CN1430061A - Fast separation device of pine material nematode and its detection method - Google Patents
Fast separation device of pine material nematode and its detection method Download PDFInfo
- Publication number
- CN1430061A CN1430061A CN 03103072 CN03103072A CN1430061A CN 1430061 A CN1430061 A CN 1430061A CN 03103072 CN03103072 CN 03103072 CN 03103072 A CN03103072 A CN 03103072A CN 1430061 A CN1430061 A CN 1430061A
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- China
- Prior art keywords
- suction filtration
- pine
- sleeve
- filtration tube
- tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000008331 Pinus X rigitaeda Nutrition 0.000 title claims abstract description 27
- 235000011613 Pinus brutia Nutrition 0.000 title claims abstract description 27
- 241000018646 Pinus brutia Species 0.000 title claims abstract description 27
- 241000244206 Nematoda Species 0.000 title claims abstract description 20
- 238000000926 separation method Methods 0.000 title claims description 9
- 238000001514 detection method Methods 0.000 title claims description 7
- 239000000463 material Substances 0.000 title description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000000967 suction filtration Methods 0.000 claims description 38
- 241000243771 Bursaphelenchus xylophilus Species 0.000 claims description 14
- 239000013049 sediment Substances 0.000 claims description 3
- 239000002274 desiccant Substances 0.000 claims description 2
- 229920006351 engineering plastic Polymers 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- 239000002184 metal Substances 0.000 claims description 2
- 229910052751 metal Inorganic materials 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 239000002023 wood Substances 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 3
- 238000005086 pumping Methods 0.000 abstract 5
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000012856 packing Methods 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 210000005239 tubule Anatomy 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003958 fumigation Methods 0.000 description 1
- 230000009357 insect borne transmission Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Landscapes
- Catching Or Destruction (AREA)
Abstract
A high-speed separator for detecting pine nematode is composed of a pumping filter barrel, a sleeve tube and a vacuum pump communicated with the tube on the wall of pumping filter barrel. Its detecting method includes such steps as embedding said pumping filter barrel and sleeve tube in pine wood to be detected, filling water in sleeve tube, pumping water in the pumping filter barrel, and detecting pine nematode by microscope. Its advantage is high speed (20-30 min).
Description
(1) technical field
The present invention relates to a kind of fast separation device and the detection method thereof of pine nematode pathogenic nematode.
(2) background technology
Pine wood nematode (Bursaphelenchus xylophilus) causes pine tree wilt disease, is called as " smokeless forest fire " or " pine tree cancer ".Because the pine wood nematode reproduction speed is fast, and by insect-borne transmissions such as longicorns, causes disaster so spread very soon, make large stretch of pine tree dead rapidly.Therefore, forbid that it is one of major measure that prevents this disease diffusion that lesion timber moves.In addition, the Wooden packing container of cargoes imported and exported much is a pine, also is to cause this sick main cause.For preventing that this disease from further spreading, the inspection post is set up on main traffic main artery, China various places at present, interception pine tree diseased wood; The Wooden packing container of cargoes imported and exported is also tightly checked at each port.
But, from timber, isolate nematode and also identified, be a time-consuming loaded down with trivial details job of taking a lot of work.Usually must be split into little (also thinner) earlier after the timber sampling, be placed on then in the containers such as funnel or large beaker, separate, generally need just can isolate nematode through 24 hours by the graceful funnel ratio juris of Bel than chopsticks.(the sick worm of Chinese forest such as Jian Yongxun, 2001, the 4th phase, 5-6,23) Bao Dao " research of pine wood nematode quarantine and examination sampling method ", though it is more laborsaving than splitting head to adopt electric drill to drill through wood chip, separation method is not broken away from " the graceful funnel of Bel ", therefore still needs just can go out the result more than 20 hours.And in each inspection post, the owner of cargo and automobilist are extremely unwilling suffering on the outpost of the tax office time more than a day, and that means enormous economic loss.Port quarantine is just bigger because of the loss of waiting for out the result and causing.So the quick test of pine wood nematode is needing to be resolved hurrily of a task, therefore, " eight or five ", the enforcement period of the ninth five-year plan, the tackling key problem of once setting up the project of China forest department, drop on the wood though work out with chemical reagent, judge according to color change then to have or not nematode (Wang Yu Yan etc. " two kinds of indicator are to the test of pine nematode diseased wood ", the 4th national plant nematology seminar world-of-mouth communication material), but require not to be used because of sensitivity reaches.
(3) summary of the invention
Purpose of the present invention aims to provide a kind of principle of utilizing pressure reduction, makes the nematode in the timber be taken out of tripping device and the detection method of being identified thereof smoothly by the current that pass through rapidly.
The said tripping device of the present invention comprises suction filtration tube, sleeve and vacuum pump.The suction filtration tube is the body of a base seal, and diameter is 4～10cm, and height is 10～20cm, establishes an aperture and set firmly a pipeline on the tube wall of suction filtration tube; Sleeve is a hollow tube, and height is 5～15cm; Vacuum pump is connected by the pipeline of pressure pipe with the suction filtration tube.
Suction filtration tube and sleeve adopt metal, and glass or engineering plastics are made.
One port of the nozzle of suction filtration tube and sleeve is knife edge shape edge.
Aperture on the suction filtration pipe wall preferably is located at the middle and upper part of suction filtration tube.The diameter of sleeve is preferably identical or close with the diameter of suction filtration tube.
The said detection method of the present invention is as follows:
Saw is got the pine to be checked that one section diameter is not less than the suction filtration tube, is about 3～6cm;
One end of the nozzle of suction filtration tube and sleeve relatively is embedded on the xsect of pine to be checked;
The pine that is embedded with sleeve is faced up, clear water is poured in the sleeve;
Start vacuum pump, the water suction filtration in the sleeve is gone in the suction filtration tube of the pine other end;
Close the power supply of vacuum pump, take off the suction filtration tube, the water in the tube is poured in the double dish, precipitate 1～2 time, then the sediment in the double dish is checked the kind of nematode and nematode under anatomical lens and high-power microscope with clear water from pine.
In addition, moisture enters vacuum pump when preventing suction filtration, can be connected a bottle,suction that drying agent is housed between vacuum pump and suction filtration tube.
In pine, isolate hiding nematode with this method of the present invention, make the mask work of original need more than 24 hours be able within 20～30 minutes, finish, be convenient to the Rapid identification pine wood nematode, improve the efficient of quarantine and examination work, accelerated the card speed of being open to the custom of Wood products and wooden packing goods greatly.Also exempted simultaneously the hardship that quarantine and examination personnel splitting is chipped.
(4) description of drawings
Fig. 1 is the structural representation of tripping device.
(5) embodiment
The invention will be further described below in conjunction with embodiment.
As shown in Figure 1, suction filtration tube 1 is 5.5 centimetres of internal diameters, 6 centimetres of external diameters, high 18 centimetres galvanized conduit, the lower port of suction filtration tube makes base seal through welding an iron plate, get out an aperture and a bit of tubule road 11 of burn-oning on the tube wall at 2/3 place, bottom, the diameter in tubule road is advisable can be inserted in pressure pipe (plastics or hard proofed sleeve).Pressure pipe links to each other with vacuum pump, and the last nozzle of suction filtration tube is ground to knife edge shape edge 12; The diameter of sleeve 2 is with the suction filtration tube, and is high 8.5 centimetres, and a port similarly is ground to knife edge shape edge 21.
Moisture enters vacuum pump when preventing suction filtration, can be connected a bottle,suction that silica gel is housed between vacuum pump and suction filtration tube.
From about 3～6 centimetres juggle of pine saw one segment length, its diameter is about 7 centimetres.Upper edge and the sleeve sharp keen lower edge sharp keen the suction filtration tube embed on the xsect of pine section to be checked, both sharp keen edge directions are relative, the degree of depth that embeds xsect is about 0.2～0.5cm, pine section to be checked tightly is stuck between surface sleeve and the following suction filtration tube, subsequently in the sleeve above the clear water impouring.Put appropriate after, start vacuum pump, in the sleeve clear water of hundreds of milliliter in minutes just by the pine section, all " filter " go in the following suction filtration tube.
The power supply of turning off vacuum pump is extracted the pine section, and the water in the suction filtration tube is poured in the double dish, after adding clear water precipitation 2 times, sediment in the double dish is placed under the anatomical lens checks and have or not nematode,, just can allow the clearance of timber and delivery vehicle thereof if what nematode does not all have; If nematode is arranged, then draw several nematodes, be contained in covered on the microslide, be placed on and identify it is what nematode under the high-power microscope, if pine wood nematode is arranged, must handle, or destroy by the relevant regulations of country, or fumigation.
Claims (6)
1. the fast separation device of pine wood nematode is characterized in that comprising suction filtration tube, sleeve and vacuum pump; The suction filtration tube is the body of a base seal, and diameter is 4～10cm, and height is 10～20cm, establishes an aperture and set firmly a pipeline on the tube wall of suction filtration tube; Sleeve is a hollow tube, and height is 5～15cm; Vacuum pump is connected by the pipeline of pressure pipe with the suction filtration tube.
2. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that the last nozzle of suction filtration tube is knife edge shape edge.
3. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that a port of sleeve is knife edge shape edge.
4. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that suction filtration tube and sleeve adopt metal, and glass or engineering plastics are made.
5. the fast separation device of pine wood nematode as claimed in claim 1 is characterized in that being connected a bottle,suction that drying agent is housed between vacuum pump and suction filtration tube.
6. the detection method of pine wood nematode is characterized in that said detection method is as follows:
1) saw is got the pine to be checked that one section diameter is not less than the suction filtration tube, is about 3～6cm;
2) end of the nozzle of suction filtration tube and sleeve relatively is embedded on the xsect of pine to be checked;
3) pine that will be embedded with sleeve faces up, and clear water is poured in the sleeve;
4) start vacuum pump, the water suction filtration in the sleeve is gone in the suction filtration tube of the pine other end;
5) close the power supply of vacuum pump, take off the suction filtration tube, the water in the tube is poured in the double dish,, then the sediment in the double dish is checked the kind of nematode and nematode under anatomical lens and high-power microscope with clear water precipitation 1～2 time from pine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03103072 CN1184479C (en) | 2003-01-21 | 2003-01-21 | Fast separation device of pine material nematode and its detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 03103072 CN1184479C (en) | 2003-01-21 | 2003-01-21 | Fast separation device of pine material nematode and its detection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1430061A true CN1430061A (en) | 2003-07-16 |
| CN1184479C CN1184479C (en) | 2005-01-12 |
Family
ID=4790038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 03103072 Expired - Fee Related CN1184479C (en) | 2003-01-21 | 2003-01-21 | Fast separation device of pine material nematode and its detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1184479C (en) |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100363729C (en) * | 2005-07-21 | 2008-01-23 | 云南农业大学 | Portable fast separation device of pine material nematode and its separation method |
| CN100504354C (en) * | 2006-03-24 | 2009-06-24 | 中国科学院动物研究所 | Chemical dyeing method for differentiating diffuse type pine wood nematode |
| CN100507517C (en) * | 2006-03-24 | 2009-07-01 | 中国科学院动物研究所 | Chemical dyeing method for differentiating reproduction type pine wood nematode |
| CN1645135B (en) * | 2005-01-21 | 2010-09-08 | 江西出入境检验检疫局检验检疫综合技术中心 | Quarantine method for nematode larva of dormant pine wood |
| CN101832889A (en) * | 2010-04-30 | 2010-09-15 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN103471901A (en) * | 2013-08-29 | 2013-12-25 | 广西大学 | Sporocyst separating and grabbing tool |
| CN105432566A (en) * | 2015-10-30 | 2016-03-30 | 泸县农林局 | Method for rapidly detecting pine wood nematodes |
| CN107356459A (en) * | 2017-06-20 | 2017-11-17 | 潘沧桑 | The fast separation device of pine material nematode and its detection method of disassembling cleaning |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100397062C (en) * | 2005-08-01 | 2008-06-25 | 厦门大学 | Apparatus for quick separation of pine wood nematode and detection method therefor |
-
2003
- 2003-01-21 CN CN 03103072 patent/CN1184479C/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1645135B (en) * | 2005-01-21 | 2010-09-08 | 江西出入境检验检疫局检验检疫综合技术中心 | Quarantine method for nematode larva of dormant pine wood |
| CN100363729C (en) * | 2005-07-21 | 2008-01-23 | 云南农业大学 | Portable fast separation device of pine material nematode and its separation method |
| CN100504354C (en) * | 2006-03-24 | 2009-06-24 | 中国科学院动物研究所 | Chemical dyeing method for differentiating diffuse type pine wood nematode |
| CN100507517C (en) * | 2006-03-24 | 2009-07-01 | 中国科学院动物研究所 | Chemical dyeing method for differentiating reproduction type pine wood nematode |
| CN101832889A (en) * | 2010-04-30 | 2010-09-15 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN101832889B (en) * | 2010-04-30 | 2011-11-23 | 王进福 | Sheet washing method for rapid pine wood nematode separation technology |
| CN103471901A (en) * | 2013-08-29 | 2013-12-25 | 广西大学 | Sporocyst separating and grabbing tool |
| CN103471901B (en) * | 2013-08-29 | 2016-05-25 | 广西大学 | A kind of sporangiocyst method that captures that separates |
| CN105432566A (en) * | 2015-10-30 | 2016-03-30 | 泸县农林局 | Method for rapidly detecting pine wood nematodes |
| CN105432566B (en) * | 2015-10-30 | 2018-10-26 | 泸县农林局 | A kind of method of quick detection Bursaphelenchus xylophilus |
| CN107356459A (en) * | 2017-06-20 | 2017-11-17 | 潘沧桑 | The fast separation device of pine material nematode and its detection method of disassembling cleaning |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1184479C (en) | 2005-01-12 |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: PAN CANGSANG Free format text: FORMER OWNER: XIAMEN UNIVERSITY Effective date: 20140124 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20140124 Address after: Xiamen University East Siming District of Xiamen city in Fujian province 361005 Building No. 5 Room 202 Patentee after: Pan Cangsang Address before: Xiamen City, Fujian Province, 361005 South Siming Road No. 422 Patentee before: Xiamen University |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050112 Termination date: 20150121 |
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| EXPY | Termination of patent right or utility model |