CN206799487U - A kind of heat-preservation fixing device of protein purification post - Google Patents
A kind of heat-preservation fixing device of protein purification post Download PDFInfo
- Publication number
- CN206799487U CN206799487U CN201720547478.5U CN201720547478U CN206799487U CN 206799487 U CN206799487 U CN 206799487U CN 201720547478 U CN201720547478 U CN 201720547478U CN 206799487 U CN206799487 U CN 206799487U
- Authority
- CN
- China
- Prior art keywords
- heat
- purification column
- seal case
- post
- purification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001742 protein purification Methods 0.000 title claims abstract description 21
- 238000004321 preservation Methods 0.000 title claims abstract description 14
- 238000000746 purification Methods 0.000 claims abstract description 52
- 238000009413 insulation Methods 0.000 claims abstract description 20
- 229920005479 Lucite® Polymers 0.000 claims abstract description 7
- 239000004926 polymethyl methacrylate Substances 0.000 claims abstract description 7
- 239000012774 insulation material Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims 2
- 229910021529 ammonia Inorganic materials 0.000 claims 1
- 150000002148 esters Chemical class 0.000 claims 1
- 238000010276 construction Methods 0.000 abstract description 2
- 102000004169 proteins and genes Human genes 0.000 description 10
- 108090000623 proteins and genes Proteins 0.000 description 10
- 230000000694 effects Effects 0.000 description 7
- 229920002635 polyurethane Polymers 0.000 description 7
- 239000004814 polyurethane Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 3
- 238000007710 freezing Methods 0.000 description 3
- 230000008014 freezing Effects 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 239000002826 coolant Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000010926 purge Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001788 irregular Effects 0.000 description 1
- 230000006386 memory function Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
It the utility model is related to a kind of heat-preservation fixing device of protein purification post, including seal case, movable flip is connected with above seal case, the front portion of seal case is removably provided with lucite, heat-insulation layer is nested with the inwall of seal case, both sides inwall against heat-insulation layer is provided with retractable support post, the upper horizontal of retractable support post is supported by purification column fixed plate, the lower section of purification column fixed plate is provided with the centrifuge tube shelf with the horizontal clamping of retractable support post, purification column fixed plate is provided with multiple purification column fixing holes, centrifuge tube shelf is provided with centrifuge tube shelf fixing hole corresponding with purification column fixing hole, the interior bottom wall of seal case is provided with cushion.The utility model can conveniently and efficiently realize the vertical fixation of multiple protein purification posts, the height of purification column fixed plate is adjusted to meet the requirement of different length purification column by adjusting the height of retractable support post, seal case inwall sets heat-insulation layer to realize that albumen is incubated store function, it is simple in construction, it is easy to use.
Description
Technical field
The utility model belongs to bio-instruments technical field, more particularly to a kind of heat-preservation fixing device of protein purification post.
Background technology
Isolating and purifying for protein uses extensively in biochemical research application, is an important operating technology.One
Individual typical eukaryotic can include thousands of different proteins, wherein, some content very abundants, and some only contain
There are several copies.In order to study some protein, it is necessary to first by the protein from other protein and non-proteinaceous molecule
In be purified.Essential experiment equipment is exactly purification column during the isolating and purifying of protein.
At present, the shape for the protein purification post that laboratory uses is not the solid of rule, and this purification column is difficult to directly
Place on the table, when being fixed according in general rack for test tube, can only once fix a purification column, it is fixed difficult and difficult
To keep purification column straight down;In addition, the protein being temporarily attached in purification column is easy to because ambient temperature is too high
And loss of activity, so as to cause the failure of experiment.
Utility model content
The utility model for solve known technology present in because protein purification post it is in irregular shape be difficult to directly put
Put on the table, when being fixed according in general rack for test tube, can only once fix a purification column, fix difficult and be difficult to
Keep purification column straight down, the protein being temporarily attached in purification column be easy to because ambient temperature is too high and loss of activity,
So as to cause the technical problem of the failure of an experiment and provide it is a kind of it is easy to use, can efficiently realize the perpendicular of multiple protein purification posts
Straight fixed, the height of purification column fixed plate can be adjusted according to purifying column length, have the albumen of albumen insulation memory function
The heat-preservation fixing device of purification column.
The utility model is adopted the technical scheme that to solve technical problem present in known technology:
A kind of heat-preservation fixing device of protein purification post, including seal case, work is connected with above the seal case
Dynamic to renovate, the front portion of the seal case is removably provided with lucite, and guarantor is nested with the inwall of the seal case
Warm layer, retractable support post is installed against the both sides inwall of the heat-insulation layer, the upper horizontal of the retractable support post is supported by
Purification column fixed plate, the lower section of the purification column fixed plate is provided with the centrifuge tube shelf with the horizontal clamping of retractable support post, described pure
Change post fixed plate and be provided with purification column fixing hole, the centrifuge tube shelf is provided with centrifuge tube corresponding with the purification column fixing hole
Frame fixing hole, the interior bottom wall of the seal case are provided with cushion.
Preferably, the purification column fixing hole is provided with multiple.
Preferably, the cushion is provided with arc groove corresponding with centrifuge tube shelf fixing hole.
Preferably, the vertex of top one of the seal case is provided with filling hole, the lower section of the seal case and filling
The catercorner vertex in hole is provided with tap, and matching sealing-plug is respectively equipped with the filling hole and tap.
Preferably, filled polyurethane insulation material in the heat-insulation layer.
The utility model has the advantages and positive effects of:
(1) the utility model utilizes the polyurethane for filling hole filling freezing processing by setting seal case and heat-insulation layer
Insulation material is done cooling agent and the mode of the cooling agent is discharged using tap, and interim refrigerating box, energy are formed with reference to heat-insulation layer
It is enough effectively prevent protein in purge process because environment temperature is too high and loss of activity, high insulating effect and simple in construction.
(2) by the horizontally disposed purification column fixed plate with multiple purification column fixing holes, can one-time fix multiple
Purification column, and purification column can be ensured straight down, make purification column fixation procedure convenient and swift.
(3) set retractable support post to support purification column fixed plate, can be adjusted by the height for adjusting retractable support post
The height of purification column fixed plate, it is easy to use to meet the requirement of different length purification column.
(4) by setting centrifuge tube shelf, centrifuge tube shelf fixing hole and arc groove, centrifuge tube can be effectively fixed, for containing
Protein purification liquid is put, it is rational in infrastructure.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the heat-preservation fixing device for protein purification post that the utility model embodiment provides.
Fig. 2 is the structural representation for the purification column fixed plate that the utility model embodiment provides.
In figure:1- seal cases;2- movable flips;3- lucite;4- purification column fixed plates;5- centrifuge tube shelfs;6- is pure
Change post fixing hole;7- centrifuge tube shelf fixing holes;8- retractable support posts;9- cushions;10- arc grooves;11- heat-insulation layers;12- is filled out
Fill hole;13- taps;14- sealing-plugs.
Embodiment
For invention, features and effects of the present utility model can be further appreciated that, following examples are hereby enumerated, and coordinate
Accompanying drawing describes in detail as follows.
Detailed retouch is made to the structure of the heat-preservation fixing device of the utility model protein purification post with reference to Fig. 1-Fig. 2
State.
A kind of heat-preservation fixing device of protein purification post, including seal case 1, the top of the seal case 1 are connected with
Movable flip 2, the front portion of the seal case 1 is dismountable to be provided with lucite 3, embedding on the inwall of the seal case 1
Heat-insulation layer 11 is cased with, the both sides inwall of the heat-insulation layer 11 is provided with retractable support post 8, the top water of the retractable support post 8
Flat to be supported by purification column fixed plate 4, the lower section of the purification column fixed plate 4 is provided with the centrifugation with the 8 horizontal clamping of retractable support post
Pipe support 5, the purification column fixed plate 4 are provided with purification column fixing hole 6, and the centrifuge tube shelf 5 is provided with to be consolidated with the purification column
Determine centrifuge tube shelf fixing hole 7 corresponding to hole 6, the interior bottom wall of the seal case 1 is provided with cushion 9.
The purification column fixing hole 6 be provided with it is multiple, multiple purification columns can be one-time fixed.
The cushion 9 is provided with arc groove 10 corresponding with centrifuge tube shelf fixing hole 7, is easy to effectively fixed centrifugation
Pipe, for holding protein purification liquid.
The vertex of top one of the seal case 1 is provided with filling hole 12, lower section and the filling hole 12 of the seal case 1
A catercorner vertex is provided with tap 13, and matching sealing is respectively equipped with the filling hole 12 and tap 13
Plug 14.
Filled polyurethane insulation material in the heat-insulation layer 11, polyurethane heat insulation material insert heat-insulation layer by filling hole 12
In 11, polyurethane heat insulation material can exclude heat-insulation layer 11 by tap 13.
In use, opening movable flip 2, the polyurethane heat insulation material of freezing processing is filled in heat-insulation layer 11, filling is completed
Afterwards, fill and sealing-plug 14 is filled in hole 12 and tap 13, dismantle the operation that lower lucite 3 is easy to protein purification procedures, will
Centrifuge tube is placed in centrifuge tube shelf fixing hole 7, and purification column is placed in purification column fixing hole 6, due to the horizontal branch of purification column fixed plate 6
Support on retractable support post 8, purification column can be ensured straight down, fix purification column quick and easy, then according to purification column
Length adjustment retractable support post 8 height, make between purification column bottom and centrifuge tube upper end be adapted, protein purification has operated
Cheng Hou, lucite 3 is installed, covers tightly movable flip 2.By the side that the polyurethane heat insulation material after freezing is filled in heat-insulation layer 11
Formula, whole device is formed an interim refrigerating box, can effectively prevent protein in purge process because environment temperature is too high
And loss of activity, reach heat insulation effect.
Described above is only to preferred embodiment of the present utility model, not the utility model is made any formal
Limitation, it is every according to the technical essence of the utility model to any simple modification made for any of the above embodiments, equivalent variations with
Modification, is belonged in the range of technical solutions of the utility model.
Claims (5)
- A kind of 1. heat-preservation fixing device of protein purification post, it is characterised in that including seal case, the top of the seal case It is connected with movable flip, the front portion of the seal case is dismountable to be provided with lucite, on the inwall of the seal case Heat-insulation layer is nested with, retractable support post, the top water of the retractable support post are installed against the both sides inwall of the heat-insulation layer Flat to be supported by purification column fixed plate, the lower section of the purification column fixed plate is provided with the centrifuge tube with the horizontal clamping of retractable support post Frame, the purification column fixed plate are provided with purification column fixing hole, and the centrifuge tube shelf is provided with and the purification column fixing hole pair The centrifuge tube shelf fixing hole answered, the interior bottom wall of the seal case are provided with cushion.
- 2. the heat-preservation fixing device of protein purification post as claimed in claim 1, it is characterised in that the purification column fixing hole is provided with It is multiple.
- 3. the heat-preservation fixing device of protein purification post as claimed in claim 1, it is characterised in that the cushion be provided with from Arc groove corresponding to heart pipe support fixing hole.
- 4. the heat-preservation fixing device of protein purification post as claimed in claim 1, it is characterised in that the top one of the seal case Vertex is provided with filling hole, and lower section and the filling catercorner vertex in hole of the seal case are provided with tap, described Matching sealing-plug is respectively equipped with filling hole and tap.
- 5. the heat-preservation fixing device of protein purification post as claimed in claim 1, it is characterised in that poly- ammonia is filled in the heat-insulation layer Ester insulation material.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201720547478.5U CN206799487U (en) | 2017-05-17 | 2017-05-17 | A kind of heat-preservation fixing device of protein purification post |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201720547478.5U CN206799487U (en) | 2017-05-17 | 2017-05-17 | A kind of heat-preservation fixing device of protein purification post |
Publications (1)
Publication Number | Publication Date |
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CN206799487U true CN206799487U (en) | 2017-12-26 |
Family
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CN201720547478.5U Expired - Fee Related CN206799487U (en) | 2017-05-17 | 2017-05-17 | A kind of heat-preservation fixing device of protein purification post |
Country Status (1)
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CN (1) | CN206799487U (en) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021120610A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Highly-adjustable chromatography column temperature equalizer |
CN113713878A (en) * | 2021-09-13 | 2021-11-30 | 深圳市雅为泓源生物科技有限公司 | Device for fixing and controlling temperature of purification column |
-
2017
- 2017-05-17 CN CN201720547478.5U patent/CN206799487U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2021120610A1 (en) * | 2020-07-13 | 2021-06-24 | 南通大学 | Highly-adjustable chromatography column temperature equalizer |
CN113713878A (en) * | 2021-09-13 | 2021-11-30 | 深圳市雅为泓源生物科技有限公司 | Device for fixing and controlling temperature of purification column |
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Legal Events
Date | Code | Title | Description |
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GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171226 |
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CF01 | Termination of patent right due to non-payment of annual fee |