CN103712837A - Suspended brain slice antigen retrieval pressing clamp - Google Patents
Suspended brain slice antigen retrieval pressing clamp Download PDFInfo
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- CN103712837A CN103712837A CN201310736656.5A CN201310736656A CN103712837A CN 103712837 A CN103712837 A CN 103712837A CN 201310736656 A CN201310736656 A CN 201310736656A CN 103712837 A CN103712837 A CN 103712837A
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Abstract
The invention relates to a suspended brain slice antigen retrieval pressing clamp which belongs to the field of medical experiment devices. The clamp consists of a rectangular frame (3) and a rectangular base frame (4), wherein the frame (3) is tightly compressed into a base groove (6) in match with the frame (3) in the base frame (4) through adjusting screw nuts (5); guide grooves (7) are formed in the four edges of the frame (3) and the four edges of the base frame (4); the guide grooves (7) in the frame (3) are matched with the guide grooves (7) in the base frame (4). By adopting the clamp, the defect that a suspended brain slice is easy to wrinkle in the conventional thermal retrieval process is overcome, the suspended brain slice antigen retrieval pressing clamp provided by the invention is simple in structure and convenient to operate, the antigen retrieval of the 'suspended bran slice' can be achieved, and a brain tissue section is prevented from wrinkling.
Description
Affiliated technical field
The present invention relates to medical experiment device field, be specially a kind of suspension brain sheet antigen retrieval piece pressing clip.
Background technology
Suspension brain sheet is one of tissue samples conventional in Neuroscience Research process, is used for nervous system morphology, pathological correlative study.Brain tissue sample is in manufacturing process, because antigen has been sealed in the effect of chemical reagent, because causing the peptide chain of incomplete antigen, hot effect is distorted again, cause in the dyeing course of SABC and can not display it, in order to solve the above problems, utilize hot effect that these antigens are again come out or revised, i.e. antigen retrieval.Antigen retrieval technology is one of experimental technique conventional in the research works such as clinical examination or morphology research, can greatly improve the positive rate of SABC or immunofluorescence.Wherein, " the hot repairing method of micro-wave oven " is the antigen retrieval method of commonly using the most, being convenient to extensively carry out most.But hot repair process easily causes suspension brain sheet generation shrinkage, has a strong impact on follow-up research.
Summary of the invention
The object of the invention is easily to cause in order to overcome existing hot repair process the defect of suspension brain sheet generation shrinkage, provide a kind of simple in structure, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can guarantee that again the suspension brain sheet antigen retrieval piece pressing clip of shrinkage did not occur brain tissue slice.
The object of the invention is to realize by following technical proposals:
Suspension brain sheet antigen retrieval piece pressing clip of the present invention, it is characterized in that piece pressing clip (1) is comprised of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5), framework (3) is pressed in the base groove (6) matching with framework (3) on bedframe (4); Four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on framework (3) and the diversion trench (7) on bedframe (4) match.
In such scheme, on described base groove (6), have slide groove (12).
In such scheme, described piece pressing clip (1) is placed in hand basket (2), hand basket (2) is by six roots of sensation cross bar (8), four vertical rods (9) and three groove poles (10) form, wherein, two cross bars (8) and two groove poles (10) form the opening surface of hand basket (2), four cross bars (8) form the bottom surface of hand basket (2), a groove pole (10) is arranged in the middle of hand basket (2) bottom surface, parallel with the two groove poles (10) on opening surface, groove (11) on three groove poles (10) is mutually corresponding, in order to hold piece pressing clip (1), four vertical rods (9) are connected with bottom surface with the opening surface of hand basket (2) respectively.
Use procedure of the present invention is as follows:
In PBS solution (phosphate buffered solution), " suspension brain sheet " is laid on microslide, cover after another sheet microslide, be placed in the slide groove on bedframe, cover framework, rotary tight adjustable nut, microslide is clamped, accompanied the piece pressing clip of " suspension brain sheet ".The piece pressing clip that accompanies suspension brain sheet is directly immersed and filled with in the beaker of sodium citrate antigen retrieval liquid, or the piece pressing clip that accompanies suspension brain sheet is inserted in the groove of hand basket one by one, after forming assembly, whole immersion filled with in the beaker of sodium citrate antigen retrieval liquid, in micro-wave oven, heat, high fire 5 minutes, middle high fire 20 minutes.Then at room temperature, naturally cooling.After cooling, take out suspension brain sheet piece pressing clip, in PBS solution, under little writing brush auxiliary, from microslide, take out the brain sheet suspending after repairing, for follow-up morphology research.
The present invention has the following advantages: simple in structure, easily manufacture, easy to operate, and effect stability both can be realized the antigen retrieval of " suspension brain sheet ", can guarantee that again shrinkage does not occur brain tissue slice.
Therefore, the present invention has overcome the defect that existing hot repair process easily causes suspension brain sheet generation shrinkage, and the suspension brain sheet antigen retrieval piece pressing clip providing is simple in structure, easy to operate, both can realize the antigen retrieval of " suspension brain sheet ", and can guarantee that again shrinkage did not occur brain tissue slice.
Accompanying drawing explanation
Fig. 1 is schematic diagram of the present invention.
Fig. 2 is hand basket schematic diagram of the present invention.
Fig. 3 is piece pressing clip of the present invention and hand basket combination schematic diagram.
In accompanying drawing, each digital implication is: 1: piece pressing clip; 2: hand basket; 3: framework; 4: bedframe; 5: adjustable nut; 6: base groove; 7: diversion trench; 8: cross bar; 9: vertical rod; 10: groove pole; 11: groove; 12: slide groove; 13: screw; 14: hold-down nut.
Embodiment
Below in conjunction with drawings and Examples, be described in further detail the present invention, but the present invention is not limited only to described embodiment.
Embodiment mono-
The suspension brain sheet antigen retrieval piece pressing clip of this example, as shown in Figure 1, is comprised of rectangular framework 3 and rectangular bedframe 4, by adjustable nut 5, upper frame 3 is pressed in the base groove 6 matching with framework 3 on bedframe 4; Four limits of framework 3 and four limits of bedframe 4 have diversion trench 7, and the diversion trench 7 on framework 3 and the diversion trench 7 on bedframe 4 match.
On base groove 6, have slide groove 12.
Embodiment bis-
The suspension brain sheet antigen retrieval piece pressing clip of this example is as Fig. 1, shown in Fig. 2 and Fig. 3, piece pressing clip 1 is with embodiment mono-, hand basket 2 is by six roots of sensation cross bar 8, four vertical rods 9 and three groove poles 10 form, wherein, the opening surface of two cross bars 8 and two groove pole 10 composition hand baskets 2, four cross bars 8 form the bottom surface of hand basket 2, a groove pole 10 is arranged in the middle of hand basket 2 bottom surfaces, parallel with two groove poles 10 on opening surface, groove 11 on three groove poles 10 is mutually corresponding, in order to hold piece pressing clip 1, four vertical rods 9 are connected with bottom surface with the opening surface of hand basket 2 respectively.Piece pressing clip 1 is placed in the groove 11 of hand basket 2 upper groove poles 10.
Claims (3)
1. a suspension brain sheet antigen retrieval piece pressing clip, it is characterized in that piece pressing clip (1) is comprised of rectangular framework (3) and rectangular bedframe (4), by adjustable nut (5), framework (3) is pressed in the base groove (6) matching with framework (3) on bedframe (4); Four limits of framework (3) and four limits of bedframe (4) have diversion trench (7), and the diversion trench (7) on framework (3) and the diversion trench (7) on bedframe (4) match.
2. suspension brain sheet antigen retrieval piece pressing clip according to claim 1, is characterized in that having on described base groove (6) slide groove (12).
3. suspension brain sheet antigen retrieval piece pressing clip according to claim 1, it is characterized in that described piece pressing clip (1) is placed in hand basket (2), hand basket (2) is by six roots of sensation cross bar (8), four vertical rods (9) and three groove poles (10) form, wherein, two cross bars (8) and two groove poles (10) form the opening surface of hand basket (2), four cross bars (8) form the bottom surface of hand basket (2), a groove pole (10) is arranged in the middle of hand basket (2) bottom surface, parallel with the two groove poles (10) on opening surface, groove (11) on three groove poles (10) is mutually corresponding, in order to hold piece pressing clip (1), four vertical rods (9) are connected with bottom surface with the opening surface of hand basket (2) respectively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310736656.5A CN103712837B (en) | 2013-12-29 | 2013-12-29 | Suspension brain sheet antigen retrieval piece pressing clip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310736656.5A CN103712837B (en) | 2013-12-29 | 2013-12-29 | Suspension brain sheet antigen retrieval piece pressing clip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103712837A true CN103712837A (en) | 2014-04-09 |
| CN103712837B CN103712837B (en) | 2015-10-28 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201310736656.5A Expired - Fee Related CN103712837B (en) | 2013-12-29 | 2013-12-29 | Suspension brain sheet antigen retrieval piece pressing clip |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105751101A (en) * | 2016-03-23 | 2016-07-13 | 宁波江丰生物信息技术有限公司 | Slice fixing device for preventing slice from falling |
| CN106979884A (en) * | 2017-04-20 | 2017-07-25 | 王新宇 | A kind of Urine Analyzer for being easy to shake up reagent |
| CN108931412A (en) * | 2017-05-26 | 2018-12-04 | 深圳大学 | Histotomy antigen retrieval device and method |
Citations (9)
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| US5244787A (en) * | 1991-01-31 | 1993-09-14 | Biogenex Laboratories | Antigen retrieval in formalin-fixed tissues using microwave energy |
| CN2305995Y (en) * | 1997-10-17 | 1999-02-03 | 刘政国 | Antigen repairing box for microwave oven |
| CN1737527A (en) * | 2005-09-07 | 2006-02-22 | 杭州电子科技大学 | A kind of rapid installing section and thermostat |
| CN201382939Y (en) * | 2009-03-20 | 2010-01-13 | 无锡博慧斯生物医药科技有限公司 | Novel immunohistochemical experimental apparatus |
| CN201942685U (en) * | 2011-01-14 | 2011-08-24 | 刘超男 | Repair box suitable for repairing tissue antigen by microwave method |
| US20120009666A1 (en) * | 2009-08-26 | 2012-01-12 | General Electric Company | Method and apparatus for antigen retrieval process |
| CN202511991U (en) * | 2012-04-10 | 2012-10-31 | 吉林金域医学检验所有限公司 | Pathological section frame convenient to move |
| CN202710381U (en) * | 2012-08-24 | 2013-01-30 | 佳木斯大学 | Anti-dropping slide device |
| CN203672691U (en) * | 2013-12-29 | 2014-06-25 | 中国人民解放军成都军区总医院 | Pressure plate type antigen retrieval frame for suspended brain slice |
-
2013
- 2013-12-29 CN CN201310736656.5A patent/CN103712837B/en not_active Expired - Fee Related
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5244787A (en) * | 1991-01-31 | 1993-09-14 | Biogenex Laboratories | Antigen retrieval in formalin-fixed tissues using microwave energy |
| CN2305995Y (en) * | 1997-10-17 | 1999-02-03 | 刘政国 | Antigen repairing box for microwave oven |
| CN1737527A (en) * | 2005-09-07 | 2006-02-22 | 杭州电子科技大学 | A kind of rapid installing section and thermostat |
| CN201382939Y (en) * | 2009-03-20 | 2010-01-13 | 无锡博慧斯生物医药科技有限公司 | Novel immunohistochemical experimental apparatus |
| US20120009666A1 (en) * | 2009-08-26 | 2012-01-12 | General Electric Company | Method and apparatus for antigen retrieval process |
| CN201942685U (en) * | 2011-01-14 | 2011-08-24 | 刘超男 | Repair box suitable for repairing tissue antigen by microwave method |
| CN202511991U (en) * | 2012-04-10 | 2012-10-31 | 吉林金域医学检验所有限公司 | Pathological section frame convenient to move |
| CN202710381U (en) * | 2012-08-24 | 2013-01-30 | 佳木斯大学 | Anti-dropping slide device |
| CN203672691U (en) * | 2013-12-29 | 2014-06-25 | 中国人民解放军成都军区总医院 | Pressure plate type antigen retrieval frame for suspended brain slice |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105751101A (en) * | 2016-03-23 | 2016-07-13 | 宁波江丰生物信息技术有限公司 | Slice fixing device for preventing slice from falling |
| CN106979884A (en) * | 2017-04-20 | 2017-07-25 | 王新宇 | A kind of Urine Analyzer for being easy to shake up reagent |
| CN108931412A (en) * | 2017-05-26 | 2018-12-04 | 深圳大学 | Histotomy antigen retrieval device and method |
| CN108931412B (en) * | 2017-05-26 | 2020-12-04 | 深圳大学 | Tissue slice antigen retrieval device and method |
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| Publication number | Publication date |
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| CN103712837B (en) | 2015-10-28 |
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