CN1371989A - High efficiency safety biological integral mounting method - Google Patents
High efficiency safety biological integral mounting method Download PDFInfo
- Publication number
- CN1371989A CN1371989A CN 01114602 CN01114602A CN1371989A CN 1371989 A CN1371989 A CN 1371989A CN 01114602 CN01114602 CN 01114602 CN 01114602 A CN01114602 A CN 01114602A CN 1371989 A CN1371989 A CN 1371989A
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- China
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- pad
- cover glass
- hole
- transparent
- mounting method
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Between glass slide and cover glass a layer of transparent gasket protection layer whose thickkess is slightly thicker than that of specimen and whose centre is holed in added, and its concrete method includes the following steps: placing a transparent gasket which is clean, identical to cover glass is size and is equipped with a hole in its centre on the glass slide, placing the mounting medium and specimen into the hole of the gasket, covering the gasket with cover plass and sealing so as to implement said invention.
Description
The present invention relates to biological integral mounting method, the microorganism belonging to genus field.
Existing integral mounting technology (Li Zhengli, 1987, " plant tabletting technology " (second edition) Science Press) be wanting the sample of sealing to be fixed on the slide glass with mounting medium, direct covered in the above then, unnecessary mounting medium is inhaled with thieving paper and is gone around the cover glass, and is coated with envelope with sealing agent.Therefore, sealing agent directly contacts with mounting medium, because both have certain phase solubility, is difficult for playing the ideal sealing function.In addition, cover glass directly is pressed on the sample of envelope Tibetan, soft some fragile envelopes are hidden sample, after the mounting, when the sealing agent drying shrinkage, with the downward tension and compression of cover glass, easily this class is sealed the sample compressive strain of Tibetan until breaking, simultaneously, the mounting medium that is extruded is easily spilt uneven local the squeezing of sealing agent, does not reach the ideal sealing effectiveness.
It is sealed easily to the purpose of this invention is to provide a kind of mounting medium, can safe storage to the sample hidden of seal, be difficult for producing extruding, distortion, the existing microorganism integral mounting method of disruptive.
Technical scheme of the present invention is to increase one deck to be slightly thicker than sample thickness, the transparent pad protective layer of middle perforate between slide glass and cover glass.Concrete method is: clean, middle perforate, the size transparent pad consistent with cover glass are placed on the slide glass, add that a little mounting medium is in the pad hole.The biological sample that will seal up for safekeeping is placed in the pad hole again, covers with cover glass, and cover glass is alignd with pad.Unnecessary mounting medium blots with thieving paper around the cover glass, and is coated with envelope with sealing agent.Transparent pad can be various transparent plastics, also can be glass, and preferred version is a polyethylene sheets.The shape of pad mesopore can freely be selected, and preferred version is garden shape or Sui Yuan shape.The diameter in hole or diagonal lines can 5～15mm, and preferred version is 10～12mm.Also can be earlier when concrete sealing operation half of cover glass be covered on transparent pad, after will treat that the sample of integral mounting is placed in the above-mentioned pad hole sealing liquid, the sample adjustment that envelope is hidden is slowly moved cover glass therebetween, and cover glass is alignd with pad.Unnecessary mounting medium blots with thieving paper around the cover glass, and is coated with envelope with sealing agent, as shown in the figure.
Sealing agent can be by the formulation of following routine:
Flake lac.。。。。。。。。。。。。。。。。。。。。。。230mg
Raw spirit.。。。。。。。。。。。。。。。。。。。。。287ml
Viscotrol C.。。。。。。。。。。。。。。。。。。。。。。2.13ml
Saturated frankincense gum spirituous solution.。。。。。。。。。。。。。。。。2.13ml
Mounting medium can be by the formulation of following routine:
Glycerine.。。。。。。。。。。。。。。。。。。。。。。。。10ml
Sterilized water.。。。。。。。。。。。。。。。。。。。。。。。10ml
70% alcohol.。。。。。。。。。。。。。。。。。。。。。。。。30ml
The present invention is limited in the hole of cover glass intermediate washer mounting medium, plays mounting medium and sealing agent are separated, and is not in contact with one another and influences.Like this, the sealing agent that is coated with envelope really plays the effect of sealing.In addition, because the thickness of pad is more bigger than the seal sample thickness of hiding, after the mounting; when the sealing agent drying shrinkage, when cover glass was strained downwards, this pad just played the seal safeguard protection effect of hiding sample; the compressing of having avoided cover glass that envelope is hidden sample causes distortion even breaks.Simultaneously, mounting medium can not be extruded yet and spill, and reaches the purpose of permanent seal.This is to the type specimen on some rare samples, particularly taxonomy, and is particularly important, for the descendant restudies, investigates these materials science card a tree name is provided.
The drawing explanation:
Accompanying drawing is biological integral mounting figure
Among the figure, 1---slide glass
2---transparent pad (down) and cover glass (last)
3---hole and mounting medium that transparent pad is opened
4---biological sample
5---sealing agent
Embodiment:
Clean, middle opening diameter 12mm, the size polyethylene transparent pad consistent with cover glass are placed on the slide glass.Add that a little mounting medium is in the pad hole.Half of cover glass covered on transparent pad, after will treat that the sample of integral mounting is placed in the above-mentioned pad hole sealing liquid, the sample adjustment that envelope is hidden is slowly moved cover glass therebetween, and cover glass is alignd with pad.Unnecessary mounting medium blots with thieving paper around the cover glass, and is coated with envelope with sealing agent, as shown in the figure.
Claims (8)
1. high efficiency safety biological integral mounting method, it is characterized in that increasing between slide glass and the cover glass the transparent pad protective layer that one deck is slightly thicker than sample thickness, middle perforate, concrete mounting process is: clean, middle perforate, the size transparent pad consistent with cover glass are placed on the slide glass, add that a little mounting medium is in the pad hole; The biological sample that will seal up for safekeeping is placed in the pad hole again, covers with cover glass, and cover glass is alignd with pad, or earlier half of cover glass is placed on pad above, after the biological sample that will seal up for safekeeping be placed in the pad hole, removable cover slide slowly makes cover glass align with pad then; Unnecessary mounting medium blots with thieving paper around the cover glass, and is coated with envelope with sealing agent.
2. according to the integral mounting method described in the claim 1, it is characterized in that transparent pad is selected from a kind of in various transparent plastic sheets, the sheet glass.
3. according to the integral mounting method described in the claim 2, it is characterized in that transparent pad preferred version is a polyethylene.
4. according to the integral mounting method described in the claim 1,2 or 3, it is characterized in that the shape in the middle hole of being opened of transparent pad is arbitrarily.
5. according to the integral mounting method described in the claim 4, it is characterized in that the preferred version of the shape in the middle hole of being opened of transparent pad is garden shape or Sui Yuan shape.
6. according to the integral mounting method described in the claim 1～5, it is characterized in that the diameter in the hole of being opened in the middle of the transparent pad or diagonal lines are between 5～15mm.
7. according to the integral mounting method described in the claim 6, it is characterized in that the diameter in the hole of being opened in the middle of the transparent pad or cornerwise preferred version are between 10～12.
8. according to the integral mounting method described in the claim 1, it is characterized in that clean, middle opening diameter 12mm, the size transparent polyethylene pad consistent with cover glass are placed on the slide glass; Add that a little mounting medium is in the pad hole; Half of cover glass covered on transparent pad, after will treat that the sample of integral mounting is placed in the above-mentioned pad hole sealing liquid, the sample adjustment that envelope is hidden is slowly moved cover glass therebetween, and cover glass is alignd with pad; Unnecessary mounting medium blots with thieving paper around the cover glass, and is coated with envelope with sealing agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011146028A CN1150312C (en) | 2001-04-05 | 2001-04-05 | High efficiency safety biological integral mounting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011146028A CN1150312C (en) | 2001-04-05 | 2001-04-05 | High efficiency safety biological integral mounting method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1371989A true CN1371989A (en) | 2002-10-02 |
| CN1150312C CN1150312C (en) | 2004-05-19 |
Family
ID=4661237
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011146028A Expired - Fee Related CN1150312C (en) | 2001-04-05 | 2001-04-05 | High efficiency safety biological integral mounting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1150312C (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445071A (en) * | 2015-12-23 | 2016-03-30 | 中国人民解放军第三军医大学第一附属医院 | Section sealing box |
| CN105784661A (en) * | 2016-04-06 | 2016-07-20 | 北京雷根生物技术有限公司 | Mounting medium capable of reducing fluorescence decay |
| CN106244513A (en) * | 2016-07-26 | 2016-12-21 | 北京林业大学 | A kind of vacuole that separates is distributed and the method for motion with albumen on observation tonoplast |
| CN106840830A (en) * | 2017-01-24 | 2017-06-13 | 宁夏大学 | The mounting method and mounting operation device of Permanent slide |
| CN107798981A (en) * | 2017-10-31 | 2018-03-13 | 楚雄医药高等专科学校 | A kind of permanent teaching micro-capsule sample slice and preparation method thereof |
| CN108359620A (en) * | 2018-02-26 | 2018-08-03 | 巢湖学院 | A kind of Gram's staining Quality Control piece and preparation method thereof and application |
-
2001
- 2001-04-05 CN CNB011146028A patent/CN1150312C/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105445071A (en) * | 2015-12-23 | 2016-03-30 | 中国人民解放军第三军医大学第一附属医院 | Section sealing box |
| CN105445071B (en) * | 2015-12-23 | 2018-09-07 | 中国人民解放军第三军医大学第一附属医院 | mounting box |
| CN105784661A (en) * | 2016-04-06 | 2016-07-20 | 北京雷根生物技术有限公司 | Mounting medium capable of reducing fluorescence decay |
| CN105784661B (en) * | 2016-04-06 | 2021-01-01 | 北京雷根生物技术有限公司 | Sealing piece agent for reducing fluorescence attenuation |
| CN106244513A (en) * | 2016-07-26 | 2016-12-21 | 北京林业大学 | A kind of vacuole that separates is distributed and the method for motion with albumen on observation tonoplast |
| CN106840830A (en) * | 2017-01-24 | 2017-06-13 | 宁夏大学 | The mounting method and mounting operation device of Permanent slide |
| CN107798981A (en) * | 2017-10-31 | 2018-03-13 | 楚雄医药高等专科学校 | A kind of permanent teaching micro-capsule sample slice and preparation method thereof |
| CN108359620A (en) * | 2018-02-26 | 2018-08-03 | 巢湖学院 | A kind of Gram's staining Quality Control piece and preparation method thereof and application |
| CN108359620B (en) * | 2018-02-26 | 2020-09-01 | 巢湖学院 | Gram chromatin control tablet and its making method and use |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1150312C (en) | 2004-05-19 |
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Granted publication date: 20040519 Termination date: 20120405 |