CN212373916U

CN212373916U - Multifunctional program cooling box

Info

Publication number: CN212373916U
Application number: CN202021004349.XU
Authority: CN
Inventors: 刘梦梅; 刘慧莹
Original assignee: Wuhan Saiyunbo Biological Technology Co ltd
Current assignee: Wuhan Saiyunbo Biological Technology Co ltd
Priority date: 2020-06-04
Filing date: 2020-06-04
Publication date: 2021-01-19
Anticipated expiration: 2030-06-04

Abstract

The utility model relates to the technical field of biological experiment equipment, and discloses a multifunctional program cooling box, which comprises a cooling box shell, a cooling box inner container and a freezing storage pipe frame from the outside to the inside; the cooling box shell and the cooling box inner container form a closed cavity, and a refrigerant is filled in the cavity; the cooling box shell and the cooling box inner container are sealed through an annular buckle body, and the annular buckle body is hermetically connected to openings at the top ends of the cooling box shell and the cooling box inner container; the cryopreservation pipe frame is placed on the inner container of the cooling box; the box cover is connected with the cooling box shell in a clamping mode through the annular buckle body. The multifunctional program cooling box forms a closed cavity by matching the cooling box shell, the cooling box inner container and the annular buckle body, and the cavity is filled with a refrigerant, so that isopropanol can be prevented from leaking to cause safety accidents; in addition, the injection port and the plug block which are communicated with the cavity can be added and replaced after the isopropanol is used for a long time, so that the operation is more flexible and convenient.

Description

Multifunctional program cooling box

Technical Field

The utility model relates to a biological assay equipment technical field specifically is a multi-functional procedure cooling box.

Background

Since its inception, cell culture technology has become one of indispensable research methods in the field of natural science for a century now, and today, the basic technology of cryopreservation of cells is gaining increasing attention in the field of wide application of cell culture technology in the field of scientific research.

At present, there are two main methods for cell cryopreservation: non-vitrified and vitrified cryopreservation. The non-vitrification cryopreservation is to utilize refrigerators with various temperature grades to reduce the temperature to-7 ℃ to-80 ℃ in stages, and then directly put into liquid nitrogen for preservation, wherein the frozen cell suspension by the method has more or less ice crystals; the vitrification cryopreservation is a method for protecting suspended cells by using vitrification cryopreservation liquid formed by combining a plurality of high-concentration cryoprotectants, and directly putting liquid nitrogen into the vitrification cryopreservation liquid for cryopreservation, wherein the cell suspension frozen by the method does not have the formation of ice crystals, does not damage cell membranes and organelles, does not expose cells in high-concentration solutes for a long time to be damaged, and is the most ideal cryopreservation method; however, since the cryopreserved tube needs to be taken out of liquid nitrogen at-196 ℃ and immediately put into warm water at 37 ℃ during recovery, the temperature difference is large, and the glass ampoule is easy to explode and cause danger.

At present, the non-vitrified cryopreservation cells are mainly used for a program cooling box, the effect of reducing the temperature by 1 ℃ per minute is achieved mainly by adding 100% of isopropanol into the cryopreservation box, but the isopropanol has the defects of toxicity, volatility, flammability, easiness in absorbing moisture and the like, and most of isopropanol storage tanks of the cooling boxes in the existing market are cavities formed by box outer bodies and internal cryopreservation pipe frames, so that the isopropanol needs to be added periodically and repeatedly to ensure the cooling process. The frozen storage box is placed at minus 80 ℃ for 24 hours, so that the time and labor are wasted when the sample is transferred, and the isopropanol leaks out due to uneven strength, thereby causing safety accidents; in addition, although the freezing tube is vertical, in the process of transferring the cells to be frozen to the freezing tube, the freezing tube is not easy to stand, which brings inconvenience to the process, so that a multifunctional program cooling box is provided to solve the problems.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The utility model provides a be not enough to prior art, the utility model provides a multi-functional procedure cooling box possesses and prevents that isopropanol from revealing, freezing to deposit the pipe and shift in batches and be convenient for place and freeze advantages such as deposit the pipe, has solved on the current market most cooling box isopropanol hold up tank and be the cavity that the box external body and inside frozen pipe support formed, causes to need regularly to add isopropanol repeatedly and will wait to freeze the in-process that deposits the cell transfer to freezing and deposit the pipe, freezes and deposits the difficult problem of standing and shifting of pipe.

(II) technical scheme

For realize above-mentioned prevent that isopropanol from revealing, freezing the purpose that deposits the pipe and shift and be convenient for place the freezing pipe in batches, the utility model provides a following technical scheme: a multifunctional program cooling box comprises a cooling box shell, a cooling box inner container and a freezing storage pipe frame from outside to inside;

the cooling box shell and the cooling box inner container form a closed cavity, and a refrigerant is filled in the cavity; the cooling box shell and the cooling box inner container are sealed through an annular buckle body, and the annular buckle body is hermetically connected to openings at the top ends of the cooling box shell and the cooling box inner container;

the cryopreservation pipe frame is placed on the inner container of the cooling box;

the box cover is connected with the cooling box shell in a clamping mode through the annular buckle body.

Preferably, the cryopreservation pipe frame comprises a placing plate and a plurality of supporting columns, and the placing plate is provided with at least twenty-eight placing holes; the support columns are fixedly connected to the lower surface of the placing plate.

Preferably, the cooling box inner container is provided with a groove matched with the support column, and the groove is used for placing the cryopreservation pipe frame.

Preferably, be equipped with in the cooling box inner bag and be used for placing 2 mL's the first cylindricality cavity of freezing the pipe and be used for placing 5 mL's the second cylindricality cavity of freezing the pipe, the position one-to-one of first cylindricality cavity and second cylindricality cavity and the position of placing the hole, sixteen is no less than to the quantity of first cylindricality cavity.

Preferably, an injection port communicated with the cavity is formed in the cooling box shell, and a plug block is matched in the injection port.

(III) advantageous effects

Compared with the prior art, the utility model provides a multi-functional procedure cooling box possesses following beneficial effect:

1. the multifunctional program cooling box forms a closed cavity by matching the cooling box shell, the cooling box inner container and the annular buckle body, and the cavity is filled with a refrigerant, so that isopropanol can be prevented from leaking to cause safety accidents; in addition, the injection port and the plug block which are communicated with the cavity can be added and replaced after the isopropanol is used for a long time, so that the operation is more flexible and convenient.

2. This multi-functional procedure cooling box, through the use of freezing the pipe support of depositing, freeze and deposit and seted up a plurality of recess looks adaptations on the pillar that sets up on the pipe support and the cooling box inner bag, can make the separation of freezing pipe support and cooling box inner bag, freeze and deposit the pipe support and can use outside alone, solved and frozen the pipe and place the problem that is difficult for standing outward to the hole of placing that sets up is corresponding with first cylindricality cavity and second cylindricality cavity, practiced thrift and will freeze the time of depositing the pipe and shift to freezing the box in batches, made things convenient for the use.

Drawings

Fig. 1 is a schematic view of the overall structure of the present invention;

fig. 2 is a schematic sectional structure of the present invention;

FIG. 3 is an enlarged view of the point A in FIG. 2;

fig. 4 is a schematic structural view of the cryopreservation tube rack in the present application.

In the figure: the box comprises a box cover 1, a pull ring 101, a clamping groove 102, a cooling box shell 2, an injection opening 201, a plug 202, a cooling box inner container 3, a first cylindrical cavity 301, a second cylindrical cavity 302, a groove 303, a cavity 4, a freezing storage pipe frame 5, a placing plate 501, a supporting column 502, a placing hole 503 and an annular buckle 6.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, a multifunctional programmed cooling box comprises, from outside to inside, a cooling box housing 2, a cooling box inner container 3 and a freezing storage pipe frame 5, all of which are made of polyethylene PE material;

the cooling box shell 2 is a rectangular shell which is hollow inside and has a missing upper surface, the length is 18cm, the width is 13cm, the height is 12cm, annular buckle bodies 6 are arranged on the cooling box shell 2 and the cooling box inner container 3 and are used for hermetically connecting the cooling box shell 2 and the cooling box inner container 3, so that the cooling box shell 2 and the cooling box inner container 3 form a closed cavity 4, a refrigerant is filled in the cavity 4, the refrigerant can be isopropanol and can avoid accidents caused by leakage, an injection port 201 communicated with the cavity 4 is formed in the cooling box shell 2, a plug block 202 is arranged in the injection port 201, the refrigerant can be added and replaced after the isopropanol is used for a long time, the plug block 202 is used for sealing, the freezing box is obliquely placed, the refrigerant cannot spill out, leakage is prevented, and the cooling box is more flexible and convenient;

a plurality of grooves 303 are formed in the inner container 3 of the cooling box, and the grooves 303 are used for placing a cryopreservation pipe frame; the cryopreservation pipe frame 5 comprises a placing plate 501, the placing plate 501 is a single layer, a plurality of placing holes 503 are formed in the placing plate 501, and the number of the placing holes 503 is not less than twenty-eight; the lower surface of the placing plate 501 is fixedly connected with the supporting columns 502 matched with the grooves 303, the number of the grooves 303 and the number of the supporting columns 502 are at least two, so that the cryopreservation pipe frame 5 can be separated from the inner container 3 of the cooling box, the cryopreservation pipe frame 5 can be used outside independently, and the problem that the cryopreservation pipe is not easy to stand when placed outside is solved;

a first cylindrical cavity 301 and a second cylindrical cavity 302 which correspond to the placement hole 503 are arranged in the inner container 3 of the cooling box; the number of the first cylindrical cavities 301 is not less than sixteen, the aperture is 13mm, the depth of each hole is 30mm, and the first cylindrical cavities are used for placing 2mL of freezing tubes; the number of the second cylindrical cavities 302 is not less than twelve, the aperture is 13mm, the depth of the holes is 70mm, and the second cylindrical cavities are used for placing 5mL of freezing tubes; the transfer of different models and large batches of frozen pipes can be carried out, so that the time is saved;

the cooling box comprises a box cover 1, wherein a clamping groove 101 or a buckle is arranged on the box cover 1, the box cover 1 is clamped with an annular buckle body 6 through the clamping groove 101 or the buckle, the annular buckle body 6 is embedded in the box cover 1, and the box cover 1 is clamped with a cooling box shell 2 so as to be convenient to disassemble; the box cover 1 is provided with the pull ring 101, the box cover 1 and the pull ring 101 are made of polyethylene PE materials, the contact area of a person and the cooling box is reduced, the carrying and moving are convenient, and the box cover 1 is easy to open.

When using, add the refrigerant to cavity 4 from filling opening 201 to block up with chock 202, prevent revealing, freeze and deposit pillar 502 and the cooling box inner bag 3 that set up on the pipe support 5 and offered a plurality of recess 303 looks adaptations, can make and freeze and deposit pipe support 5 and the separation of cooling box inner bag 3, freeze and deposit pipe support 5 and can use outside alone, and first cylindricality cavity 301 and second cylindricality cavity 302 are in large quantity, can carry out the batch transfer.

In conclusion, the multifunctional program cooling box forms a closed cavity 4 by matching the cooling box shell 2, the cooling box inner container 3 and the annular buckle body 6, and the cavity 4 is filled with the refrigerant, so that the isopropanol can be prevented from leaking to cause safety accidents; in addition, the injection port 201 and the plug 201 which are communicated with the cavity 4 can be added and replaced after the isopropanol is used for a long time, so that the operation is more flexible and convenient.

And, a plurality of recess 303 looks adaptations have been seted up on pillar 502 and the cooling box inner bag 3 that set up on the cryopreserved pipe support 5, can make cryopreserved pipe support 5 and the separation of cooling box inner bag 3, cryopreserved pipe support 5 can use outside alone, the problem of placing the difficult stand of cryopreserved pipe outside has been solved, and the hole 503 of placing that sets up is corresponding with first cylindricality cavity 301 and second cylindricality cavity 302, practiced thrift and to have freezed the pipe and shift to the time of cryopreserving the box in batches, the use is made things convenient for, it is the cavity that the box ectosome and inside cryopreserved pipe support formed to have solved most cooling box isopropyl alcohol hold up tank on the current market, cause need regularly add isopropanol repeatedly and will wait to freeze the in-process that the cell shifts to cryopreserved pipe, the difficult problem of standing and shifting of cryopreserving the pipe.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a multi-functional procedure cooling box which characterized in that: the freezing and storing device comprises a cooling box shell (2), a cooling box inner container (3) and a freezing and storing pipe frame (5) from outside to inside;

the cooling box shell (2) and the cooling box inner container (3) form a closed cavity (4), and a refrigerant is filled in the cavity (4); the cooling box shell (2) and the cooling box inner container (3) are sealed through an annular buckle body (6), and the annular buckle body (6) is hermetically connected to openings at the top ends of the cooling box shell (2) and the cooling box inner container (3);

the freezing pipe frame (5) is placed on the inner container (3) of the cooling box;

still include lid (1), lid (1) detain body (6) and cooling box shell (2) joint through the annular.

2. A multi-functional procedural cooling cartridge of claim 1, wherein: the freezing storage pipe frame (5) comprises a placing plate (501) and a plurality of supporting columns (502), wherein the placing plate (501) is provided with more than twenty-eight placing holes (503); the supporting column (502) is fixedly connected to the lower surface of the placing plate (501).

3. A multi-functional procedural cooling cartridge of claim 2, wherein: seted up on cooling box inner bag (3) with pillar (502) assorted recess (303), recess (303) are used for placing cryopreserving pipe support (5).

4. A multi-functional procedural cooling cartridge of claim 2, wherein: be equipped with in cooling box inner bag (3) and be used for placing 2mL freeze first cylindricality cavity (301) of depositing the pipe and be used for placing 5mL freeze second cylindricality cavity (302) of depositing the pipe, the position one-to-one of the position of first cylindricality cavity (301) and second cylindricality cavity (302) and the position of placing hole (503), sixteen is no less than to the quantity of first cylindricality cavity (301).

5. A multi-functional procedural cooling cartridge of claim 1, wherein: an injection port (201) communicated with the cavity (4) is formed in the cooling box shell (2), and a plug block (202) is matched in the injection port (201).