CN214841876U - Temperature-controllable liquid nitrogen device - Google Patents

Abstract

The utility model discloses a controllable temperature liquid nitrogen device, including the box that the top is uncovered and the case lid that sets up with the box is sealed, be equipped with the relief valve on the case lid, the four corners department of the inner chamber of box is provided with the scale mark respectively, and one side of each scale mark all is equipped with stand I, and the cover is equipped with a ring on each stand I, the internal diameter of ring is greater than the diameter of stand I, the ring is connected with a float through the connecting rod, the inboard of two relative lateral walls of box 1 is equipped with two stands II respectively, all is provided with the scale on each stand II, and the surface of each stand II is equidistant to be provided with a draw-in groove, detachable card establishes a horizontal setting's horizontal pole between the adjacent draw-in grooves, the equidistant slotted hole that is used for placing the test tube that is provided with of upper surface of horizontal pole; the utility model discloses a control the height of depositing of vascular or cryovial, realize the quick, accurate freezing of interior sperm of vascular or cryovial, ovum or stem cell.

Description

Temperature-controllable liquid nitrogen device

Technical Field

The utility model relates to a cold-stored technical field of stem cell specifically relates to a controllable temperature liquid nitrogen device.

Background

Cryopreservation of cells is of increasing importance in the fields of biology, medicine, livestock reproduction, wildlife protection and reproductive medicine, for the short-term or long-term preservation of sperm, ova, embryos, stem cells and other cell lines, the most common and safest cryopreservation method being cryopreservation with liquid nitrogen.

The rapid freezing method, namely, the sample is rapidly cooled in the liquid nitrogen gas phase and then stored in the liquid nitrogen liquid phase, is favored due to simple operation, short time consumption and no need of expensive freezing instruments, but different species and types of cells need different freezing rates, so the distance between the sample and the liquid nitrogen surface in the liquid nitrogen gas phase needs to be considered during rapid freezing, and the larger the distance is, the higher the temperature is. At present, the sample is many direct storages in the liquid nitrogen tank when the storage is cold-stored, and conventional liquid nitrogen tank adopts the tubbiness structure usually, and the setting of this kind of barreled structure often can not accurate controlled temperature to can not carry out the freezing of different speed to the cell of different species and type, consequently in order to compensate above-mentioned not enough, the utility model provides a controllable temperature liquid nitrogen device can with treat the cell accurate control refrigeration condition of depositing in the liquid nitrogen tank in advance, and then realize quick freezing.

SUMMERY OF THE UTILITY MODEL

The utility model provides a solve above-mentioned technical problem, provide a controllable temperature liquid nitrogen device, its simple structure, through the height of depositing of control pulse pipe or cryovial, and then realize quick, accurate freezing of interior sperm of pulse pipe or cryovial, ovum or stem cell.

In order to realize the technical purpose, the utility model discloses the technical scheme who adopts is: the utility model provides a controllable temperature liquid nitrogen device, includes the uncovered box that sets up in top and the case lid that sets up with the box is sealed, be equipped with the relief valve on the case lid, the four corners department of the inner chamber of box is provided with the scale mark respectively, and one side of every scale mark all is equipped with stand I, and the cover is equipped with a ring on every stand I, the internal diameter of ring is greater than the diameter of stand I, the ring is connected with a float through the connecting rod, the inboard of two relative lateral walls of box 1 is equipped with two stands II respectively, all is provided with the scale on every stand II, and the surface equidistant of every stand II is provided with a draw-in groove, and the horizontal pole that a level set up is established to detachable card between the adjacent draw-in groove, the equidistant slotted hole that is used for placing pulse pipe or cryovial that is provided with of upper surface of horizontal pole.

Optionally, the start scale of the tick mark is 0.

Optionally, the distance between adjacent card slots is 1 cm.

The utility model has the advantages that:

1. the utility model is provided with the box body and the end cover which is connected with the box body in a sealing way, can realize sealing through the matching between the box body and the end cover, and is also provided with the relief valve on the box cover, thereby effectively relieving pressure; 2. the utility model discloses a four corners inner wall department is equipped with the scale mark, and the position of adjacent scale mark is equipped with stand I, and the float is installed through the connecting piece to stand I, can directly read out the liquid level height through the cooperation setting of float and scale mark;

3. the utility model discloses center pillar II is equipped with the draw-in groove on every scale, and the horizontal pole card that is used for placing the pulse tube or freezer pipe is established in the draw-in groove, can rationally adjust the height of horizontal pole according to the height of pulse tube or freezer pipe and the kind of the sample that waits to freeze to control the pulse tube or freezer pipe apart from the height of liquid level, and then control and deposit the temperature, in order to realize freezing fast and deposit, in order to satisfy the requirement of depositing of pulse tube or freezer pipe;

4. the utility model discloses the interval is provided with the recess that is used for placing pulse tube or cryovial on the well horizontal pole, can realize the effective location to pulse tube or cryovial through the setting of recess, avoids pulse tube or cryovial to take place to empty or reveal at freezing in-process to the standard of realization pulse tube or cryovial is deposited.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the middle box body of the present invention;

the labels in the figure are: 1. the box body, 2, case lid, 3, scale mark, 4, stand I, 5, ring, 6, connecting rod, 7, float, 8, stand II, 9, draw-in groove, 10, horizontal pole, 11, slotted hole.

Detailed Description

As shown in the figure, the temperature-controllable liquid nitrogen device comprises a box body 1 and a box cover 2, wherein the top of the box body 1 is open, the box cover 2 is sealed with the box body 1, a pressure release valve is arranged on the box cover 2, scale marks 3 are respectively arranged at four corners of an inner cavity of the box body 1, a stand column I4 is arranged on one side of each scale mark 3, a circular ring 5 is sleeved on each stand column I4, the inner diameter of the circular ring 5 is larger than the diameter of the stand column I4, the circular ring 5 is connected with a floater 7 through a connecting rod 6, two stand columns II 8 are respectively arranged on the inner sides of two opposite side walls of the box body 1, scales are arranged on each stand column II 8, a clamping groove 9 is arranged on the outer surface of each stand column II 8 at equal intervals, the stand columns II 8 are arranged to be cuboid stand columns, the clamping grooves 9 are arranged at the positions where the scales are marked on the cuboid stand columns, and a bayonet is arranged on each surface of the scales, the four buckles are communicated to form a buckle groove 9, horizontal cross rods 10 can be clamped between the buckles at the opposite positions of the buckle groove 9, and slotted holes 11 for placing vessels or freezing pipes are arranged on the upper surface of each cross rod 10 at equal intervals.

In some exemplary embodiments, the start scale of the graduation mark 3 is 0.

In some exemplary embodiments, the distance between adjacent card slots 9 is 1 cm.

In some exemplary embodiments, a seal ring is disposed between the case body and the case cover.

The utility model discloses a theory of operation: the utility model discloses in the course of the work, at first liquid nitrogen liquid level height and according to the height of treating the high reasonable adjustment horizontal pole of refrigerated pulse pipe or cryovial apart from the liquid level, after the high definite completion, establish the horizontal pole card on suitable scale height, then will treat that the pulse pipe or the cryovial of depositing places in the horizontal pole slotted hole, according to depositing the standard liquid nitrogen of pouring, at the in-process of pouring the liquid nitrogen, the float can follow the high rise of liquid level, the staff can be according to the surveyed liquid nitrogen volume in the box of the scale mark surveyability that the float corresponds, thereby standardize and deposit, the liquid nitrogen is added and is accomplished the back and cover the case lid and accomplish sealedly.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the technical idea of the present invention to replace or change equally, all should be covered in the protection scope of the present invention.

Claims (3)

1. The utility model provides a controllable temperature liquid nitrogen device, includes open box (1) that sets up in top and case lid (2) that set up with box (1) is sealed, be equipped with the relief valve on case lid (2), its characterized in that: the four corners department of the inner chamber of box (1) is provided with scale mark (3) respectively, and one side of every scale mark (3) all is equipped with stand I (4), and the cover is equipped with a ring (5) on every stand I (4), the internal diameter of ring (5) is greater than the diameter of stand I (4), ring (5) are connected with a float (7) through connecting rod (6), the inboard of two relative lateral walls of box (1) is equipped with two stand II (8) respectively, all is provided with the scale on every stand II (8), and the surface of every stand II (8) is equidistant to be provided with a draw-in groove (9), and horizontal pole (10) that a level set up are established to detachable card between adjacent draw-in groove (9), the upper surface of horizontal pole (10) is equidistant to be provided with a slotted hole (11) that are used for placing the test tube.

2. A temperature-controlled liquid nitrogen apparatus as claimed in claim 1, wherein: the starting scale of the scale mark (3) is 0.

3. A temperature-controlled liquid nitrogen apparatus as claimed in claim 1, wherein: the distance between adjacent clamping grooves (9) is 1 cm.

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