CN104082275B - A kind of aquatic livestock embryo microscope slide sample manufacturing technology - Google Patents
A kind of aquatic livestock embryo microscope slide sample manufacturing technology Download PDFInfo
- Publication number
- CN104082275B CN104082275B CN201410308564.1A CN201410308564A CN104082275B CN 104082275 B CN104082275 B CN 104082275B CN 201410308564 A CN201410308564 A CN 201410308564A CN 104082275 B CN104082275 B CN 104082275B
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- CN
- China
- Prior art keywords
- aquatic livestock
- alcohol
- transparent
- embryo
- fetal material
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- 244000144972 livestock Species 0.000 title claims abstract description 26
- 210000001161 mammalian embryo Anatomy 0.000 title claims abstract description 14
- 238000005516 engineering process Methods 0.000 title abstract description 10
- 238000004519 manufacturing process Methods 0.000 title abstract description 7
- 239000000463 material Substances 0.000 claims abstract description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 12
- 235000011187 glycerol Nutrition 0.000 claims abstract description 9
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 8
- 230000007935 neutral effect Effects 0.000 claims abstract description 4
- 230000018044 dehydration Effects 0.000 claims abstract description 3
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 3
- 230000001605 fetal effect Effects 0.000 claims description 12
- 238000004321 preservation Methods 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000003814 drug Substances 0.000 claims description 4
- 150000001298 alcohols Chemical class 0.000 claims description 2
- 239000012153 distilled water Substances 0.000 claims description 2
- 150000002314 glycerols Chemical class 0.000 claims description 2
- 238000005464 sample preparation method Methods 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 4
- 239000012024 dehydrating agents Substances 0.000 abstract description 2
- 239000000834 fixative Substances 0.000 abstract 1
- 230000007774 longterm Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a kind of simple and effective aquatic livestock embryo microscope slide sample manufacturing technology, realizing while aquatic livestock embryo preserves for a long time, also achieving the morphosis preserving material to greatest extent, and improve the definition that sample observes.The present invention take concave slide as carrier, adopt the technical schemes such as fixing, dehydration, transparent, mounting, making aquatic livestock embryo microscope slide sample is fixative with formalin in operating process, alcohol is dehydrating agent, glycerine is clarifier, neutral gum is mountant.
Description
Technical field
The present invention relates to a kind of aquatic livestock embryo microscopic sample manufacturing technology, relate to microscope specimen and make field, specifically a kind of aquatic livestock embryo micro-Ye Ji concave slide preparation of specimen technology.
Background technology
It is all generally that material is made through the step such as fixing, drying, sealing that current permanent micro-entirety preserves sample, some special constructions under making material lose animation; And the material that some volume is larger does not make overall preservation material by above-mentioned steps, usually can only get fresh sample observe or fix with fixer, take out when needing and observe, this is unfavorable for the long-term preservation of material, or repeatedly takes in process at material and cause material damage.A kind of liquid-based specimens makes the invention (application number: 201320491346.7) making the slightly large material of volume make micro-overall slide sample becomes possibility of slide.
Summary of the invention
For the deficiencies in the prior art, the object of this invention is to provide a kind of simple and effective aquatic livestock embryo microscope slide liquid-based specimens manufacturing technology, to realize the long-term preservation of aquatic livestock embryo, also realize preserving material shape structure to greatest extent simultaneously, and improve the definition of sample observation.
In order to solve the problems of the technologies described above, present invention employs the technical schemes such as fixing, dehydration, transparent, mounting, specifically comprising following steps:
1) take aquatic livestock fetal material to be fixed, fixer is the formalin of 5 ~ 10%;
2) with gradient concentration alcohol for dehydrating agent dewaters to the aquatic livestock fetal material fixed;
3) be that the aquatic livestock fetal material of clarifier to de-good water carries out transparent with glycerine;
4) draw qs glycerin and transparent good aquatic livestock fetal material, add liquid-based specimens and make concave slide;
5) be that mountant carries out mounting to slide with neutral gum, i.e. observable and preservation after dry.
Compared with prior art, the beneficial effect produced is aquatic livestock embryo microscopic sample manufacturing technology of the present invention:
1) the present invention take concave slide as carrier, and by the sample handled well, be in general that the good sample of the transparency directly adds concave slide sunk area, mounting is preserved, succinctly easy to operate, is easy to operation;
2) apply method of the present invention, achieve the making that aquatic livestock embryo preserves micro-overall slide sample for a long time, facilitate preservation and the observation of material;
3) apply method of the present invention, also achieve the morphosis preserving aquatic livestock embryo to greatest extent simultaneously, and improve the definition of sample observation.
Embodiment
Above-mentioned aquatic livestock embryo microscope slide sample manufacturing technology is carried out according to following steps:
1) take aquatic livestock fetal material, the formalin with 5 ~ 10% fixes 12 ~ 24 hours;
2) the aquatic livestock fetal material fixed above, dewater through distilled water, 70% alcohol, 80% alcohol, 95% alcohol and two step absolute alcohols successively, every grade of medicine lasts about 30 minutes;
3) the aquatic livestock fetal material of above de-good water, be that the glycerine of 1:1 and absolute alcohol mixed liquor, two step pure glycerins carry out transparent through volume ratio successively, every grade of medicine lasts about 30 minutes;
4) draw glycerine and transparent good aquatic livestock fetal material, add liquid-based specimens and make concave slide, the amount added is limited not overflow concave slide groove border;
5) be that mountant carries out mounting to slide with neutral gum, i.e. observable and preservation after dry.
Be more than that better enforcement of the present invention is illustrated, but the invention is not limited to described enforcement
Example, those of ordinary skill in the art also can make all equivalent variations or replace under the prerequisite without prejudice to spirit of the present invention
Change, these equivalent distortion or replacement are all included in the application's claim limited range.
Claims (1)
1. an aquatic livestock embryo concave slide liquid base microscopic sample preparation method, adopt fixing, dehydration, transparent, mounting technical scheme, its step is:
(1) take aquatic livestock fetal material, the formalin with 5 ~ 10% fixes 12 ~ 24 hours;
(2) the aquatic livestock fetal material fixed above, dewater through distilled water, 70% alcohol, 80% alcohol, 95% alcohol and two step absolute alcohols successively, every grade of medicine lasts 30 minutes;
(3) the aquatic livestock fetal material of above de-good water, be that the glycerine of 1:1 and absolute alcohol mixed liquor, two step pure glycerins carry out transparent through volume ratio successively, every grade of medicine lasts 30 minutes;
(4) draw glycerine and transparent good aquatic livestock fetal material, add liquid-based specimens and make concave slide, the amount added is limited not overflow concave slide groove border, is that mountant carries out mounting to slide with neutral gum, i.e. observable and preservation after dry.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410308564.1A CN104082275B (en) | 2014-07-02 | 2014-07-02 | A kind of aquatic livestock embryo microscope slide sample manufacturing technology |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410308564.1A CN104082275B (en) | 2014-07-02 | 2014-07-02 | A kind of aquatic livestock embryo microscope slide sample manufacturing technology |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104082275A CN104082275A (en) | 2014-10-08 |
| CN104082275B true CN104082275B (en) | 2016-03-23 |
Family
ID=51630127
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410308564.1A Expired - Fee Related CN104082275B (en) | 2014-07-02 | 2014-07-02 | A kind of aquatic livestock embryo microscope slide sample manufacturing technology |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104082275B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116491495B (en) * | 2023-06-26 | 2023-09-19 | 中国海洋大学 | Equipment for manufacturing whole chip-loading specimen of concave slide shellfish larva |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005117962A1 (en) * | 2004-06-02 | 2005-12-15 | Victor Bronshtein | Preservation by vaporization |
| CN1961816A (en) * | 2006-11-24 | 2007-05-16 | 山东建筑大学 | Method for making fetus transparent specimen |
| CN101790975A (en) * | 2010-03-26 | 2010-08-04 | 山西大学 | Manufacturing method of daphnia microslide specimen |
| CN102640742A (en) * | 2012-04-20 | 2012-08-22 | 杭州市农业科学研究院 | Improved manufacturing method for plant wireworm permanent slide |
| CN103868774A (en) * | 2014-03-26 | 2014-06-18 | 青岛大学 | Method for manufacturing parasitic ovum permanent slide specimens |
-
2014
- 2014-07-02 CN CN201410308564.1A patent/CN104082275B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2005117962A1 (en) * | 2004-06-02 | 2005-12-15 | Victor Bronshtein | Preservation by vaporization |
| CN1961816A (en) * | 2006-11-24 | 2007-05-16 | 山东建筑大学 | Method for making fetus transparent specimen |
| CN101790975A (en) * | 2010-03-26 | 2010-08-04 | 山西大学 | Manufacturing method of daphnia microslide specimen |
| CN102640742A (en) * | 2012-04-20 | 2012-08-22 | 杭州市农业科学研究院 | Improved manufacturing method for plant wireworm permanent slide |
| CN103868774A (en) * | 2014-03-26 | 2014-06-18 | 青岛大学 | Method for manufacturing parasitic ovum permanent slide specimens |
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| Publication number | Publication date |
|---|---|
| CN104082275A (en) | 2014-10-08 |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160323 |
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