CN103868774A - Method for manufacturing parasitic ovum permanent slide specimens - Google Patents
Method for manufacturing parasitic ovum permanent slide specimens Download PDFInfo
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- CN103868774A CN103868774A CN201410115789.5A CN201410115789A CN103868774A CN 103868774 A CN103868774 A CN 103868774A CN 201410115789 A CN201410115789 A CN 201410115789A CN 103868774 A CN103868774 A CN 103868774A
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Abstract
The invention belongs to the field of human parasitology of medical science and provides a method for manufacturing parasitic ovum permanent slide specimens. The method comprises the following steps: cleaning and fixing parasitic ova, performing centrifugal precipitation, performing freeze drying, performing infiltration in neutral balsam and mounting. According to the method, valuable parasitic ova can be made into slide specimens for permanent preservation, and waste of parasitic ova (particularly rare ova) is avoided. Particularly, the teaching slide specimens can be repeatedly used for a long time in the field of teaching and scientific research, and lots of manpower, financial resources and material resources are saved.
Description
Technical field
The invention belongs to medical parasitology field, relate to a kind of parasitic ovum permanent slide Slide processing, can be used for making various parasitic ovum permanent slide samples, especially can in Human Parasitology Teaching and scientific research, use.
Background technology
Parasitic ovum is the Main Diagnosis stage of parasitic disease, is also important experiment material in parasitology teaching and scientific research process.At present, parasitic ovum slide sample is divided into interim smear preparation and long-term slide sample, and interim smear preparation can only disposablely use, and long-term slide sample also can only be preserved 2 ~ 3 years.Due to this class sample, to preserve service time short, need to waste a large amount of worm's ovums and constantly make supplementaryly, and therefore, in parasitology teaching and scientific research, the parasitic ovum that especially some difficulties collect, more needs to be made into permanent slide sample for teaching and scientific research for a long time.
Summary of the invention
The object of this invention is to provide a kind of parasitic ovum permanent slide Slide processing, the worm's ovum sample of making can use for a long time and preserve.
Parasitic ovum permanent slide Slide processing provided by the invention, follows these steps to carry out:
A, worm's ovum clean fixing: the worm's ovum of collection is put into centrifuge tube, add 30% ~ 50% alcohol of two volumes, concussion mixes, and places 6 ~ 12 hours;
B, centrifugation: centrifuge tube is put into hydro-extractor, 1500 ~ 3000 revs/min, centrifugal 3 ~ 8 minutes, abandon supernatant, leave and take sediment;
C, freeze drying: sediment is put into vacuum freeze drier, 12 ~ 24 hours;
D, neutral gum infiltrate: by neutral gum and dimethylbenzene 1:1 mixing by volume, join in dry sediment, stir and evenly mix and make it to become worm egg suspension, adjusting worm's ovum concentration in suspending liquid is 500 ~ 1500/ml, places 1 ~ 2 hour;
E, mounting: stir and evenly mix worm egg suspension, draw a trichomonad ovum hanging drop to microslide with suction pipe, covered, dries, and makes worm's ovum permanent slide sample.
The invention has the beneficial effects as follows, created the new method of parasitic ovum permanent slide preparation of specimen.Valuable worm's ovum can be made to slide sample persistence by the method, avoid worm's ovum (particularly rare worm's ovum) waste; Especially in teaching and scientific research field, the slide sample that can make to impart knowledge to students is reused for a long time, saves a large amount of manpowers, financial resources and material resources.
Embodiment:
Embodiment 1
Diplacanthus nanus ovum permanent slide preparation of specimen, step is as follows:
A, worm's ovum clean fixing: the Diplacanthus nanus ovum of collection is put into centrifuge tube, add 40% alcohol of two volumes, concussion mixes, and places 8 hours;
B, centrifugation: centrifuge tube is put into hydro-extractor, 2500 revs/min, centrifugal 5 minutes, abandon supernatant, leave and take sediment;
C, freeze drying: sediment is put into vacuum freeze drier, 15 hours;
D, neutral gum infiltrate: by neutral gum and dimethylbenzene 1:1 mixing by volume, join in dry sediment, stir and evenly mix and make it to become worm egg suspension, adjusting worm's ovum concentration in suspending liquid is 800/ml, places 1 hour;
E, mounting: stir and evenly mix worm egg suspension, draw a trichomonad ovum hanging drop to microslide with suction pipe, covered, dries, and makes Diplacanthus nanus ovum permanent slide sample.
Embodiment 2
Paragonismus westermani ovum permanent slide preparation of specimen, step is as follows:
A, worm's ovum clean fixing: the Paragonismus westermani ovum of collection is put into centrifuge tube, add 50% alcohol of two volumes, concussion mixes, and places 10 hours;
B, centrifugation: centrifuge tube is put into hydro-extractor, 2000 revs/min, centrifugal 6 minutes, abandon supernatant, leave and take sediment;
C, freeze drying: sediment is put into vacuum freeze drier, 18 hours;
D, neutral gum infiltrate: by neutral gum and dimethylbenzene 1:1 mixing by volume, join in dry sediment, stir and evenly mix and make it to become worm egg suspension, adjusting worm's ovum concentration in suspending liquid is 600/ml, places 2 hours;
E, mounting: stir and evenly mix worm egg suspension, draw a trichomonad ovum hanging drop to microslide with suction pipe, covered, dries, and makes Paragonismus westermani ovum permanent slide sample.
Embodiment 3
Unfertilized roundworm egg permanent slide preparation of specimen, step is as follows:
A, worm's ovum clean fixing: the unfertilized roundworm egg of collecting is put into centrifuge tube, add 50% alcohol of two volumes, concussion mixes, and places 12 hours;
B, centrifugation: centrifuge tube is put into hydro-extractor, 1800 revs/min, centrifugal 5 minutes, abandon supernatant, leave and take sediment;
C, freeze drying: sediment is put into vacuum freeze drier, 20 hours;
D, neutral gum infiltrate: by neutral gum and dimethylbenzene 1:1 mixing by volume, join in dry sediment, stir and evenly mix and make it to become worm egg suspension, adjusting worm's ovum concentration in suspending liquid is 1000/ml, places 1.5 hours;
E, mounting: stir and evenly mix worm egg suspension, draw a trichomonad ovum hanging drop to microslide with suction pipe, covered, dries, and makes unfertilized roundworm egg permanent slide sample.
Claims (1)
1. a parasitic ovum permanent slide Slide processing, is characterized in that:
A, worm's ovum clean fixing: the worm's ovum of collection is put into centrifuge tube, add 30% ~ 50% alcohol of two volumes, concussion mixes, and places 6 ~ 12 hours;
B, centrifugation: centrifuge tube is put into hydro-extractor, 1500 ~ 3000 revs/min, centrifugal 3 ~ 8 minutes, abandon supernatant, leave and take sediment;
C, freeze drying: sediment is put into vacuum freeze drier, 12 ~ 24 hours;
D, neutral gum infiltrate: by neutral gum and dimethylbenzene 1:1 mixing by volume, join in dry sediment, stir and evenly mix and make it to become worm egg suspension, adjusting worm's ovum concentration in suspending liquid is 500 ~ 1500/ml, places 1 ~ 2 hour;
E, mounting: stir and evenly mix worm egg suspension, draw a trichomonad ovum hanging drop to microslide with suction pipe, covered, dries, and makes worm's ovum permanent slide sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115789.5A CN103868774A (en) | 2014-03-26 | 2014-03-26 | Method for manufacturing parasitic ovum permanent slide specimens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410115789.5A CN103868774A (en) | 2014-03-26 | 2014-03-26 | Method for manufacturing parasitic ovum permanent slide specimens |
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| CN103868774A true CN103868774A (en) | 2014-06-18 |
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| CN201410115789.5A Pending CN103868774A (en) | 2014-03-26 | 2014-03-26 | Method for manufacturing parasitic ovum permanent slide specimens |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082275A (en) * | 2014-07-02 | 2014-10-08 | 青岛农业大学 | Preparation technology of aquatic animal embryo microscope slide specimen |
| CN104142264A (en) * | 2014-08-08 | 2014-11-12 | 青岛大学 | Method for making long-term-used parasitic ovum slide specimen |
| CN106614520A (en) * | 2016-11-29 | 2017-05-10 | 宁波检验检疫科学技术研究院 | Manufacturing method of plant nematode permanent slide |
| CN107798981A (en) * | 2017-10-31 | 2018-03-13 | 楚雄医药高等专科学校 | A kind of permanent teaching micro-capsule sample slice and preparation method thereof |
| CN110243648A (en) * | 2019-07-11 | 2019-09-17 | 四川省疾病预防控制中心 | A method of schistosome ovum sample is made using improvement plus rattan thick smear |
| CN112326381A (en) * | 2020-10-27 | 2021-02-05 | 南京大恩美生物技术有限公司 | Method for preparing egg slide specimen suitable for intestinal parasites of human body and animals |
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| CN1059445A (en) * | 1990-09-03 | 1992-03-18 | 赖群 | Three generics of mosquito each phase teaching slide sample new method history of life |
| CN101790975A (en) * | 2010-03-26 | 2010-08-04 | 山西大学 | Manufacturing method of daphnia microslide specimen |
| CN103698184A (en) * | 2013-12-28 | 2014-04-02 | 青岛大学 | Device for manufacturing parasite egg slide specimen for long-term use |
-
2014
- 2014-03-26 CN CN201410115789.5A patent/CN103868774A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1059445A (en) * | 1990-09-03 | 1992-03-18 | 赖群 | Three generics of mosquito each phase teaching slide sample new method history of life |
| CN101790975A (en) * | 2010-03-26 | 2010-08-04 | 山西大学 | Manufacturing method of daphnia microslide specimen |
| CN103698184A (en) * | 2013-12-28 | 2014-04-02 | 青岛大学 | Device for manufacturing parasite egg slide specimen for long-term use |
Non-Patent Citations (6)
| Title |
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| 佘俊萍等: "寄生虫卵标本制作改进及使用效果", 《泸州医学院学报》 * |
| 宋军科等: "牛羊消化道线虫虫卵永久性玻片制作方法", 《西北农业学报》 * |
| 常正山: "寄生虫标本的采集和保存", 《中国寄生虫学与寄生虫病杂志》 * |
| 林丽卿: "寄生虫虫卵永久性玻片标本的制备", 《实用医技杂志》 * |
| 胡守锋等: "永久性混合虫卵玻片标本制作和使用效果观察", 《中国病原生物学杂志》 * |
| 陈熙等: "三种永久虫卵封片标本制作方法的比较", 《昆明医科大学学报》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104082275A (en) * | 2014-07-02 | 2014-10-08 | 青岛农业大学 | Preparation technology of aquatic animal embryo microscope slide specimen |
| CN104082275B (en) * | 2014-07-02 | 2016-03-23 | 青岛农业大学 | A kind of aquatic livestock embryo microscope slide sample manufacturing technology |
| CN104142264A (en) * | 2014-08-08 | 2014-11-12 | 青岛大学 | Method for making long-term-used parasitic ovum slide specimen |
| CN106614520A (en) * | 2016-11-29 | 2017-05-10 | 宁波检验检疫科学技术研究院 | Manufacturing method of plant nematode permanent slide |
| CN107798981A (en) * | 2017-10-31 | 2018-03-13 | 楚雄医药高等专科学校 | A kind of permanent teaching micro-capsule sample slice and preparation method thereof |
| CN110243648A (en) * | 2019-07-11 | 2019-09-17 | 四川省疾病预防控制中心 | A method of schistosome ovum sample is made using improvement plus rattan thick smear |
| CN110243648B (en) * | 2019-07-11 | 2020-06-02 | 四川省疾病预防控制中心 | Method for preparing schistosome ovum specimen by adopting improved rattan-added thick smear |
| CN112326381A (en) * | 2020-10-27 | 2021-02-05 | 南京大恩美生物技术有限公司 | Method for preparing egg slide specimen suitable for intestinal parasites of human body and animals |
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Application publication date: 20140618 |