CN1645135A - Rapid inspection for nematode larva of dormant pine wood - Google Patents
Rapid inspection for nematode larva of dormant pine wood Download PDFInfo
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- CN1645135A CN1645135A CN 200510018185 CN200510018185A CN1645135A CN 1645135 A CN1645135 A CN 1645135A CN 200510018185 CN200510018185 CN 200510018185 CN 200510018185 A CN200510018185 A CN 200510018185A CN 1645135 A CN1645135 A CN 1645135A
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Abstract
A quick quaratine method of dormant nematoda larva in pine wood includes cutting wood into pieces and packing them by etamine to put it in container with culture solution, placing the container at 20-41 deg.c for separation culture, filtering the solution with 100 M and 500M screen mesh and collecting nematode from the solution by 500m screen mesh for being detected with microscope. The culture solution is prepared as using hydrogen peroxide as base solution then adding one or multiple of nutrition solution, gibberellin, potassium nitrate and clenbuterol hydrochloride for combination.
Description
Technical field
The present invention relates to a kind of rapid inspection for nematode larva of dormant pine wood.
Background technology
Pine nematode (You claiming pine tree wilt disease) is a kind of destructive disease on the pine tree. Wound when this nematode Zhu will supplement the nutrients by Monochamus alternatus enters xylem, colonize in resin canal Zhong, move when Zai is prolific, and spread all over complete stool, and causing resin canal parenchyma cell and epithelial destruction and death, Zao becomes Zhi Zhu dehydration, Zheng rises Zuo Yong and reduces, resin secretion sharply reduces and stops, and pine needle becomes yellowish-brown or bronzing from celadon, Zui after Zheng Zhu withered. Host plant infects pine wood nematode and got final product dead in 40 days after being ill, from the time that Zhi Zhu falls ill and destroys Zhi need 3-5 to Zheng sheet pine forest, You Yu pine wood nematode pathogenicity is strong, propagate fast, the dead speed of host is fast, and usually Cu is not as good as anti-, in case occur, except the sick tree of felling, there is no at present the way of radical cure, become huge loss not only for national economy Zao, also destroyed the right view of Zi and ecological environment, the more important thing is the serious threat of formation to Zhu name scenic spot, world's natural character legacy and Chong point ecotope such as Yue 8.5 hundred million mu of China (5,710 ten thousand hectares) Zhen leaf tree resources and Mount Huang, Zhang Jiajie, Mount Lushan.
At present, the countries and regions You generations such as pine nematode Zhi Zai Japan, the U.S., Canada, Mexico, Korea S, Korea, Portugal, Hong Kong, Taiwan. China has diffused to some areas that economize in Shandong, Jiangsu, Zhe river, Anhui, Guangdong etc. at present since Zai Nanjing Zhong lofty mountains in 1882 are found pine nematode first, Zhi meets economic loss 2,500,000,000 Yuan, indirect loss 250,000,000,000 Yuan.
After the China joined WTO, international trade comes more frequent and more frequent, and the wood package materials of passing in and out with goods comes more and more, and Zhe provides chance for pine wood nematode discrepancy China. Spread out of the border in order to prevent that pine wood nematode from importing into Wooden package, since 1999, each entry and exit inspection and quarantine bureau of China has been strengthened the quarantine to the Wooden package of passing in and out, each forest plants quarantine station of the whole nation has also strengthened the inspection and quarantine work to epidemic-stricken area allocation and transportation goods Wooden package, but Zai quarantine Zhong finds, existing quarantine method You Yu takes length, 4-5 days at least (materials that nematode levels is many), at most (material that nematode levels is few) more than 10 days, can satisfy far from the needs of port and domestic forest plants quick test quarantine, its Yuan is because being: 1, from wood package materials Zhi connect separate mostly be greatly the decentralized third-instar larvae, and the morphological feature of Main Basis pine wood nematode adult is identified at present, decentralized third-instar larvae or persistent form four-age larva to be cultivated as adult, be needed the regular hour; 2, the difference of the situations such as the resting period of inward wood package materials, gradient of infection, water content, quarantine processing, Zai Import quarantine process Zhong, the pine wood nematode situation seldom that separation often occurs, several of the Zhi You of You, the You worm Zhuan of resting state that will Zhe is few in number becomes for the adult of identifying, according to existing method Yu longer time (more than 10 days) of Technology Need, bring many troubles for harbour, harbour, inspection post, the owner of cargo, testing agency, as to by the time quarantine the result out after the Zai clearance of being open to the custom, Ze Zao becomes long-pending ship to overstock a harbour, and Zhi connects impact externally and domestic trade; As not waiting quarantine result out take to be open to the custom first mode of rear quarantine, Ze You possibility Zao becomes undetected or the epidemic situation diffusion; As Zai quarantine Zhong with suspicious nematode not Zuo further deeply the check, it is biological just to be judged to general You evil, and Ze You possibility Zao becomes the invasion of pine wood nematode, as suspect object is judged to pine wood nematode, Ze may causes trade dispute, affects state-to-state relations and internal and external trade. Yu is the fast detecting problem of present port and forest plants quarantine mechanism pine wood nematode in the urgent need to address.
According to the history of life of pine wood nematode, pine wood nematode can be divided into 1 instar larvae, 2 instar larvaes, 3 instar larvaes, 4 instar larvaes, adult; Or 1 instar larvae, 2 instar larvaes, decentralized 3 instar larvaes, persistent form 4 instar larvaes, adult. Can be without the 4 instar larvae stages of persistent form under the certain condition of decentralized 3 instar larvae Zai and Zhi switch through and be reproduction type 4 instar larvaes, Zai Zhuan becomes adult. Under the environmental condition of pine wood nematode Zai optimum, a breeding generation only needs 4 days time, and the pine wood nematode that is present in wooden packing material Zhong mostly is greatly 3 instar larvaes of resting state, Zhi will can abolish the pine wood nematode dormancy stage of larvae and the growth conditions of optimum is provided, obtaining in the time that just but Zai is very short realized the fast detecting to the wooden packing pine wood nematode for the adult of identifying. Yu is that abolishing the pine wood nematode dormancy stage of larvae is the key that realizes rapid quarantine.
Summary of the invention
Purpose of the present invention just provides and a kind ofly dormancy pine wood nematode You worm shot up be adult, the rapid inspection for nematode larva of dormant pine wood of being convenient to detect.
Rapid inspection for nematode larva of dormant pine wood of the present invention is:
1, wooden packing or timber are split into fritter;
2, the broken material Yong of splitting of required detection is bound up with gauze, put into the container that fills culture solution;
3, will fill separation cultivation under the 20-41 ℃ of condition of container Zhi Yu of splitting broken material;
4, nutrient solution Yong 100 orders and 500 order mesh screens filter, and 100 purposes can be enclosed within above the 500 order mesh screens;
5, the Yong wash bottle is washed gently 500 order mesh screens and is collected nematode;
6, flushing liquor biomicroscope microscopy, and record observed result.
Described culture solution is as base fluid take hydrogen peroxide, and one or more Zu of nutrient solution, gibberellin, potassium nitrate, clenobuterol hydrochloride Zhong close in addition, wherein nutrient solution is dehydrogenation Cong acid and rare mixture that comes into leaves, and the concentration of used hydrogen peroxide is 1 * 10-7-9×10
-3, gibberellin concentration is 1-1000ppm, the dilute concentration that comes into leaves is 1.67 * 10-8-5×10
-2, dehydrogenation Cong acid concentration is 1.67 * 10-8-5×10
-2, clenobuterol hydrochloride concentration is 1ppt-1000ppb, potassium nitrate concentration is 1.1 * 10-5-2.2×10
-2。
Rapid inspection for nematode larva of dormant pine wood main feature of the present invention is to make the pine wood nematode You worm fast transition of resting state for the adult of identifying, it can on average shift to an earlier date 3-4 days than conventional method and detect the dormancy pine wood nematode. You Yu Zai training oxygen liquid Zhong has added hydrogen peroxide and has been suitable for the material of pine wood nematode growth, can greatly improve the recall rate of pine wood nematode. The method is easy to operate, cost is low, good reproducibility, and data are reliable, and the inspection and quarantine mechanism that is applicable to pass in and out both at home and abroad, forest plants quarantine focal pointe, collective are to the inspection and quarantine of Wooden package, timber pine wood nematode.
The specific embodiment
Embodiment 1:
A kind of rapid inspection for nematode larva of dormant pine wood is: 1, wooden packing or timber are split into 0.5 * 15cm fritter; 2, required detection split two-layer the binding up with gauze of broken material Yong, put into the container that fills culture solution; 3, cultivate filling to separate under 25-30 ℃ of environment of container Zhi Yu of splitting broken material; 4, nutrient solution Yong 100 purpose mesh screens filter above being enclosed within 500 order mesh screens; 5, the nematode of collecting on the 500 order mesh screens is washed in the Yong wash bottle gently; 6, flushing liquor biomicroscope microscopy, and record observed result. Used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1-1000ppm gibberellin, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-8-5×10
-2It is rare to come into leaves.
Embodiment 2:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1-1000ppm gibberellin.
Embodiment 3:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1ppt-1000ppb clenobuterol hydrochloride, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-8-5×10
-2It is rare to come into leaves.
Embodiment 4:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1ppt-1000ppb clenobuterol hydrochloride.
Embodiment 5:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1.1 * 10-5-2.2×10
-2Potassium nitrate, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-8-5×10
-2It is rare to come into leaves.
Embodiment 6:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1.1 * 10-5-2.2×10
-2Potassium nitrate.
Embodiment 7:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-7-5×10
-2It is rare to come into leaves.
Embodiment 8:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1-1000ppm gibberellin, 1.1 * 10-5-2.2×10
-2Potassium nitrate.
Embodiment 9:
A kind of rapid inspection for nematode larva of dormant pine wood is identical Yu embodiment 1, but used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1ppt-1000ppb clenobuterol hydrochloride, 1.1 * 10-5-2.2×10
-2Potassium nitrate.
Following Yong experimental data is illustrated:
Conventional treatment Yu test Processing Test is statistical form as a result
| Time | Process | Conversion ratio (%) | Nematode population/head | Adult quantity/head | Process | Conversion ratio (%) | Nematode population/head | Adult quantity/head | In advance (my god) nematode Zeng/adult Zeng/head |
| 2003.1 2.9-15 | Conventional treatment | 144h7.14 | 28 | 2 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h16.67 144h60.4 | 129 | 19 | 5 antenna Zeng 101/144h become Zeng 17/144h |
| 2003.1 2.29-04 .1.3 | Conventional treatment | 120h0 | 24h7 120h36 | 0 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h16.67 120h100. 94 | 24h24 120h15 8 | 24h4 120h33 | Nematode Zeng 122/120h adult Zeng 33/120h more than 5 days |
| 2004.1. 1-7 40-25 | CK | 72h12.5 120h12.5 | 24h4 7 120h24 | 72h1 120h1 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h15.63 120h108. 06 | 24h32 120h30 4 | 24h5 120h66 | 2 days nematode Zeng 280/120h adult Zeng 65/120h |
| 2004.1. 3-9 40-25 | CK | 72h12.5 144h12.5 | 24h4 144h39 | 72h1 144h1 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h3.77 144h115. 32 | 24h53 144h40 2 | 24h2 144h65 | 2 days nematode Zeng 363/144h adult Zeng/64/144h |
| 2004.1. 13-19 | Conventional treatment | 144h50 | 24h11 144h32 | 144h1 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h20.00 144h189. 04 | 24h12 144h15 4 | 24h1 144h17 | 5 days nematode Zeng 122/144h adult Zeng 16/144h |
| 2003.3. 15-21 | Conventional treatment | 72h4.82 120h26.73 | 24h77 120h306 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h2.0 120h45.0 0 | 24h50 120h68 8 | 1 | 2 days nematode Zeng/382/120h adult Zeng/ | |
| 2004.3. 23-29 | CK | 24h2.44 144h9.27 | 24h41 144h129 | 24h1 144h3 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h5.00 144h41.7 0 | 24h80 144h38 | 24h4 144h33 | Nematode Zeng 254/144h adult Zeng 30/144h |
| 2004.3. 29-4.3 | Conventional treatment | 48h3.64 144h3.64 | 24h64 96h185 144h197 | 96h2 144h2 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h2.8 144h43.7 9 | 24h98 96h604 | 24h3 96h41 | 3 days nematode Zeng 419/96h adult Zeng/39/96h |
| 2004.4. 19-24 | Conventional treatment | 96h82.13 | 96h135 | 96h27 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h5.41 96h127.5 6 | 24h148 96h778 | 24h8 96h144 | 3 days nematode Zeng 643/96h adult Zeng 117/96h |
| Time | Process | Conversion ratio (%) | Nematode population/ | Adult quantity/ | Process | Conversion ratio (%) | Nematode population/ | Adult quantity/head | In advance (my god) nematode Zeng/head |
| Head | Head | Head | Adult Zeng/head | ||||||
| 2004.4.2 2-27 | Conventional treatment | 72h33.33 108h63.33 | 12h7 108h60 | 72h5 108h6.5 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 12h8.57 108h235 .61 | 12h18 108h32 2 | 12h1.5 108h136.5 | 2.5 it nematode Zeng 262108h adult Zeng 130/108h |
| 2004.4.2 6-30 | Conventional treatment | 96h/ | 24h6 96h31 | 96h/ | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h28.0 9 96h151. 12 | 24h178 96h573 | 24h50 96h212 | Nematode Zeng 542/96h adult Zeng 212/96h more than 4 days |
| 2004.5.2- 6 | Conventional treatment | 48h3.45 96h3.45 | 15h10 96h78 | 48h1 96h1 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 15h7.7 96h30.6 5 | 15h13 96h232 | 15h1 96h13 | 1.5 it nematode Zeng 192/96h adult Zeng 12/96h |
| 2004.5.5- 8 | Conventional treatment | 72h/ | 24h11 72h40 | 72h/ | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h18.7 5 72h29.2 8 | 24h115 72h257 | 24h3 72h5 | 2 days nematode Zeng 217/72h adult Zeng 5/72h |
| 2004.5.2 4-28 | CK | 96h5.56 | 24h92 96h242 | 96h3 | Clenobuterol hydrochloride variable concentrations, temperature | 24h0.56 96h6.31 | 24h178 96h659 | 24h1 96h9 | 3 days nematode Zeng 417/96h adult Zeng 6/96h |
| 2004.5.2 4-28 | CK | 96h5.66 | 24h92 96h242 | 96h3 | Clenobuterol hydrochloride+dioxygen variable concentrations, temperature | 48h0.4 96h10.2 5 | 24h63 96h880 | 48h1 96h22 | 3 days nematode Zeng 638/96h adult Zeng 19/96h |
| 2004.6.2- 6 | Conventional treatment | 96h/ | 24h4 96h18 | 96h/ | Clenobuterol hydrochloride+rare+dehydrogenation Cong the acid of coming into leaves, variable concentrations, temperature | 24h38.4 6 96h108. 06 | 24h15 96h161 | 24h5 96h27 | Nematode Zeng 143/96h adult Zeng 27/96h more than 4 days |
| 200.4.6.2 9-7.3 | Conventional treatment | 48h9.52 72h17.71 | 24h26 72h101 | 72h10 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h12.9 72h55.6 3 | 24h82 72h184 | 24h15 72h29 | 2 days nematode Zeng 83/72h adult Zeng 19/72h |
| 2004.7.6- 12 | Conventional treatment | 144h11.42 | 24h108 144h277 | 144h4 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h0.46 144h24. 39 | 24h560 144h17 17 | 24h1 144h42 | 5 days nematode Zeng 1440/144h adult Zeng 38/144h |
| 2004.7.1 9-23 | Conventional treatment | 96h/ | 24h55 96h214 | 96h/ | Hydrogen peroxide+rare+dehydrogenation Cong acid the hydrogen peroxide that comes into leaves+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h6.25 96h11.5 | 24h147 96h649 | 24h1 96h12 | 4 days nematode Zeng 435/96h adult Zeng 12/96h |
| Time | Process | Conversion ratio (%) | Nematode population/head | Adult quantity/head | Process | Conversion ratio (%) | Nematode population/head | Adult quantity/head | In advance (my god) nematode Zeng/adult Zeng/head |
| 2004.7.24-8.2 | Conventional treatment | 216h18.75 | 24h19 216h141 | 216h3 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 48h1.5 216h10*. | 24h59 216h37 0 | 48h1 216h37 | 8 days nematode Zeng 229/216h adult Zeng 34/216h |
| 2004.7.26-30 | Conventional treatment | 96h20. | 24h1 96h38 | 96h1 | Hydrogen peroxide+rare+dehydrogenation Cong acid+clenobuterol hydrochloride variable concentrations, temperature come into leaves | 24h7.69 96h18.80 | 24h30 96h155 | 24h2 96h14 | 3 days nematode Zeng 117/96h adult Zeng 13/96h |
| 2004.8.2-6 | Conventional treatment | 96/ | 24h27 96h69 | 96h/ | Clenobuterol hydrochloride variable concentrations, temperature | 24h4.44 96h40.59 | 24h89 96h324 | 24h2 96h19 | Nematode Zeng 255/96h adult Zeng 19/96h more than 4 days |
| 2004.8.9-13 | Conventional treatment | 96h25 360h/0 | 24h25 96h61 360h25 | 360h/0 | Clenobuterol hydrochloride variable concentrations, temperature | 24h3.3 96h41.15 | 24h148 96h579 | 24h1 96h25 | 3 days nematode Zeng 518/96h adult Zeng 25/96h |
| 2004.8.16-20 ck253 Zai 192 ready-made worms | Conventional treatment) | 72h1.78 96h1.78(2) | 24h125 96h448 | 72h1 96h1 | Clenobuterol hydrochloride variable concentrations, temperature | 24h0.62 96h30.41 | 24h302 96h134 | 24h1 96h65 | 2 days nematode Zeng 899/96h adult Zeng 64/96h |
| 2004.8.23-27 | Conventional treatment | The 96h/ 168h ready-made worm that begins. | 24h43 96h215 | 96h/ | Clenobuterol hydrochloride variable concentrations, temperature | 24h2.9 96h18.02 | 24h131 96h637 | 24h2 96h20 | 11 days nematode Zeng 422/96h adult Zeng 20/96h |
| 2004.8.9-9.3 | Conventional treatment | 96h/ | 24h7 96h76 | 96h/ | Clenobuterol hydrochloride+potassium nitrate variable concentrations, temperature | 24h1.96 96h28.02 | 24h76 96h412 | 24h1 96h15 | Nematode Zeng 336/96h adult Zeng 15/96h more than 4 days |
| 2004.9.20-24 | Conventional treatment | 96h/ 120h1 | 24h10 96h82 | 96h/ | Gibberellin+hydrogen peroxide variable concentrations, | 24h33.33 96h110.98 | 24h17 96h174 | 24h1 96h34 | 4-9 days nematode Zeng 92/96h |
| 240h1 360h32.47 | 360h173 | 360h9 | Temperature | Adult Zeng 34/96h | |||||
| 2004.9.29-10.3 | Conventional treatment | 96h16.67 240h3 360h4 | 24h20 96h39 36067 | 96h1 360h4 | Gibberellin+hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h12.5 96h129.16 | 24h13 96h101 | 24h1 96h18 | 3 days nematode Zeng 62/96h adult Zeng 17/96h |
| 2004.10.3-8 | Conventional treatment | 120h/ 264h33.33 | 24h6 120h27 264h40 | 120h/ 264h1 | Gibberellin+hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h20.00 120h105.7 1 | 24h4 120h81 | 24h1 120h15 | 10 days nematode Zeng 54/120h adult Zeng 15/120h |
| Time | Process | Conversion ratio (%) | Nematode population/head | Adult quantity/head | Process | Conversion ratio (%) | Nematode population/head | Adult quantity/head | In advance (my god) nematode Zeng/adult Zeng/head |
| 2004.10.13-18 | Conventional treatment | 240h/ | 24h1 240h20 | 240h/ | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 24h8.33 240h536.0 4 | 24h11 240h21 3 | 24h1 240h61 | Nematode Zeng 193/240h adult Zeng 61/240h more than 10 days |
| 2004.10.20-25 | Conventional treatment | 96h/ 120h begins existing 264 | 24h3 96h23 264h34 | 96h/ 120h1 264h2 | Gibberellin+hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 48h15.00 96h166.85 | 24h2 96h67 | 48h3 96h37 | 4 days nematode Zeng 44/96h adult Zeng 37/96h |
| 2004.10.25-29 | Conventional treatment | 168h/ | 24h3 168h18 | 168h/ | Gibberellin+hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature, temperature come into leaves | 48h14.29 168h124.2 9 | 24h33 168h16 | 48h1 168h11 | Nematode Zeng 144/168/ adult Zeng 11/168h more than 7 days |
| 2004.11.22-26 | Conventional treatment | 96h/ | 24h3 96h10 | 96h/ | Hydrogen peroxide+rare+dehydrogenation Cong acid+potassium nitrate variable concentrations, temperature come into leaves | 24h2.7 | 24h47 96h126 | 24h1 96h20 | Nematode Zeng 116/96h adult Zeng 20/96h more than 4 days |
| 2004.12.14-18 | Conventional treatment | 96h25 26457.5 | 24h9 96h24 264h46 | 96h1 264h3 | Hydrogen peroxide+rare+dehydrogenation Cong acid variable concentrations, temperature come into leaves | 48h1613 96h62.56 | 24h19 96h84 | 48h5 96h18 | 3 days nematode Zeng 60/96h adult Zeng 17/96h |
Claims (10)
1, a kind of rapid inspection for nematode larva of dormant pine wood, it is characterized in that: it may further comprise the steps:
(1) wooden packing or timber are split into fritter; (2) the broken material Yong that splits of required detection binds up with gauze, and puts into the container that fills culture solution; (3) will fill separation cultivation under 20-41 ℃ of environment of container Zhi Yu of splitting broken material; (4) nutrient solution Yong 100 orders and 500 order mesh screens are filtered, 100 purpose mesh screens can be enclosed within above the 500 order mesh screens; (5) nematode of collecting on the 500 order mesh screens is washed in the Yong wash bottle gently; (6) flushing liquor liquid biomicroscope microscopy, the record observed result;
Described culture solution is take hydrogen peroxide as base fluid, and in addition one or more Zu of nutrient solution, gibberellin, potassium nitrate, clenobuterol hydrochloride Zhong close, and wherein nutrient solution is dehydrogenation Cong acid and rare mixture that comes into leaves; The concentration of used hydrogen peroxide is 1 * 10-7-9×10
-3, gibberellin concentration is 1-1000ppm, the dilute concentration that comes into leaves is 1.67 * 10-8-5×10
-2, dehydrogenation Cong acid concentration is 1.67 * 10-8-5×10
-2, clenobuterol hydrochloride concentration is 1ppt-1000ppb, potassium nitrate concentration is 1.1 * 10-5-2.2×10
-2。
2, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1-1000ppm gibberellin, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-8-5×10
-2It is rare to come into leaves.
3, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1-1000ppm gibberellin.
4, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1ppt-1000ppb clenobuterol hydrochloride, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-8-5×10
-2It is rare to come into leaves.
5, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1ppt-1000ppb clenobuterol hydrochloride.
6, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1.1 * 10-5-2.2×10
-2Potassium nitrate, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-8-5×10
-2It is rare to come into leaves.
7, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1ppt-1000ppb clenobuterol hydrochloride, 1.1 * 10-5-2.2×10
-2Potassium nitrate.
8, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1.67 * 10-8-5×10
-2Dehydrogenation Cong acid, 1.67 * 10-7-5×10
-2It is rare to come into leaves.
9, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1-1000ppm gibberellin, 1.1 * 10-5-2.2×10
-2Potassium nitrate.
10, rapid inspection for nematode larva of dormant pine wood according to claim 1 is characterized in that: used culture solution comprises 1 * 10-7-9×10
-3Hydrogen peroxide, 1.1 * 10-5-2.2×10
-2Potassium nitrate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510018185 CN1645135B (en) | 2005-01-21 | 2005-01-21 | Quarantine method for nematode larva of dormant pine wood |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510018185 CN1645135B (en) | 2005-01-21 | 2005-01-21 | Quarantine method for nematode larva of dormant pine wood |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1645135A true CN1645135A (en) | 2005-07-27 |
| CN1645135B CN1645135B (en) | 2010-09-08 |
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| CN 200510018185 Expired - Fee Related CN1645135B (en) | 2005-01-21 | 2005-01-21 | Quarantine method for nematode larva of dormant pine wood |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101149366A (en) * | 2007-10-16 | 2008-03-26 | 李红玉 | Method for quickly quantifying eelworm death rate |
| CN101832889A (en) * | 2010-04-30 | 2010-09-15 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN102965271A (en) * | 2012-11-07 | 2013-03-13 | 苏州市外来有害生物防控技术中心 | Device for quickly extracting pine wood nematode nucleic acid |
| CN104412954A (en) * | 2013-08-22 | 2015-03-18 | 天津卫技天马除害处理技术服务有限公司 | Quarantine treatment system and method for nematodes among flower and plant seed balls |
| CN105432566A (en) * | 2015-10-30 | 2016-03-30 | 泸县农林局 | Method for rapidly detecting pine wood nematodes |
| CN115176559A (en) * | 2022-07-21 | 2022-10-14 | 上海海关动植物与食品检验检疫技术中心 | Method for separating lethal granular nematodes |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167806C (en) * | 2002-08-23 | 2004-09-22 | 厦门大学 | Method for early diagnosis and detection of pine nematode disease |
| CN1184479C (en) * | 2003-01-21 | 2005-01-12 | 厦门大学 | Fast separation device of pine material nematode and its detection method |
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- 2005-01-21 CN CN 200510018185 patent/CN1645135B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101149366A (en) * | 2007-10-16 | 2008-03-26 | 李红玉 | Method for quickly quantifying eelworm death rate |
| CN101149366B (en) * | 2007-10-16 | 2015-03-04 | 李红玉 | Method for quickly quantifying eelworm death rate |
| CN101832889A (en) * | 2010-04-30 | 2010-09-15 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN101832889B (en) * | 2010-04-30 | 2011-11-23 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN102965271A (en) * | 2012-11-07 | 2013-03-13 | 苏州市外来有害生物防控技术中心 | Device for quickly extracting pine wood nematode nucleic acid |
| CN102965271B (en) * | 2012-11-07 | 2014-05-28 | 苏州市外来有害生物防控技术中心 | Device for quickly extracting pine wood nematode nucleic acid |
| CN104412954A (en) * | 2013-08-22 | 2015-03-18 | 天津卫技天马除害处理技术服务有限公司 | Quarantine treatment system and method for nematodes among flower and plant seed balls |
| CN105432566A (en) * | 2015-10-30 | 2016-03-30 | 泸县农林局 | Method for rapidly detecting pine wood nematodes |
| CN105432566B (en) * | 2015-10-30 | 2018-10-26 | 泸县农林局 | A kind of method of quick detection Bursaphelenchus xylophilus |
| CN115176559A (en) * | 2022-07-21 | 2022-10-14 | 上海海关动植物与食品检验检疫技术中心 | Method for separating lethal granular nematodes |
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