CN209965078U

CN209965078U - Cell cryopreservation device for biotechnology research

Info

Publication number: CN209965078U
Application number: CN201920751610.3U
Authority: CN
Inventors: 赖秀贤
Original assignee: Individual
Current assignee: Individual
Priority date: 2019-05-23
Filing date: 2019-05-23
Publication date: 2020-01-21
Anticipated expiration: 2029-05-23

Abstract

The utility model discloses a cell cryopreserving device for biotechnology research, including liquid nitrogen refrigeration low-temperature chamber and liquid nitrogen holding vessel, the both sides inner wall equidistance of liquid nitrogen refrigeration low-temperature chamber is equipped with the spout, and is in the inner wall sliding connection of two spouts of same height and puts the thing board, the outer wall of putting the thing board is opened there is the nitrogen passing hole, and the top outer wall welding of putting the thing board has the friction arch, and the thing case has been placed to the bellied top outer wall of friction, one side outer wall that liquid nitrogen refrigeration low-temperature chamber is close to the bottom is pegged graft and is had the denitrogenation pipe, and the outer wall of denitrogenation pipe is equipped with the denitrogenation valve, one side outer wall that liquid nitrogen refrigeration low. The utility model discloses a be provided with the measuring pump, improve the degree of accuracy that the liquid nitrogen advances the volume, guarantee the inside constancy of temperature of liquid nitrogen refrigeration cryotank, improve the frozen effect of cell, the setting up of time-recorder, denitrogenation pipe and denitrogenation valve makes the inside liquid nitrogen of liquid nitrogen refrigeration cryotank change regularly, guarantees the freezing effect of device.

Description

Cell cryopreservation device for biotechnology research

Technical Field

The utility model relates to a cell cryopreserving technical field especially relates to a cell cryopreserving device for biotechnology research.

Background

With the development of cytology, scientists in the field of biotechnology have increasingly studied cells, and for cells of interest, the cells need to be cryopreserved during a period of non-research, and a cell cryopreservation device for biotechnological research is needed during the cryopreservation of the cells.

The existing cell freezing device for biotechnological research generally performs cell freezing through a liquid nitrogen refrigeration cryotank, but the cell freezing device has certain disadvantages, when liquid nitrogen is added into the liquid nitrogen refrigeration cryotank, the liquid nitrogen amount in the liquid nitrogen refrigeration cryotank is generally monitored through a liquid level sensor, but the liquid nitrogen is formed by air pressurization, cooling and concentration, and the amount of the liquid nitrogen cannot be determined according to the volume, so that the error of the liquid nitrogen input amount can occur, and the freezing effect is reduced.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a cell freezing device for biotechnology research.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a cell cryopreserving device for biotechnology research, includes liquid nitrogen refrigeration low-temperature chamber and liquid nitrogen holding vessel, the both sides inner wall equidistance of liquid nitrogen refrigeration low-temperature chamber is equipped with the spout, and is in the inner wall sliding connection of two spouts of same height and puts the thing board, the outer wall of putting the thing board is opened there is the nitrogen hole, and puts the welding of the top outer wall of thing board and has the friction arch, and the thing case has been placed to the bellied top outer wall of friction, one side outer wall that liquid nitrogen refrigeration low-temperature chamber is close to the bottom is pegged graft and is had the denitrogen pipe, and the outer wall of denitrogen pipe is equipped with the denitrogen valve, one side outer wall that liquid nitrogen refrigeration low-temperature chamber is located denitrogen pipe top is equipped.

Further, the outer wall of the top of the storage box is provided with a box cover, the inner walls of two sides of the storage box are provided with upper fixing plates, and the inner walls of two sides of the storage box below the upper fixing plates are provided with lower fixing plates.

Furthermore, the same position of an upper fixing plate and a lower fixing plate is provided with a fixing hole, the inner wall of the fixing hole is welded with a projection, and the inner wall of the fixing hole is spliced with a cold storage pipe.

Furthermore, the outer wall of the bottom of the liquid nitrogen storage tank is provided with two support columns, and the outer wall of the top of the liquid nitrogen storage tank is provided with a pouring cover.

Furthermore, a metering pump is arranged on the outer wall of one side of the liquid nitrogen storage tank, and the infusion end of the metering pump is inserted into the inner wall of the nitrogen infusion pipe.

Furthermore, the outer wall of the liquid nitrogen refrigeration low-temperature tank is fixed with a tank door through a hinge, and sealing strips are pasted at the joint of the tank door and the liquid nitrogen refrigeration low-temperature tank.

Furthermore, the timer and the metering pump are both connected with a switch, and the switch is connected with a power supply.

The utility model has the advantages that:

1. through being provided with the measuring pump, improve the degree of accuracy that the liquid nitrogen advances the volume, guarantee the inside constancy of temperature of liquid nitrogen refrigeration low temperature groove, improve the frozen effect of cell, the setting up of time-recorder, nitrogen discharging pipe and nitrogen discharging valve makes the inside liquid nitrogen of liquid nitrogen refrigeration low temperature groove change regularly, guarantees the freezing effect of device.

2. Through being provided with the spout, be convenient for put the change of thing board, put the thing and open the nitrogen hole that crosses that has on the board and make things convenient for passing through of liquid nitrogen for everywhere temperature is comparatively even in the liquid nitrogen refrigeration low-temperature chamber, and the friction arch on putting the thing board improves the stability that the storage box was placed.

3. Through being provided with upper fixed plate and bottom plate, be convenient for carry on the cold fixed of depositing the pipe, the arch is hemispherical, reduces the frictional force that the cold outer wall of depositing the pipe received, reduces the wearing and tearing that the cold was deposited the pipe when will cold deposit the pipe fixed.

Drawings

FIG. 1 is a schematic structural diagram of a cell cryopreservation device for biotechnology research according to the present invention;

FIG. 2 is a schematic view of the internal structure of a storage box of a cell cryopreservation device for biotechnology research provided by the present invention;

fig. 3 is a schematic structural view of a liquid nitrogen refrigeration cryotank of a cell cryopreservation device for biotechnology research.

In the figure: 1-liquid nitrogen refrigeration low-temperature tank, 2-nitrogen discharge pipe, 3-nitrogen discharge valve, 4-timer, 5-chute, 6-friction bulge, 7-storage box, 8-upper fixed plate, 9-box cover, 10-lower fixed plate, 11-box cover, 12-nitrogen conveying pipe, 13-metering pump, 14-liquid nitrogen storage tank, 15-support column, 16-nitrogen passing hole, 17-storage plate, 18-cold storage pipe, 19-bulge, 20-tank door and 21-sealing strip.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Referring to fig. 1-3, a cell cryopreservation device for biotechnology research, including liquid nitrogen refrigeration low-temperature chamber 1 and liquid nitrogen holding vessel 14, the equidistance of the both sides inner wall of liquid nitrogen refrigeration low-temperature chamber 1 has spout 5 through the fix with screw, and be in the inner wall sliding connection of two spout 5 of co-altitude puts the thing board 17, the outer wall of putting the thing board 17 is opened has nitrogen passing hole 16, and the welding of the top outer wall of putting the thing board 17 has friction bulge 6, thing case 7 has been placed to the top outer wall of friction bulge 6, one side outer wall that liquid nitrogen refrigeration low-temperature chamber 1 is close to the bottom is pegged graft and is had nitrogen discharge pipe 2, and the outer wall of nitrogen discharge pipe 2 has nitrogen discharge valve 3 through the fix with screw, one side outer wall that liquid nitrogen refrigeration low-temperature chamber 1 is located nitrogen discharge pipe 2 top has timer 4 through the fix with screw, and the.

In the utility model, the outer wall of the top of the storage box 7 is provided with a box cover 9, the inner walls of two sides of the storage box 7 are fixed with an upper fixing plate 8 through screws, the inner walls of two sides of the storage box 7 below the upper fixing plate 8 are fixed with a lower fixing plate 10 through screws, the same positions of the upper fixing plate 8 and the lower fixing plate 10 are both provided with fixing holes, the inner wall of the fixing holes is welded with a bulge 19, the inner wall of the fixing holes is inserted with a cold storage pipe 18, the outer wall of the bottom of a liquid nitrogen storage tank 14 is fixed with two support columns 15 through screws, the outer wall of the top of the liquid nitrogen storage tank 14 is provided with a pouring cover 11, the outer wall of one side of the liquid nitrogen storage tank 14 is fixed with a metering pump 13 through screws, the transfusion end of the metering pump 13 is inserted in the inner wall of a nitrogen conveying pipe 12, the outer wall of the liquid nitrogen refrigeration low-temperature tank, the timer 4 and the metering pump 13 are both connected with a switch, and the switch is connected with a power supply.

The working principle is as follows: when the freezing device is used, the cold storage pipe 18 is placed on the inner wall of a fixing hole formed in the same position of the upper fixing plate 8 and the lower fixing plate 10, the friction force on the cold storage pipe 18 is reduced by the hemispherical protrusion 19 on the inner wall of the fixing hole, the cold storage pipe 18 is placed completely, the box cover 9 is covered, the slot door 20 of the liquid nitrogen refrigeration low-temperature slot 1 is opened, the storage box 7 is placed on the storage plate 17, the slot door 20 is closed, the sealing strip 21 is pasted at the joint of the slot door 20 and the liquid nitrogen refrigeration low-temperature slot 1, the metering pump 13 is started to convey liquid nitrogen to the inside of the liquid nitrogen refrigeration low-temperature slot 1, and after the conveying capacity of the liquid nitrogen reaches an index, the.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A cell freezing device for biotechnology research comprises a liquid nitrogen refrigeration cryotank (1) and a liquid nitrogen storage tank (14), it is characterized in that sliding chutes (5) are arranged on the inner walls of the two sides of the liquid nitrogen refrigeration low-temperature tank (1) at equal intervals, and the inner walls of the two sliding chutes (5) at the same height are connected with an object placing plate (17) in a sliding way, the outer wall of the object placing plate (17) is provided with a nitrogen passing hole (16), the outer wall of the top of the object placing plate (17) is welded with a friction bulge (6), the outer wall of the top of the friction bulge (6) is provided with an object placing box (7), a nitrogen discharge pipe (2) is inserted in the outer wall of one side of the liquid nitrogen refrigeration low-temperature tank (1) close to the bottom end, and the outer wall of the nitrogen discharging pipe (2) is provided with a nitrogen discharging valve (3), the outer wall of one side of the liquid nitrogen refrigeration low-temperature tank (1) above the nitrogen discharging pipe (2) is provided with a timer (4), and a nitrogen conveying pipe (12) is inserted into the outer wall of the top of the liquid nitrogen refrigeration low-temperature tank (1).

2. The cell cryopreservation device for biotechnological research according to claim 1 is characterized in that a box cover (9) is arranged on the outer wall of the top of the storage box (7), upper fixing plates (8) are arranged on the inner walls of two sides of the storage box (7), and lower fixing plates (10) are arranged on the inner walls of two sides of the storage box (7) below the upper fixing plates (8).

3. The cell cryopreservation device for biotechnological research according to claim 2 is characterized in that fixing holes are formed in the same positions of the upper fixing plate (8) and the lower fixing plate (10), a bulge (19) is welded to the inner wall of each fixing hole, and a cold storage tube (18) is inserted into the inner wall of each fixing hole.

4. The device for freezing and preserving cells for biotechnological research according to claim 1 is characterized in that the outer wall of the bottom of the liquid nitrogen storage tank (14) is provided with two support columns (15), and the outer wall of the top of the liquid nitrogen storage tank (14) is provided with a pouring cover (11).

5. The cell cryopreservation device for biotechnological research according to claim 1, characterized in that a metering pump (13) is arranged on the outer wall of one side of the liquid nitrogen storage tank (14), and the infusion end of the metering pump (13) is inserted into the inner wall of the nitrogen infusion tube (12).

6. The cell cryopreservation device for biotechnological research according to claim 1 is characterized in that a tank door (20) is fixed to the outer wall of the liquid nitrogen refrigeration cryotank (1) through a hinge, and sealing strips (21) are adhered to the joints of the tank door (20) and the liquid nitrogen refrigeration cryotank (1).

7. The device for freezing and preserving cells for biotechnological research according to claim 1 characterized in that the timer (4) and the metering pump (13) are both connected with a switch, and the switch is connected with a power supply.