CN220756323U - Program cryopreservation box for gradient cryopreservation of cell suspension - Google Patents
Program cryopreservation box for gradient cryopreservation of cell suspension Download PDFInfo
- Publication number
- CN220756323U CN220756323U CN202322430742.5U CN202322430742U CN220756323U CN 220756323 U CN220756323 U CN 220756323U CN 202322430742 U CN202322430742 U CN 202322430742U CN 220756323 U CN220756323 U CN 220756323U
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- Prior art keywords
- pipe
- cryopreservation
- box body
- freezing
- box
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- 239000006285 cell suspension Substances 0.000 title claims abstract description 18
- 238000005138 cryopreservation Methods 0.000 title claims description 16
- 230000008014 freezing Effects 0.000 claims abstract description 39
- 238000007710 freezing Methods 0.000 claims abstract description 31
- 230000000149 penetrating effect Effects 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 6
- 238000009434 installation Methods 0.000 abstract description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- 239000000110 cooling liquid Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000001816 cooling Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
Abstract
The utility model discloses a program freezing box for gradient freezing of cell suspension, which relates to the technical field of program freezing boxes and comprises a box body and a box cover covered above the box body, wherein a pipe support is inserted into the box body, the pipe support is inserted into the outer wall of a vertical pipe, and the vertical pipe is fixedly arranged on the inner bottom wall of the box body; the pipe support is square structure, and the lateral wall of pipe support runs through and has seted up the frozen tube hole of multiunit different aperture. According to the utility model, the pipe frame is arranged in the box body, under the effect that the pipe frame is square, a user can face the freezing pipe holes with different apertures through overturning the pipe frame, so that the user can conveniently place different freezing pipes according to the use requirement, the practicability and the flexibility of the program freezing box are improved, the operation is simple and convenient, meanwhile, the stability of the installation of the pipe frame is ensured by inserting the pipe frame on the outer wall of the vertical pipe, and the flexible and stable setting of the pipe frame is realized.
Description
Technical Field
The utility model relates to the technical field of program cryopreservation boxes, in particular to a program cryopreservation box for gradient cryopreservation of cell suspension.
Background
The cell suspension is a state that cells are uniformly distributed and suspended in a liquid culture medium, and the cells in the state can freely perform various physiological activities such as division, metabolism and the like, so that the cell suspension has wide application in biological research and medical experiments, along with rapid development of cell biology, the demands of cell culture and freezing research are increased, a variety of modes appear on the specification of a freezing container, particularly the most widely applied gradient cooling box at present, the freezing container provides a repeatable cooling rate of-1 ℃/min, is suitable for various low-temperature pipes and sample bottles, and can provide low-cost cell freezing and restoring.
The utility model discloses a leak protection gradient freezes case of depositing at chinese patent document publication No. CN217242382U, this leak protection gradient freezes case is disclosed including the cold storage case, a plurality of placing bags of cold storage case bottom fixedly connected with, the fixed column is installed to the placing bag bottom surface, the spacing groove has been seted up on the fixed column, install the wedge on the fixed column, a plurality of fixed blocks are installed to the cold storage case bottom surface, be equipped with the spring between fixed block and the fixed column, be equipped with the telescopic link between fixed column and the fixed block, be equipped with the torsional spring on the telescopic link, install a plurality of pairs of supporting seats in the cold storage case, rotate between two supporting seats and be connected with the gag lever post, the gag lever post both sides all are equipped with another torsional spring, placing bag one end is equipped with the rotating block, be equipped with the placing tube in the placing bag, the fixture block is installed to placing tube one end, the cold storage case is equipped with annotate the liquid pipe one end and is equipped with the leak protection cap, cold storage case top is equipped with the case lid, effectually avoid liquid to leak, further ensure the freezing effect of article.
The gradient cryopreservation box can effectively protect the placing pipe, but when in actual use, different cryopreservation pipes can be used due to the difference of cell suspensions, the gradient cryopreservation box can only be matched with the cryopreservation pipe with a single size for use when in use, the flexibility is low, the application range is limited, and the gradient cryopreservation box is inconvenient to operate and has poor practicability due to the fact that cooling liquid is added through the liquid injection pipe at one side.
Disclosure of Invention
The utility model aims to solve the defects that when the conventional gradient freezing box is actually used, different freezing pipes are used due to different cell suspensions, the freezing box can only be used with a freezing pipe with a single size, the flexibility is low, the application range is limited, and the freezing box is inconvenient to operate and poor in practicability due to the fact that cooling liquid is added through a liquid injection pipe at one side.
In order to achieve the above purpose, the present utility model adopts the following technical scheme:
the program freezing box for gradient freezing of the cell suspension comprises a box body and a box cover covered above the box body, wherein a pipe frame is inserted into the box body, the pipe frame is inserted into the outer wall of a vertical pipe, and the vertical pipe is fixedly arranged on the inner bottom wall of the box body;
the pipe support is square structure, and the lateral wall of pipe support runs through and has seted up the frozen tube hole of multiunit different apertures.
As a further description of the above technical solution:
the inside of pipe support runs through and has offered the jack that can hold the riser, and the jack corresponds in the axle center department of pipe support and offer.
As a further description of the above technical solution:
a plurality of groups of flow holes are formed in the lower portion of the vertical pipe in a penetrating mode, and the vertical pipe is fixedly arranged at the axis of the box body.
As a further description of the above technical solution:
the top outer wall threaded connection of riser has the sleeve pipe, and the sleeve pipe sets up in the top of pipe support.
As a further description of the above technical solution:
the box body and the box cover are clamped and limited through the protruding points, and the protruding points are fixedly arranged on the surface of the outer side wall of the box body.
In conclusion, by adopting the technical scheme, the utility model has the beneficial effects that:
1. according to the utility model, the pipe frame is arranged in the box body, under the effect that the pipe frame is square, a user can face the freezing pipe holes with different apertures through overturning the pipe frame, so that the user can conveniently place different freezing pipes according to the use requirement, the practicability and the flexibility of the program freezing box are improved, the operation is simple and convenient, meanwhile, the stability of the installation of the pipe frame is ensured by inserting the pipe frame on the outer wall of the vertical pipe, and the flexible and stable setting of the pipe frame is realized.
2. According to the utility model, through arranging the flow holes at the bottom of the vertical pipe, a user can add cooling liquid into the box body through the vertical pipe, and under the action of arranging the sleeve at the top of the vertical pipe, the sleeve can seal the vertical pipe, meanwhile, the sleeve is arranged above the pipe frame, so that the sleeve is convenient for limiting the pipe frame, and the stability of the pipe frame is further improved.
Drawings
FIG. 1 is a schematic perspective view of a program cryopreservation cassette for gradient cryopreservation of cell suspensions according to the present utility model;
FIG. 2 is a schematic side view of a program freezing box for gradient freezing of cell suspension according to the present utility model;
FIG. 3 is a schematic diagram of an exploded view of a program cryopreservation cassette for gradient cryopreservation of cell suspensions according to the present utility model;
fig. 4 is a schematic perspective view of a pipe rack in the implementation.
Legend description:
1. a case body; 2. a box cover; 3. a pipe rack; 4. a riser; 5. a jack; 6. freezing and storing pipe holes; 7. a flow hole; 8. a sleeve; 9. and (5) protruding points.
Detailed Description
The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
Referring to fig. 1-4, a program freezing box for gradient freezing of cell suspension comprises a box body 1, a box cover 2, a pipe support 3, a vertical pipe 4, a jack 5, freezing pipe holes 6, a circulation hole 7, a sleeve 8 and a salient point 9, wherein the box cover 2, the pipe support 3, the vertical pipe 4, the jack 5, the freezing pipe holes 6, the circulation hole 7, the sleeve 8 and the salient point 9 are covered above the box body 1, the clamping limit is carried out between the box body 1 and the box cover 2 through the salient point 9 in consideration of the sealing effect between the box body 1 and the box cover 2, the salient point 9 is fixedly arranged on the surface of the outer side wall of the box body 1, the box body 1 is convenient to stably close the box body 1 above, the box cover 2 is simple and convenient to install, meanwhile, the pipe support 3 is inserted into the box body 1 in consideration of using freezing pipes with different sizes, the pipe support 3 is particularly in a square structure, six sides of the pipe support 3 are identical in size, the box body 1 is convenient to turn and adjust, further, under the effect of the freezing pipe holes 6 with different sizes, a user can adjust the size of the freezing pipe support 3 according to the size of the freezing pipe holes 6, the size of the freezing pipe support 3 is convenient to adjust the size of the freezing pipe support 3, and the freezing pipe support 3 is convenient to adjust the size to the inside the pipe support 3 through the freezing pipe support 6, and the program freezing pipe support 3 is convenient to store the program and convenient to adjust the size and store the size and has different size and practical size and convenient to store the size and has the size and convenient to use;
referring to fig. 1-4, considering the fixing effect of the pipe rack 3 after the inside of the box body 1 is turned over, and in order to improve the stability of the pipe rack 3, the pipe rack 3 is conveniently and flexibly taken and placed by a user, the pipe rack 3 is inserted and arranged on the outer wall of the vertical pipe 4, the vertical pipe 4 is fixedly installed on the inner bottom wall of the box body 1, specifically, the jack 5 capable of accommodating the vertical pipe 4 is penetrated and arranged in the pipe rack 3, and the jack 5 is correspondingly arranged at the axis of the pipe rack 3, through the arrangement, the pipe rack 3 can be inserted and connected on the surface of the vertical pipe 4 through the jack 5, and through arranging the jack 5 on each surface of the pipe rack 3, the pipe rack 3 can be conveniently and fixedly turned over in different directions for the pipe rack 3 through different jacks 5, the flexibility of the pipe rack 3 is improved, the installation of the pipe rack 3 is simple and convenient, and meanwhile, in order to facilitate the user to add cooling liquid into the box body 1, the cooling liquid is preferably isopropanol, a plurality of groups of flow holes 7 are formed below the vertical pipe 4 in a penetrating way, the vertical pipe 4 is fixedly arranged at the axle center of the box body 1, when the user adds isopropanol above through the vertical pipe 4, the isopropanol enters the bottom of the box body 1 through the flow holes 7, the freezing storage pipe inside the pipe rack 3 is convenient to freeze, further, in consideration of the sealing of the vertical pipe 4, the sleeve 8 is connected with the outer wall of the top end of the vertical pipe 4 in a threaded way, the sleeve 8 is convenient to seal at the top end of the vertical pipe 4 through threads, the sleeve 8 is arranged above the pipe rack 3 after the surface of the vertical pipe 4 is inserted, the sleeve 8 can be limited above the pipe rack 3 through the arrangement, the stability of the pipe rack 3 is further improved, and the operation is simple and convenient.
The present utility model is not limited to the above-mentioned embodiments, and any person skilled in the art, based on the technical solution of the present utility model and the inventive concept thereof, can be replaced or changed within the scope of the present utility model.
Claims (5)
1. The utility model provides a procedure cryopreservation box for cell suspension gradient cryopreservation, includes box body (1) and lid (2) of lid in box body (1) top, its characterized in that: the inner part of the box body (1) is inserted with a pipe rack (3), the pipe rack (3) is inserted with the outer wall of the vertical pipe (4), and the vertical pipe (4) is fixedly arranged on the inner bottom wall of the box body (1);
the pipe support (3) is of a cube structure, and the side wall of the pipe support (3) is penetrated and provided with a plurality of groups of freezing pipe holes (6) with different apertures.
2. A program cryopreservation cassette for gradient cryopreservation of cell suspensions according to claim 1, wherein: the inside of pipe support (3) runs through and has offered jack (5) that can hold riser (4), and jack (5) are offered in the axle center department of pipe support (3) correspondence.
3. A program cryopreservation cassette for gradient cryopreservation of cell suspensions according to claim 2, wherein: a plurality of groups of flow holes (7) are formed in the lower portion of the vertical pipe (4) in a penetrating mode, and the vertical pipe (4) is fixedly arranged at the axis of the box body (1).
4. A program cryopreservation cassette for gradient cryopreservation of cell suspensions according to claim 3, wherein: the top end outer wall thread of the vertical pipe (4) is connected with a sleeve (8), and the sleeve (8) is arranged above the pipe frame (3).
5. A program cryopreservation cassette for gradient cryopreservation of cell suspensions according to claim 1, wherein: the box body (1) and the box cover (2) are clamped and limited through the protruding points (9), and the protruding points (9) are fixedly arranged on the surface of the outer side wall of the box body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322430742.5U CN220756323U (en) | 2023-09-07 | 2023-09-07 | Program cryopreservation box for gradient cryopreservation of cell suspension |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202322430742.5U CN220756323U (en) | 2023-09-07 | 2023-09-07 | Program cryopreservation box for gradient cryopreservation of cell suspension |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220756323U true CN220756323U (en) | 2024-04-12 |
Family
ID=90616022
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202322430742.5U Active CN220756323U (en) | 2023-09-07 | 2023-09-07 | Program cryopreservation box for gradient cryopreservation of cell suspension |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220756323U (en) |
-
2023
- 2023-09-07 CN CN202322430742.5U patent/CN220756323U/en active Active
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