CN107356459A - The fast separation device of pine material nematode and its detection method of disassembling cleaning - Google Patents
The fast separation device of pine material nematode and its detection method of disassembling cleaning Download PDFInfo
- Publication number
- CN107356459A CN107356459A CN201710467514.1A CN201710467514A CN107356459A CN 107356459 A CN107356459 A CN 107356459A CN 201710467514 A CN201710467514 A CN 201710467514A CN 107356459 A CN107356459 A CN 107356459A
- Authority
- CN
- China
- Prior art keywords
- edge
- water
- negative pressure
- filtering
- body bottom
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/10—Devices for withdrawing samples in the liquid or fluent state
- G01N1/14—Suction devices, e.g. pumps; Ejector devices
- G01N2001/1418—Depression, aspiration
Abstract
The fast separation device of pine material nematode and its detection method of disassembling cleaning, are related to pine wood nematode.Easily disassembled and installation is provided, is easy to cleaning, it is pervasive in disk, plank and branch, make nematode by the current that negative pressure drives from the fast separation device of pine material nematode and its detection method for tieing up the disassembling cleaning for being eluted out being identified in pipe rapidly.The pervasive outer inspection in round log, plank and branch and interior inspection, hiding nematode and microorganism can be made to be eluted rapidly from dimension pipe by the current that negative pressure drives, not only making originally needed more than 24h mask work to obtain completes in minutes, it is easy to the property and species of nematode in Rapid identification timber, card speed is crossed in the clearance for greatly speeding up Wood products and wooden packing goods, also exempts the hardship that quarantine functionary's splitting is chipped；It is perfect and separator is easily disassembled and installation, is easy to clean, and so as to avoid cross-infection between sample, the Timber Quarantine in China is worked and is in a leading position in the world level, establish oneself in an unassailable position.
Description
Technical field
The present invention relates to pine wood nematode, fast separation device of pine material nematode and its detection more particularly, to disassembling cleaning
Method.
Background technology
Pine wood nematode (Bursaphelenchus xylophilus) causes pine tree wilt disease, is referred to as " smokeless forest
Fire " or " pine tree cancer ".Only just there are 1,000,000 plants of pine trees to die from pine nematode according to incomplete statistics in 2007, China,
Timber is lost more than 5,000,000 m3, add up the hm of occurring area 8.67 ten thousand2, cause 2,500,000,000 yuan of direct economic loss, indirect economic loss
About 25,000,000,000 yuan.In addition, pine nematode also threatens China large area pine forest, and some important natural history landscapes and life
State scenic spot (iron of fine quality etc., 2010, Pine Wilt Disease in China research).In the past 10 years, although putting into many manpower, material resources and financial resources,
The impetus of pine nematode sprawling diffusion is not restrained, loss continues to increase.On the one hand, because the Forest Diseases such as pine nematode
The forestry products such as pine caused by insect pest and rosin, turpentine oil are in short supply, and price sharp rises；On the other hand, with economic construction and
The development of society, ecological construction and environmental protection upgrading, most forest zones take taboo to cut down measure, and trees turn into money more and more in short supply
Source.The timber in China current more than 60% leans on import, and imported timber has the danger of incoming pest and disease damage, particularly pine wood nematode
Disease.Therefore, each port except tightly check cargoes imported and exported Wooden packing container in addition to, also to check substantial amounts of imported log and
Various woodworks.So the workload for importing and exporting inspection and quarantine is very big.
Caused disaster because Reproduction In Bursaphelenchus Xylophilus speed is fast, and by the insect-borne transmissions such as longicorn, quickly sprawling, make sheet
Pine tree is dead rapidly, therefore it is one of major measure for preventing the disease from spreading to forbid lesion timber movement.To prevent the disease from entering one
Step is spread, and inspection post is set up on main traffic thoroughfare in China various regions at present, is intercepted pine and is checked.
But nematode is isolated from timber and is identified, it is a time consuming tedious work.Usual timber takes
Small bar (also thinner than chopsticks) must be first split into after sample, is then placed in the containers such as funnel or large beaker, by modified Baermann funnel method
Principle is separated, and typically need to can just isolate nematode through 24h.In consideration of it, Chinese patent ZL02129944.7 inventor Pan
Have experienced many vicissitudes of life on January 21st, 2003 provide pine wood nematode quick disconnector, can from " disk " (trees cross section) quick separating
Go out nematode, recognize the limitation that nematode can only be separated from " disk " later, improve the separator, for " disk ", wood
Nematode in plate and branch can quick separating, and applied for Chinese patent ZL again within 1st in August in 2005
200510088062.3。
But, the pine wood nematode quick disconnector after improvement still has the shortcomings that some are very important：Due to separator
Heavy, cleaning is very bothersome, time consuming；Even if cleaning, can not also ensure thoroughly to clean, there is a possibility that cross-infection,
The nematode for not having in a upper sample in the separating liquid of second sample can not be ensured.That is use existing quick point
From device, it may occur in which that assay has the query of cross-infection possibility, therefore inventor provide the pine of disassembling cleaning
Nematode fast separation device.
The content of the invention
The purpose of the present invention aims to provide easily disassembled and installation, is easy to cleaning, pervasive in disk, plank and branch,
Make nematode quick from the pine wood nematode for tieing up the disassembling cleaning for being eluted out being identified in pipe rapidly by the current that negative pressure drives
Separator and its detection method.
First, one of fast separation device of pine material nematode of disassembling cleaning：
Provided with board, filter body, negative pressure component, depression bar and vavuum pump；
The board is made up of base, column, middle platen and cross tube, and circular hole, the aperture of the circular hole are set on middle platen
Middle and lower part external diameter with filtering body is coincide, and sealing ring is set between circular hole and suction filtration body；
The bottom for filtering body, which is provided with, filters body water valve；The negative pressure component is provided with negative pressure cavity, and the depression bar is provided with electricity
Liquid connecting rod or the screw rod with handwheel；
The suction filtration body frame is on the circular hole of middle platen, and the bottom for filtering body is stretched into negative pressure cavity, and the stage casing of negative pressure cavity is set
Have door, sealing ring be provided between the door and negative pressure cavity, can ensure when the door is closed it is air tight, when door is opened
Qi Shike is put into or taken out container, and the top of the negative pressure cavity cavity wall is provided with hole and fixed tubule and air valve, and the tubule passes through
Pressure pipe is connected with the bleeding point of vavuum pump；
The suction filtration body is made up of upper and lower part, filters body using middle platen as boundary, and the upper and lower part for filtering body is whole
Body, filters that the diameter on body top is bigger than the diameter for filtering body bottom, filter body bottom upper edge be higher by top bottom surface 0.5~
1.5cm, when suction filtration body mask work starts, the upper edge for filtering body bottom, plank or disk leaching are covered using plank or disk
Not in the water for filtering body top；
The upper edge for filtering body bottom is for even surface or in blade shape edge, if upper edge is even surface, smooth
Face is provided with sealing ring；If upper edge is blade shape edge, sealing ring need not be added；
Drying bottle is connected between the vavuum pump and negative pressure cavity, the drying bottle is built with drier.
The container can be cup or culture dish.
2nd, the two of the fast separation device of pine material nematode of disassembling cleaning：
Provided with board, filter body, negative pressure component, depression bar and vavuum pump；
The board is made up of base, column, middle platen and cross tube, and middle platen sets circular hole, the aperture of the circular hole with
The external diameter for filtering in the middle part of body and bottom is coincide, and sealing ring is set between circular hole and suction filtration body；
The negative pressure component is provided with the air-tight bottle of tool screw thread mouth, and the depression bar is provided with electro-hydraulic connecting rod or the screw rod with handwheel；
The suction filtration body frame filters body lower openings inwall and is provided with screw thread, filter body bottom on the circular hole of middle platen
Stenosis is provided with water valve, and the upper wall for filtering body bottom passes through pressure pipe and vavuum pump provided with hole and fixed tubule and air valve, tubule
Bleeding point be connected, filter body lower openings by helicoid its screw thread of bottleneck precession of air-tight bottle；
The suction filtration body is made up of upper and lower part, filters body using middle platen as boundary, and the upper and lower part for filtering body is whole
Body, filters that the diameter on body top is bigger than the diameter for filtering body bottom, filter body bottom upper edge be higher by top bottom surface 0.5~
1.5cm, when suction filtration body mask work starts, the upper edge for filtering body bottom, plank or disk leaching are covered using plank or disk
Not in the water for filtering body top；
The upper edge for filtering body bottom is for even surface or in blade shape edge, when the upper edge for filtering body bottom is even surface
When, then sealing ring is provided with even surface；When the upper edge of the suction filtration body bottom is blade shape edge, then sealing need not be added
Circle；
Sealing ring is provided between the lower openings and the spiral interface of air-tight bottle for filtering body, during ensureing vacuum pump work
Negative pressure can be realized in negative pressure construction；
Drying bottle is connected between the vavuum pump and suction filtration body bottom, the drying bottle is built with drier.
3rd, one of fast separation device of pine material nematode detection method of disassembling cleaning comprises the following steps：
1) length and width of plank is more than and filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) the one side planing of plank, plank center along with dimension manage vertical direction set together with breach, notch length is small
In the internal diameter for filtering edge on body bottom, the depth of breach is less than the thickness of plank；
In step 2), the breach can use one kind in circular saw, electric drill, emery wheel, chisel etc..
3) plank is turned upside down, makes breach down, covered and filter edge on body bottom, notch alignment edge on body bottom is filtered
Within scope；
4) start electro-hydraulic connecting rod or screw screw rod, plank is pressed in and filters edge on body bottom, plank is with filtering on body bottom
The sealing ring on edge is close to；
5) door of negative pressure cavity is opened, puts container into, opens the water valve for filtering body lower end, the door for being then shut off negative pressure cavity is close
Close；
6) clear water is poured into and filters body top, the water surface floods plank；
7) air valve of negative pressure cavity upper wall is opened, starts vavuum pump, the water for filtering body top flows into：The appearance of water receiving in negative pressure cavity
In device；
8) power supply of vavuum pump is closed, the door of negative pressure cavity is opened, closes the water valve for filtering body lower end, take out the appearance of water receiving
Device；Then the water in container is poured into culture dish, nematode is checked under anatomical lens, identify the species of nematode under the microscope；
In step 8), the microscope can use high-power microscope or inverted microscope.
9) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
10) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
4th, the two of the fast separation device of pine material nematode detection method of disassembling cleaning comprise the following steps：
1) external diameter of disk is more than and filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) disk it is smooth one down, cover the edge on body bottom is filtered, and the area of disk is on suction filtration body bottom
Edge is completely covered；
3) start electro-hydraulic connecting rod or screw screw rod, disk is pressed in and filters edge on body bottom, disk is with filtering on body bottom
The sealing ring on edge is close to；
4) door of negative pressure cavity is opened, puts container into, opens the water valve for filtering body lower end, the door for being then shut off negative pressure cavity is close
Close；
5) clear water is poured into and filters body top, the water surface floods disk；
6) air valve of negative pressure cavity upper wall is opened, starts vavuum pump, the water for filtering body top flows into：The appearance of water receiving in negative pressure cavity
In device；
7) power supply of vavuum pump is closed, the door of negative pressure cavity is opened, closes the water valve for filtering body lower end, take out the appearance of water receiving
Device；Then the water in container is poured into culture dish, nematode is checked under anatomical lens, identify the species of nematode under the microscope；
In step 7), the microscope can use high-power microscope or inverted microscope.
8) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
9) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
5th, the three of the fast separation device of pine material nematode detection method of disassembling cleaning comprise the following steps：
1) saw takes one section of plank, and its length and width, which is more than, filters the external diameter on edge on body bottom, less than suction filtration body top
Internal diameter；
2) the one side planing of plank, plank center along with timber dimension manage vertical direction set together with breach, breach length
Degree is less than the internal diameter for filtering edge on body bottom, and the depth of breach is less than the thickness of plank；
In step 2), the breach can use one kind in circular saw, electric drill, emery wheel, chisel etc..
3) plank is turned upside down, makes breach down, covered and filter edge on body bottom, notch alignment edge on body bottom is filtered
Within scope；
4) start electro-hydraulic connecting rod or screw screw rod, plank insertion is filtered on body bottom along the edge of blade shape；
5) in the screw thread mouth for threaded air-tight bottle bottleneck precession being filtered body lower end, while the water for filtering body lower end is opened
Valve；
6) clear water is poured into and filters body top, the water surface floods plank；
7) air valve for filtering body bottom upper wall is opened, starts vavuum pump, the water for filtering body top flows into air-tight bottle；
8) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture
In ware, nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
In step 8), the microscope can use high-power microscope or inverted microscope.
9) loosen electro-hydraulic connecting rod or unscrew screw rod, suction filtration body is unloaded from board, that is, carry out the separation behaviour of next plank
Make；
10) plank that separated is taken out in vivo from unloading to filter.
6th, the four of the fast separation device of pine material nematode detection method of disassembling cleaning comprise the following steps：
1) saw takes one section of disk, and its length and width, which is more than, filters the external diameter on edge on body bottom, less than suction filtration body top
Internal diameter；
2) disk it is smooth one down, cover the edge on body bottom is filtered, and the area of disk is on suction filtration body bottom
Edge is completely covered；
3) start electro-hydraulic connecting rod or screw screw rod, disk insertion is filtered on body bottom along the edge of blade shape；
4) in the screw thread mouth for threaded air-tight bottle bottleneck precession being filtered body lower end, while the water for filtering body lower end is opened
Valve；
5) clear water is poured into and filters body top, the water surface floods disk；
6) air valve for filtering body bottom upper wall is opened, starts vavuum pump, the water for filtering body top flows into air-tight bottle；
7) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture
In ware, nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
In step 7), the microscope can use high-power microscope or inverted microscope.
8) loosen electro-hydraulic connecting rod or unscrew screw rod, suction filtration body is unloaded from board, that is, carry out the separation behaviour of next disk
Make；
9) disk that separated is taken out in vivo from unloading to filter.
The pervasive outer inspection (Exit-Entry Quaratine) and interior inspection (country's inspection in round log, plank and branch of detection method of the present invention
Epidemic disease), it can be such that hiding nematode and microorganism is eluted out rapidly from dimension pipe by the current that negative pressure drives, and not only make original
Need more than 24h mask work to be completed in minutes, be easy to the property and species of nematode in Rapid identification timber, greatly
Accelerate Wood products greatly and card speed is crossed in the clearance of wooden packing goods, also exempt the hardship that quarantine functionary's splitting is chipped；And point
And installation easily disassembled from device, it is easy to clean, so as to avoid cross-infection between sample, the Timber Quarantine in China is operated in state
Be in a leading position level on border, perfect, establishes oneself in an unassailable position.
Brief description of the drawings
Fig. 1 is the structural representation of fast separation device of pine material nematode type one of the disassembling cleaning of the embodiment of the present invention.
Fig. 2 is the ring structure of the fast separation device of pine material nematode type one of the disassembling cleaning of the embodiment of the present invention
Signal.
Fig. 3 is the structural representation of fast separation device of pine material nematode type two of the disassembling cleaning of the embodiment of the present invention.
Embodiment
First, one of fast separation device of pine material nematode of disassembling cleaning (referring to Fig. 1)：
Provided with board, filter body 11, negative pressure component 21, depression bar 41 and vavuum pump；
The board is made up of base, column, middle platen 4 and cross tube, and circular hole, the hole of the circular hole are set on middle platen 4
Footpath is coincide with filtering the middle and lower part external diameter of body 11, and sealing ring 5 is set between circular hole and suction filtration body 11；
The bottom for filtering body 11, which is provided with, filters body valve；The negative pressure component is provided with negative pressure cavity 21, the depression bar 41
Screw rod provided with electro-hydraulic connecting rod or with handwheel；
The suction filtration frame of body 11 is on the circular hole of middle platen 4, and the bottom for filtering body 11 is stretched into negative pressure cavity 21, negative pressure cavity 21
Stage casing be provided with door, sealing ring 5 is provided between the door and negative pressure cavity 21, can be ensured not when the door is closed
Gas leakage, can be put into or take out when the door is open container 31, and the top of the cavity wall of negative pressure cavity 21 is provided with hole and fixed tubule and gas
Valve 6, the tubule are connected by pressure pipe with the bleeding point of vavuum pump；
The suction filtration body 11 is made up of upper and lower part, and it is boundary to filter body 11 with middle platen 4, filters the top of body 11 with
To be overall, the diameter on suction filtration body 11 top is bigger than the diameter for filtering the bottom of body 11 in portion, and the upper edge for filtering the bottom of body 11 is higher by top
0.5~1.5cm of bottom surface, when the suction filtration mask work of body 11 starts, the upper edge for filtering the bottom of body 11 is covered using plank or disk,
Plank or disk are immersed in the water for filtering the top of body 11；
The upper edge for filtering the bottom of body 11 is for even surface or in blade shape edge, if upper edge is even surface, flat
Sliding surface is provided with sealing ring 5；If upper edge is blade shape edge, sealing ring need not be added；
Drying bottle is connected between the vavuum pump and negative pressure cavity 21, the drying bottle is built with drier；
The container 31 is cup or culture dish.
2nd, two (referring to Fig. 3) of the fast separation device of pine material nematode of disassembling cleaning：
Provided with board, filter body 11, negative pressure component 22, depression bar 41 and vavuum pump；
The board is made up of base, column, middle platen 4 and cross tube, and middle platen 4 sets circular hole, the aperture of the circular hole
External diameter with filtering the middle part of body 11 and bottom is coincide, and sealing ring 5 is set between circular hole and suction filtration body 11；
The negative pressure component 22 is provided with the air-tight bottle of tool screw thread mouth, and the depression bar 41 is provided with electro-hydraulic connecting rod or the spiral shell with handwheel
Bar；
The suction filtration frame of body 11 filters the lower openings inwall of body 11 and is provided with screw thread 32, filter body on the circular hole of middle platen 4
The stenosis of 11 bottoms is provided with water valve, and the upper wall for filtering the bottom of body 11 is provided with hole and fixed tubule and valve 6, and tubule passes through pressure-resistant
Pipe is connected with the bleeding point of vavuum pump, filters body lower openings by helicoid its screw thread of bottleneck precession of air-tight bottle；
The suction filtration body 11 is made up of upper and lower part, and it is boundary to filter body 11 with middle platen 4, filters the top of body 11 with
To be overall, the diameter on suction filtration body 11 top is bigger than the diameter for filtering the bottom of body 11 in portion, and the upper edge for filtering the bottom of body 11 is higher by top
0.5~1.5cm of bottom surface, when the suction filtration mask work of body 11 starts, the upper edge for filtering the bottom of body 11 is covered using plank or disk,
Plank or disk are immersed in the water for filtering the top of body 11；
The upper edge for filtering the bottom of body 11 is for even surface or in blade shape edge, when the upper edge for filtering the bottom of body 11 is flat
During sliding surface, then sealing ring 5 is provided with even surface；When the upper edge of suction filtration body 11 bottom is blade shape edge, then need not
Add sealing ring；
Sealing ring is provided between the lower openings and the spiral interface of air-tight bottle for filtering body 11, to ensure vacuum pump work
When negative pressure construction in can realize negative pressure；
Drying bottle is connected between the vavuum pump and suction filtration body 11 bottom, the drying bottle is built with drier.
3rd, one of fast separation device of pine material nematode detection method of disassembling cleaning comprises the following steps：
1) length and width of plank is more than and filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) the one side planing of plank, plank center along with dimension manage vertical direction chisel out together with breach, breach length
Degree is less than the internal diameter for filtering edge on body bottom, and the depth of breach is less than the thickness of plank；
In step 2), the breach can use one kind in circular saw, electric drill, emery wheel, chisel etc..
3) plank chiseled is turned upside down, makes breach down, covered and filter edge on body bottom, notch alignment is in the case where filtering body
In portion within the scope of；
4) start electro-hydraulic connecting rod or screw screw rod, plank is pressed in and filters edge on body bottom, plank is with filtering on body bottom
The sealing ring on edge is close to；
5) door of negative pressure cavity is opened, puts container into, opens the water valve for filtering body lower end, the door for being then shut off negative pressure cavity is close
Close；
6) clear water is poured into and filters body top, the water surface floods plank；
7) air valve of negative pressure cavity upper wall is opened, starts vavuum pump, the water for filtering body top flows into：The appearance of water receiving in negative pressure cavity
In device；
8) power supply of vavuum pump is closed, the door of negative pressure cavity is opened, closes the water valve for filtering body lower end, take out the appearance of water receiving
Device；Then the water in container is poured into culture dish, nematode is checked under anatomical lens, identify the species of nematode under the microscope；
In step 8), the microscope can use high-power microscope or inverted microscope.
9) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
10) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
4th, the two of the fast separation device of pine material nematode detection method of disassembling cleaning comprise the following steps：
1) external diameter of disk is more than and filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) disk it is smooth one down, cover the edge on body bottom is filtered, and the area of disk is on suction filtration body bottom
Edge is completely covered；
3) start electro-hydraulic connecting rod or screw screw rod, disk is pressed in and filters edge on body bottom, disk is with filtering on body bottom
The sealing ring on edge is close to；
4) door of negative pressure cavity is opened, puts container into, opens the water valve for filtering body lower end, the door for being then shut off negative pressure cavity is close
Close；
5) clear water is poured into and filters body top, the water surface floods disk；
6) air valve of negative pressure cavity upper wall is opened, starts vavuum pump, the water for filtering body top flows into：The appearance of water receiving in negative pressure cavity
In device；
7) power supply of vavuum pump is closed, the door of negative pressure cavity is opened, closes the water valve for filtering body lower end, take out the appearance of water receiving
Device；Then the water in container is poured into culture dish, nematode is checked under anatomical lens, identify the species of nematode under the microscope；
In step 7), the microscope can use high-power microscope or inverted microscope.
8) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
9) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
5th, the three of the fast separation device of pine material nematode detection method of disassembling cleaning comprise the following steps：
1) saw takes one section of plank, and its length and width, which is more than, filters the external diameter on edge on body bottom, less than suction filtration body top
Internal diameter；
2) the one side planing of plank, plank center along with timber dimension manage vertical direction chisel out together with breach, lack
Mouth length is less than the internal diameter for filtering edge on body bottom, and the depth of breach is less than the thickness of plank；
In step 2), the breach can use one kind in circular saw, electric drill, emery wheel, chisel etc..
3) plank chiseled is turned upside down, makes breach down, covered and filter edge on body bottom, notch alignment is in the case where filtering body
In portion within the scope of；
4) start electro-hydraulic connecting rod or screw screw rod, plank insertion is filtered on body bottom along the edge of blade shape；
5) in the screw thread mouth for threaded air-tight bottle bottleneck precession being filtered body lower end, while the water for filtering body lower end is opened
Valve；
6) clear water is poured into and filters body top, the water surface floods plank；
7) air valve for filtering body bottom upper wall is opened, starts vavuum pump, the water for filtering body top flows into air-tight bottle；
8) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture
In ware, nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
In step 8), the microscope can use high-power microscope or inverted microscope.
8) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture
In ware, nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
In step 8), the microscope can use high-power microscope or inverted microscope.
9) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
10) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
6th, the four of the fast separation device of pine material nematode detection method of disassembling cleaning comprise the following steps：
1) saw takes one section of disk, and its length and width, which is more than, filters the external diameter on edge on body bottom, less than suction filtration body top
Internal diameter；
2) disk it is smooth one down, cover the edge on body bottom is filtered, and the area of disk is on suction filtration body bottom
Edge is completely covered；
3) start electro-hydraulic connecting rod or screw screw rod, disk insertion is filtered on body bottom along the edge of blade shape；
4) in the screw thread mouth for threaded air-tight bottle bottleneck precession being filtered body lower end, while the water for filtering body lower end is opened
Valve；
5) clear water is poured into and filters body top, the water surface floods disk；
6) air valve for filtering body bottom upper wall is opened, starts vavuum pump, the water for filtering body top flows into air-tight bottle；
7) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture
In ware, nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
In step 7), the microscope can use high-power microscope or inverted microscope.
8) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture
In ware, nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
In step 8), the microscope can use high-power microscope or inverted microscope.
9) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
10) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
Specific embodiment given below.
Embodiment 1
Type one：Its basic structure includes：Board, filter body 11, negative pressure cavity 21, depression bar 41 and vavuum pump.
Negative pressure cavity is arranged on below middle platen 4, and the stage casing of negative pressure cavity 21 sets a switchable door, between door and negative pressure cavity
If sealing ring , can ensure air tight when the door is closed, container 31 (one can be put into or taken out when the door is open
Secondary property cup or culture dish).
Filter the fixed valve in mouth top under body.The top of negative pressure cavity cavity wall will be drilled with hole and fixed tubule, tubule pass through
Pressure pipe is connected with the bleeding point of vavuum pump.The upper and lower part for filtering body is an entirety, and can be placed in middle
On plate 4, suction filtration body bottom is set to stretch into negative pressure cavity 21.
The diameter on suction filtration body top is bigger than the diameter of bottom, and the upper edge of bottom is higher by the ㎝ of bottom surface 0.5~1.5 on top, makes
The upper edge of bottom can be immersed in the water for filtering body top, while the plank to be separated or disk can be made to be immersed in and filter on body
In the water in portion.
The upper edge for filtering body bottom is for even surface or in blade shape edge；Sealing is then set on even surface if the former
Circle, sealing ring need not be added if the latter.It is both set to be sealed between wood sample and air tight.Meanwhile
Filter and put a sealing ring between the big hole of body 11 and middle platen 4, can be real in negative pressure construction during ensureing vacuum pump work
Existing negative pressure.
In addition, being to prevent steam from entering vavuum pump, one is connected between vavuum pump and negative pressure cavity 21 drier is housed
Drying bottle.
Embodiment 2
Type two：Including：Board, air-tight bottle 22, depression bar 41 and the vavuum pump for filtering body 11, having screw thread mouth.
Negative pressure cavity is not set, instead has the air-tight bottle 22 of screw thread mouth.Suitably shorten and filter body, while simultaneously car goes out the lower mouth of expansion
Screw thread, it is allowed to coincide with the screw thread mouth of air-tight bottle.
Filter " ankle " (stenosis) under body near mouth and want a fixed valve.The upper wall for filtering body bottom is drilled with Kong Binggu
If tubule, tubule is connected by pressure pipe with the bleeding point of vavuum pump.The upper and lower part for filtering body is an entirety, and
It can be placed on middle platen 4, allow in the screw thread of its lower mouth of the helicoid bottleneck precession of air-tight bottle 22.
The diameter on suction filtration body top is bigger than the diameter of bottom, and the upper edge of bottom is higher by the ㎝ of bottom surface 0.5~1.5 on top, makes
The upper edge of bottom can be immersed in the water for filtering body top, while the plank to be separated or disk is immersed in suction filtration body
In the water on top.
The upper edge for filtering body bottom is for even surface or in blade shape edge, when the upper edge for filtering body bottom is even surface
When, then sealing ring is provided with even surface；When the upper edge of the suction filtration body bottom is blade shape edge, then sealing need not be added
Circle.And be provided with sealing ring between the bottleneck of lower mouth and air-tight bottle 22 of body is filtered, negative pressure construction during ensureing vacuum pump work
It is interior to realize negative pressure.In addition, to prevent steam from entering vavuum pump, connection one can be filled between vavuum pump and suction filtration body bottom
There is the drying bottle of drier.
Claims (10)
1. the fast separation device of pine material nematode of disassembling cleaning, it is characterised in that provided with board, filter body, negative pressure component, pressure
Bar and vavuum pump；
The board is made up of base, column, middle platen and cross tube, circular hole is set on middle platen, the aperture of the circular hole is with taking out
The middle and lower part external diameter of filter body is coincide, and sealing ring is set between circular hole and suction filtration body；
The bottom for filtering body, which is provided with, filters body valve；The negative pressure component is provided with negative pressure cavity, and the depression bar is provided with electro-hydraulic company
Bar or the screw rod with handwheel；
The suction filtration body frame is on the circular hole of middle platen, and the bottom for filtering body is stretched into negative pressure cavity, and the stage casing of negative pressure cavity is provided with door,
Sealing ring is provided between the door and negative pressure cavity, can ensure when the door is closed it is air tight, when the door is open
Container can be put into or take out, the top of the negative pressure cavity cavity wall is provided with hole and fixed tubule and valve, and the tubule passes through pressure-resistant
Pipe is connected with the bleeding point of vavuum pump；
The suction filtration body is made up of upper and lower part, filters body using middle platen as boundary, and it is overall to filter the upper and lower part of body, is taken out
The diameter on filter body top is bigger than the diameter for filtering body bottom, and the upper edge for filtering body bottom is higher by 0.5~1.5cm of top bottom surface, when
When suction filtration body mask work starts, the upper edge for filtering body bottom is covered using plank or disk, plank or disk are immersed in suction filtration
In the water on body top；
The upper edge for filtering body bottom is for even surface or in blade shape edge, if upper edge is even surface, on even surface
Provided with sealing ring；If upper edge is blade shape edge, sealing ring need not be added；
Drying bottle is connected between the vavuum pump and negative pressure cavity, the drying bottle is built with drier；
The container is cup or culture dish.
2. the fast separation device of pine material nematode of disassembling cleaning, it is characterised in that provided with board, filter body, negative pressure component, pressure
Bar and vavuum pump；
The board is made up of base, column, middle platen and cross tube, and middle platen sets circular hole, the aperture of the circular hole and suction filtration
The external diameter of in the middle part of body and bottom is coincide, and sealing ring is set between circular hole and suction filtration body；
The negative pressure component is provided with the air-tight bottle of tool screw thread mouth, and the depression bar is provided with electro-hydraulic connecting rod or the screw rod with handwheel；
The suction filtration body frame filters body lower openings inwall and is provided with screw thread, filter the narrow of body bottom on the circular hole of middle platen
Place is provided with water valve, and the upper wall of suction filtration body bottom is provided with hole and fixed tubule and air valve, and tubule is taken out by pressure pipe and vavuum pump
Gas port is connected, and filters the air-tight bottle that body lower openings are used for precession tool screw thread bottleneck；
The suction filtration body is made up of upper and lower part, filters body using middle platen as boundary, and it is overall to filter the upper and lower part of body, is taken out
The diameter on filter body top is bigger than the diameter for filtering body bottom, and the upper edge for filtering body bottom is higher by 0.5~1.5cm of top bottom surface, when
When suction filtration body mask work starts, the upper edge for filtering body bottom is covered using plank or disk, plank or disk are immersed in suction filtration
In the water on body top；
The upper edge for filtering body bottom is for even surface or in blade shape edge, when the upper edge for filtering body bottom is even surface,
Sealing ring is then provided with even surface；When the upper edge of the suction filtration body bottom is blade shape edge, then sealing ring need not be added；
Sealing ring is provided between the lower openings and the spiral interface of air-tight bottle for filtering body, negative pressure during ensureing vacuum pump work
Negative pressure can be realized in construction；
Drying bottle is connected between the vavuum pump and suction filtration body bottom, the drying bottle is built with drier.
3. the fast separation device of pine material nematode detection method of disassembling cleaning, it is characterised in that comprise the following steps：
1) length and width of plank is more than and filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) the one side planing of plank, breach is set along the direction vertical with dimension pipe in plank center, notch length, which is less than, filters body
The internal diameter on edge on bottom, the depth of breach are less than the thickness of plank；
3) plank is turned upside down, makes breach down, covered and filter edge on body bottom, the scope on notch alignment edge on body bottom is filtered
Within；
4) start electro-hydraulic connecting rod or screw screw rod, plank is pressed in and filters edge on body bottom, plank is with filtering edge on body bottom
Sealing ring is close to；
5) door of negative pressure cavity is opened, puts container into, opens the water valve for filtering body lower end, the door for being then shut off negative pressure cavity is closed；
6) clear water is poured into and filters body top, the water surface floods plank；
7) air valve of negative pressure cavity upper wall is opened, starts vavuum pump, the water for filtering body top flows into：The container of water receiving in negative pressure cavity
It is interior；
8) power supply of vavuum pump is closed, the door of negative pressure cavity is opened, closes the water valve for filtering body lower end, take out the container of water receiving；So
The water in container is poured into culture dish afterwards, nematode is checked under anatomical lens, identifies the species of nematode under the microscope；
9) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
10) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
4. the fast separation device of pine material nematode detection method of disassembling cleaning as claimed in claim 3, it is characterised in that in step
It is rapid 2) in, the breach is using circular saw, electric drill, emery wheel, one kind in chisel；In step 8), the microscope is using high
Power microscope or inverted microscope.
5. the fast separation device of pine material nematode detection method of disassembling cleaning, it is characterised in that comprise the following steps：
1) external diameter of disk is more than and filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) disk it is smooth one down, cover the edge on body bottom is filtered, and the area of disk on suction filtration body bottom along complete
All standing；
3) start electro-hydraulic connecting rod or screw screw rod, disk is pressed in and filters edge on body bottom, disk is with filtering edge on body bottom
Sealing ring is close to；
4) door of negative pressure cavity is opened, puts container into, opens the water valve for filtering body lower end, the door for being then shut off negative pressure cavity is closed；
5) clear water is poured into and filters body top, the water surface floods disk；
6) air valve of negative pressure cavity upper wall is opened, starts vavuum pump, the water for filtering body top flows into：The container of water receiving in negative pressure cavity
It is interior；
7) power supply of vavuum pump is closed, the door of negative pressure cavity is opened, closes the water valve for filtering body lower end, take out the container of water receiving；So
The water in container is poured into culture dish afterwards, nematode is checked under anatomical lens, identifies the species of nematode under the microscope；
8) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
9) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
6. the fast separation device of pine material nematode detection method of disassembling cleaning as claimed in claim 5, it is characterised in that in step
It is rapid 7) in, the microscope uses high-power microscope or inverted microscope.
7. the fast separation device of pine material nematode detection method of disassembling cleaning, it is characterised in that comprise the following steps：
1) saw takes one section of plank, and its length and width, which is more than, filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) the one side planing of plank, breach is set along the direction vertical with timber dimension pipe in plank center, notch length, which is less than, takes out
The internal diameter on edge on filter body bottom, the depth of breach are less than the thickness of plank；
3) plank is turned upside down, makes breach down, covered and filter edge on body bottom, the scope on notch alignment edge on body bottom is filtered
Within；
4) start electro-hydraulic connecting rod or screw screw rod, plank insertion is filtered on body bottom along the edge of blade shape；
5) in the screw thread mouth for threaded air-tight bottle bottleneck precession being filtered body lower end, while the water valve for filtering body lower end is opened；
6) clear water is poured into and filters body top, the water surface floods plank；
7) air valve for filtering body bottom upper wall is opened, starts vavuum pump, the water for filtering body top flows into air-tight bottle；
8) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture dish,
Nematode is checked under anatomical lens, and then identifies the species of nematode under the microscope；
9) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
10) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
8. the fast separation device of pine material nematode detection method of disassembling cleaning as claimed in claim 7, it is characterised in that in step
It is rapid 2) in, the breach is using circular saw, electric drill, emery wheel, one kind in chisel；In step 8), the microscope is using high
Power microscope or inverted microscope.
9. the fast separation device of pine material nematode detection method of disassembling cleaning, it is characterised in that comprise the following steps：
1) saw takes one section of disk, and its length and width, which is more than, filters the external diameter on edge on body bottom, less than the internal diameter for filtering body top；
2) disk it is smooth one down, cover the edge on body bottom is filtered, and the area of disk on suction filtration body bottom along complete
All standing；
3) start electro-hydraulic connecting rod or screw screw rod, disk insertion is filtered on body bottom along the edge of blade shape；
4) in the screw thread mouth for threaded air-tight bottle bottleneck precession being filtered body lower end, while the water valve for filtering body lower end is opened；
5) clear water is poured into and filters body top, the water surface floods disk；
6) air valve for filtering body bottom upper wall is opened, starts vavuum pump, the water for filtering body top flows into air-tight bottle；
7) power supply of vavuum pump is closed, closes the water valve for filtering body bottom, air-tight bottle is screwed out, then, water is poured into culture dish,
The species of nematode is identified under the microscope；
8) bleed off and filter body upper water, loosen electro-hydraulic connecting rod or unscrew screw rod, take out the sample separated and abandon it；
9) suction filtration body is unloaded from board, the lock out operation of next sample is carried out after cleaning.
10. the fast separation device of pine material nematode detection method of disassembling cleaning as claimed in claim 9, it is characterised in that in step
It is rapid 7) in, the microscope uses high-power microscope or inverted microscope.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710467514.1A CN107356459B (en) | 2017-06-20 | 2017-06-20 | Detachable and washable fast pine wood nematode separating device and detection method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710467514.1A CN107356459B (en) | 2017-06-20 | 2017-06-20 | Detachable and washable fast pine wood nematode separating device and detection method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107356459A true CN107356459A (en) | 2017-11-17 |
| CN107356459B CN107356459B (en) | 2020-09-15 |
Family
ID=60272406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710467514.1A Expired - Fee Related CN107356459B (en) | 2017-06-20 | 2017-06-20 | Detachable and washable fast pine wood nematode separating device and detection method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107356459B (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1430061A (en) * | 2003-01-21 | 2003-07-16 | 厦门大学 | Fast separation device of pine material nematode and its detection method |
| CN1731130A (en) * | 2005-08-01 | 2006-02-08 | 厦门大学 | Apparatus for quick separation of pine wood nematode and detection method therefor |
| CN101813582A (en) * | 2010-04-01 | 2010-08-25 | 北京市农林科学院 | Integrated pesticide residue detection pretreatment device and pretreatment method |
| CN202614577U (en) * | 2011-10-14 | 2012-12-19 | 南京生兴生物技术有限公司 | Differential pressure method pine wood nematode quick separation device |
-
2017
- 2017-06-20 CN CN201710467514.1A patent/CN107356459B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1430061A (en) * | 2003-01-21 | 2003-07-16 | 厦门大学 | Fast separation device of pine material nematode and its detection method |
| CN1731130A (en) * | 2005-08-01 | 2006-02-08 | 厦门大学 | Apparatus for quick separation of pine wood nematode and detection method therefor |
| CN101813582A (en) * | 2010-04-01 | 2010-08-25 | 北京市农林科学院 | Integrated pesticide residue detection pretreatment device and pretreatment method |
| CN202614577U (en) * | 2011-10-14 | 2012-12-19 | 南京生兴生物技术有限公司 | Differential pressure method pine wood nematode quick separation device |
Non-Patent Citations (1)
| Title |
|---|
| 黄亚东: "《微生物实验技术》", 31 January 2013, 中国轻工业出版社 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107356459B (en) | 2020-09-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Wutscher et al. | 572 Vol. 61, No. 7--PLANT DISEASE REPORTER--July 1977 ZINC AND WATER-SOLUBLE PHENOLIC LEVELS IN THE WOOD FOR THE DIAGNOSIS OF CITRUS BLIGHT | |
| Arhipova et al. | Butt rot incidence, causal fungi, and related yield loss in Picea abies stands of Latvia | |
| Zhurbenko et al. | A first synopsis of lichenicolous fungi of Mongolia, with the description of five new species | |
| ES2572602T1 (en) | Fermentation procedure and apparatus | |
| Zhurbenko | Lichenicolous fungi from Far East of Russia | |
| CN107356459A (en) | The fast separation device of pine material nematode and its detection method of disassembling cleaning | |
| CN1184479C (en) | Fast separation device of pine material nematode and its detection method | |
| CN103773598A (en) | Quick supercritical oil extraction method | |
| Magnago et al. | A new species of Gyroporus (Gyroporaceae, Boletales) from Atlantic forest in southern Brazil | |
| CN100397062C (en) | Apparatus for quick separation of pine wood nematode and detection method therefor | |
| DE527679T1 (en) | GRAPE JUICER AND METHOD FOR JUICEING GRAPE USE OF THIS JUICIFIER. | |
| Yang et al. | Internal cause analysis of damage of wooden components in danxia temple ancient architectures: Tree species | |
| Lumbsch et al. | A revision of Pertusaria species with hyaline ascospores in southwestern North America (Pertusariales, Ascomycotina) | |
| CN205249993U (en) | Juice device is got to rubber tree high efficiency | |
| CN2811976Y (en) | Portable apparatus for quickly separating pine wood nematode | |
| CN208341361U (en) | A kind of saline and alkaline prone soil repairing and treating observation device | |
| CN207366807U (en) | A kind of dual-purpose optical fiber splitter | |
| CN108254515A (en) | A kind of portable unit of water environment pH value detection | |
| Dhingra | Diversity of resupinate, non-poroid agaricomycetous fungi in the Himalaya and adjoining areas. | |
| Buchanan et al. | New Zealand polypore fungi: six new species and a redetermination | |
| Jančovičová et al. | Panellus ringens and P. violaceofulvus (Agaricales, Mycenaceae) from Slovakia: morphological and ecological aspects. | |
| CN110127095A (en) | A kind of filtering liquor filling line generates the alcoholic device and its process of miscellaneous wine | |
| Kedrov et al. | Scalariform tracheids in secondary xylem of woody dicotyledons: distribution, function and evolutionary significance | |
| CN213549165U (en) | Longicorn insect-raising cage | |
| CN101832889B (en) | Sheet washing method for rapid pine wood nematode separation technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200915 |