CN1184479C - Fast separation device of pine material nematode and its detection method - Google Patents
Fast separation device of pine material nematode and its detection method Download PDFInfo
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- CN1184479C CN1184479C CN 03103072 CN03103072A CN1184479C CN 1184479 C CN1184479 C CN 1184479C CN 03103072 CN03103072 CN 03103072 CN 03103072 A CN03103072 A CN 03103072A CN 1184479 C CN1184479 C CN 1184479C
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Abstract
The present invention relates to a device for separating pine wood nematode and a detection method. The device comprises a pumping filter cylinder, a sleeve cylinder and a vacuum pump, wherein the pumping filter cylinder is provided with a tube body, the bottom of which is closed; a small hole and a pipeline are fixed on the tube wall of the pumping filter cylinder; the sleeve cylinder is a hollow tube body; the vacuum pump is connected with a pipeline of the pumping filter cylinder by a pressure tube. The pumping filter cylinder and the sleeve cylinder are relatively embedded on a pine which is to be inspected; water is poured in the sleeve cylinder; the water is pumped and filtered in the pumping filter cylinder by the vacuum pump; then, the nematode and kinds of the nematode are inspected in underwater deposition by an anatomical lens and a high power microscope. The hidden nematode is separated from the pine by the method of the present invention, and the original separation work which needs more than 24 hours to finish is completed within 20 to 30 minutes in order to identify the pine wood nematode at a high speed; therefore, the efficiency of quarantine and examination work is enhanced, wood products and goods which are packed by the wood can quickly pass through toll-gates, and at the same time, the trouble of the quarantine and inspection personnel that the wood is cut into sheets is relieved.
Description
(1) technical field
The present invention relates to a kind of fast separation device and the detection method thereof of pine nematode pathogenic nematode.
(2) background technology
Pine wood nematode (Bursaphelenchus xylophilus) causes pine tree wilt disease, is called as " smokeless forest fire " or " pine tree cancer ".Because the pine wood nematode reproduction speed is fast, and by insect-borne transmissions such as longicorns, causes disaster so spread very soon, make large stretch of pine tree dead rapidly.Therefore, forbid that it is one of major measure that prevents this disease diffusion that lesion timber moves.In addition, the Wooden packing container of cargoes imported and exported much is a pine, also is to cause this sick main cause.For preventing that this disease from further spreading, the inspection post is set up on main traffic main artery, China various places at present, interception pine tree diseased wood; The Wooden packing container of cargoes imported and exported is also tightly checked at each port.
But, from timber, isolate nematode and also identified, be a time-consuming loaded down with trivial details job of taking a lot of work.Usually must be split into little (also thinner) earlier after the timber sampling, be placed on then in the containers such as funnel or large beaker, separate, generally need just can isolate nematode through 24 hours by the graceful funnel ratio juris of Bel than chopsticks.(the sick worm of Chinese forest such as Jian Yongxun, 2001, the 4th phase, 5-6,23) Bao Dao " research of pine wood nematode quarantine and examination sampling method ", though it is more laborsaving than splitting head to adopt electric drill to drill through wood chip, separation method is not broken away from " the graceful funnel of Bel ", therefore still needs just can go out the result more than 20 hours.And in each inspection post, the owner of cargo and automobilist are extremely unwilling suffering on the outpost of the tax office time more than a day, and that means enormous economic loss.Port quarantine is just bigger because of the loss of waiting for out the result and causing.So the quick test of pine wood nematode is needing to be resolved hurrily of a task, therefore, " eight or five ", the enforcement period of the ninth five-year plan, the tackling key problem of once setting up the project of China forest department, drop on the wood though work out with chemical reagent, judge according to color change then to have or not nematode (Wang Yu Yan etc. " two kinds of indicator are to the test of pine nematode diseased wood ", the 4th national plant nematology seminar world-of-mouth communication material), but require not to be used because of sensitivity reaches.
(3) summary of the invention
Purpose of the present invention aims to provide a kind of principle of utilizing pressure reduction, makes the nematode in the timber be taken out of tripping device and the detection method of being identified thereof smoothly by the current that pass through rapidly.
The said tripping device of the present invention comprises suction filtration tube, sleeve and vacuum pump.The suction filtration tube is the body of a base seal, and diameter is 4～10cm, and height is 10～20cm, establishes an aperture on the tube wall of suction filtration tube, and sets firmly a pipeline at aperture; Sleeve is a hollow tube, and height is 5～15cm; Vacuum pump is connected by the pipeline of pressure pipe with the suction filtration tube.
Suction filtration tube and sleeve adopt metal, glass or engineering plastics to make.
One port of the nozzle of suction filtration tube and sleeve is knife edge shape edge.
Aperture on the suction filtration pipe wall preferably is located at the middle and upper part of suction filtration tube.The diameter of sleeve is preferably identical or close with the diameter of suction filtration tube.
The said detection method of the present invention is as follows:
Saw is got one section diameter 〉=suction filtration tube diameter, is about the pine to be checked of 3～6cm;
One end of the nozzle of suction filtration tube and sleeve relatively is embedded on the xsect of pine to be checked;
The pine that is embedded with sleeve is faced up, clear water is poured in the sleeve;
Start vacuum pump, the water suction filtration in the sleeve is gone in the suction filtration tube of the pine other end;
Close the power supply of vacuum pump, take off the suction filtration tube, the water in the tube is poured in the double dish, precipitate 1～2 time, then the sediment in the double dish is checked the kind of nematode and nematode under anatomical lens and high-power microscope with clear water from pine.
In addition, moisture enters vacuum pump when preventing suction filtration, can be connected a bottle,suction that drying agent is housed between vacuum pump and suction filtration tube.
In pine, isolate hiding nematode with this method of the present invention, make the mask work of original need more than 24 hours be able within 20～30 minutes, finish, be convenient to the Rapid identification pine wood nematode, improve the efficient of quarantine and examination work, accelerated the card speed of being open to the custom of Wood products and wooden packing goods greatly.Also exempted simultaneously the hardship that quarantine and examination personnel splitting is chipped.
(4) description of drawings
Fig. 1 is the structural representation of tripping device.
(5) embodiment
The invention will be further described below in conjunction with embodiment.
As shown in Figure 1, suction filtration tube 1 is 5.5 centimetres of internal diameters, 6 centimetres of external diameters, high 18 centimetres galvanized conduit, the lower port of suction filtration tube makes base seal through welding an iron plate, get out an aperture and a bit of tubule road 11 of burn-oning on the tube wall at 2/3 place, bottom, the diameter in tubule road is advisable can be inserted in pressure pipe (plastics or hard proofed sleeve).Pressure pipe links to each other with vacuum pump, and the last nozzle of suction filtration tube is ground to knife edge shape edge 12; The diameter of sleeve 2 is with the suction filtration tube, and is high 8.5 centimetres, and a port similarly is ground to knife edge shape edge 21.
Moisture enters vacuum pump when preventing suction filtration, can be connected a bottle,suction that silica gel is housed between vacuum pump and suction filtration tube.
From about 3～6 centimetres juggle of pine saw one segment length, its diameter is about 7 centimetres.Upper edge and the sleeve sharp keen lower edge sharp keen the suction filtration tube embed on the xsect of pine section to be checked, both sharp keen edge directions are relative, the degree of depth that embeds xsect is about 0.2～0.5cm, pine section to be checked tightly is stuck between surface sleeve and the following suction filtration tube, subsequently in the sleeve above the clear water impouring.Put appropriate after, start vacuum pump, in the sleeve clear water of hundreds of milliliter in minutes just by the pine section, all " filter " go in the following suction filtration tube.
The power supply of turning off vacuum pump is extracted the pine section, and the water in the suction filtration tube is poured in the double dish, after adding clear water precipitation 2 times, sediment in the double dish is placed under the anatomical lens checks and have or not nematode,, just can allow the clearance of timber and delivery vehicle thereof if what nematode does not all have; If nematode is arranged, then draw several nematodes, be contained in covered on the microslide, be placed on and identify it is what nematode under the high-power microscope, if pine wood nematode is arranged, must handle, or destroy by the relevant regulations of country, or fumigation.
Claims (6)
1. the fast separation device of pine wood nematode is characterized in that comprising suction filtration tube, sleeve and vacuum pump; The suction filtration tube is the body of a base seal, and diameter is 4～10cm, and height is 10～20cm, establishes an aperture on the tube wall of suction filtration tube, and sets firmly a pipeline at aperture; Sleeve is a hollow tube, and height is 5～15cm; Vacuum pump is connected by the pipeline of pressure pipe with the suction filtration tube.
2. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that the last nozzle of suction filtration tube is knife edge shape edge.
3. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that a port of sleeve is knife edge shape edge.
4. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that suction filtration tube and sleeve adopt metal, glass or engineering plastics to make.
5. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that being connected a bottle,suction that drying agent is housed between vacuum pump and suction filtration tube.
6. the detection method of pine wood nematode is characterized in that said detection method is as follows:
1) saw is got one section diameter 〉=suction filtration tube diameter, is about the pine to be checked of 3～6cm;
2) end of the nozzle of suction filtration tube and sleeve relatively is embedded on the xsect of pine to be checked;
3) pine that will be embedded with sleeve faces up, and clear water is poured in the sleeve;
4) start vacuum pump, the water suction filtration in the sleeve is gone in the suction filtration tube of the pine other end;
5) close the power supply of vacuum pump, take off the suction filtration tube, the water in the tube is poured in the double dish,, then the sediment in the double dish is checked the kind of nematode and nematode under anatomical lens and high-power microscope with clear water precipitation 1～2 time from pine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03103072 CN1184479C (en) | 2003-01-21 | 2003-01-21 | Fast separation device of pine material nematode and its detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03103072 CN1184479C (en) | 2003-01-21 | 2003-01-21 | Fast separation device of pine material nematode and its detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1430061A CN1430061A (en) | 2003-07-16 |
| CN1184479C true CN1184479C (en) | 2005-01-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03103072 Expired - Fee Related CN1184479C (en) | 2003-01-21 | 2003-01-21 | Fast separation device of pine material nematode and its detection method |
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| CN (1) | CN1184479C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397062C (en) * | 2005-08-01 | 2008-06-25 | 厦门大学 | Apparatus for quick separation of pine wood nematode and detection method therefor |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1645135B (en) * | 2005-01-21 | 2010-09-08 | 江西出入境检验检疫局检验检疫综合技术中心 | Quarantine method for nematode larva of dormant pine wood |
| CN100363729C (en) * | 2005-07-21 | 2008-01-23 | 云南农业大学 | Portable fast separation device of pine material nematode and its separation method |
| CN100507517C (en) * | 2006-03-24 | 2009-07-01 | 中国科学院动物研究所 | Chemical dyeing method for differentiating reproduction type pine wood nematode |
| CN100504354C (en) * | 2006-03-24 | 2009-06-24 | 中国科学院动物研究所 | Chemical dyeing method for differentiating diffuse type pine wood nematode |
| CN101832889B (en) * | 2010-04-30 | 2011-11-23 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN103471901B (en) * | 2013-08-29 | 2016-05-25 | 广西大学 | A kind of sporangiocyst method that captures that separates |
| CN105432566B (en) * | 2015-10-30 | 2018-10-26 | 泸县农林局 | A kind of method of quick detection Bursaphelenchus xylophilus |
| CN107356459B (en) * | 2017-06-20 | 2020-09-15 | 潘沧桑 | Detachable and washable fast pine wood nematode separating device and detection method thereof |
-
2003
- 2003-01-21 CN CN 03103072 patent/CN1184479C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397062C (en) * | 2005-08-01 | 2008-06-25 | 厦门大学 | Apparatus for quick separation of pine wood nematode and detection method therefor |
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| CN1430061A (en) | 2003-07-16 |
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Owner name: PAN CANGSANG Free format text: FORMER OWNER: XIAMEN UNIVERSITY Effective date: 20140124 |
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Effective date of registration: 20140124 Address after: Xiamen University East Siming District of Xiamen city in Fujian province 361005 Building No. 5 Room 202 Patentee after: Pan Cangsang Address before: Xiamen City, Fujian Province, 361005 South Siming Road No. 422 Patentee before: Xiamen University |
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