CN1243963C - Criminal detection and case solving method using macroscopic and microscopic plant veins - Google Patents
Criminal detection and case solving method using macroscopic and microscopic plant veins Download PDFInfo
- Publication number
- CN1243963C CN1243963C CN 200310115807 CN200310115807A CN1243963C CN 1243963 C CN1243963 C CN 1243963C CN 200310115807 CN200310115807 CN 200310115807 CN 200310115807 A CN200310115807 A CN 200310115807A CN 1243963 C CN1243963 C CN 1243963C
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- Prior art keywords
- sample
- plant
- soaked
- line
- ethanol
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- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 8
- 238000001514 detection method Methods 0.000 title abstract description 5
- 210000003462 vein Anatomy 0.000 title abstract 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 241000196324 Embryophyta Species 0.000 claims abstract description 32
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims abstract description 18
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 12
- CTQNGGLPUBDAKN-UHFFFAOYSA-N O-Xylene Chemical compound CC1=CC=CC=C1C CTQNGGLPUBDAKN-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000007787 solid Substances 0.000 claims abstract description 8
- 230000018044 dehydration Effects 0.000 claims abstract description 7
- 238000006297 dehydration reaction Methods 0.000 claims abstract description 7
- 229960000583 acetic acid Drugs 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 6
- 239000012362 glacial acetic acid Substances 0.000 claims abstract description 6
- 239000008096 xylene Substances 0.000 claims abstract description 4
- 238000011840 criminal investigation Methods 0.000 claims description 7
- 235000019441 ethanol Nutrition 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 6
- 238000002360 preparation method Methods 0.000 claims description 6
- 239000000975 dye Substances 0.000 claims description 5
- 238000004043 dyeing Methods 0.000 claims description 5
- 235000013311 vegetables Nutrition 0.000 claims description 5
- 244000283070 Abies balsamea Species 0.000 claims description 3
- 235000007173 Abies balsamea Nutrition 0.000 claims description 3
- 239000004858 Canada balsam Substances 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- 238000006073 displacement reaction Methods 0.000 claims description 3
- 210000002615 epidermis Anatomy 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 229920001206 natural gum Polymers 0.000 claims description 3
- 150000007524 organic acids Chemical class 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- RZSYLLSAWYUBPE-UHFFFAOYSA-L Fast green FCF Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC(O)=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 RZSYLLSAWYUBPE-UHFFFAOYSA-L 0.000 claims description 2
- 239000003086 colorant Substances 0.000 abstract 1
- 238000007789 sealing Methods 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 230000002496 gastric effect Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 238000005336 cracking Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention discloses a criminal detection case solving method using macroscopic and microscopic plant veins, which comprises the following steps: a solid residue sample and a plant guide sample are put in a fixing agent prepared by ethanol, glacial acetic acid and formaldehyde to be fixed after being respectively put in a plant coloring agent to be soaked and dyed, the gradient soaking dehydration is carried out by the ethanol, the solid residue sample and the plant guide sample are transparently processed for being soaked by absolute alcohol and xylene mixed liquor and are finally processed by a Canada rubber sealing sheet, a sample plant vein photo and a guide sample plant vein photo are shot under a microscope, and the plant species of samples are determined by comparison. The plant vein photos have the characteristics of stable structure, non-easy damage, sufficient opinion evidence for case solution with plant vein application, high reliability, detection clue supply, detection range reduction and case solving rate increase.
Description
Technical field:
The present invention discloses a kind of criminal investigation plant both macro and micro and plants the line method of solving a case, and relates to the structural botany technical field.
Background technology:
At present, in criminal investigation, often utilize the solid residue of corpse gastric content, seek clue to solve the case, because solid residue is very broken and the effect of hydrochloric acid in gastric juice, commonsense method is difficult to determine food species, and can't directly take pictures and sample, fixing, preservation to it, therefore bring very big difficulty, influence the detection work of case to clear up a criminal case.
Summary of the invention:
The invention provides a kind of criminal investigation plant both macro and micro and plant the line method of solving a case, solved the problem that the solid residue of corpse gastric content in the criminal investigation work is difficult to differentiate.
Technical solution of the present invention may further comprise the steps:
1, dyeing: the solid residue sample is put into vegetable dyeing agent soaked 20～48 hours, vegetable dyeing agent comprises sarranine, bright green, fast green etc., make the cuticula and the secondary wall of planting line nucleus and outer tangential wall or plant the dyeing of line cell primary wall, increase its contrast effect;
2, fixing: taking out that sample puts into by capacity is that 88 parts 60 degree ethanol, 6 parts glacial acetic acid, 6 parts the fixing agent of formaldehyde preparation soaked 24～48 hours;
3, dehydration: it is that 50%, 60%, 70%, 80% to 95% ethanolic solution carries out gradient and soaks that above-mentioned sample is put into concentration again, each 2 hours, puts into absolute ethyl alcohol dehydration in 1 hour then;
4, transparent: as to use soaked in absolute ethyl alcohol sample 0.5 hour, put into the absolute ethyl alcohol and the dimethylbenzene mixed liquor after 1 hour of preparation in 1: 1 then, putting into xylene solution again soaked 1 hour, finish multiple organic acid and the displacement reaction of decomposing enzyme material in the sample, solve sample and plant the problem that line hazes easily;
5, take out the sample of handling well and carry out the mounting processing, take " sample is planted the line photo " at microscopically with Canada balsam or intermediate natural gum;
6, adopt above-mentioned 1～5 the step pair plant similar to handle to make standard specimen and plant the line photo with sample;
7, sample is planted line photo and standard specimen plant the line photo carry out pore type, guard cell's form, accessory cell form, type, and epidermis, pericarp membrane radial wall inlay the comparison of type, thereby determine the floristics of sample.
Above-mentioned fixing agent is mixed and made into by ratio of weight and the number of copies by following material:
60 degree ethanol 88～60, glacial acetic acid 6～4, formaldehyde 6～4.
Above-mentioned clarifier is mixed and made into by ratio of weight and the number of copies by following material:
Absolute ethyl alcohol 10, dimethylbenzene 10.
Good effect of the present invention is: " planting the line photo " has Stability Analysis of Structures, non-damageable characteristic, and it is not yielding to plant the line morphosis, uses therefore that to plant the line appraisal evidence of solving a case abundant, with a high credibility.By relatively determining sample type, give a clue for investigation, dwindle scope of investigation, improve the cracking of cases rate.The invention solves and how directly to take pictures after the corpse gastric content extracts and sample, fixing, the problem of preserving.
Embodiment:
At first take corpse gastric content solid residue sample and plant standard specimen, carry out steps of processing respectively:
1, dyeing: put into the sarranine vegetable dyeing agent and soaked 48 hours, the cuticula and the secondary wall of planting line nucleus and outer tangential wall dyeed, with bright green or fast greenly carry out dyeing in 1～6 second to planting line cell primary wall;
2, fixing: the fixing agent that the taking-up sample is put into by the formaldehyde preparation of the glacial acetic acid of the 60 degree ethanol of 88ml, 6ml, 6ml soaked 24 hours;
3, dehydration: it is that 50%, 60%, 70%, 80% to 95% ethanolic solution carries out gradient and soaks that above-mentioned sample is put into concentration again, each 2 hours, puts into absolute ethyl alcohol dehydration in 1 hour then;
4, transparent: as to use soaked in absolute ethyl alcohol sample 0.5 hour, put into the ethanol and the dimethylbenzene mixed liquor after 1 hour of preparation in 1: 1 then, putting into xylene solution again soaked 1 hour, finish multiple organic acid and the displacement reaction of decomposing enzyme material in the sample, solve sample and plant the problem that line hazes easily;
5, take out the sample of handling well and carry out the mounting processing, take " sample is planted the line photo " at microscopically with Canada balsam or intermediate natural gum;
6, adopt above-mentioned 1～5 the step pair plant similar to handle to make standard specimen and plant the line photo with sample;
7, sample is planted line photo and standard specimen plant the line photo carry out pore type, guard cell's form, accessory cell form, type, and epidermis, pericarp membrane radial wall inlay the comparison of type, thereby determine the floristics of sample.
Claims (2)
1, a kind of criminal investigation plant both macro and micro is planted the line method of solving a case, and it is characterized in that may further comprise the steps:
1) dyeing: the solid residue sample is put into vegetable dyeing agent soaked 20～48 hours, vegetable dyeing agent comprises sarranine, bright green, fast green;
2) fixing: the fixing agent that the taking-up sample is put into by ethanol, glacial acetic acid, formaldehyde preparation soaked 24～48 hours;
3) dehydration: it is that 50%, 60%, 70%, 80% to 95% ethanolic solution carries out gradient and soaks that above-mentioned sample is put into concentration again, each 2 hours, puts into absolute ethyl alcohol dehydration in 1 hour then;
4) transparent: as to use soaked in absolute ethyl alcohol sample 0.5 hour, put into the ethanol and the dimethylbenzene mixed liquor after 1 hour of preparation in 1: 1 then, putting into xylene solution again soaked 1 hour, finish multiple organic acid and the displacement reaction of decomposing enzyme material in the sample, solve sample and plant the problem that line hazes easily;
5) take out the sample of handling well and carry out the mounting processing, take " sample is planted the line photo " at microscopically with Canada balsam or intermediate natural gum;
6) adopt above-mentioned 1～5 the step pair plant similar to handle to make standard specimen and plant the line photo with sample;
7) sample is planted line photo and standard specimen plant the line photo carry out pore type, guard cell's form, accessory cell form, type, and epidermis, pericarp membrane radial wall inlay the comparison of type, thereby determine the floristics of sample.
2, criminal investigation plant both macro and micro according to claim 1 is planted the line method of solving a case, and it is characterized in that: used fixing agent is mixed and made into by ratio of weight and the number of copies by following material:
60 degree ethanol 88, glacial acetic acid 6, formaldehyde 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310115807 CN1243963C (en) | 2003-11-26 | 2003-11-26 | Criminal detection and case solving method using macroscopic and microscopic plant veins |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200310115807 CN1243963C (en) | 2003-11-26 | 2003-11-26 | Criminal detection and case solving method using macroscopic and microscopic plant veins |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1544908A CN1544908A (en) | 2004-11-10 |
| CN1243963C true CN1243963C (en) | 2006-03-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200310115807 Expired - Fee Related CN1243963C (en) | 2003-11-26 | 2003-11-26 | Criminal detection and case solving method using macroscopic and microscopic plant veins |
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| Country | Link |
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| CN (1) | CN1243963C (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105651573B (en) * | 2016-01-05 | 2018-05-01 | 中国科学院华南植物园 | One Leaf Epidermis hair removal methods |
| CN106294728A (en) * | 2016-08-08 | 2017-01-04 | 江勇 | The construction method of androphile's information bank and system thereof |
| CN110108706A (en) * | 2019-05-24 | 2019-08-09 | 东北师范大学 | The plant line identification method of the small relic of rotted plant material in a kind of soil corpse |
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2003
- 2003-11-26 CN CN 200310115807 patent/CN1243963C/en not_active Expired - Fee Related
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| Publication number | Publication date |
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| CN1544908A (en) | 2004-11-10 |
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Owner name: NORTHEAST NORMAL UNIVERSITY Free format text: FORMER OWNER: LU JINGMEI Effective date: 20070406 |
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Effective date of registration: 20070406 Address after: 130024 No. 5268 Renmin Street, Changchun Patentee after: Northeast Normal University Address before: 130024 School of life science, Northeast Normal University, 138 Renmin Street, Changchun, Jilin Patentee before: Lu Jingmei |
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Granted publication date: 20060301 |