CN210642163U

CN210642163U - Program cooling box for freezing storage bag

Info

Publication number: CN210642163U
Application number: CN201921469857.2U
Authority: CN
Inventors: 杨玮娴; 王肇光; 刘欢; 高大鹏
Original assignee: Jiangxi Yinfeng Dingcheng Bio Engineering Co ltd; Yinfeng Biological Group Ltd
Current assignee: Jiangxi Yinfeng Dingcheng Bio Engineering Co ltd; Yinfeng Biological Group Ltd
Priority date: 2019-09-05
Filing date: 2019-09-05
Publication date: 2020-06-02
Anticipated expiration: 2029-09-05

Abstract

The utility model discloses a program cooling box for freezing bags, which comprises a cooling box body; the cooling box body be equipped with the box main part, box main part top is equipped with the opening, box main part four sides are equipped with the lateral wall, box main part bottom is equipped with the box bottom, the lateral wall on four sides and box bottom enclose into one and put the thing cavity, the opening part at box main part top is equipped with the lid, has placed the cell body structure in putting the thing cavity, the cell body structure be the sample groove, can place the cryopreserving bag in the sample groove, the below in sample groove, put and be equipped with isopropanol liquid in the thing cavity, the sample groove be equipped with and take out the structure, the sample groove be equipped with insulation construction. The isopropanol solution can enable the program cooling box to be placed at-80 ℃, so that the sample in the box is slowly cooled at the speed of-1 ℃/min, and the damage to cells caused by too fast cooling is reduced. The utility model provides a programming cooling box is to the design of bulky cell cryopreserving bag, has filled the blank of bulky cell sample programming cooling box in the market.

Description

Program cooling box for freezing storage bag

Technical Field

The utility model relates to a biological medicine field specifically is a procedure cooling box suitable for freezing bag cryopreserved, constitute including box main part, lid and sample groove triplex. The utility model provides a procedure cooling box is applicable to the procedure cooling process use of big volume cell cryopreserving bag.

Background

The cells need to be stored in liquid nitrogen at-196 ℃ for a long time, and the temperature of the cells needs to be slowly reduced in the process of cell cryopreservation in order to avoid the damage of ice crystals to the cells in the process of cryopreservation. At present, a program cooling instrument or a program cooling box is generally used in the program cooling process, and the program cooling instrument is expensive and is not suitable for small-scale production, preparation and use, so most of the program cooling boxes are adopted.

At present, the known procedural cooling boxes are designed by 2ml freezing tubes, the freezing samples are small in size, large-size cell samples need to be subpackaged in a large quantity, the workload is large, the time consumption is long, the quantity demand on the procedural cooling boxes is large, and the cost control is not facilitated.

Furthermore, since program thermometers are expensive, a laboratory is generally equipped with only one program thermometer, and there is no alternative program thermometer when the equipment is out of order or maintained.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is that the programming cooling box known at present is that 2ml freezes deposits the pipe design, freezes to deposit the sample volume less, needs a large amount of partial shipment to bulky cell sample, and work load is big, and is consuming time long, and great to the quantity demand of programming cooling box, is unfavorable for cost control. Furthermore, since program thermometers are expensive, a laboratory is generally equipped with only one program thermometer, and there is no alternative program thermometer when the equipment is out of order or maintained.

In order to solve the above technical problem, the utility model discloses a following technical means:

a program cooling box for a freezing storage bag comprises a cooling box body; the cooling box body be equipped with the box main part, box main part top is equipped with the opening, box main part four sides are equipped with the lateral wall, box main part bottom is equipped with the box bottom, the lateral wall on four sides and box bottom enclose into one and put the thing cavity, the opening part at box main part top is equipped with the lid, has placed the cell body structure in putting the thing cavity, the cell body structure be the sample groove, can place the cryopreserving bag in the sample groove, the below in sample groove, put and be equipped with isopropanol liquid in the thing cavity, the sample groove be equipped with and take out the structure, the sample groove be equipped with insulation construction. The isopropanol solution can enable the program cooling box to be placed at-80 ℃, so that the sample in the box is slowly cooled at the speed of-1 ℃/min, and the damage to cells caused by too fast cooling is reduced.

Preferably, the present invention further provides:

the size of the box cover is matched with the size of the opening at the top of the box body, the box cover can enable the storage cavity to form sealing, and a sealing structure is arranged at the opening joint of the box cover and the top of the box body.

The taking-out structure is the bellying that sets up in sample groove both sides, the quantity of bellying is two, is located both ends about the sample groove respectively, the bellying of bellying is higher than the sample groove and places the level in putting the thing cavity. The sample groove is used for being taken out and separated from the box main body, and the sample groove can be detached and is convenient for filling isopropanol.

The heat preservation structure is characterized in that a sponge layer is arranged on the outer side of the sample groove.

The width of the interior of the sample groove is larger than the thickness of the cryopreservation bag.

The number of the sample grooves is multiple, the sample grooves are arranged in the storage cavity of the cooling box body, the lower ends of the sample grooves are immersed in the isopropanol, and the sample grooves can be detached to facilitate the injection of the isopropanol.

The box body, the box cover and the sample groove are all made of low-temperature resistant materials.

The sealing structure is a sealing ring which is divided into an upper layer and a lower layer and is arranged at the opening joint of the box cover and the top of the box main body.

The utility model provides a programming cooling box is to the design of bulky cell cryopreserving bag, has filled the blank of bulky cell sample programming cooling box in the market.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention.

Fig. 2 is a sectional view of the temperature programmed box of the utility model.

Fig. 3 is a schematic structural diagram of a sample tank in the program cooling box of the utility model.

Fig. 4 is a schematic structural diagram of the sealing structure of the present invention.

Description of reference numerals: 1-a cartridge body; 2-a box cover; 3-a tank structure; 301-sample trenches; 4-freezing and storing the bags; 5-a boss; 6-upper sealing ring; 7-lower sealing ring.

Detailed Description

The present invention will be further described with reference to the following examples.

Specific example 1:

referring to fig. 1, 2 and 3, the utility model relates to a program cooling box for freezing storage bag 4, the cooling box body is provided with a box main body 1, the top of the box main body 1 is provided with an opening, the four sides of the box main body 1 are provided with side walls, the bottom of the box main body 1 is provided with a box bottom, the four sides of the side walls and the box bottom enclose a storage cavity, the opening at the top of the box main body 1 is provided with a box cover 2, a cell body structure 3 is arranged in the storage cavity, the cell body structure 3 is a sample groove 301, the freezing storage bag 4 can be placed in the sample groove 301, isopropanol liquid is arranged below the sample groove 301 and in the storage cavity, the sample groove 301 is provided with a taking-out structure, the sample groove 301 is provided with a heat insulation structure, the size of the box cover 2 is matched with the opening size at the top of the box main body 1, the box cover 2 can seal the storage cavity, a sealing structure is arranged at, the bulge 5 of taking out the structure for setting up in sample groove 301 both sides, the quantity of bulge 5 is two, be located sample groove 301 respectively about both ends, the bulge of bulge 5 is higher than sample groove 301 and places the level in putting the thing cavity, insulation system is equipped with the sponge layer for the sample groove 301 outside, the inside width of cell body of sample groove 301 is greater than the thickness of freezing bag 4, sample groove 301 quantity is many, many sample grooves 301 set up in the thing cavity of putting of cooling box body, the lower extreme submergence of sample groove 301 in isopropanol, sample groove 301 can dismantle and conveniently annotate isopropanol, box main part 1, lid 2 and sample groove 301 all adopt anti low temperature material.

Specific example 2:

referring to fig. 4, the utility model relates to a procedure cooling box for freezing bag 4 uses, seal structure are the sealing washer, and the sealing washer is two-layer about, is the opening junction department that upper sealing washer 6 and lower floor's sealing washer 7 set up at lid 2 and box main part 1 top respectively.

Specific example 3:

referring to fig. 1, 2 and 3, the utility model provides a program cooling box for freezing storage of a freezing storage bag, which comprises a box main body 1, a box cover 2 and a groove body structure 3; the box cover 2 is made of polyethylene PE material, the box cover 2 is arranged above the programmed cooling box main body 1, and the box cover 2 and the box main body 1 form a sealed whole; the box main body 1 is made of polycarbonate material, a groove body structure 3 is arranged in the box main body 1, and isopropanol is filled between the box main body 1 and the groove body structure 3; the tank body knot 3 is provided with 10 sample tanks 301 for placing the cryopreservation bags, and the lower parts of the sample tanks 301 extend into isopropanol; the freezing bag 4 is inserted into the sample groove 301; the bulge part 5 is positioned on the groove body structure 3 and used for taking out the groove body structure 3 and adding isopropanol into the groove body structure; the isopropanol can slowly reduce the temperature in the program cooling box, and avoid the damage to cells caused by too fast cooling.

Specific example 4:

the utility model provides a procedure cooling box operation description as follows:

firstly, taking out the groove body structure 3, adding isopropanol into the box main body 1, and then putting the groove body structure 3 into the box main body 1; putting the freezing bag into the corresponding sample groove 301; the box cover 2 is covered, and the whole programmed cooling box is put into an ultra-low temperature refrigerator for overnight at minus 80 ℃; and (4) taking out the cryopreservation bag after the temperature reduction is finished, transferring the cryopreservation bag into a cryopreservation box, and transferring the cryopreservation bag into liquid nitrogen at the temperature of 196 ℃ below zero for long-term storage.

Since the above description is only a specific embodiment of the present invention, but the protection of the present invention is not limited thereto, any person skilled in the art can think of equivalent changes or substitutions of the technical features of the present invention, and all are covered in the protection scope of the present invention.

Claims

1. A program cooling box for a freezing storage bag comprises a cooling box body; the method is characterized in that: the cooling box body be equipped with the box main part, box main part top is equipped with the opening, box main part four sides are equipped with the lateral wall, box main part bottom is equipped with the box bottom, the lateral wall on four sides and box bottom enclose into one and put the thing cavity, the opening part at box main part top is equipped with the lid, has placed the cell body structure in putting the thing cavity, the cell body structure be the sample groove, can place the cryopreserving bag in the sample groove, the below in sample groove, put and be equipped with isopropanol liquid in the thing cavity, the sample groove be equipped with and take out the structure, the sample groove be equipped with insulation construction.

2. The programmable cooling cartridge for use with a cryopreservation bag of claim 1, further comprising: the size of the box cover is matched with the size of the opening at the top of the box body, the box cover can enable the storage cavity to form sealing, and a sealing structure is arranged at the opening joint of the box cover and the top of the box body.

3. The programmable cooling cartridge for use with a cryopreservation bag of claim 1, further comprising: the taking-out structure is the bellying that sets up in sample groove both sides, the quantity of bellying is two, is located both ends about the sample groove respectively, the bellying of bellying is higher than the sample groove and places the level in putting the thing cavity.

4. The programmable cooling cartridge for use with a cryopreservation bag of claim 1, further comprising: the heat preservation structure is characterized in that a sponge layer is arranged on the outer side of the sample groove.

5. The programmable cooling cartridge for use with a cryopreservation bag of claim 1, further comprising: the width of the interior of the sample groove is larger than the thickness of the cryopreservation bag.

6. The programmable cooling cartridge for use with a cryopreservation bag of claim 1, further comprising: the number of the sample grooves is multiple, the sample grooves are arranged in the storage cavity of the cooling box body, the lower ends of the sample grooves are immersed in the isopropanol, and the sample grooves can be detached to facilitate the injection of the isopropanol.

7. The programmable cooling cartridge for use with a cryopreservation bag of claim 1, further comprising: the box body, the box cover and the sample groove are all made of low-temperature resistant materials.

8. A programmed cooling cartridge for use with a cryopreservation bag as claimed in claim 2, wherein: the sealing structure is a sealing ring which is divided into an upper layer and a lower layer and is arranged at the opening joint of the box cover and the top of the box main body.