 239000000463 material Substances 0.000 claims abstract description 23
- 238000005138 cryopreservation Methods 0.000 claims abstract description 18
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 22
- 239000007788 liquid Substances 0.000 claims description 11
- 229910052757 nitrogen Inorganic materials 0.000 claims description 11
- 230000000903 blocking effect Effects 0.000 claims description 3
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 4
- 210000003205 muscle Anatomy 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The utility model discloses a cell freezing device with high cell viability, which is internally provided with a freezing chamber and a thawing chamber, wherein a baffle rib is fixed on the inner wall of the periphery of the preservation chamber, a hole formed by the inner end surface of the baffle rib forms an inner cavity opening of the thawing chamber, a switch plate is inserted into and penetrates through the side wall of the periphery of the preservation chamber, a hole formed by the inner end surface of the switch plate forms an inner cavity opening of the freezing chamber, a material taking rod penetrates into the thawing chamber from the left side of the preservation chamber and extends into the freezing chamber, a first sealing plate is abutted against the left end surface of the baffle rib and seals the inner cavity opening of the thawing chamber, a second sealing plate is abutted against the left end surface of the switch plate and seals the inner cavity opening of the thawing chamber, and a sample container is fixed on the material taking rod and is arranged in the freezing chamber. The utility model has reasonable structural design, is suitable for temporary cell cryopreservation, can meet the requirement of temporary cell cryopreservation by arranging a small cryopreservation chamber, has low use cost, is very convenient for cell taking, and has good use effect.
Description
Technical Field
The utility model relates to a cell cryopreservation device with high cell viability.
Background
When the existing cells are frozen, the cells are placed in a liquid nitrogen tank. When the cells are temporarily frozen, if the cells are still stored by means of a liquid nitrogen tank, the problem of 'small and big do' exists, and the use cost of the professional liquid nitrogen tank is high. The existing laboratory also can make simple temporary freezing structure, namely, a plastic water barrel is arranged, then liquid nitrogen is introduced into the water barrel, but the cell taking of the water barrel structure is quite unchanged, and the use effect is poor.
Disclosure of Invention
The utility model aims to solve the problems in the prior art and provides a cell cryopreservation device with high cell viability.
The utility model adopts the technical scheme that:
the utility model provides a cell cryopreserving device of high cell activity rate, includes the preserving chamber, gets material pole, sample container, switch board, first closing plate, second closing plate, third closing plate and fourth closing plate, be equipped with in the preserving chamber and freeze the preserving chamber and break down the freezing chamber, be fixed with on the inner wall all around of preserving chamber and keep off the muscle, the hole that the terminal surface encloses forms the inner chamber mouth of thawing the freezing chamber in keeping off the muscle, the switch board is pegged graft and is run through in the lateral wall all around of preserving chamber, the hole that the terminal surface encloses in the switch board forms the inner chamber mouth of freezing the preserving chamber, get material pole and penetrate the freezing chamber by the left side of preserving chamber and stretch into in the freezing chamber, first closing plate, second closing plate, third closing plate and fourth closing plate are fixed in proper order on getting material pole, first closing plate is contradicted on the left side terminal surface of keeping off the muscle and will break down the inner chamber mouth of freezing chamber, the second is contradicted on the left side terminal surface of switch board and will freeze the inner chamber and seal down, sample container is fixed on getting material pole and is put in the freezing the preserving chamber.
Further, after the first sealing plate seals the thawing chamber and the second sealing plate seals the freezing chamber, a buffer chamber is formed between the sealed thawing chamber and the sealed freezing chamber.
Further, the switch plate is pulled outwards to expand the inner cavity opening of the freezing chamber, the material taking rod moves rightwards to enable the sample container to be placed in the freezing chamber, the third sealing plate abuts against the right side end face of the blocking rib and seals the inner cavity opening of the freezing chamber, and the fourth sealing plate abuts against the right side end face of the switch plate and seals the inner cavity opening of the freezing chamber after expansion.
Further, the preservation chamber is provided with a feeding port and a material taking port, and the feeding port and the material taking port are respectively communicated with the freezing chamber and the thawing chamber correspondingly.
Further, the freezing storage chamber is frozen by liquid nitrogen, and a liquid nitrogen access pipe is arranged on the outer wall of the storage chamber.
Further, the area of the fourth sealing plate is larger than that of the first sealing plate, the area of the first sealing plate is larger than that of the third sealing plate, and the area of the third sealing plate is larger than that of the second sealing plate.
Further, the switch board is provided with a flange board which is fixedly connected with the outer wall surface of the preservation chamber.
The utility model has the following beneficial effects:
the utility model has reasonable structural design, is suitable for temporary cell cryopreservation, can meet the requirement of temporary cell cryopreservation by arranging a small cryopreservation chamber, has low use cost, is very convenient for cell taking, and has good use effect.
Drawings
Fig. 1 is a structural diagram of the present utility model.
Fig. 2 is a view showing a structure in which a sample container is placed in a thawing chamber.
Description of the embodiments
The utility model is further described below with reference to the accompanying drawings.
Referring to fig. 1 and 2, the cell cryopreservation apparatus with high cell viability according to the present utility model includes a preservation chamber 1, a material taking rod 2, a sample container 3, a switching plate 4, a first sealing plate 51, a second sealing plate 52, a third sealing plate 53, and a fourth sealing plate 54.
The preservation room 1 is internally provided with a freezing preservation room 11 and a thawing room 13, the inner wall of the periphery of the preservation room 1 is fixedly provided with a baffle rib 10, and a hole formed by the inner end surface of the baffle rib 10 forms a cavity opening of the thawing room 13.
The switch board 4 is inserted and penetrated on the peripheral side wall of the preservation chamber 1, and the hole formed by the inner end surface of the switch board 4 forms the inner cavity opening of the freezing chamber 11.
The material taking rod 2 penetrates into the thawing chamber 13 from the left side of the preservation chamber 1 and extends into the freezing chamber 11, a first sealing plate 51, a second sealing plate 52, a third sealing plate 53 and a fourth sealing plate 54 are sequentially fixed on the material taking rod 2,
the first sealing plate 51 abuts against the left end face of the rib 10 and closes the inner cavity opening of the thawing chamber 13. The second sealing plate 52 abuts against the left end face of the switch plate 4 and seals the inner cavity opening of the freezing chamber 11, and the sample container 3 is fixed on the material taking rod 2 and placed in the freezing chamber 11.
The preserving chamber 1 is provided with a feeding port 61 and a material taking port 62, and the feeding port 61 and the material taking port 62 are respectively communicated with the freezing chamber 11 and the thawing chamber 13 correspondingly. The freezing chamber 11 is frozen by liquid nitrogen, and a liquid nitrogen access pipe is arranged on the outer wall of the preservation chamber 1.
When freezing the cell sample, the cell sample is placed in the sample container 3 by opening the inlet 61, and the cell sample is stored in the closed freezing chamber 11 by freezing because the structure is as shown in fig. 1.
When the cell sample needs to be thawed, the switch board 4 is drawn outwards and the inner cavity opening of the freezing chamber is enlarged, then the material taking rod 2 moves rightwards to enable the sample container 3 to be placed in the thawing chamber 13, the third sealing plate 53 abuts against the right side end face of the blocking rib 10 and seals the inner cavity opening of the thawing chamber 13, and the fourth sealing plate 54 abuts against the right side end face of the switch board 4 and seals the inner cavity opening of the freezing chamber after the expansion, as shown in fig. 2. And after the cell thawing is finished, the material taking opening 62 is opened, and the cell sample is taken out.
In order to realize that the sample container 3 is in a preservation closed state in the process of switching the freezing chamber 11 and the thawing chamber 13 back and forth, the freezing chamber 11 and the thawing chamber 13 are in a structural design, namely the drawable switch board 4 and four sealing boards with different areas, so that liquid nitrogen in the freezing chamber is not leaked in the thawing process due to the ingenious structural design.
After the first sealing plate 51 seals the thawing chamber 13 and the second sealing plate 52 seals the cryopreservation chamber 11, a buffer chamber 12 is formed between the sealed thawing chamber 13 and the sealed cryopreservation chamber 11. The presence of the buffer chamber 12 is such that there is no direct adjacency between the freezer compartment 11 and the thawing compartment 13, reducing the heat transfer between the freezer compartment 11 and the thawing compartment 13.
The switch plate 4 is provided with a flange plate which is fixedly connected with the outer wall surface of the preservation chamber 1. When the switch board 4 needs to be fixed, screws are screwed into the flange board and the outer wall of the preservation chamber 1.
The foregoing is merely a preferred embodiment of the utility model, and it should be noted that modifications could be made by those skilled in the art without departing from the principles of the utility model, which modifications would also be considered to be within the scope of the utility model.
Claims (7)
1. A cell cryopreservation device with high cell viability is characterized in that: comprises a preservation chamber (1), a material taking rod (2), a sample container (3), a switch plate (4), a first sealing plate (51), a second sealing plate (52), a third sealing plate (53) and a fourth sealing plate (54), wherein the preservation chamber (1) is internally provided with a freezing chamber (11) and a thawing chamber (13), a baffle rib (10) is fixed on the peripheral inner wall of the preservation chamber (1), an inner cavity opening of the thawing chamber (13) is formed by holes surrounded by the inner end surfaces of the baffle rib (10), the switch plate (4) is inserted and penetrates through the peripheral side wall of the preservation chamber (1), the inner cavity opening of the freezing chamber (11) is formed by holes surrounded by the inner end surfaces of the switch plate (4), the left side of the preservation chamber (1) penetrates into the thawing chamber (13) and stretches into the freezing chamber (11), the first sealing plate (51), the second sealing plate (52), the third sealing plate (53) and the fourth sealing plate (54) are sequentially fixed on the preservation chamber (1), the first sealing plate (51) is abutted against the left side end surface of the baffle rib (10) and seals the inner cavity opening of the thawing chamber (13) on the left side end surface of the baffle rib (13) and seals the material taking plate (4), the sample container (3) is fixed on the material taking rod (2) and is arranged in the freezing chamber (11).
2. The high cell viability cell cryopreservation apparatus of claim 1 wherein: after the first sealing plate (51) seals the thawing chamber (13) and the second sealing plate (52) seals the freezing chamber (11), a buffer chamber (12) is formed between the sealed thawing chamber (13) and the sealed freezing chamber (11).
3. The high cell viability cell cryopreservation apparatus of claim 1 wherein: the switch plate (4) is outwards drawn and the inner cavity opening of the freezing chamber is enlarged, the material taking rod (2) moves rightwards to enable the sample container (3) to be placed in the freezing chamber (13), the third sealing plate (53) is abutted against the right side end face of the blocking rib (10) and seals the inner cavity opening of the freezing chamber (13), and the fourth sealing plate (54) is abutted against the right side end face of the switch plate (4) and seals the inner cavity opening of the freezing chamber (11) after the expansion.
4. The high cell viability cell cryopreservation apparatus of claim 1 wherein: the preservation room (1) is provided with a feeding hole (61) and a material taking hole (62), and the feeding hole (61) and the material taking hole (62) are respectively communicated with the freezing room (11) and the thawing room (13) correspondingly.
5. The high cell viability cell cryopreservation apparatus of claim 1 wherein: the freezing chamber (11) is frozen by liquid nitrogen, and a liquid nitrogen access pipe is arranged on the outer wall of the preservation chamber (1).
6. The high cell viability cell cryopreservation apparatus of claim 1 wherein: the area of the fourth sealing plate (54) is larger than that of the first sealing plate (51), the area of the first sealing plate (51) is larger than that of the third sealing plate (53), and the area of the third sealing plate (53) is larger than that of the second sealing plate (52).
7. The high cell viability cell cryopreservation apparatus of claim 1 wherein: the switch board (4) is provided with a flange board which is fixedly connected with the outer wall surface of the preservation chamber (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322012717.5U CN220343384U (en) | 2023-07-28 | 2023-07-28 | Cell cryopreserving device with high cell viability |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322012717.5U CN220343384U (en) | 2023-07-28 | 2023-07-28 | Cell cryopreserving device with high cell viability |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220343384U true CN220343384U (en) | 2024-01-16 |
Family
ID=89481517
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322012717.5U Active CN220343384U (en) | 2023-07-28 | 2023-07-28 | Cell cryopreserving device with high cell viability |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220343384U (en) |
-
2023
- 2023-07-28 CN CN202322012717.5U patent/CN220343384U/en active Active
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