CN101285212A - Degumming process for mulberry fibre by bacilli compound bacteria - Google Patents
Degumming process for mulberry fibre by bacilli compound bacteria Download PDFInfo
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- CN101285212A CN101285212A CNA2008100735962A CN200810073596A CN101285212A CN 101285212 A CN101285212 A CN 101285212A CN A2008100735962 A CNA2008100735962 A CN A2008100735962A CN 200810073596 A CN200810073596 A CN 200810073596A CN 101285212 A CN101285212 A CN 101285212A
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- degumming
- composite flora
- mulberry
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Abstract
The invention provides a method for degumming mulberry fiber with bacteria complex flora. The method uses mulberry as a culture medium for a carbon source to filter the bacteria complex flora with good degumming effect to the mulberry from sludge of a methane tank, substrate sludge of a pool, wood, banana land and other samples, has dried mulberry directly immersed into degumming liquid, and inoculates the cultured degumming complex bacterial flora into the degumming liquid for degumming, so that loose mulberry fiber can be obtained via water rinsing and drying. The method has the advantages of mild process condition and simple operation, need not use strong acid or strong alkali, has the manufacturing cost greatly reduced compared with a chemical degumming method, has lower environmental pollution, less cull in the prepared mulberry fiber and white and loose fiber.
Description
One, technical field
The invention belongs to the method that a kind of mulberry fibre comes unstuck, particularly a kind of method of mulberry fibre being come unstuck with the bacterium composite flora.
Two, technical background
China is topmost in the world kind of Sang Yangcan country, national mulberry field area was 1165.3 ten thousand mu in 2005, and 2006 is 1,259 ten thousand mu, and 2007 is 1440.99 ten thousand mu, mulberry leaf are used for breeding silkworms, and annual a large amount of branches of cutting down are not also well utilized so far.Mulberry fibre contained in the mulberry skin had both had the characteristic of cotton, had many advantages of flax fibre again.Mulberry fibre has good hygroscopicity, gas permeability, warmth retention property and certain health-care effect, and its gloss is good, soft, be easy to dyeing, is a kind of fully natural green fiber of high added value.Develop natural mulberry fibre as textile material, not only can turn waste into wealth, discarded mulberry branch is fully utilized, and increased the kind of textile product, improved the class of textile garment product, can also increase income, therefore good economic and social benefit has been arranged for the peasant.But mulberry fibre content is lower in the mulberry skin, 10-30% only, and the content of hemicellulose, lignin and pectin is higher, mulberry fibre is interweaved with lignin with pectin, hemicellulose under native state and is in the same place, therefore, how extracting fiber-spinnable effectively is the key point that mulberry fibre utilizes.Producing exactly of mulberry fibre will be removed with hemicellulose, lignin and pectin that mulberry fibre is interweaved by Degumming method, and mulberry fibre is discharged.Because the research to mulberry fibre also is in initial stage at present, the degumming tech of mulberry fibre has followed the technology of retting, mainly uses chemical degumming technique.The chemical degumming law energy consumption is big, and the output capacity of fiber is lower, and short flannel is more, also can damage fiber surface, also can cause severe contamination to environment simultaneously, does not meet the theory of the ecological, environmental protective harmonious development of modern society.Mulberry skin microbial degumming technology is to utilize degrade the fast technology of mulberry skin colloid composition of the enzyme of microorganisms, its treatment conditions gentleness, less to fibre damage, energy consumption is low, pollute few, can improve the mulberry fibre quality, reduce production costs, be the inexorable trend of producing the sustainable development of mulberry fibre industry.Mulberry skin microbial degumming technology is actually under the acting in conjunction of pectase, hemicellulase and lignoenzyme that microorganism species produces, depolymerized pectin, hemicellulose and lignin progressively discharge the process of mulberry fibre, and this is the process that single microorganism is difficult to finish.Therefore, set up one and overlap mulberry skin microbial degumming technology efficiently, must obtain a microorganism species that can produce the eurythmy of highly active pectase, hemicellulase and lignoenzyme and various enzymes.
Three, summary of the invention
The purpose of this invention is to provide a kind of method of mulberry fibre being come unstuck with the bacterium composite flora, this method can be effectively, economy, environmental protection are carried out mulberry fibre and come unstuck.
The present invention achieves the above object by the following technical programs: a kind of with the bacterium composite flora to the method that mulberry fibre comes unstuck, may further comprise the steps:
(1) come unstuck bacterium composite flora seed liquor preparation: the bacterium composite flora preservative fluid that will come unstuck inserts in the bacterium composite flora nutrient solution that comes unstuck by the inoculum concentration of 5-10% (v/v), cultivated 24-48 hour down in 30-37 ℃, pH7.0-8.0,
(2) in dried mulberry skin: degumming baths is 1: the ratio of 20-30 adds degumming baths in the dried mulberry skin,
(3) inoculum concentration by 10-15% (v/v) inserts the cultured bacterium composite flora seed culture fluid that comes unstuck,
(4) 35-40 ℃, pH 7.5-8.5 soak down and came unstuck in 48-72 hour,
(5) with clear water the mulberry fibre after coming unstuck is carried out rinsing, in drying under the sunlight or about 60 ℃ oven dry down.
The composition of described degumming baths and the bacterium composite flora nutrient solution that comes unstuck composition are respectively:
(1) composition of degumming baths (w/v): 0.2-0.5% urea liquid, or 0.2-0.5% (NH
4)
2SO
4Solution,
(2) the come unstuck composition (w/v) of bacterium composite flora nutrient solution: 0.2-0.5% carbon source, 0.2-0.5% nitrogenous source.Carbon source wherein is any one of pectin, wood sugar, xylan, and nitrogenous source is urea, KNO
3, NH
4Cl, (NH
4)
2SO
4Any one.
The screening technique of described bacterium composite flora is as follows:
From containing the local pedotheque of gathering such as the methane-generating pit mud that enriches pectic substance, sediment of pond, the woods, banana ground, be positioned in the enrichment culture liquid composition of enrichment culture liquid (w/v) respectively: dried mulberry skin 5%, (NH
4)
2SO
40.5%, static cultivation under 37 ℃, pH8.0, observe the content of pectase, zytase and the lignoenzyme of fiber release conditions and mensuration nutrient solution, select the culture that degumming effect is good, enzyme content is high, get the 50ml nutrient solution, change in the new enrichment culture liquid of 450ml 37 ℃ of static cultivations over to, after 5 enrichments, obtain the good bacterium composite flora of degumming effect.
(2) preservation
The aseptic glycerine of getting 50mL bacterium composite flora nutrient solution and 50mL concentration and be 30% (w/v) mixes, and is loaded in the aseptic plastic bottle, shakes up, and preserves in-20 ℃ of refrigerators.
Technological merit of the present invention is as follows:
What 1, use is that the bacterium composite flora that can produce pectase, zytase, lignoenzyme etc. comes unstuck, can not only effectively remove pectic substance, also can effectively remove other non-cellulose materials such as hemicellulose, lignin, to compare its residual gum content low with chemical degumming law and other bacterial degumming method, and degumming effect is good.
2, the constituent of bacterium composite flora nutrient solution of Shi Yonging and degumming baths is simple, economical, and degumming tech is simple, easy to operate, both can reduce production costs greatly, can reduce labor intensity again.
3, whether the mild condition of coming unstuck of Shi Yonging or not strong acid and strong base and high temperature, little to the damage of mulberry fibre, and the fibre strength height had both improved quality of fibre, can alleviate equipment corrosion again, reduce environmental pollution.
Four, the specific embodiment
The present invention will be further described below in conjunction with specific embodiment.
Embodiment 1
1, enriched medium is formed (w/v): 5% dried mulberry skin, 0.5% (NH
4)
2SO
4
Screening technique: from containing the local pedotheque of gathering such as the methane-generating pit mud that enriches pectic substance, sediment of pond, the woods, banana ground, be positioned in the enriched medium respectively, 37 ℃, pH 8.0 static cultivations, observe the content of pectase, zytase and the lignoenzyme of fiber release conditions and mensuration nutrient solution, select the culture that degumming effect is best, enzyme content is high, get the 50ml nutrient solution, change in the new enriched medium of 450ml, 37 ℃ of static cultivations, after 5 enrichments, obtain the good bacterium composite flora of degumming effect.
2, the bacterium composite flora that comes unstuck is cultivated
The composition of nutrient solution (w/v): pectin 0.5%, urea 0.2%.
Raise craft condition: the bacterium composite flora that comes unstuck that will screen is inoculated in the bacterium composite flora nutrient solution that comes unstuck, and cultivates down in 37 ℃, pH8.0 and promptly gets the bacterium composite flora seed culture fluid that comes unstuck in 24 hours.
3, come unstuck
Degumming baths is formed (w/v): 0.2% urea liquid.
Degumming tech: in dried mulberry skin: degumming baths is that 1: 25 ratio adds degumming baths in the dried mulberry skin, inserts the cultured bacterium composite flora seed culture fluid that comes unstuck by the inoculum concentration of 10% (v/v), and 37 ℃, pH8.0 soak down and came unstuck in 72 hours.
4, rinsing drying process
With clear water the mulberry fibre after coming unstuck is carried out rinsing, under sunlight, dry or dry.
The clearance of non-cellulose material is 82.3% (w/w) as a result, and residual gum content is 1.51%, and filament intensity is 7.02cN/dtex.
Embodiment 2
1, the come unstuck screening of bacterium composite flora
Adopt method enrichment and the screening identical with embodiment 1.
2, the bacterium composite flora that comes unstuck is cultivated
The composition of nutrient solution (w/v): 0.5% xylan, 0.5%KNO
3
Raise craft condition: the bacterium composite flora that comes unstuck that will screen is inoculated in the bacterium composite flora nutrient solution that comes unstuck, and cultivates down in 35 ℃, pH7.0 and promptly gets the bacterium composite flora seed liquor of coming unstuck in 48 hours.
3, come unstuck
Degumming baths is formed (w/v): 0.5% (NH
4)
2SO
4Solution.
Degumming tech: in dried mulberry skin: degumming baths is that 1: 25 ratio adds degumming baths in the dried mulberry skin, inserts the cultured bacterium composite flora seed liquor of coming unstuck by the inoculum concentration of 15% (v/v), and 40 ℃, pH8.0 soak down and came unstuck in 48 hours.
4, rinsing drying process
With clear water the mulberry fibre after coming unstuck is carried out rinsing, under sunlight, dry or dry.
The clearance of non-cellulose material is 81.1% as a result, and residual gum content is 1.73%, filament intensity 7.11cN/dtex.
Embodiment 3
1, the come unstuck screening of bacterium composite flora
Adopt method enrichment and the screening identical with embodiment 1.
2, the bacterium composite flora that comes unstuck is cultivated
The composition of nutrient solution (w/v): wood sugar 0.2%, urea 0.5%.
Raise craft condition: the bacterium composite flora that comes unstuck that will screen is inoculated in the bacterium composite flora nutrient solution that comes unstuck, and cultivates down in 35 ℃, pH7.5 and promptly gets the bacterium composite flora seed culture fluid that comes unstuck in 36 hours.
3, come unstuck
Degumming baths is formed (w/v): 0.2% urea liquid.
Degumming tech: in dried mulberry skin: degumming baths is that 1: 30 ratio adds degumming baths in the dried mulberry skin, inserts the bacterium composite flora seed liquor of coming unstuck by the inoculum concentration of 15% (v/v), and 35 ℃, pH8.5 soak down and came unstuck in 60 hours.
4, rinsing drying process
With clear water the mulberry fibre after coming unstuck is carried out rinsing, under sunlight, dry or dry.
The clearance of non-cellulose material is 79.3% as a result, and residual gum content is 1.97%, filament intensity 7.85cN/dtex.
Embodiment 4
1, the come unstuck screening of bacterium composite flora
Adopt method enrichment and the screening identical with embodiment 1.
2, the bacterium composite flora that comes unstuck is cultivated
The composition of nutrient solution (w/v): pectin 0.2%, (NH
4)
2SO
40.5%.
Raise craft condition: the bacterium composite flora that comes unstuck that will screen is inoculated in the bacterium composite flora nutrient solution that comes unstuck, and cultivates down in 30 ℃, pH7.5 and promptly gets the bacterium composite flora seed liquor of coming unstuck in 24 hours.
3, come unstuck
Degumming baths is formed (w/v): 0.2% (NH
4)
2SO
4Solution.
Degumming tech: in dried mulberry skin: degumming baths is that 1: 20 ratio adds degumming baths in the dried mulberry skin, inserts the bacterium composite flora seed liquor of coming unstuck by the inoculum concentration of 15% (v/v), and 37 ℃, pH7.5 soak down and came unstuck in 72 hours.
4, rinsing drying process
With clear water the mulberry fibre after coming unstuck is carried out rinsing, under sunlight, dry or dry.
The clearance of non-cellulose material is 78.2% as a result, and residual gum content is 2.12%, filament intensity 7.91cN/dtex.
Embodiment 5
1, the come unstuck screening of bacterium composite flora
Adopt method enrichment and the screening identical with embodiment 1.
2, the bacterium composite flora that comes unstuck is cultivated
The composition of nutrient solution (w/v): wood sugar 0.5%, urea 0.2%.
Raise craft condition: the bacterium composite flora that comes unstuck that will screen is inoculated in the bacterium composite flora nutrient solution that comes unstuck, and cultivates down in 37 ℃, pH8.0 and promptly gets the bacterium composite flora seed liquor of coming unstuck in 48 hours.
3, come unstuck
Degumming baths is formed (w/v): 0.5% urea liquid.
Degumming tech: in dried mulberry skin: degumming baths is that 1: 30 ratio adds degumming baths in the dried mulberry skin, inserts the bacterium composite flora seed culture fluid that comes unstuck by the inoculum concentration of 10% (v/v), and 40 ℃, pH soak for 7.5 times and came unstuck in 72 hours.
4, rinsing drying process
With clear water the mulberry fibre after coming unstuck is carried out rinsing, under sunlight, dry or dry.
The clearance of non-cellulose material is 75.6% as a result, and residual gum content is 2.51%, filament intensity 7.56cN/dtex.
The mensuration of dried mulberry skin component is to adopt the quantitative analysis method of standard GB 5889-86 ramie chemical composition to measure, chemical composition (the % of dried after measured mulberry skin, w/w) be adipocere matter 0.78, the hydrotrope 15.72, cellulose 14.03, pectic substance 11.91, hemicellulose 34.90, lignin 22.66.
Residual gum content and filament intensity adopt standard GB/T5881-1986 " ramie physicochemical property test sampling method " to measure.The clearance of non-cellulose material adopts gravimetric detemination, i.e. the clearance of non-cellulose material (%)=100 * (mulberry skin weight before the coming unstuck-back mulberry fibre weight of coming unstuck)/mulberry skin weight before coming unstuck.
Claims (4)
1, a kind of with the bacterium composite flora to the method that mulberry fibre comes unstuck, it is characterized in that said method comprising the steps of:
(1) come unstuck bacterium composite flora seed liquor preparation: the bacterium composite flora preservative fluid that will come unstuck inserts in the bacterium composite flora nutrient solution that comes unstuck by the inoculum concentration of 5-10% (v/v), cultivated 24-48 hour down in 30-37 ℃, pH7.0-8.0,
(2) in dried mulberry skin: degumming baths is 1: the ratio of 20-30 adds degumming baths in the dried mulberry skin,
(3) inoculum concentration by 10-15% (v/v) inserts the cultured bacterium composite flora seed liquor of coming unstuck,
(4) 35-40 ℃, pH 7.5-8.5 soak down and came unstuck in 48-72 hour,
(5) with clear water the mulberry fibre after coming unstuck is carried out rinsing, under sunlight, dry or dry.
2, according to claim 1 with the bacterium composite flora to the method that mulberry fibre comes unstuck, it is characterized in that the composition of described degumming baths and the bacterium composite flora nutrient solution that comes unstuck is formed and to be respectively:
(1) composition of degumming baths (w/v): 0.2-0.5% urea liquid, or 0.2-0.5% (NH
4)
2SO
4Solution,
(2) the come unstuck composition (w/v) of bacterium composite flora nutrient solution: 0.2-0.5% carbon source, 0.2-0.5% nitrogenous source.
3, according to claim 2 with the bacterium composite flora to the method that mulberry fibre comes unstuck, it is characterized in that described carbon source is any one of pectin, wood sugar, xylan, nitrogenous source is urea, KNO
3, NH
4Cl, (NH
4)
2SO
4Any one.
4, according to claim 1 with the bacterium composite flora to the method that mulberry fibre comes unstuck, it is characterized in that described bacterium composite flora obtains by the following method:
(1) screening
From containing the local pedotheque of gathering such as the methane-generating pit mud that enriches pectic substance, sediment of pond, the woods, banana ground, be positioned in the enrichment culture liquid composition of enrichment culture liquid (w/v) respectively: dried mulberry skin 5%, (NH
4)
2SO
40.5%, static cultivation under 37 ℃, pH8.0, observe the content of pectase, zytase and the lignoenzyme of fiber release conditions and mensuration nutrient solution, select the culture that degumming effect is good, enzyme content is high, get the 50ml nutrient solution, change in the new enrichment culture liquid of 450ml 37 ℃ of static cultivations over to, after 5 enrichments, obtain the good bacterium composite flora of degumming effect.
(2) preservation
The aseptic glycerine of getting 50mL bacterium composite flora nutrient solution and 50mL concentration and be 30% (w/v) mixes, and is loaded in the aseptic plastic bottle, shakes up, and preserves in-20 ℃ of refrigerators.
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| CN104451900A (en) * | 2014-11-18 | 2015-03-25 | 柳江县联华纸制品厂 | Method for degumming mulberry bast fiber |
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| CN102174399A (en) * | 2010-12-31 | 2011-09-07 | 广西赛尔纸业有限公司 | Method for preparing herbal biological fiber and biological paper pulp |
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| CN104451900A (en) * | 2014-11-18 | 2015-03-25 | 柳江县联华纸制品厂 | Method for degumming mulberry bast fiber |
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| CN105239168A (en) * | 2015-11-03 | 2016-01-13 | 长兴超凡纺织有限公司 | Preparation method of antibacterial environment-friendly mulberry bark fiber |
| CN105239167A (en) * | 2015-11-03 | 2016-01-13 | 长兴超凡纺织有限公司 | High strength modified mulberry bark fiber |
| CN105239233A (en) * | 2015-11-03 | 2016-01-13 | 长兴超凡纺织有限公司 | Antibacterial mulberry fiber cotton blended yarn |
| CN105239167B (en) * | 2015-11-03 | 2018-05-22 | 宁波凯丽安科技股份有限公司 | A kind of high intensity is modified mulberry fibre |
| CN105239168B (en) * | 2015-11-03 | 2019-01-22 | 浙江品创知识产权服务有限公司 | A kind of mulberry fibre of antibacterial environment protection |
| CN106632637A (en) * | 2017-01-03 | 2017-05-10 | 王玲燕 | Mulberry silk protein extraction method |
| CN106749753A (en) * | 2017-01-03 | 2017-05-31 | 王玲燕 | A kind of biological alginate preparation method |
| CN107523885A (en) * | 2017-07-06 | 2017-12-29 | 温美群 | A kind of mulberry tree hide fiber extracting method |
| CN108588854A (en) * | 2018-07-01 | 2018-09-28 | 李冬生 | A kind of preparation method of mulberry fibre |
| CN112593433A (en) * | 2020-11-09 | 2021-04-02 | 安徽省农业科学院蚕桑研究所 | Papermaking method using mulberry bark as raw material |
| CN112593433B (en) * | 2020-11-09 | 2023-03-10 | 安徽省农业科学院蚕桑研究所 | Papermaking method using mulberry bark as raw material |
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