CN107798981A - A kind of permanent teaching micro-capsule sample slice and preparation method thereof - Google Patents
A kind of permanent teaching micro-capsule sample slice and preparation method thereof Download PDFInfo
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- CN107798981A CN107798981A CN201711043095.5A CN201711043095A CN107798981A CN 107798981 A CN107798981 A CN 107798981A CN 201711043095 A CN201711043095 A CN 201711043095A CN 107798981 A CN107798981 A CN 107798981A
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- sample slice
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- G—PHYSICS
- G09—EDUCATION; CRYPTOGRAPHY; DISPLAY; ADVERTISING; SEALS
- G09B—EDUCATIONAL OR DEMONSTRATION APPLIANCES; APPLIANCES FOR TEACHING, OR COMMUNICATING WITH, THE BLIND, DEAF OR MUTE; MODELS; PLANETARIA; GLOBES; MAPS; DIAGRAMS
- G09B23/00—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes
- G09B23/28—Models for scientific, medical, or mathematical purposes, e.g. full-sized devices for demonstration purposes for medicine
Abstract
The invention discloses a kind of permanent teaching micro-capsule sample slice and preparation method thereof, described permanent teaching includes micro-capsule sample slice body and transparent vacuum container with micro-capsule sample slice, described micro-capsule sample slice body includes slide, first cover glass, second cover glass, microcapsule layer, band, described microcapsule layer is arranged on slide, and the top of microcapsule layer covers the first cover glass, bonded between described the first cover glass and the second cover glass by neutral gum, described band includes being arranged on the band that the second cover glass surrounding is used to seal between the second cover glass and slide, described micro-capsule sample slice body is placed in transparent vacuum container.The simple science of preparation method of the present invention, the permanent teaching micro-capsule sample slice of preparation, observation shape is completely clear under the microscope, effectively increases the intuitive of teaching, improves the quality of teaching, while simple in construction, can preserve for a long time.
Description
Technical field
The invention belongs to medical teaching mode manufacture technology field, and in particular to micro-capsule sample slice is used in a kind of permanent teaching
And preparation method thereof.
Background technology
Microencapsulation（Abbreviation micro-capsule）It is a kind of novel form to grow up the 1960s.Medicine preparation is into after micro-capsule
With cover medicine bad smell, increase medicine stability, reduce volatile ingredient loss, make liquid medicine solidification,
The advantages that rate of release of medicine can effectively be controlled, especially in recent years be used for cancer therapy drug preparation, can make drug accumulation in
The target area such as liver or lung, the effect of improving medicine, reduce its toxic side effect.So in the pharmacy and relevant speciality medicine of medical college
In agent course, micro-capsule is the knowledge point that student must be learned by and grasp, but because this formulation is a kind of microscopic, spherical capsule
Body（Diameter is at 1 ~ 250 μm）, including medicine（Capsule-core）With capsule material two parts of packaging medicine, only can just see under the microscope
Understand its structure, if can refer to without material object in experimental teaching, it is micro- that student can not judge whether self-made product forms
Capsule.But at present, problems also be present in the making and preservation of permanent teaching micro-capsule sample slice.
In permanent teaching with the preservation of micro-capsule sample slice, the entrance of air and glycerine are oozed out so that the easy quilt of slide
Microorganism pollution in air and go mouldy, cause micro-capsule sample to preserve for a long time.And for permanent teaching with slide come
Say, can for a long time preserve and be only key, but each relevant environmental conditions and other factors are to Saving specimen during Saving specimen
Influence it is huge, first, environmental factor：Impurity, dust, acid, alkali, the content of salt material and temperature in such as air, humidity,
The change of the factors such as illumination may all make sample be damaged；Second, biological factor：The microorganisms such as moth, bacterium, mould spread out
Damage caused by raw etc.；3rd, human factor：In use due to keeping, carry when caused by damage, or preservation and
Damage caused by servicing operations are lack of standardization, also has some incorrect methods when school microscope is observed to cause slide sample
Damage.Especially teaching slide, because frequency of use is high, students' experiences are shallow, are easily stained with trying on slide in use
Agent, water, dust etc., service life is short；And operated by student, easily the slide box for placing slide is integrally polluted, loss is tight
Weight.
The problem of for the above, develop a kind of permanent teaching micro-capsule sample slice and preparation method thereof, not only increase religion
Intuitive, and can preserve for a long time, it is very important.
The content of the invention
The first object of the present invention is that providing a kind of permanently teaching micro-capsule sample slice, the first object of the present invention exists
In a kind of preparation method of permanent teaching micro-capsule sample slice of offer.
The first object of the present invention is achieved in that described permanent teaching micro-capsule sample slice includes micro-capsule sample
Piece body and transparent vacuum container, described micro-capsule sample slice body include slide, the first cover glass, the second cover glass, micro-
Cystoblast, band, described microcapsule layer are arranged on slide, and the top of microcapsule layer covers the first cover glass, and described the
Being bonded between one cover glass and the second cover glass by neutral gum, the second described cover glass is more than the first cover glass,
Described slide is more than the second cover glass, and described band is used for the second cover glass including being arranged on the second cover glass surrounding
The band sealed between slide, described micro-capsule sample slice body are placed in transparent vacuum container.
The second object of the present invention, which is achieved in that, to be comprised the following steps：
1）The preparation of micro-capsule：Prepare the micro-capsule of micro-capsule sample slice to be produced；
2）Smear：Take 0.1~0.2g micro-capsules to be positioned among slide, 2~3 35% glycerine of drop are added dropwise, are sufficiently mixed uniformly；
3）First time mounting：Gently put down, avoid the occurrence of after first cover glass is contacted with 30 ~ 45° angle with liquid paraffin micro-capsule
Bubble, 35% unnecessary glycerine is sucked with blotting paper, and clean residual stain；
4）Second of mounting：3~5 drop neutral gum solution are added dropwise in the first cover glass center, then the second lid glass of horizontal covering
Piece, it is allowed to naturally sink with the extension of natural gum, as found there is bubble in mounting, avoids extruding, allow its to escape or use needle point naturally
Scalded after heating, escape bubble, make to remain without air between two layers of cover glass, sealing is complete；If surrounding has the tree oozed out
Glue, dip xylene solution using absorbent cotton and cleaned；
5）Dry and seal：The sample slice made is kept flat and spontaneously dried 5 ~ 10 days at room temperature, then using transparent neutral
Glass cement is uniformly applied to the second cover glass surrounding, makes it complete with slide sealing, cleans surface, labelled, is placed on
In transparent vacuum container, vacuumize, preserve, you can.
Compared with prior art, the present invention has following technique effect：
1st, the simple science of preparation method of the invention, the permanent teaching micro-capsule sample slice of preparation, observes shape under the microscope
Shape is completely clear, holding time length, as the religion auxiliary material of medical college pharmacy course, effectively increases the directly perceived of teaching
Property, improve the quality of teaching, while the sample slice of the present invention is simple in construction, can for a long time preserve, efficiently solves existing slide
The problem of easily going mouldy, being easily lost in teaching process.
2nd, sample slice of the invention does not interfere with microscopical observation not only, while can prevent entrance and the glycerine of air
Ooze out, ensure micro-capsule sample can preserve for a long time, do not gone mouldy by the microorganism pollution in air.
3rd, the setting of transparent vacuum container of the present invention has been inherently eliminated pollutant, mould and Air Temperature in air
Humidity etc. influences to caused by sample slice, and can be used as protective layer, avoids taking, carries the damage to caused by slide, most
Importantly, from need not taking out from vacuum environment and just can directly carry out, easy to use, it is lasting to preserve.
4th, the present invention can observe the vacuum in transparent vacuum container at any time by the vacuum meter of setting, be easy to slide
Management, can also observe sealing at any time, when there is the situation of sealing difference, be easy to find and handle in time.
5th, liquid paraffin oil is dyed red by the present invention before liquid paraffin micro-capsule is prepared with oil-soluble dyes tonyred,
Be advantageous to the observation under microscope after micro-capsule is formed, avoid being not easy to see because liquid paraffin oil and cyst membrane are all water white transparencies
Observe whether liquid paraffin oil is wrapped up by cyst membrane.
6th, mixing speed of the invention is 25~30 turns per minute, too low to be easily adhered the micro-capsule to be formed, too high easy
It is destroyed the micro-capsule to be formed；The addition of liquefied phenol of the present invention can also prevent going mouldy for sample.
Brief description of the drawings
Fig. 1 is the Making programme schematic diagram of the present invention；
Fig. 2 is the structural representation of the present invention；
In figure：1- slides, the cover glasses of 2- first, the cover glasses of 3- second, 4- microcapsule layers, 5- bands.
Embodiment
With reference to embodiment and accompanying drawing, the present invention is further illustrated, but the present invention is not subject in any way
Limitation, any conversion or replacement made based on the present invention, belongs to protection scope of the present invention.
Permanent teaching micro-capsule sample slice as shown in 1 ~ Fig. 2 of accompanying drawing, including micro-capsule sample slice body and transparent vacuum
Container, described micro-capsule sample slice body include slide 1, the first cover glass 2, the second cover glass 3, microcapsule layer 4, band 5,
Described microcapsule layer 4 is arranged on slide 1, and the top of microcapsule layer 4 covers the first cover glass 2, the first described cover glass 2
And second bonded by neutral gum between cover glass 3, the second described cover glass 3 is more than the first cover glass 2, described
Slide 1 is more than the second cover glass 3, and described band 5 is used for the second cover glass 3 including being arranged on the surrounding of the second cover glass 3
The band sealed between slide 1, described micro-capsule sample slice body are placed in transparent vacuum container.
The specification of the first described cover glass 2 is 18mm × 18mm, and the specification of the second cover glass 3 is 20mm × 20mm；Institute
The specification for the first cover glass 2 stated is 20mm × 20mm, and the specification of the second cover glass 3 is 22mm × 22mm.
Described band 5 also includes being arranged on the surrounding of the first cover glass 2 between the first cover glass 2 and slide 1
The band of sealing, described band 5 are transparent neutral glass cement band.
Described transparent vacuum container is clear glass vacuum box, described clear glass vacuum box include glass cartridge and
Glass top cover, the side of described glass cartridge are provided with vacuum pumping hole, on the inside of described glass top cover surrounding be provided with
The sealing joint strip of glass cartridge sealing.
The side of described glass cartridge is additionally provided with vacuum meter.
By neutral gum adhesion between the bottom surface of described glass cartridge and slide 1, described glass top cover it is interior
Side and the distance of the second cover glass 3 are 0 ~ 5mm.
The permanent teaching preparation method of micro-capsule sample slice of the present invention, comprises the following steps：
1）The preparation of micro-capsule：Prepare the micro-capsule of micro-capsule sample slice to be produced；
2）Smear：Take 0.1~0.2g micro-capsules to be positioned among slide, 2~3 35% glycerine of drop are added dropwise, are sufficiently mixed uniformly；
3）First time mounting：Gently put down, avoid the occurrence of after first cover glass is contacted with 30 ~ 45° angle with liquid paraffin micro-capsule
Bubble, 35% unnecessary glycerine is sucked with blotting paper, and clean residual stain；
4）Second of mounting：3~5 drop neutral gum solution are added dropwise in the first cover glass center, then the second lid glass of horizontal covering
Piece, it is allowed to naturally sink with the extension of natural gum, as found there is bubble in mounting, avoids extruding, allow its to escape or use needle point naturally
Scalded after heating, escape bubble, make to remain without air between two layers of cover glass, sealing is complete；If surrounding has the tree oozed out
Glue, dip xylene solution using absorbent cotton and cleaned；
5）Dry and seal：The sample slice made is kept flat and spontaneously dried 5 ~ 10 days at room temperature, then using transparent neutral
Glass cement is uniformly applied to the second cover glass surrounding, makes it complete with slide sealing, cleans surface, labelled, is placed on
In transparent vacuum container, vacuumize, preserve, you can.
The compound method of 35% described glycerine is to measure glycerine 35ml to add purified water that 2 drop liquefied phenols are added dropwise to 100ml
It is well mixed, you can.
The compound method of described neutral gum solution is to weigh neutral gum 10g, adds the stirring of 2~2.5ml dimethylbenzene
Dissolving is complete.
Step（1）Described in micro-capsule be liquid paraffin micro-capsule, the preparation method of described liquid paraffin micro-capsule include with
Lower step：
A, 3ml liquid paraffins oil is measured, is dyed with Sudan red dyes red stand-by；3g Arabic gums are weighed, are dissolved in 60ml purifying
In water, the liquid paraffin oil 3ml for adding red is placed in dispersing emulsification machine, is emulsified and is formed within 2~3 minutes liquid paraffin breast；
B, 3g gelatin is weighed, is added in 60ml purified waters after soaking swelling completely, is dissolved by heating in 60 DEG C of water-baths, gelatin is made
Solution；
C, above-mentioned liquid paraffin breast and gelatin solution are uniformly mixed, are placed in 45~50 DEG C of water-bath and are incubated, and always
It is stirred, it is 25~30 turns per minute to control mixing speed；
D, in whipping process, 10% acetum is added dropwise in the mixed liquor of liquid paraffin breast and gelatin solution, makes mixed liquor
PH forms micro-capsule when being 3.8~4.1；
E, the micro-capsule of formation is taken out from water-bath, adds 240ml purified waters to dilute, continue to stir, treat that temperature is down to 30~35 DEG C
When, move in ice bath, be stirred continuously the formalin 4ml of lower addition 37%, and 10% sodium hydroxide solution is added dropwise to solution
PH is 8~9, hardens the cyst membrane of micro-capsule, is finally filtered to remove liquid, obtains liquid paraffin micro-capsule.
The operation principle and the course of work of the present invention：The micro-capsule sample slice body of the present invention, is followed successively by load glass from bottom to top
Piece 1, microcapsule layer 4, the first cover glass 2, the second cover glass 3, pass through neutral gum between the first cover glass 2 and the second cover glass 3
Adhesion is carried out, the surrounding of the second cover glass 3 is provided with band 5 so that microcapsule layer 4 seals relative to the external world；
Further, band 5 can be set in the surrounding of the first cover glass 2, further improves sealing effectiveness, band 5 is adopted
With transparent neutral glass cement band, micro- sem observation is not influenceed, while can prevent the entrance of air and oozing out for glycerine, is protected
Card micro-capsule sample can preserve for a long time, not gone mouldy by the microorganism pollution in air；
Then micro-capsule sample slice body is placed in transparent vacuum container, keeps certain vacuum, fundamentally solved existing
The problems such as some slides easily go mouldy, when in use, directly permanent teaching is put with micro-capsule sample slice and seen under the microscope
Examine（Micro-capsule sample slice body is placed in transparent vacuum container）, it is easy to efficiently solve existing permanent teaching slide
Go mouldy, the problem of big is lost；And the transparent vacuum container of the present invention need not be picked and placeed and can directly observed, and efficiently solved and put
Put and the problem of easily causing whole box slide to be difficult to persist repeatedly is picked and placeed in kit；
Further, the vacuum in transparent vacuum container can be at any time observed by the vacuum meter of setting, is easy to slide
Management, when vacuum is less than certain numerical value, can be vacuumized, while when vacuum is 0 or nothing after vacuumize
When method keeps the vacuum of long period, illustrate that sealing is problematic, such as sealing joint strip defective tightness, be easy to be located in time
Reason.The transparent vacuum container of the present invention is also used as a protective case, further avoids polluting, extends the holding time of slide
And service life.
Embodiment 1
A kind of permanent teaching preparation method of micro-capsule sample slice, comprises the following steps：
1）The preparation of micro-capsule：Prepare the micro-capsule of micro-capsule sample slice to be produced；
2）Smear：Take 0.1g micro-capsules to be positioned among slide, 2 35% glycerine of drop are added dropwise, are sufficiently mixed uniformly；
3）First time mounting：Gently put down after first cover glass is contacted with 30 ° of angles with liquid paraffin micro-capsule, avoid the occurrence of gas
Bubble, 35% unnecessary glycerine is sucked with blotting paper, and clean residual stain；
4）Second of mounting：3 drop neutral gum solution are added dropwise in the first cover glass center, then the second cover glass of horizontal covering,
It is allowed to naturally sink with the extension of natural gum, as found there is bubble in mounting, avoids extruding, allow it to escape or be heated with needle point naturally
Scald afterwards, escape bubble, make to remain without air between two layers of cover glass, sealing is complete；If surrounding has the natural gum oozed out, make
Xylene solution is dipped with absorbent cotton to be cleaned；
5）Dry and seal：The sample slice made is kept flat and spontaneously dried 5 days at room temperature, then using transparent neutral glass
Glue is uniformly applied to the second cover glass surrounding, makes it complete with slide sealing, cleans surface, labelled, is placed on transparent
In vacuum tank, vacuumize, preserve, you can.
The compound method of 35% described glycerine is to measure glycerine 35ml to add purified water that 2 drop liquefied phenols are added dropwise to 100ml
It is well mixed, you can.
The compound method of described neutral gum solution is to weigh neutral gum 10g, and it is complete to add 2ml dimethylbenzene stirring and dissolvings
Entirely.
Described micro-capsule is liquid paraffin micro-capsule, and its preparation method comprises the following steps：
A, 3ml liquid paraffins oil is measured, is dyed with Sudan red dyes red stand-by；3g Arabic gums are weighed, are dissolved in 60ml purifying
In water, the liquid paraffin oil 3ml for adding red is placed in dispersing emulsification machine, is emulsified and is formed within 2 minutes liquid paraffin breast；
B, 3g gelatin is weighed, is added in 60ml purified waters after soaking swelling completely, is dissolved by heating in 60 DEG C of water-baths, gelatin is made
Solution；
C, above-mentioned liquid paraffin breast and gelatin solution are uniformly mixed, are placed in 45 DEG C of water-bath and are incubated, and carry out always
Stirring, it is 25 turns per minute to control mixing speed；
D, in whipping process, 10% acetum is added dropwise in the mixed liquor of liquid paraffin breast and gelatin solution, makes mixed liquor
PH forms micro-capsule when being 3.8；
E, the micro-capsule of formation is taken out from water-bath, adds 240ml purified waters to dilute, continue to stir, when temperature is down to 30 DEG C, moved
Into ice bath, the formalin 4ml of lower addition 37% is being stirred continuously, and the pH that 10% sodium hydroxide solution to solution is added dropwise is 8,
The cyst membrane of micro-capsule is hardened, is finally filtered to remove liquid, obtains liquid paraffin micro-capsule.
Embodiment 2
A kind of permanent teaching preparation method of micro-capsule sample slice, comprises the following steps：
1）The preparation of micro-capsule：Prepare the micro-capsule of micro-capsule sample slice to be produced；
2）Smear：Take 0.2g micro-capsules to be positioned among slide, 3 35% glycerine of drop are added dropwise, are sufficiently mixed uniformly；
3）First time mounting：Gently put down after first cover glass is contacted with 45° angle with liquid paraffin micro-capsule, avoid the occurrence of gas
Bubble, 35% unnecessary glycerine is sucked with blotting paper, and clean residual stain；
4）Second of mounting：5 drop neutral gum solution are added dropwise in the first cover glass center, then the second cover glass of horizontal covering,
It is allowed to naturally sink with the extension of natural gum, as found there is bubble in mounting, avoids extruding, allow it to escape or be heated with needle point naturally
Scald afterwards, escape bubble, make to remain without air between two layers of cover glass, sealing is complete；If surrounding has the natural gum oozed out, make
Xylene solution is dipped with absorbent cotton to be cleaned；
5）Dry and seal：The sample slice made is kept flat and spontaneously dried 10 days at room temperature, then using transparent neutral glass
Glass glue is uniformly applied to the second cover glass surrounding, makes it complete with slide sealing, cleans surface, labelled, is placed on
In bright vacuum tank, vacuumize, preserve, you can.
The compound method of 35% described glycerine is to measure glycerine 35ml to add purified water that 2 drop liquefied phenols are added dropwise to 100ml
It is well mixed, you can.
The compound method of described neutral gum solution is to weigh neutral gum 10g, adds 2.5ml dimethylbenzene stirring and dissolvings
Completely.
Described micro-capsule is liquid paraffin micro-capsule, and its preparation method comprises the following steps：
A, 3ml liquid paraffins oil is measured, is dyed with Sudan red dyes red stand-by；3g Arabic gums are weighed, are dissolved in 60ml purifying
In water, the liquid paraffin oil 3ml for adding red is placed in dispersing emulsification machine, is emulsified and is formed within 3 minutes liquid paraffin breast；
B, 3g gelatin is weighed, is added in 60ml purified waters after soaking swelling completely, is dissolved by heating in 60 DEG C of water-baths, gelatin is made
Solution；
C, above-mentioned liquid paraffin breast and gelatin solution are uniformly mixed, are placed in 50 DEG C of water-bath and are incubated, and carry out always
Stirring, it is 30 turns per minute to control mixing speed；
D, in whipping process, 10% acetum is added dropwise in the mixed liquor of liquid paraffin breast and gelatin solution, makes mixed liquor
PH forms micro-capsule when being 4.1；
E, the micro-capsule of formation is taken out from water-bath, adds 240ml purified waters to dilute, continue to stir, when temperature is down to 35 DEG C, moved
Into ice bath, the formalin 4ml of lower addition 37% is being stirred continuously, and the pH that 10% sodium hydroxide solution to solution is added dropwise is 9,
The cyst membrane of micro-capsule is hardened, is finally filtered to remove liquid, obtains liquid paraffin micro-capsule.
Embodiment 3
A kind of permanent teaching preparation method of micro-capsule sample slice, comprises the following steps：
1）The preparation of micro-capsule：Prepare the micro-capsule of micro-capsule sample slice to be produced；
2）Smear：Take 0.15g micro-capsules to be positioned among slide, 2 35% glycerine of drop are added dropwise, are sufficiently mixed uniformly；
3）First time mounting：Gently put down after first cover glass is contacted with 40 ° of angles with liquid paraffin micro-capsule, avoid the occurrence of gas
Bubble, 35% unnecessary glycerine is sucked with blotting paper, and clean residual stain；
4）Second of mounting：4 drop neutral gum solution are added dropwise in the first cover glass center, then the second cover glass of horizontal covering,
It is allowed to naturally sink with the extension of natural gum, as found there is bubble in mounting, avoids extruding, allow it to escape or be heated with needle point naturally
Scald afterwards, escape bubble, make to remain without air between two layers of cover glass, sealing is complete；If surrounding has the natural gum oozed out, make
Xylene solution is dipped with absorbent cotton to be cleaned；
5）Dry and seal：The sample slice made is kept flat and spontaneously dried 7 days at room temperature, then using transparent neutral glass
Glue is uniformly applied to the second cover glass surrounding, makes it complete with slide sealing, cleans surface, labelled, is placed on transparent
In vacuum tank, vacuumize, preserve, you can.
The compound method of 35% described glycerine is to measure glycerine 35ml to add purified water that 2 drop liquefied phenols are added dropwise to 100ml
It is well mixed, you can.
The compound method of described neutral gum solution is to weigh neutral gum 10g, adds 2.2ml dimethylbenzene stirring and dissolvings
Completely.
Described micro-capsule is liquid paraffin micro-capsule, and its preparation method comprises the following steps：
A, 3ml liquid paraffins oil is measured, is dyed with Sudan red dyes red stand-by；3g Arabic gums are weighed, are dissolved in 60ml purifying
In water, the liquid paraffin oil 3ml for adding red is placed in dispersing emulsification machine, is emulsified and is formed within 2 minutes liquid paraffin breast；
B, 3g gelatin is weighed, is added in 60ml purified waters after soaking swelling completely, is dissolved by heating in 60 DEG C of water-baths, gelatin is made
Solution；
C, above-mentioned liquid paraffin breast and gelatin solution are uniformly mixed, are placed in 48 DEG C of water-bath and are incubated, and carry out always
Stirring, it is 28 turns per minute to control mixing speed；
D, in whipping process, 10% acetum is added dropwise in the mixed liquor of liquid paraffin breast and gelatin solution, makes mixed liquor
PH forms micro-capsule when being 4.0；
E, the micro-capsule of formation is taken out from water-bath, adds 240ml purified waters to dilute, continue to stir, treat that temperature is down to 30~35 DEG C
When, move in ice bath, be stirred continuously the formalin 4ml of lower addition 37%, and 10% sodium hydroxide solution is added dropwise to solution
PH is 8, hardens the cyst membrane of micro-capsule, is finally filtered to remove liquid, obtains liquid paraffin micro-capsule.
Claims (10)
1. micro-capsule sample slice is used in a kind of permanent teaching, it is characterised in that including micro-capsule sample slice body and transparent vacuum container,
Described micro-capsule sample slice body includes slide（1）, the first cover glass（2）, the second cover glass（3）, microcapsule layer（4）, sealing
Band（5）, described microcapsule layer（4）It is arranged on slide（1）On, and microcapsule layer（4）Top cover the first cover glass（2）, institute
The first cover glass stated（2）With the second cover glass（3）Between bonded by neutral gum, the second described cover glass（3）
More than the first cover glass（2）, described slide（1）More than the second cover glass（3）, described band（5）Including being arranged on
Second cover glass（3）Surrounding is used for the second cover glass（3）With slide（1）Between the band that seals, described micro-capsule sample
Piece body is placed in transparent vacuum container.
2. micro-capsule sample slice is used in permanent teaching according to claim 1, it is characterised in that the first described cover glass（2）
Specification be 18mm × 18mm, the second cover glass（3）Specification be 20mm × 20mm；The first described cover glass（2）Specification
For 20mm × 20mm, the second cover glass（3）Specification be 22mm × 22mm.
3. micro-capsule sample slice is used in permanent teaching according to claim 1, it is characterised in that described band（5）Also wrap
Include and be arranged on the first cover glass（2）Surrounding is used for the first cover glass（2）With slide（1）Between the band that seals, it is described
Band（5）For transparent neutral glass cement band.
4. micro-capsule sample slice is used in permanent teaching according to claim 1, it is characterised in that described transparent vacuum container
For clear glass vacuum box, described clear glass vacuum box includes glass cartridge and glass top cover, described glass cartridge
Side is provided with vacuum pumping hole, and surrounding is provided with the sealing joint strip sealed with glass cartridge on the inside of described glass top cover.
5. micro-capsule sample slice is used in permanent teaching according to claim 4, it is characterised in that the side of described glass cartridge
Face is additionally provided with vacuum meter.
6. micro-capsule sample slice is used in permanent teaching according to claim 4, it is characterised in that the bottom of described glass cartridge
Face and slide（1）Between pass through neutral gum adhesion, the inner side of described glass top cover and the second cover glass（3）Distance be
0~5mm。
7. a kind of preparation method according to any described permanent teaching micro-capsule sample slice of claim 1 ~ 6, its feature exists
In comprising the following steps：
1）The preparation of micro-capsule：Prepare the micro-capsule of micro-capsule sample slice to be produced；
2）Smear：Take 0.1~0.2g micro-capsules to be positioned among slide, 2~3 35% glycerine of drop are added dropwise, are sufficiently mixed uniformly；
3）First time mounting：Gently put down, avoid the occurrence of after first cover glass is contacted with 30 ~ 45° angle with liquid paraffin micro-capsule
Bubble, 35% unnecessary glycerine is sucked with blotting paper, and clean residual stain；
4）Second of mounting：3~5 drop neutral gum solution are added dropwise in the first cover glass center, then the second lid glass of horizontal covering
Piece, it is allowed to naturally sink with the extension of natural gum, as found there is bubble in mounting, avoids extruding, allow its to escape or use needle point naturally
Scalded after heating, escape bubble, make to remain without air between two layers of cover glass, sealing is complete；If surrounding has the tree oozed out
Glue, dip xylene solution using absorbent cotton and cleaned；
5）Dry and seal：The sample slice made is kept flat and spontaneously dried 5 ~ 10 days at room temperature, then using transparent neutral
Glass cement is uniformly applied to the second cover glass surrounding, makes it complete with slide sealing, cleans surface, labelled, is placed on
In transparent vacuum container, vacuumize, preserve, you can.
8. the preparation method of permanent teaching micro-capsule sample slice according to claim 7, it is characterised in that described 35%
The compound method of glycerine is to measure glycerine 35ml to add purified water that 2 drop liquefied phenols are added dropwise and are well mixed to 100ml, you can.
9. the preparation method of permanent teaching micro-capsule sample slice according to claim 7, it is characterised in that in described
Property gum-solution compound method be to weigh neutral gum 10g, add 2~2.5ml dimethylbenzene stirring and dissolvings it is complete.
10. the preparation method of permanent teaching micro-capsule sample slice according to claim 7, it is characterised in that step（1）
Described in micro-capsule be liquid paraffin micro-capsule, the preparation method of described liquid paraffin micro-capsule comprises the following steps：
A, 3ml liquid paraffins oil is measured, is dyed with Sudan red dyes red stand-by；3g Arabic gums are weighed, are dissolved in 60ml purifying
In water, the liquid paraffin oil 3ml for adding red is placed in dispersing emulsification machine, is emulsified and is formed within 2~3 minutes liquid paraffin breast；
B, 3g gelatin is weighed, is added in 60ml purified waters after soaking swelling completely, is dissolved by heating in 60 DEG C of water-baths, gelatin is made
Solution；
C, above-mentioned liquid paraffin breast and gelatin solution are uniformly mixed, are placed in 45~50 DEG C of water-bath and are incubated, and always
It is stirred, it is 25~30 turns per minute to control mixing speed；
D, in whipping process, 10% acetum is added dropwise in the mixed liquor of liquid paraffin breast and gelatin solution, makes mixed liquor
PH forms micro-capsule when being 3.8~4.1；
E, the micro-capsule of formation is taken out from water-bath, adds 240ml purified waters to dilute, continue to stir, treat that temperature is down to 30~35 DEG C
When, move in ice bath, be stirred continuously the formalin 4ml of lower addition 37%, and 10% sodium hydroxide solution is added dropwise to solution
PH is 8~9, hardens the cyst membrane of micro-capsule, is finally filtered to remove liquid, obtains liquid paraffin micro-capsule.
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