CN104082275B - A form of fish embryos microscopic slide specimens of animal production technology - Google Patents

A form of fish embryos microscopic slide specimens of animal production technology Download PDF

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Publication number
CN104082275B
CN104082275B CN 201410308564 CN201410308564A CN104082275B CN 104082275 B CN104082275 B CN 104082275B CN 201410308564 CN201410308564 CN 201410308564 CN 201410308564 A CN201410308564 A CN 201410308564A CN 104082275 B CN104082275 B CN 104082275B
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CN 201410308564
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CN104082275A (en )
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郭恩棉
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青岛农业大学
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Abstract

本发明提供了一种简单有效的水产动物胚胎显微玻片标本制作技术,在实现水产动物胚胎长期保存的同时,也实现了最大限度保存材料的形态结构,并提高标本观察的清晰度。 The present invention provides a simple and effective microscopic slide preparations aquatic animal embryo production technology, while achieving long-term preservation of aquatic animal embryos, but also to achieve the maximum storage material morphology, and to improve the clarity of the specimen was observed. 本发明以凹玻片为载体,采用固定、脱水、透明、封片等技术方案,制作水产动物胚胎显微玻片标本,操作过程中以福尔马林为固定剂、酒精为脱水剂、甘油为透明剂、中性树胶为封片剂。 The present invention in a carrier recess glass slides, fixed, dehydrated, cleared, and the like were mounted aspect, aquatic animal embryo produced microscopic specimen slide, during operation as a formalin fixative, dehydrated alcohol, glycerin transparent agent, is sealed with neutral gum tablet.

Description

一种水产动物胚胎显微玻片标本制作技术 A form of fish embryos microscopic slide specimens of animal production technology

技术领域 FIELD

[0001] 本发明涉及一种水产动物胚胎显微标本制作技术,涉及显微镜标本制作领域,具体地说是一种水产动物胚胎显微液基凹玻片标本制作技术。 [0001] The present invention relates to an aquatic animal embryo microscopy specimen preparation techniques, to the field of microscopy specimen preparation, particularly aquatic animal embryo is a microscopic specimen slide recess liquid-based production techniques.

背景技术 Background technique

[0002]目前永久性显微整体保存标本一般都是把材料经过固定、干燥、封固等步骤制作,使得材料失去了生活状态下的一些特殊结构;并且有些体积较大的材料不能通过上述步骤制作整体保存材料,通常只能取新鲜样品观察或用固定液固定,需要时取出观察,这不利于材料的长期保存,或在材料多次取用过程中造成材料损坏。 [0002] It is generally permanently preserved specimens are whole microstructure material through the fixing, drying, sealing and other production steps, so that the material loses some special structure in the living state; and somewhat larger volume of material by the above steps is not create an overall saving material, usually only take a fresh sample observation or fixing fixative, take it out when needed, which is not conducive to long-term preservation of the material, or damage to the material after multiple access process. 一种液基标本制作玻片的发明(申请号:201320491346.7)使体积稍大的材料制成显微整体玻片标本成为可能。 Is made slightly larger than the volume of the material microstructure integrally slide specimens possible: one kind of liquid-based specimen preparation of the present invention slides (Application No. 201320491346.7).

发明内容 SUMMARY

[0003] 针对现有技术的不足,本发明的目的是提供一种简单有效的水产动物胚胎显微玻片液基标本制作技术,以实现水产动物胚胎的长期保存,同时也实现最大限度的保存材料形态结构,并提高标本观察的清晰度。 [0003] for the deficiencies of the prior art, an object of the present invention to provide a simple and effective solution aquatic animal embryo microscopic slide yl specimen preparation techniques, aquatic animal embryo to achieve long-term storage, but also to achieve the maximum preservation material morphology, and to improve the clarity of the samples was observed.

[0004] 为了解决上述技术问题,本发明采用了固定、脱水、透明、封片等技术方案,具体包含以下步骤: [0004] To solve the above problems, the present invention employs a fixed, dehydrated, cleared, and the like were mounted technical solution, particularly comprising the steps of:

[0005] I)采取水产动物胚胎材料进行固定,固定液为5〜10%的福尔马林; [0005] I) taking embryos aquatic animal material is fixed, the fixing solution is 5 to 10% formalin;

[0006] 2)以梯度浓度酒精为脱水剂对固定好的水产动物胚胎材料进行脱水; [0006] 2) a concentration gradient dehydrated alcohol as a dehydrating agent to the fixing material good aquatic animal embryo;

[0007] 3)以甘油为透明剂对脱好水的水产动物胚胎材料进行透明; [0007] 3) good water removal from glycerol of aquatic animal embryos transparent material is a transparent agent;

[0008] 4)吸取适量甘油和透明好的水产动物胚胎材料,加入液基标本制作凹玻片; [0008] 4) and the suction amount of glycerol good aquatic animal embryo transparent material, the liquid-based specimen preparation was added concave slide;

[0009] 5)以中性树胶为封片剂对玻片进行封片,干燥后即可观察和保存。 [0009] 5) as the neutral gum tablet slides were blocked were mounted, can be observed after drying and storage.

[0010] 本发明的水产动物胚胎显微标本制作技术与现有技术相比,所产生的有益效果是: [0010] Aquatic animal embryo microscopy specimen preparation techniques of the present invention and the prior art comparison, the beneficial effects are produced:

[0011] I)本发明以凹玻片为载体,将处理好的标本,一般来说是透明性较好的标本直接加入凹玻片凹陷区域,封片保存即可,操作方便简洁,易于操作; [0011] I) of the present invention to recess the slide as the carrier, good processing specimens, with good transparency is generally added directly to the specimen slide concave recessed region, were mounted to save, simple and easy to operate, easy to operate ;

[0012] 2)应用本发明的方法,实现了水产动物胚胎长期保存显微整体玻片标本的制作,方便材料的保存和观察; [0012] 2) application of the method according to the present invention, the aquatic animal embryo to achieve long-term preservation of microscopic specimen slide overall production, storage and easy observation of the material;

[0013] 3)应用本发明的方法,同时也实现了最大限度的保存水产动物胚胎的形态结构,并提高了标本观察的清晰度。 [0013] 3) the method of the invention, but also to achieve the maximum preservation of aquatic animal embryo morphology, and to improve the clarity of the samples was observed.

具体实施方式 detailed description

[0014] 上述水产动物胚胎显微玻片标本制作技术是按照以下步骤进行的: [0014] The microscopic slide preparations aquatic animal embryo production techniques are carried out according to the following steps:

[0015] I)采取水产动物胚胎材料,用5〜10%的福尔马林固定12〜24小时; [0015] I) taking embryo material aquatic animals, with 5 to 10% formalin-fixed 12~24 hours;

[0016] 2)把以上固定好的水产动物胚胎材料,依次经过蒸馏水、70%酒精、80%酒精、95%酒精和两步无水酒精进彳丁脱水,每级药品各历时30分钟左右; [0016] 2) The above material secured good aquatic animal embryos, distilled water sequentially through 70% ethanol, 80% ethanol, 95% ethanol and left foot into two steps butoxy ethanol dehydration, per each class of drugs over about 30 minutes;

[0017] 3)把以上脱好水的水产动物胚胎材料,依次经过体积比为1:1的甘油和无水酒精混合液、两步纯甘油进行透明,每级药品各历时30分钟左右; [0017] 3) The above de good water aquatic animal embryo material, passes through the volume ratio of 1: 1 mixture of glycerin and ethanol, the pure glycerol transparent two-step, each of the drug over each stage of about 30 minutes;

[0018] 4)吸取甘油和透明好的水产动物胚胎材料,加入液基标本制作凹玻片,加入的量以不溢出凹玻片凹槽边界为限; [0018] 4) good suction glycerol and aquatic animal embryos transparent material, the liquid-based specimen preparation was added concave slides, added in an amount so as not to overflow the recessed slide groove boundary limit;

[0019] 5)以中性树胶为封片剂对玻片进行封片,干燥后即可观察和保存。 [0019] 5) as the neutral gum tablet slides were blocked were mounted, can be observed after drying and storage.

[0020] 以上是对本发明的较佳实施进行了具体说明,但本发明创造并不限于所述实施 [0020] The foregoing is the preferred embodiment of the present invention have been specifically described, but the present invention is not limited to the creation of embodiments

[0021]例,熟悉本领域的技术人员在不违背本发明精神的前提下还可做作出种种的等同变形或替 [0021] embodiment, those skilled in the art without departing from the spirit of the present invention may also be modified to make various equivalent or alternative

[0022] 换,这些等同的变形或替换均包含在本申请权利要求所限定的范围内。 [0022] In other such equivalent modifications or substitutions are included in the present application within the scope defined by the claims.

Claims (1)

  1. 1.一种水产动物胚胎凹玻片液基显微标本制作方法,采用固定、脱水、透明、封片技术方案,其步骤在于: (1)采取水产动物胚胎材料,用5〜10%的福尔马林固定12〜24小时; (2)把以上固定好的水产动物胚胎材料,依次经过蒸馏水、70%酒精、80%酒精、95%酒精和两步无水酒精进行脱水,每级药品各历时30分钟; (3)把以上脱好水的水产动物胚胎材料,依次经过体积比为1:1的甘油和无水酒精混合液、两步纯甘油进行透明,每级药品各历时30分钟; (4)吸取甘油和透明好的水产动物胚胎材料,加入液基标本制作凹玻片,加入的量以不溢出凹玻片凹槽边界为限,以中性树胶为封片剂对玻片进行封片,干燥后即可观察和保存。 An aquatic animal embryo liquid-based slide recess microscopy specimen preparation method, fixed, dehydrated, cleared Fengpian aspect, the steps comprising: (1) taking embryo material aquatic animals, with 5 to 10% of blessing formalin fixed 12~24 hours; (2) the above material secured good aquatic animal embryos, distilled water sequentially through 70% ethanol, 80% ethanol, 95% ethanol, and dehydrated ethanol in two steps, each level the respective drugs for 30 min; (3) good water removal than the aquatic animal embryo material, passes through the volume ratio of 1: 1 mixture of glycerin and ethanol, the pure glycerol transparent two steps, each level of each drugs for 30 min; (4) good suction glycerol and aquatic animal embryo transparent material, the liquid-based specimen preparation was added concave slides, added in an amount so as not to overflow the recessed slide groove boundary is limited to neutral gum tablet slides were sealed as It was mounted to observe and preservation after drying.
CN 201410308564 2014-07-02 2014-07-02 A form of fish embryos microscopic slide specimens of animal production technology CN104082275B (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005117962A1 (en) * 2004-06-02 2005-12-15 Victor Bronshtein Preservation by vaporization
CN1961816A (en) * 2006-11-24 2007-05-16 山东建筑大学 Method for making fetus transparent specimen
CN101790975A (en) * 2010-03-26 2010-08-04 山西大学 Manufacturing method of daphnia microslide specimen
CN102640742A (en) * 2012-04-20 2012-08-22 杭州市农业科学研究院 Improved manufacturing method for plant wireworm permanent slide
CN103868774A (en) * 2014-03-26 2014-06-18 青岛大学 Method for manufacturing parasitic ovum permanent slide specimens

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005117962A1 (en) * 2004-06-02 2005-12-15 Victor Bronshtein Preservation by vaporization
CN1961816A (en) * 2006-11-24 2007-05-16 山东建筑大学 Method for making fetus transparent specimen
CN101790975A (en) * 2010-03-26 2010-08-04 山西大学 Manufacturing method of daphnia microslide specimen
CN102640742A (en) * 2012-04-20 2012-08-22 杭州市农业科学研究院 Improved manufacturing method for plant wireworm permanent slide
CN103868774A (en) * 2014-03-26 2014-06-18 青岛大学 Method for manufacturing parasitic ovum permanent slide specimens

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