CN202614577U - Differential pressure method pine wood nematode quick separation device - Google Patents
Differential pressure method pine wood nematode quick separation device Download PDFInfo
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- CN202614577U CN202614577U CN 201120390980 CN201120390980U CN202614577U CN 202614577 U CN202614577 U CN 202614577U CN 201120390980 CN201120390980 CN 201120390980 CN 201120390980 U CN201120390980 U CN 201120390980U CN 202614577 U CN202614577 U CN 202614577U
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- solenoid valve
- vacuum
- pine
- separation device
- nematode
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Abstract
The utility model discloses a differential pressure method pine wood nematode quick separation device which is applied to quick separation of nematodes in pine samples in different shapes. The differential pressure method pine wood nematode quick separation device mainly comprises a water storage tank, a support, a connector, a separation tube, a liquid collecting bottle, a vacuum line solenoid valve, a time relay, a surge flask, a vacuum switch, a waste liquid discharge solenoid valve, a vacuum pump and the like. The connector and the separation tube are directly communicated with the liquid collecting bottle and are in hollow sealing connection with each other. The inner walls of corresponding pipelines are smooth and no dead cavity exists on the inner walls of the corresponding pipelines. The vacuum switch can be used to control differential pressures. The time relay can be used to control separation time. When the differential pressure method pine wood nematode quick separation device is used, separated nematodes can be prevented from being over deformed; cross contamination between different batches of samples is effectively eliminated; and meanwhile reliability, accuracy and repeatability of nematode separation results can be improved.
Description
Technical field
The utility model relates to a kind of tripping device, is used for the quick separation of wood sample pine wood nematode, belongs to pine wood nematode pick-up unit field.
Background technology
Pine nematode is claimed pine tree wilt disease again, is a kind of crushing epidemic disease of pine tree.This harmful destructiveness of being critically ill is: pathogenicity is strong, and the dead speed of host is fast; Propagate soon, and usually be taken by surprise; In case take place, difficulty of governance is big.Therefore, it is vital the pine wood nematode of wood sample inside being quarantined fast.
Present domestic relevant quarantine departments is used the graceful funnel method of Bel more at large and is carried out the pine wood nematode separation, and this method generally needs 24 hours.The patent of inventions such as Pan Cangsang (fast separation device and the detection methods thereof of 200510088062.3 1 kinds of pine wood nematodes of the patent No.: ZL) foreshortens to several minutes with the disengaging time of nematode in the timber, thereby has improved work efficiency greatly.But this device tube chamber is succinct inadequately, has some dead spaces to be difficult for cleaning, and the possibility that causes cross pollution between sample is arranged; Pressure in the vacuum line can not be controlled, and can not overcome the distortion that intend to separate nematode well and the problem judged by accident when causing microscopy; And the running time can't be provided with, thereby might have influence on the repeatability of detection.
The utility model content
To the deficiency of prior art, the utility model---the pressure differential method fast separation device of pine material nematode is to be solved to be: the cross pollution between sample, intend to separate the excessive deformation of nematode and the later stage microscopy False Rate that possibly cause and nematode separating resulting batch in problem such as sum of errors repeatability.
For realizing above technical purpose, the utility model will be taked following technical scheme:
Adopt negative pressure mode to make the nematode in the pine sample microtubule take out of with the current that pressure reduction forms.This device is made up of aqua storage tank, support, joint, separator tube, liquid-collecting bottle, vacuum line solenoid valve, the time relay, three-in-one switch, waste liquid eliminating solenoid valve, surge flask, vacuum switch, vacuum pump, master switch.By the negative pressure that vacuum pump produces, get rid of solenoid valve, liquid-collecting bottle, separator tube, joint through vacuum switch, surge flask, waste liquid, the nematode in the water-immersed pine sample microtubule is extracted out
Vacuum switch is connected with vacuum pump, and the setting that limits numerical value through vacuum switch is controlled the pressure of vacuum pump output.To guarantee the reasonable pressure of system, reduce the distortion of nematode.
Joint is that tapering is connected with separator tube, and straight-through liquid-collecting bottle, and its annexation is that hollow sealing connects, and the corresponding pipeline inwall is level and smooth, no dead space, is convenient to clean the cross-contamination issue that can overcome between the different batches sample.
The time relay and waste liquid are got rid of solenoid valve and are connected in series; Through time set to the time relay, control
To the suction time of pine sample, to guarantee suitable imbibition quantity.
Get rid of solenoid valve and three-in-one switch of vacuum line solenoid valve parallel connection at waste liquid, with the switching of control vacuum air-channel, drain line.
According to above technical scheme, can realize following beneficial effect:
Succinct pipeline is convenient to clean the cross-contamination issue that can overcome between the different batches sample; The control vacuum pressure can avoid separating the excessive deformation of back nematode; The application of time relay circuit can quantize working routine, helps solving the problems such as batch interior sum of errors repeatability of nematode separating resulting.
Description of drawings
Fig. 1 is the structural representation of first embodiment.
Fig. 2 is first and the electronic circuit synoptic diagram of second embodiment.
Fig. 3 is the structural representation of second embodiment.
The map title and figure number: pine sample 1, water 2, joint 3, separator tube 4, aqua storage tank 5, wall of computer case 6, support 7, vacuum pump 8, vacuum switch 9, waste liquid are got rid of solenoid valve 10, vacuum line solenoid valve 11, liquid-collecting bottle 12, surge flask 13, the time relay 14, three-in-one switch 15, master switch 16.
Embodiment
Accompanying drawing discloses the structural representation of the related preferred embodiment of the utility model without limitation, below will combine accompanying drawing that the technical scheme of the utility model at length is described.
Embodiment 1:
Like Fig. 1, shown in Figure 2; The utility model---pressure differential method fast separation device of pine material nematode; Negative pressure by vacuum pump 8 generations; Get rid of solenoid valve 10, liquid-collecting bottle 12, separator tube 4, joint 3 through vacuum switch 9, surge flask 13, waste liquid, the nematode in pine sample 1 microtubule that immerses in the water 2 is extracted out.
Joint 3 and separator tube 4 are for tapering is connected, and straight-through liquid-collecting bottle 12, and its annexation is that hollow sealing connects, and the corresponding pipeline inwall is level and smooth, no dead space, is convenient to clean the cross-contamination issue that can overcome between the different batches sample.
The time relay 14 is got rid of solenoid valve 10 with waste liquid and is connected in series; Through the time set to the time relay 14, control is to the suction time of pine sample 1, to guarantee suitable imbibition quantity.
Get rid of three-in-one switch 15 of solenoid valve 10 and 11 parallel connections of vacuum line solenoid valve at waste liquid, with the switching of control vacuum air-channel, drain line.
The concrete method of application of this embodiment is following:
1) get pine sample 1, sampling requirement: thickness is 1.5-2.5cm, and the scope of length and width is 3-10cm, flawless.
2) middle position at pine sample 1 carries out pre-service with the special jewel milling cutter earlier.
Attention: be sure not during boring pine sample 1 is drilled!
3) pine sample 1 is loaded on support 7 and the joint 3, and is just adorning, fixing pine sample 1 tight through the strap bolt handle of support 7 tops.
4) will fix the support 7 of pine sample 1, and insert through the awl mouth joint on its joint 3 in the taper hole on separator tube 4 tops, connection puts in place.
5) the three-in-one switch 15 of inspection is in " neutral gear " position, in aqua storage tank 5, injects clear water, until covering pine sample 1 upper edge.
6) liquid-collecting bottle 12 that below separator tube 4, spins, dress just, screw.
7) regulate 14 working times of the time relay, scope is: 2-6 minute.
8) open master switch 15, this moment, vacuum pump 8 was started working.
9) regulate vacuum switch 9 switches, set vacuum pressure scope (can adjust) according to the nematode separating effect.
When 10) treating that vacuum switch 9 display pointers arrive in the preset pressure scope, switch is gone to " separation " gear by " neutral gear ", this moment, nematode separated beginning.Water 2 in the aqua storage tank 5 can see through pine sample 1, through joint 3, the separator tube 4 on the support 7, flows in the liquid-collecting bottle 12, and these current can be brought the nematode in pine sample 1 microtubule in the liquid-collecting bottle 12 into.
11) behind relay 14 setting-up times time of arrival, this separation device is out of service automatically.Three-in-one switch 15 is gone to " neutral gear " position.Screw off liquid-collecting bottle 12, contain the pine wood nematode of separating in the water in the liquid-collecting bottle 12.
12) three-in-one switch 15 is gone to " discharge opeing " gear, after waiting to get rid of all waste liquids in the aqua storage tank, remove the support that pine sample 1 is housed; Three-in-one switch 15 is gone to " neutral gear " position, with flushing with clean water aqua storage tank 5 and separator tube 4.
13) close master switch 16, shutdown is taken off pine sample 1, joint 3 from support 7, and cleans.Accomplish the mask work of this nematode.
Embodiment 2:
This embodiment can be applicable to branch or irregularly shaped wooden unit class pine sample, and with embodiment 1 difference be: joint 3 tops have spiral interface.
The concrete method of application of this embodiment is following:
1) get pine sample 1, sampling requirement: the diameter of branch and length range are 3-10cm, and more then it is no more than 6cm highly at most like the diameter of branch; The length and the width range of wooden unit class sample are 3-10cm, are no more than 6cm highly at most; Flawless.
2) in the central authorities of pine sample 1, comparatively flat position (side of branch or end face) is earlier through the pre-service of special jewel milling cutter.
Attention: be sure not during boring pine sample 1 is drilled!
3) through special spanner joint 3 (the plain cushion O-ring seal is housed) spinning is advanced in the brill hole milling of pine sample 1 then, required: just adorning, tightening up, to guarantee sealing.
4) fixed the joint 3 of pine sample 1, inserted in the taper hole on separator tube 4 tops through its bottom awl mouth joint, connection puts in place.
All the other operations are undertaken by the 5th～13 operation steps among the embodiment 1.
Claims (4)
1. pressure differential method fast separation device of pine material nematode; Adopt negative pressure mode to make the nematode in the pine sample microtubule take out of, form by aqua storage tank (5), support (7), joint (3), separator tube (4), liquid-collecting bottle (12), vacuum line solenoid valve (11), the time relay (14), three-in-one switch (15), waste liquid eliminating solenoid valve (10), surge flask (13), vacuum switch (9), vacuum pump (8), master switch (16) with the current that pressure reduction forms.It is characterized in that: by the negative pressure of vacuum pump (8) generation; Get rid of solenoid valve (10), liquid-collecting bottle (12), separator tube (4), joint (3) through vacuum switch (9), surge flask (13), waste liquid, the nematode that will immerse in pine sample (1) microtubule in the water (2) is extracted out.
2. pressure differential method fast separation device of pine material nematode according to claim 1, it is characterized in that being: vacuum switch (9) is connected with vacuum pump (8), and the setting that limits numerical value through vacuum switch (9) is controlled the pressure of vacuum pump (8) output.
3. pressure differential method fast separation device of pine material nematode according to claim 1; It is characterized in that being: said joint (3) is connected for tapering with separator tube (4); And straight-through liquid-collecting bottle (12), its annexation is that hollow sealing connects, and the corresponding pipeline inwall is level and smooth, no dead space.
4. pressure differential method fast separation device of pine material nematode according to claim 1, it is characterized in that: the time relay (14) is got rid of solenoid valve (10) with waste liquid and is connected in series; Through the time set to the time relay (14), control is to the suction time of pine sample (1), and vacuum line solenoid valve (11) is got rid of solenoid valve (10) with waste liquid and is connected in parallel, and the two switching is controlled by three-in-one switch (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120390980 CN202614577U (en) | 2011-10-14 | 2011-10-14 | Differential pressure method pine wood nematode quick separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120390980 CN202614577U (en) | 2011-10-14 | 2011-10-14 | Differential pressure method pine wood nematode quick separation device |
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| Publication Number | Publication Date |
|---|---|
| CN202614577U true CN202614577U (en) | 2012-12-19 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120390980 Expired - Lifetime CN202614577U (en) | 2011-10-14 | 2011-10-14 | Differential pressure method pine wood nematode quick separation device |
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| Country | Link |
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| CN (1) | CN202614577U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356459A (en) * | 2017-06-20 | 2017-11-17 | 潘沧桑 | The fast separation device of pine material nematode and its detection method of disassembling cleaning |
-
2011
- 2011-10-14 CN CN 201120390980 patent/CN202614577U/en not_active Expired - Lifetime
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107356459A (en) * | 2017-06-20 | 2017-11-17 | 潘沧桑 | The fast separation device of pine material nematode and its detection method of disassembling cleaning |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Assignee: NANJING SUNSHINE BIOTECHNOLOGY CO., LTD. Assignor: NanJing SunShine Biotechnology Co., Ltd. Contract record no.: 2014320000758 Denomination of utility model: Differential pressure method pine wood nematode quick separation device Granted publication date: 20121219 License type: Exclusive License Record date: 20141230 |
|
| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| ASS | Succession or assignment of patent right |
Owner name: SUNSHINE NANJING PEST CONSOLE OPERATION PCO TECHNO Free format text: FORMER OWNER: NANJING SUNSHINE BIOTECHNOLOGY CO., LTD. Effective date: 20150617 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20150617 Address after: 211100, Nanjing, Jiangsu province Jiangning District No. 605 Bamboo Road, Su Chi logistics three, two floor East Patentee after: NANJING SUNSHINE BIOTECHNOLOGY CO., LTD. Address before: 605 room 604 and 302, Zhonghua Road, Qinhuai District, Jiangsu, Nanjing 210006, China Patentee before: NanJing SunShine Biotechnology Co., Ltd. |
|
| C56 | Change in the name or address of the patentee | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 211100, Nanjing, Jiangsu province Jiangning District No. 605 Bamboo Road, Su Chi logistics three, two floor East Patentee after: Nanjing biological pest control technology, Limited by Share Ltd Address before: 211100, Nanjing, Jiangsu province Jiangning District No. 605 Bamboo Road, Su Chi logistics three, two floor East Patentee before: NANJING SUNSHINE BIOTECHNOLOGY CO., LTD. |
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| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20121219 |