CN215555811U

CN215555811U - Cell procedural cooling device for thin straws

Info

Publication number: CN215555811U
Application number: CN202121362654.0U
Authority: CN
Inventors: 刘佳
Original assignee: Shenzhen Zhongjia Biomedical Technology Co ltd
Current assignee: Shenzhen Zhongjia Biomedical Technology Co ltd
Priority date: 2021-06-18
Filing date: 2021-06-18
Publication date: 2022-01-18
Anticipated expiration: 2031-06-18

Abstract

The utility model discloses a cell procedural cooling device for a straw, which comprises an outer cooling box body, an inner cooling box body, a heat preservation layer and a box cover, wherein the inner cooling box body and the heat preservation layer are respectively arranged in an inner cavity of the outer cooling box body, the heat preservation layer is arranged between the inner wall of the outer cooling box body and the outer wall of the inner cooling box body, an injection pipe for injecting isopropanol and a plurality of pipe cavities for storing the straw are arranged on the inner cooling box body, and the box cover is hermetically covered on the outer cooling box body and the injection pipe. The programmed cell cooling device for the straw is simple and convenient to operate, large in sample processing amount and low in cost.

Description

Cell procedural cooling device for thin straws

Technical Field

The utility model relates to the technical field of cell storage and cold storage, in particular to a cell procedural cooling device for a thin straw.

Background

At present, two methods for freezing and storing programmed cooling of the thin wheat tube cells are available: the first method is that the cells and the freezing medium are injected into a thin straw, the thin straw is placed in an environment of 4 ℃ for 30min, then placed in an environment of-20 ℃ for 1h, and then placed in an environment of-80 ℃ for overnight, and then transferred with liquid nitrogen for long-term storage, the method is complex to operate, wastes time and labor, the freezing effect is influenced by multiple factors, and the freezing effect cannot be controlled, so that the long-term storage is not facilitated; the second method is to inject the cells and the frozen stock solution into a thin straw, then put the thin straw into a programmed cooling instrument to realize gradient cooling, and then put the thin straw into liquid nitrogen for long-term storage. However, the program cooling instrument is expensive, the consumption of liquid nitrogen is large, the operation is not simple and convenient, and if the sample processing capacity is not large, the cost is high.

The above background disclosure is only for the purpose of assisting understanding of the concept and technical solution of the present invention and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problems, the utility model provides a cell procedural cooling device for a straw, which is simple and convenient to operate, large in sample processing amount and low in cost.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the utility model discloses a cell procedural cooling device for a straw, which comprises an outer cooling box body, an inner cooling box body, a heat preservation layer and a box cover, wherein the inner cooling box body and the heat preservation layer are respectively arranged in an inner cavity of the outer cooling box body, the heat preservation layer is arranged between the inner wall of the outer cooling box body and the outer wall of the inner cooling box body, an injection pipe for injecting isopropanol and a plurality of pipe cavities for storing the straw are arranged on the inner cooling box body, and the box cover is hermetically covered on the outer cooling box body and the injection pipe.

Preferably, the upper port of the inner cooling box body is connected with the upper port of the outer cooling box body in a clamping manner.

Preferably, the lower surface department of the last port of box body is equipped with first block portion in the cooling, the upper surface department of the last port of the outer box body of cooling is equipped with second block portion, first block portion with second block portion mutually supports so that box body can fix a position fixed setting in the cooling on the outer box body of cooling.

Preferably, a platform part is arranged on the cooling inner box body, the upper surface of the platform part is recessed at the upper port of the cooling inner box body, a plurality of first through holes are arranged on the platform part, and the injection pipe is formed by upward extending one of the first through holes of the platform part so that the upper port of the injection pipe is communicated with the inner cavity of the cooling outer box body; a plurality of the tube cavity is from a plurality of the first through holes of the platform part are formed by extending downwards, and the bottom and the side wall of the tube cavity are sealing structures.

Preferably, the lower surface of the box cover is provided with a first flange, and the position of the first flange corresponds to the position of the injection pipe, so that the first flange is sealed and covered at the upper port of the injection pipe when the box cover is sealed and covered at the upper port of the outer cooling box body.

Preferably, be equipped with temperature sensor on the internal surface of lid, be equipped with temperature display element on the surface of lid, temperature display element connects temperature sensor is in order to be used for showing the temperature that temperature sensor sensed.

Preferably, the bottom of the cooling outer box body is further provided with an outlet, and a sealing cover is arranged at a port of the outlet.

Preferably, the edge of the bottom of the outer box body of cooling is provided with a second flange, the lower surface of the bottom of the outer box body of cooling is provided with a second through hole and a third flange which form the discharge port, the third flange is arranged from the lower surface of the second through hole in a downward extending manner, the sealing cover is connected at the lower port of the third flange in a sealing manner, and the lower surface of the sealing cover is recessed in the lower surface of the second flange.

Preferably, the heat insulation layer is formed by medical absorbent cotton.

Preferably, the outer cooling box body is made of polycarbonate material, and the inner cooling box body and the box cover are made of polyethylene material respectively.

Compared with the prior art, the utility model has the beneficial effects that: according to the programmed cell cooling device for the wheat straws, provided by the utility model, the plurality of wheat straws can be respectively stored in the tube cavity of the cooling inner box body, isopropanol can be injected into the inner cavity of the cooling outer box body through the injection tube of the cooling inner box body so as to further store the wheat straws in the cavity environment containing the isopropanol, and the programmed cell cooling device for the wheat straws is directly placed in the environment at minus 80 ℃ so as to realize the programmed freezing and cooling of the wheat straw cells, so that the programmed cell cooling device is simple and convenient to operate, large in sample processing amount and low in cost.

In a further scheme, the box body is fixedly connected to the outer cooling box body in the cooling process, and the heat preservation layer is arranged between the box body and the outer cooling box body in the cooling process and is not easy to lose.

In a further scheme, isopropanol is stored in the cavity formed by the platform part of the box body in the cooling process and the inner wall of the outer box body in the cooling process, and the upper port of the injection pipe for injecting the isopropanol protrudes out of the platform part of the box body in the cooling process, so that the isopropanol is not easy to leak. Furthermore, the lower surface of the box cover is provided with a first flange, so that the upper port of the injection pipe is covered with the first flange in a sealing way at the upper port of the cooling outer box body, and the possibility of leakage of isopropanol is further avoided.

In a further scheme, the box cover is provided with a temperature sensor and a temperature display unit, so that the temperature in the cell procedural cooling device of the straw can be displayed in real time. Furthermore, the bottom of the cooling outer box body is provided with an outlet, so that the isopropanol injected into the inner cavity of the cooling outer box body can be discharged from the outlet, and the operation is convenient.

Drawings

FIG. 1 is a schematic view of the overall structure of a device for cooling down the cell program of a straw according to a preferred embodiment of the present invention;

FIG. 2 is a schematic structural view of the cell procedural cooling device of the straw of FIG. 1 when a box cover is opened;

FIG. 3 is one of the schematic exploded structural views of the device for cooling down cell progressions of the straw of FIG. 1;

fig. 4 is a second schematic diagram of the explosion structure of the device for cooling the cell program of the straw of fig. 1.

Detailed Description

In order to make the technical problems, technical solutions and advantageous effects to be solved by the embodiments of the present invention more clearly apparent, the present invention is further described in detail below with reference to the accompanying drawings and the embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the utility model and are not intended to limit the utility model.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element. In addition, the connection may be for either a fixing function or a circuit connection function.

It is to be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship indicated in the drawings for convenience in describing the embodiments of the present invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed in a particular orientation, and be in any way limiting of the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of the present invention, "a plurality" means two or more unless specifically limited otherwise.

The straw is a slender tube with two sealed ends, cells are filled in the straw, and low-temperature preservation is usually needed, but the conventional method for cooling the straw by using the cell cold preservation method is complex in operation, small in sample treatment amount and high in cost, so that the utility model provides the device for cooling the straw by using the cell program, which is simple and convenient to operate, large in sample treatment amount and low in cost.

As shown in fig. 1 to 4, the device for cooling down the cell programed by the straw according to the preferred embodiment of the present invention includes an outer cooling box 10, an inner cooling box 20, a heat insulating layer 30 and a box cover 40, wherein the inner cooling box 20 and the heat insulating layer 30 are respectively disposed in an inner cavity 15 of the outer cooling box 10, the heat insulating layer 30 is disposed between an inner wall of the outer cooling box 10 and an outer wall of the inner cooling box 20, the inner cooling box 20 is provided with an injection tube 21 for injecting isopropyl alcohol and a plurality of tube cavities 22 for storing the straw, and the box cover 40 is hermetically covered on the outer cooling box 10 and the injection tube 21; wherein the isopropyl alcohol injected from the injection pipe 21 is stored between the inner wall of the cooling outer case 10 and the outer wall of the cooling inner case 20, that is, the heat insulating layer 30 is soaked in the isopropyl alcohol solution. In this embodiment, the outer cooling box 10, the inner cooling box 20, the insulating layer 30 and the box cover 40 are all cylindrical.

The upper port 23 of the inner cooling box body 20 is connected with the upper port of the outer cooling box body 10 in a clamping manner; specifically, a first engaging portion 231 is disposed at a lower surface of the upper port 23 of the inner cooling box 20, a second engaging portion 111 is disposed at an upper surface of the upper port 11 of the outer cooling box 10, and the first engaging portion 231 and the second engaging portion 111 are mutually engaged to fix the inner cooling box 20 on the outer cooling box 10. That is, in the present embodiment, the cooling inner case 20 and the cooling outer case 10 are connected in a sealing manner and are not detachable (e.g. they can be disposed in an interference fit manner), wherein the insulating layer 30 is disposed therebetween to avoid loss.

The cooling inner box body 20 is provided with a platform part 24, the upper surface of the platform part 24 is recessed at the upper port 23 of the cooling inner box body 20, the platform part 24 is provided with a plurality of first through holes 241, and the injection pipe 21 is formed by extending upwards from one of the first through holes 241 of the platform part 24 so that the upper port of the injection pipe 21 is communicated with the inner cavity 15 of the cooling outer box body 10; the plurality of tube cavities 22 are formed by extending downwards from the plurality of first through holes 241 of the platform portion 24, and the bottom and the side walls of the tube cavities 22 are sealing structures so that the interior of the tube cavities 22 is not communicated with the inner cavity 15 of the cooling outer box 10. Wherein injection pipe 21 protrusion sets up on platform portion 24, can not appear the condition that isopropyl alcohol leaked when placing at ordinary times. In this embodiment, 61 first through holes 241 are arranged on the platform part 24, wherein 60 first through holes 241 are respectively connected to 60 tube cavities 22, so that the device for cooling the cell program of the straw can cold store 60 straws at one time, thereby improving the efficiency. The first through hole 241 is circular, and the injection pipe 21 and the pipe cavity 22 are both cylindrical structures.

The lower surface of the box cover 40 is provided with a first flange 41, the position of the first flange 41 corresponds to the position of the injection pipe 21, so that the first flange 41 is sealed and covered on the upper port of the injection pipe 21 when the box cover 40 is sealed and covered on the upper port 11 of the cooling outer box body 10; specifically, an internal thread may be provided on the first flange 41, an external thread may be provided at the upper port of the injection pipe 21, and the injection pipe 21 and the first flange 41 are connected in a sealing manner by the matching of the external thread and the internal thread. A temperature sensor (not shown) is disposed on the inner surface of the box cover 40, a temperature display unit 42 is disposed on the outer surface of the box cover 40, and the temperature display unit 42 is connected to the temperature sensor for displaying the temperature sensed by the temperature sensor.

The bottom of the cooling outer box 10 is also provided with an outlet 12, and a sealing cover 13 is arranged at the port of the outlet 12. Specifically, the edge of the bottom of the cooling outer box 10 is provided with a second flange 14, the lower surface of the bottom of the cooling outer box 10 is provided with a second through hole 121 and a third flange 122 which form the discharge port 12, the third flange 122 extends downward from the lower surface of the second through hole 121, the sealing cover 13 is hermetically connected at the lower port of the third flange 122, and the lower surface of the sealing cover 13 is recessed in the lower surface of the second flange 14, so that the whole cell programmed cooling device of the straw is supported by the lower surface of the second flange 14. By providing the discharge port 12, it is not necessary to pour out isopropyl alcohol from the injection pipe 21, and the operation is more convenient.

The heat-insulating layer 30 is made of medical absorbent cotton, the outer cooling box body 10 is made of polycarbonate material and has the characteristics of acid resistance, oil resistance, strong impact resistance and the like, and the inner cooling box body 20 and the box cover 40 are made of polyethylene material respectively.

When the cooling outer box body 10 is used, isopropanol is injected into the inner cavity 15 of the cooling outer box body 10 through the injection pipe 21, the thin straw needing to be cooled and stored is placed into the pipe cavity 22, the box cover 40 is covered, and then the cell programmed cooling device of the thin straw is placed into a refrigerator with the temperature of 80 ℃ below zero for cooling and storage. At present, the temperature of the straw can be reduced only by using a program cooling instrument, and the program cooling instrument is expensive, high in cost, power-consuming and time-consuming in liquid nitrogen; the preferred embodiment of the utility model solves the problems that in the prior art, the program cooling instrument can only be used for freezing and storing the straw cells, the cost is higher, and the cells can not be cooled and frozen and stored by the program after the instrument fails in use, so that the storage quality is influenced.

The foregoing is a more detailed description of the utility model in connection with specific preferred embodiments and it is not intended that the utility model be limited to these specific details. For those skilled in the art to which the utility model pertains, several equivalent substitutions or obvious modifications can be made without departing from the spirit of the utility model, and all the properties or uses are considered to be within the scope of the utility model.

Claims

1. The utility model provides a cell procedural heat sink of wheat straw, its characterized in that, box body, heat preservation and lid in the outer box body of cooling, the box body with the heat preservation sets up respectively in the inner chamber of the outer box body of cooling, wherein the heat preservation sets up the inner wall of the outer box body of cooling with between the outer wall of box body in the cooling, be equipped with the filling tube and a plurality of being used for depositing that are used for injecting into isopropyl alcohol on the box body in the cooling the tube cavity body of wheat straw, the sealed lid of lid closes the outer box body of cooling with on the filling tube.

2. The device for cooling down cells in programmed manner of straw according to claim 1, wherein the upper port of the inner cooling box body is connected with the upper port of the outer cooling box body in a snap-fit manner.

3. The device for cooling the tubule during the cell programming according to claim 2, wherein a first engaging portion is disposed on the lower surface of the upper port of the inner cooling box, a second engaging portion is disposed on the upper surface of the upper port of the outer cooling box, and the first engaging portion and the second engaging portion are engaged with each other to fix the inner cooling box on the outer cooling box.

4. The device for cooling the straws in the procedure of cell programming according to claim 2, wherein the inner cooling box body is provided with a platform part, the upper surface of the platform part is recessed at the upper port of the inner cooling box body, the platform part is provided with a plurality of first through holes, and the injection tube is formed by extending upwards from one of the first through holes of the platform part so that the upper port of the injection tube is communicated with the inner cavity of the outer cooling box body; a plurality of the tube cavity is from a plurality of the first through holes of the platform part are formed by extending downwards, and the bottom and the side wall of the tube cavity are sealing structures.

5. The device for cooling down the cell program of the straw as claimed in claim 1, wherein the lower surface of the box cover is provided with a first flange corresponding to the position of the filling tube, so that the first flange is sealed and covered at the upper port of the filling tube when the box cover is sealed and covered at the upper port of the outer cooling box body.

6. The device for cooling down cells during procedure of straws as claimed in claim 1, wherein a temperature sensor is arranged on the inner surface of the box cover, a temperature display unit is arranged on the outer surface of the box cover, and the temperature display unit is connected with the temperature sensor for displaying the temperature sensed by the temperature sensor.

7. The device for cooling down cells in the programmed state of straws as claimed in claim 1, wherein the bottom of the outer cooling box is further provided with an outlet, and a sealing cover is provided at the port of the outlet.

8. The device for cooling down cells during procedure of straw formation as claimed in claim 7, wherein a second flange is provided at an edge of a bottom of the outer cooling box, a second through hole and a third flange constituting the outlet are provided at a lower surface of the bottom of the outer cooling box, the third flange extends downward from a lower surface of the second through hole, the sealing cover is sealingly connected at a lower port of the third flange, and a lower surface of the sealing cover is recessed in a lower surface of the second flange.

9. The device for cooling down cells during the process of growing the cells in the straw according to any one of claims 1 to 8, wherein the heat-insulating layer is formed by medical absorbent cotton.

10. The device for cooling down cells during procedure of straws as claimed in any one of claims 1 to 8, wherein the outer cooling box body is made of polycarbonate material, and the inner cooling box body and the box cover are made of polyethylene material.