CN201785336U - In-situ-hybridization one-step mounting tape - Google Patents
In-situ-hybridization one-step mounting tape Download PDFInfo
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- CN201785336U CN201785336U CN2009200830632U CN200920083063U CN201785336U CN 201785336 U CN201785336 U CN 201785336U CN 2009200830632 U CN2009200830632 U CN 2009200830632U CN 200920083063 U CN200920083063 U CN 200920083063U CN 201785336 U CN201785336 U CN 201785336U
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- mounting
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Abstract
The utility model belongs to the technical field of molecular cytobiology, and relates to a one-step mounting tape which is used for an in-situ-hybridization experiment, which aims at overcoming the weaknesses of the present mounting technology and providing a mounting method which is convenient to use and has high safety and good hybridization effect. The tape consists of PVC (poly vinyl chloride) film (1), polymeric adhesives (3), clean circular microscopic glass with diameter of 1.5 cm (thickness of 0.017 cm) (4) and waxy cover paper (5). The in-situ-hybridization one-step mounting tap has the following advantages that: 1. no toxicity and harm exist; 2. no influence exists on the hybridization solution; 3. the operation is simple and convenient, so time can be saved; 4. mounting position and mounting effect can be better controlled; 5. the hybridization specimen is free from being flaked because of the pulling and movement of the microscopic glass; 6. the standardization degree is high, so the mounting tape is suitable for the mass experiment operation.
Description
Technical field
The utility model belongs to the molecular cytobiology technical field, is specifically related to the crossing phase of hybrid experiment in position, replaces traditional method, can will add cover glass and mounting step simultaneously quick and go on foot the adhesive tape of finishing.
Background technology
In situ hybridization is an experimental technique that application is extremely wide, have very high susceptibility and specificity, be not only cytobiology, molecular biological important research means, and entered the clinical experiment diagnostic field, be applied to fine hair and amniotic fluid interval cell carries out antenatal diagnosis as fluorescence in situ hybridization (FISH) technology.Its ultimate principle is under suitable condition, utilize base complementrity paired rule is arranged between the single stranded nucleic acid molecule, single-chain nucleic acid (DNA or RNA) with distinguished sequence is that probe combines with the interior nucleic acid renaturation to be measured of cell, then the technology that determined nucleic acid is shown on original position in cell by the marker on the probe.The probe mark thing can be divided into isotropic substance and heterotope two classes, because the isotopic labeling probe has radioactivity, existence is to the harm of environment and human body and be subjected to shortcomings such as transformation period restriction, now seldom use, but not the isotopic labeling probe developed rapidly in recent years, and its marker mainly contains fluorescence, vitamin H and digoxin etc.What the utility model mainly was suitable for is non-isotopic labeling probe.
In situ hybridization is different with marker according to the kind of nucleic acid probe, concrete experimental technique also has certain difference, but its basic skills and principle are roughly the same, mainly comprise: 1. prepare before the hybridization, comprise draw materials, slide and histiocytic processing, fixing, by operations such as enzymic digestion strengthen the subsequent experimental middle probe penetrance, lower background etc.; 2. hybridization; 3. hybridize aftertreatment; 4. show.Hybridization is its core procedure, comprises hybridization solution (by probe, hybridization buffer and H
2O forms) drip hybridization region on slide glass sample surfaces, add cover glass, mounting, hatch this several steps.The purpose that adds cover glass, the one, cover glass can make limited hybridization solution contact with big as far as possible hybridization is regional more fully; The 2nd, prevent the evaporation that temperature in the process causes hybridization solution of hatching of long period, guarantee the moist environment that hybridization is required.Owing to there is slit around the cover glass with the air traffic, still can cause the hybridization solution evaporation in the process hatching for a long time, so routine also will be carried out mounting both at home and abroad, promptly the hybridization solution evaporation is thoroughly avoided in the slit between closing cap slide and slide glass.The most frequently used method is to coat mountant Rubber Cement around the cover glass, also has a few peoples to add material such as whiteruss around cover glass and carries out sealing.But Rubber Cement is a kind of tackiness agent of organic solvent dissolving untreated rubber, has certain toxicity, for a long time, contact in a large number harmful, and utilize Rubber Cement mounting, because its viscosity is big, causes and a cover glass will be sealed length consuming time, bad or careless slightly as technology, it is of low quality often to cause mounting; And utilizing the whiteruss mounting, under the high temperature when hybridization, whiteruss melts the pollution that very easily causes hybridization solution, influences crossbreeding effect.Also there are the Individual testwas personnel after adding cover glass, to seal film parcel and twine mounting, play good sealing effectiveness but this mounting method is more difficult by Parafilm.Above-mentioned the whole bag of tricks hybridization finishes and all needs to remove respectively carefully mountant/film and cover glass, and since have between cover glass and slide glass hybridization region that adsorptive power is careless slightly may be because of the dragging displacement and cause hybridization sample flake of cover glass, and red tape like this has also caused difficulty to the operation of batch samples.
Summary of the invention
The purpose of this utility model is the deficiency that overcomes existing mounting technology, for hybridization in situ experiment provides a kind of easy to use, safe, quick and good single step mounting adhesive tape of crossbreeding effect.
The technical solution adopted in the utility model is: a kind of single step mounting adhesive tape, be the 2.5cmX2.5cm square substantially, and thick 0.02cm-0.04cm cooperates macromolecule adhesive as mounting adhesive tape foundation structure with the PVC film.Adhesive tape is a toughness only.At the middle part of adhesive tape adhesive faces, the circular lid slide (thickness 0.017cm) of a diameter 1.5cm cleaning is arranged, cover glass and PVC film close are bonded into an one-piece construction.Whole adhesive tape adhesive faces is covered by thick 0.01cm wax covering paper, and the effect of this wax covering paper has two: one, prevent the mounting adhesive tape when storing and wrapping paper stick together mutually, the 2nd, can keep the cleaning of cover glass.Face with the preceding wax covering paper that tears off like a cork and can use adhesive tape; One side of adhesive tape is designed to lune adhesion without adhesiver position, and hand grips when being beneficial to operation.PVC tape is transparent, carries out mounting with the hybridization region on the corresponding slide glass in position, cover glass place during mounting, can accurately will add cover glass and mounting two steps unification; Because PVC tape and cover glass all are transparent, also can observe the mounting effect at any time in the mounting process, as have or not bubble generation etc.Hybridization finishes, the slide glass of can holding steadily on the other hand, two fingers of another hand pinch lune adhesion without adhesiver position and exert oneself gently, mounting adhesive tape whole removing can be removed, need not be again as traditional operation, lose time except that on the cover glass in removal mountant and folder, such operation can not produce any influence to hybridization sample on the slide glass.PVC tape viscosity is lasting, waterproof, chance water difficult drop-off, helps crossover process and finishes smoothly.
This mounting adhesive tape can every is an independent packaging, conveniently produces, transhipment, sells, stores and use.
Compared with prior art, the utlity model has following advantage: 1. nontoxic; 2. the hybridization solution chemical ingredients there is not influence; 3. adding one step of cover glass and mounting finishes, during removal also a step finish, every sheet operation only needs several seconds, and is time saving and energy saving; 4. cover glass and adhesive tape are one-piece constructions, are beneficial to and hold the mounting position better; 5. can not cause hybridization sample flake because of the displacement that drags of cover glass; 6. the standardization level height is fit to experimental implementation in enormous quantities (we use through hundreds of examples are actual, and effect is excellent); 7. as scale operation of mounting adhesive tape and use, its cost is significantly less than present traditional method.
Description of drawings
Fig. 1 is an adhesive faces structural representation of the present utility model
Fig. 2 is a side of the present utility model cross-sectional schematic
Fig. 3 is an application principle schematic top plan view of the present utility model
Fig. 4 is the utility model application principle side cross-sectional schematic
Wherein: the 1-PVC film; 2-does not have the viscose glue holding area; The 3-sticky region; The 4-cover glass; 5-wax covering paper; The 6-slide glass; 7-slide glass numerical markings district; 8-sample hybridization region
Embodiment
In conjunction with the accompanying drawings embodiment of the present utility model is described further: single step mounting adhesive tape roughly is square, wide 2.5cm.At its positive middle part, there is the circular lid slide 4 (thickness 0.17mm) of a diameter 1.5cm bonding in aggregates with the PVC film close, on the PVC film of cover glass peripheral region macromolecule adhesive 3 is arranged, covered by covering paper 5 before using, there is a lune adhesion without adhesiver holding area 2 on the right side; It is special that the single step mounting adhesive tape back side does not have, and promptly the PVC film 1.Finger pinches no viscose glue holding area 2 during mounting, tears covering paper 5 off, exposes the adhesive area 3 it under, with dripped on the cover glass 4 aligning slide glasss 6 probe sample hybridization region 8, gently pressure activated cohesive district 3 makes itself and the bonding mounting step of promptly having finished of slide glass; End is hatched in hybridization, hand slide glass 6 of holding steadily, and the another hand finger pinches adhesion without adhesiver holding area 2, firmly is about to mounting adhesive tape (comprising cover glass 4) whole removing gently and removes, and is very convenient.
Claims (5)
1. the mounting adhesive tape of a hybridization in situ experiment, it is characterized in that: cooperate macromolecule adhesive (3) as adhesive tape foundation structure with PVC film (1), adhesive tape is a toughness only, adhesive faces is covered by covering paper (5) fully before the adhesive faces middle part has a circular lid slide (4) and PVC film close to be bonded into an one-piece construction, uses.
2. mounting adhesive tape according to claim 1 is characterized in that: PVC film (1), macromolecule adhesive (3), cover glass (4) all are transparent materials.
3. mounting adhesive tape according to claim 1 is characterized in that: circular lid slide (4) cleaning, diameter 1.5cm, thick 0.017cm.
4. mounting adhesive tape according to claim 1 is characterized in that: a side of adhesive tape has one to be lune position (2), and the two sides is all inviscid.
5. mounting adhesive tape according to claim 1 is characterized in that: covering paper (5) is a wax paper, can easily remove from the adhesive tape adhesive faces.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200830632U CN201785336U (en) | 2009-08-03 | 2009-08-03 | In-situ-hybridization one-step mounting tape |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009200830632U CN201785336U (en) | 2009-08-03 | 2009-08-03 | In-situ-hybridization one-step mounting tape |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201785336U true CN201785336U (en) | 2011-04-06 |
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ID=43817311
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009200830632U Expired - Fee Related CN201785336U (en) | 2009-08-03 | 2009-08-03 | In-situ-hybridization one-step mounting tape |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201785336U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110553888A (en) * | 2019-09-16 | 2019-12-10 | 中国农业科学院特产研究所 | Method for rapid tabletting of oocyte after immunofluorescence staining |
-
2009
- 2009-08-03 CN CN2009200830632U patent/CN201785336U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110553888A (en) * | 2019-09-16 | 2019-12-10 | 中国农业科学院特产研究所 | Method for rapid tabletting of oocyte after immunofluorescence staining |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110406 Termination date: 20150803 |
|
| EXPY | Termination of patent right or utility model |