CN203672691U - Pressure plate type antigen retrieval frame for suspended brain slice - Google Patents
Pressure plate type antigen retrieval frame for suspended brain slice Download PDFInfo
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- CN203672691U CN203672691U CN201320873959.7U CN201320873959U CN203672691U CN 203672691 U CN203672691 U CN 203672691U CN 201320873959 U CN201320873959 U CN 201320873959U CN 203672691 U CN203672691 U CN 203672691U
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Abstract
The utility model relates to a pressure plate type antigen retrieval frame for a suspended brain slice in the field of medical experimental devices. The frame consists of slice pressing clamps (1) and a carrying basket (2), which are combined, wherein each slice pressing clamp (1) consists of a rectangular frame (3) and a rectangular base frame (4); four flow guide grooves (7) are formed in the four sides of each frame (3) and the corresponding base frame (4); two cross rods (8) and two groove support rods (10) form the open surface of the carrying basket (2); four cross rods (8) form the bottom surface of the carrying basket (2); a groove support rod (10) is mounted in the middle of the bottom surface of the carrying basket (2); four vertical rods (9) are connected with the open surface and bottom surface of the carrying basket (2) respectively. According to the pressure plate type antigen retrieval frame, the defect of high shrinking possibility of a suspended brain slice in the existing thermal retrieval process is overcome; the pressure plate type antigen retrieval frame is simple in structure and convenient to operate, and can be used for the antigen retrieval of the suspended brain slice while ensuring no shrinking of the brain tissue slice.
Description
Affiliated technical field
The utility model relates to medical experiment device field, is specially a kind of presser type suspension brain sheet antigen retrieval frame.
Background technology
Suspension brain sheet is one of tissue samples conventional in Neuroscience Research process, is used for nervous system morphology, pathological correlative study.Brain tissue sample is in manufacturing process, because antigen has been sealed in the effect of chemical reagent, because causing the peptide chain of incomplete antigen, hot effect is distorted again, cause in the dyeing course of SABC and can not display it, in order to solve the above problems, utilize hot effect that these antigens are again come out or revised, i.e. antigen retrieval.Antigen retrieval technology is one of experimental technique conventional in the research work such as clinical examination or morphology research, can greatly improve the positive rate of SABC or immunofluorescence.Wherein, " the hot repairing method of micro-wave oven " is the antigen retrieval method of commonly using the most, being convenient to extensively carry out most.But hot repair process easily causes suspension brain sheet generation shrinkage, has a strong impact on follow-up research.
Summary of the invention
The purpose of this utility model is easily to cause the defect of suspension brain sheet generation shrinkage in order to overcome existing hot repair process, provide a kind of simple in structure, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can ensure that again the presser type suspension brain sheet antigen retrieval frame of shrinkage did not occur brain tissue slice.
The purpose of this utility model realizes by following technical proposals:
Presser type suspension brain sheet antigen retrieval frame of the present utility model, constituted by piece pressing clip (1) and hand basket (2), it is characterized in that piece pressing clip (1) is made up of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5), framework (3) is pressed in the base groove (6) matching with framework (3) on bedframe (4), four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on diversion trench (7) and bedframe (4) on framework (3) matches, hand basket (2) is by six roots of sensation cross bar (8), four vertical rods (9) and three groove poles (10) form, wherein, the opening surface of two cross bars (8) and two groove poles (10) composition hand baskets (2), the bottom surface of four cross bars (8) composition hand baskets (2), a groove pole (10) is arranged in the middle of hand basket (2) bottom surface, parallel with the two groove poles (10) on opening surface, groove (11) on three groove poles (10) is mutually corresponding, in order to hold piece pressing clip (1), four vertical rods (9) are connected with bottom surface with the opening surface of hand basket (2) respectively.
In such scheme, on described base groove (6), have slide groove (12).
In such scheme, described piece pressing clip (1) is placed in the groove (11) of hand basket (2) upper groove pole (10).
Use procedure of the present utility model is as follows:
In PBS solution (phosphate buffered solution), " suspension brain sheet " is laid on microslide, cover after another sheet microslide, be placed in the slide groove on bedframe, cover framework, rotary tight adjustable nut, clamps microslide.The piece pressing clip that accompanies suspension brain sheet is inserted in the groove of hand basket one by one, form assembly entirety immersion afterwards and fill with in the beaker of sodium citrate antigen retrieval liquid, in micro-wave oven, heat, high fire 5 minutes, middle height fire 20 minutes.Then at room temperature, naturally cooling.After cooling, take out suspension brain sheet piece pressing clip, in PBS solution, under little writing brush auxiliary, take out from microslide the brain sheet suspending after repairing, for follow-up morphology research.
The utlity model has following advantage: simple in structure, easily manufacture, easy to operate, effect stability, both can realize the antigen retrieval of " suspension brain sheet ", can ensure that again shrinkage does not occur brain tissue slice.
Therefore, the utility model has overcome existing hot repair process easily causes the defect of suspension brain sheet generation shrinkage, and the presser type suspension brain sheet antigen retrieval shelf structure providing is simple, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can ensure that again shrinkage did not occur brain tissue slice.
Brief description of the drawings
Fig. 1 is schematic diagram of the present utility model.
Fig. 2 is piece pressing clip schematic diagram of the present utility model.
Fig. 3 is hand basket schematic diagram of the present utility model.
In accompanying drawing, the implication of each numeral is: 1: piece pressing clip; 2: hand basket; 3: framework; 4: bedframe; 5: adjustable nut; 6: base groove; 7: diversion trench; 8: cross bar; 9: vertical rod; 10: groove pole; 11: groove; 12: slide groove; 13: screw; 14: hold-down nut.
Embodiment
Be described in further detail the utility model below in conjunction with drawings and Examples, but the utility model is not limited only to described embodiment.
Embodiment mono-
The presser type suspension brain sheet antigen retrieval frame that this is routine, as shown in Figure 1, Figure 2 and Figure 3, constituted by piece pressing clip 1 and hand basket 2, it is characterized in that piece pressing clip 1 is made up of rectangular framework 3 and rectangular bedframe 4, by adjustable nut 5, upper frame 3 is pressed in the base groove 6 matching with framework 3 on bedframe 4; Four limits of framework 3 and four limits of bedframe 4 have diversion trench 7, and the diversion trench 7 on diversion trench 7 and bedframe 4 on framework 3 matches; Hand basket 2 is made up of six roots of sensation cross bar 8, four vertical rods 9 and three groove poles 10, wherein, two cross bars 8 and two groove poles 10 form the opening surface of hand basket 2, four cross bars 8 form the bottom surface of hand basket 2, a groove pole 10 is arranged in the middle of hand basket 2 bottom surfaces, parallel with two groove poles 10 on opening surface, and the groove 11 on three groove poles 10 is mutually corresponding, be connected with bottom surface with the opening surface of hand basket 2 respectively in order to hold 1, four vertical rod 9 of piece pressing clip.
On base groove 6, have slide groove 12.
Piece pressing clip 1 is placed in the groove 11 of hand basket 2 upper groove poles 10.
Claims (3)
1. a presser type suspension brain sheet antigen retrieval frame, constituted by piece pressing clip (1) and hand basket (2), it is characterized in that piece pressing clip (1) is made up of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5), framework (3) is pressed in the base groove (6) matching with framework (3) on bedframe (4), four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on diversion trench (7) and bedframe (4) on framework (3) matches, hand basket (2) is by six roots of sensation cross bar (8), four vertical rods (9) and three groove poles (10) form, wherein, the opening surface of two cross bars (8) and two groove poles (10) composition hand baskets (2), the bottom surface of four cross bars (8) composition hand baskets (2), a groove pole (10) is arranged in the middle of hand basket (2) bottom surface, parallel with the two groove poles (10) on opening surface, groove (11) on three groove poles (10) is mutually corresponding, in order to hold piece pressing clip (1), four vertical rods (9) are connected with bottom surface with the opening surface of hand basket (2) respectively.
2. presser type suspension brain sheet antigen retrieval frame according to claim 1, is characterized in that having on described base groove (6) slide groove (12).
3. presser type suspension brain sheet antigen retrieval frame according to claim 1, is characterized in that described piece pressing clip (1) is placed in the groove (11) of hand basket (2) upper groove pole (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320873959.7U CN203672691U (en) | 2013-12-29 | 2013-12-29 | Pressure plate type antigen retrieval frame for suspended brain slice |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320873959.7U CN203672691U (en) | 2013-12-29 | 2013-12-29 | Pressure plate type antigen retrieval frame for suspended brain slice |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203672691U true CN203672691U (en) | 2014-06-25 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201320873959.7U Expired - Fee Related CN203672691U (en) | 2013-12-29 | 2013-12-29 | Pressure plate type antigen retrieval frame for suspended brain slice |
Country Status (1)
| Country | Link |
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| CN (1) | CN203672691U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712837A (en) * | 2013-12-29 | 2014-04-09 | 中国人民解放军成都军区总医院 | Suspended brain slice antigen retrieval pressing clamp |
| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
-
2013
- 2013-12-29 CN CN201320873959.7U patent/CN203672691U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103712837A (en) * | 2013-12-29 | 2014-04-09 | 中国人民解放军成都军区总医院 | Suspended brain slice antigen retrieval pressing clamp |
| CN103712837B (en) * | 2013-12-29 | 2015-10-28 | 中国人民解放军成都军区总医院 | Suspension brain sheet antigen retrieval piece pressing clip |
| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140625 Termination date: 20141229 |
|
| EXPY | Termination of patent right or utility model |